Description 18
Lyophilized PCR Enzyme
LyoFACT™ PCR Pre-Mix Lyophilisates 40
LyoFACT™ RT Pre-Mix Lyophilisates 42
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates 44
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (For Probe) 46
LyoFACT™ All in one PCR Pre-Mix Lyophilisates 48
Real-Time PCR Enzyme
Intercalating Dye BioFACT™ Real-Time PCR Series 56
SFCgreen® I 57
BioFACT™ Real-Time PCR Series (With SFCgreen® I) 59
BioFACT™ Real-Time PCR Series (With SYBR® Green I) 64
BioFACT™ Real-Time PCR Series (With EvaGreen™) 65
20X EvaGreen™ 66
BioFACT™ OneStep qRT-PCR Master Mix 67
FastFACT™ Real-Time PCR Master Mix 69
Probe BioFACT™ Multi-Star Real-Time PCR Master Mix 70
BioFACT™ OneStep Multi-Star qRT-PCR Series 72
FastFACT™ qPCR Master Mix 74
FastFACT™ qRT-PCR Master Mix 75
PCR Enzyme
Universal PCR BioFACT™ F-Star Taq DNA Polymerase (Hot Start) 78
(<3 kb, 5 kb) BioFACT™ Thumb Taq DNA Polymerase 80
BioFACT™ Taq DNA Polymerase 82
Gene Speci c PCR BioFACT™ H-Star Taq DNA Polymerase (Hot Start) 84
(<1 kb, 5 kb) BioFACT™ A-Star Taq DNA Polymerase (Hot Start) 86
Long PCR BioFACT™ Lamp Taq DNA Polymerase 88
(<40 kb, λ DNA 기준) BioFACT™ A-Star Lamp Taq DNA Polymerase (Hot Start) 90
High Fidelity PCR BioFACT™ Pfu DNA Polymerase 92
(<3 kb, <20 kb) BioFACT™ Lamp Pfu DNA Polymerase 94
Multiplex PCR (<1 kb BioFACT™ 2X Multi-Star PCR Series (Hot Start) 96
PCR Inhibition SimpleWay™ 2X PCR Master Mix III 98
RT & RT-PCR Enzyme
BioFACT™ RT Series 102
BioFACT™ OneStep RT-PCR Series 104
Isothermal Amplification
BioFACT™ Bst DNA Polymerase 108
Other Reagent
BioFACT™ Uracil DNA Glycosylase (UDG) 112
BioFACT™ dNTP and dNTP Set 113
5X Band Helper™ 114
BioFACT™ RNase Inhibitor 116
BioFACT™ RT Series
Synthesis of Long Length cDNA (<14 kb)
BioFACT™ RTase is a recombinant type of M-MLV reverse transcriptase(RNase H ) expressed in E. coli. It has greater rst-strand cDNA
-
yields than existing wild type of reverse transcriptase due to inhibition of RNA's secondary structure by its thermostable characteristic
at 50 ℃ for activation. BioFACT™ RT Kit is composed of BioFACT™ RTase, DTT, RNase-free water and optimized reaction bu er including
dNTPs for convenience. BioFACT™ 2X RT Pre-Mix is supplied as a ready-to-use, 2X concentrated Pre-Mix which includes an optimized
mixture of all the reagents (except primers and template) such as RTase, RNase inhibitor, DTT and dNTPs in a 0.2㎖ 8-strip tube.
Feature
• Thermostable and RNase H variant of M-MLV RTase (RNase H) - • Synthesis of long length cDNA (＜14 kb)
• Synthesize cDNA at 42℃ – 50℃ • Synthesis primer : random primer, oligo-d(T), gene speci c primer
• Highest activity at 50℃ • Great rst-strand cDNA yields
Application
• First-strand cDNA synthesis • Real-Time PCR
• RT-PCR • Primer extension analysis
QC DATA
- Comparison of cDNA synthesis efficiency on various RNA concentrations between BioFACT™ RT Kit and other companies' RT kits
(A) (B) (A) (B)
Total RNA Conc. ▶ 100 ng 20 ng 4 ng 0.8 ng 100 ng 20 ng 4 ng 0.8 ng Total RNA Conc. ▶ 100 ng 20 ng 4 ng 0.8 ng 100 ng 20 ng 4 ng 0.8 ng
M A B C D A B C D A B C D A B C D M M A B C D A B C D A B C D A B C D M M A B C D A B C D A B C D A B C D M M A B C D A B C D A B C D A B C D M
BIOFACT BIOFACT
Company A Company A
Company B Company B
Company C Company C
Target : Human total RNA, β-Actin (1 kb) gene Target : Human total RNA, BRCA2 (0.7 kb) gene
PCR Condition : 60°C, 35 cycles PCR Condition : 60°C, 35 cycles
Primer type : (A) Oligo-d(T) 20, (B) Random Hexamer Primer type : (A) Oligo-d(T) 20 , (B) Random Hexamer
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : cDNA 5 B : cDNA 5 C : cDNA 5 D : cDNA 5 -3 A : cDNA 5 B : cDNA 5 C : cDNA 5 D : cDNA 5 -3
0
-1
-2
-1
0
-2
102 BIOFACT CATALOGUE
DNA / RNA Amplification RT & RT-PCR Enzyme
Product Information
Standard Type
BioFACT™ RT-Kit, with RNase Inhibitor [BR122-10K]
▶Contents
BioFACT™ RTase (200 U/㎕)
RNase Inhibitor ( 40U/㎕)
5X RT Reaction Buffer (Including dNTP mixture)
8 mM DTT
RNase-free Water
▶Expiration date
1 Year and 6 months at -20 ± 5℃
1 Year and 6 months at -20 ± 5℃ (RNase Inhibitor)
Master Mix Type Pre-Mix Type
BioFACT™ 2X RT Master Mix, w/o dye [BR341-20h] BioFACT™ 2X RT Pre-Mix, w/o dye [BR441-096]
▶Contents ▶Contents
2X RT Master Mix [1 ㎖ x 2 ea] 2X RT Pre-Mix [96 tubes]
▶Expiration date ▶Expiration date
1 Year at -20 ± 5℃ 1 Year at -20 ± 5℃
Type Cat.No. Product Size
BioFACT™ RT-Kit (M-MLV, RNase H-), RTase, 5X Reaction Bu er (include each 10mM dNTP), 8 mM DTT,
BR122-10k 10,000 U (200 U/㎕)
Standard RNase Inhibitor
BR123-10k BioFACT™ RT-Kit (M-MLV, RNase H-), RTase, 5X Reaction Bu er (include each10mM dNTP), 8 mM DTT 10,000 U (200 U/㎕)
Master Mix BR341-20h BioFACT™ 2X RT Master Mix, w/o dye 1 ㎖ x 2 ea
Pre-Mix BR441-096 BioFACT™ 2X RT Pre-Mix, w/o dye, total 20㎕ reactions 96 Tubes (8 Strip x 12 ea)
RI152-20h BioFACT™ RNase Inhibitor 2,000 U (40 U/㎕)
103
BIOFACT CATALOGUE
BioFACT™
OneStep RT-PCR Series ⓗ
Fast and Convenient OneStep RT-PCR Reaction in a Single Tube
BioFACT™ OneStep RT-PCR Series is designed for convenience and high e ciency in carrying out RT-PCR in a single tube at one step.
Included BioFACT™ RTase and BioFACT™ H-Star Taq DNA Polymerase is blended in one mixture with best optimized rate to simplify
reaction step and reducing the possibility of contamination. BioFACT™ OneStep RT-PCR Series has great rst-strand cDNA yields by
its thermostable characteristic at 50℃ for activation of BioFACT™ RTase and it allows robust, high speci c ampli cation by BioFACT™
H-Star Taq DNA Polymerase included in the mixture. It is optimized to amplify less than 1 kb size target.
Feature
• Thermostable and RNase H variant of M-MLV RTase (RNase H ) - • Chemical mediated Hot Start enzyme
• Synthesize cDNA at 42℃ – 50℃ (highest activity at 50℃) • Great e ciency & high speci city
• Synthesis of long length cDNA (＜14 kb) • Easy sample optimization
• Synthesis gene size : < 1 kb PCR • Low-copy transcripts ampli cation
• Synthesis primer : gene speci c primer
Application
• RT-PCR
QC DATA
- Comparison of amplification efficiency on various RNA concentrations between BioFACT™ OneStep RTPCR Kit
and other companies' OneStep RT-PCR Kits
β-Actin (1 kb) BRCA2 (0.7 kb) β-Actin (1 kb) TNFR1 (0.6 kb) BRCA2 (0.4 kb)
M A B C D E F M M A B C D E F M M A B C D M M A B C D M M A B C D M
BIOFACT BIOFACT
Company A Company A
Company B Company B
Target : Human Total RNA PCR Condition : 60°C, 40 cycles Target : Human Total RNA PCR Condition : 60°C, 40 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 100 ng B : 20 ng C : 4 ng D : 0.8 ng E : 0.16 ng F : 0.032ng A : 100 ng B : 10 ng C : 1 ng D : 0.1 ng
104 BIOFACT CATALOGUE
DNA / RNA Amplification RT & RT-PCR Enzyme
Product Information
Standard Type
BioFACT™ OneStep RT-PCR Kit ⓗ [BR861-050]
▶Contents
BioFACT™ OneStep RT-PCR Enzyme Mixture (RTase + H-Star Taq DNA Polymerase)
5X OneStep RT-PCR Reaction Buffer (Including dNTP mixture)
5X Band Helper™
RNase-free Water
▶Expiration date
1 Year at -20 ± 5℃
Master Mix Type Pre-Mix Type
BioFACT™ 2X OneStep RT-PCR Master Mix ⓗ [BR331-50h] BioFACT™ 2X OneStep RT-PCR Pre-Mix ⓗ [BR431-096]
▶Contents ▶Contents
2X OneStep RT-PCR Master Mix [1 ㎖ x 5 ea] 2X OneStep RT-PCR Pre-Mix [96 tubes]
▶Expiration date ▶Expiration date
1 Year at -20 ± 5℃ 1 Year at -20 ± 5℃
Type Cat.No. Product Size
BioFACT™ One Step RT-PCR Kit ⓗ, Enzyme mixture, 5X Reaction Bu er (Including 10mM dNTP),
BR861-050 50 reaction
5X Band Helper™
Standard
BR861-100 BioFACT™ One Step RT-PCR Kit ⓗ, Enzyme mixture, 5X Reaction Bu er (Including 10mM dNTP), 100 reaction
5X Band Helper™
BR331-10h BioFACT™ 2X OneStep RT-PCR Master Mix ⓗ, with dye 1 ㎖ x 1 ea
Master Mix
BR331-50h BioFACT™ 2X OneStep RT-PCR Master Mix ⓗ, with dye 1 ㎖ x 5 ea
Pre-Mix BR431-096 BioFACT™ 2X OneStep RT-PCR Pre-Mix ⓗ, with dye, total 30㎕ reactions 96 Tubes (8 strip x 12 ea)
* Please Contact us about PCR Master Mix or Pre-Mix without dye.
