2022 · 2023 which Lead to Success of Your T omorrow. BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies Beyond the PCR T echnology, BIOFACT Promises the Progress for Your Research.
BIOFACT
Catalogue
Beyond the FACT, BIOFACT
2022 · 2023 BIOFACT CATALOGUE
㈜바이오팩트 / ㈜바이오팩트 생명공학연구소
대전본사 I 34124 대전광역시 유성구 엑스포로 240 대표번호 1670-5695 Tel. 042-867-5695 Fax. 042-825-5695
서울지사 I 04788 서울특별시 성동구 광나루로 130 서울숲 IT캐슬 1008호 Tel. 02-851-5695 Fax. 02-851-5690
www.bio-ft.com I www.biocart.co.kr(쇼핑몰) I [email protected]
Beyond the FACT, BIOFACT
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
DNA/RNA Amplification
PCR Description, Real-Time PCR Enzyme
PCR Enzyme, RT & RT-PCR Enzyme, Other Reagent
DNA/RNA Preparation
Prep Kit Description, Genomic DNA Extraction
Total RNA Extraction, Plasmid Extraction
PCR Product Purification
Ligase &
Electrophoresis Reagent
Ligase, Electrophoresis Reagent, Pre-made buffer
Lab Supplies
Plasticware, Other Supplies
Filtration
Mini Column, Syringe Filter, Membrane Filter
Contents
About BIOFACT 06 RT & RT-PCR Enzyme
BioFACT™ RT Series 102
BioFACT™ OneStep RT-PCR Series 104
DNA/RNA Amplification
Isothermal Amplification
Description 18 BioFACT™ Bst DNA Polymerase 108
Lyophilized PCR Enzyme Other Reagent
LyoFACT™ PCR Pre-Mix Lyophilisates 40 BioFACT™ Uracil DNA Glycosylase (UDG) 112
LyoFACT™ RT Pre-Mix Lyophilisates 42 BioFACT™ dNTP and dNTP Set 113
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates 44 5X Band Helper™ 114
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (For Probe) 46 BioFACT™ RNase Inhibitor 116
LyoFACT™ All in one PCR Pre-Mix Lyophilisates 48
Real-Time PCR Enzyme
Real-Time PCR 52 DNA/RNA Preparation
BioFACT™ Real-Time PCR Series 56
SFCgreen® I 57 DNA Prep Kit Description 120
BioFACT™ Real-Time PCR Series (With SFCgreen® I) 59
BioFACT™ Real-Time PCR Series (With SYBR® Green I) 64 Magnetic Bead / Stand / Pipettor
BioFACT™ Real-Time PCR Series (With EvaGreen™) 65 DaBead™ Magnetic Bead 124
20X EvaGreen™ 66 DaBead™ Magnetic Separation Stand 126
BioFACT™ OneStep qRT-PCR Master Mix 67 DaBead™ Magnetic Bead Press Pipettor 128
FastFACT™ Real-Time PCR Master Mix 69 DaBead™ Magnetic Bead Pipettor 130
BioFACT™ Multi-Star Real-Time PCR Master Mix 70
BioFACT™ OneStep Multi-Star qRT-PCR Series 72 Viral RNA/DNA Extraction
FastFACT™ qPCR Master Mix 74 DaBead™ Viral RNA/DNA Prep Kit [Magnetic Bead Type] 132
FastFACT™ qRT-PCR Master Mix 75 HiGene™ Viral RNA/DNA Prep Kit [Column Type] 136
PCR Enzyme Genomic DNA Extraction
BioFACT™ F-Star Taq DNA Polymerase (Hot Start) 78 DaBead™ Genomic DNA Prwep Kit For Bacterium, Cultured cell, 138
Whole Blood, Animal Tissue, Saliva, Oral Epithelial Cell, Hair Root
BioFACT™ Thumb Taq DNA Polymerase 80
[Magnetic Bead Type]
BioFACT™ Taq DNA Polymerase 82
DaBead™ Genomic DNA Prep Kit For Plant [Magnetic Bead Type] 143
BioFACT™ H-Star Taq DNA Polymerase (Hot Start) 84
DaBead™ GMO DNA Extraction Kit I For Potato, Bean, Maize 148
BioFACT™ A-Star Taq DNA Polymerase (Hot Start) 86 [Magnetic Bead Type]
BioFACT™ Lamp Taq DNA Polymerase 88 DaBead™ Genomic DNA Extraction Kit For Rice 151
[Magnetic Bead Type]
BioFACT™ A-Star Lamp Taq DNA Polymerase (Hot Start) 90
DaBead™ Cell Free DNA Prep Kit For Plasma, Serum 153
BioFACT™ Pfu DNA Polymerase 92 [Magnetic Bead Type]
BioFACT™ Lamp Pfu DNA Polymerase 94 HiGene™ Genomic DNA Prep Kit [Column Type] 155
BioFACT™ 2X Multi-Star PCR Series (Hot Start) 96 HiGene™ Genomic DNA Prep Kit For Microorganisms 159
[Grinding Bead & Column Type]
SimpleWay™ 2X PCR Master Mix III 98
HiGene™ Genomic DNA Prep Kit For Soil 162 Lab Supplies
[Grinding Bead & Column Type]
HiGene™ Genomic DNA Prep Kit [Solution Type] 165
Plasticware
SimpleWay™ gDNA Prep & PCR Set [Easy Type] 169
96 Well Cell Culture Plate 214
Total RNA Extraction Sealing Material & PCR Plate 215
PCR Tube (Conventional) 216
DaBead™ Total RNA Prep Kit [Magnetic Bead Type] 171
PCR Tube (Real-time) 217
HiGene™ Total RNA Prep Kit [Column Type] 174
PCR Tube Rack 218
Plasmid Extraction Micro Tube 219
DaBead™ Plasmid Prep Kit [Magnetic Bead Type] 177
Other Supplies
HiGene™ Plasmid Mini Prep Kit (Ver.2.0) [Column Type] 174
Al Cooling Block 224
PCR Product Purification
DaBead™ PCR Purification Kit [Magnetic Bead Type] 182
DaBead™ Size-Selective Cleanup Kit [Magnetic Bead Type] 186
Filtration
DaBead™ Gel Extraction Kit [Magnetic Bead Type] 188
DaBead™ Sequencing Reaction Clean Up Kit 190
[Magnetic Bead Type] Mini column
HiGene™ Gel & PCR Purification System [Column Type] 192 DNA/RNA Purification Mini Column 228
HiFilter (for removing debris) 228
Syringe filter
Ligase & CA (Cellulose Acetate) 231
Electrophoresis Reagent MCE (Mixed Cellulose Ester) 231
PES (Polyethersulfone) 231
Ligase Nylon (Polyamide) 231
BioFACT™ T4 DNA Ligase 198 PVDF (Polyvinylidene Fluoride) 231
PTFE – W (Polytetrafluoroethylene - Hydrophilic) 231
Electrophoresis Reagent PTFE – O (Polytetrafluoroethylene-Hydrophobic) 231
gel-rose™ Agarose 199
EcoDye™ Nucleic Acid Staining Solution 201 Membrane filter
BioFACT™ 1kb Plus DNA Ladder 204 MCE (Mixed Cellulose Ester) 237
BioFACT™ 100 bp Plus DNA Ladder 204 PES (Polyethersulfone) 237
BioFACT™ 6X Loading Dye 204 Nylon (Polyamide) 237
BioFACT ™ Triple Color Protein Marker (10 ~ 180 kDa) 207 PVDF (Polyvinylidene Fluoride) 237
BioFACT ™ Triple Color Protein Marker (10 ~ 245 kDa) 207 PTFE (Polytetrafluoroethylene - Hydrophobic) 237
HiGene™ Pre-made buffer 210
Beyond the FACT,
BIOFACT
BIOFACT is a leading biotechnology company that provides total solution required from basic
experiment to sophisticated and complex analysis. We have manufactured and offered a wide
range of products such as PCR enzymes, DNA/RNA preparation kits and other molecular biolog-
ical reagents to our customers as well as supplying various instruments and services which are
associated with their research. Furthermore, BIOFACT has been discovering diverse products of
Korean and global brands in order to provide the optimal items which can meet the needs of our
customers. And then, we supply them through our wide and powerful distribution network which
was built up over a long period of time.
Recently BIOFACT launched experimental education program (Experiment Academy Service)
for middle and high school students. Through this academy service conducted by professionals,
we contribute to foster and develop the future research experts by providing specialized educa-
tion as well as offering excitement of academic advancement.
In keeping with the successful growth and expansion, BIOFACT is committed to constantly im-
proving the products and services to enable our customers to achieve their goals in all areas of
biotechnology, life and medical science. Well-trained staffs of BIOFACT make every effort for sat-
isfying needs of our customers and offer the best assistance to our customers through custom-
ized consulting services while establishing and maintaining internal systems (marketing, sales,
manufacturing, development and quality control).
BIOFACT strives to offer reliable products and services enabling exceptional applications in life
science. We will help you to accelerate your research and breakthrough your challenges.
BIOFACT contributes to your success and better tomorrow.
6 BIOFACT CATALOGUE
About BIOFACT
BIOFACT Web Site
BIOFACT Partners
Human-Centered Customer Satisfaction Shared Growth
Management Management Management
BIOFACT creates a positive organi- BIOFACT strives to offer reliable BIOFACT founded on the principles
zational culture by providing oppor- products and exceptional services of fairness & transparency operates
tunities for all staff to develop their that help our customers to accel- various shared growth programs
own abilities and make of their cre- erate their researches and break- with our partners for sustainable
ativity. through their challenges. management.
7
BIOFACT CATALOGUE
BIOFACT BIOFACT makes steady efforts towards the best quality.
BIOFACT operates a thoroughly standardized product and service system through
CERTIFICATE international standardization examination that includes product or service quality, op-
erational efficiency, environmental performance, health and safety in the workplace
and many more.
