2022-23 BIOFACT Catalogue

Description 18

Lyophilized PCR Enzyme

LyoFACT™ PCR Pre-Mix Lyophilisates 40
LyoFACT™ RT Pre-Mix Lyophilisates 42
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates 44
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (For Probe) 46
LyoFACT™ All in one PCR Pre-Mix Lyophilisates 48

Real-Time PCR Enzyme
Intercalating Dye BioFACT™ Real-Time PCR Series 56
SFCgreen® I 57
BioFACT™ Real-Time PCR Series (With SFCgreen® I) 59
BioFACT™ Real-Time PCR Series (With SYBR® Green I) 64
BioFACT™ Real-Time PCR Series (With EvaGreen™) 65
20X EvaGreen™ 66
BioFACT™ OneStep qRT-PCR Master Mix 67
FastFACT™ Real-Time PCR Master Mix 69
Probe BioFACT™ Multi-Star Real-Time PCR Master Mix 70
BioFACT™ OneStep Multi-Star qRT-PCR Series 72
FastFACT™ qPCR Master Mix 74
FastFACT™ qRT-PCR Master Mix 75

PCR Enzyme

Universal PCR BioFACT™ F-Star Taq DNA Polymerase (Hot Start) 78
(<3 kb, 5 kb) BioFACT™ Thumb Taq DNA Polymerase 80
BioFACT™ Taq DNA Polymerase 82
Gene Speci c PCR BioFACT™ H-Star Taq DNA Polymerase (Hot Start) 84
(<1 kb, 5 kb) BioFACT™ A-Star Taq DNA Polymerase (Hot Start) 86

Long PCR BioFACT™ Lamp Taq DNA Polymerase 88
(<40 kb, λ DNA 기준) BioFACT™ A-Star Lamp Taq DNA Polymerase (Hot Start) 90
High Fidelity PCR BioFACT™ Pfu DNA Polymerase 92
(<3 kb, <20 kb) BioFACT™ Lamp Pfu DNA Polymerase 94
Multiplex PCR (<1 kb BioFACT™ 2X Multi-Star PCR Series (Hot Start) 96
PCR Inhibition SimpleWay™ 2X PCR Master Mix III 98


RT & RT-PCR Enzyme
BioFACT™ RT Series 102
BioFACT™ OneStep RT-PCR Series 104

Isothermal Amplification

BioFACT™ Bst DNA Polymerase 108

Other Reagent
BioFACT™ Uracil DNA Glycosylase (UDG) 112
BioFACT™ dNTP and dNTP Set 113
5X Band Helper™ 114
BioFACT™ RNase Inhibitor 116

Real-Time PCR Work flow












• Choose from singleplex, multiplex, low-throughput and high-throughput
Chemistry Selection qPCR depending on the type of analysis and decide according to purpose
(DNA binding Dyes whether to use DNA binding dyes, uorescent primer or probe type de-
or Probe type) pending on the cost of qPCR ampli cation, etc.
• In case of low-throughput singleplex qPCR, use DNA-binding dye such as
SYBR® Green I or EvaGreen™ Dye and in case of high-throughput qPCR, use
the uorescent primer or a probe.




• It is recommended to design the amplicon to be between 75 and 200bp.
• It is recommended to avoid the primer set that results in secondary structure or
Primer and binds to a long repeat single base (>4).
Probe Design • Ampli cation e ciency may be higher in case amplicon size is smaller.

• Annealing temperature should be set to 50 - 65℃ for qPCR ampli cation.
• It could be automatically designed when using the Primer 3
(Web-based primer design program, http://frodo.wi.mit.edu/applications/mfold/).




• In case speci city needs to be increased: Increase the annealing temperature (In-
hibits non-speci c ampli cation or primer dimer formation).
qPCR Optimization • Test the reproducibility of the ampli cation e ciency and the uorescence dy-
namic range of the primer designed through serial dilution of the template with
known concentration.
• Analysis e ciency should be 90 - 105% and R2 value from the standard curve
should be >0.980 or higher.
design program, http://frodo.wi.mit.edu/applications/mfold/).




• In case intercalating dye was used: Through checking not only the ampli cation
curve but also the melting curve, check whether there are any non-speci cally
Data Analysis ampli ed bands except the target gene.
(For Graph)
• Compare the Ct values between samples after checking that R2 of the standard
curve is close to 1 (maximum 0.99).








52 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme





Fluorescence
Real-Time PCR Work flow
Detection System











• BioFACT™ RT Series (p.102)
DNA RNA







OneStep
qRT-PCR



Intercalating dye Probe
• BioFACT™ Real-Time PCR Series
(For Intercalating Dye) (p.56)








SFCgreen® I Dye SYBR® Green I Dye EvaGreen™ Dye TaqMan® Probe

• 2X Real-Time PCR Master Mix • 2X Real-Time PCR Master Mix • Real-Time PCR • 2X Multi-Star Real-Time PCR Master Mix
Standard Type
• 2X Real-Time PCR Pre-Mix • 2X Real-Time PCR Pre-Mix • OneStep Multi-Star qRT-PCR Series
• 2X Real-Time PCR
Master Mix

SFCgreen® I Dye SYBR® Green I Dye EvaGreen™ Dye TaqMan® Probe

Sensitivity **** ** *** ****
Specificity * * * ****
Fluorescent signal ***** ** **** **
Multiplex PCR Impossible Impossible Impossible Possible
Melting curve required required required Not required
Primer & Probe design Easy Easy Easy Di cult








53
BIOFACT CATALOGUE

Fluorescence

Detection System

F
F
F F
F : uorescence
F F

F
F
DNA-Binding Dye System
F F
F : uorescence
This method detects the uorescence of dye bound to the ampli ed DNA (or cDNA). SFCgreen® I, SYBR® Green I and EvaGreen™
F
F
dye are typically used for this system. A false signal may occur due to non-speci c ampli cation, so the melting curve should
SYBR® Green I
be analyzed together. F F F F unbound-no
uorescence Forward TaqMan Q
primer probe
R Primer and
Probe-base Detection System (annealing and extension) probe annealing
PCR ampli cation
F
F
In addition to the ampli cation primer, a probe to which a uorescent dye is bound is also added for detection. Target-speci c
F
F
F : uorescence
Fluorescence
Fluorescence
probes do not require melting curve analysis, and multiplex PCR is available as the region combined with Q-R primer can be ampli-
F
SYBR® Green I
F
F
F
unbound-no
F
F
F
ed at the same time. In this case, di erent types of reporter (dye) should be used for detection. (e.g. FAM, HEX, VIC, ROX, Cy5 etc.) Forward TaqMan
F
uorescence
F
F
primer probe Q
F F F F R Primer and
PCR ampli cation probe annealing
Principle of the Intercalating Dye F F F F (annealing and extension)
F : Fluorescence Fluorescence
< SYBR® Green I Dye > < EvaGreen™ Dye > Fluorescence Fluorescence
EvaGreen™ has the same principle of action as SYBR® Green I, but exhibits R
F
F
SYBR® Green I F F R
F F F F unbound-no much higher sensitivity and DNA binding capacity than SYBR® Green I due Q
F
F
F
F
uorescence Forward TaqMan Q Extension cleavage of
to the "release on demand" feature. In addition, EvaGreen™ is character- uorescent label
primer
probe
F
F
R
F
F
Primer and
probe annealing
PCR ampli cation ized by being non-toxic and non-mutagenic, making it safe for users. Fluorescence
: Fluorescence
F
(annealing and extension) R R
Novel “Release-on-Demand” DNA Binding Mechanism
R
Fluorescence Fluorescence R
DNA Q
F F
Extension cleavage of
F F : Taq polymerase
F F F F EvaGreen™ , EvaGreen™ , EvaGreen™ - DNA complex uorescent label
inactive form active form R : Reporter (형광물질)
F F F F R R
Fluorescence
B) 30 min.
F : Fluorescence A) 5 min. Novel “Release-on-Demand” DNA Binding Mechanism Q : Quencher (상쇄물질)
B) 30 min.
incubation, long photographing
incubation incubation exposure time
DNA
R
<SFCgreen® Ⅰ> R : Taq polymerase
SFCgreen® I has the same principle of action as SYBR® green I or SYBR® EvaGreen™ , EvaGreen™ , Q EvaGreen™ - DNA complex R : Reporter (형광물질)
inactive form
active form
Green I Extension cleavage of
EvaGreen™, but has higher DNA binding strength and higher uorescent label
B) 30 min. Q : Quencher (상쇄물질)
sensitivity. As it has lower PCR inhibition than other intercalating A) 5 min. R B) 30 min. incubation, long photographing
incubation R
exposure time
incubation
Novel “Release-on-Demand” DNA Binding Mechanism
dyes, PCR ampli cation e ciency is high even at high concentra-
tions, and it is stable under light, heat, and chemicals, resulting
DNA
in a longer uorescence half-life compared to other uorescent SYBR®
Green I
EvaGreen™ : Taq polymerase
dyes.
EvaGreen™ , EvaGreen™ , EvaGreen™ - DNA complex
inactive form active form Minor groove binder Bis-intercalator R : Reporter (형광물질)
B) 30 min. Q : Quencher (상쇄물질)
A) 5 min. B) 30 min. incubation, long photographing
incubation incubation exposure time
Major groove Minor groove
EvaGreen™
SYBR®
Green I Principle of Probe Reporter-Quencher (Hydrolysis) Fluorescence
R
R
Forward TaqMan Q
primer probe Q
R
: Taq polymerase
EvaGreen™ R
R
R : Reporter (형광물질)
Reporter (Fluorescent substance)
Primer and Extension cleavage of Q Quencher (O setting substance)
: Quencher (상쇄물질)
probe annealing uorescent label
54 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme





What is Passive Reference



Dye? (e.g. ROX,Fluorescein dye etc.)







Passive reference dye is used as a reference point to correct the uorescence signal in real-time PCR or to calculate the uctuation
of the uorescence signal. Calibration means modifying to minimize the di erence between the total volume or the equipment
scanner during the reaction. In general, most real-time PCR equipment uses ROX dye as a passive reference dye. This is because
ROX dye does not have any e ect on real-time PCR reaction and emits a uorescence signal that is clearly distinguished from probe
uorescent dye (reporter or quencher). As with some equipment from Bio-Rad, there are some that use uorescein dye and there
are systems that do not require a passive dye such as the Rotor-gene. It is recommended to select the passive reference dye to be
used after considering the advantages and disadvantages of each device or method.

Required , Optional

Agilent (Stratagene) Mx3000P, Mx3005P, Mx4000, AriaMx
Analytik Jena qTOWER
Bioer LineGene, LineGene K, LineGene 9600, LineGene 9640, LineGene 9660
Bioneer Exicycler96
Bio-Rad CFX96, CFX384, Chromo4, MiniOpticon, Opticon, Opticon2

BJS Xxpress
Cepheid SmartCycler, SmartCycler2
Eppendorf Mastercycler ep realplex, Mastercycler ep realplex-2S
Low-ROX Fluidigm BioMark
Hain Lifescience FluoroCycler 96
Illumina Eco
Life Technologies 7500, 7500 FAST, QuantStudio 12k Flex, ViiA7

Qiagen Rotor-Gene 3000, Rotor-Gene 6000, Rotor-Gene Q
Roche LightCycler 1, LightCycler 2, LightCycler 96, LightCycler 480, LightCycler nano
Takara Thermal Cycler Dice (TP800)
Techne PrimeQ, Quantica
Thermo Piko Real


Life Technologies PRISM 5700, 7000, 7300, 7700, 7900, 7900HIT, 7900HIT FAST
High-ROX
StepOne, StepOnePlus


Fluorescein Bio-Rad iCycler iQ, MyiQ, MyiQ2, MyiQ5





55
BIOFACT CATALOGUE

BioFACT™ Real-Time


PCR Series ⓗ (For Intercalating Dye)


Real-Time PCR Series






BioFACT™ Real-Time PCR Series is providing optimizing system for easy-use using various intercalating reagents in Real-Time PCR am-
pli cation and users can use their own intercalating dyes by themselves. Furthermore, EvaGreen™ or SYBR® Green I can be supplied
with our kits as an option or mixture types for convenient use and included BioFACT™ H-Star Taq DNA Polymerase helps accurate and
reliable result to enable target-speci c ampli cation due to its Hot Start characteristic.
BioFACT™ 2X Real-Time PCR Master Mix and Pre-Mix contains H-Star Taq DNA polymerase, dNTPs, optimized reaction bu er, passive
reference dye (in case of Pre-Mix type) and intercalating dyes (EvaGreen™/SYBR® Green I). Your template and primers are the only
additions necessary to perform qPCR reaction.



