Ligase & Electrophoresis Reagent
EcoDye™
Nucleic Acid Staining Solution
Safer Alternative to EtBr for Detecting Nucleic Acid
EcoDye™ Nucleic Acid Staining Solution is a stable and sensitive DNA/RNA uorescent dye. It is Alternatives to EtBr non-mutagenic
and non-cytotoxic and can be used instead of Ethidium Bromide(EtBr) which is highly toxic and used for staining dsDNA, ssDNA or
RNA in agarose gels. EcoDye™ Nucleic Acid Staining Solution forms a complex through combination with DNA and can be detected
by UV imaging or LED system.
Feature
• Alternatives to EtBr • Detection of dsDNA, ssDNA & RNA
• High sensitivity • Various stability tests passed
• Non-toxic, non-mutagenic and non-carcinogenic (Ames test, Micronucleus test & In vitro mammalian
cell chromosomal aberration test)
• Excitation 290 – 320 nm, Emission 525 – 530 nm
Comparison of activity on various detection methods
Gel - Doc UltraSlim Transilluminator BlueView
(Wavelength : 312nm, UV) (Wavelength : 302nm, UV) (Wavelength : 470nm, Visible light)
1.5% Agarose Gel Staining (Pre-casting Step)
DNA / RNA
Sample Loading
Mixing 1 X TAE Mixing EcoDye TM Agarose gel
Bu er 100 ㎖ + Heating 3~5 mins Nucleic Acid Staining
Agarose 1.5 g Solution 10 ㎕ solution casting
201
BIOFACT CATALOGUE
QC DATA
- Comparison of uorescence intensity between EcoDye™ Nucleic Acid Staining Solution, the other company's
EtBr alternative and EtBr
<Multiplex PCR>
EtBr EcoDye™ Company A
M 1 2 3 4 5 6 M 1 2 3 4 5 6 M 1 2 3 4 5 6
M : Rice marker 1 : PCR product 12 ㎕ 2 : PCR product 10 ㎕ 3 : PCR product 8 ㎕ 4 : PCR product 6 ㎕ 5 : PCR product 4 ㎕ 6 : PCR product 2 ㎕
Multiplex(Rice) PCR product 1.0X TAE buffer 3% Agarose gel (10 ㎕ mix /100 ㎖ agarose gel solution)
- Activity test of Ecodye in agarose gel by the passage of observation time
On the day After 1 week After 2 weeks After 3 weeks
1 1 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5
1 : 1kb DNA Ladder 5㎕ 2 : 1kb DNA Ladder 4㎕ 3 : 1kb DNA Ladder 3㎕ 4 : 1kb DNA Ladder 2㎕ 5 : 1kb DNA Ladder 1㎕
1% Agarose gel
- Comparison of band migration shifts of EtBr alternative between EcoDye™ Nucleic Acid Staining Solution and other
EtBr alternatives
BIOFACT Company A Company B Company C
M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M
M : 1 kb DNA Ladder 1-6: Template volume
1 : Human gDNA 100 ng 2 : Human gDNA 50 ng 3 : Human gDNA 25 ng 4 : Human gDNA 12.5 ng 5 : Human gDNA 6 ng 6 : Human gDNA 3 ng
Multiplex PCR using zincfinger pimer 3% Agarose gel, 7 ㎕ loading
202 BIOFACT CATALOGUE
Ligase & Electrophoresis Reagent
Product Information
EcoDye™ Nucleic Acid Staining Solution [ES301-1000]
▶Contents
Nucleic Acid Staining Solution [500 ㎕ x 2 ea]
Reference
1. Lee H, Park JR, Kim EJ, Kim WJ, Hong SH, Park SM, Yang SR. Cigarette smoke-mediated oxidative stress induces apoptosis via the MAPKs/STAT1 pathway in mouse lung
broblasts. Toxicol Lett. 240:140-8, 2016
2. Lin T, Oqani RK, Lee JE, Shin HY, Jin DI. Coculture with good-quality COCs enhances the maturation and development rates of poor-quality COCs. Theriogenology.
85:396-407. 2016.
Maker Cat.No. Product Size
BIOFACT ES301-1000 Ecodye™ Nucleic Acid Staining Solution 500 ㎕ x 2 ea
203
BIOFACT CATALOGUE
BioFACT™
DNA Ladder & 6X Loading Dye
Sizing of a Wide Range of Double-Stranded DNA
BioFACT™ DNA Ladders is produced with mixed plasmids which are linearized using mass production, high puri cation step and
restriction enzyme treatment. These products can be available directly without additional manipulation and long-term storage is
available by mixed DNA preserved agent. BioFACT™ DNA Ladders is composed of two types according to the band sizes (100bp
plus/1kb plus).
Feature
• High speci c band • High-concentration DNA band
• Convenient use without additional step (100bp Plus : 100 ng/㎕, 1kb Plus : 150 ng/㎕)
• Mixed loading dye : Bromophenol blue & Xylene cyanol FF • 6X loading dye included
Application
• 1 kb Plus : Broad range size (0.1 ~ 10 kb) • 100 bp Plus : Small size (0.1 ~ 3 kb)
DNA Ladder (1kb / 100 bp)
204 BIOFACT CATALOGUE
Ligase & Electrophoresis Reagent
QC DATA
- Comparison of band intensity and number of bands between BioFACT™ 1 kb Plus DNA Ladder and
other companies' DNA ladders
1 2 3 4
1 2 3 4
1 2 3 4
1 : BioFACT™ 1kb Plus DNA Ladder
2 : Company A
1 : BioFACT TM 1kb DNA Ladder
3 : Company B
1 : BioFACT TM 1kb DNA Ladder
2 : B사 1kb DNA Ladder
2 : B사 1kb DNA Ladder
3 : E사 1kb DNA Ladder
4 : Company C
1 : BioFACT TM 1kb DNA Ladder
3 : E사 1kb DNA Ladder
4 : F사 1kb DNA Ladder
2 : B사 1kb DNA Ladder
4 : F사 1kb DNA Ladder
3 : E사 1kb DNA Ladder
1.5% Agarose gel
1.5% Agarose gel
4 : F사 1kb DNA Ladder
5 ㎕ loading
1.5% Agarose gel
5 ㎕ loading
5 ㎕ loading
40 min running (200 mA)
1.5% Agarose gel
40 min running (200 mA)
40 min running (200 mA)
5 ㎕ loading
40 min running (200 mA)
* Band intensity : BIOFACT = Company A > Company C > Company B
* Band Intensity : BIOFACT = B사 > F > E
* Number of bands : Company B > BIOFACT > Company C > Company A
* Band Intensity : BIOFACT = B사 > F > E
* Band Number : E 사 > BIOFACT > F사 > B사
* Band Number : E 사 > BIOFACT > F사 > B사
* Band Intensity : BIOFACT = B사 > F > E
* Band Number : E 사 > BIOFACT > F사 > B사
- Comparison of band intensity and number of bands between BioFACT™ 100 bp Plus DNA Ladder and
other companies' DNA ladders
1 2 3
1 2 3
1 2 3
1 : BioFACT™ 100bp Plus DNA Ladder
1 : BioFACT TM 100bp DNA Ladder
2 : Company A
1 : BioFACT TM 100bp DNA Ladder
2 : E사 100bb DNA Ladder
3 : Company B
2 : E사 100bb DNA Ladder
3 : I사 100bp DNA Ladder
1 : BioFACT TM 100bp DNA Ladder
3 : I사 100bp DNA Ladder
2 : E사 100bb DNA Ladder
1.5% Agarose gel
3 : I사 100bp DNA Ladder
1.5% Agarose gel
1.5% Agarose gel
5 ㎕ loading
5 ㎕ loading
5 ㎕ loading
40 min running (200 mA)
1.5% Agarose gel
40 min running (200 mA)
40 min running (200 mA)
5 ㎕ loading
40 min running (200 mA)
* Band Intensity : BIOFACT = E사 > I사
* Band intensity : BIOFACT = Company A > Company B
* Band Intensity : BIOFACT = E사 > I사
* Band Number : BIOFACT > E사 > I사
* Number of bands : BIOFACT > Company A > Company B
* Band Number : BIOFACT > E사 > I사
* Band Intensity : BIOFACT = E사 > I사
* Band Number : BIOFACT > E사 > I사
- Comparison of electrophoresis data between BioFACT™ DNA ladder series and other companies’ DNA ladders
1 2 3 4 5 6 7
1 2 3 4 5 6 7
1 2 3 4 5 6 7
1 : Company E 1kb DNA Ladder
1 : E사 1kb DNA Ladder
2 : Company B 1kb DNA Ladder
1 : E사 1kb DNA Ladder
2 : B사 1kb DNA Ladder
3 : Company C 1kb DNA Ladder
2 : B사 1kb DNA Ladder
3 : I사 1kb DNA Ladder
1 : E사 1kb DNA Ladder
4 : Company D 1
3 : I사 1kb DNA Ladderkb DNA Ladder
4 : G사 1kb DNA Ladder
2 : B사 1kb DNA Ladder
4 : G사 1kb DNA Ladder
3 : I사 1kb DNA Ladder
5 : BioFACT TM 1kb Plus
5 : BioFACT™ 1kb Plus DNA Ladder
5 : BioFACT TM 1kb Plus
6 : BioFACT TM 100bp Plus
4 : G사 1kb DNA Ladder
6 : BioFACT™ 100bp Plus DNA Ladder
6 : BioFACT TM 100bp Plus
7 : G사 100bp DNA Ladder
5 : BioFACT TM 1kb Plus
7 : Company D 100bp DNA Ladder
7 : G사 100bp DNA Ladder
6 : BioFACT TM 100bp Plus
1.5% Agarose gel
7 : G사 100bp DNA Ladder
1.5% Agarose gel
5 ㎕ loading
1.5% Agarose gel
5 ㎕ loading
1.5% Agarose gel
40 min running (200 mA)
5 ㎕ loading
40 min running (200 mA)
5 ㎕ loading
40 min running (200 mA)
40 min running (200 mA)
205
BIOFACT CATALOGUE
Product Information
BioFACT™ 1 kb Plus DNA Ladder [SM354-500]
▶Contents
1 kb Plus DNA Ladder [100 ㎕ x 5 ea]
6X Loading Dye [1 ㎖]
BioFACT™ 100 bp Plus DNA Ladder [SM342-500]
▶Contents
100 bp Plus DNA Ladder [100 ㎕ x 5 ea]
6X Loading Dye [1 ㎖]
Reference
1. EASWARAN, Maheswaran, et al. Functional characterization of a novel lytic phage EcSw isolated from Sus scrofa domesticus and its potential for phage therapy.