ISO13485 : 2016
ISO13485 : 2016 Confirmation of Genetic Confirmation of
Testing Agency Venture Business
8 BIOFACT CATALOGUE
About BIOFACT
BIOFACT Web Site
BIOFACT Partners
Certificate of Medical Device Certificate of
R&D Center Manufacturing License Inno-Biz
9
BIOFACT CATALOGUE
BIOFACT Headquarter, Biotechnology Research Institute
Genetic Analysis Laboratory / Seoul Office
BIOFACT Contact
Tel. +82-70-7826-5453 Fax. + 82-42-385-5511 E-mail : [email protected]
HEAD OFFICE
2/3F, 240 EXPO-ro, Yuseong-gu, Daejeon, 34124 Republic Korea
SEOUL OFFICE
130, Gwangnaru-ro, Seongdong-gu, Seoul, 04788 Republic of Korea
10 BIOFACT CATALOGUE
About BIOFACT
BIOFACT Web Site
BIOFACT Partners
Meet new item & update
news of BIOFACT everyday
BIOFACT WEB SITE
Main website (Kor.) www.bio-ft.com
Global website (Eng.) www.bio-ft.com/eng
Sequencing /
Microorganism identification on-line site www.genefact.net
Facebook www.facebook.com/snc.biofact
On-line community cafe.naver.com/biofactacademy
11
BIOFACT CATALOGUE
• BIOFACT Partners (Domestic)
12 BIOFACT CATALOGUE
About BIOFACT
BIOFACT Web Site
BIOFACT Partners
Good Business
Partner
In order to provide the optimum products in the rapid-
ly-changing biotech industry, it is providing new products &
services through the stably-built nationwide sales distribu-
tion network by discovering/developing various products
from domestic/foreign manufacturers. In addition, for our
international partners, BIOFACT Co., Ltd not only developed
the best products and provided customized consulting ser-
vices by assigning the professional manpower through the
strategic concentration on the bio-related industry, but also
increased the product quality and service competitiveness.
• BIOFACT Partners (Global)
COUNTRY COMPANY TEL E-MAIL
Armenia Organix LLC +37433486487 [email protected]
Indonesia PT Setara Bio Science +622122176021 [email protected]
Malaysia Gene Xpress PLT +60358796317 [email protected]
Saudi Arabia Asila Riyadh Company +966118103078 [email protected]
Thailand Smart Science Co., Ltd. +6620777593 [email protected]
Vietnam Dai Xuan Biomedical Joint Stock Company +84329045957 [email protected]
13
BIOFACT CATALOGUE
BIOFACT
Molecular Biology Workflow
DNA Isolation PCR Purification
• Magnetic bead Type • Lyophilized PCR
- Viral RNA/DNA - PCR, RT, Onestep RT-PCR, qRT-PCR • Magnetic bead Type
- Bacterium, Cultured cell - PCR (With Primer) - PCR puri cation kit
- Whole blood - Gel extraction kit
- Plant tissue, Rice, Seed, GMO • Real-Time PCR
- Animal tissue - Intercalating dye (SFCgreen®Ⅰ,
- Saliva, Oral epithelial cell, Hair root SYBR® GreenⅠ, EvaGreen™) • Column Type
- Cell free DNA - Probe (Multi-Star Real-Time PCR) - Gel & PCR puri cation kit
- Semi-auto/Auto genomic DNA prep kit
- Fast qPCR
• Column Type • Conventional PCR
- Viral RNA/DNA - Universal PCR
- Whole blood, Bacterium, Plant tissue, (F-Star Taq, Thumb Taq, Taq)
Animal tissue, Fungus
- Yeast - Gene speci c PCR
(H-Star Taq, A-Star Taq)
• Solution Type - Long PCR (Lamp Taq, A-Star Lamp Taq)
- Whole blood - High delity (Pfu, Lamp Pfu)
- Bacterium - Muliplex (2X Multi-Star Taq)
- Plant tissue - Low inhibition
- Animal tissue , Fungus (SimpleWay™ 2X PCR Master Mix III)
- Yeast
- Isothermal (Bst)
• Easy Type - Host DNA-Free PCR (Launch planned)
- Whole blood, Animal tissue, • Other
Saliva hair root, Oral epithelial cell
- UDG, dNTP, 5X Band Helper™
- RNase inhibitor
RNA Isolation RT / PCR
• Magnetic bead Type • Reverse Transcription
- Bacterium, Animal tissue, - RT series
Cultured cell
- Plant tissue • OneStep RT-PCR
- OneStep RT-PCR series
• Column Type
- Plant tissue, Animal tissue, • qRT-PCR
Bacterium, Culture cell
- OneStep Multiplex qRT-PCR series
- Fast qRT-PCR
14 BIOFACT CATALOGUE
Cloning Plasmid Prep Sequencing
• Vector • Magnetic bead Type • Sequencing
- PCR cloning kit Mini, midi & maxi prep - Sanger sequencing
- Plasmid prep kit - Primer walking (Full SEQ)
• Competent Cell (Cell debris removal by centrifuge)
- Competent cell (DH5α ) • Microbial Identification
Midi & maxi prep - 16S & 18S rRNA identi cation full service
• Ligase - Plasmid prep kit - MLST
- T4 DNA ligase (Cell debris removal by syringe lter)
• Genotyping Service
- Only running, MS, STR, SSR, VNTR,
• Column Type ASP, AFLP, T-RFLP3
- Plasmid mini prep kit
• Customized Service
- PCR, Real-Time PCR set-Up
- Genetic test (DTC) service
- Experiment academy service
- Other customized service
< Related Product >
Electrophoresis Filter Lab supplies Instrument
• Agarose • DNA,RNA Purification
• Plasticware • Air Sterilizer
- Mini column
• DNA Staining solution - Cuvette, Glove, Grinder, • Digital PCR
- Nucleic acid - Hi lter Loop, Mask, Plate, Swab,
staining solution Tube • Centrifuge & Mixer
• DNA Ladder • Syringe filter • Deep Freezer
- 1kb, 100bp plus DNA ladder - CA, MCE, PES, Nylon, • Other Supplies • Electrophoresis System
• Protein Marker PVDF, PTFE-W, PTFE-O - Al cooling block,
- 10~180 kDa, 10~245 kDa Aluminum foil • Heating Block
Protein marker • Membrane filter • Homogenizer
• Pre-made Bu er - MCE, PES, Nylon,
- 50X TAE, 5X TBE, RNase free PVDF, PTFE • Transilluminator
water, 0.5M EDTA, 10X SDS PAGE
running buffer, Coomassie blue • Water Purification System
staining / destaining solution
15
BIOFACT CATALOGUE
DNA / RNA
Amplification
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
Description 18
Lyophilized PCR Enzyme
LyoFACT™ PCR Pre-Mix Lyophilisates 40
LyoFACT™ RT Pre-Mix Lyophilisates 42
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates 44
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (For Probe) 46
LyoFACT™ All in one PCR Pre-Mix Lyophilisates 48
Real-Time PCR Enzyme
Intercalating Dye BioFACT™ Real-Time PCR Series 56
SFCgreen® I 57
BioFACT™ Real-Time PCR Series (With SFCgreen® I) 59
BioFACT™ Real-Time PCR Series (With SYBR® Green I) 64
BioFACT™ Real-Time PCR Series (With EvaGreen™) 65
20X EvaGreen™ 66
BioFACT™ OneStep qRT-PCR Master Mix 67
FastFACT™ Real-Time PCR Master Mix 69
Probe BioFACT™ Multi-Star Real-Time PCR Master Mix 70
BioFACT™ OneStep Multi-Star qRT-PCR Series 72
FastFACT™ qPCR Master Mix 74
FastFACT™ qRT-PCR Master Mix 75
PCR Enzyme
Universal PCR BioFACT™ F-Star Taq DNA Polymerase (Hot Start) 78
(<3 kb, 5 kb) BioFACT™ Thumb Taq DNA Polymerase 80
BioFACT™ Taq DNA Polymerase 82
Gene Speci c PCR BioFACT™ H-Star Taq DNA Polymerase (Hot Start) 84
(<1 kb, 5 kb) BioFACT™ A-Star Taq DNA Polymerase (Hot Start) 86
Long PCR BioFACT™ Lamp Taq DNA Polymerase 88
(<40 kb, λ DNA 기준) BioFACT™ A-Star Lamp Taq DNA Polymerase (Hot Start) 90
High Fidelity PCR BioFACT™ Pfu DNA Polymerase 92
(<3 kb, <20 kb) BioFACT™ Lamp Pfu DNA Polymerase 94
Multiplex PCR (<1 kb BioFACT™ 2X Multi-Star PCR Series (Hot Start) 96
PCR Inhibition SimpleWay™ 2X PCR Master Mix III 98
RT & RT-PCR Enzyme
BioFACT™ RT Series 102
BioFACT™ OneStep RT-PCR Series 104
Isothermal Amplification
BioFACT™ Bst DNA Polymerase 108
Other Reagent
BioFACT™ Uracil DNA Glycosylase (UDG) 112
BioFACT™ dNTP and dNTP Set 113
5X Band Helper™ 114
BioFACT™ RNase Inhibitor 116
PCR Description
PCR (Polymerase Chain Reaction) is a principle where target regions of genomic DNA or cDNA are selected and the desired ones are ampli-
ed to make multiple copies. This technique undergoes the repetitive stage of the following PCR system. Then, in an in vitro setting, DNA
is ampli ed by more than 10 times minimally within several hours, which corresponds to its methods. Each PCR enzyme of the BIOFACT
5
is expressed in multiple amounts following the recombination of genes coding high-temperature DNA polymerase in E. coli. Thus, it is a
thermal resistant DNA polymerase that has been puri ed at a higher degree of purity.