Application
• Quantification of target DNA using Real-Time PCR • Various applications using qPCR
• Quantification of target RNA using RT-PCR • High resolution melting (HRM) analysis






Product Information

Standard Type (w/o Intercalating Dye)
BioFACT™ Real-Time PCR Kit ⓗ [DQ116-250]

▶Contents
BioFACT™ H-Star Taq DNA Polymerase (2.5 U/㎕)
10X Reaction Bu er (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix
(dATP, dTTP, dGTP, dCTP)

▶ Expiration date
2 Year and 3 months at -20±5℃










Type Cat.No. Product Size
DQ116-250 BioFACT™ Real-Time PCR Kit with 10 mM dNTP Mix ⓗ (each 10 mM, 0.2 ㎖), 25mM MgCl2, w/o Intercalating Dye 250 U
Standard
DQ116-500 BioFACT™ Real-Time PCR Kit with 10 mM dNTP Mix ⓗ (each 10 mM, 0.4 ㎖), 25mM MgCl2, w/o Intercalating Dye 500 U





56 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme





SFCgreen® I




Outstanding Intercalating Dye 100% Made in Korea






SFCgreen® I is a green uorescent dye with excitation and emission wavelengths very similar to those of SYBR® green I or Eva-
Green™. The DNA binding mechanism is a bis-intercalator that binds to the DNA minor groove and emits light.
SFCgreen® I has high DNA binding strength and shows a higher sensitivity compared to SYBR® green I or EvaGreen™. Because
PCR inhibition is low, PCR ampli cation e ciency is high even at high concentrations, and it is stable under light, heat, and
chemicals, resulting in a longer uorescence half-life compared to other uorescent dyes.

Feature
• λ abs / λ em = 459/522 nm (DNA bound) • Superior bright fluorescence & Long half-life
• Similar wavelength with that for SYBR®green I or EvaGreen™ • HRM / Melting curve analysis available
• Less PCR inhibition by dye concentration • Non-mutagenic, Non-cytotoxic




SFCgreen® I Wavelengths for SFCgreen® I, SYBR®Green I & EvaGreen™
100
90 excitation emission
fluorescence excitation or emission(%) 60 50 40 30
Minor groove binder Bis-intercalator 80 70


10 20
Major groove Minor groove 0
300 350 400 450 500 550 600 650 700
Wavelength (nm)
Dye Excitation Emission
SFCgreen® I 459 nm 522 nm
EvaGreen™ 500 nm 530 nm
SYBR®Green I 497 nm 520 nm

QC DATA
Comparison of thermal stability between SFCgreen® I and EvaGreen™
Melt Curve
[Idle time test]
0.059 Melt Curve
0.100 0.059
0.100 EvaGreen TM SFCgreen® I
EvaGreen TM SFCgreen® I
0.049
0.080 SFCgreen® I 0.049
0.080 SFCgreen® I 0.039
0.060 0.039
0.060 Derivative Reporter (-Rn) Derivative Reporter (-Rn)
0.040 0.029 0.029
0.040
0.019
0.019
0.020
0.020
0.0090 0.0090
0.000
0.000
0 hr
0 hr 0.5 hr 1 hr 1 hr 2 hr 2 hr 3 hr 3 hr 65.0 70.0 75.0 80.0 85.0 90.0 95.0
0.5 hr
65.0 70.0 75.0 80.0 85.0 90.0 95.0
Temperature (℃) Temperature (℃)
: 95℃ / 0 hr : 95℃ / 0.5 hr : 95℃ / 1 hr : 95℃ / 2 hr : 95℃ / 3 hr
* SFCgreen® I dye has better thermostability than EvaGreen™.
(Small deviation in absorbance compared to EvaGreen™)
57
BIOFACT CATALOGUE

- Excellent stability under light - Check on safety for chemicals


* Photobleaching Test * Verified through Ames Test
<Comparison of Stability under light between SFCgreen® I and SYBR® Green I> <Non-mutagenic & Non-toxic>
<SYBR® Green I> <SFCgreen® I>
260000 5000000
Concentration 0.5x Concentration 1x
250000 Half-life 5.5 hr 4000000 Half-life 9 hr
240000
230000 3000000
220000 2000000
210000
1000000
200000
190000 0
1 min 30 min 60 min 1 min 30 min 60 min
230000 4500000
Concentration 0.25x Concentration 0.5x
220000 Half-life 5.5 hr 3500000 Half-life 9 hr
210000
2500000
200000
1500000
190000
180000 500000
170000 (500000)
1 min 30 min 60 min 1 min 30 min 60 min
220000 300000
Concentration 0.125x Concentration 0.25x
210000 Half-life 5.5 hr Half-life 10.5 hr
200000 250000
190000
200000
180000
170000
150000
160000
150000 100000
1 min 30 min 60 min 1 min 30 min 60 min


Product Information

20X SFCgreen® I [FD306-50h] 2,000X SFCgreen® I [FD301-250]








▶Contents ▶ Expiration date ▶Contents ▶ Expiration date
20X SFCgreen® I 1 Year at -20 ± 5℃ 2.000X SFCgreen® I 1 Year at -20 ± 5℃
[1 ㎖ × 5 ea] [50 ㎕ × 5]





Maker Cat.No. Product Size
FD306-50h 20X SFCgreen® I Dye 1 ㎖ x 5ea
BIOFACT
FD301-250 2,000X SFCgreen® I Dye 50 ㎕ × 5ea





58 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme



BioFACT™ Real-Time


PCR Series ⓗ (With SFCgreen® I)


Accurate and Highly Sensitive Real-Time PCR using SFCgreen® I






BioFACT™ 2X Real-time PCR Kit using SFCgreen® I contains H-Star Taq DNA polymerase with Hot Start PCR function, so that only target
DNA is speci cally ampli ed. BioFACT™ 2X Real-time PCR Kit is a pre-mixed form of H-Star Taq DNA Polymerase, dNTP, Reaction bu er
and SFCgreen® I, so that it can be conveniently used by simply adding the primer and the template to perform qPCR rection.
The use of passive reference dye di ers depending on the model, so we have produced a variety of products suitable for many models
to increase user convenience. Also, for pre-mixed products, it is available in three types: 0.1㎖ 8-strip Frosted Tube, White Tube, and Clear
Tube, so it can be used in various models.


Feature
• Highly specific amplification using • Excellent sensitivity & fluorescence against heat,
BioFACT™ H-Star Taq DNA polymerase light and chemicals
• Pre-denaturation time : 15 mins. • Suitable for UDG system



Application
• Quanti cation of target DNA using Real-Time PCR • Application using qPCR
• Quanti cation of target RNA using RT-PCR • High Resolution Melting (HRM) analysis





QC DATA

- Comparison of PCR inhibition test using fluorescent dyes between EvaGreen™ & SFCgreen® I


< EvaGreen™ > < SFCgreen® I >

Ampli cation P Ampli cation Plot Ampli cation P Ampli cation Plot
lot
lot
25.0 25.0 25.0 25.0
1x
22.5 22.5 22.5 22.5
20.0 20.0 20.0 20.0
0.75x
17.5 17.5 17.5 17.5
15.0 15.0 15.0 15.0
△Rn 12.5 △Rn 12.5 △Rn 12.5 △Rn 12.5 0.5x
10.0 10.0 1x 10.0 10.0
7.5 7.5 0.75x 7.5 7.5 0.25x
5.0 5.0 0.5x 5.0 5.0
2.5 2.5 0.25x 2.5 2.5
0.0 0.0 0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle Cycle Cycle Cycle



59
BIOFACT CATALOGUE

- Comparison of sensitivity of 3 types of fluorescent dyes (SFCgreen® I vs SYBR® Green I vs EvaGreen™)


DCt value
Ampli cation Plot HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
16
15 SFCgreen® I 21.39 21.36 23.61 23.56 25.93 25.81 28.21 27.95
14 SFCgreen® I
13 EvaGreen™(DQ381) 23.12 23.21 25.4 25.47 27.55 27.88 29.96 30.14
12
11 EvaGreen TM SYBR® Green I(DQ383) 23.23 23.36 25.65 25.62 27.86 27.98 30.21 30.10
10 (DQ381)
9
△Rn 8
7 ΔRn value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
6
5 [HSP89 Gene] SFCgreen® I 14.75 14.35 14.58 14.28 14.35 14.4 14.33 14.53
4
3 SYBR® Green I EvaGreen™(DQ381) 11.86 11.85 11.98 11.92 11.64 11.76 10.96 10.69
2 (DQ383)
1 SYBR® Green I(DQ383) 3.19 3.14 3.31 3.34 3.36 3.38 3.22 3.27
0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle * Ampli cation e ciency : SFCgreen® I > EvaGreen™(DQ381) > SYBR® Green I(DQ383)
Ampli cation Plot
17
16 SFCgreen® I
15
14
13 EvaGreen TM DCt value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
12 Ampli cation Plot (DQ381)
SFCgreen® I 15.94 15.86 18.18 18.44 20.47 20.5 23.14 23.13
11 16
10 15
△Rn 9 14 SFCgreen® I
8 EvaGreen™ (DQ381) 18.26 18.38 20.59 20.58 22.73 22.8 25.75 25.71
7 13
6 12 SYBR® Green I(DQ383) 18.62 18.6 20.7 20.73 22.97 22.9 25.68 25.71
5 11 [HSP89 Gene]
4 10 SYBR® Green I
3 9
2 △Rn 8 (DQ383) ΔRn value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
1 7 EvaGreen TM
(DQ381)
0 6 SFCgreen® I 14.64 14.5 14.65 13.03 14.66 15.01 14.52 14.71
-1 5 [GAPDH Gene]
4 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 EvaGreen™ (DQ381) 6.82 6.94 7.23 7.08 7.15 7.09 6.83 6.74
Cycle
3 SYBR® Green I
2 (DQ383)
1 SYBR® Green I(DQ383) 1.8 1.81 1.92 1.88 1.94 1.97 1.96 1.95
0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 * Ampli cation e ciency : SFCgreen® I > EvaGreen™(DQ381) > SYBR® Green I(DQ383)
Cycle
Ampli cation Plot
16
15
14 SFCgreen® I
13
12
11 Ampli cation Plot EvaGreen TM
10 17 (DQ381)
9 16 SFCgreen® I
△Rn 8 15
7 14
6 13 EvaGreen TM
5 12 [HSP89 Gene] (DQ381)
4 11
3 10 SYBR® Green I
9
△Rn
- Comparison of sensitivity of 3 types of fluorescent dyes (SFCgreen® I vs SYBR® Green I vs EvaGreen™), UDG system
(DQ383)
2 8
1 7
0 6
5 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
[MAPK1 Gene]
4 Cycle SYBR® Green I
3 (DQ383)
2
1
0
-1
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Ampli cation Plot ΔCt value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
Cycle
17
16 SFCgreen® I
15 SFCgreen® I 21.42 21.47 23.78 23.79 26.31 25.94 28.56 28.68
14
13 EvaGreen TM EvaGreen™(DQ381) 23.13 23.18 25.42 25.45 27.62 27.84 30.07 30.15
12 (DQ381)
11 Ampli cation Plot SYBR® Green I(DQ383) 23.33 23.28 25.71 25.57 27.76 27.8 30.12 29.93
10 16
△Rn 9 15 814 SFCgreen® I
713 ΔRn value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
612
[HSP89 Gene] SFCgreen® I 15.96 15.91 16.07 16.21 12.82 15.84 15.43 14.5
5 11
4 10 SYBR® Green I
3 9 (DQ383)
△Rn 2 8 EvaGreen™(DQ381) 11.05 11.62 10.74 11.77 11.53 11.34 10.72 10.54
1 7 EvaGreen TM
0 6 (DQ381) SYBR® Green I(DQ383) 3.54 3.53 3.65 3.71 3.69 3.70 3.57 3.60
-1 5
[GAPDH Gene]
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
4 Cycle
3 * Ampli cation e ciency : SFCgreen® I > EvaGreen™(DQ381) > SYBR® Green I(DQ383)
2 SYBR® Green I
1 (DQ383)
0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle
Ampli cation Plot ΔCt value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
17
16 SFCgreen® I SFCgreen® I 20.92 21.02 23.33 23.33 25.39 25.59 27.88 27.94
15
14
13 EvaGreen TM EvaGreen™ (DQ381) 22.72 22.62 25.25 25.21 27.46 27.2 29.7 29.28
12 (DQ381)
11 SYBR® Green I(DQ383) 22.85 22.87 25.3 25.2 27.56 27.62 30.18 29.63
10
△Rn 9 8
7 ΔRn value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
6
5 [MAPK1 Gene] SFCgreen ® I 16.37 16.12 16.29 16.35 16.14 16.02 15.68 15.57
4 SYBR® Green I
3 (DQ383)
2 EvaGreen™ (DQ381) 12.74 12.78 12.71 12.87 11.4 11.18 10.74 9.69
1
0 SYBR® Green I(DQ383) 3.52 3.57 3.58 3.67 3.56 3.62 3.36 3.40
-1
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle
* Ampli cation e ciency : SFCgreen® I > EvaGreen™(DQ381) > SYBR® Green I(DQ383)
60 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme







Product Information

Master Mix Type (Including SFCgreen® I ) Pre-Mix Type (Including SFCgreen® I)
BioFACT™ 2X Real-Time PCR Master Mix ⓗ [DQ362-40h] BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ [DQ462-096]












▶Contents ▶ Expiration date ▶Contents ▶ Expiration date
2X Real-Time PCR Master Mix 1 Year at -20 ± 5℃ 2X Real-Time PCR Pre-Mix 1 Year at -20 ± 5℃
(including SFCgreen® I ) (including SFCgreen® I)
[1 ㎖ x 4 ea] [96Tubes (8strips × 12)]






























Type Cat.No. Product Size
DQ362-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ , including SFCgreen® I in mixture, Low Rox reference dye 1 ㎖ × 4 ea
DQ363-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ , including SFCgreen® I in mixture, High Rox reference dye 1 ㎖ × 4 ea
Master
Mix DQ367-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ , including SFCgreen® I in mixture, Low Rox reference dye (UDG System) 1 ㎖ × 4 ea
DQ368-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ , including SFCgreen® I in mixture, High Rox reference dye (UDG System) 1 ㎖ × 4 ea
DQ462-096
(W/F/C) BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ , including SFCgreen® I in mixture, Low Rox reference dye 96Tubes (8strip × 12)
DQ463-096(F) BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ , including SFCgreen® I in mixture, High Rox reference dye 96Tubes (8strip × 12)
Pre-Mix
DQ467-096
(W/F/C) BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ , including SFCgreen® I in mixture, Low Rox reference dye (UDG System) 96Tubes (8strip × 12)
DQ468-096(F) BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ , including SFCgreen® I in mixture, High Rox reference dye (UDG System) 96Tubes (8strip × 12)
* The products containing low ROX allow choosing from White Tube(W), Frosted Tube(F), or Clear Tube(C).
* The product containing high ROX is sold with Frosted Tube (F) type only.




61
BIOFACT CATALOGUE

BioFACT™ Real-Time


PCR Series ⓐ (With SFCgreen® I)


Accurate and Rapid Real-Time PCR Series using SFCgreen® I






BioFACT™ 2X Real-time PCR Kit using SFCgreen® I contains A-Star Taq DNA polymerase with hot start PCR function, so that only
target DNA is speci cally ampli ed. BioFACT™ A-Star 2X Real-time PCR Kit is a pre-mixed type of A-Star Taq DNA Polymerase, dNTP,
Reaction bu er and SFCgreen® I, so that it can be conveniently used by simply adding the primer and the template to perform
qPCR reaction. The use of passive reference dye di ers depending on the model, so we have produced a variety of products suita-
ble for many models to increase user convenience. Also, for pre-mixed products, it is available in three types: 0.1㎖ 8-strip Frosted
Tube, White Tube, and Clear Tube, so it can be used in various models.


Feature
• Highly specific amplification using • Excellent sensitivity & fluorescence against heat,
BioFACT™ A-Star Taq DNA polymerase light and chemicals
• Pre-denaturation time : 2 mins. • Suitable for UDG system


Application
• Quanti cation of target DNA using Real-Time PCR • Application using qPCR
• Quanti cation of target RNA using RT-PCR • High resolution melting (HRM) analysis





QC DATA
- Comparison of PCR inhibition test using fluorescent dyes between EvaGreen™ & SFCgreen® I
A-Star Taq/dNTP(T) System
< EvaGreen™ > < SFCgreen® I >
Ampli cation Plot Ampli cation Plot
30.0 30.0
27.5 27.5 1X
25.0 25.0
22.5 22.5
1X
20.0 20.0 0.75X
0.75X
17.5 17.5
△Rn 15.0 △Rn 15.0
12.5 12.5 0.5X
10.0 0.5X 10.0
7.5 [MAPK1 Gene] 7.5 0.25X
5.0 0.25X 5.0
2.5 2.5
0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22
Cycle Cycle
Ampli cation Plot Ampli cation Plot
30.0 30.0
27.5 27.5 1X
25.0 25.0
22.5 22.5
0.75X
20.0 20.0
17.5 17.5
△Rn 15.0 △Rn 15.0 0.5X
12.5 12.5
10.0 1X 10.0
7.5 [GAPDH Gene] 0.75X 7.5 0.25X
0.5X
5.0 5.0
2.5 0.25X 2.5
0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22
Cycle Cycle
62 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme





- Comparison of PCR inhibition test using fluorescent dyes between EvaGreen™ & SFCgreen® I, UDG system
A-Star Taq/dNTP(U) System
< EvaGreen™ > < SFCgreen® I >
Ampli cation Plot Ampli cation Plot
30.0 30.0 1X
27.5 27.5
25.0 1X 25.0
22.5 22.5
0.75X
20.0 20.0
17.5 0.75X 17.5
△Rn 15.0 △Rn 15.0 0.5X
12.5 0.5X 12.5
10.0 10.0
7.5 [MAPK1 Gene] 0.25X 7.5 0.25X
5.0 5.0
2.5 2.5
0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22
Cycle Cycle
Ampli cation Plot Ampli cation Plot
30.0 30.0
27.5 27.5 1X
25.0 25.0
22.5 22.5
0.75X
20.0 20.0
17.5 17.5
△Rn 15.0 △Rn 15.0 0.5X
12.5 12.5
10.0 1X 10.0
7.5 [GAPDH Gene] 7.5 0.25X
0.75X
5.0 0.5X 5.0
2.5 0.25X 2.5
0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22
Cycle Cycle
Product Information
Master Mix Type (Including SFCgreen® I ) Pre-Mix Type (Including SFCgreen® I)
BioFACT™ 2X Real-Time PCR Master Mix ⓐ [DQ352-40h] BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ [DQ452-096(W)]







▶Contents ▶ Expiration date ▶Contents ▶ Expiration date
2X Real-Time PCR Master Mix 1 Year at -20 ± 5℃ 2X Real-Time PCR Pre-Mix 1 Year at -20 ± 5℃
(including SFCgreen® I) (including SFCgreen® I)
[1 ㎖ x 4 ea] [96Tubes (8strip × 12)]





Type Cat.No. Product Size
DQ352-40h BioFACT™ 2X Real-Time PCR Master Mix ⓐ, including SFCgreen® I in mixture, Low Rox reference dye 1 ㎖ × 4 ea
Master DQ353-40h BioFACT™ 2X Real-Time PCR Master Mix ⓐ, including SFCgreen® I in mixture, High Rox reference dye 1 ㎖ × 4 ea
Mix DQ357-40h BioFACT™ 2X Real-time PCR Master Mix ⓐ, including SFCgreen® I in mixture, Low Rox reference dye (UDG System) 1 ㎖ × 4 ea
DQ358-40h BioFACT™ 2X Real-Time PCR Master Mix ⓐ, including SFCgreen® I in mixture, High Rox reference dye (UDG System) 1 ㎖ × 4 ea
DQ452-096(W/F/C) BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ, including SFCgreen® I in mixture, Low Rox reference dye 96Tubes (8strip × 12)
DQ453-096(F) BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ, including SFCgreen® I in mixture, High Rox reference dye 96Tubes (8strip × 12)
Pre-Mix
DQ457-096(W/F/C) BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ, including SFCgreen® I in mixture, Low Rox reference dye (UDG System) 96Tubes (8strip × 12)
DQ458-096(F) BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ, including SFCgreen® I in mixture, High Rox reference dye (UDG System) 96Tubes (8strip × 12)
* The products containing low ROX allow choosing from White Tube(W), Frosted Tube(F), or Clear Tube(C).
* The product containing high ROX is sold with Frosted Tube (F) type only.



63
BIOFACT CATALOGUE

BioFACT™ Real-Time


PCR Series ⓗ (For SYBR® Green I)


Accurate and Sensitive Real-Time PCR Assays using SYBR® Green I






- Comparison of activity in various concentrations of template between BioFACT™ 2X Real-Time PCR Master Mix
(For SYBR® Green I) and other Real-Time PCR Master mixes from other companies


△Ct Value 50 ng 10 ng 2 ng 0.4 ng
BIOFACT 18.27 21.18 24.97 28.78
BIOFACT Company A
Company A 19.80 23.24 27.64 32.35
Company B 29.31 26.77 30.79 35.18
Company C 18.35 21.76 25.68 29.35
Target : Human genomic DNA
Company B Company C Gene : MAPK1




Product Information
Master-Mix Type (Including SYBR® Green I) Pre-Mix Type (Including SYBR® Green I)
BioFACT™ 2X Real-Time PCR Master Mix ⓗ [DQ383-40h] BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ [DQ483-096]











▶Contents ▶ Expiration date ▶Contents ▶ Expiration date
2X Real-Time PCR Master Mix 6 months at -20 ± 5℃ 2X Real-Time PCR Pre-Mix 1 Year at -20 ± 5℃
(including SYBR® Green I) (SYBR® Green I kit)
[1 ㎖ x 4 ea] [96 tubes]



Type Cat.No. Product Size
DQ383-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), including SYBR® Green I 1 ㎖ x 4 ea
in mixture, w/o Rox reference dye
Master Mix DQ384-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), including SYBR® Green I 1 ㎖ x 4 ea
in mixture, with low Rox reference dye
BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), including SYBR® Green I
DQ385-40h 1 ㎖ x 4 ea
in mixture, with high Rox reference dye
DQ483-096 BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ including SYBR® Green I in mixture, w/o Rox reference dye 96 Tubes (8 strip x 12 ea)
Pre-Mix DQ484-096(W/F/C) BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ including SYBR® Green I in mixture, with low Rox reference dye 96 Tubes (8 strip x 12 ea)
DQ485-096(F) BioFACT™ 2X Real-Time PCR Pre-Mix ⓗ including SYBR® Green I in mixture, with high Rox reference dye 96 Tubes (8 strip x 12 ea)
* The products containing low ROX allow choosing from White Tube(W), Frosted Tube(F), or Clear Tube(C).
* The product containing high ROX is sold with Frosted Tube (F) type only.