Molecular and cellular probes, 2015, 29.3: 151-157.
2. Song MA, Park JS, Kim KD, Jeun YC. E ect of X-irradiation on Citrus Canker Pathogen Xanthomonas citri subsp. citri of Satsuma Mandarin Fruits. Plant Pathol J.
31:343-9. 2015.
Maker Cat.No. Product Size
SM354-500 BioFACT™ 1 Kb Plus DNA Ladder 0.1㎖ × 5ea
BIOFACT SM342-500 BioFACT™ 100bp Plus DNA Ladder 0.1㎖ × 5ea
LD362-20h BioFACT™ 6X Loading dye (Bromophenol blue) 1㎖ × 2ea
206 BIOFACT CATALOGUE
Ligase & Electrophoresis Reagent
BioFACT™
Triple Color Protein Marker
Easy Identi cation by Three Di erent Colors
BioFACT™ Triple Color Protein Marker is designed for easy identi cation by three di erent colors to check the sizes of proteins during protein elec-
trophoresis step. It is composed of 10-23 di erent types of proteins and All prestained protein ladders are compatible for use in western blotting.
BioFACT™ Triple Color Protein Marker is a product made by purifying native protein, and there may be slight variations in protein size for each
production batch.
Feature
• Easy identi cation by three di erent colors • Directly loading on gels available
• Optimal design for western blot and stripping • SDS-PAGE buffer system (Glycine, MOPS and MES Buffer) available
10-180 kDa [SM306-500] Membrane Transfer
<Before>
10 ~ 180 kDa 10 ~ 245 kDa 10 ~ 180 kDa 10 ~ 245 kDa
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
12% SDS-PAGE 15% SDS-PAGE
<After>
10 ~ 180 kDa 10 ~ 245 kDa 10 ~ 180 kDa 10 ~ 245 kDa
10-245 kDa [SM307-500] 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
12% SDS-PAGE 15% SDS-PAGE
Nylone membrane transfer (300 mA, 1.5 hr), Tris-Glycine buffer system
1 : 7.5 ㎕ loading 2 : 5.0 ㎕ loading 3 : 2.5 ㎕ loading 4 : 1.25 ㎕ loading
F 사 BIOFACT F 사 E 사 BIOFACT
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
BIOFACT CATALOGUE 207
10% SDS-PAGE 10% SDS-PAGE
10 ~ 180 kDa
10 ~ 245 kDa
3
1
4
2
4
1
2
3
2
3
2
3
15% SDS-PAGE
12% SDS-PAGE
10 ~ 245 kDa
10 ~ 245 kDa
10 ~ 180 kDa
10 ~ 180 kDa
2
1
3
4
2
3
2
3
3
2
1
1
4
15% SDS-PAGE
12% SDS-PAGE 1 10 ~ 180 kDa 4 4 1 1 10 ~ 245 kDa 4 4
QC DATA
- Comparison of band intensity between BioFACT™ Triple Color Protein Marker and other companies' protein markers
<10 ~ 180 kDa> <10 ~ 245 kDa>
BIOFACT
E 사
F 사
BIOFACT
Company A BIOFACT Company A Company E BIOFACT
F 사
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
10% SDS-PAGE 10% SDS-PAGE
1 : 7.5 ㎕ loading 2 : 5.0 ㎕ loading 3 : 2.5 ㎕ loading 4 : 1.25 ㎕ loading
- Comparison of band intensity between BioFACT™ Triple Color Protein Marker and other companies' protein markers
A B C D E F G H I J
loading volume
A: Company A
B: Company B-1
C: Company B-2
D : BIOFACT (SM306-500)
E : BIOFACT (SM307-500)
F: Company C-1
G: Company C-2
H: Company D-1
I: Company E
J: Company D-2
Precast gel NP0615
Bis-Tris 10%, MES
110V, 74 min
SimpleBlue stained
- Band view before & after Coomassie blue staining treatment
A B C D E F G H I A B C D E F G H I
A: Company A
B: BIOFACT (SM306-500)
C: BIOFACT (SM306-500)
D: BIOFACT (SM306-500)
E: BIOFACT (SM307-500)
F : Company B
G : Company C
H : RNase A (13.7 kDa)
I : Lysozyme (14.3 kDa)
15% SDS-PAGE gel
Tris-Glycine
< Before coomassie brilliant blue staining > < After coomassie brilliant blue staining >
* The sizes of protein marker may be slightly di erent depending on each product.
* Protein sizes may appear slightly di erent depending on the type of prestained / unstained protein marker.
* Protein sizes may appear slightly di erent depending on the type of PAGE running bu ers.
208 BIOFACT CATALOGUE
Ligase & Electrophoresis Reagent
Product Information
BioFACT™ Triple Color Protein Marker (10 ~ 180 kDa) [SM306-500]
▶Contents
Triple Color Protein Marker (10~180 kDa)
[250 ㎕ x 2 ea]
BioFACT™ Triple Color Protein Marker (10 ~ 245 kDa) [SM307-500]
▶Contents
Triple Color Protein Marker (10~245 kDa)
[250 ㎕ x 2 ea]
Reference
YOO, Jae-Myung, et al. Inhibitory e ect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL-2H3 cells. International journal of mo-
lecular medicine, 2016, 37.2: 501-508.
Maker Cat.No. Product Size
SM306-500 BioFACT™ Triple Color Protein Marker (10 ~ 180 kDa) 250 ㎕ x 2 ea
BIOFACT
SM307-500 BioFACT™ Triple Color Protein Marker (10 ~ 245 kDa) 250 ㎕ x 2 ea
* It can be used more than 100 tests when loading 3-5 μl per each test.
209
BIOFACT CATALOGUE
HiGene™
Pre-made buffer
Convenient Solution for Molecular Biology and Biochemical Applications
HiGene™ Pre-made bu er is designed for convenient use and it is suitable for molecular biology and biochemical applications
such as nucleic acid gel electrophoresis, blotting, and DNA extraction.
Product Information
HiGene™ Pre-made bu er
Bu er composition Bu er size Storage temp. How to use
HiGene™
50X TAE bu er 2M Tris-Acetate, 50 mM EDTA 1 L RT Dilute 50X bu er to 1X or 0.5X.
[PB101-10h]
HiGene™ 445mM Tris, 445mM Boric Acid, Put it in a gel tank and use it or use
5X TBE bu er 10mM EDTA pH8.0 1 L RT it when making gel.
[PB102-10h]
HiGene™
RNase Free water (DEPC) Diethyl pyrocarbonate (DEPC) 0.1% 1 ㎖ x 81 ea RT RT-PCR & RNA sample preparation, etc.
[PB141-081,PB141-B500] / 500 ㎖
HiGene™ 0.5M EDTA 0.5M EDTA 500 ㎖ RT Dilute it at the appropriate
[PB131-500] concentration and use it.
HiGene™
10X SDS PAGE running 250mM Tris-HCl (pH8.3), Dilute 10X buffer to 1X and
bu er 1.92M glycine, 1% SDS 1 L RT use it for SDS gel loading.
[PB151-10h]
HiGene™
Coomassie blue staining 50% Methanol, 0.05% Coomassie
solution brilliant blue R-250, 10% Acetic acid 1 L RT Use it for SDS-PAGE gel stain.