Target gene Primer PCR Result analysis
selection design reaction (For electrophoresis
system)
PCR Workflow
PCR System (Thermal Cycling) Primer Design Guide
cycle #n It is recommended to design the primer as follows:
Chemistry selection Primer and 1. Length : 17 ~ 28 mer (Tm = 55 ~ 80 ℃) Data
qPCR
(DNA-binding Dyes probe design 2. Base composition : 50 - 60% (G+C) analysis
optimization
or Probe type) A
B 3. Setting of Tm value of forward primer to be similar to Tm value
C
D of reverse primer
E
4. Region to avoid : 1) G + C rich region
F
2) Repeated region and secondary structure
5’ 3’
3’ 5’
1’ Design Rule (= The Wallace rule)
1 temperature 1 1 1 *Annealing Temperature =Tm – (4 ~ 6 ℃)
Tm (℃) = 2(A+T) + 4(C+G)
3 3 3 3
2 2 2 2 (A+T) = Sum of A and T residues in the primer
0 cycle #1 cycle #2 cycle #3 cycle #4 (C+G) = Sum of C and G residues in the primer
PCR Additives ; 5X Band Helper™
1. Pre-denaturation T
2. Denaturation step ① 5X Band Helper™ is an innovative PCR additive which is highly suit-
Cycling able for use in all PCR enzymes of BIOFACT. It enables e cient am-
3. Primer annealing (Annealing step) ②
(20~40 cycle) pli cation and improved results can be obtained by adjusting of 5X
4. Extension step ③ Band Helper™ in the following cases.
5. Final extension step - Di cult templates which are GC-rich or have a high degree of
secondary structure
- Presence of non-speci c bands or products
* It is recommended to use a minimum amount of 5X Band Helper™ to avoid
the mutation in case of PCR enzymes that have delity features.
BIOFACT Product
F-Star Taq DNA Polymerase (p. 78), Thumb Taq DNA Polymerase (p. 80), Taq DNA Polymerase (p. 82)
Lamp Taq DNA Polymerase (p. 88), Pfu DNA Polymerase (p. 92), Lamp Pfu DNA Polymerase (p. 94)
18 BIOFACT CATALOGUE
DNA / RNA Amplification Description
Lyophilized Type PCR
Description
PCR Pre-Mix Lyophilized type is designed to increase storage stability by freeze-drying solution-typed PCR Pre-Mix. Its ready to use
format can be stored at room temperature for long-term storage while remaining structurally and chemically stable as the moisture
of it is decreased to less than 1%, unlike the wet dry formulation. In addition, PCR Pre-Mix Lyophilized type is fully reconstituted after
addition of water (rehydration). PCR Pre-Mix Lyophilized type o ers higher sensitivity as it can increase the amount of template than
solution-typed PCR Pre-Mix.
Feature
01 02 03 04
Stability Reproducibility Solubility Re-hydration
Stable storage Reproducible Lyophilized cake type Quick & perfect
more than 1 year at RT result data re hydration
05 06 07
Convenience Flexibility Sensitivity
Ready to use, 8-strip tube & 96-well High sensitivity &
pre-loaded type plate format ampli cation e ciency
BIOFACT Product
LyoFACT™ PCR Pre-Mix Lyophilisates (p. 40)
LyoFACT™ RT Pre-Mix Lyophilisates (p. 42)
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates (p. 44)
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (p. 46)
LyoFACT™ All in one PCR Pre-Mix Lyophilisate (p. 48)
19
BIOFACT CATALOGUE
Real-Time PCR Description
Real-TIme PCR
PCR
Primer
Result analysis
Target gene
Real-Time PCR (Quantitative PCR = qPCR) technique is the system that is identical to a general type of PCR, and it is ampli ed
selection
design
reaction
through a thermal cycling method. The amount and presence of the template (DNA or RNA) triggering this experimental reaction
(For electrophoresis
system)
should be con rmed, which is the objective. In case of standard PCR, the product completing the reaction is con rmed on electro-
phoresis. But in case of Real-time PCR, the increased amount on a real-time basis is con rmed in accurate numeric values (graph).
In an in vitro setting, DNA is ampli ed by more than 10 times, which corresponds to its methods. Each PCR enzyme of the BIOFACT
5
is expressed in multiple amounts following the recombination of genes coding high-temperature DNA polymerase in E. coli. Thus,
it is a thermal resistant DNA polymerase that has been puri ed at a higher degree of purity.
Exponential phase Non-
0.3
exponential
Plateau
phase
0.2 Chemistry selection
qPCR
Fluorescence (DNA-binding Dyes probe design optimization analysis
Primer and
Data
or Probe type)
0.1 C T value
Threshold line
Real-Time PCR Workflow
0
0 10 20 30 40
Cycle
Data Analysis
Absolute Quantification VS Relative Quantification qPCR
< Amplification curve >
Absolute quantification Relative quantification qPCR
Exponential phase Non-
0.3
exponential Amount of unknown
Plateau sample can be measured Target sample is quanti ed
phase Measurement compared standard curves based on the amount of
Fluorescence 0.2 method concentrations are known. other reference sample
with target sample whose
(control gene).
0.1 Ct value
Con rmation of viral copy Con rmation of di erences
Examples of use number depending on of gene expression depend-
Threshold line
infectious stages ing on the drug response
0
0 10 20 30 40
Cycle
It is available to compare the concentrations between samples using Ct value.
< Melting curve > <Examples of result data>
BIOFACT Company A
B. Normalized Melting curves Melting curves
100 F
F
F
75
Fluorescence 50 Double-stranded DNA Single-stranded DNA Derivative Reporter (-Rn’)
(dsDNA)
(ssDNA)
25
Company B Company C
0
76 79 82 Tm 88 91
Temperature [℃] Temperature [℃]
Applying the principle on the left gure, it enables to measure the reduced
amounts of dye according to changes in temperature. Through the result, it can
be con rmed whether target gene is speci cally ampli ed or not.
BIOFACT Product
Real-Time PCR Enzyme (p. 56), Multi-Star Real-Time PCR Enzyme (For Probe) (p. 70), OneStep Multi-Star qRT-PCR Enzyme (For Probe) (p. 72)
20 BIOFACT CATALOGUE
DNA / RNA Amplification Description
Hot Start PCR Description
Most of the DNA polymerases can extend misaligned primers to create primer-dimers or non-specific product before reaching
the denaturation temperature that has been set. Hot start PCR is a modi ed technique that is developed to avoid non-specific
amplification and limit primer-dimer formation during reaction preparation. By blocking polymerase activity prior to PCR cycling,
non-specific amplification can be reduced and target yield is increased. A variety of Hot start methods exist including chemical
modifications, wax barrier methods and inhibition by a Taq-directed antibody.
★ It is less likely to occur non-specific annealing while rising up to 95℃ because Hot start Taq is activated only at ★ temperature.
Denaturation
It is recommended to use Hot Start PCR method in the following cases :
• High specific PCR 95℃ 95℃ Extension
72℃
• PCR difficulty due to high GC content or secondary structure 55℃ 55℃ 72℃
• PCR difficulty due to non-specific product or primer-dimer
Annealing
• Multiplex PCR / Allele specific PCR
BIOFACT Hot Start PCR Enzymes
Chemical-mediated Hot Start Antibody-mediated Hot Start
- High specificity for targeted genes - Reliable ampli cation for template DNA containing low concentrations
- Pre-denaturation time : 2 ~ 15 mins. - Pre-denaturation time : 2 min.
BioFACT™ F-Star Taq BioFACT™ A-Star Taq DNA Polymerase
BioFACT™ H-Star Taq BioFACT™ A-Star Lamp Taq DNA Polymerase
chemical
SimpleWay™ Direct PCR
5’ 3’
BioFACT™ 2X Multi-Star PCR Series Heating &
PCR
BioFACT™ OneStep RT-PCR
3’ 5’
BioFACT™ Real-Time PCR Series
Chemical-mediated
Target DNA BioFACT TM H-Star Taq Primer dNTP
Chemical-mediated Hot Start Antibody-mediated Hot Start
chemical Anti-Taq antibody + Taq
5’ 3’ 3’
Heating & Heating &
PCR PCR
3’ 5’
3’ 5’
Chemical-mediated
Target DNA Primer dNTP Target DNA Anti-Taq antibody + Taq Primer dNTP
BioFACT TM H-Star Taq
Anti-Taq antibody + Taq
BIOFACT Product
3’
H-Star Taq DNA Polymerase (p. 84), A-StarTaq DNA Polymerase (p. 86), A-Star Lamp Taq DNA Polymerase (p. 90)
Heating &
PCR
2X Multi-Star PCR Series (p. 96)
3’ 5’
Target DNA Anti-Taq antibody + Taq Primer dNTP
21
BIOFACT CATALOGUE
Isothermal Amplification
Description
Isothermal ampli cation method is a simple process that rapidly and e ciently accumulates nucleic acid sequences at constant tem-
perature, compared with PCR. LAMP (Loop-mediated isothermal ampli cation) is a technique for rapid and speci c ampli cation of
DNA based on a sophisticated primer design that allows the detection of a target gene within 30-60 minutes, at 65°C. BioFACT™ Bst
DNA Polymerase applicable to LAMP PCR is isolated from Bacillus stearothermophilus and it has strand-displacing 5′-3′ polymerase
activity but no 5' to 3' exonuclease activity.
Application
• Loop-mediated isothermal ampli cation(LAMP) • Whole genome ampli cation
• Isothermal ampli cation • RT-LAMP
Comparison of PCR & LAMP (Loop-mediated isothermal amplification) PCR
PCR LAMP PCR
Amplification Denaturation (95°C), primer annealing (-60°C) Isothermal ampli cation (60-72°C)
method & polymerization (72°C)
Instrument PCR thermal cycler Heating block, water bath & dry oven
Assay reaction time Over 90 mins. 30-60 mins.