105
BIOFACT CATALOGUE
DNA / RNA
Amplification
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
Description 18
Lyophilized PCR Enzyme
LyoFACT™ PCR Pre-Mix Lyophilisates 40
LyoFACT™ RT Pre-Mix Lyophilisates 42
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates 44
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (For Probe) 46
LyoFACT™ All in one PCR Pre-Mix Lyophilisates 48
Real-Time PCR Enzyme
Intercalating Dye BioFACT™ Real-Time PCR Series 56
SFCgreen® I 57
BioFACT™ Real-Time PCR Series (With SFCgreen® I) 59
BioFACT™ Real-Time PCR Series (With SYBR® Green I) 64
BioFACT™ Real-Time PCR Series (With EvaGreen™) 65
20X EvaGreen™ 66
BioFACT™ OneStep qRT-PCR Master Mix 67
FastFACT™ Real-Time PCR Master Mix 69
Probe BioFACT™ Multi-Star Real-Time PCR Master Mix 70
BioFACT™ OneStep Multi-Star qRT-PCR Series 72
FastFACT™ qPCR Master Mix 74
FastFACT™ qRT-PCR Master Mix 75
PCR Enzyme
Universal PCR BioFACT™ F-Star Taq DNA Polymerase (Hot Start) 78
(<3 kb, 5 kb) BioFACT™ Thumb Taq DNA Polymerase 80
BioFACT™ Taq DNA Polymerase 82
Gene Speci c PCR BioFACT™ H-Star Taq DNA Polymerase (Hot Start) 84
(<1 kb, 5 kb) BioFACT™ A-Star Taq DNA Polymerase (Hot Start) 86
Long PCR BioFACT™ Lamp Taq DNA Polymerase 88
(<40 kb, λ DNA 기준) BioFACT™ A-Star Lamp Taq DNA Polymerase (Hot Start) 90
High Fidelity PCR BioFACT™ Pfu DNA Polymerase 92
(<3 kb, <20 kb) BioFACT™ Lamp Pfu DNA Polymerase 94
Multiplex PCR (<1 kb BioFACT™ 2X Multi-Star PCR Series (Hot Start) 96
PCR Inhibition SimpleWay™ 2X PCR Master Mix III 98
RT & RT-PCR Enzyme
BioFACT™ RT Series 102
BioFACT™ OneStep RT-PCR Series 104
Isothermal Amplification
BioFACT™ Bst DNA Polymerase 108
Other Reagent
BioFACT™ Uracil DNA Glycosylase (UDG) 112
BioFACT™ dNTP and dNTP Set 113
5X Band Helper™ 114
BioFACT™ RNase Inhibitor 116
BioFACT™
Bst DNA Polymerase
DNA Polymerase for Isothermal Ampli cation
BioFACT™ Bst DNA Polymerase is a recombinant protein lacking the 5'→3' exo-nuclease activity of Bacillus stearothermophilus DNA
Polymerase. The optimum conditions for the product are 65℃, 30 to 60 minutes, and it can be applied to Loop-Mediated isothermal
ampli cation (LAMP) PCR with isothermal ampli cation technology.
Feature
• Synthesis primer : gene speci c primer • Lacking 5´→3´ exonuclease activity
• Suitable for applications requiring thermophilic strand displacement • 5' → 3' exonuclease activity : No
• Optimization activity at 65℃ • 3' → 5' exonuclease activity ( delity) : No
• Relative activity 80~90% at 60℃, 65~75% at 55℃, 15~20% at 50℃
Application
• Loop-mediated isothermal ampli cation(LAMP) • Whole genome ampli cation
• Isothermal ampli cation • RT-LAMP
QC DATA
- Comparison of LAMP PCR activity by concentration of enzyme between BioFACT™ Bst DNA Polymerase and other company’s
polymerase
Company A BIOFACT Company A BIOFACT
M A B C D E F G H M A B C D E F G H M A B C D E F G H M A B C D E F G H
Target : Human Actin Target : Human Chromosome 22
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 8U B : 4U C : 2U D : 1U E : 0.5U F : 0.25U G : 0.125U H : NTC A : 8U B : 4U C : 2U D : 1U E : 0.5U F : 0.25U G : 0.125U H : NTC
108 BIOFACT CATALOGUE
DNA / RNA Amplification Isothermal Amplification
BioFACT™ - Comparison of sensitivity according to various template concentrations between BioFACT™ Bst DNA Polymerase
and other companies’ polymerases
Bst DNA Polymerase
<BIOFACT> < Company A>
10 0
10 -1
10 -2 10 0
10 -3
10 -4 10 -1
10 -2
< Company B> < Company C>
10 0
10 -1 10 0
10 -1
10 -2
10 -3 10 -2
Target : human actin [FAM]
△Ct Value 10 0 10 -1 10 -2 10 -3 10 -4 10 -5
BIOFACT 7.15 14.92 20.4 25.75 31.52 33.6
Company A 15.53 32.19 49.91 N/A N/A N/A
Company B 4.10 19.04 38.58 55.49 N/A N/A
Company C 14.93 37.18 56.88 N/A N/A N/A
Product Information
Standard Type
BioFACT™ Bst DNA Polymerase , Large Fragment [BP101-16h]
▶Contents
BioFACT™ Bst DNA Polymerase, Large Fragment (8 unit/㎕)
10X Bst Reaction Bu er
Each 10 mM dNTP Mix
▶Expiration date
1 Year and 6 months at -20 ± 5℃
Cat.No. Product Size
BP101-16h BioFACT™ Bst DNA Polymerase, Large Fragment 1,600 U (8 U/㎕)
BP101-80h BioFACT™ Bst DNA Polymerase, Large Fragment 8,000 U (1,600 U x 5 ea)
109
BIOFACT CATALOGUE
DNA / RNA
Amplification
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
Description 18
Lyophilized PCR Enzyme
LyoFACT™ PCR Pre-Mix Lyophilisates 40
LyoFACT™ RT Pre-Mix Lyophilisates 42
LyoFACT™ OneStep RT-PCR Pre-Mix Lyophilisates 44
LyoFACT™ OneStep qRT-PCR Pre-Mix Lyophilisates (For Probe) 46
LyoFACT™ All in one PCR Pre-Mix Lyophilisates 48
Real-Time PCR Enzyme
Intercalating Dye BioFACT™ Real-Time PCR Series 56
SFCgreen® I 57
BioFACT™ Real-Time PCR Series (With SFCgreen® I) 59
BioFACT™ Real-Time PCR Series (With SYBR® Green I) 64
BioFACT™ Real-Time PCR Series (With EvaGreen™) 65
20X EvaGreen™ 66
BioFACT™ OneStep qRT-PCR Master Mix 67
FastFACT™ Real-Time PCR Master Mix 69
Probe BioFACT™ Multi-Star Real-Time PCR Master Mix 70
BioFACT™ OneStep Multi-Star qRT-PCR Series 72
FastFACT™ qPCR Master Mix 74
FastFACT™ qRT-PCR Master Mix 75
PCR Enzyme
Universal PCR BioFACT™ F-Star Taq DNA Polymerase (Hot Start) 78
(<3 kb, 5 kb) BioFACT™ Thumb Taq DNA Polymerase 80
BioFACT™ Taq DNA Polymerase 82
Gene Speci c PCR BioFACT™ H-Star Taq DNA Polymerase (Hot Start) 84
(<1 kb, 5 kb) BioFACT™ A-Star Taq DNA Polymerase (Hot Start) 86
Long PCR BioFACT™ Lamp Taq DNA Polymerase 88
(<40 kb, λ DNA 기준) BioFACT™ A-Star Lamp Taq DNA Polymerase (Hot Start) 90
High Fidelity PCR BioFACT™ Pfu DNA Polymerase 92
(<3 kb, <20 kb) BioFACT™ Lamp Pfu DNA Polymerase 94
Multiplex PCR (<1 kb BioFACT™ 2X Multi-Star PCR Series (Hot Start) 96
PCR Inhibition SimpleWay™ 2X PCR Master Mix III 98
RT & RT-PCR Enzyme
BioFACT™ RT Series 102
BioFACT™ OneStep RT-PCR Series 104
Isothermal Amplification
BioFACT™ Bst DNA Polymerase 108
Other Reagent
BioFACT™ Uracil DNA Glycosylase (UDG) 112
BioFACT™ dNTP and dNTP Set 113
5X Band Helper™ 114
BioFACT™ RNase Inhibitor 116
BioFACT™ Uracil DNA
Glycosylase For PCR Diagnosis
Elimination of Carry-Over Contamination
BioFACT™ Uracil-DNA Glycosylase is useful to reduce the carry-over contamination in PCR based diagnostics. In UDG system, PCR
is performed using dUTP through substitution of dTTP and post-PCR is performed after UDG treatment. If there is contamination
of PCR product in the previous step, by the UDG system, contaminated DNA is eliminated through hydrolysis of N-glycosylic bond
between Uracil and template in PCR product. It can't be used for PCR enzymes which have proofreading activity such as BioFACT™
Pfu DNA Polymerase and BioFACT™ Lamp Taq DNA Polymerase.
Feature
• Source : E.coli (K-12 strain)
• Removing uracil-containing DNA
• Elimination of carry-over contamination
Application
• Diagnosis system using PCR
Product Information
BioFACT™ Uracil-DNA Glycosylase [UD121-10h]
▶Contents
Uracil-DNA Glycosylase (1 U/㎕)
▶Expiration date
2 Year and 3 months at -20 ± 5℃
Type Cat.No. Product Size
BIOFACT UD121-10h BioFACT™ Uracil-DNA Glycosylase 1,000 U
112 BIOFACT CATALOGUE
DNA / RNA Amplification Other Reagent
BioFACT™
dNTP and dNTP Set
High Quality, High Reproducibility
BioFACT™ dNTP and dNTP Set is manufactured through the enzyme synthesis process. Which helps higher PCR ampli cation e -
ciency compared to products manufactured by organic synthesis. In case of BioFACT™ dNTP Mix, it contains aqueous solution of
dATP, dCTP, dGTP and dTTP, each at a nal concentration of 25 mM. The Mix o ers the possibility to reduce the number of pipetting
steps and the risk of reaction set up errors.
Application
• High efficiency for all PCR amplifications • Real-Time PCR
• Long PCR • Stable for quantitative PCR
- Purity - Comparison of GLO gene amplification efficiency by various sizes
and dNTP concentrations
HPLC plot of purified dTTP BIOFACT Company A Company B
Purity : >99%
M A B C D E M M A B C D E M M A B C D E M
mAU PurePeak dNTP HPLC analysis
DNTP Show >99% triphosphate GLO
purity with
2000 negligible di-and (1 kb)
monophosphate and
1500
tetraphosphate forms.
1000
DNDP GLO
500 DNTeP
0 (3.6 kb)
4 6 8 10 12 min
RT Height Area Area,
[min] [mAU] [mAU*s] %
GLO
(10 kb)
DNDP 2.123 9.396 0.117
DNTP 1668.474 8031.388 99.824
DNTeP 1.223 4.800 0.060
GLO
(14 kb)
Product Information
BioFACT™ Lamp Taq DNA Polymerase was used for this comparison test.