[PB152-10h]
HiGene™
Coomassie blue destain- 45% Methanol, 10% Acetic acid 1 L RT Use it for SDS-PAGE gel stain.
ing solution
[PB153-10h]
210 BIOFACT CATALOGUE
Ligase & Electrophoresis Reagent
211
BIOFACT CATALOGUE
Lab
Supplies
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
Plasticware
96 Well Cell Culture Plate 214
Sealing Material & PCR Plate 215
PCR Tube (Conventional) 216
PCR Tube (Real-time) 217
PCR Tube Rack 218
Micro Tube 219
Other supplies
Al Cooling Block 224
96 Well Cell Culture Plate
[BIOFACT]
96 Well Cell Culture Plate of Various Scales
96 Well Collection Plate (U-bottom)
0.2 ㎖ Collection Plate (U-bottom) (PU-961h)
EO gas sterilization (Individual packaging)
Polystyrene (PS) material
96 Well Deep Well Plate (U-bottom)
• Convenient and stable sample storage for cell and tissue culture applications
2.2㎖ Deep Well Plate (DP-221h) 1.0㎖ Deep Well Plate (DP-1020)
Maker Cat.No. Product Size
PU-961h Hisol™ 96 Well Collection Plate -0.2 ㎖ ( U-bottom) 100 ea / box
BIOFACT DP-1020 Hisol™ 96 Square Deep Well Plate -1.0 ㎖ 10 ea x 2 pack / box
DP-221h Hisol™ 96 Square Deep Well Plate -2.2 ㎖ (pro le concave) 100 ea / box
214 BIOFACT CATALOGUE
Lab Supplies Plasticware
Sealing Material & PCR Plate
Highly Durable Sealing Material & PCR Plate
BioFACT™ PCR Plate Adhesive Seal, BioFACT™ Storage Sealing lm for ELISA/ Hisol™ 96 Well Silicon cap mat
[BFPCR-100] EIA, [EP301-100] [For PCR plate], [SR-9610]
• Excellent adhesion • High storage durability (-40℃ ~ 80℃) • For PCR 96 Well Plate
• High storage durability (-40℃ ~ 110℃) • Low evaporation rate (Less than 5%) • Autoclavable
• Optimized for storage and transportation • Elisa/ Eia/ Storage use • Excellent resistance to high temperatures
of PCR plates • Dimension : 142 x 78 x 1 (WxDxH, mm)
• Dimension : 137 x 80 x 1 (WxDxH, mm)
96 Well Silicon Cap Mat Hisol™ 0.2㎖ Standard Pro le PCR Hisol™ 0.2㎖ Standard Pro le qPCR
[For Deep-Well Plate], [DM-9610] 96 Well Plate (non-skirt) [PW681-050] 96 Well Plate (semi-skirted) [PW683-050]
• For 96 Deep Well Plate • Standard well & Clear type • Standard well & Clear type
• Autoclavable • Non-skirted Ultra thin wall design • Semi-skirted
• Sample protection from evaporation • Low evaporation rate (Less than 5%) • Ultra thin wall design
• High compatibility with various • Low evaporation rate (Less than 5%)
major instrument • High compatibility with various
• Dimension : 125 x 85 x 20 (WxDxH, mm) major instrument
• Dimension : 125 x 85 x 20 (WxDxH, mm)
Maker Cat.No. Product Size
BFPCR-100 BioFACT™ PCR Plate Adhesive Seal 100 sheet / box
EP301-100 BioFACT™ Storage Sealing Film for ELISA / EIA 10 sheet x 10 pack
SR-9610 Hisol™ 96 Well Silicon Cap Mat (PCR plate) 10 ea / box
BIOFACT
DM-9610 Hisol™ 96 Well Silicon Cap Mat (Deep Well Plate) 10 ea / box
PW681-050 BioFACT™ 0.2 ㎖ Standard Pro le PCR 96 Well Plate (non-skirt) 50ea/box
PW683-050 BioFACT™ 0.2 ㎖ Standard Pro le qPCR 96 Well Plate (semi-skirted) 50ea/box
215
BIOFACT CATALOGUE
0.2 ㎖ Conventional PCR
Tube Series [BIOFACT]
Outstanding Reproducibility and High Quality, HiGene™ PCR Tube Series
Application
• Non-pyrogenic • Autoclavable • Two different cap types; Flat & Domed cap
• RNase / DNase Free • Various tube colors
Cap type Flat Cap Type
Domed type
* Flat Type
Flat type * Domed Type * Natural [SPW01-T120] * Green [SPW04-T120] * Red [SPW02-T120]
[PW251-30h] [PW231-120] [PW211-120]
Flat Cap Type
* Natural [TS-08-CC]
Attached cap type (SPW11-T120)
* Yellow [SPW05-T120] * Blue [SPW03-T120] * Violet [SPW07-T120]
[SPW11-T120] [PT111-120] [PT174-120]
Separated cap type (PW231-120)
Domed Cap Type
Domed Cap Type
[PW221-120] [SPW01-T120]
separated cap type (PW221-120)
[PT101-120] [PT164-120]
Maker Cat.no Product Size
PT101-120 HiGene™ 0.2 ㎖ 8-Strip Tubes - individually attached domed cap (natural) 12 ea x 10 pack
PT111-120 HiGene™ 0.2 ㎖ 8-Strip Tubes - individually attached at cap (natural) 12 ea x 10 pack
PT164-120 HiGene™ 0.2 ㎖ 8-Strip Tubes - individually attached domed cap (green, sterilized) 12 ea x 10 pack
PT174-120 HiGene™ 0.2 ㎖ 8-Strip Tubes - individually attached at cap (green, sterilized) 12 ea x 10 pack
PW211-120 Hisol™ 0.2 ㎖ 8-Strip Tubes - individually attached at cap (natural) 12 ea x 10 pack
BIOFACT
PW221-120 Hisol™ 0.2 ㎖ 8-Strip Tubes - separate domed cap 12 ea x 10 pack
PW231-120 Hisol™ 0.2 ㎖ 8-Strip Tubes - separate at cap 12 ea x 10 pack
PW251-30h Hisol™ 0.2 ㎖ PCR Tube - individually attached at cap (single tube) 500 tube × 6 pack
SPW01-T120 Hisol™ 0.2 ㎖ 8-Strip Tubes - individually attached domed cap (natural) 12 ea x 10 pack
SPW11-T120 Hisol™ 0.2 ㎖ 8-Strip Tubes - individually attached at cap (natural) 12 ea x 10 pack
216 BIOFACT CATALOGUE
Lab Supplies Plasticware
0.1 ㎖ 8-Strip Real-Time PCR
Tube [BIOFACT]
Exceptional 8-strip tube for Real-Time PCR Applications
Feature 0.1㎖ Low Pro le Comparison data for variation test between wells
일반적인 0.2㎖ tube의 경우와 달리, tube 내 빈 공간(Dead
0.1㎖ Low Pro le Delta Rn vs Cycle
Delta Rn vs Cycle
Space)을 최소화 시키므로 더욱 향상된 열 전도율과 qPCR
1.0e + 001
Its low-pro le design reduces dead space and increases PCR 1.0e + 001
e ciency through enhanced heat transfer. BIOFACT
반응이 가능합니다.
1.0e + 000
1.0e + 000
Low Pro le High Pro le
1.0e - 001
1.0e - 001
1.0e - 002
1.0e - 002
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35
Delta Rn vs Cycle
Delta Rn vs Cycle
1.0e + 001
1.0e + 001
• 0.1㎖ low Pro le
• Thin-wall design Company A
White Body
1.0e + 000
• Enhanced heat transfer 1.0e + 000
• Autoclavable
Well간의 간섭현상을 막아주고 uorescence re ection을
• Consistency between wells 1.0e - 001
1.0e - 001
강화시키는 불투명한 White body로 인해, 더욱 정확한 측정
• Best compatibility with various qPCR instrument
가능(투명한 tube도 많이 사용되고 있으나, 정확한 Real-time
1.0e - 002
1.0e - 002
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35
PCR반응을 위해서는 전용제품 사용을 권장 드립니다)
Frosted Tube(F) Type [PW261-120] White Tube(W) Type [PW262-120] Clear Tube(C) Type [PW263-120]
Frosted Tube(F) Type [PT301-120] White Tube(W) Type [PT302-120] Clear Tube(C) Type [PT303-120]
White Clear Clear
Optically Clear & Flat Cap
Maker Cat.no Product Size
실시간(Real-time)으로 정확한 형광 값을 측정하기 위해 광
PW261-120
Hisol™ 0.1 ㎖ qPCR 8-Strip Frosted Tubes - separate optical at cap
학적으로 매우 투명한 상태의 Cap을 사용해야 합니다. 12 ea × 10 pack
PW262-120 Hisol™ 0.1 ㎖ qPCR 8-Strip White Tubes - separate optical at cap 12 ea × 10 pack
PW263-120 Optically clear Cap 12 ea × 10 pack
Hisol™ 0.1 ㎖ qPCR 8-Strip Clear Tubes - separate optical at cap
BIOFACT
PT301-120 HiGene™ 0.1 ㎖ qPCR 8-strip Frosted Tubes - separate optical at cap 12 ea × 10 pack
PT302-120 HiGene™ 0.1 ㎖ qPCR 8-strip White Tubes - separate optical at cap 12 ea × 10 pack
PT303-120 HiGene™ 0.1 ㎖ qPCR 8-strip Clear Tubes - separate optical at cap 12 ea × 10 pack
217
BIOFACT CATALOGUE
White Body
0.2 ㎖ PCR Tube Rack
[BIOFACT]
Perfect convenience for pre or post PCR sampling
Description
• Heavy duty polypropylene • 96 well PCR plate applicable
• Strong durability • Safe sample storage through lid
• Size : 125(L) x 85(W) x 15(H) mm • Easy stacking by molding treatment
• Color : Amber, Sky blue • Imprinted alpha/numeric grid identification
• 96 hole ( 8 x 12 array) for 0.2 ㎖ PCR tube • Autoclavable
Easy to distinguish the direction of the rack through edge
cutting design Lid can be opened, closed or xed depending on how to grab it.