Result Electrophoresis required (Conventional PCR) Naked eye detection available in real time
BIOFACT Product
BioFACT™ Bst DNA Polymerase (p. 108)
22 BIOFACT CATALOGUE
DNA / RNA Amplification Description
Direct PCR Description
Direct PCR
Direct PCR is a method which samples are directly used for PCR ampli cation without extracting the additional genomic DNA
from various samples.
· It o ers fast, convenient PCR ampli cation and simple PCR protocol.
· There is no need for time-consuming and expensive DNA puri cation step by allowing PCR directly from unpuri ed samples.
· A multiple number of samples can be quickly ampli ed through the PCR ampli cation.
· Expensive instruments are not necessary other than the PCR thermal cycler.
· There is no need for time-consuming and expensive DNA puri cation step by allowing PCR directly from unpuri ed samples.
· It can be used with a wide variety of sample types
(Blood, saliva, oral epithelial cell and nail, SW411-100, bacterium and yeast, SW412-100).
Comparison of Experimental Step between General PCR & Direct PCR
1) General PCR sample gDNA isolation PCR
(30 min 이상)
gDNA isolation and PCR ampli cation sample gDNA isolation PCR
(30 min 이상)
over 30 min.
- gDNA with high-concentration required / long-term storage available
- Complex procedures / time-consuming
- Various instruments required (Centrifuge, heating block and etc.)
except thermal cycler
2) Direct PCR
Simply used for PCR ampli cation directly sample PCR
sample PCR
- Convenient PCR ampli cation from various samples
- No other instruments required except thermal cycler
- Inability to preserve genomic DNA
BIOFACT Product
SimpleWay™ 2X PCR Master Mix Ⅲ (p. 98)
23
BIOFACT CATALOGUE
UDG System Description
UDG System
PCR contamination remains an issue for laboratories performing detection of infectious agents and forensic procedures. The
single most important source of PCR product contamination is the generation of aerosols of PCR amplicons that is associated
with the post-PCR analysis. In addition to post-PCR contamination, the target template itself can be a source of contamination.
UDG System is mainly applied when performing PCR using a large number of samples such as PCR diagnosis or repeating the
same experiment. It is recommended to use in case reagents or equipment are contaminated by samples (product DNA) which
was used in the previous step.
1 Experiment 2 Experiment
st
nd
Sample A Sample B
C A G T C T C G T A
Sample B + UDG + dNTP(U) mix
G T C A G A G C A T
Contamination
dNTP(U) mix UDG
PCR (50℃, 3 min)
(dATP, dCTP, dGTP, dUTP) activation
C A G U C
G U C A G
PCR UDG inactivation
(PCR rst step)
1) PCR product from contaminated sample A 2) PCR product from sample B
U C G U A
C A G C A U U C G U A
A G C A U
U C G U A
C A G A G C A U
U C G U A
G C A G A G C A U U C G U A
U C G U A A G C A U
A G C A U
PCR product is degraded when U is cut Properly ampli ed DNA
Sample A Sample B
BIOFACT Product
Uracil DNA Glycosylase (UDG) (p. 112)
24 BIOFACT CATALOGUE
DNA / RNA Amplification Description
Fast PCR Description
Fast PCR
The COVID-19 pandemic caused by the SARS-CoV-2 virus began late 2019 and has now spread to virtually every country in the
world. Prompt identi cation of individuals shedding SARS-CoV-2 to prevent further transmission is integral to an e ective public
health response, a measure predicated upon the widespread availability of rapid diagnostics for SARS-CoV-2. quantitative poly-
merase chain reaction (qPCR) is the current gold standard method for detecting SARS-CoV-2, as it o ers exceptional sensitivity,
speci city and high-throughput platform.
BIOFACT Fast qPCR reaction takes approximately 40 minutes to completely detect DNA or RNA of less than 500bp by extremely
shortening the denaturation, annealing, and extension time which takes more than 2 hours in normal qPCR reaction.
Protocol Comparison between Standard qPCR & Fast PCR Reaction
Standard qPCR protocol Fast PCR protocol
95℃ for 5 min.
95℃ for 2 min.
95℃ for 20 sec.
95℃ for 5 sec.
60℃ for 40 sec. 30 cycle 30 cycle
72℃ for 1 min. 68℃ for 30 sec.
72℃ for 5 min.
Lead time 40min.
Lead time 2hr.
BIOFACT Product
FastFACT™ 2X Real-Time PCR Master Mix (For Intercalating Dye) (p. 69)
FastFACT™ 2X qPCR Master Mix (For Probe) (p. 74)
FastFACT™ 2X qRT-PCR Master Mix (For Probe) (p. 75)
25
BIOFACT CATALOGUE
DNA-Free System
Description
DNA-Free System
DNA-Free System o ers minimized risk of DNA contamination in the enzyme while maintaining the performance speci cations associated Hot Start
technology. DNA-free system uses BIOFACT DNA-Free Taq Polymerase which is highly puri ed recombinant enzyme that removes 99.9% of bacterial
host (E. Coli) DNA contamination through our own puri cation technology. It is especially useful for 16S and 23S rDNA gene ampli cation, sequencing
and cloning as well as for all regular applications, including PCR and primer extension. BIOFACT DNA-Free Taq Polymerase is also highly suitable for
molecular diagnostics through its high-quality.
▶ High-purity recombinant thermostable Taq polymerase that removes 99.9% of bacterial host (E. Coli) DNA contamination
▶ Diagnosis of pathogenic bacteria such as sepsis or food poisoning (Foodborne illness)
▶ 16S and 23S rDNA gene amplification using E.Coli primers
E. coli
Host (E. coil)
genomic DNA
Taq DNA polymerase
Common
Taq DNA polymerase
DNA-Free System
Small amounts of E.Coli DNA are detected
Host genomic DNA are removed completely
NTC Positive
MW 1 2 3 4
E. Coli 16S rDNA ampli cation
NTC : Non-template control
Positive : 3.7pg of E. coli genomic DNA added
1 : NTC, 1’ PCR 2 : NTC, 2’ PCR
3 : Positive, 1’ PCR 4 : Positive, 2’ PCR
BIOFACT Product
Launch planned
26 BIOFACT CATALOGUE
DNA / RNA Amplification Description
27
BIOFACT CATALOGUE
BIOFACT
PCR Enzyme Map
Lyophilized Real-Time PCR Conventional Reverse- Isothermal Other
Transcription
PCR (qPCR) PCR (RT-PCR) Ampli cation Reagents
LyoFACT™ PCR Pre-Mix Intercalating Dye BioFACT™ F-Star Taq
Lyophilisates BioFACT™ Real-Time PCR Universal BioFACT™ Thumb Taq BioFACT™ RT Series BioFACT™ Bst DNA BioFACT™ Uracil DNA
(For SFCgreen® I) PCR BioFACT™ Taq Polymerase Glycosylase (UDG)
LyoFACT™ RT Pre-Mix
Lyophilisates BioFACT™ Real-Time PCR
(For SYBR® Green I) Gene BioFACT™ H-Star Taq BioFACT™ dNTP
LyoFACT™ OneStep RT-PCR Speci c PCR BioFACT™ A-Star Taq BioFACT™ OneStep and dNTP Set
Pre-Mix Lyophilisates BioFACT™ Real-Time PCR BioFACT™ 2X Multi-Star RT- PCR Series
(For EvaGreen™) PCR Series 5X Band Helper™
LyoFACT™ OneStep qRT-PCR
Pre-Mix Lyophilisates FastFACT™ 2X Real-Time
PCR Master Mix BioFACT™ Lamp Taq BioFACT™ RNase Inhibitor
LyoFACT™ All in one PCR Long PCR
Pre-Mix, Lyophilisate Probe BioFACT™ A-Star Lamp Taq
BioFACT™ Multi-Star
Real-Time PCR Series BioFACT™ Pfu
High
BioFACT™ OneStep Fidelity PCR BioFACT™ Lamp Pfu
Multi-Star qRT-PCR Series
PCR Inhibition SimpleWay™
FastFACT™ 2X 2X PCR Master Mix III
qPCR Master Mix
(Launch planned)
BioFACT™ Fast PCR Enzyme (출시예정)
FastFACT™ 2X
qRT-PCR Master Mix BioFACT™ Host DNA-Free PCR Enzyme (출시예정)
(Launch planned)
28 BIOFACT CATALOGUE