64 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme



BioFACT™ Real-Time


PCR Series ⓗ (With EvaGreen™)


Highly Sensitive and Accurate Real-Time PCR using EvaGreen™






- Comparison of sensitivity in various concentrations of template DNA between BioFACT™ 2X Real-Time PCR Kit
(with EvaGreen™) and other company’s Real-Time PCR kit (Limit of Detection)

△Ct Value 50 ng 10 ng 2 ng 0.4 ng
BIOFACT 18.26 21.52 25.60 29.27
Company A 21.63 24.94 28.93 32.73
BIOFACT Company A
Target : Human genomic DNA
Gene : MAPK1

Product Information
Standard Type (With EvaGreen™)
BioFACT™ Real-Time PCR Kit ⓗ [DQ191-250]
▶Contents ▶ Expiration date
EvaGreen™ (20X in water) 1 Year at -20 ± 5℃
BioFACT™ H-Star Taq DNA Polymerase (2.5 U/㎕)
10X Reaction Bu er (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)


Master Mix Type (With EvaGreen™) Master Mix Type (Including EvaGreen™)
BioFACT™ 2X Real-Time PCR Master Mix ⓗ [DQ371-40h] BioFACT™ 2X Real-Time PCR Master Mix ⓗ [DQ381-40h]









▶Contents ▶ Expiration date ▶Contents ▶ Expiration date
EvaGreen™ (20X in water) 1 Year at -20 ± 5℃ 2X Real-Time PCR Master Mix 1 Year at -20 ± 5℃
2X Real-Time PCR Master Mix (including EvaGreen™)
[1 ㎖ x 4 ea] [1 ㎖ x 4 ea]
Reference
1. Triterpene and Flavonoid Biosynthesis and Metabolic Pro ling of Hairy Roots, Adventitious Roots, and Seedling Roots of Astragalus membranaceus. Park YJ1,ThweAA1,
Li X1, Kim YJ1, Kim JK2, Arasu MV, Al-Dhabi NA, Park SU1./J Agric Food Chem. 2015 Oct 14;63(40):8862-9. doi: 10.1021/acs.jafc.5b02525. Epub 2015 Oct 1
Type Cat.No. Product Size
DQ191-250 BioFACT™ Real-Time PCR Kit with 10 mM dNTP Mix ⓗ (each 10 mM, 0.2 ㎖), 25mM MgCl2, with EvaGreen™ 500 ㎕ 250 U
Standard
DQ191-500 BioFACT™ Real-Time PCR Kit with 10 mM dNTP Mix ⓗ (each 10 mM, 0.4 ㎖), 25mM MgCl2, with EvaGreen™ 1㎖ 500 U
DQ371-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), with EvaGreen™ 500 ㎕ 1 ㎖ x 4 ea
DQ372-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), w/o EvaGreen™ 500 ㎕ 1 ㎖ x 4 ea
Master Mix
DQ381-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), including EvaGreen™ in mixture 1 ㎖ x 4 ea
DQ376-40h BioFACT™ 2X Real-Time PCR Master Mix ⓐ, including EvaGreen™ in mixture, with low Rox reference dye 1 ㎖ x 4 ea


65
BIOFACT CATALOGUE

20X EvaGreen™



Intercalating Dye with High Sensitivity and Stability







20X EvaGreen™ is the next generating DNA binding dye that can be used in Real-Time PCR. EvaGreen™ is compatible with HRM
analysis as it has less PCR interference, such as slowing down the primer dimer formation and extension reaction, as well as high
sensitivity to double-stranded DNA, and can produce more accurate results. It has little PCR inhibition even at high concentrations
and is compatible with most qPCR instruments in a wavelength similar to SYBR® Green I. It is non-mutagenic, noncytotoxic, and very
stable under various PCR conditions.
Application
• λabs/λem = 500/530 nm (DNA bound) • Less PCR inhibition by dye concentration
• λabs = 471 nm (w/o DNA) • Superior bright fluorescence & Long half-life
• Similar wavelength with that for SYBR®green I or FAM • HRM / Melting curve analysis available
• Non-mutagenic, Non-cytotoxic



Comparison test between EvaGreen™ and SYBR® Green I



△Ct Value 50 ng 10 ng 2 ng 0.4 ng
EvaGreen™ 18.26 21.52 25.60 29.27
SYBR® Green I 18.27 21.18 24.97 28.78
Target : Human genomic DNA
Gene : MAPK1
EvaGreen™ SYBR® GreenⅠ


Product Information
20X EvaGreen™ [31000-B500]

▶Contents
20X EvaGreen™ [500 ㎕]
▶Expiration date
1 Year at -20 ± 5℃



Reference
1. Plant Physiol Biochem. 2015 Sep;94:19-27. doi: 10.1016/j.plaphy.2015.05.003. Epub 2015 May 9. Transgenic poplar expressing Arabidopsis YUCCA6 exhibits
auxin-overproduction phenotypes and increased tolerance to abiotic stress. Ke Q1, Wang Z2, Ji CY1, Jeong JC1, Lee HS1, Li H2, Xu B2, Deng X2, Kwak SS3.
2. Ke, Qingbo, et al. "Transgenic poplar expressing codA exhibits enhanced growth and abiotic stress tolerance." Plant Physiology and Biochemistry (2016)

Type Cat.No. Product Size
BIOFACT 31000-B500 20X EvaGreen™ 500 ㎕



66 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme



BioFACT™ OneStep qRT-PCR


Master Mix (For Intercalating Dye)


One-step RT-qPCR using RNA as a Template






BioFACT™ 2X Multi-Star OneStep qRT-PCR Master Mix (For Intercalating dye) is an optimized product which allows the qRT-PCR
to be done in one PCR tube by creating an enzyme mixture with Thermostable BioFACT™ RTase and H-Star(A-Star) Taq DNA poly-
merase, which has Hot Start PCR features. Intercalating dye can be selected from either SFCgreen® I or SYBR® Green I, and it can be
conveniently used simply by adding the primer and the template in order to perform PCR. This product can be used with all types
of Real-Time PCR instruments.

Feature
• High sensitivity using BioFACT™ H-Star(A-Star) Taq • Enzyme mixture of BioFACT™ RTase and
DNA polymerase H-Star(A-Star) Taq DNA Polymerase)
• Pre-denaturation time : 15 mins. • Intercalating dye : SYBR® Green I & SFCgreen® I




Application
• Quanti cation of target RNA using RT-PCR • Various applications using qPCR




QC DATA
- Comparison of sensitivity by product type (Limit of Detection)













RQ321-10h Target : Human total RNA RQ321-10h Target : Human total RNA
RQ351-10h Gene : α-CateninD RQ361-10h Gene : GAPDH
△Ct Value 50 ng 10 ng 2 ng 0.4 ng △Ct Value 50 ng 10 ng 2 ng 0.4 ng
RQ321-10h 20.29 20.10 22.5 22.48 24.87 24.72 27.44 27.42 RQ321-10h 17.56 17.59 20.05 19.84 22.31 22.18 24.68 24.61
RQ351-10h 20.25 20.26 22.43 22.49 24.7 24.89 27.27 27.27 RQ361-10h 17.46 17.56 19.84 19.86 22.3 22.25 24.81 24.68
* Comparison of sensitivity between RQ321-10h and RQ351-10h * Comparison of sensitivity between RQ321-10h and RQ361-10h
(Similar Ct values were shown when SYBR® Green I Dye was added to RQ321-10h for comparison test.) (Similar Ct values were shown when SYBR® Green I Dye was added to RQ321-10h for comparison test.)








67
BIOFACT CATALOGUE

Product Information
Master Mix Type
BioFACT ™ 2X Multi-Star OneStep qRT-PCR Master Mix ⓗincluding SYBR® Green I in mixture,
w/o Rox reference dye [RQ351-10h]

▶Contents
BioFACT ™ 2X Multi-Star OneStep qRT-PCR Master Mix
(Including SYBR® Green I in mixture)
▶Expiration date
1 Year at -20 ± 5℃







Master Mix Type
BioFACT ™ 2X Multi-Star OneStep qRT-PCR Master Mix ⓗincluding SFCgreen® I in mixture,
w/o Rox reference dye [RQ361-10h]


▶Contents
BioFACT ™ 2X Multi-Star OneStep qRT-PCR Master Mix
(Including SFCgreen® I in mixture)
▶Expiration date
1 Year at -20 ± 5℃






















Type Cat.No. Product Size
RQ351-10h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SYBR® Green I in mixture, w/o Rox reference dye 1 ㎖ x 1 ea
RQ351-50h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SYBR® Green I in mixture, w/o Rox reference dye 1 ㎖ x 5 ea
RQ353-10h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓐ including SYBR® Green I in mixture, with high Rox reference dye 1 ㎖ x 1 ea
Master Mix
RQ353-50h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓐ including SYBR® Green I in mixture, with high Rox reference dye 1 ㎖ x 5 ea
RQ361-10h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SFCgreen® I in mixture, w/o Rox reference dye 1 ㎖ x 1 ea
RQ361-50h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SFCgreen® I in mixture, w/o Rox reference dy 1 ㎖ x 5 ea







68 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme



FastFACT™ Real-Time PCR


Master Mix (For Intercalating Dye)


3X Faster than Standard Real-Time PCR Kit






FastFACT™ 2X Real-Time PCR Master Mix (For Intercalating Dye) uses the bu er and enzyme optimized for fast Real-Time PCR to
signi cantly reduce the time required for each step of PCR (denaturation, annealing, extension), from at least 2 hours to within 45
minutes.

Application
• Fast qPCR Mixture (Reaction time: within 45 mins.) • Antibody-mediated Hot Start PCR enzyme
• Low-copy transcripts amplification

QC DATA
- Comparison of activity by various template concentrations
(Comparison of amplification patterns between standard qPCR Mix and FastFACT™ Real-Time PCR Master Mix by reducing the time of each PCR step to 1 sec.)








Target : Human genomic DNA Target : Plant genomic DNA
Gene : GAPDH Gene : GAPDH

△Ct Value 50 ng 10 ng 2 ng 0.4 ng △Ct Value 50 ng 10 ng 2 ng 0.4 ng
BIOFACT Standard Type N/A 44.63 N/A 44.66 BIOFACT Standard Type N/A N/A N/A N/A
BIOFACT [FDQ388] 19.91 21.72 23.94 26.43 BIOFACT [FDQ388] 16.58 19.62 23.25 27.1
BIOFACT Standard Real-Time PCR Master Mix BIOFACT Standard Real-Time PCR Master Mix
BIOFACT Fast Real time PCR Master Mix[FDQ388]
BIOFACT Fast Real time PCR Master Mix[FDQ388]

Product Information
Master Mix Type (For Intercalating Dye)
FastFACT™ 2X Real-Time PCR Master Mix including SYBR® Green I [FDQ387-40h]

▶Contents
FastFACT™ 2X Real-Time PCR
Master Mix (including SYBR® Green I) [ 1 ㎖ x 4 ea]
▶Expiration date
1 Year at -20 ± 5℃


Type Cat.No. Product Size
FDQ387-40h FastFACT™ 2X Real-Time PCR Master Mix including SYBR® Green I in mixture, w/o Rox reference dye 1 ㎖ × 4 ea
Master Mix FDQ388-40h FastFACT™ 2X Real-Time PCR Master Mix including SYBR® Green I in mixture, with low Rox reference dye 1 ㎖ × 4 ea
FDQ389-40h FastFACT™ 2X Real-Time PCR Master Mix including SYBR® Green I in mixture, with high Rox reference dye 1 ㎖ × 4 ea



69
BIOFACT CATALOGUE

BioFACT™ Multi-Star Real-Time


PCR Master Mix ⓗ (For Probe)


Probe-based Real-Time PCR Master Mix used in Development of Various Molecular Diagnostics Kits






BioFACT™ 2X Multi-Star Real-Time PCR Master Mix (For Probe) is optimized PCR mixture for probe-based Real-Time PCR platforms
using sequence-speci c uorogenic probe chemistries.



Application
• Quanti cation of target DNA using Real-Time PCR
• Quanti cation of target RNA using RT-PCR
• Various applications using qPCR ; Genotyping, Diagnosis



QC DATA

- Example of usage in various genotyping applications


[FAM] [VIC] [Cy5]



Target : Human genomic DNA
Gene : MAPK1(FAM), GAPDH(VIC), β-2M(Cy5)



- Comparison of activity in various concentrations of template between BioFACT™ 2X Multi-Star Real-Time PCR Master Mix
(For Probe) and other Real-Time PCR Master mixes from other companies


BIOFACT Company A






△Ct Value 50 ng 10 ng 2 ng 0.4 ng
BIOFACT 22.35 24.78 27.00 29.51
Company B Company C
Company A 23.36 25.84 27.59 30.08
Company B 24.65 26.95 29.18 31.62
Company C 22.73 25.23 27.48 29.84
Target : Human genomic DNA
Gene : MAPK1(FAM)





70 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme








Product Information
Master Mix Type (For Probe)
BioFACT™ 2X Multi-Star Real-Time PCR Master Mix ⓗ [DQ322-25h]



▶Contents
2X Multi-Star Real-Time PCR Master Mix (For Porbe) [ 0.5 ㎖ x 5 ea]
▶Expiration date
1 Year and 6 months at -20 ± 5℃









































Type Cat.No. Product Size
DQ322-25h BioFACT™ 2X Multi-Star Real-Time PCR Master Mix ⓗ (For Probe) 0.5 ㎖ x 5 ea
Master Mix DQ325-25h BioFACT™ 2X Multi-Star Real-Time PCR Master Mix ⓗ (For Probe), with low Rox reference dye 0.5 ㎖ x 5 ea
DQ342-25h BioFACT™ 2X Multi-Star Real-Time PCR Master Mix ⓗ (For Probe, UDG system) 0.5 ㎖ x 5 ea
DQ442L-096(F) BioFACT™ 2X Multi-Star Real-Time PCR Pre-Mix ⓗ (For Probe, UDG system) 96 Tubes (8 strip x 12 ea)
Pre-Mix
DQ442N-096 BioFACT™ 2X Multi-Star Real-Time PCR Pre-Mix ⓗ (For Probe, UDG system) 96 Tubes (8 strip x 12 ea)
Please contact us in case of products containing ROX dye.