0.2 ㎖ PCR Tube Rack [PR-0200] 0.2 ㎖ PCR Tube Rack [PR-0200SB]
구성품목 (Contents)
▶Contents
구성품목 (Contents) 구성품목 (Contents)
구성품목 (Contents)
▶Contents
mber)
A
ack (
PCR Tube Rack (Sky Blue)
T
ube R
y Blue)
PCR Tube Rack (Amber)
T
ack (Sk
ube R
PCR PCR Tube Rack (Amber) PCR PCR Tube Rack (Sky Blue)
Cover (Clear) Cover (Clear)
Maker Cat.No. Product Size
PR-0200 Hisol™ 0.2 ㎖ PCR Tube Rack (Amber) EA
BIOFACT
PR-0200SB Hisol™ 0.2 ㎖ PCR Tube Rack (SkyBlue) EA
218 BIOFACT CATALOGUE
Lab Supplies Plasticware
1.5 ㎖ Micro Tube
[BIOFACT]
Universal & High-Quality 1.5㎖ Micro Centrifuge Tube
Description
• Autoclavable • Graduated for easy volume identification
• Smooth open & tight closure • Moulded from virgin polypropylene
• Zip lock bag packaging for convenient storage
1.5㎖ Micro Tube [MT-1500]
Tip.
How to prevent the cap of the tube from breaking on centrifuge
Please insert 1.5㎖ tubes into centrifuge with their caps
crossed to prevent them from breaking.
Maker Cat.No. Product Size
BIOFACT MT-1500 Hisol™ 1.5㎖ Micro Tube 500 ea x 10 pack
219
BIOFACT CATALOGUE
1.5 ㎖ Micro Tube Blue
[BIOFACT]
Ready to use, Sterilized 1.5 ml Micro Tube
Description
• Superior E-beam treatment • No autoclaving required
• Smooth opening & tight closing • Graduated for easy volume identification
• Great quality & a ordable price • Max. centrifugal force: 16000XG
* E-beam (electron beam) Treatment
Electron beam is a form of ionizing energy using electrons that is generally characterized by its low penetration and high dosage rates.
E-beam sterilization guarantees greater quality and more e ective sterilization than gamma radiation sterilization.
1.5 ㎖ Micro Tube, Blue [EMT-1530]
1.5 ㎖ Micro Tube, Blue [EMT-1530pk]
Maker Cat.No. Product Size
EMT-1530 Hisol™ 1.5 ㎖ Micro tube, Blue (Electron Beam Irradiated) 300 ea / bottle
BIOFACT
EMT-1530pk Hisol™ 1.5 ㎖ Micro tube, Blue (Electron Beam Irradiated) 300 ea x 15 bottle
220 BIOFACT CATALOGUE
Lab Supplies Plasticware
221
BIOFACT CATALOGUE
Lab
Supplies
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
Plasticware
96 Well Cell Culture Plate 214
Sealing Material & PCR Plate 215
PCR Tube (Conventional) 216
PCR Tube (Real-time) 217
PCR Tube Rack 218
Micro Tube 219
Other supplies
Al Cooling Block 224
Al Cooling Block [BIOFACT]
Stable Temperature-Retention for Reagents
BioFACT Al Cooling Block의 시간별 온도변화
TM
AI Cooling Block is designed to store samples safely on the lab bench without sample degradation due to temperature increase or
(상온 보관 후 ice 위에 올려 놓고 사용 시)
uctuation. AI Cooling Block is made of 99% aluminum which has highest thermal conduction rate and it maintains temperature
at approximately 0° C within 10 minutes when placing on the ice.
Through simply placing the AI Cooling Block in a freezer for two hours, it is also ready for use and it can keep samples cool and
safe at the bench without ice buckets.
- Minimization of degradation due to temperature - Excellent corrosion resistance & low temperature strength
- Usage with ice bucket / without ice bucket
상온 보관 중인 Al cooling block을 ice bucket위에 올려 놓고,
시간별 온도 변화를 측정
상온에 보관했던 Al cooling block의 초기 온도는 18℃ (ice 2℃)에서
How to use 약 1분 뒤에 10℃가 되었고 그 후에는 거의 비슷한 온도로 유지
TM
BioFACT Al Cooling Block의 시간별 온도변화 BioFACT Al Cooling Block의 시간별 온도변화
TM
(상온 보관 후 ice 위에 올려 놓고 사용 시) (-20℃ 보관 후 ice 위에 올려 놓고 사용)
• With ice (bucket) : • With-out ice:
Place AI Cooling Block on the Place AI Cooling Block in the
ice or iced pack a minute ago below -20 ℃ freezer for 2 hours
from starting the experiment. before use.
상온 보관 중인 Al cooling block을 ice bucket위에 올려 놓고,
시간별 온도 변화를 측정 -20℃ 보관 해 미리 온도를 낮춰 놓은 Al cooling block을 ice
상온에 보관했던 Al cooling block의 초기 온도는 18℃ (ice 2℃)에서
약 1분 뒤에 10℃가 되었고 그 후에는 거의 비슷한 온도로 유지 bucket 위에 올려 놓고 시간별 온도 변화를 측정
Comparison of temperature changes according to the passage of time
TM
TM
BioFACT Al Cooling Block의 시간별 온도변화 BioFACT Al Cooling Block의 3) In case of usage without ice after storage
2) In case of usage with ice after storage 시간별 온도변화 BioFACT Al Cooling Block의 시간별 온도변화
TM
1) In case of usage with ice
(실험 테이블에 놓고 사용 시)
(상온 보관 후 ice 위에 올려 놓고 사용 시) at -20 ℃ freezer at -20 ℃ freezer, without ice or an ice bucket
(-20℃ 보관 후 ice 위에 올려 놓고 사용)
상온 보관 중인 Al cooling block을 ice bucket위에 올려 놓고,
상기 실험 데이터는 실내 환경 조건에 따라 차이가 있을 수 있습니다.
Before placing AI Cooling Block on the ice, initial -20℃ 보관 해 미리 온도를 낮춰 놓은 Al cooling block을 ice Experimental data may di er depending on the
Temperature changes was measured over time
시간별 온도 변화를 측정
temperature of AI Cooling Block was 18 ℃ (ice 2 bucket 위에 올려 놓고 시간별 온도 변화를 측정 indoor environmental conditions.
after placing AI Cooling Block stored at -20℃
상온에 보관했던 Al cooling block의 초기 온도는 18℃ (ice 2℃)에서
℃). A minute later after AI Cooling Block placing on freezer in advance on an ice bucket.
약 1분 뒤에 10℃가 되었고 그 후에는 거의 비슷한 온도로 유지
the ice, the temperature of it turned out to 10℃. Tip.
TM
After that, similar temperature was maintained in BioFACT Al Cooling Block의 시간별 온도변화
AI Cooling Block. TM ① -20℃ 이하의 냉동고에 보관 해야지만 Al Cooling block
BioFACT Al Cooling Block의 시간별 온도변화 (실험 테이블에 놓고 사용 시)
(-20℃ 보관 후 ice 위에 올려 놓고 사용) 의 전체 온도가 영하로 내려갑니다.
② 미리 얼려놓은 Al cooling block의 온도를 30분 이상
영하로 유지하기 위해서는 반드시 Ice bucket을 사용
Tip. 하셔야 합니다.
① The temperature of AI Cooling Block can be down to minus degrees in case of storage at below -20 ℃ freezer. ③ 액체 질소 사용 시 기화되면서 온도가 내려가기 때문에
ice만큼 온도가 빨리 내려가지는 않습니다.
② Please use ice (bucket) with AI Cooling Block in order to keep minus degrees of the Al Cooling Block for more than 30 minutes which was kept at below -20 ℃ freezer.