M : 1kb DNA Ladder A : 2.5 mM B : 5 mM C : 10 mM D : 15 mM E : 20 mM
▶Expiration date
2 Year and 3 months at -20 ± 5℃
Maker Cat.No. Product Size
DN112-10h BioFACT™ dNTP Mix (each 10 mM) 1 ㎖
DN118-10h BioFACT™ dNTP(U) Mix (each 10 mM) 1 ㎖
DN101-10h BioFACT™ dATP (100 mM) 1 ㎖
DN102-10h BioFACT™ dGTP (100 mM) 1 ㎖
BIOFACT DN103-10h BioFACT™ dCTP (100 mM) 1 ㎖
DN104-10h BioFACT™ dTTP (100 mM) 1 ㎖
DN105-10h BioFACT™ dUTP (100 mM) 1 ㎖
DN106-40h BioFACT™ dNTP Set (each 100 mM) 1 ㎖ x 4 ea
DN107-40h BioFACT™ dNTP(U) Set (each 100 mM) 1 ㎖ x 4 ea
113
BIOFACT CATALOGUE
5X Band Helper™
PCR Additive for Di cult PCR
5X Band Helper™ is an innovative PCR additive which is highly suitable for use in all PCR enzymes of BIOFACT.
It enables e cient ampli cation and improved results can be obtained by adjusting of 5X Band Helper™ in the following cases.
* Di cult templates which are GC-rich or have a high degree of secondary structure
* Presence of non speci c bands or products
* Please refer to the protocol of PCR Enzymes to check the recommended concentrations of 5X Band Helper™.
It is recommended to use a minimum amount of 5X Band Helper™ in case of PCR enzymes that have delity features.
Application
• PCR additive for optimal PCR reaction set up
• Increase of ampli cation e ciency using BioFACT™ PCR enzymes
QC DATA
- Comparison of PCR amplification efficiency on various concentrations of 5X Band Helper™
DRD4 TNFR1
M A B C D E M M A B C D E M
NF-kB ApoE
A B C D E A B C D E
M M M M
Template : Human genomic DNA 10 ng PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder A : 0X Band Helper™ B : 0.5X Band Helper™ C : 1X Band Helper™ D : 1.5X Band Helper™ E : 2X Band Helper™
Optimal concentration of 5X Band Helper™ may differ depending on various target genes.
※ PCR was performed using BioFACT™ Taq DNA Polymerase.
114 BIOFACT CATALOGUE
DNA / RNA Amplification Other Reagent
- Comparison of gradient PCR efficiency on various concentrations of 5X Band Helper™
5X Band Helper™
BH 0X BH 0X
DRD4 SOX (1 kb)
M 1 2 3 4 5 6 7 8 9 10 11 12 M M 1 2 3 4 5 6 7 8 9 10 11 12 M
BH 1X BH 1.5X
DRD4 SOX (1 kb)
M 1 2 3 4 5 6 7 8 9 10 11 12 M M 1 2 3 4 5 6 7 8 9 10 11 12 M
Template : Human genomic DNA 10 ng PCR Condition : 35 cycles M : 1kb DNA Ladder
1: 50.0℃ 2: 50.3℃ 3: 51.2℃ 4: 52.7℃ 5: 54.7℃ 6: 57.2℃ 7: 60.2℃ 8: 62.9℃ 9: 64.9℃ 10: 66.6℃ 11: 67.5℃ 12: 68.0℃
※ PCR was performed using BioFACT™ Taq DNA Polymerase.
Set-up of 5X Band Helper™
Please perform PCR ampli cation by adjusting the nal concentration of 5X Band Helper™ from 0.5X to 2X and choose the optimal
concentration of 5X Band Helper™.
How to use Band Helper™ Product Information
5X Band Helper™ [BB741-10k]
Reaction mixture Mix Ⅰ Mix Ⅱ Mix Ⅲ Mix Ⅳ Mix Ⅴ
(conc. of 5X BandHelper™) (0X) (0.5X) (1X) (1.5X) (2X)
10X Taq Reaction Bu er 5 ㎕ 5 ㎕ 5 ㎕ 5 ㎕ 5 ㎕
Each 10 mM dNTP mix 1 ㎕ 1 ㎕ 1 ㎕ 1 ㎕ 1 ㎕
Primer F (10 pmole /㎕) 2 ㎕ 2 ㎕ 2 ㎕ 2 ㎕ 2 ㎕
Primer R (10 pmole /㎕) 2 ㎕ 2 ㎕ 2 ㎕ 2 ㎕ 2 ㎕
Template DNA - ㎕ - ㎕ - ㎕ - ㎕ - ㎕
5X Band Helper™ 0 ㎕ 5 ㎕ 10 ㎕ 15 ㎕ 20 ㎕
Taq (5 U/㎕) 0.25 ㎕ 0.25 ㎕ 0.25 ㎕ 0.25 ㎕ 0.25 ㎕ ▶Contents ▶Expiration date
Add D.W to 50 ㎕ 50 ㎕ 50 ㎕ 50 ㎕ 50 ㎕ 5X Band Helper™ 2 Year and 3 months at -20 ± 5℃
Maker Cat.No. Product Size
BIOFACT BB741-10k 5X Band Helper™ 1 ㎖ × 10 ea
115
BIOFACT CATALOGUE
BioFACT™ RNase Inhibitor
E cient Protection of RNA from Ribonuclease
BioFACT™ RNase Inhibitor is a 91 KDa recombinant protein derived from Rattus norvegicus. It inhibits RNase activity through a 1:1
non-covalent binding with RNase A, B and C, and does not a ect the activity of RNase H, T1, S1 nuclease, SP6 RNA polymerase, T7 RNA
polymerase, T3 RNA polymerase, AMV reverse transcriptase, M-MLV Reverse transcriptase, and Taq DNA polymerase.
BioFACT™ RNase Inhibitor is useful for protecting RNA from ribonuclease during cDNA synthesis, one-step RT-PCR, RNA synthesis, and
RNA preparation experiments.
Feature
• 1 Unit : Activity that inhibits 50% of the activity of 5 ng of RNase A • Molecular weight : 91 kDa Protein
• Source : An E.coli strain with a recombinant RNase Inhibitor gene • Heat inactivation : 70 ℃ for 10 min
from Rattus • Optimal pH range : pH 5.5 ~ 9.0
Application
• Isolation and puri cation of RNA • In vitro transcription/translation reactions
• RT-PCR reactions • RNase protection assays
• cDNA synthesis reactions • RNA-dependent in vitro functional assays
QC DATA
- Comparison of LOD between BioFACT™ RNase Inhibitor and other company’s RNase Inhibitor
(Concentration of RNase A is fixed to 1.6 ng)
Company A Company A
M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M
BIOFACT BIOFACT
M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M
RNase Inhibitor RNase A
1 : 4 unit 2 : 2 unit 3 : 1 unit 4 : 0.5 unit 5 : 0.25 unit 6 : 0.13 unit 1 : 1600 pg 2 : 800 pg 3 : 400 pg 4 : 200 pg 5 : 100 pg 6 : 50 pg
116 BIOFACT CATALOGUE
DNA / RNA Amplification Other Reagent
Product Information
BioFACT™ RNase Inhibitor [RI152-20h]
▶Contents
BioFACT™ RNase Inhibitor ( 40U/㎕)
▶Expiration date
1 Year and 6 months at -20 ± 5℃
Maker Cat.No. Product Size
BIOFACT RI152-20h BioFACT™ RNase Inhibitor 2,000 U (40 U/㎕)
117
BIOFACT CATALOGUE
DNA / RNA
Preparation
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
DNA Prep Kit Description 120
Magnetic Bead / Stand / Pipettor
DaBead™ Magnetic Bead 124
DaBead™ Magnetic Separation Stand 126
DaBead™ Magnetic Bead Press Pipettor 128
DaBead™ Magnetic Bead Pipettor 130
Viral RNA/DNA Extraction
DaBead™ Viral RNA/DNA Prep Kit [Magnetic Bead Type] 132
HiGene™ Viral RNA/DNA Prep Kit [Column Type] 136
Genomic DNA Extraction
DaBead™ Genomic DNA Prep Kit For Bacterium, Cultured cell, Whole Blood, 138
Animal Tissue, Saliva, Oral Epithelial Cell, Hair Root [Magnetic Bead Type]
DaBead™ Genomic DNA Prep Kit For Plant [Magnetic Bead Type] 143
DaBead™ GMO DNA Extraction Kit I For Potato, Bean, Maize [Magnetic Bead Type] 148
DaBead™ Genomic DNA Extraction Kit For Rice [Magnetic Bead Type] 151
DaBead™ Cell Free DNA Prep Kit For Plasma, Serum [Magnetic Bead Type] 153
HiGene™ Genomic DNA Prep Kit [Column Type] 155
HiGene™ Genomic DNA Prep Kit For Microorganisms [Grinding Bead & Column Type] 159
HiGene™ Genomic DNA Prep Kit For Soil [Grinding Bead & Column Type] 162
HiGene™ Genomic DNA Prep Kit [Solution Type] 165
SimpleWay™ gDNA Prep & PCR Set [Easy Type] 169
Total RNA Extraction
DaBead™ Total RNA Prep Kit [Magnetic Bead Type] 171
HiGene™ Total RNA Prep Kit [Column Type] 174
Plasmid Extraction
DaBead™ Plasmid Prep Kit [Magnetic Bead Type] 177
HiGene™ Plasmid Mini Prep Kit (Ver.2.0) [Column Type] 180
PCR Product Purification
DaBead™ PCR Puri cation Kit [Magnetic Bead Type] 182
DaBead™ Size-Selective Cleanup Kit [Magnetic Bead Type] 186
DaBead™ Gel Extraction Kit [Magnetic Bead Type] 188
DaBead™ Sequencing Reaction Clean Up Kit [Magnetic Bead Type] 190
HiGene™ Gel & PCR Puri cation System [Column Type] 192
Know-how for Preparation
1. It is recommended to refer to the following method to prevent the cap of tube from being broken during elution step
(In case of extraction using column typed kits).
* Please cross the caps of 1.5㎖ tubes when placing them into the centrifuge.
2. Please use the optimal amount of fresh sample for the extraction.
3. Make washing bu er fresh before starting the experiment.
4. It is recommended to grind the sample at low temperature to minimize damages to the sample.
Grinding in a mortar with liquid nitrogen Grinding using sample preparation system (grinding machine)
5. Please refer to the QC DATA page of each product to get more know-how for preparation.
120 BIOFACT CATALOGUE
DNA / RNA Preparation
DaBead™ Magnetic Bead
Magnetic Nanoparticle
Magnetic nanoparticle is a small bead particle which has magnetic property and it can speci cally bind to nucleic acid or
protein through its speci c functional group. It is a spotlighted technology in a variety of bio-technology applications such as
DNA/RNA/Protein extraction due to its superior binding and dissociation features though magnetic property. DaBead™ Mag-
netic Bead is coated with silica on the surface of superparamagnetic nanoparticles and it can be conveniently used for purifying
nucleic acids such as genomic DNA, cell free DNA, total RNA, plasmid DNA, or DNA fragment.