71
BIOFACT CATALOGUE

BioFACT™ OneStep Multi-Star


qRT-PCR Series ⓗ (For Probe)


One-step RT-qPCR using RNA as a Template






BioFACT™ OneStep Multi-Star qRT-PCR Kit (For Probe) is designed to perform one-step RT-qPCR in a single tube combining
BioFACT™ RTase and BioFACT™ 2X Multi-Star Real-Time PCR Master Mix (for Probe). It is optimized PCR mixture for probe-based
Real-Time PCR platforms using sequence-speci c probe method and it is available for use on any Real-Time PCR instruments.



Feature
• High sensitivity • Use of any sequence-speci c probe
• Easy sample optimization • OneStep qRT-PCR
• Low copy transcripts ampli cation



Application
• Quanti cation of target RNA using RT-PCR • Various applications using qPCR




QC DATA

- Comparison of sensitivity in various concentrations of template DNA between BioFACT™ OneStep Multi-star qRT PCR Kit
(For probe) and the other company's Onestep RT-qPCR Kit (Limit of Detection)

[GAPDH]

BIOFACT Company A [GAPDH] Target : Human total RNA
△Ct Value 100 ng 20 ng 4 ng 0.8 ng
BIOFACT 18.40 21.43 25.70 29.38
Company A 19.66 23.27 26.42 30.39




[MAPK1]

[MAPK1] Target : Human total RNA
BIOFACT Company A
△Ct Value 100 ng 20 ng 4 ng 0.8 ng
BIOFACT 20.90 23.61 26.09 29.54
Company A 21.35 24.31 26.84 30.87










72 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme





Product Information
Standard Type (For Probe)
BioFACT™ OneStep Multi-Star qRT-PCR Kit ⓗ [RQ811-050]


▶Contents
BioFACT™ OneStep Multi-Star qRT-PCR Enzyme Mix
5X OneStep qRT-PCR Reaction Buffer (including dNTP Mixure)
RNase-free Water
▶Expiration date
1 Year at -20±5℃





Master Mix Type (For Probe)
BioFACT™ 2X OneStep qRT-PCR Master Mix ⓗ for Probe [RQ321-10h]


▶Contents
BioFACT™ 2X OneStep qRT-PCR Master Mix (For Probe)
▶Expiration date
1 Year at -20±5℃






Master Mix Type (For Probe)
BioFACT™ 2X OneStep qRT-PCR Master Mix ⓐ for Probe [RQ341-10h]


▶Contents
BioFACT™ 2X OneStep qRT-PCR Master Mix (For Probe)
▶Expiration date
1 Year at -20±5℃












Type Cat.No. Product Size
RQ811-050 BioFACT™ OneStep Multi-Star qRT PCR Kit ⓗ (For Probe) 50 reaction
Standard
RQ811-100 BioFACT™ OneStep Multi-Star qRT PCR Kit ⓗ (For Probe) 100 reaction
RQ321-10h BioFACT™ 2X OneStep qRT-PCR Master Mix ⓗ (For probe) 1 ㎖ x 1 ea
Master Mix RQ321-50h BioFACT™ 2X OneStep qRT-PCR Master Mix ⓗ (For probe) 1 ㎖ x 5 ea
RQ341-10h BioFACT™ 2X OneStep qRT-PCR Master Mix ⓐ (For probe) 1 ㎖ x 1 ea




73
BIOFACT CATALOGUE

FastFACT™


qPCR Master Mix (For Probe)


3X Faster than Standard qPCR






FastFACT™ 2X qPCR Master Mix (For Probe) uses the bu er and enzyme optimized for Fast qPCR to signi cantly reduce the time required
for each step of PCR (denaturation, annealing, extension), from at least 2 hours to within 45 minutes. In addition, it allows multiplex am-
pli cation, in which multiple probes can be detected simultaneously.

Feature
• Fast qPCR Mixture (Reaction time: within 45 mins.)
• Antibody-mediated Hot Start PCR enzyme
• Low-copy transcripts amplification
QC DATA
- Comparison of template concentration activity between FastFACT™ 2X qPCR Master Mix (For Probe) and other company’s qPCR Master Mix



BIOFACT Company A
adenovirus adenovirus
Sample : Viral DNA (adenovirus, bocavirus)
Gene △Ct Value 10 6 copies 10 5 copies 10 4 copies 10 3 copies
adeno BIOFACT 16.62 20.71 23.28 26.41
virus Company A 17.84 20.63 23.83 N/A
boca BIOFACT 17.50 21.50 24.82 27.81
virus Company A 17.83 21.10 24.51 N/A
BIOFACT Company A
bocavirus bocavirus






Product Information
Master Mix Type (For Probe)
FastFACT™ 2X qPCR Master Mix For Probe [FDQ301-25h]


▶Contents
FastFACT™ 2X qPCR Master Mix (For Probe) [0.5 ㎖ X 5 ea]
▶Expiration date
1 Year and 6 months at -20 ± 5℃






Type Cat.No. Product Size
Master Mix FDQ301-25h FastFACT™ 2X qPCR Master Mix (For Probe) 0.5 ㎖ X 5 ea



74 BIOFACT CATALOGUE

DNA / RNA Amplification Real-Time PCR Enzyme



FastFACT™


qRT-PCR Master Mix (For Probe)


3X Faster than Standard One-step RT-qPCR






FastFACT™ 2X qRT-PCR Master Mix (For Probe) is designed to shorten the time so that One-step RT-qPCR step can be done in 40
minutes instead of over 2 hours when using standard qRT-PCR step. Enzyme and bu er of it is optimized for Fast qRT-PCR ampli-
cation by signi cantly reducing each PCR step (denaturation, annealing and extension time). Multiple probes can be detected
through its Multiplex ampli cation technology.

Feature
• Fast cDNA synthesis & qPCR (Total within 1 hour) • Optimized mixture for sequence specific probe
• Thermostable BioFACT™ RTase and H-Star Taq (Hot Start) • Quantification of target RNA using Real-Time PCR

QC DATA
- Comparison of amplification efficiency according to various template concentrations between
FastFACT™ 2X qRT-PCR Master Mix and other company’s qRT-PCR Mater Mix



BIOFACT company
ORF1ab
ORF1ab
Sample : Viral RNA (COVID-19 virus)
Gene △Ct Value 10 6 copies 10 5 copies 10 4 copies 10 3 copies
BIOFACT 17.10 21.82 24.29 27.75
ORF1ab
company A 18.76 21.21 24.12 29.76
BIOFACT 18.33 22.00 25.90 29.34
N
company A 18.70 21.94 25.24 33.95
BIOFACT company A
N N




Product Information
Master Mix Type (For Probe)
FastFACT™ 2X qRT-PCR Master Mix For Probe [FRQ301-10h]


▶Contents
FastFACT™ 2X qRT-PCR Master Mix (For Probe) [1 ㎖ X 1 ea]
▶Expiration date
1 Year at -20±5℃



Type Cat.No. Product Size
FRQ301-10h FastFACT™ 2X qRT-PCR Master Mix (For Probe) (UDG System) 1 ㎖ X 1 ea
Master Mix
FRQ301-50h FastFACT™ 2X qRT-PCR Master Mix (For Probe) (UDG System) 1 ㎖ X 5 ea



75
BIOFACT CATALOGUE

DNA / RNA



Amplification




Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.

Description 18

Lyophilized PCR Enzyme

LyoFACT™ PCR Pre-Mix Lyophilisates 40
LyoFACT™ RT Pre-Mix Lyophilisates 42
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates 44
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (For Probe) 46
LyoFACT™ All in one PCR Pre-Mix Lyophilisates 48

Real-Time PCR Enzyme
Intercalating Dye BioFACT™ Real-Time PCR Series 56
SFCgreen® I 57
BioFACT™ Real-Time PCR Series (With SFCgreen® I) 59
BioFACT™ Real-Time PCR Series (With SYBR® Green I) 64
BioFACT™ Real-Time PCR Series (With EvaGreen™) 65
20X EvaGreen™ 66
BioFACT™ OneStep qRT-PCR Master Mix 67
FastFACT™ Real-Time PCR Master Mix 69
Probe BioFACT™ Multi-Star Real-Time PCR Master Mix 70
BioFACT™ OneStep Multi-Star qRT-PCR Series 72
FastFACT™ qPCR Master Mix 74
FastFACT™ qRT-PCR Master Mix 75

PCR Enzyme

Universal PCR BioFACT™ F-Star Taq DNA Polymerase (Hot Start) 78
(<3 kb, 5 kb) BioFACT™ Thumb Taq DNA Polymerase 80
BioFACT™ Taq DNA Polymerase 82
Gene Speci c PCR BioFACT™ H-Star Taq DNA Polymerase (Hot Start) 84
(<1 kb, 5 kb) BioFACT™ A-Star Taq DNA Polymerase (Hot Start) 86

Long PCR BioFACT™ Lamp Taq DNA Polymerase 88
(<40 kb, λ DNA 기준) BioFACT™ A-Star Lamp Taq DNA Polymerase (Hot Start) 90
High Fidelity PCR BioFACT™ Pfu DNA Polymerase 92
(<3 kb, <20 kb) BioFACT™ Lamp Pfu DNA Polymerase 94
Multiplex PCR (<1 kb BioFACT™ 2X Multi-Star PCR Series (Hot Start) 96
PCR Inhibition SimpleWay™ 2X PCR Master Mix III 98


RT & RT-PCR Enzyme
BioFACT™ RT Series 102
BioFACT™ OneStep RT-PCR Series 104

Isothermal Amplification

BioFACT™ Bst DNA Polymerase 108

Other Reagent
BioFACT™ Uracil DNA Glycosylase (UDG) 112
BioFACT™ dNTP and dNTP Set 113
5X Band Helper™ 114
BioFACT™ RNase Inhibitor 116

BioFACT™


F-Star Taq DNA Polymerase


Optimal Taq DNA Polymerase for Less Than 3kb Speci c PCR






BioFACT™ F-Star Taq DNA Polymerase is designed to be highly speci c to the target gene through chemical-mediated Hot Start
system. It has similar features with BioFACT™ H-Star Taq DNA Polymerase and especially it is suitable to amplify DNA fragments larger
than 1kb. In case of chemical-mediated Hot Start PCR enzymes, its ampli cation e ciency and productivity may be lower than gen-
eral Taq DNA Polymerase. In this case, it can be supplemented by increasing the cycle.


Feature
• Source : Thermus aquaticus • A-tailing : Yes
• 5' → 3' exonuclease activity : Yes • Hot Start activity (Chemical-mediated Hot Start)
• 3' → 5' exonuclease activity ( delity) : No • All the advantages of Hot Start PCR enzyme and general Taq DNA Polymerase
• Ampli cation size : < 3 kb PCR • Error rate : 12 - 13bp error / 10 bp
6

Application
• Routine PCR • TA Cloning
• SNP / Genotyping • Suitable for UDG system





QC DATA

- Comparison of PCR activity between BioFACT™ F-Star Taq DNA Polymerase and BioFACT™ Taq DNA Polymerase Various enzyme units

BioFACT™ Taq BioFACT™ F-Star
M 1 2 3 4 5 M M 1 2 3 4 5 M
Template : Human genomic DNA
Target gene : β-globin
PCR conditions : AT 65℃, 35 cycles
M : 1kb DNA Ladder
1 : 2.5 U 2 : 1.25 U 3 : 0.62 U 4 : 0.31 U 5 : 0.15 U

- Comparison of PCR activity between BioFACT™ F-Star Taq DNA Polymerase and BioFACT™ Taq DNA Polymerase Various
concentrations of Band Helper™
BioFACT™ Taq BioFACT™ F-Star
M 1 2 3 1 2 3 M
Template : Soybean
Target gene : IFS gene, 2 Band Amplification
PCR Conditions : AT 60℃, 35 cycles
M : 1kb DNA Ladder
1 : 0X Band Helper™ 2 : 1X Band Helper™ 3 : 2X Band Helper™






78 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme





Product Information
Standard Type
BioFACT™ F-Star Taq DNA Polymerase [FS106-500]

▶Contents
BioFACT™ F-Star Taq DNA Polymerase (5 U/㎕)
10X F-Star Taq Reaction Bu er (15 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃







Master Mix Type Pre-Mix Type
BioFACT™ 2X F-Star Taq PCR Master Mix [FS301-50h] BioFACT™ F-Star Taq PCR Pre-Mix [FS401-096]











▶Contents ▶Contents
2X F-Star Taq PCR Master Mix [1 ㎖ X 5 ea] F-Star Taq PCR Pre-Mix [96 tubes]
▶Expiration date ▶Expiration date
1 Year and 6 months at -20 ± 5℃ 1 Year and 6 months at -20 ± 5℃






Reference
1. Bioresour Technol. 2015 Dec 5;202:125-132. doi: 10.1016/j.biortech.2015.11.085. [Epub ahead of print] Monitoring the microbial community shift throughout
the shock changes of hydraulic retention time in an anaerobic moving bed membrane bioreactor. Win TT1, Kim H2, Cho K2, Song KG3, Park J4





Type Cat.No. Product Size
FS106-500 BioFACT™ F- Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.4 ㎖) 500 U
Standard FS106-25h BioFACT™ F- Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 2 ㎖) 2,500 U (500 U x 5 ea)
FS106-50h BioFACT™ F- Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 4 ㎖) 5,000 U (500 U x 10 ea)
Master Mix FS301-50h BioFACT™ 2X F-Star Taq PCR Master Mix 1 ㎖ x 5 ea
FS401-096 BioFACT™ 2X F-Star Taq PCR Pre-Mix, Standard, w/o Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
Pre-Mix
FS401-480 BioFACT™ 2X F-Star Taq PCR Pre-Mix, Standard, w/o Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)
* Please Contact us about PCR Master Mix or Pre-Mix without dye.