③ The temperature of AI Cooling Block doesn’t go down as fast as ice due to vaporization when using liquid nitrogen.
상기 실험 데이터는 실내 환경 조건에 따라 차이가 있을 수 있습니다.
-20℃ 보관 해 미리 온도를 낮춰 놓은 Al cooling block을 ice
bucket 위에 올려 놓고 시간별 온도 변화를 측정
Tip.
① -20℃ 이하의 냉동고에 보관 해야지만 Al Cooling block
TM
BioFACT Al Cooling Block의 시간별 온도변화 의 전체 온도가 영하로 내려갑니다.
224 BIOFACT CATALOGUE ② 미리 얼려놓은 Al cooling block의 온도를 30분 이상
(실험 테이블에 놓고 사용 시)
영하로 유지하기 위해서는 반드시 Ice bucket을 사용
하셔야 합니다.
③ 액체 질소 사용 시 기화되면서 온도가 내려가기 때문에
ice만큼 온도가 빨리 내려가지는 않습니다.
상기 실험 데이터는 실내 환경 조건에 따라 차이가 있을 수 있습니다.
Tip.
① -20℃ 이하의 냉동고에 보관 해야지만 Al Cooling block
의 전체 온도가 영하로 내려갑니다.
② 미리 얼려놓은 Al cooling block의 온도를 30분 이상
영하로 유지하기 위해서는 반드시 Ice bucket을 사용
하셔야 합니다.
③ 액체 질소 사용 시 기화되면서 온도가 내려가기 때문에
ice만큼 온도가 빨리 내려가지는 않습니다.
Lab Supplies Other supplies
A Type B Type C Type D Type E Type
[SJ1-ACB01] [SJ1-ACB02] [SJ1-ACB03] [SJ1-ACB04] [SJ1-ACB05]
96 well x 0.2 ㎖ 12 hole x 1.5 ㎖ / 25 hole x 1.5 ㎖ / 40 hole x 1.5 ㎖ / 4 hole x 2.0 ㎖ /
96 well x 0.2 ㎖ 48 well x 0.2 ㎖ 96 well x 0.2 ㎖ 4 hole x 1.5 ㎖ /
16 well x 0.2 ㎖
Al Block for water bath
15 ㎖ Conical Tube Type
SJ1-ABW01L / SJ1-ABW01R SJ1-ABW02 [SJ1-ABW03]
48 Well x 0.2㎖, 4hole x 1.5 ㎖_Left side 20 hole x 15 ㎖ 11 hole x 15 ㎖
48 Well x 0.2㎖, 4hole x 1.5 ㎖_Right side
Al Block for Mini Heating Dry Bath
Reservoir Type Al Block for thermometer
SJ1-ABW04 [SJ1-ACBR01] (SJ1-ABT01)
12hole x 1.5 ㎖ Reservoir Type (V-shaped) 1hole in 5 faces
Maker Cat.No. Product Size
SJ1-ACB01 Al Cooling Block A type(96 Well x 0.2 ㎖) 12 x 8.2 x 1.5 ㎝
SJ1-ACB02 Al Cooling Block B type(12hole x 1.5 ㎖ / 96 Well x 0.2 ㎖) 12 x 12.2 x 3 ㎝
SJ1-ACB03 Al Cooling Block C type(25hole x 1.5 ㎖ / 48 Well x 0.2 ㎖) 12 x 10.5 x 3 ㎝
SJ1-ACB04 Al Cooling Block D type(40hole x 1.5 ㎖ / 96 Well x 0.2 ㎖) 12 x 16.5 x 3 ㎝
SJ1-ACB05 Al Cooling Block E type(4hole x 2.0 ㎖ / 4hole x 1.5 ㎖ / 16 Well x 0.2 ㎖) 8.5 x 8.5 x 3 ㎝
SJ1-ABW01L Al Block for water bath (48 Well x 0.2 ㎖ / 4hole x 1.5 ㎖_Left side ) 8 x 2.5 x 5 ㎝
BIOFACT
SJ1-ABW01R Al Block for water bath (48 Well x 0.2 ㎖ / 4hole x 1.5 ㎖_Right side ) 8 x 2.5 x 5 ㎝
SJ1-ABW02 Al Block for water bath (20hole x 1.5 ㎖ ) 8 x 10.3 x 5 ㎝
SJ1-ABW03 Al Block for water bath (11hole x 15 ㎖ ) 10.5 x 8 x 5 ㎝
SJ1-ABW04 Al Block for Mini Heating Dry Bath(12hole x 1.5 ㎖ ) 7 x 4.5 x 3 ㎝
SJ1-ACBR01 Al Cooling Block Reservoir Type (V-shaped) 12 x 4 x 3 ㎝
SJ1-ABT01 Al Block for thermometer ( each 1 hole in 5 faces ) 3 x 3 x 2.5 ㎝
225
BIOFACT CATALOGUE
Filtration
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
Mini Column
DNA / RNA Puri cation Mini Column 228
HiFilter (for removing debris) 228
Syringe Filter
CA (Cellulose Acetate) 231
MCE (Mixed Cellulose Ester) 231
PES (Polyethersulfone) 231
Nylon (Polyamide) 231
PVDF (Polyvinylidene Fluoride) 231
PTFE – W (Polytetra uoroethylene - Hydrophilic) 231
PTFE – O (Polytetra uoroethylene-Hydrophobic) 231
Membrane Filter
MCE (Mixed Cellulose Ester) 237
PES (Polyethersulfone) 237
Nylon (Polyamide) 237
PVDF (Polyvinylidene Fluoride) 237
PTFE (Polytetra uoroethylene - Hydrophobic) 237
HiGene™ DNA / RNA Purification
Mini Column [BIOFACT]
Perfect Column for DNA / RNA Puri cation
HiGene™ DNA / RNA Puri cation Mini Column is designed for isolation and puri cation of high purity plasmid, genomic DNA or
RNA for use in routine molecular biology. It is highly compatible with a wide range of bu ers manufactured from various brands
including BioFACT™ DNA/RNA Preparation Series. HiGene™ DNA / RNA Puri cation Mini Column is manufactured by polypro-
pylene and automatic robot system in clean room for its optimum performance and it is absolutely 100% DNase/RNase free.
Feature
• Spin column type • Elution volume : 30 – 400 ㎕
• Membrane : Glass micro ber • Maximum volume : 800 ㎕
• Column binding capacity : 20㎍ DNA (RNA) / Column • Electron beam sterilized
Application
• HiGene™ Genomic DNA Prep Kit (Column type) • HiGene™ Total RNA Prep Kit
• HiGene™ Plasmid Mini Prep Kit (Ver.2.0) • Prep kits from other brands (Column type)
• HiGene™ Gel & PCR Puri cation System / PCR Puri cation Kit
Open Column Type Cap Column Type HiFilter
[PM116-100] [GP119-100, GD156-100] [RP119-100]
Capsule Type [RP118-100]
Feature of HiFilter & Collection Tube
• Prevention of column-blockage through cell debris-removal
• Increase of final RNA isolation yield
• Non binding capacity of DNA/RNA
• It is recommended to use it before capsule column step
in case of total RNA isolation from plant samples
228 BIOFACT CATALOGUE
Filtration
HiGene™ DNA / RNA Purification Comparison of compatibility of HiGene™ DNA / RNA Puri cation Mini Column with other companies' prep
solution kits
Mini Column [BIOFACT] Extraction e ciency test on various sample types Puri cation e ciency test on various sizes of PCR product
Perfect Column for DNA / RNA Puri cation <Genomic DNA Preparation from Plant> <PCR Purification from 1kb PCR Product>
M 1 2 3 4 5 6 7 M M C 1 2 3 4 5 6 7 M
<1kb>
<Total RNA Preparation from Plant> <PCR Purification from 5kb PCR Product>
1 2 5 6 7 1 2 3 4 5 6 7
M M M C M
<5kb>
* Before DNase I treatment
<Plasmid mini prep from high copy plasmid> <Gel Extraction from 1kb PCR Product>
1 2 3 4 5 6 7 1 2 3 4 5 6 7
M M M C M
pUC 19 <1kb>
<Plasmid mini prep from low copy plasmid> <Gel Extraction from 5kb PCR Product>
M 1 2 3 4 5 6 7 M M C 1 2 3 4 5 6 7 M
pMW 118 <5kb>
M : DNA Ladder C : Control PCR Product (Before purification step)
1 : Company A Solution + Company A Column 2 : Company A Solution + BIOFACT Column 3 : Company B Solution + Company B Column
4 : Company B Solution + BIOFACT Column 5 : Company C Solution + Company C Column 6 : Company C Solution + BIOFACT Column
7 : BIOFACT Solution + BIOFACT Column
229
BIOFACT CATALOGUE
Plasmid Mini Prep [PM116-100] RNA Prep [RP118-100]
▶Contents ▶Contents
Open Column Capsule Column with
Collection Tube Collection Tube
Gel Extraction / PCR Puri cation [GP119-100] DNA/RNA Prep [RP119-100]
▶Contents ▶Contents
Cap Column HiFilter column
Collection Tube Collection Tube
Genomic DNA Prep [GD156-100]
▶Contents
Cap Column
Collection Tube
Maker Cat.No. Product Size
PM116-100 HiGene™ HiSpin Column with Collection tube (Plasmid Mini Prep) 50 ea x 2 pack
GP119-100 HiGene™ HiSpin Column with Collection tube (Gel Extraction , PCR Puri cation) 50 ea x 2 pack
BIOFACT GD156-100 HiGene™ HiSpin Column with Collection tube (Genomic DNA Prep) 50 ea x 2 pack
RP118-100 HiGene™ HiSpin Capsule Column with Collection tube (RNA Prep) 10 ea x 10 pack
RP119-100 HiGene™ HiFilter with Collection tube (DNA/RNA Prep) 50 ea x 2 pack
230 BIOFACT CATALOGUE
Filtration
Syringe Filter [BIOFACT]
Excellent in Rapid Filtration and Particle Removal
BioFACT™ Syringe Filter is most suitable for rapid puri cation and removal of particles from liquids and gases. Housing of all the
syringe lters is manufactured with polypropylene and it can o er broad chemical compatibility. All of our sterilization lters are
completely sterilized by electron-beam treatment which guarantees high quality and e ective sterilization. Furthermore 'female
luer lock typed inlet' and 'male luer slip typed outlet' of syringe lters enable user to load or unload it quickly and conveniently.