DNA / RNA Amplification Description
Lyophilized Real-Time PCR Conventional Reverse- Isothermal Other
Transcription
PCR (qPCR) PCR (RT-PCR) Ampli cation Reagents
LyoFACT™ PCR Pre-Mix Intercalating Dye BioFACT™ F-Star Taq
Lyophilisates BioFACT™ Real-Time PCR Universal BioFACT™ Thumb Taq BioFACT™ RT Series BioFACT™ Bst DNA BioFACT™ Uracil DNA
(For SFCgreen® I) PCR BioFACT™ Taq Polymerase Glycosylase (UDG)
LyoFACT™ RT Pre-Mix
Lyophilisates BioFACT™ Real-Time PCR
(For SYBR® Green I) Gene BioFACT™ H-Star Taq BioFACT™ dNTP
LyoFACT™ OneStep RT-PCR Speci c PCR BioFACT™ A-Star Taq BioFACT™ OneStep and dNTP Set
Pre-Mix Lyophilisates BioFACT™ Real-Time PCR BioFACT™ 2X Multi-Star RT- PCR Series
(For EvaGreen™) PCR Series 5X Band Helper™
LyoFACT™ OneStep qRT-PCR
Pre-Mix Lyophilisates FastFACT™ 2X Real-Time
PCR Master Mix BioFACT™ Lamp Taq BioFACT™ RNase Inhibitor
LyoFACT™ All in one PCR Long PCR
Pre-Mix, Lyophilisate Probe BioFACT™ A-Star Lamp Taq
BioFACT™ Multi-Star
Real-Time PCR Series BioFACT™ Pfu
High
BioFACT™ OneStep Fidelity PCR BioFACT™ Lamp Pfu
Multi-Star qRT-PCR Series
PCR Inhibition SimpleWay™
FastFACT™ 2X 2X PCR Master Mix III
qPCR Master Mix
BioFACT™ Fast PCR Enzyme (출시예정)
FastFACT™ 2X
qRT-PCR Master Mix BioFACT™ Host DNA-Free PCR Enzyme (출시예정)
29
BIOFACT CATALOGUE
Stability Test
BIOFACT PCR Enzyme Quality Control System
Stability Test on Various Temperature Ranges
<BioFACT™ H-Star Taq> <BioFACT™ Real-Time PCR Enzyme>
A B C D E F A B C D E F
-20℃ -20℃ -20℃ -20℃
72℃ 15 min 95℃ 15 min 72℃ 15 min 95℃ 15 min
72℃ 30 min 95℃ 30 min 72℃ 30 min 95℃ 30 min
72℃ 1 hr 95℃ 1 hr 72℃ 1 hr 95℃ 1 hr
72℃ 2 hr 95℃ 2 hr 72℃ 2 hr 95℃ 2 hr
72℃ 4 hr 95℃ 4 hr 72℃ 4 hr 95℃ 4 hr
A : 50 ng 50 ng
72℃ 8 hr 72℃ 8 hr
B : 10 ng 10 ng
C : 2 ng 2 ng
72℃ 12 hr 72℃ 12 hr
D : 0.4 ng 0.8 ng
E : 0.08 ng
72℃ 24 hr 72℃ 24 hr
F : 0.016 ng
Stability Test on Various Periods
<BioFACT™ Taq> <BioFACT™ F-Star Taq> <BioFACT™ Multi-Star PCR Enzyme>
-
20℃ 4℃ 20℃ 4℃ 20℃ 4℃
-
-
A B C D A B C D A B C D A B C D A B C D A B C D
1 week 1 week
1 week
4 week 4 week
10 week 10 week
16 week 16 week 4 week
30 week 30 week
56 week 56 week
10 week
80 week 80 week
p53 primer p53 primer
A : 2.5 U B : 1.25 U C : 0.62 U D : 0.31 U A : 2.5 U B : 1.25 U C : 0.62 U D : 0.31 U
<BioFACT™ Pfu> <BioFACT™ RTase> 16 week
-
20℃ 4℃ 20℃ 4℃
-
A B C D A B C D A B C D A B C D
1 week 1 week 30 week
4 week 4 week
10 week 10 week
56 week
16 week 16 week
30 week 30 week
56 week 56 week 80 week
80 week 80 week
GLO primer Human Total RNA 10 ng (Oligo dT(18)), β-Actin Rice primer
A : 2.5 U B : 1.25 U C : 0.62 U D : 0.31 U A : cDNA 5 0 B : cDNA 5 -1 C : cDNA 5 -2 D : cDNA 5 3 A : 2.5 U B : 1.25 U C : 0.62 U D : 0.31 U
• Long-term test : -20℃ (Expiration date 2 Years and 3 months, 821.25days)
• Acceleration test : 4℃ *TAA(Accelerated aging temp. / Desired Temp.) : 4℃ *TRT(Ambient Temp. / Preservation Temp.) : -20℃ *AAF(AAF = Q10 [(TAA-TRT)/10] ) : 5.27
*AAT : 821.25/5.27 = 155.83 days = 22.27 weeks (Accelerated aging conditions for expiration date, 2 years and 3 months)
30 BIOFACT CATALOGUE
DNA / RNA Amplification Description
Efficiency Test
BIOFACT PCR Enzyme Quality Control System
Comparison Test of LOD
<Enzyme LOD test> <Template LOD test>
ApoE (0.9 kb) APEX (1 kb)
M A B C D E M M A B C D E F M M A B C D E F M
BIOFACT BIOFACT
Company A
Company A
Company B
Company B
Company C
Company C
Company D
Target : Human genomic DNA 10 ng PCR Condition : 60 °C, 35 cycles Target : Human genomic DNA PCR Condition : 60 °C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 2.5U B : 1.25U C : 0.625U D : 0.313U E : 0.156U A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng
Comparison Test between BIOFACT PCR Enzyme and Other Companies' PCR Enzymes /
Comparison Test between BioFACT™ Taq and BioFACT™ A-Star Taq
BIOFACT Company A Company B Company C Company D
M A B C D E M A B C D E M A B C D E M A B C D E M A B C D E M
Target : Human genomic DNA 50 ng
Gene : β-Globin
PCR Condition : 60 °C, 35 cycles
M : 1kb DNA Ladder
A : 0.5 kb B : 1 kb
C : 2 kb D : 3.6 kb
E : 5 kb
NF-κB (1.2 kb) TNFR1
Taq A-Star Taq Taq A-Star Taq
M A B C D E F M A B C D E F M A B C D E F M A B C D E F
Target : Human genomic DNA
PCR Condition : 60℃, 35 cycles
M : 1kb DNA Ladder
A : 50 ng B : 10 ng C : 2 ng
D : 0.4 ng E : 0.08 ng F : 0.016 ng
BIOFACT Company A Company B Company C
M A B C D E F G M M A B C D E F G M M A B C D E F G M M A B C D E F G M
Template : Lambda DNA
PCR Condition : AT 68℃, 30 Cycles
M : 1kb DNA Ladder
A : 1 kb B : 3.6 kb C : 5 kb D : 10 kb
E : 14 kb F : 17 kb G : 22 kb
31
BIOFACT CATALOGUE
BIOFACT PCR Enzyme
Product Types
Standard Type Master Mix Type Pre-Mix Type
Standard Type : DNA polymerase, dNTP, PCR bu er and etc. in each tube
Master Mix Type : Ready to use master mix in 1.5 ㎖ tube
Pre-Mix Type : Ready to use master mix in 8-strip tube (0.2 ㎖)
Type
Classification Product Master Mix Pre-Mix
Standard Type Type Pre-Mix Type (Lyophilisates)
BioFACT™ Real-Time PCR Enzyme ○
BioFACT™ Real-Time PCR (For SFCgreen® I) ○ ○
BioFACT™ Real-Time PCR (For SYBR® Green I) ○ ○
BioFACT™ Real-Time PCR (For EvaGreen™ ) ○ ○
Real-Time
BioFACT™ Multi-Star Real-Time PCR (For Probe) ○
BioFACT™ OneStep Multi-Star qRT PCR (For Probe) ○ ○
FastFACT™ 2X qPCR Master Mix ○
FastFACT™ 2X qRT-PCR Master Mix ○
BioFACT™ F-Star Taq ○ ○ ○
Universal BioFACT™ Thumb Taq ○ ○ ○
BioFACT™ Taq ○ ○ ○ ○
BioFACT™ H-Star Taq ○ ○ ○
Gene Specific
BioFACT™ A-Star Taq ○
BioFACT™ Lamp Taq ○ ○ ○
Long
BioFACT™ A-Star Lamp Taq ○
BioFACT™ Pfu ○ ○ ○
High Fidelity
BioFACT™ Lamp Pfu ○
Multiplex BioFACT™ 2X Multi-Star PCR Series ○ ○ ○
BioFACT™ RT Series ○ ○ ○
cDNA Synthesis
BioFACT™ OneStep RT-PCR Series ○ ○ ○ ○
Isothermal BioFACT™ Bst DNA Polymerase ○
32 BIOFACT CATALOGUE
DNA / RNA Amplification Description
BIOFACT DNA Polymerase
Selection Guide Ⅰ
BioFACT™ Real-Time PCR BioFACT™ F-Star Taq BioFACT™ Thumb Taq BioFACT™ Taq BioFACT™ H-Star Taq BioFACT™ A-Star Taq BioFACT™ Lamp Taq BioFACT™ A-Star Lamp Taq BioFACT™ Pfu BioFACT™ Lamp Pfu BioFACT™ 2X Multi-Star PCR BioFACT™ RT BioFACT™ OneStep RT-PCR
Universal PCR ▒ ▒ ▒ ▒ ▒ ▒ ▒
Hot Start PCR ▒ ▒ ▒ ▒ ▒ ▒ ▒
Long PCR ▒ ▒ ▒
Inverse PCR ▒ ▒
Multiplex PCR ▒ ▒
Real-Time PCR ▒ ▒ ▒
Allele Specific PCR ▒ ▒
Specification
Methylation Specific PCR ▒ ▒
Reverse Transcription ▒ ▒
RT-PCR ▒ ▒ ▒
High Fidelity PCR ▒ ▒
Colony PCR ▒ ▒ ▒ ▒ ▒ ▒ ▒
Genotyping ▒ ▒ ▒ ▒ ▒ ▒
TA Cloning ▒ ▒ ▒ ▒ ▒ ▒ ▒ ▒ ▒ ▒
Blunt end Cloning ▒ ▒
General Sequencing ▒ ▒ ▒ ▒ ▒ ▒ ▒ ▒ ▒ ▒ ▒ ▒
Application
Site-directed Mutagenesis ▒ ▒
Gene Expression ▒ ▒
UDG System ▒ ▒ ▒ ▒ ▒ ▒
33
BIOFACT CATALOGUE
BIOFACT DNA Polymerase
Selection GuideⅡ
3' → 5'
5' → 3'
cDNA
Product Exonuclease Exonuclease A-tailing Product Extension Yield Specificity Sensitivity Synthesis Isothermal
Time
Size
(fidelity)
BioFACT™ Real-Time PCR Yes No Yes < 1 kb 1 min / kb ＊＊ ＊＊＊＊＊ ＊＊
BioFACT™ F-Star Taq Yes No Yes < 3 kb 1 min / kb ＊＊＊ ＊＊＊＊ ＊＊＊＊
BioFACT™ Thumb Taq Yes No Yes < 5 kb 1 min / kb ＊＊＊＊＊ ＊＊ ＊＊＊＊
BioFACT™ Taq Yes No Yes < 5 kb 1 min / kb ＊＊＊＊ ＊＊ ＊＊＊＊
BioFACT™ H-Star Taq Yes No Yes < 1 kb 1 min / kb ＊＊ ＊＊＊＊＊ ＊＊