79
BIOFACT CATALOGUE

BioFACT™


Thumb Taq DNA Polymerase


Taq DNA polymerase with Increased PCR e ciency






BioFACT™ Thumb Taq DNA Polymerase is a widely used polymerase product most commonly used in the traditional PCR with applica-
tions in general PCR such as colony PCR as well as other various types of PCR methods for diagnosis and testing. Due to high ampli ca-
tion e ciency compared to Taq DNA polymerase, a large amount of PCR products can be obtained.

Feature
• Source : Thermus aquaticus • A-tailing : Yes
• 5' → 3' exonuclease activity : Yes • Hot Start activity : No
6
• 3' → 5' exonuclease activity ( delity) : No • Error rate : 12 - 13bp error / 10 bp
• Ampli cation size : < 5 kb PCR


Application
• Routine PCR • TA Cloning
• SNP / Genotyping



QC DATA
- Comparison of enzyme activity by various enzyme units between BioFACT™ Taq and BioFACT™ Thumb Taq
< AP DNA endonuclease gene Human 1 Kb > < β-Globin gene Human 3.6 Kb >
BioFACT™ Taq BioFACT™ Thumb Taq BioFACT™ Taq BioFACT™ Thumb Taq
M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6 Human Genomic DNA
1 2 3 4 5 6
1 2 3 4 5 6
1 2 3 4 5 6
1 2 3 4 5 6
10 ng, 35 cycles
M : 1kb DNA Ladder
1: 2 unit
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 2: 1 unit
1 2 3 4 5 6
1 2 3 4 5 6
1 2 3 4 5 6
1 2 3 4 5 6
3: 0.5 unit
4: 0.25 unit
5: 0.125 unit
6: 0.0625 unit
- Comparison of amplification efficiency by various template concentrations between BioFACT™ Taq and BioFACT™ Thumb Taq
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
< AP DNA endonuclease gene Human 1 Kb > < β-Globin gene Human 3.6 Kb >
BioFACT™ Taq BioFACT™ Thumb Taq BioFACT™ Taq BioFACT™ Thumb Taq
M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6 Enzyme 0.625 unit,
35 cycles
M : 1kb DNA Ladder
1: 50 ng
2: 10 ng
3: 2 ng
4: 0.4 ng
5: 0.08 ng
6: 0.016 ng


80 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme



BioFACT™ Product Information


Thumb Taq DNA Polymerase Standard Type
BioFACT™ Thumb Taq DNA Polymerase [TT116-500]

▶Contents
BioFACT™ Thumb Taq DNA Polymerase (2.5 U/㎕)
10X Thumb Taq Reaction Bu er (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃







Master Mix Type Pre-Mix Type
BioFACT™ 2X Thumb Taq PCR Master Mix [TT301-50h] BioFACT™ 2X Thumb Taq PCR Pre-Mix [TT402-096]











▶Contents ▶Contents
2X Thumb Taq PCR Master Mix [1 ㎖ X 5 ea] 2X Thumb Taq PCR Pre-Mix [96 tubes]
▶Expiration date ▶Expiration date
1 Year and 6 months at -20 ± 5℃ 1 Year and 6 months at -20 ± 5℃






Reference
1. Bioresour Technol. 2015 Dec 5;202:125-132. doi: 10.1016/j.biortech.2015.11.085. [Epub ahead of print] Monitoring the microbial community shift throughout
the shock changes of hydraulic retention time in an anaerobic moving bed membrane bioreactor. Win TT1, Kim H2, Cho K2, Song KG3, Park J4






Type Cat.No. Product Size
Standard TT116-500 BioFACT™ Thumb Taq DNA polymerase with 10 mM dNTP Mix (each 10 mM, 0.4 ㎖) 500 U
TT301-50h BioFACT™ Thumb Taq PCR Master Mix 1, w/o Band Helper™, with dye 1 ㎖ × 5 ea
Master Mix
TT302-50h BioFACT™ Thumb Taq PCR Master Mix 2 with 0.5X Band Helper™, with dye 1 ㎖ × 5 ea
TT402-096 BioFACT™ Thumb Taq PCR Pre-Mix, with 0.5X Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
Pre-Mix
TT402-480 BioFACT™ Thumb Taq PCR Pre-Mix, with 0.5X Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)
* Please Contact us about PCR Master Mix or Pre-Mix without dye.




81
BIOFACT CATALOGUE

BioFACT™


Taq DNA Polymerase


The Most Common PCR Enzyme, Taq DNA Polymerase






BioFACT™ Taq DNA Polymerase is a thermostable DNA polymerase from Thermus aquaticus that possesses 5' to 3' polymerase activity. It
is the enzyme most widely used in various PCR-based techniques from routine PCR applications such as genotyping, RT-PCR for diagnosis
and colony PCR to demanding PCR applications.

Feature
• Source : Thermus aquaticus • Hot Start activity : No
• 5' → 3' exonuclease activity : Yes • A-tailing : Yes
• 3' → 5' exonuclease activity ( delity) : No • Error rate : 12 - 13 bp error / 10 bp
6
• Ampli cation size : < 5 kb PCR

Application
• Routine PCR • Colony PCR
• PCR for diagnosis • Suitable for UDG System
• TA Cloning



QC DATA
- Comparison of amplification efficiency according to various PCR fragment sizes

BIOFACT Company A Company B Company C Company D
M A B C D E M A B C D E M A B C D E M A B C D E M A B C D E M
Target : Human genomic DNA 50 ng
Gene : β-Globin
PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder
A : 0.5 kb B : 1 kb C : 2 kb D : 3.6 kb E : 5 kb

- Comparison of amplification efficiency according - Comparison of activity test according
to various template concentrations to various enzyme units
M A B C D E F M M A B C D E M
BIOFACT BIOFACT
Company A Company A

Company B Company B

Company C Company C
Company D
Company D
Target : Human genomic DNA 10 ng Target : Human genomic DNA 10 ng
PCR Condition : 60°C, 35 cycles PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng A : 2.5U B : 1.25U C : 0.625U D : 0.313U E : 0.156U



82 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme





Product Information
Standard Type
BioFACT™ Taq DNA Polymerase [ST116-500]

▶Contents
BioFACT™ Taq DNA Polymerase (5 U/㎕)
10X Taq Reaction Buffer (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃






Master Mix Type Pre-Mix Type
BioFACT™ 2X Taq PCR Master Mix [ST301-50h] BioFACT™ 2X Taq PCR Pre-Mix [ST402-096]













▶Contents ▶Contents
2X Taq PCR Master Mix [1 ㎖ x 5 ea] 2X Taq PCR Pre-Mix [96 tubes]
▶Expiration date ▶Expiration date
1 Year and 6 months at -20 ± 5℃ 1 Year and 6 months at -20 ± 5℃





Reference
1. J Biotechnol. 2015 Aug 20;208:1-10. doi: 10.1016/ j.jbiotec.2015.05.006. Epub 2015 May 23. Production of 13S-hydroxy-9(Z)-octadecenoic acid from linoleic acid
by whole recombinant cells expressing linoleate 13-hydratase from Lactobacillus acidophilus. Park JY1, Lee SH1, Kim KR1, Park JB2, Oh DK3.




Type Cat.No. Product Size
ST116-500 BioFACT™ Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.4㎖) 500 U
ST116-25h BioFACT™ Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 2㎖) 2,500 U (500 U x 5 ea)
Standard ST116-50h BioFACT™ Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 4㎖) 5,000 U (500 U x 10 ea)
ST166-500 BioFACT™ Taq DNA Polymerase with 10mM dNTP(U) Mix (each 10mM, 0.4㎖) 500 U
ST111-500 BioFACT™ Taq DNA Polymerase, w/o dNTP, w/o Band Helper™ 500 U
ST301-50h BioFACT™ 2X Taq PCR Master Mix 1, w/o Band Helper™, with dye 1 ㎖ x 5 ea
Master Mix
ST302-50h BioFACT™ 2X Taq PCR Master Mix 2, with 0.5X Band Helper™, with dye 1 ㎖ x 5 ea
ST402-096 BioFACT™ 2X Taq PCR Pre-Mix, with Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
Pre-Mix
ST402-480 BioFACT™ 2X Taq PCR Pre-Mix, with Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)





83
BIOFACT CATALOGUE

BioFACT™


H-Star Taq DNA Polymerase


Highly Speci c Ampli cation of Less Than 1 kb Size Target DNA






BioFACT™ H-Star Taq DNA Polymerase is a chemical-mediated Hot Start Taq DNA polymerase which leads to complete inactivation of
the polymerase until the initial heat activation step. Through this feature, it provides high PCR speci city, avoiding extension of non
speci c annealed primers or primer-dimers and it is highly suitable for e cient ampli cation of di cult templates caused by high GC
content or complex structure. BioFACT™ H-Star Taq DNA Polymerase is optimized to amplify less than 1 kb size target DNA and it can
be supplemented by increasing the cycle as ampli cation e ciency and productivity of chemical-mediated Hot Start PCR enzymes
may be lower than general Taq DNA Polymerase.
Feature
• Source : Thermus aquaticus • Hot Start activity (Chemical-mediated Hot Start)
• 5' → 3' exonuclease activity : Yes • A-tailing : Yes
• 3' → 5' exonuclease activity ( delity) : No • Error rate : 12 - 13 bp error / 10 bp
6
• Ampli cation size : < 1 kb PCR

Application
• Hot Start PCR • TA cloning • High speci city PCR
• Real-Time PCR • Multiplex PCR • Allele speci c PCR
• High G+C rich / Repeat region PCR • Genotyping • Suitable for UDG System

QC DATA
- Comparison of Hot Start PCR enzyme activity between - Comparison of amplification efficiency according to
BioFACT™ H-Star Taq DNA Polymerase and other various template concentrations
companies' Hot Start PCR enzymes

ApoE (0.9 kb) APEX (1 kb) ApoE (0.9 kb) APEX (1 kb)
M A B C D E F M M A B C D E F M M A B C D E F M M A B C D E F M
BIOFACT BIOFACT

Company A Company A
Company B Company B

Company C Company C
Target : Human genomic DNA 10 ng PCR Condition : 60°C, 35 cycles Target : Human genomic DNA PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 2.5 U B : 1.25 U C : 0.625 U D : 0.313 U E : 0.156 U F : 0.078 U A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng


- Amplification data of various target genes using H-Star Taq at fixed annealing temperature



Various DNA detection at fixed annealing temperature (55℃)
PCR was carried out at the same annealing temperature condition using human
genomic DNA 100 ng/㎕ and 80 kinds of primer sets.