BioFACT™ Syringe Filter has a wide range of membrane types, pore sizes and sterile / non-sterile type to meet various application
needs.
Feature
• Rapid and robust puri cation / removal of particles * It is designed that solution is allowed to ow down in case
syringe lter is destroyed.
• Housing material: Polypropylene
• Completely sterilization by electron-beam treatment
• Broad chemical compatibility
• Easy mount and unmount
• Various membrane materials for di erent applications
• Sterile / Non-sterile type
BIOFACT Other syringe lters
Application
• Sterile ltration and particle removal ltration • Clarifying ltration, HPLC ltration applications
• Filtration of solutions containing protein, aqueous solutions, • Sterile venting of air or other gases during ltration
and mild organic solvents
• Filtration of various pharmaceutical, biological
and biotech solutions
Syringe lter & Packing type
- Non-sterilized type
(50ea, 100ea / bottle)
- Sterilized type (Individual packaging)
231
BIOFACT CATALOGUE
Feature of various membrane materials in syringe lter
CA PES MCE Nylon PVDF PTFE-W PTFE-O
Sterilized Sterilized Sterilized Non-sterilized Non-sterilized Non-sterilized Non-sterilized
Hydrophilic Hydrophilic & Hydrophobic Hydrophobic
Low protein binding High protein binding Low protein binding
Clarifying ltration,
Particle removal Filtration of culture Pre- ltration of Filtration of organic
Filtration of
ltration of biochem- medium, additive HPLC sample ltration aqueous solution Filtration of mild- mixed solvent used solvent / HPLC
oganic solvent
and particle removal
ical solution and bu er in HPLC sample
ltration
Sample volume
13 ㎜ for up to 10 ㎖ 25 ㎜ for up to 100 ㎖
Available Volume : 0.3 – 10 ㎖ Available Volume : 10 – 100 ㎖
＊Filtration capacity may be di erent on various solution type, character of solution or pH.
Feature of two pore sizes in syringe lter
Sterilization Particle Removal
0.2 ㎛ 0.45 ㎛
(Columns packed < 3 ㎛ particles) (Columns packed > 3 ㎛ particles)
Bacteria, UHPLC, etc. Particles, HPLC, etc.
Application notes of syringe lter
Type of Filtration Recommended Alternatives
HPLC · UHPLC · LC/MS · GC (Sample pretreatment) PTFE PVDF / Nylon
Undiluted Organic Solvents PTFE PVDF / Nylon
Protein Analysis · Samples with Biomolecules-Bu er PES CA / MCE / PVDF
Tissue Culture Media PES CA
High Particle-load Sample – Organic Solvents Hydrophobic PTFE-O PTFE / PVDF
High Particle-load Sample – Aqueous Solvents Nylon -
232 BIOFACT CATALOGUE
Filtration
Chemical Compatibility
MCE PES Nylon PVDF-W PTFE-O MCE PES Nylon PVDF-W PTFE-O
Acids Halogenated Hydro-
carbons
Acetic Acid ○ X X ○ ○ Carbon tetrachloride X △ △ ○ ○
Hydrochloric Acid ○ ○ X ○ ○ Chloroform ○ X △ ○ ○
Hydrochloric Acid (6N) X ○ X – ○ Ethylene Dichloride ○ X △ – –
Nitric Acid ○ – X ○ ○ Freon TF ○ ○ ○ ○ ○
Nitric Acid (6N) X – X ○ ○ Freon TMC ○ X △ – ○
Phosphoric Acid ○ – X – ○ Methylene Dichloride X X X – –
Sulfuric Acid ○ X X – X Perchloroethylene ○ △ – – –
Hydrofluoric Acid X – X X ○ Trichloroethylene ○ △ △ ○ ○
Bases Glycols
Ammonium Hydroxide (1N) ○ ○ ○ △ – Ethylene glycol – △ ○ ○ ○
Ammonium Hydroxide (3N) X ○ ○ – – Glycerol ○ △ ○ ○ ○
Potassium Hydroxide (3N) X ○ ○ ○ ○ Propylene Glycol – △ ○ – ○
Sodium Hydroxide (3N) X ○ ○ X ○ Ketones
Sodium Hydroxide (6N) X ○ ○ X ○ Acetone X X ○ X ○
Alcohols Cyclohexanone X X – X ○
Amyl Alcohol △ ○ ○ ○ ○ Methylethylketone – – △ X ○
Benzyl Alcohol (100%) X ○ ○ ○ ○ Methyl isobutylketone – X △ X ○
butyl alcohol △ ○ ○ ○ ○ Miscellaneous
Isopropyl alcohol △ ○ ○ ○ ○ Aniline – X △ – ○
Methanol △ ○ △ – ○ Di Methyl Formamide – X ○ X ○
Aromatic Hydrocarbons Formaldehyde (37%) – ○ ○ ○ ○
Benzene ○ – △ ○ ○ Gasoline ○ ○ △ ○ ○
Toluene ○ X X ○ ○ Hexane (dry) ○ △ – ○ ○
Xylene ○ X △ ○ ○ Petroleum – ○ – ○ ○
Ethers Phenol ○ X ○ ○ ○
Ethyl Ether ○ ○ X ○ ○ Pyridine X X △ X ○
Isopropyl Ether ○ – – – ○ Turpentine – ○ – – –
Dioxane X – ○ ○ ○ Water ○ ○ ○ ○ ○
Tetrahydrofuran X X X △ ○ Acetonitrile X ○ △ △ ○
Esters Sulfuric Acid Nickel Solution – – ○ – –
Amyl Acetate X – △ ○ ○
※ ○ : Resistance △ : Limited resistance x : No resistance - : No data available
Butyl Acetate X – △ – ○ • It is based on the material properties.
Cellosolve Acetate X ○ – – – • It is recommended to use it for reference only.