Advantages of Magnetic Bead Preparation Kits
• Uniformity in bead size • Minimization of aggregation between Magnetic Beads
• Prevention of toxic exposure to iron • Two types of bead sizes: Ø110nm and Ø
Preparation Step using Magnetic Bead
Magnetic
Cell line Bead
Bacteria Magnet Magnet
Plasmid
Samples Lysis Binding and Washing DNA / RNA elution
BIOFACT product
• DaBead™ Magnetic Bead Series
121
BIOFACT CATALOGUE
Selection GuideⅠ
(Genomic DNA Prep Kit)
DNA Quality
C
S M
E
DNA Yield Small volume sample
M S Long fragment
S
C C M
E E
S
C M Magnetic Bead Type
M
E C S Solution Type
C Column Type
S E
M E Easy Type
Scale up Speed
Minimum handling error
Magnetic Bead Type Solution Type Column Type Easy Type
DNA yield ＊＊＊ ＊＊＊ ＊＊ ＊
DNA quality ＊＊ ＊＊ ＊＊＊ ＊
Speed ＊＊ ＊ ＊＊ ＊＊＊
Minimum handling error ＊＊ ＊ ＊＊ ＊＊＊
Small volume of sample ＊＊＊ ＊＊＊ ＊＊ ＊
Scale up ＊＊＊ ＊＊＊ ＊ ＊＊
122 BIOFACT CATALOGUE
DNA / RNA Preparation
Selection GuideⅡ
(Genomic DNA Prep Kit)
* The color of the solution in the process is for better understanding only.
Magnetic Bead Type Easy Type Solution Type Column Type
Cell lysis Cell lysis Cell Lysis & Precipitation Samples
Cell lysis
Cell lysis Cell lysis Cell lysis Cell Lysis & Precipitation Cell Lysis & Precipitation Samples
Samples
Cell line Bacteria Plasmid
Cell lysis Cell lysis Cell Lysis & Precipitation Samples Cell line Bacteria Plasmid
Cell line Bacteria Plasmid
Lysis
Protein Precipitation Magnetic Bead Biniding Cell line Bacteria Plasmid Lysis Lysis
Protein Precipitation Protein Precipitation Magnetic Bead Biniding Magnetic Bead Biniding
Lysis
Boiling 10min Protein Precipitation Magnetic Bead Biniding
75℃ 15min(act.) Boiling 10min
95℃ 5min(inact.) Boiling 10min
Column Binding Magnetic Bead Magnetic 75℃ 15min(act.) 75℃ 15min(act.)
Washing & Dry Bead 95℃ 5min(inact.)
DNA Precipitation Boiling 10min Column Binding 95℃ 5min(inact.) Magnetic Bead Magnetic Bead Magnetic
Column Binding
Magnetic
Bead
75℃ 15min(act.)
DNA Precipitation DNA Precipitation Washing & Dry Washing & Dry Bead
95℃ 5min(inact.)
Column Binding Magnetic Bead Magnetic
Preparation DNA Precipitation Washing & Dry Bead
Magnet
step Magnet Magnet
Binding and Washing
Washing DNA Elution
Washing Magnet Binding and Washing Binding and Washing
Washing Washing DNA Elution DNA Elution
Washing Washing
Binding and Washing
Magnet
Washing DNA Elution Magnet
Washing Magnet
DNA / RNA elution Magnet
DNA Elution DNA Elution
DNA / RNA elution DNA / RNA elution
Magnet
DNA Elution
DNA Elution DNA Elution DNA Elution Magnet
DNA / RNA elution
Magnet
Magnet
Eluted DNA DNA Elution DNA Elution Magnet Magnet Magnet
Eluted DNA Eluted DNA
Magnet
Eluted DNA
- Salting-out method
- Scale-up using a single kit - Quick extraction in large quantities
- Efficient extraction from small - Efficient extraction from
- Convenient extraction of gDNA of samples
Features quantity of sample various samples
from large quantities of samples - Simple and quick extraction process
- High-concentration gDNA - High-quality gDNA extraction
- High-concentration gDNA extraction - Difficulty in long-term storage
extraction
Extraction 30 mins. – 1 hour 30 min. 1 hour and 30 mins. 30 – 45 mins.
time
Dry
Magnetic Bead Cell Lysis Magnetic Bead Washing Magnetic Bead Elution
Binding
Type
Easy Type Add Bu er Boiling
DNA
Protein
Solution Type Cell Lysis Precipitation Precipitation Washing Elution
Cell Lysis Column Washing Dry Elution
Column Type Binding membrane
123
BIOFACT CATALOGUE
DaBead™ Magnetic Bead
Magnetic Bead Synthesized with In-House Technology
DaBead™ Magnetic bead is a product developed for nucleic acid puri cation by coating the surface of Super Paramagnetic Nano-
particle with silica. It allows for convenient nucleic acid extraction based on the principle that nucleic acid speci cally binds to the
surface of small magnetic beads. There are magnetic beads for DNA and RNA in di erent sizes.
Feature
• Uniform bead size • Minimization of aggregation between beads
• High binding affinity • Prevention of toxic iron exposure through polymer Shell
• Various types & sizes of samples purification • Automated extraction equipment for diagnostic purpose available
• Affordable price through in-house development and synthesis • Various bead types suitable for multiple purposes
Typical SEM, TEM images of silica coated superparamagnetic nanoparticles
< EDS analysis of Magnetic Bead > Real image
100 nm
Enlargement image
Fe, Si overlay image Fe image Si image
Silica
100nm 100 nm 100 nm
Fe K Si K
JED-2300 AnalysisStation
124 BIOFACT CATALOGUE
DNA / RNA Preparation
Antibodies
Immunoassay Silica Coated
Infectious Disease Test Nucleic acid (DNA/RNA)
Superpara-
magnetic
Nanoparticle
DNA probes Fluorescence labeling
SNPs analysis / Gene diagnostic test DNA analysis (Fluorescent bar-corded)
Product Information
DaBead™ Magnetic Bead Si - I (Ø110㎚) DaBead Magnetic Bead Si - II (Ø150㎚)
Maker Cat.No. Product Size
MB701-50h DaBead™ Magnetic Bead Si - I 5 ㎖
MB701-25k DaBead™ Magnetic Bead Si - I 25 ㎖
MB701-100k DaBead™ Magnetic Bead Si - I 100 ㎖
MB701-500k DaBead™ Magnetic Bead Si - I 500 ㎖
MB739-50h DaBead™ Magnetic Bead Si - II 5 ㎖
BIOFACT MB739-25k DaBead™ Magnetic Bead Si - II 25 ㎖
MB739-100k DaBead™ Magnetic Bead Si - II 100 ㎖
MB739-500k DaBead™ Magnetic Bead Si - II 500 ㎖
MB712-50h DaBead™ Magnetic Bead Si (RNA) 5 ㎖
MB712-25k DaBead™ Magnetic Bead Si (RNA) 25 ㎖
MB712-100k DaBead™ Magnetic Bead Si (RNA) 100 ㎖
125
BIOFACT CATALOGUE
Patent registration number : No. 10-1905136 / No. 10-1942097
DaBead™
Magnetic Separation Stand
Any Scale of Bead Extraction Available in One Stand
DaBead™ Magnetic Separation Stand is designed for magnetic bead extraction in any scale (mini, midi and maxi prep) in one stand.
Both 1.5㎖ tube and 50㎖ tube can be used in one stand for puri cation and it can be compatible with various 1.5㎖ tubes through
its rubber packing on the stand. In addition, DaBead™ Magnetic Separation Stand for 96well is designed to be used for both 96 well
plates and 8 strip tubes.
Feature
• Any scale of bead extraction available in one stand • Available to use for 96 well plate & 8 strip tubes (SJ1-MSS96)
• Superior compatibility with various 1.5㎖ tubes (SJ-MSS11) • Large number of sample preparation available at once (SJ1-MSS96)
• Strong magnetic strength (SJ1-MSS11)
Related Product
• Genomic DNA Prep Kit • Cell Free DNA Prep Kit • PCR Purification Kit • Size-Selective Cleanup Kit
• Viral RNA/DNA Prep Kit • Plasmid Prep Kit • Gel Extraction Kit • Total RNA Prep Kit
Workflow
< Mini Prep > Magnetic
Bead
Lysis & protein precipitation Magnet Magnet
Cell harvest & remove cell debris DNA binding & washing DNA elution
< Midi, Maxi Prep > Cell debris DNA Binding Transfer
removal bead Magnetic bead eluted DNA
Cell harvest Lysis & protein precipitation DNA binding & washing DNA elution
& remove cell debris
< 96well Prep>
Cell harvest Lysis & protein precipitation DNA binding & washing DNA elution
& remove cell debris
126 BIOFACT CATALOGUE
DNA / RNA Preparation
QC DATA
< Plasmid Mini Prep> < Plasmid Midi Prep> < Plasmid Maxi Prep>
BIOFACT Magnetic Bead
Column Magnetic Bead Column Magnetic Bead Mini Midi Maxi
1 2 3 4 5 1 2 3 1 2 1 2 1 2
M M M M M M
M : 1kb DNA Ladder 1 : BIOFACT Column Type 2 : BIOFACT M : 1kb DNA Ladder 1 : Company A M : 1kb DNA Ladder 1 : -EF 2 : +EF +EF : Endotoxin Free
3 : Company A 4 : Company B 5 : Company C 2 : Company B 3 : BIOFACT
< PCR Purification using DaBead™ Magnetic Separation Stand (For 96 well plate & 8-strip tube)>
M M
M : 1kb DNA Ladder Before purification M : 1kb DNA Ladder After purification
Product Information
DaBead™ Magnetic Separation Stand
Magnetic Bead Binding
[SJ1-MSS96] [SJ1-MSS11]
(96well, 8-strip tube) (1.5 ㎖ × 12 hole, 50 ㎖ × 2 hole)
<Patent registration number : <Patent registration number :
No.10-1942097> No.10-1905136>
[SJ1-MSS03]
(1.5 ㎖ / 2.0 ㎖ × 2 hole)
Maker Cat.No. Product Size
SJ1-MSS11 DaBead™ Magnetic Separation Stand [Ver.2.0.] , 1.5㎖ × 12hole, 50㎖ × 2hole EA
BIOFACT SJ1-MSS03 DaBead™ Magnetic Separation Stand, 1.5㎖ / 2.0㎖ × 2 hole EA
SJ1-MSS96 DaBead™ Magnetic Separation Stand(96 well. 8-strip tube) EA
127
BIOFACT CATALOGUE
Patent registration number : No. 10-2011496
DaBead™ 96well / 48well
Magnetic Bead Press Pipettor
100X Cheaper & 6X Faster than Automated Extraction Machine
DaBead™ 96well / 48well Magnetic Press Pipettor reduces time and increases e ciency by purifying a large number of samples
(up to 96ea) at once. It is a magnetic accessary for Magnetic Bead binding.
Feature
• Two types (96well & 48 well) • Easy & convenience use
• No electricity required • Pre-filled prep kit available
• Compact size (Available on clean bench) : 177 x 256 x 355 (L x D x H, mm)
Workflow
- DaBead™ 96well / 48well Magnetic Bead Press Pipettor [SJ1-MPS96 / SJ1-MPS48]
Cell Lysis &
precipitation DNA binding Washing DNA elution
Related Product
• Genomic DNA Prep Kit
5. Recessed
• Viral RNA/DNA Prep Kit handle
1. Tip removal button
• PCR Purification Kit
2. Pin (Fixing pin)
Parts & Features
Parts Features
1 Tip removal button Detaching adaptor (8 x 12 / 8 x 6) tip from magnetic rod
A pin that holds the Swing lever (Bar) from going up when
2 Pin (Fixing pin) 6. Swing lever
collecting magnetic beads
(Bar)
Magnetic beads binding at the bottom with Adaptor 3. Nd
3 Nd Magnetic rod
(8 x 12 / 8 x 6) Tip Magnetic rod
4 Deepwell plate cradle Placing deepwell plate (Standard typed) for extraction
4. Deepwell
5 Recessed handle Lifting or moving the Press Pipettor plate cradle
Taking Nd Magnetic rod down by turning Swing lever (Bar)
[Counterclockwise]
6 Swing lever (Bar)
Raising Nd Magnetic rod up by turning Swing lever (Bar)
[Clockwise]
128 BIOFACT CATALOGUE
DNA / RNA Preparation
How to use
Attaching Adaptor Tip 1 Attaching Adaptor Tip 2 Detaching Adaptor Tip
①
②
When placing the plate on the cradle, check the direction of the plate and push it to the end. Hold the shell frame grid and raise it all toward Nd While pressing the ‘Tip removal button’, hold
magnetic rod until it clicks. the shell frame grid and drop it slowly to
separate the plate.