84 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme





Product Information
Standard Type
BioFACT™ H-Star Taq DNA Polymerase [HS106-250]

▶Contents
BioFACT™ H-Star Taq DNA Polymerase (2.5 U/㎕)
10X H-Star Taq Reaction Buffer (15 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃






Master Mix Type Pre-Mix Type
BioFACT™ 2X H-Star Taq PCR Master Mix [HS301-40h] BioFACT™ 2X H-Star Taq PCR Pre-Mix [HS401-096]













▶Contents ▶Contents
2X H-Star Taq PCR Master Mix [1 ㎖ x 4 ea] 2X H-Star Taq PCR Pre-Mix [96 tubes]
▶Expiration date ▶Expiration date
1 Year and 6 months at -20 ± 5℃ 1 Year and 6 months at -20 ± 5℃













Type Cat.No. Product Size
HS106-250 BioFACT™ H-Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.2 ㎖) 250 U
Standard
HS106-10h BioFACT™ H-Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.8 ㎖) 1,000 U (250 U x 4 ea)
HS301-40h BioFACT™ 2X H-Star Taq PCR Master Mix 1, w/o Band Helper™ , with dye 1 ㎖ × 4 ea
Master Mix HS303-40h BioFACT™ 2X H-Star Taq PCR Master Mix 2, with 1X Band Helper™ , with dye 1 ㎖ × 4 ea
HS341-40h BioFACT™ 2X H-Star Taq PCR Master Mix 1, w/o Band Helper™, with dye (UDG System) 1 ㎖ × 4 ea
HS401-096 BioFACT™ 2X H-Star Taq PCR Pre-Mix, w/o Band Helper™,with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
Pre-Mix
HS401-480 BioFACT™ 2X H-Star Taq PCR Pre-Mix, w/o Band Helper™,with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)
* Please Contact us about PCR Master Mix or Pre-Mix without dye.





85
BIOFACT CATALOGUE

BioFACT™


A-Star Taq DNA Polymerase


Antibody-Mediated Hot Start PCR Enzyme






BioFACT™ A-Star Taq DNA Polymerase, isolated from Thermus aquaticus and expressed in E.coli through a simple and modi ed puri ca-
tion procedure, is Hot Start PCR enzyme which is combined with anti-Taq monoclonal antibody that inhibits polymerase activity until it
is heat denatured. It has shorter pre-denaturation time than chemical-modi ed Hot Start PCR enzyme and it also prevents non speci c
ampli cation and primer-dimer formation. BioFACT™ A-Star Taq DNA Polymerase is highly suitable for ampli cation reactions involving
low-copy targets, complex genomic DNA or cDNA templates. Providing optional 5X Band Helper™ helps ampli cation of di cult PCR
caused by high GC content or complex structure of template.




Feature
• Source : Thermus aquaticus • Hot Start activity (Antibody-mediated Hot Start) : Yes
• 5' → 3' exonuclease activity : Yes • All the advantages of Hot Start PCR enzyme and general Taq DNA Polymerase
• 3' → 5' exonuclease activity ( delity) : No • High speci city & minimization of primer-dimer formation
• Ampli cation size : < 5 kb PCR • High activity & high sensitivity
• A-tailing : Yes • Easy PCR optimization



Application
• Routine PCR • Colony PCR
• PCR for diagnosis • Suitable for UDG system
• TA cloning

QC DATA

- Comparison of amplification efficiency according to various - Comparison of amplification efficiency according to vari-
enzyme units between BioFACT™ A-Star Taq DNA Polymerase ous template concentrations between BioFACT™ A-Star
and BioFACT™ Taq DNA Polymerase Taq DNA Polymerase and BioFACT™ Taq DNA Polymerase

Taq A-Star Taq Taq A-Star Taq
M A B C D E F M A B C D E F M A B C D E F M A B C D E F
NF-κ B NF-κ B
(1.2 kb) (1.2 kb)

TNFR1 TNFR1

Target : Human genomic DNA 10 ng PCR Condition : 60℃, 35 cycles Target : Human genomic DNA PCR Condition : 60℃, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng







86 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme



BioFACT™


A-Star Taq DNA Polymerase - Comparison of gradient PCR data between BioFACT™ A-Star Taq DNA Polymerase and BioFACT™ Taq DNA Polymerase

Taq A-Star Taq
M 1 2 3 4 5 6 7 8 9 10 11 12 M M 1 2 3 4 5 6 7 8 9 10 11 12 M









Target : Human genomic DNA 10 ng, NF-κ B(1.2 kb) PCR Condition : 35 cycles M : 1kb DNA Ladder, 1~12 Lane : Annealing Temperature
1 : 50.0℃ 2 : 50.3℃ 3: 51.2℃ 4 : 52.7℃ 5 : 54.7℃ 6 : 57.2℃ 7 : 60.2℃ 8 : 62.9℃ 9: 64.9℃ 10 : 66.6℃ 11 : 67.5℃ 12 : 68.0℃







Product Information
Standard Type
BioFACT™ A-Star Taq DNA Polymerase [AT116-500]

▶Contents
BioFACT™ A-Star Taq DNA Polymerase (5 U/㎕)
10X A-Star Taq Reaction Buffer (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃





















Type Cat.No. Product Size
AT116-500 BioFACT™ A-Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.4 ㎖) 500 U
Standard AT116-25h BioFACT™ A-Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 2 ㎖) 2,500 U (500 U x 5 ea)
AT116-50h BioFACT™ A-Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 4 ㎖) 5,000 U (500 U x 10 ea)





87
BIOFACT CATALOGUE

BioFACT™


Lamp Taq DNA Polymerase


Accurate Long Range PCR with Low Error Rate






BioFACT™ Lamp Taq DNA Polymerase contains a blend of Taq DNA Polymerase and Pfu DNA polymerase which exhibits a 3' → 5'
exonuclease activity (proofreading). It is highly suitable for PCR ampli cation of long DNA fragments from 5 to 20 kb and it exhibits
low error rate due to its proofreading activity (week). BioFACT™ Lamp Taq DNA Polymerase provides increased and higher yields than
that which can be achieved with Taq DNA polymerase through its superior ampli cation e ciency and it generates PCR products with
A-tailing, used for TA cloning.


Feature
• Source : Pyrococcus furiosus & Thermus aquaticus • Hot Start activity: No
• 5' → 3' exonuclease activity : Yes • A-tailing : Yes
• 3' → 5' exonuclease activity ( delity) : Yes (weak) • Error rate : 5 - 6 bp error/ 10 bp
6
• Ampli cation size : <40 kb, based on λDNA



Application
• Long range PCR (<40 kb, based on λDNA) • TA cloning
• High productivity PCR • Colony PCR
• Inverse PCR




QC DATA

- PCR amplification data for various gene sizes between BioFACT™ Lamp Taq DNA Polymerase and other companies' PCR enzymes

BIOFACT Company A Company B Company C
M A B C D E F G M M A B C D E F G M M A B C D E F G M M A B C D E F G M













Template : Lambda DNA PCR Condition : AT 68℃, 30 Cycles
M : 1 kb Ladder A : 1 kb B : 3.6 kb C : 5 kb D : 10 kb E : 14 kb F : 17 kb G : 22 kb





88 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme





Product Information
Standard Type
BioFACT™ Lamp Taq DNA Polymerase [LT116-250]


▶Contents
BioFACT™ Lamp Taq DNA Polymerase (2.5 U/㎕)
10X Lamp Taq Reaction Buffer (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃






Master Mix Type Pre-Mix Type
BioFACT™ 2X Lamp Taq PCR Master Mix [LT301-50h] BioFACT™ 2X Lamp Taq PCR Pre-Mix [LT402-096]













▶Contents ▶Contents
2X Lamp Taq PCR Master Mix [1 ㎖ x 5ea] 2X Lamp Taq PCR Pre-Mix [96 tubes]

▶Expiration date ▶Expiration date
1 Year and 6 months at -20 ± 5℃ 1 Year and 6 months at -20 ± 5℃









Type Cat.No. Product Size
LT116-250 BioFACT™ Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.3 ㎖) 250 U
LT116-500 BioFACT™ Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.6 ㎖) 500 U
Standard
LT116-25h BioFACT™ Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 3 ㎖) 2,500 U (500 U × 5 ea)
LT116-50h BioFACT™ Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 6 ㎖) 5,000 U (500 U × 10 ea)
LT301-50h BioFACT™ 2X Lamp Taq PCR Master Mix 1, w/o Band Helper™, with dye 1 ㎖ × 5 ea
Master Mix
LT302-50h BioFACT™ 2X Lamp Taq PCR Master Mix 2, with 0.5X Band Helper™, with dye 1 ㎖ × 5 ea
LT402-096 BioFACT™ 2X Lamp Taq PCR Pre-Mix, with 0.5X Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
Pre-Mix
LT402-480 BioFACT™ 2X Lamp Taq PCR Pre-Mix, with 0.5X Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)
* Please Contact us about PCR Master Mix or Pre-Mix without dye.




89
BIOFACT CATALOGUE

BioFACT™ A-Star


Lamp Taq DNA Polymerase


Antibody-Mediated Hot Start PCR Enzyme for Long Range PCR






BioFACT™ A-Star Lamp Taq DNA Polymerase is a blended enzyme of Taq DNA polymerase and Pfu DNA Polymerase processing proofread-
ing capability. It o ers the speci city and sensitivity of Hot Start polymerase through anti-Taq monoclonal antibody that inactivates the
enzyme until the rst denaturation step, avoiding extension of non-speci c annealed primers or primer-dimers.
BioFACT™ A-Star Lamp Taq DNA Polymerase is highly suitable for e cient ampli cation of di cult templates caused by high GC content
or complex structure and it is especially ideal for accurately amplifying long fragments with high yields and speci city.


Feature
• Source : Pyrococcus furiosus & Thermus aquaticus • Hot Start activity (Antibody-mediated Hot Start) : Yes
• 5'→ 3' exonuclease activity : Yes • Long range PCR Enzyme
• 3'→ 5' exonuclease activity ( delity) : Yes (weak) • High sensitivity & high speci city
• Ampli cation size : <40 kb, based on λDNA • Antibody mediated Hot Start PCR enzyme
• A-tailing : Yes • Facilitation of low copy DNA detection


Application
• Long range PCR (<40 kb, based on λDNA) • TA cloning
• Di cult PCR • Colony PCR
• High productivity PCR • Suitable for UDG system
• Inverse PCR



QC DATA
- Comparison of LOD according to various enzyme units - Comparison of LOD according to various template
between BioFACT™ A-Star Lamp Taq DNA Polymerase concentrations between BioFACT™ A-Star Lamp Taq
and the other company's PCR enzyme DNA Polymerase and the other company's PCR enzyme
BIOFACT Company A BIOFACT Company A
M A B C D E M M A B C D E M M A B C D E M M A B C D E M
APEX (1 Kb) APEX (1 Kb)

TNFR1 (722 bp)
TNFR1 (722 bp)
p53 (1.1 Kb)
GLO (1 Kb)
GLO (1 Kb)
GLO (5 Kb)
GLO (5 Kb)
Target : Human genomic DNA 50 ng PCR Condition : 60°C, 35 cycles Target : Human genomic DNA 50 ng PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 2.5 U B : 1.25 U C : 0.625 U D : 0.313 U E : 0.156 U A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng




90 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme







- Comparison of long PCR amplification efficiency between BioFACT™ A-Star Lamp Taq DNA Polymerase and the other
company's PCR enzyme

BIOFACT Company A
M 1 2 3 4 5 6 M 1 2 3 4 5 6 M





Target : Human genomic DNA 50 ng
PCR Condition : 68°C, 30 cycles
M : 1kb DNA Ladder
1 : 0.55 kb 2 : 1 kb 3 : 2 kb 4 : 3 kb 5 : 5 kb 6 : 10 kb





Product Information
Standard Type
BioFACT™ A-Star Lamp Taq DNA Polymerase [AL116-250]

▶Contents
BioFACT™ A-Star LampTaq DNA Polymerase (2.5 U/㎕)
10X A-Star LampTaq Reaction Buffer (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃






















Type Cat.No. Product Size
AL116-250 BioFACT™ A-Star Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.3 ㎖) 250 U
Standard AL116-500 BioFACT™ A-Star Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.6 ㎖) 500 U
AL116-25h BioFACT™ A-Star Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 3 ㎖) 2,500 U (500 U x 5 ea)
Please Contact us about PCR Master Mix or Pre-Mix without dye.




91
BIOFACT CATALOGUE

BioFACT™


Pfu DNA Polymerase


High Fidelity / Ampli cation of Blunt-End Fragment






BioFACT™ Pfu DNA Polymerase is recombinant enzyme derived from Pyrococcus furiosus and expressed in E.coli. It has superior ther-
mostability and possesses 3' to 5' exonuclease proofreading activity which results in PCR fragments with fewer errors. PCR fragments
generated by BioFACT™ Pfu DNA Polymerase are blunt-ended and it can be available for blunt end cloning using All in One™ PCR Cloning
Kit without additional A-tailing step.