Ethyl Acetate X △ △ ○ ○
Methyl Acetate X X △ X ○
Isopropyl Acetate X ○ – ○ –
233
BIOFACT CATALOGUE
CA (Cellulose Acetate)
• Stability in pH : pH3-7 Dimension (mm) Pore size (㎛) Cat.No. Size
• Filtration of culture medium, additive and buffer 0.2 ㎛ BSS20-CA13
• Extremely low binding capacity for protein Φ 13 0.45 ㎛ BSS45-CA13 100 ea / box
• Filtration of solution and aqueous solution containing protein 0.2 ㎛ BSS20-CA25
• Sterilization : Yes (Individually packed) Φ 25 0.45 ㎛ BSS45-CA25 50 ea / box
MCE (Mixed Cellulose Ester)
• Stability in pH : pH 3.5 – 8
• Cellulose nitrate & cellulose acetate mixed material Dimension (mm) Pore size (㎛) Cat.No. Size
• Clarifying filtration and HPLC sample filtration Φ 13 0.2 ㎛ BSS20-MC13 100 ea / box
• Even and uniform pore size 0.45 ㎛ BSS45-MC13
• Sterilization by filtration for small amounts of liquids Φ 25 0.2 ㎛ BSS20-MC25 50 ea / box
• Superior strength / adsorptive power, 0.45 ㎛ BSS45-MC25
• Sterilization : Yes (Individually packed)
PES (Polyethersulfone)
• Stability in pH : pH 1 – 14
• Excellent heat / chemical resistance Dimension (mm) Pore size (㎛) Cat.No. Size
• Optimal for biological experiment 0.2 ㎛ BSS20-PE13
• Filtration of culture medium, additive and buffer Φ 13 BSS45-PE13 100 ea / box
• Extremely low binding capacity for protein 0.45 ㎛
• Filtration of solutions containing protein, aqueous solution Φ 25 0.2 ㎛ BSS20-PE25 50 ea / box
and mild organic solvent 0.45 ㎛ BSS45-PE25
• Sterilization : Yes (Individually packed)
Nylon (Polyamide)
• Stability in pH : pH 6 – 13
• Superior mechanical strength / adsorptive power Dimension (mm) Pore size (㎛) Cat.No. Size
• Superior hydrophilic feature Φ 13 0.2 ㎛ BS20-NY13 100 ea / box
• High binding capacity for protein 0.45 ㎛ BS45-NY13
• Efficient filtration of aqueous solution Φ 25 0.2 ㎛ BS20-NY25 50 ea / box
• Filtration of organic solvent and mid-polar liquid 0.45 ㎛ BS45-NY25
• Sterilization : No
PTFE-W (Polytetra uoroethylene-Hydrophilic)
• Hydrophilic PTFE filter Dimension (mm) Pore size (㎛) Cat.No. Size
• Excellent organic, acid and alkali solvent resistance 0.2 ㎛ BS20-PW13
• Low binding capacity for protein Φ 13 0.45 ㎛ BS45-PW13 100 ea / box
• Optimal for biological experiment 0.2 ㎛ BS20-PW25
• Pre-treatment filtration of mixed solvent used in HPLC Φ 25 0.45 ㎛ BS45-PW25 50 ea / box
• Sterilization : No
PVDF (Polyvinylidene Fluoride)
Dimension (mm) Pore size (㎛) Cat.No. Size
• Excellent thermal and chemical resistance 0.2 ㎛ BS20-PV13
• Low binding capacity for protein Φ 13 0.45 ㎛ BS45-PV13 100 ea / box
• General filtration available BS20-PV25
• Filtration of polar and non polar solution Φ 25 0.2 ㎛ 50 ea / box
• Sterilization : No 0.45 ㎛ BS45-PV25
PTFE-O (Polytetra uoroethylene-Hydrophobic)
Dimension (mm) Pore size (㎛) Cat.No. Size
• Hydrophobic filter with low friction 0.2 ㎛ BS20-PO13
• Inadhesive and detachable material Φ 13 0.45 ㎛ BS45-PO13 100 ea / box
• Easy removal of residues during filtration
• Filtration of HPLC sample and solvent Φ 25 0.2 ㎛ BS20-PO25 50 ea / box
• Organic solvent filtration available 0.45 ㎛ BS45-PO25
• Available to be used as an air vent filter * Hydrophobic PTFE has water repellency. In case of aqueous solution,
• Sterilization : No (Autoclavable) be sure to soak PTFE-O syringe lter in proper solvents
(Isopropyl alcohol or ethylene alcohol) before ltration.
234 BIOFACT CATALOGUE
Filtration
BioFACT™ Syringe Filter selection guide
Maker Cat.No. Millipore Millex® Whatman® Sartorius Minisart®
BSS20-CA13 - - -
BSS45-CA13 - 677 1-1304 [0.45㎛ / Ø13] -
CA BSS20-CA25 - - * 17597----------K, sterilized
* 17597----------Q
(Sterilized) [0.2㎛ / Ø28]
* 17598----------K, sterilized
* 17598----------Q
BSS45-CA25 - -
* 16569----------K, sterilized
[0.45㎛ / Ø28]
BSS20-MC13 - - -
BSS45-MC13 - - -
MCE
(Sterilized) SLGSV255F [0.22㎛ / Ø25]
BSS20-MC25 - -
SLGS033SS [0.22㎛ / Ø33], sterilized
BSS45-MC25 SLHAM33SS [0.45㎛ / Ø33], sterilized - -
6780-1302 [0.2㎛ / Ø13], sterilized 1776D-------ACK, sterilized
BSS20-PE13 SLGPX13NL [0.2㎛ / Ø13]
6782-1302 [0.2㎛ / Ø13] [0.2㎛ / Ø15]
6780-1304 [0.45㎛ / Ø13], sterilized
BSS45-PE13 SLHPX13NL [0.45㎛ / Ø13] -
6782-1304 [0.45㎛ / Ø13]
PES SLMP025SS [0.22㎛ / Ø25], sterilized 16541----------K
(Sterilized) BSS20-PE25 SLMPL25SS [0.22㎛ / Ø25], sterilized 6780-2502 [0.2㎛ / Ø25], sterilized 16541----------GUK, sterilized
SLGP033NS [0.22㎛ / Ø33] 6759-2502 [0.2㎛ / Ø25] 16541----------Q
[0.2㎛ / Ø28]
16533----------K
SLHPM33RS [0.45㎛ / Ø33], sterilized 6780-2504 [0.45㎛ / Ø25], sterilized 16533----------GUK, sterilized
BSS45-PE25 SLHP033NS [0.45㎛ / Ø33] 6759-2504 [0.45㎛ / Ø25] 16533----------Q
[0.45㎛ / Ø28]
1776B----------K
6786-1302 [0.2㎛ / Ø13], sterilized
BS20-NY13 SLGNX13NL [0.2㎛ / Ø13] 1776B----------Q
6789-1302 [0.2㎛ / Ø13]
[0.2㎛ / Ø15]
1776C----------K
BS45-NY13 SLHNX13NL [0.45㎛ / Ø13] 6789-1304 [0.45㎛ / Ø13] 1776C----------Q
[0.45㎛/ Ø15]
Nylon
17845----------R
BS20-NY25 SLGV033NS [0.2㎛ / Ø33] 6750-2502 [0.2㎛ / Ø25] 17845----------Q
[0.2㎛ / Ø25]
17846----------R
BS45-NY25 SLHV033NS [0.45㎛ / Ø33] 6750-2504 [0.45㎛ / Ø25] 17846----------Q
[0.45㎛ / Ø25]
• CA : Cellulose Acetate • MCE : Mixed Cellulose Ester • PES : Polyethersulfone • Nylon : Polyamide
235
BIOFACT CATALOGUE
BioFACT™ Syringe Filter selection guide
Maker Cat.No. Millipore Millex® Whatman® Sartorius Minisart®
* 17761----------K
6791-1302 [0.2㎛/ Ø13], sterilized
BS20-PV13 SLGV013SL [0.22㎛ / Ø13], sterilized * 17761----------Q
6779-1302 [0.2㎛/ Ø13]
[0.2㎛ / Ø15]
* 17762----------K
6791-1304 [0.45㎛/ Ø13], sterilized
BS45-PV13 SLHV013SL [0.45㎛ / Ø13], sterilized * 17762----------Q
6779-1304 [0.45㎛/ Ø13]
[0.45㎛ / Ø15]
PVDF
* 17764----------K
SLGV033NS [0.22㎛ / Ø33] * 17764----------S
BS20-PV25 6746-2502 [0.2㎛/ Ø25]
SLGV033RS [0.22㎛ / Ø33], sterilized * 17764----------Q
[0.2㎛ / Ø25]
* 17765----------K
SLHV033NS [0.45㎛ / Ø33] * 17765----------S
BS45-PV25 6746-2504 [0.45㎛/ Ø25]
SLHV033RS [0.45㎛ / Ø33], sterilized * 17765----------Q
[0.45㎛ / Ø25]
SLLGH13NL [0.2㎛ / Ø13]
BS20-PW13 - -
SLLG013SL [0.2 ㎛ / Ø13], sterilized
BS45-PW13 SLCR013NL [0.45㎛ / Ø13] - -
PTFE-W
BS20-PW25 SLLGH25NS [0.2㎛ / Ø25] - -
BS45-PW25 SLCR025NS [0.45㎛ / Ø25] - -
17573----------K
BSS20-PO13 SLFGX13NL [0.2㎛ / Ø13] 6784-1302 [0.2㎛ / Ø13]
[0.2㎛ / Ø15]
17574----------K
BSS45-PO13 SLFHX13NL [0.45㎛ / Ø13] 6784-1304 [0.45㎛ / Ø13]
[0.45㎛ / Ø15]
PTFE-O
17575----------K
BSS20-PO25 SLFG025NS [0.2㎛ / Ø25] 6784-2502 [0.2㎛ / Ø25]
[0.2㎛ / Ø25]
17576----------K
BSS45-PO25 SLFH025NS [0.45㎛ / Ø25] 6784-2504 [0.45㎛ / Ø25]
[0.45㎛ / Ø25]
• PVDF : Polyvinylidene Fluoride • PTFE-W : Polytetrafluoroethylene-Hydrophilic) • PTFE-O : Polytetrafluoroethylene-Hydrophobic)
236 BIOFACT CATALOGUE
Filtration
Membrane Filter [BIOFACT]
Various Materials, Pore Sizes and Diameters
BioFACT™ Membrane Filter is a microporous polymer lms with speci c pore size ratings and it guarantees high quality, repro-
ducibility and stability. BioFACT™ Membrane Filter is widely used for general sample ltration in various elds such as quality
control, environmental monitoring and research and it is especially suitable for microorganism / particle analysis and collection
due to its microporous feature. Customers can choose a wide variety of membrane materials, pore sizes and diameters according
to individual speci c needs.