BioFACT™ A-Star Taq Yes No Yes < 5 kb 1 min / kb ＊＊＊＊ ＊＊＊ ＊＊＊＊
BioFACT™ Lamp Taq Yes Yes w Yes < 40 kb 1 min / kb ＊＊＊＊＊ ＊＊＊ ＊＊＊
(λ DNA기준)
BioFACT™ A-Star Lamp Taq Yes Yes w Yes < 40 kb 1 min / kb ＊＊＊＊ ＊＊＊ ＊＊＊＊
(λ DNA기준)
BioFACT™ Pfu No Yes No < 3 kb 2 min / kb ＊＊＊ ＊＊ ＊＊
BioFACT™ Lamp Pfu No Yes No < 20 kb 0.5 min / kb ＊ ＊ ＊
BioFACT™ 2X Multi-Star PCR Yes No Yes < 1 kb 1 min / kb ＊＊ ＊＊＊＊＊ ＊＊
BioFACT™ RT Yes
BioFACT™ OneStep RT-PCR Yes No Yes < 1 kb 1 min / kb ＊＊ ＊＊＊＊＊ ＊＊ Yes
BioFACT™ Bst No No Yes
- Yes : Weak 3'→ 5' exonuclease ( delity) activity
W
- Yield : PCR ampli cation e ciency, amounts of end-product after PCR ampli cation
- Extension Time : Ampli cation length and extension time are di erent depending on the speci c characteristics of each PCR enzyme
- Speci city : Target speci city, ampli cation level for target product without non-speci c bands
- Sensitivity : Sensitivity for template DNA concentration, PCR ampli cation level according to the template DNA concentration
34 BIOFACT CATALOGUE
DNA / RNA Amplification Description
BIOFACT DNA Polymerase
Selection Guide Ⅲ
Amplification of blunt-end fragment with high fidelity
Fidelity
• 3' → 5' exonuclease activity (fidelity) : Yes
Pfu DNA Polymerase
Lamp Pfu DNA Polymerase • 12 times higher PCR fidelity over general Taq DNA Polymerase
6
(Error rate : 1-2 bp error / 10 bp
Highly specific amplification of less than 1 kb size target DNA
Specificity • High PCR specificity, avoiding extension of non-specific annealed
primers or primer-dimers
H-Star Taq DNA Polymerase
2X Multi-Star PCR Series • Efficient amplification of difficult templates caused by high G+C content
or complex structure
Hot Start PCR enzyme for high sensitivity & high efficiency
Sensitivity
• Effective amplification of low-copy-number target DNA
A-Star Taq DNA Polymerase
A-Star Lamp Taq DNA Polymerase • High suitable for amplification reactions involving complex
gDNA or cDNA templates
Productivity High performance enzyme with improved sensitivity, specificity and yields
Lamp Taq DNA Polymerase • Reliable amplification of complex amplicon targets with a robust yield
Thumb Taq DNA Polymerase
A-Star Lamp Taq DNA Polymerase • Versatile, easy-to-use enzyme for various applications
Product Size Long range PCR amplified up to 15 kb and beyond
Lamp Taq DNA Polymerase • Amplification of DNA lengths that cannot typically be amplified using
A-Star Lamp Taq DNA Polymerase routine PCR methods
Lamp Pfu DNA Polymerase • Accurate PCR enzyme with low error rate & high productivity
35
BIOFACT CATALOGUE
BIOFACT DNA Polymerase
Selection Guide Ⅳ
BIOFACT DNA Polymerase Series
Specification Products Similar products from other companies
Premium BioFACT™ F-Star Taq -
BioFACT™ Thumb Taq
Universal ABI AmpliTaq, Ampliqon Taq, Beamsbio Taq-PCR Master Mix, Bioneer AccuPower®
PCR Taq, Bioron Top-Taq, Cosmogenetech G-Taq, Thermo Scientific Dream Taq, Elpis
General bio rTaq / HiPi /HiPi Eco Taq, Enzynomics nTaq, Fermentas Taq, GenetBio Prime
Taq, Intron i-Taq / i-Taq plus, Invitrogen T , Promega Go Taq, Qiagen Taq / TopTaq,
BioFACT™ Taq
Roche Taq, Takara Taq
Qiagen HotStartTaq, ABI AmpliTaq Gold, Roche Faststar Taq, Promega Go-Taq
Chemical- BioFACT™ H-Star Taq Hotstart, Cosmogenetech Hotstart-Taq, Fermentas Maxima Hot start Taq,
mediated
Truestar Hot start Taq
Gene
Specific
PCR Bioron SuperHotTaq DNA Polymerase, Intron i-StarTaq™ GH DNA Polymerase,
Antibody- BioFACT™ A-Star Taq KAPA KAPA Taq HotStart PCR Kit, Invitrogen Platinum® Taq DNA Polymerase,
mediated Promega GoTaq® Hot Start Polymerase, GoTaq® G2 Hot Start Polymerase, TaKaRa
TaKaRa Taq HS
Takara LA-Taq, Elpis biotech Hipi super Taq / Hipi plus Taq, Invitrogen Platinum
General BioFACT™ Lamp Taq
Taq High Fidelity, Fermentas Long PCR enzyme mix
Long PCR Intron i-StarMAX™ GH DNA Polymerase, KAPA KAPA Long Range HotStart DNA
Polymerase, Invitrogen Platinum® Taq DNA Polymerase High Fidelity, AccuPrimer™
Antibody- BioFACT™ A-Star Taq DNA Polymerase High Fidelity, Elongase® Enzyme Mix, Promega GoTaq® Long
mediated Lamp Taq
PCR Master Mix, Solis biodyne 5x HOT FIREPol® Blend Master Mix, TaKaRa TaKaRa
LA Taq HS, TaKaRa Ex Taq HS, Toyobo Blend Taq® –Plus-
Bioneer Pfu, Takara Pyrobest, PrimeSTAR HS, Roche Pwo SuperYield,
General BioFACT™ Pfu
Invitrogen AccuPrime Pfx, Fermentas Pfu, Phusion High delity
High
Fidelity
PCR
Long BioFACT™ Lamp Pfu Bioneer AccuPower® Pfu, Enzynomics nPfu-Forte
Multiplex General BioFACT™ Multi-Star PCR Qiagen Multiplex PCR kit, Intron i-D PCR, Bioneer Accupower Multiplex PCR,
PCR Komabiotech Multiplex PCR
NEB Bst 2.0, Bst 3.0, Bst DNA Polymerase, Large Fragment, Nanohelix HelixAmp™
Isothermal
amplification General BioFACT™ Bst Bst, Enzynomics Bst DNA Polymerase (Large Fragment), ELPIS BIOTECH Bst DNA
polymerase, LF exo-, Bst DNA Polymerase V2
36 BIOFACT CATALOGUE
DNA / RNA Amplification Description
37
BIOFACT CATALOGUE
DNA / RNA
Amplification
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
Description 18
Lyophilized PCR Enzyme
LyoFACT™ PCR Pre-Mix Lyophilisates 40
LyoFACT™ RT Pre-Mix Lyophilisates 42
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates 44
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (For Probe) 46
LyoFACT™ All in one PCR Pre-Mix Lyophilisates 48
Real-Time PCR Enzyme
Intercalating Dye BioFACT™ Real-Time PCR Series 56
SFCgreen® I 57
BioFACT™ Real-Time PCR Series (With SFCgreen® I) 59
BioFACT™ Real-Time PCR Series (With SYBR® Green I) 64
BioFACT™ Real-Time PCR Series (With EvaGreen™) 65
20X EvaGreen™ 66
BioFACT™ OneStep qRT-PCR Master Mix 67
FastFACT™ Real-Time PCR Master Mix 69
Probe BioFACT™ Multi-Star Real-Time PCR Master Mix 70
BioFACT™ OneStep Multi-Star qRT-PCR Series 72
FastFACT™ qPCR Master Mix 74
FastFACT™ qRT-PCR Master Mix 75
PCR Enzyme
Universal PCR BioFACT™ F-Star Taq DNA Polymerase (Hot Start) 78
(<3 kb, 5 kb) BioFACT™ Thumb Taq DNA Polymerase 80
BioFACT™ Taq DNA Polymerase 82
Gene Speci c PCR BioFACT™ H-Star Taq DNA Polymerase (Hot Start) 84
(<1 kb, 5 kb) BioFACT™ A-Star Taq DNA Polymerase (Hot Start) 86
Long PCR BioFACT™ Lamp Taq DNA Polymerase 88
(<40 kb, λ DNA 기준) BioFACT™ A-Star Lamp Taq DNA Polymerase (Hot Start) 90
High Fidelity PCR BioFACT™ Pfu DNA Polymerase 92
(<3 kb, <20 kb) BioFACT™ Lamp Pfu DNA Polymerase 94
Multiplex PCR (<1 kb BioFACT™ 2X Multi-Star PCR Series (Hot Start) 96
PCR Inhibition SimpleWay™ 2X PCR Master Mix III 98
RT & RT-PCR Enzyme
BioFACT™ RT Series 102
BioFACT™ OneStep RT-PCR Series 104
Isothermal Amplification
BioFACT™ Bst DNA Polymerase 108
Other Reagent
BioFACT™ Uracil DNA Glycosylase (UDG) 112
BioFACT™ dNTP and dNTP Set 113
5X Band Helper™ 114
BioFACT™ RNase Inhibitor 116
LyoFACT™ PCR Pre-Mix
Lyophilized Typed PCR Pre-Mix
LyoFACT™ PCR Pre-Mix is designed to increase storage stability by freeze-drying solution-typed PCR Pre-Mix. Its ready to use format
can be stored at room temperature for long-term storage while remaining structurally and chemically stable as the moisture of it is
decreased to less than 1%, unlike the wet dry formulation. In addition, it is fully reconstituted after addition of water (rehydration).