Fixing Swing lever (Bar) Tapping Dry For 48well Press Pipettor
Fixing Swing lever
(Bar)
After xing When Swing lever (Bar) is lowered When placing the plate on the Next, push the plate to the
and the pin is xed, Nd magnetic rod cradle, check the direction of the end of left side for use.
is xed in the downward position. plate and push it to the end of
right side for use.
QC DATA
< Extraction efficiency test using DaBead™ Magnetic Bead Press Pipettor – Plant >
M M
M M
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
Product Information
DaBead™ 96well Magnetic Bead DaBead™ 48well Magnetic Bead
Press Pipettor [SJ1-MPS96] Press Pipettor [SJ1-MPS48]
Maker Cat.No. Product Size
SJ1-MPS96 DaBead™ 96well Magnetic Press Pipettor EA
SJ1-MPS48 DaBead™ 48well Magnetic Press Pipettor EA
BIOFACT A96T-005 Adaptor (8 x 12) Tip [Adaptor 8-strip Tip (12 ea) + shell frame grid (1 ea) + 2 ㎖ Deep Well Plate] 5 rack
A48TX2-005 Adaptor (8 x 6) Tip [Adaptor 8-strip Tip (12 ea) + shell frame grid (2 ea) + 2 ㎖ Deep Well Plate] 5 rack
DP-221h Hisol™ 96 Square Deep Well Plate -2.2 ㎖ (prole concave) 100 ea / box
129
BIOFACT CATALOGUE
DaBead™ 8well
Magnetic Bead Pipettor
Extraction of Small Number of Samples (Up to 8ea) in a Short Time through Simple Process
DaBead™ 8well Magnetic Bead Pipettor reduces time and increases e ciency by purifying a small number of samples (up to 8ea)
through simple process. It is a magnetic accessary for magnetic bead binding.
Feature
• Compact size • Grid printed for easy identification
• Easy to mount & remove • Pre-filled cartridge for the pipettor
• Up to 8 samples extraction at once • Attachable in a magnet place
(Over 8 samples when used in combination)
- Up to & over 8 samples at once - Grid printed - Attachable in a magnet place - Easy mount & removal
- Compact size - Light weight - Pre-filled cartridge
Workflow
- DaBead™ Genomic DNA Prep Kit For Bacterium [GD801S-096]
Cell lysis &
precipitation DNA binding Washing DNA elution
Related Product
• Genomic DNA Prep Kit • Viral RNA/DNA Prep Kit • PCR Purification Kit • Total RNA Prep Kit
130 BIOFACT CATALOGUE
DNA / RNA Preparation
QC DATA
<Extraction efficiency test using DaBead™ 8well Magnetic Bead Pipettor – Plant>
M
M : 1kb DNA Ladder 1% Agarose gel 3 ㎕ loading Elution volume : 100 ㎕
Product Information
DaBead™ 8well Magnetic Bead Pipettor [SJ1-MBP08]
Maker Cat.No. Product Size
SJ1-MBP08 DaBead™ 8well Magnetic Bead Pipettor EA
BIOFACT
A8T-120 Adaptor 8-strip Tip 120 ea / box
131
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
Magnetic DaBead™ Easy
Column
Solution
Bead Type Type Type Type
Viral RNA/DNA Prep Kit
Ensuring High Quality Viral RNA/DNA using Magnetic Bead System
DaBead™ Viral RNA/DNA Prep Kit provides fast, simple and automated extraction of viral RNA/DNA from various samples such as
serum, plasma, cell-culture media, cell free body uids and tissue using magnetic bead system. This kit allows user to extract viral
RNA/DNA such as SARS-COV-2, Adenovirus, Norovirus, Zika virus, HCV and HIV using Magnetic Bead. Its magnetic bead is a sili-
ca-coated magnetic particle which is suitable for puri cation of nucleic acids due to its superior binding and dissociation features
through magnetic property. Viral RNA/DNA can be easily extracted using various instruments made by BIOFACT or a third party
such as 1.5 ㎖ Magnetic Stand, 8well Magnetic Pipettor, automatic nucleic acid extraction machine and 96well / 48well Magnetic
Press Pipettor.
Feature
• CE-IVD certified • No carrier RNA required
• No complex enzyme / instrument for extraction required • Safety use ; no phenol & no chloroform
• Available to use in hand & extraction machine
• In hand: 8well Magnetic Bead pipettor & 1.5 ㎖ Magnetic Separation Stand, 96well / 48well Magnetic Press Pipettor
• Extraction machine: KingFisher, TAN bead, MagNA Pure System, ZiXpress and the like.
Workflow
- DaBead™ Viral RNA/DNA Prep Kit _ 1.5 ㎖ Magnetic Stand [VN701-100]
Magnet
Cell Lysis & Magnetic Bead Magnetic Bead Viral RNA/DNA Magnet Eluted Viral RNA/DNA
precipitation biniding washing & Dry elution
- DaBead™ Viral RNA/DNA Prep Kit _ 8well Pipettor [VN801S-096]
Cell lysis &
precipitation DNA binding Washing DNA elution
132 BIOFACT CATALOGUE
DNA / RNA Preparation
- DaBead™ Viral RNA/DNA Prep Kit _ APX-32P [VN801A-096]
Sample Magnetic DNA
Bead
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Bead binding Lysis bu er Washing bu er1,2 Elution bu er Release bead
- DaBead™ Viral RNA/DNA Prep Kit _ 96well Press Pipettor [VN901-096, VN401-096]
Cell Lysis & DNA binding Washing DNA elution
precipitation
QC DATA
- Comparison of amplification efficiency in various primer sets between DaBead™ Viral RNA/DNA Prep Kit and
the other company’s viral RNA/DNA extraction kit
[Viral RNA Detection Primer Set-1] [Viral RNA Detection Primer Set-2]
FAM Texas Red FAM Texas Red
10 0 10 0
10 -1 10 -1
10 -2 10 -2
10 -3 10 -3
Column Type 8well Magnetic Bead Pipettor
Viral RNA 10 0 Viral RNA 10 -1 Viral RNA 10 -2 Viral RNA 10 -3
ΔCt value
FAM Texas Red FAM Texas Red FAM Texas Red FAM Texas Red
Primer Company A - Column Type 16.61 16.16 20.50 20.32 24.12 24.49 27.68 28.14
Set-1 8well Magnetic Bead Pipettor 16.14 16.43 19.60 20.03 23.07 23.83 26.77 27.98
Primer Company A - Column Type 16.11 14.94 19.87 18.59 23.88 22.63 26.94 25.65
Set-2 8well Magnetic Bead Pipettor 15.86 14.60 19.52 18.03 22.68 21.50 26.19 24.94
Comparison of the DaBead™ Viral RNA/DNA Prep Kit[Magnetic Bead Type], Campany A [Column Type] for extraction of novel coronavirus(COVID-19). COVID-19 viral RNA was purified from
10-fold diluted in spiked COVID-19 serum and detected by RT-PCR(Viral RNA Detection Primer Set-1/-2) using 1/10 volume of purified RNA solution. Measure from 1 X 10 6 copies using
COVID-19 Viral RNA.
[Viral RNA Detection Primer Set-3]
Viral RNA 10 0 Viral RNA 10 -1 Viral RNA 10 -2 Viral RNA 10 -3
Column Type Semi-Auto Magnetic Bead Type
Comparison of the DaBead™ Viral RNA/DNA Prep Kit[Magnetic Bead Type], HiGene™ Viral RNA/DNA Prep Kit [Column Type] for extraction of novel coronavirus(COVID-19).
COVID-19 viral RNA was purified from serially diluted in spiked COVID-19 serum and detected by RT-PCR(Viral RNA Detection Primer Set-3) using 1/10 volume of purified RNA solution.
Measure from 1 X 10 6 copies using COVID-19 Viral RNA.
133
BIOFACT CATALOGUE
QC DATA
- Comparison of amplification efficiency for extracted viral RNA/DNA in different batch products
Lot-1/ Lot-2 / Lot-3
FAM
1 X 10 7 copies 1 X 10 6 copies 1 X 10 5 copies 1 X 10 4 copies 1 X 10 3 copies 1 X 10 2 copies
using Viral RNA using Viral RNA using Viral RNA using Viral RNA using Viral RNA using Viral RNA
Texas Red
Copies No. Copies No.
Copies No. Copies No.
10 7 10 6 10 5 10 4 10 3 10 2 10 7 10 6 10 5 10 4 10 3 10 2
Lot-1 Average Ct value 22.82 26.46 30.06 33.81 36.20 39.18 Lot-1 Average Ct value 22.35 25.88 29.48 33.33 37.04 ND
[FAM] Lot-2 Average Ct value 22.84 26.54 30.15 34.00 36.94 39.65 [Texas Lot-2 Average Ct value 22.02 25.59 29.18 32.97 36.85 ND
Red]
Lot-3 Average Ct value 22.57 26.17 30.00 33.22 36.54 39.37 Lot-3 Average Ct value 21.95 25.52 29.22 32.83 36.50 ND
Standard Deviation 0.153 0.195 0.078 0.404 0.37 0.236 Standard Deviation 0.217 0.196 0.27 0.321 0.797 ND
%CV between Lot 0.67 0.74 0.26 1.20 1.01 0.60 %CV between Lot 0.98 0.76 0.92 0.97 2.16 ND
Lot-to-lot variation from DaBead™ Viral RNA/DNA Prep Kit[Cat. No. VN901-096, BioFACT] . COVID-19 viral RNA was purified from 10-fold diluted in spiked COVID-19 serum
and detected by qRT-PCR (Viral RNA Detection Primer Set-1) using 1/10 volume of purified RNA solution.