Feature
• Suitable for microorganism / particle analysis and collection • Available to particle removal and ltration quicker than syringe lter
• through microporous feature • Various materials, pore sizes and diameters
Membrane lter How to use membrane lter
Feature of various membrane materials
Hydrophilic / Mild Hydrophilic / orgnic
Hydrophilic Mild organic solvent
orgnic solvent solvent (mid-polar)
PTFE
MCE PES PVDF Nylon
Type (Polytetra uoroethylene-
(Mixed Cellulose Ester) (Polyethersulfone) (Polyvinylidene Fluoride) (Polyamide)
Hydrophobic)
SEM image
Pore size 0.2㎛/0.45 ㎛ 0.2㎛/0.45 ㎛ 0.2㎛/0.45 ㎛ 0.2㎛/0.45 ㎛ 0.2㎛/0.45 ㎛
Polarity Hydrophilic (Polar) Polar / non-polar Hydrophobic (Non-polar)
Protein Binding High Low Very low Middle Low
Chemical compatibility Limited, pH 3.5 – 8 Limited, pH 1 – 14 High resistance High resistance Extremely high resistance
Research analysis Aqueous solutions (Culture Mild organic solvent & Organic solven filtration Organic solven filtration only
Application Remove of aqueous particles medium, additive and bu er) HPLC sample filtration Water Soluble & alkali solvent Air vent
Filter sterilization filtration filtration
237
BIOFACT CATALOGUE
Pore size selection on various ltration purpose Dimension size selection on di erent holder sizes
Pore Size 0.2 ㎛ 0.45 ㎛ Filter Holder Size Φ 25 Φ 47
Sterile- ltration, Particle Removal, Membrane Filter Diameter Φ 25 Φ 47
Purpose
Microbial Analysis Clari cation
Particle Size ＜ 3 ㎛ ＞ 3 ㎛
Feature of various membrane materials
PES MCE Nylon PVDF PTFE
Hydrophilic Hydrophilic & Hydrophobic Hydrophobic
Low protein binding High protein binding Low protein binding
Chemical compatibility
SUBSTRANCES MCE PES Nylon PVDF-W PTFE-O SUBSTRANCES MCE PES Nylon PVDF-W PTFE-O
Acids Halogenated Hydrocarbons
Acetic Acid ○ X X ○ ○ Carbon tetrachloride X △ △ ○ ○
Hydrochloric Acid ○ ○ X ○ ○ Chloroform ○ X △ ○ ○
Hydrochloric Acid (6N) X ○ X – ○ Ethylene Dichloride ○ X △ – –
Nitric Acid ○ – X ○ ○ Freon TF ○ ○ ○ ○ ○
Nitric Acid (6N) X – X ○ ○ Freon TMC ○ X △ – ○
Phosphoric Acid ○ – X – ○ Methylene Dichloride X X X – –
Sulfuric Acid ○ X X – X Perchloroethylene ○ △ – – –
Hydrofluoric Acid X – X X ○ Trichloroethylene ○ △ △ ○ ○
Bases Glycols
Ammonium Hydroxide (1N) ○ ○ ○ △ – Ethylene glycol – △ ○ ○ ○
Ammonium Hydroxide (3N) X ○ ○ – – Glycerol ○ △ ○ ○ ○
Potassium Hydroxide (3N) X ○ ○ ○ ○ Propylene Glycol – △ ○ – ○
Sodium Hydroxide (3N) X ○ ○ X ○ Ketones
Sodium Hydroxide (6N) X ○ ○ X ○ Acetone X X ○ X ○
Alcohols Cyclohexanone X X – X ○
Amyl Alcohol △ ○ ○ ○ ○ Methylethylketone – – △ X ○
Benzyl Alcohol (100%) X ○ ○ ○ ○ Methyl isobutylketone – X △ X ○
butyl alcohol △ ○ ○ ○ ○ Miscellaneous
Isopropyl alcohol △ ○ ○ ○ ○ Aniline – X △ – ○
Methanol △ ○ △ – ○ Di Methyl Formamide – X ○ X ○
Aromatic Hydrocarbons Formaldehyde (37%) – ○ ○ ○ ○
Benzene ○ – △ ○ ○ Gasoline ○ ○ △ ○ ○
Toluene ○ X X ○ ○ Hexane (dry) ○ △ – ○ ○
Xylene ○ X △ ○ ○ Petroleum – ○ – ○ ○
Ethers Phenol ○ X ○ ○ ○
Ethyl Ether ○ ○ X ○ ○ Pyridine X X △ X ○
Isopropyl Ether ○ – – – ○ Turpentine – ○ – – –
Dioxane X – ○ ○ ○ Water ○ ○ ○ ○ ○
Tetrahydrofuran X X X △ ○ Acetonitrile X ○ △ △ ○
Esters Sulfuric Acid Nickel Solution – – ○ – –
Amyl Acetate X – △ ○ ○
Butyl Acetate X – △ – ○ ※ ○ : Resistance △ : Limited resistance x : No resistance - : No data available
Cellosolve Acetate X ○ – – – • It is based on the material properties.
Ethyl Acetate X △ △ ○ ○ • It is recommended to use it for reference only.
Methyl Acetate X X △ X ○
Isopropyl Acetate X ○ – ○ –
238 BIOFACT CATALOGUE
Filtration
MCE (Mixed Cellulose Ester) Dimension (mm) Pore size (㎛) Cat.No. Size
• Material: Cellulose acetate & cellulose nitrate 0.2 ㎛ BM20-MC25 100 sheet / box
Φ 25
• Filtration of aqueous solution in research analysis 0.45 ㎛ BM45-MC25 100 sheet / box
0.2 ㎛ BM20-MC47 100 sheet / box
• Filtration of pure liquids / scattering measurements Φ 47
0.45 ㎛ BM45-MC47 100 sheet / box
Nylon (Polyamide)
• High adsorption & mechanical strength Dimension (mm) Pore size (㎛) Cat.No. Size
• High organic solvents and alkali liquids resistance 0.2 ㎛ BM20-NY25 100 sheet / box
Φ 25
• Broad chemical compatibility for solvents 0.45 ㎛ BM45-NY25 100 sheet / box
0.2 ㎛ BM20-NY47 100 sheet / box
• It is not recommended for filtration of bacteria culture, protein solution Φ 47
and other biological samples to prevent sample loss due to absorption. 0.45 ㎛ BM45-NY47 100 sheet / box
PES (Polyethersulfone)
Dimension (mm) Pore size (㎛) Cat.No. Size
• High heat / chemical resistance
0.2 ㎛ BM20-PE25 100 sheet / box
• Hydrophilic membrane filter with low protein adhesion Φ 25 0.45 ㎛ BM45-PE25 100 sheet / box
• Widely used for biological experiments 0.2 ㎛ BM20-PE47 100 sheet / box
Φ 47
• Filtration of aqueous solution, alkali liquid and alcohol media solvent 0.45 ㎛ BM45-PE47 100 sheet / box
PVDF (Polyvinylidene Fluoride)
• Excellent thermal and chemical resistance Dimension (mm) Pore size (㎛) Cat.No. Size
• Filtration for HPLC sample and solvent 0.2 ㎛ BM20-PV25 100 sheet / box
Φ 25
• Available to water soluble and lipoid soluble liquids 0.45 ㎛ BM45-PV25 100 sheet / box
0.2 ㎛ BM20-PV47 100 sheet / box
• Filtration of solutions containing protein, aqueous solution and Φ 47
mid organic solvent 0.45 ㎛ BM45-PV47 100 sheet / box
PTFE (Polytetra uoroethylene - Hydrophobic)
• Supported PTFE type with polypropylene net support on a thick Dimension (mm) Pore size (㎛) Cat.No. Size
PTFE-type membrane lter
0.2 ㎛ BM20-PT25 100 sheet / box
• Low friction / inadhesive and detachable hydrophobic filter Φ 25
0.45 ㎛ BM45-PT25 100 sheet / box
• Available to almost solvents 0.2 ㎛ BM20-PT47 100 sheet / box
Φ 47
• Thermally stable up to 145℃ 0.45 ㎛ BM45-PT47 100 sheet / box
239
BIOFACT CATALOGUE
BIOFACT CATALOGUE
Address. 2/3F, 240 EXPO-ro, Yuseong-gu, Daejeon,34124 Republic Korea
Tel. +82-70-7826-5453 (Korea : 1670-5695) Fax. 042-385-5511 E-mail [email protected]
Homepage www.bio-ft.com ｜ www.bio-ft.com/eng (Global)
Catalogue BF - QI - PCA - 01(2)
ⓒ BIOFACT, 2022
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