Feature
• Source : Thermus aquaticus • Hot Start activity : Taq (No), Multi-Star (Yes(chemical-mediated))
6
• 5' → 3' exonuclease activity : Yes • 12-13 bp error / 10 bp
• 3' → 5' exonuclease activity (Fidelity) : No • A-tailing : Yes
• Amplification size : Taq < 5kb, Multi-Star <1kb • Amplification of more than one target in a single reaction (Multi-Star)
Application
- Taq - Multi-Star
• Routine PCR • Multiplex PCR ( ~ 13 bands in a parallel)
• PCR screening • Allele specific PCR ( ~ 21 bands in a parallel)
• TA cloning • Methylation specific PCR
• Colony PCR • SNP detection
• General sequencing
• TA cloning
• SSR / MS
• UDG system
QC DATA
- Stability test on various periods (Changes in cake shape)
기간별 안정성 테스트 [제조사별 Cake 형태 변화]
BIOFACT Company A Company B
타사 (B)
타사 (A)
BIOFACT
0 week
After
20 weeks
* 보관 온도 : 상온 * Cake 형태가 보관 후에 변형되면 제품의 성능이 저하될 우려가 있습니다.(타사 A : cake size 감소, 타사 B : 수분 생성)
* Storage temp. : 4 ℃
* There is a risk of performance degradation in case the shape of the cake is changed.
(Company A: cake size is decreased, Company B: moisture is created.)
40 BIOFACT CATALOGUE
DNA / RNA Amplification Lyophilized PCR Enzyme
- Comparison of Stability test according to various periods between LyoFACT™ PCR Pre-Mix and other lyophilized typed PCR Mix
LyoFACT™ PCR Pre-Mix <LyoFACT™ Taq>
0 week After 20 week
Company A BIOFACT [ST501-096] Company A BIOFACT [ST501-096]
M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4
Storage temp. : ST501-096 (4°C) Genomic DNA Conc. : 1 : 50ng 2 : 10ng 3 : 2ng 4: 0.4ng M : BioFACT™ 1 Kb Plus DNA Ladder
Target size : 1.1Kb 1.5% Agarose gel / 5㎕ loading
<LyoFACT™ Multi-Star>
0 week After 20 week
Company A BIOFACT [MS501-096] Company A BIOFACT [MS501-096]
M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4
Storage temp. : ST501-096 (4°C) Genomic DNA Conc. : 1 : 50ng 2 : 10ng 3 : 2ng 4: 0.4ng M : BioFACT™ 1 Kb Plus DNA Ladder
8-plex reaction(100~600bp) 1.5% Agarose gel / 5㎕ loading
Product Information
Pre-Mix Type Pre-Mix Type
LyoFACT™ Taq PCR Pre-Mix Lyophilisates, with dye LyoFACT™ Multi-Star PCR Pre-Mix Lyophilisates, with dye
[ST501-096] [MS501-096]
▶Contents ▶Expiration date ▶Contents ▶Expiration date
LyoFACT™ Taq PCR Pre-Mix 1 Year at -20 ± 5℃ LyoFACT™ Multi-Star PCR 1 Year at -20 ± 5℃
Lyophilisates, with dye Pre-Mix Lyophilisates, with dye
[96 Tubes] [96 Tubes]
Maker Cat.No. Product Size
ST501-096 LyoFACT™ Taq PCR Pre-Mix Lyophilisates, with dye 96 Tubes (8 strip × 12 ea)
BIOFACT
MS501-096 LyoFACT™ Multi-Star PCR Pre-Mix Lyophilisates, with dye 96 Tubes (8 strip × 12 ea)
41
BIOFACT CATALOGUE
LyoFACT™ RT Pre-Mix
Lyophilized Typed RT Pre-Mix Including Primers
LyoFACT™ RT Pre-Mix is based on recombinant type of M-MLV reverse transcriptase(RNase H ) expressed in E. coli which has greater
-
rst-strand cDNA yields than existing wild type of reverse transcriptase due to inhibition of RNA's secondary structure by its thermo-
stable characteristic at 50℃ for activation. LyoFACT™ RT Pre-Mix is a lyophilized type designed to increase storage stability and it is
supplied as a ready-to-use, 2X concentrated Pre-Mix which includes an optimized mixture of all the reagents such as RTase, RNase
inhibitor, DTT and dNTPs in a 0.2 ㎖ 8-strip tube. In addition, Oligo dT (dT18) or Random Oligo (dN9) are included on the kit and it
can be used by adding template RNA & RNase free water only. It o ers higher sensitivity as it can increase the amount of template
than solution-typed PCR Pre-Mix.
Feature
• Thermostable and RNase H variant of M-MLV RTase (RNase H ) • Synthesis primer : random primer, oligo-d(T), gene specific primer
-
• Synthesize cDNA at 42℃ – 50℃ • Inhibition of RNA's secondary structure
• Especially the highest activity at 50℃ • Oligo(dT)18 and(or) random hexamer primers provided
• Synthesis of long length cDNA (＜14 kb)
Application
• First-strand cDNA synthesis • Real-Time PCR
• RT-PCR • Primer extension analysis
QC DATA
- Comparison of efficiency according to various sizes of - Comparison of cDNA synthesis efficiency on various RNA
cDNA synthesized by LyoFACT™ RT Pre-Mix concentrations between LyoFACT™ RT Pre-Mix and other
companies lyophilisates
(A) (B)
Total RNA
Conc. ▶ 100 ng 20 ng 4 ng 0.8 ng 100 ng 20 ng 4 ng 0.8 ng
M 1 2 3 4 5 M M A B C D A B C D A B C D A B C D M M A B C D A B C D A B C D A B C D M
BIOFACT
Company A
Company B
Company C
Template : Human total RNA 1㎍ Target : Human total RNA, β-Actin (1 kb) gene
RT Kit : BR546-096 [LyoFACT™ RT Pre-Mix(dT18 Plus)] PCR condition : 60℃, 35 cycles
Synthesize cDNA at 50℃ RT Kit : (A) LyoFACT™ RT Pre-Mix(dT18 Plus)[BR546-096]
1% Agarose gel / 5 ㎕ loading (B) LyoFACT™ RT Pre-Mix(dN9 Plus)[BR547-096]
M : DNA Ladder 1 : 0.7 kb 2 : 1 kb 3 : 3.6 kb 4 : 5 kb 5 : 10 kb 1% Agarose gel / 5 ㎕ loading
-1
-2
-3
M : DNA Ladder A : cDNA 5 B : cDNA 5 C : cDNA 5 D : cDNA 5
0
42 BIOFACT CATALOGUE
DNA / RNA Amplification Lyophilized PCR Enzyme
- Comparison of stability test of LyoFACT™ RT Pre-Mix over a long period of time
LyoFACT™ RT Pre-Mix M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4
2 month
6 month
0 month
12 month
(A)
(B)
(C)
Target : Human total RNA, 4-plex PCR PCR condition : 60℃, 40 cycles 2% Agarose gel / 7㎕ loading
Kit : (A) LyoFACT™ RT Pre-Mix(dT18 Plus)[BR546-096] (B) LyoFACT™ RT Pre-Mix(dN9 Plus)[BR547-096] (C) LyoFACT™ RT Pre-Mix(dT18/dN9 Plus)[BR548-096]
M : DNA Ladder 1 : RNA 100 ng 2 : RNA 20 ng 3 : RNA 4 ng 4 : RNA 0.8 ng
Product Information
Pre-Mix Type
LyoFACT™ RT Pre-Mix (dT18 Plus) Lyophilisates [BR546-096]
▶Contents
LyoFACT™ RT Pre-Mix (dT18 Plus) Lyophilisates [96 Tubes]
▶Expiration date
1 Year at -20 ± 5℃
Pre-Mix Type Pre-Mix Type
LyoFACT™ RT Pre-Mix (dN9 Plus) Lyophilisates LyoFACT™ RT Pre-Mix (dT18/dN9 Plus) Lyophilisates
[BR547-096] [BR548-096]
▶Contents ▶Contents
LyoFACT™ RT Pre-Mix (dN9 Plus) Lyophilisates [96 Tubes] LyoFACT™ RT Pre-Mix (dT18/dN9 Plus) Lyophilisates [96 Tubes]
▶Expiration date ▶Expiration date
1 Year at -20 ± 5℃ 1 Year at -20 ± 5℃
Maker Cat.No. Product Size
BR546-096 LyoFACT™ RT Pre-Mix (dT18 Plus) Lyophilisates 96 Tubes (8 strip × 12 ea)
BIOFACT BR547-096 LyoFACT™ RT Pre-Mix (dN9 Plus) Lyophilisates 96 Tubes (8 strip × 12 ea)
BR548-096 LyoFACT™ RT Pre-Mix (dT18/dN9 Plus) Lyophilisates 96 Tubes (8 strip × 12 ea)
43
BIOFACT CATALOGUE
LyoFACT™
OneStep RT-PCR Pre-Mix ⓗ
High Sensitive & High Specific RT-PCRs Convenient in Single Tubes
LyoFACT™ OneStep RT-PCR Pre-Mix is a lyophilized type designed for convenience and high e iciency in carrying out RT-PCR in a
single tube at one step. Included BioFACT™ RTase and BioFACT™ H-Star Taq DNA Polymerase is blended in one mixture with best
optimized rate to simplify reaction step and reducing the possibility of contamination. LyoFACT™ OneStep RT-PCR Pre-Mix has great
rst-strand cDNA yields by its thermostable characteristic at 50℃ for activation of BioFACT™ RTase and it allows robust, high speci c
ampli cation by BioFACT™ H-Star Taq DNA Polymerase included in the mixture. LyoFACT™ OneStep RT-PCR Pre-Mix o ers higher
sensitivity as it can increase the amount of template than solution-typed PCR Pre-Mix.