- Amplification test of extracted viral RNA/DNA from clinical samples using viral RNA detection primer set-1
(VN901-096, DaBead™ Viral RNA/DNA Prep Kit & SJ1-MPS96, DaBead™ 96well Magnetic Press Pipettor)
Negative Sample
Positive Sample
[FAM] [Texas Red] Internal control
134 BIOFACT CATALOGUE
DNA / RNA Preparation
Product Information
DaBead™ Viral RNA/DNA Prep Kit _ DaBead™ Viral RNA/DNA Prep Kit _
1.5 ㎖ Magnetic Stand [VN701-100] 8well Pipettor [VN801S-096]
▶Contents
VL ▶Contents
VB Reagent Plate
VW-1 Proteinase K
VW-2 (empty bottle) Adaptor 8-strip Tip
Magnetic Bead
RNase free water
Proteinase K
DaBead™ Viral RNA/DNA Prep Kit _ DaBead™ Viral RNA/DNA Prep Kit _
APX-32P [VN801A-096] 96well Press Pipettor [VN901-096]
▶Contents
VRL Plate
▶Contents Magnetic Bead Plate
Reagent Plate VW-1 Plate
Proteinase K VW-2 Plate
Adaptor 8-strip Tip
Elution Bu er Plate
Proteinase K
Adaptor(8 x 12) Tip
DaBead™ Viral RNA/DNA Prep Kit _
48well Press Pipettor [VN401-096]
▶Contents
VRL Plate + Magnetic Bead Plate
VW-1 + VW-2 Plate
Elution Bu er Plate
Proteinase K
Adaptor(8 x 6) Tip
Maker Cat.No. Product Size
VN701-100 DaBead™ Viral RNA/DNA Prep Kit _ 1.5 ㎖ Magnetic Stand 100 prep
VN801S-096 DaBead™ Viral RNA/DNA Prep Kit _ 8well Pipettor 16 prep x 6 plate
VN801A-096 DaBead™ Viral RNA/DNA Prep Kit _ APX-32P 16 prep x 6 plate
VN901-096 DaBead™ Viral RNA/DNA Prep Kit _ 96well Press Pipettor 96 prep x 1 plate
BIOFACT
VN401-096 DaBead™ Viral RNA/DNA Prep Kit _ 48well Press Pipettor 48 prep x 2 plate
A96T-005 Adaptor (8 X 12) Tip [Adaptor 8-strip Tip (12 ea) +shell frame grid (1 ea) + 2 ㎖ Deep Well Plate] 5 rack
A48TX2-005 Adaptor (8 X 6) Tip [Adaptor 8-strip Tip (12 ea) +shell frame grid (2 ea) + 2 ㎖ Deep Well Plate] 5 rack
DP-221h Hisol™ 96 Square Deep Well Plate -2.2 ㎖ (prole concave) 100 ea / box
* Plasticwares : VN701-100 (Not included), Rest of them (Included)
135
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
HiGene™
Type Viral RNA/DNA Prep Kit
Magnetic Solution Column Easy
Bead Type Type Type
High-Purity Viral RNA/DNA Extraction
HiGene™ Viral RNA/DNA Prep Kit[Column Type] can quickly and conveniently extract RNA from Serum, cell-culture media, cell free
uids sample or plasma. The process does not require the use of organic solvents or ethanol precipitation steps, and is thus ideal
for the safe and convenient extraction. This kit can be used for isolation of vial RNA/DNA from broad of RNA and DNA viruses such
as SARS-COV-2, Adenovirus, Norovirus, Zika virus, HCV and HIV. Procedural directions of HiGene™ Viral RNA/DNA Prep Kit[Column
Type] is very simple; users may purity Viral RNA/DNA from a variety of target sources within 20 minutes.
Feature
• CE-IVD certified • Efficient removal of impurities using non-alcoholic washing buffer
• No carrier RNA required • Glass microfiber membrane column based
• High purity & high yield • Safety use; no phenol & no chloroform
Application
• Quantitative PCR(qPCR, qRT-PCR) • cDNA synthesis
• Pathogen detection • Microarray
• Poly-A selection • Northern blotting
Workflow
- HiGene™ Viral RNA/DNA Prep Kit [VN101-100]
Column binding & Viral RNA/DNA Eluted Viral
Cell lysis
washing elution RNA/DNA
QC DATA
- Comparison of amplification efficiency for extracted viral RNA/DNA using HiGene™ Viral RNA/DNA Prep Kit and the other company’s
viral RNA/DNA extraction kit (qRT-PCR/qPCR)
<Adenovirus (DNA)> <Norovirus (RNA)>
BIOFACT Company Company
∆Ct value
[VN101] A B
Adenovirus 29.2 29.5 29.8
Norovirus 34.8 34.7 35.0
BIOFACT
Company A
Company B
136 BIOFACT CATALOGUE
DNA / RNA Preparation
<Hepacivirus C Virus (HCV)> <Dengue Virus (DV)>
BIOFACT Company Company
∆Ct value
[VN101] A B
HCV 31.1 35.2 32.9
DV 30.9 33.8 31.2
<Measles Virus (MV)> <Zika Virus (ZV)> MV 26.0 ND 27.2
ZV 28.0 29.7 27.8
BIOFACT
Company A
Company B
Product Information
HiGene™ Viral RNA/DNA Prep Kit [VN101-100]
▶Contents
VL
VW-1
VW-2 (empty bottle)
RNase free water
Proteinase K
Capsule Column
Maker Cat.No. Product Size
BIOFACT VN101-100 HiGene™ Viral RNA/DNA prep kit 100 prep
137
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
DaBead™
Magnetic Solution Column Easy
Bead Type Type Type Type
Genomic DNA Prep Kit
High-E ciency Genomic DNA Extraction using Magnetic Bead
DaBead™ Genomic DNA Prep Kit is a fast and easy way to extract genomic DNA from various samples such as bacterium, cultured
cell, whole Blood, animal Tissue, saliva, oral epithelial cell, hair root, plasma/serum, etc. using DaBead™ Magnetic Bead. DaBead™
Magnetic bead is designed for nucleic acid puri cation by coating the surface of Super Paramagnetic Nanoparticle with silica. As
the extraction process are similar to the column typed method, it is easy to use even without prior experience, and it can be used
conveniently by minimizing the centrifugation step. DaBead™ Genomic DNA Prep Kit can be used with DaBead™ Magnetic Separa-
tion Stand, DaBead™ 8well Magnetic Bead Pipettor, DaBead™ 96well / 48well Magnetic Bead Press Pipettor and various automatic
nucleic acid extraction equipment.
Feature
• Magnetic Bead type • Extraction from various types of samples
• Extraction with a small amount of sample available • Scale up extraction available (DaBead™ Magnetic Separation Stand, 1.5 ㎖ type)
• Quick process by minimizing the use of centrifuge • Avoiding cross-contamination between samples by using the single-use
• Easy to use even without prior experience consumables included in the kit (GD801S-096, GD901-096 & GD401-096)
• Safe use through no organic solvents used method
Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR etc.) • Construction of clone library
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP etc.)
Workflow
- DaBead™ Genomic DNA Prep Kit For Bacterium _ 1.5 ㎖ Magnetic Stand [GD701-100]
Magnet
Cell Lysis & Magnetic Bead Magnetic Bead Magnet
precipitation biniding washing & dry DNA elution Eluted DNA
- DaBead™ Genomic DNA Prep Kit For Bacterium _ 8well Pipettor [GD801S-096]
Cell lysis &
precipitation DNA binding Washing DNA elution
138 BIOFACT CATALOGUE
DNA / RNA Preparation
- DaBead™ Genomic DNA Prep Kit For Bacterium _ APX-32P [GD801A-096]
Sample Magnetic DNA
Bead
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Bead binding Lysis bu er Washing bu er1,2 Elution bu er Release bead
- DaBead™ Genomic DNA Prep Kit For Bacterium _ 96well Press Pipettor [GD901-096, GD401-096]
Cell Lysis & DNA binding Washing DNA elution
precipitation
QC DATA
- Comparison of extraction efficiency between DaBead™ Genomic DNA Prep Kit and other companies’ prep kits _ Bacterium,
Cultured cell
<Microorganism : Gram- (E.coli)> <Microorganism : Gram+ (Bacillus)> <Human : HEK293 Cultured Cell>
Column Magnetic Bead Column Magnetic Bead Column Magnetic Bead
M
M
M
M 1 2 3 1 4 5 M M 1 2 3 1 4 5 M M 1 2 3 1 4 5 M
M : 1kb DNA Ladder 1 : BIOFACT 2 : Company A 3 : Company B M : 1kb DNA Ladder 1 : BIOFACT 2 : Company A 3 : Company B M : 1kb DNA Ladder 1 : BIOFACT 2 : Company A 3 : Company B
4 : Company C 5 : Company D 4 : Company C 5 : Company D 4 : Company C 5 : Company D
- Comparison of extraction efficiency between DaBead™ Genomic DNA Prep Kit and other companies’ prep kits _ Whole Blood
<200 ㎕>
Column Magnetic Bead
M 1 2 3 1 4 5 M
M
M : 1kb DNA Ladder
1 : BIOFACT
2 : Company A
3 : Company B
4 : Company C
5 : Company D
- Comparison of extraction efficiency between DaBead™ Genomic DNA Prep Kit and other companies’ prep kits _ Animal Tissue
<Pork - 10 mg> <Pork - 40 mg>
Column Magnetic Bead Column Magnetic Bead
M
M 1 2 3 1 4 5 M M 1 2 3 1 4 5 M
M
M : 1kb DNA Ladder 1 : BIOFACT 2 : Company A 3 : Company B M : 1kb DNA Ladder 1 : BIOFACT 2 : Company A 3 : Company B
4 : Company C 5 : Company D 4 : Company C 5 : Company D
139
BIOFACT CATALOGUE
- Comparison of extraction efficiency on various storage periods _ Saliva, Oral Epithelial Cell
<Saliva - 500 ㎕>
M 1 2 3 4 M
M : 1kb DNA Ladder
1: Day 1 (Refrigerated)
2: Day 2 (Refrigerated)
3: Day 3 (Refrigerated)
4: Day 7 (Refrigerated)
※ Concentration decreases rapidly over time due to the death of bacteria cells.