Feature
• Source : Pyrococcus furiosus • A-tailing : No
6
• 5' → 3' exonuclease activity : No • Error rate : 1-2 bp error / 10 bp
• 3' → 5' exonuclease activity ( delity) : Yes • 12-fold higher PCR delity over Taq DNA Polymerases
• Ampli cation size : < 3 kb PCR

Application
• High delity PCR • Blunt end form cloning
• Site-directed mutagenesis


QC DATA
- Comparison of amplification efficiency according to various sizes of template between BioFACT™ Pfu DNA Polymerase and
other companies' Pfu DNA Polymerases

BioFACT™ Pfu Company A Company B
M 1 2 3 4 5 6 7 M 1 2 3 4 5 6 7 M 1 2 3 4 5 6 7

Template : Human genomic DNA, Corynebacterium Ch's phage λ DNA
PCR Conditions : AT 68℃ (3, 4 : 62℃) 30 cycles
M : 1kb DNA Ladder
1 : 0.5 kb 2 : 1 kb 3 : 1.5 kb 4 : 3 kb 5 : 4 kb 6 : 6 kb 7 : 10 kb


- Comparison of fidelity for various PCR enzymes

10
DNA polymerase Average Mutation Frequency* Average Error Rate** Relative Fidelity
(%±SD)
±SD)
(10 -6
Enzyme Accuracy (x10 5 ) 6 4 Lamp Taq 4.03±1.43 23.0±19.0 10x
8
Taq
1x
2.31±2.2
0.45±0.73
Pfu
2 Lamp Pfu 0.16±0.05 0.96±0.4 24x
1.22±0.9
0.21±0.07
19x
0 • The average of five independent rpsL Fidelity Assays is shown.
Taq Lamp Taq Pfu Lamp Pfu
*Mutation Frequencies were calculated using the equation : MF = (Apr Smr colonies) / [Apr colonies × dilution factor × 100%].
Error rate = mutation / bp / duplication **Error Rates were calculated using the equation : ER = Mutation Frequency / (Template Doublings × 130 bp).
• Template Doublings were determined using the equation : TD = (amount of PCR product) / (amount of starting product).
• Template Doublings for all three polymerases ranged from 11 to 12 for 25 PCR cycles, and 6 to 7 for 15 cycles.
92 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme





Product Information
Standard Type
BioFACT™ Pfu DNA Polymerase [PD116-250]



▶Contents
BioFACT™ Pfu DNA Polymerase (2.5 U/㎕)
10X Pfu Reaction Buffer (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃






Master Mix Type Pre-Mix Type
BioFACT™ 2X Pfu PCR Master Mix [PD301-50h] BioFACT™ 2X Pfu PCR Pre-Mix [PD401-096]












▶Contents ▶Contents
2X Pfu PCR Master Mix [1 ㎖ x 5 ea] 2X Pfu PCR Pre-Mix [96 tubes]
▶Expiration date ▶Expiration date
1 Year and 6 months at -20 ± 5℃ 1 Year and 6 months at -20 ± 5℃









Type Cat.No. Product Size
PD116-250 BioFACT™ Pfu DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.3 ㎖) 250 U
PD116-500 BioFACT™ Pfu DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.6 ㎖) 500 U
Standard
PD116-25h BioFACT™ Pfu DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 3 ㎖) 2,500 U (500 U × 5 ea)
PD116-50h BioFACT™ Pfu DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 6 ㎖) 5,000 U (500 U × 10 ea)
PD301-50h BioFACT™ 2X Pfu PCR Master Mix 1, w/o Band Helper™, with dye 1 ㎖ × 5 ea
Master Mix
PD302-50h BioFACT™ 2X Pfu PCR Master Mix 2, with 0.5X Band Helper™, with dye 1 ㎖ × 5 ea
PD401-096 BioFACT™2X Pfu PCR Pre-Mix, w/o Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
Pre-Mix
PD401-480 BioFACT™2X Pfu PCR Pre-Mix, w/o Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)
* Please Contact us about PCR Master Mix or Pre-Mix without dye.





93
BIOFACT CATALOGUE

BioFACT™


Lamp Pfu DNA Polymerase


Blunt-End Fragment Ampli cation of Less than 20 kb with High Accuracy






BioFACT™ Lamp Pfu DNA Polymerase is an upgraded version of existing Pfu DNA polymerase using mutagenesis to express the Pyro-
coccus furiosus DNA polymerase gene in E.coli. While retaining the features of BioFACT™ Pfu DNA Polymerase, the extension time is
signi cantly reduced to 30 seconds per 1kb and the ampli cation rate is much better than that of Pfu DNA Polymerase.
In addition, it enables the ampli cation of long PCR fragments of 20 kb (based on human gDNA). This enzyme also allows blunt
cloning without an additional A-tailing process using the All in One™ PCR Cloning Kit.


Feature
• Source : Pyrococcus furiosus • A-tailing : No
• 5' → 3' exonuclease activity : No • 4X faster processivity than Pfu DNA polymerase
• 3' → 5' exonuclease activity ( delity) : Yes • Error rate : 1-2 bp or less error/ 10 bp
6
• Ampli cation size : < 20 kb PCR

Application
• High delity PCR • Blunt end form cloning
• Site-directed mutagenesis • Long PCR (<20 kb)


QC DATA
- Comparison of activity by concentration of enzyme - Comparison of activity by concentration of template
between BioFACT™ Lamp Pfu DNA Polymerase and between BioFACT™ Lamp Pfu DNA Polymerase and
other Pfu DNA Polymerase other Pfu DNA Polymerase
BIOFACT Company A BIOFACT Company A
M A B C D E M M A B C D E M M A B C D E F M M A B C D E F M
APEX APEX
(1 kb) (1 kb)
NF-κ B NF-κ B
(1.2 kb) (1.2 kb)

SOX2 SOX2
(1 kb) (1 kb)
TNFR1 TNFR1
(0.7 kb) (0.7 kb)
p53 p53
(1.1 kb) (1.1 kb)

GLO GLO
(5 kb) (5 kb)
Target : Human genomic DNA 50 ng PCR Condition : 60°C, 35 cycles Target : Enzyme 1.25 unit PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 2.5 U B : 1.25 U C : 0.625 U D : 0.313 U E : 0.156 U A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng





94 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme



BioFACT™


Lamp Pfu DNA Polymerase - Comparison of long PCR amplification efficiency between BioFACT™ Lamp Pfu DNA Polymerase and other Pfu DNA Polymerase

BIOFACT Company A
M A B C D E F M M A B C D E F M






Template : Human gDNA (GLO Primer Set)
PCR Condition : AT 68°C, 30 Cycles
M : 1kb DNA Ladder
A : 0.5 kb B : 1 kb C : 2 kb D : 3.6 kb E : 5 kb F : 10 kb








Product Information
Standard Type
BioFACT™ Lamp Pfu DNA Polymerase [LP116-250]


▶Contents
BioFACT™ Lamp Pfu DNA Polymerase (2.5 U/㎕)
10X Lamp Pfu Reaction Buffer (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

▶Expiration date
2 Year and 3 months at -20 ± 5℃





















Type Cat.No. Product Size
LP116-250 BioFACT™ Lamp Pfu DNA polymerase with 10 mM dNTP Mix (each 10 mM, 0.3 ㎖) 250 U
Standard
LP116-500 BioFACT™ Lamp Pfu DNA polymerase with 10 mM dNTP Mix (each 10 mM, 0.6 ㎖) 500 U





95
BIOFACT CATALOGUE

BioFACT™


2X Multi-Star PCR Series


Optimal PCR Kit for Multi Band Ampli cation






Multiplex PCR is a simple, e cient and powerful PCR technique that allows simultaneous ampli cation of two or more target genes
in parallel in a single tube. BioFACT™ 2X Multi-Star PCR Series is available in a convenient ready-to-use Master Mix / Pre-Mix format
which contains Hot Start PCR enzyme and optimized bu er which minimize inhibition of di erent primer sets in same tube during
reaction. Included BioFACT™ H-Star Taq DNA Polymerase which is chemical-mediated Hot Start DNA polymerase enables high spe-
ci c and accurate detection by inhibition of non speci c ampli cation and primer-dimer due to its automatic heat-activation charac-
teristics. BioFACT™ 2X Multi-Star PCR Series is highly suitable for research laboratories, forensic, diagnostic genotyping applications
where simultaneous analysis of multiple markers is required.
Feature
• Source : Thermus aquaticus • Hot Start activity (Chemical-mediated Hot Start) : Yes
• 5' → 3' exonuclease activity : Yes • A-tailing : Yes
• 3' → 5' exonuclease activity ( delity) : No • Pre-denaturation time: 15 mins.
• Ampli cation size : < 1 kb PCR

Application
• Multiplex PCR ( ~ 13 bands in a parallel) • General sequencing
• Allele speci c PCR ( ~ 21 bands in a parallel) • TA cloning
• Methylation speci c PCR • SSR / MS
• SNP detection • Suitable for UDG system


QC DATA
- Comparison of amplification efficiency on various primer sets between BioFACT™ 2X Multi-Star PCR Series
and other companies' multiplex PCR enzymes
BIOFACT Company A
M 1 2 3 4 5 6 7 8 9 10 M M 1 2 3 4 5 6 7 8 9 10 M









Company B Company C
M 1 2 3 4 5 6 7 8 9 10 M M 1 2 3 4 5 6 7 8 9 10 M








Target : Human genomic DNA PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder Primer : 10 set 1 - 10 : various templates of genomic DNA



96 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme







- Single / multiplex PCR

[Single PCR] [Multiplex PCR]


M A B C D E F G H I J M M A B C D E F G H I J M








Template : Human gDNA (House keeping gene) Template : Human gDNA(House keeping gene)
PCR Condition : AT 60℃, 30 Cycles PCR Condition : AT 60℃, 30 Cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A~J : Gene 1 ~ Gene 10 A~J : Gene 1 ~ Gene 10 (Primer sets are steadily increased)




Product Information
Master Mix Type Pre-Mix Type
BioFACT™ 2X Multi-Star PCR Master Mix [MS301-25h] BioFACT™ 2X Multi-Star PCR Pre- Mix [MS401-096]













▶Contents ▶Contents
2X Multi-Star PCR Master Mix [0.5 ㎖ x 5 ea] 2X Multi-Star PCR Pre-Mix [96 tubes]

▶Expiration date ▶Expiration date
1 Year and 6 months at -20 ± 5℃ 1 Year and 6 months at -20 ± 5℃










Type Cat.No. Product Size
MS301-25h BioFACT™ 2X Multi-Star PCR Master Mix, with dye 0.5 ㎖ × 5 ea
Master Mix
MS302-25h BioFACT™ 2X Multi-Star PCR Master Mix, w/o dye 0.5 ㎖ × 5 ea
MS401-096 BioFACT™ 2X Multi-Star PCR Pre-Mix, with dye, total 30 ㎕ reactions 96 Tubes (8 strip × 12 ea)
Pre-Mix
MS402-096 BioFACT™ 2X Multi-Star PCR Pre-Mix, w/o dye, total 30 ㎕ reactions 96 Tubes (8 strip × 12 ea)
* Please Contact us about PCR Master Mix or Pre-Mix without dye.




97
BIOFACT CATALOGUE

SimpleWay™


2X PCR Master Mix Ⅲ


Direct PCR that Minimizes PCR Inhibitors






SimpleWay™ 2X PCR Master Mix III enables direct PCR without genomic DNA extraction by simply adding the sample itself or the
lysed sample to the Master Mix. A large amount of sample can be ampli ed conveniently and quickly with improved ampli cation
e ciency by minimizing the e ects of PCR Inhibitors in the sample using an optimized composition of bu er and enzyme.




Feature
• No genomic DNA Preparation • Low PCR inhibition
• Rapid PCR • Low handling error


Application
• PCR
• Direct PCR
• Genotyping









Product Information
Master Mix Type
SimpleWay™ 2X PCR Master Mix III [SW331-25h]


▶Contents
SimpleWay™ 2X PCR Master Mix III [0.5 ㎖ x 5 ea]
▶Expiration date
1 Year and 6 months at -20 ± 5℃






Type Cat.No. Product Size
Master Mix SW331-25h SimpleWay™ 2X PCR Master Mix III 0.5 ㎖ X 5 ea








98 BIOFACT CATALOGUE

DNA / RNA Amplification PCR Enzyme




















































































99
BIOFACT CATALOGUE

DNA / RNA



Amplification




Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.