Feature
-
• Thermostable and RNase H variant of M-MLV RTase (RNase H ) • Especially the highest activity at 50℃
• Synthesize cDNA at 42℃ – 50℃ • Synthesis primer : gene specific primer
• Synthesis gene size : < 1 kb PCR • High specificity & Easy sample optimization
• cDNA synthesis & PCR in one sing tube • Low-copy transcripts amplification
• Great first-strand cDNA yields & Hot Start activity
Application
• RT-PCR
QC DATA
- Comparison of stability test of LyoFACT™ OneStep - Comparison of efficiency on various RNA concentrations
RT-PCR Pre-Mix over a long period of time between LyoFACT™ OneStep RT-PCR Pre-Mix and other
companies lyophilizate
0 month 2 month 6 month Company A BR531-096
M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 5 6 1 2 3 4 5 6
cDNA 4-Plex PCR Target : Human Total RNA
Target : Human Total RNA 1 : 50 ng 2 : 10 ng 3 : 2 ng 4 : 0.4 ng 5 : 0.08 ng 6 : 0.016 ng
1 : 100 ng 2 : 20 ng 3 : 4 ng 4 : 0. 8ng
44 BIOFACT CATALOGUE
DNA / RNA Amplification Lyophilized PCR Enzyme
LyoFACT™
OneStep RT-PCR Pre-Mix ⓗ - Comparison of amplification efficiency of LyoFACT™ OneStep RT-PCR Pre-Mix on various RNA concentrations
β-Actin (1 kb) BRCA2 (0.7 kb) β2M (1 kb) TNFR1 (0.6 kb) BRCA2 (0.4 kb)
M A B C D E F M M A B C D E F M M A B C D M M A B C D M M A B C D M
BIOFACT
Company A
Company B
Target : Human Total RNA PCR Condition : 60°C, 40 cycles Target : Human Total RNA PCR Condition : 60°C, 40 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 100 ng B : 20 ng C : 4 ng D : 0.8 ng E : 0.16 ng F : 0.032ng A : 100 ng B : 10 ng C : 1 ng D : 0.1 ng
Product Information
Pre-Mix Type
LyoFACT™ OneStep RT-PCR Pre-Mix ⓗ Lyophilisates, with dye [BR531-096]
▶Contents
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates, with dye
[96 Tubes]
▶Expiration date
1 Year at -20 ± 5℃
Maker Cat.No. Product Size
BIOFACT BR531-096 LyoFACT™ OneStep RT-PCR Pre-Mix ⓗ Lyophilisates, with dye 96 Tubes (8 strip × 12 ea)
45
BIOFACT CATALOGUE
LyoFACT™ OneStep
qRT-PCR Pre-Mix ⓗ (For Probe)
One-step RT-qPCR using RNA as a Template
LyoFACT™ OneStep RT-PCR Pre-Mix is a lyophilized type designed to perform one-step RT-qPCR in a single tube with convenience
and high e ciency. It is suitable for quantitative DNA analysis in real-time based on labeled probes (sequence speci c probe meth-
ods) and it is available with various Real-Time PCR cyclers. LyoFACT™ OneStep qRT-PCR Pre-Mix o ers higher sensitivity as it can
increase the amount of template than solution-typed PCR Pre-Mix.
Feature
• High sensitivity & Easy sample optimization • OneStep qRT-PCR
• Low copy transcripts amplification • Real-Time quantification of RNA target
• Use of any sequence-specific probe
Application
• Real-Time quanti cation of RNA target • Various application using qPCR
QC DATA
- Comparison of sensitivity (Limit of detection) in various concentrations of template DNA between LyoFACT™ OneStep
RT-PCR Pre-Mix (For probe) and BioFACT™ OneStep Multi-star qRT PCR Kit (For probe)
Ampli cation Plot
RQ321-10h
RQ501-096
Target : Human total RNA
Gene : β-2M [SFCgreen® I Dye]
Cycle
10 ng
△Ct Value 50 ng Ampli cation Plot 2 ng 0.4 ng
RQ321-10h 22.00 21.86 23.92 23.81 26.41 26.36 28.84 28.82
RQ501-096 21.95 22.02 23.81 23.93 26.51 26.69 28.96 29.06
Cycle
46 BIOFACT CATALOGUE
Ampli cation Plot
DNA / RNA Amplification Lyophilized PCR Enzyme
- Comparison of sensitivity (Limit of detection) in various TaqMan for LyoFACT™ OneStep RT-PCR Pre-Mix (For probe)
Cycle
Ampli cation Plot
Gene 1 [FAM]
Gene 2 [Cy5]
Multiplex PCR [Probe]
Target : Human Viral RNA
Gene : Gene 1 [FAM], Gene 2 [Cy5]
Cycle
△Ct Value 1.85 × 10 8 1.85 × 10 7 1.85 × 10 6 1.85 × 10 5 1.85 × 10 4
copies
copies
copies
copies
copies
Gene 1 [FAM] 5.57 9.14 12.36 15.64 19.16
Gene 2 [Cy5] 6.07 9.32 12.82 15.87 19.21
Product Information
Pre-Mix Type
LyoFACT™ OneStep qRT-PCR Pre-Mix ⓗ (For Probe) [RQ501-096]
▶Contents
LyoFACT™ OneStep qRT-PCR Pre-Mix (For Probe)
[96 Tubes]
▶Expiration date
1 Year at -20 ± 5℃
Maker Cat.No. Product Size
BIOFACT RQ501-096 LyoFACT™ OneStep qRT-PCR Pre-Mix ⓗ (For Probe) 96 Tubes (8 strip × 12 ea)
47
BIOFACT CATALOGUE
LyoFACT™ All in one Pre-Mix
Lyophilized Typed PCR Pre-Mix Including Primers
LyoFACT™ All in one PCR Pre-Mix includes primers on the kit and it can conveniently be used by adding template RNA & RNase free
water only. It reduces and minimizes the possibility of contamination & varation during PCR ampli cation.
Feature
• Source : Thermus aquaticus • 12-13 bp error / 10 bp
6
• 5' → 3' exonuclease activity : Yes • A-tailing : Yes
• 3' → 5' exonuclease activity (Fidelity) : No • Pre-Mix with primers on the package
• IT102A-096 [M13(-20)F,M13(-20)R] IT102B-096 [M13(-47)F,M13(-48)R] • High solubility
/ IT103-096 [E.coli MLST Primer Set] • Minimization of temperature restriction
Application
• Routine PCR • TA cloning
• PCR screening • Colony PCR
QC DATA
Plasmid & colony (IT102A-096, IT12B-096) PCR test
<Plasmid DNA, 500 bp> <Plasmid DNA, 1 kb>
Company A – Lyophilized Type [IT102A-096]-Lyophilized Type Company A – Lyophilized Type [IT102B-096]-Lyophilized Type
M A B C D E F NTC M A B C D E F NTC M M A B C D E F NTC M A B C D E F NTC M
[IT102A-096] LyoFACT™ All in one Colony PCR Pre-Mix (M13-20 primer) [IT102B-096] LyoFACT™ All in one Colony PCR Pre-Mix (M13-47 primer)
A : 10 0 (1 ng) B : 10 -1 C : 10 -2 D : 10 -3 E : 10 -4 F : 10 -5 A : 10 0 (1 ng) B : 10 -1 C : 10 -2 D : 10 -3 E : 10 -4 F : 10 -5
NTC : No-Template Control M. 1Kb DNA Ladder NTC : No-Template Control M. 1Kb DNA Ladder
<Plasmid DNA, 3kb> <Colony PCR>
Company A – Lyophilized Type [IT102A-096]-Lyophilized Type [IT102B-096] - Lyophilized Type
M A B C D E F NTC M A B C D E F NTC M M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 M NTC
[IT102A-096] LyoFACT™ All in one Colony PCR Pre-Mix (M13-20 primer) [IT102B-096] LyoFACT™ All in one Colony PCR Pre-Mix (M13-47 primer)
A : 10 0 (1 ng) B : 10 -1 C : 10 -2 D : 10 -3 E : 10 -4 F : 10 -5 NTC : No-Template Control
NTC : No-Template Control M. 1Kb DNA Ladder
48 BIOFACT CATALOGUE
DNA / RNA Amplification Lyophilized PCR Enzyme
E.coli MLST (IT103-096) PCR test
adk (583 bp) fumC (806 bp) gyrB (911 bp) icd (878 bp)
M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4
mdh (932 bp) fumC (806 bp) gyrB (911 bp)
M 1 2 3 4 M 1 2 3 4 M 1 2 3 4
Template : E.coli gDNA
1 : 10 0
2 : 10
-1
3 : 10
-2
4 : 10 -3
Product Information
Pre-Mix Type
LyoFACT™ MLST(E.coli) PCR Detection Kit, Lyophilisate, with dye [IT103-096]
▶Contents
MLST(E.coli) PCR Detection Kit
▶Expiration date
1 Year at -20 ± 5℃
Maker Cat.No. Product Size
IT102A-096 LyoFACT™ All in one Colony PCR Pre-Mix (M13F(-20), M13R(-20)) 96Tubes (8strip × 12)
BIOFACT IT102B-096 LyoFACT™ All in one Colony PCR Pre-Mix (M13F(-47), M13R(-48)) 96Tubes (8strip × 12)
IT103-096 LyoFACT™ All in one MLST PCR Pre-Mix (E.coli) 96 Tests *
* 96 Tests : 7ea Plates with 96 tubes (7ea primer sets)
49
BIOFACT CATALOGUE
DNA / RNA
Amplification
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
The words you are searching are inside this book. To get more targeted content, please make full-text search by clicking here.
Discover the best professional documents and content resources in AnyFlip Document Base.
Search