- Genomic DNA extraction using DaBead™ Magnetic Separation Stand (96 well. 8-strip tube) and DaBead™ 8well Magnetic Bead Pipettor
< Whole Blood - 200 ㎕ > < Gram(-) Becterium - Cultured Cell 1㎖ > < Oral Epithelial Cell >
M M M
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
- Comparison of efficiency by extraction Method between BIOFACT Magnetic Bead type & SDS manual type _ Saliva
BIOFACT Magnetic Bead type M SDS manual type BIOFACT Magnetic Bead type M SDS manual type
M M
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
- Comparison of extraction efficiency between DaBead™ 8well Magnetic Bead Pipettor and automated nucleic acid preparation system
<Gram(-) Bacterium> <Gram(+) Bacterium> <Whole Blood>
Semi-Auto M Auto Semi-Auto M Auto Semi-Auto M Auto
10 8 cells 10 8 cells 200 ㎕
<Beef> <Oral Epithelial>
Semi-Auto M Auto Semi-Auto M Auto
Semi-Auto : 8well Magnetic Bead Pipettor
Auto : Automated Nucleic Acid Preparation System
M : 1kb DNA Ladder
25 mg 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
- Extraction efficiency test - DaBead™ 96well Magnetic Bead Press Pipettor
< Bacterium > < Bacterium >
M M
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading
Elution volume : 100 ㎕
140 BIOFACT CATALOGUE
DNA / RNA Preparation
Product Information
DaBead™ Genomic DNA Prep Kit For Bacterium, DaBead™ Genomic DNA prep kit For Animal Tissue
Cultured cell _ 1.5 ㎖ Magnetic stand [GD701-100] A_type, B_type [GD704-100A, GD704-100B]
▶Contents ▶Contents
MC1(-) Lysozme MAT1 RNase A
MC1(+) RNase A MAT2 Proteinase K
MC2 Proteinase K MW1 Magnetic Bead
MW1 Magnetic Bead MW2(empty bottle)
MW2(empty bottle) MW1 Additive
MW1 Additive ME
ME
DaBead™ Genomic DNA prep kit For Whole Blood DaBead™ Genomic DNA prep kit For Saliva,
[GD702-100] Oral Epithelial cell, Hair Root [GD705-100]
▶Contents ▶Contents
MB ME MS ME
MBW Proteinase K Lysis Bu er RNase A
MW1 Magnetic Bead Proteinase K Magnetic Bead
MW2(empty bottle) Washing Bu er 1
MW1 Additive Washing Bu er 2
Washing Bu er 3(empty bottle)
DaBead™ Genomic DNA Prep Kit For Bacterium, DaBead™ Genomic DNA Prep Kit For Bacterium,
Cultured cell _ 8well Pipettor [GD801S-096] Cultured cell_ 96well Press Pipettor [GD901-096]
_ APX-32P [GD801A-096]
▶Contents
MC1(+) Elution Buffer Plate
▶Contents MC1(-) RNase A
MC1(-) RNase A MC2 Proteinase K
MC1(+) Proteinase K Binding Buffer Plate Lysozyme
MC2 Plate(6ea) Magnetic Bead Plate Adaptor (8 x 12) Tip
Adaptor 8-Strip Tip Washing Buffer 1 Plate
Lysozyme Washing Buffer 2 Plate
Washing Buffer 3 Plate
DaBead™ Genomic DNA Prep Kit For Bacterium, Cultured cell
_ 48well Press Pipettor [GD401-096]
▶Contents
MC1(+) RNase A
MC1(-) Proteinase K
MC2 Lysozyme
Binding Bu er + Magnetic Bead Plate
Washing Bu er 1 + Washing Bu er 2 Plate
Washing Bu er 3 + Elution Bu er Plate
Adaptor (8 x 6) Tip
141
BIOFACT CATALOGUE
Maker Cat.No. Product Size
GD701-100 DaBead™ Genomic DNA Prep Kit For Bacterium, Cultured cell _ 1.5 ㎖ Magnetic stand 100 prep
GD801S-096 DaBead™ Genomic DNA Prep Kit For Bacterium, Cultured cell _ 8well Pipettor 16 prep x 6 plate
GD801A-096 DaBead™ Genomic DNA Prep Kit For Bacterium, Cultured cell _ APX-32P 16 prep x 6 plate
GD901-096 DaBead™ Genomic DNA Prep Kit For Bacterium, Cultured cell _ 96well Press Pipettor 96 prep x 1 Plate
GD401-096 DaBead™ Genomic DNA Prep Kit For Bacterium, Cultured cell _ 48well Press Pipettor 48 prep x 2 plate
GD702-100 DaBead™ Genomic DNA prep kit For Whole Blood _ 1.5 ㎖ Magnetic stand 100 prep
GD802S-096 DaBead™ Genomic DNA prep kit For Whole Blood _ 8well Pipettor 16 prep x 6 plate
GD802A-096 DaBead™ Genomic DNA prep kit For Whole Blood _ APX-32P 16 prep x 6 plate
GD902-096 DaBead™ Genomic DNA prep kit For Whole Blood _ 96well Press Pipettor 96 prep x 1 Plate
GD402-096 DaBead™ Genomic DNA prep kit For Whole Blood _ 48well Press Pipettor 48 prep x 2 plate
GD704-100Ⓐ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓐ_type _ 1.5 ㎖ Magnetic stand 100 prep
GD804S-096Ⓐ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓐ_type _ 8well Pipettor 16 prep x 6 plate
BIOFACT GD804A-096Ⓐ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓐ_type _ APX-32P 16 prep x 6 plate
GD904-096Ⓐ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓐ_type _ 96well Press Pipettor 96 prep x 1 Plate
GD404-096Ⓐ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓐ_type _ 48well Press Pipettor 48 prep x 2 plate
GD704-100Ⓑ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓑ_type _ 1.5 ㎖ Magnetic stand 100 prep
GD804S-096Ⓑ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓑ_type _ 8well Pipettor 16 prep x 6 plate
GD804A-096Ⓑ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓑ_type _ APX-32P 16 prep x 6 plate
GD904-096Ⓑ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓑ_type _ 96well Press Pipettor 96 prep x 1 Plate
GD404-096Ⓑ DaBead™ Genomic DNA prep kit For Animal Tissue Ⓑ_type _ 48well Press Pipettor 48 prep x 2 plate
GD705-100 DaBead™ Genomic DNA prep kit For Saliva, Oral Epithelial Cell, Hair Root _ 1.5 ㎖ Magnetic stand 100 prep
GD805S-096 DaBead™ Genomic DNA prep kit For Saliva, Oral Epithelial Cell, Hair Root _ 8well Pipettor 16 prep x 6 plate
GD805A-096 DaBead™ Genomic DNA prep kit For Saliva, Oral Epithelial Cell, Hair Root _ APX-32P 16 prep x 6 plate
GD905-096 DaBead™ Genomic DNA prep kit For Saliva, Oral Epithelial Cell, Hair Root _ 96well Press Pipettor 96 prep x 1 Plate
GD405-096 DaBead™ Genomic DNA prep kit For Saliva, Oral Epithelial Cell, Hair Root _ 48well Press Pipettor 48 prep x 2 plate
* Magnetic Bead can be purchased separately.
* Plasticwares : GD701-100, GD702-100, GD704-100Ⓐ, GD704-100Ⓑ, GD705-100 (Not included), Rest of them (Included)
142 BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type DNA / RNA Preparation
Easy Type
DaBead™ Genomic DNA
Magnetic Solution Column Easy
Bead Type Type Type Type
Prep Kit For Plant
High-E ciency Genomic DNA Extraction using Magnetic Bead
DaBead™ Genomic DNA Prep Kit allows you to quickly and easily extract genomic DNA from various plant samples using DaBead™
Magnetic Bead. DaBead™ Magnetic bead is designed for nucleic acid puri cation by coating the surface of Super Paramagnetic
Nanoparticle with silica. As the extraction process are similar to the column typed method, it is easy to use even without prior ex-
perience, and it can be used conveniently by minimizing the centrifugation step. DaBead™ Genomic DNA Prep Kit for Plant can be
used with DaBead™ Magnetic Separation Stand, DaBead™ 8well Magnetic Bead Pipettor, DaBead™ 96well / 48well Magnetic Bead
Press Pipettor and various automatic nucleic acid extraction equipment.
Feature
• Magnetic Bead type • Extraction from various types of plant samples
• Extraction with a small amount of sample available • Scale up extraction available (DaBead™ Magnetic Separation Stand, 1.5 ㎖ type)
• Quick process by minimizing the use of centrifuge • Avoiding cross-contamination between samples by using the single-use
• Easy to use even without prior experience consumables included in the kit (GD801S-096, GD901-096 & GD401-096)
• Safe use through no organic solvents used method
Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR etc.) • Construction of clone library
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP etc.)
Workflow
- DaBead™ Genomic DNA Prep Kit For Plant _ 1.5 ㎖ Magnetic Stand [GD703-100]
Magnet
Cell Lysis & Magnetic Bead Magnetic Bead DNA elution Magnet Eluted DNA
precipitation biniding washing & dry
- DaBead™ Genomic DNA Prep Kit For Plant _ 8well Pipettor [GD803S-096]
Cell lysis &
precipitation DNA binding Washing DNA elution
143
BIOFACT CATALOGUE
- DaBead™ Genomic DNA Prep Kit For Plant _ APX-32P [GD803A-096]
Sample Magnetic DNA
Bead
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Bead binding Lysis bu er Washing bu er1,2 Elution bu er Release bead
- DaBead™ Genomic DNA Prep Kit For Plant _ 96well Press Pipettor [GD903-096, GD403-096]
Cell Lysis & DNA binding Washing DNA elution
precipitation
QC DATA
- Comparison of efficiency by extraction method between BIOFACT Magnetic Bead type and column type
<Option>
Spinacia oleracea Oenanthe javanica Aralia cordata Capsella bursa-pastoris Castanea crenata Protocol Option :
(Leaf) (Stem) (Stem) (Root) (Leaf) Increase of yield when
treated with chloroform
Magnetic Magnetic Magnetic Magnetic
M Column Bead M Column Bead M Column Bead M Column Bead M 1 2 3
M : 1kb DNALadder 1: BIOFACT Column Type 2 : BIOFACT Magnetic Bead Type M : 1kb DNALadder 1: BIOFACT Column Type
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕ 2 : BIOFACT Magnetic Bead Type (Chloroform(-))
3 : BIOFACT Magnetic Bead Type (Chloroform(+))
- Comparison of template LOD
Spinacia oleracea Oenanthe javanica Aralia cordata Capsella bursa-pastoris
(Leaf) (Stem) (Stem) (Root)
Spinacia oleracea Oenanthe javanica Aralia cordata Capsella bursa-pastoris
M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 (Leaf) (Stem) (Stem) (Root)
M : 1kb DNA Ladder 1 : Undiluted (5 0 ) 2 : 5 -1 3 : 5 -2 4 : 5 -3 Target Gene : rpoB Template : Undiluted (5 0 ) , 5 -1 , 5 -2 , 5 -3 Target Gene : 18S rRNA
** As a result of the Template LOD test, PCR can be performed without ** As a result of the Template LOD test, PCR can be performed without
PCR Inhibition (High Purity) PCR Inhibition (High Purity)
144 BIOFACT CATALOGUE
DNA / RNA Preparation
- Genomic DNA extraction using DaBead™ Magnetic Separation Stand (96 well. 8-strip tube) and DaBead™ 8well Magnetic Bead Pipettor
< Plant - 40mg >
M
M : 1kb DNA Ladder
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
- Comparison of extraction efficiency on various seed types between BIOFACT Magnetic Bead type and column type
Perilla Lactuca Daucus Raphanus Raphanus Brassica
frutescens sativa carota sativus L. sativus L. rapa L.
M A B A B A B A B A B A B M
M : 1kb DNA Ladder
A : Column
B : Magnetic Bead
- Comparison of extraction efficiency between DaBead™ 8well Magnetic Bead Pipettor and automated nucleic acid preparation system
<Plant Leaf> <Plant Stem>
Semi-Auto Auto Semi-Auto Auto
M M
40 mg 40 mg
<Plant Root> <Seed>
Semi-Auto Auto Semi-Auto Auto
M M
Semi-Auto : 8well Magnetic Bead Pipettor
Auto : Automated Nucleic Acid Preparation System
M : 1kb DNA Ladder
40 mg 50 mg 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
- Extraction efficiency test - DaBead™ 96well Magnetic Bead Press Pipettor
< Plant - Abeliophyllum distichum, Leaf> < Plant - Oryza sativa, Leaf >
M M
M M
M : 1kb DNA Ladder
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
145
BIOFACT CATALOGUE
Product Information
DaBead™ Genomic DNA Prep Kit For Plant A type DaBead™ Genomic DNA Prep Kit For Plant C type
_1.5 ㎖ Magnetic Stand [GD703-100A] _1.5 ㎖ Magnetic Stand [GD703-100C]
▶Contents
Lysis Bu er
▶Contents Binding Bu er
Lysis Bu er Washing Bu er 1
Washing Bu er 1 Washing Bu er 2(empty bottle)
Washing Bu er 2 MW1 additive
Washing Bu er 3(empty bottle) Elution Bu er
Elution Bu er RNase A
Magnetic Bead Proteinase K
RNase A Magnetic Bead
DaBead™ Genomic DNA Prep Kit For Plant B type DaBead™ Genomic DNA Prep Kit For Plant D type
_1.5 ㎖ Magnetic Stand [GD703-100B] _1.5 ㎖ Magnetic Stand [GD703-100D]
▶Contents ▶Contents
Lysis Bu er Lysis Bu er
Binding Bu er Binding Bu er
Washing Bu er 1 Washing Bu er 1
Washing Bu er 2 Washing Bu er 2
Washing Bu er 3(empty bottle) Washing Bu er 3(empty bottle)
Elution Bu er Elution Bu er
RNase A RNase A
Proteinase K Proteinase K
Magnetic Bead Magnetic Bead
DaBead™ Genomic DNA Prep Kit For Plant E type DaBead™ Genomic DNA Prep Kit For Plant G type
_1.5 ㎖ Magnetic Stand [GD703-100E] _1.5 ㎖ Magnetic Stand [GD703-100G]
▶Contents ▶Contents
Lysis Bu er Lysis Bu er
Binding Bu er Binding Bu er
Washing Bu er 1 Washing Bu er 1
Washing Bu er 2 Washing Bu er 2
Washing Bu er 3(empty bottle) Washing Bu er 3(empty bottle)
Elution Bu er Elution Bu er
RNase A RNase A
Proteinase K Magnetic Bead
Magnetic Bead
DaBead™ Genomic DNA Prep Kit For Plant A type DaBead™ Genomic DNA Prep Kit For Plant A type
_8well Pipettor [GD803S-096A], _APX-32P [GD803A-096A] _96well Press Pipettor [GD903-096A]
▶Contents
Lysis Bu er
▶Contents Binding Bu er Plate
Magnetic Bead Plate
Lysis Bu er Washing Bu er 1 Plate
RNase A Washing Bu er 2 Plate
Plate(6ea) Washing Bu er 3 Plate
Adaptor 8-Strip Tip Elution Bu er Plate
RNase A
Adaptor (8 x 12) Tip
DaBead™ Genomic DNA Prep Kit For Plant A type
_48well Press Pipettor [GD403-096A]
▶Contents
Lysis Bu er
Binding Bu er + Magnetic Bead Plate
Washing Bu er 1 + Washing Bu er 2 Plate
Washing Bu er 3 + Elution Bu er Plate
RNase A
Adaptor (8 x 6) Tip
146 BIOFACT CATALOGUE
DNA / RNA Preparation
Reference
Kyeong-Ryeol Lee, et al. Increasing Monounsaturated Fatty Acid Contents in Hexaploid Camelina sativa Seed Oil by FAD2 Gene Knockout Using CRISPR-Cas9. Front.
Plant Sci., 29 June 2021.
Maker Cat.No. Product Size
GD703-100Ⓐ DaBead™ Genomic DNA Prep Kit For Plant Ⓐ type_1.5 ㎖ Magnetic Stand 100 prep
GD803S-096Ⓐ DaBead™ Genomic DNA Prep Kit For Plant Ⓐ type_8well Pipettor 16 prep x 6 plate
GD803A-096Ⓐ DaBead™ Genomic DNA Prep Kit For Plant Ⓐ type_APX-32P 16 prep x 6 plate
GD903-096Ⓐ DaBead™ Genomic DNA Prep Kit For Plant Ⓐ type_96well Press Pipettor 96 prep x 1 Plate
GD403-096Ⓐ DaBead™ Genomic DNA Prep Kit For Plant Ⓐ type_48well Press Pipettor 48 prep x 2 plate
GD703-100Ⓑ DaBead™ Genomic DNA Prep Kit For Plant Ⓑ type_1.5 ㎖ Magnetic Stand 100 prep
GD803S-096Ⓑ DaBead™ Genomic DNA Prep Kit For Plant Ⓑ type_8well Pipettor 16 prep x 6 plate
GD803A-096Ⓑ DaBead™ Genomic DNA Prep Kit For Plant Ⓑ type_APX-32P 16 prep x 6 plate
GD903-096Ⓑ DaBead™ Genomic DNA Prep Kit For Plant Ⓑ type_96well Press Pipettor 96 prep x 1 Plate
GD403-096Ⓑ DaBead™ Genomic DNA Prep Kit For Plant Ⓑ type_48well Press Pipettor 48 prep x 2 plate
GD703-100Ⓒ DaBead™ Genomic DNA Prep Kit For Plant Ⓒ type_1.5 ㎖ Magnetic Stand 100 prep
GD803S-096Ⓒ DaBead™ Genomic DNA Prep Kit For Plant Ⓒ type_8well Pipettor 16 prep x 6 plate
GD803A-096Ⓒ DaBead™ Genomic DNA Prep Kit For Plant Ⓒ type_APX-32P 16 prep x 6 plate
GD903-096Ⓒ DaBead™ Genomic DNA Prep Kit For Plant Ⓒ type_96well Press Pipettor 96 prep x 1 Plate
GD403-096Ⓒ DaBead™ Genomic DNA Prep Kit For Plant Ⓒ type_48well Press Pipettor 48 prep x 2 plate
GD703-100Ⓓ DaBead™ Genomic DNA Prep Kit For Plant Ⓓ type_1.5 ㎖ Magnetic Stand 100 prep
GD803S-096Ⓓ DaBead™ Genomic DNA Prep Kit For Plant Ⓓ type_8well Pipettor 16 prep x 6 plate
BIOFACT GD803A-096Ⓓ DaBead™ Genomic DNA Prep Kit For Plant Ⓓ type_APX-32P 16 prep x 6 plate
GD903-096Ⓓ DaBead™ Genomic DNA Prep Kit For Plant Ⓓ type_96well Press Pipettor 96 prep x 1 Plate
GD403-096Ⓓ DaBead™ Genomic DNA Prep Kit For Plant Ⓓ type_48well Press Pipettor 48 prep x 2 plate
GD703-100Ⓔ DaBead™ Genomic DNA Prep Kit For Plant Ⓔ type_1.5 ㎖ Magnetic Stand 100 prep
GD803S-096Ⓔ DaBead™ Genomic DNA Prep Kit For Plant Ⓔ type_8well Pipettor 16 prep x 6 plate
GD803A-096Ⓔ DaBead™ Genomic DNA Prep Kit For Plant Ⓔ type_APX-32P 16 prep x 6 plate
GD903-096Ⓔ DaBead™ Genomic DNA Prep Kit For Plant Ⓔ type_96well Press Pipettor 96 prep x 1 Plate
GD403-096Ⓔ DaBead™ Genomic DNA Prep Kit For Plant Ⓔ type_48well Press Pipettor 48 prep x 2 plate
GD703-100Ⓕ DaBead™ Genomic DNA Prep Kit For Plant Ⓕ type_1.5 ㎖ Magnetic Stand 100 prep
GD803S-096Ⓕ DaBead™ Genomic DNA Prep Kit For Plant Ⓕ type_8well Pipettor 16 prep x 6 plate
GD803A-096Ⓕ DaBead™ Genomic DNA Prep Kit For Plant Ⓕ type_APX-32P 16 prep x 6 plate
GD903-096Ⓕ DaBead™ Genomic DNA Prep Kit For Plant Ⓕ type_96well Press Pipettor 96 prep x 1 Plate
GD403-096Ⓕ DaBead™ Genomic DNA Prep Kit For Plant Ⓕ type_48well Press Pipettor 48 prep x 2 plate
GD703-100Ⓖ DaBead™ Genomic DNA Prep Kit For Plant Ⓖ type_1.5 ㎖ Magnetic Stand 100 prep
GD803S-096Ⓖ DaBead™ Genomic DNA Prep Kit For Plant Ⓖ type_8well Pipettor 16 prep x 6 plate
GD803A-096Ⓖ DaBead™ Genomic DNA Prep Kit For Plant Ⓖ type_APX-32P 16 prep x 6 plate
GD903-096Ⓖ DaBead™ Genomic DNA Prep Kit For Plant Ⓖ type_96well Press Pipettor 96 prep x 1 Plate
GD403-096Ⓖ DaBead™ Genomic DNA Prep Kit For Plant Ⓖ type_48well Press Pipettor 48 prep x 2 plate
* Magnetic Bead can be purchased separately.
* Plasticwares : GD703-100Ⓐ, GD703-100Ⓑ, GD703-100Ⓒ, GD703-100Ⓓ, GD703-100Ⓔ, GD703-100Ⓕ, GD703-100Ⓖ (Not included), Rest of them (Included)
147
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
DaBead™ GMO DNA
Magnetic Solution Column Easy
Bead Type Type Type Type
Extraction Kit I For Potato, Bean, Maize
High-E ciency GMO DNA Extraction using Magnetic Bead
DaBead™ Genomic DNA Prep Kit is a kit for extracting GMO DNA using Magnetic Bead. It can extract DNA from various sample
samples (Potato, Bean, Maize), and it is easy to use even without prior experience as extraction process is simple and convenient.
Feature
• Magnetic Bead type • Extraction from GMO-modified plant samples
• Extraction with a small amount of sample available • Scale up extraction available
• Quick process by minimizing the use of centrifuge (DaBead™ Magnetic Separation Stand, 1.5 ㎖ type)
• Easy to use even without prior experience
Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR etc.) • Construction of clone library
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP etc.)
Workflow
- DaBead™ GMO DNA Extraction Kit [GD706-100]
Magnet
Cell Lysis & Magnetic Bead Magnetic Bead Magnet
precipitation biniding washing & dry DNA elution Eluted DNA
QC DATA
- Comparison of extraction efficiency on various scales between BIOFACT Magnetic Bead type and column type
<Potato> <Bean> <Maize>
0.1g 0.2g 0.3g 0.4g 0.5g 0.1g 0.2g 0.3g 0.4g 0.5g 0.1g 0.2g 0.3g 0.4g 0.5g
M A B A B A B A B A B M M A B A B A B A B A B M M A B A B A B A B A B M
M : 1kb DNA Ladder A : Column B : Magnetic Bead M : 1kb DNA Ladder A : Column B : Magnetic Bead M : 1kb DNA Ladder A : Column B : Magnetic Bead
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
148 BIOFACT CATALOGUE
DNA / RNA Preparation
- Comparison of PCR efficiency on various template concentration between BIOFACT Magnetic Bead type and column type
<Potato> <Bean> <Maize>
M Column M Magnetic Bead M Column M Magnetic Bead M Column M Magnetic Bead
* Primer mixture : rbcL(Plant - chloroplast DNA) * Primer mixture : rpoB(Plant - chloroplast DNA) * Primer mixture : ITS2(Plant - chloroplast DNA)
1 : Undiluted (5 0 ) 2 : 10 -1 3 : 10 -2 4 : 10 -3 5 : 10 -4 6 : 10 -5 1 : Undiluted (5 0 ) 2 : 10 -1 3 : 10 -2 4 : 10 -3 5 : 10 -4 6 : 10 -5 1 : Undiluted (5 0 ) 2 : 10 -1 3 : 10 -2 4 : 10 -3 5 : 10 -4 6 : 10 -5
- Comparison of extraction efficiency according to various sampling methods between BIOFACT Magnetic Bead type and
column type
<Potato - 50 mg> <Bean - 40 mg> <Maize - 100 mg>
Soy Bean Black Bean Red Bean
Liquid Liquid Liquid Liquid
Lump Piece Crushed Grinder nitrogen Grinder nitrogen Grinder nitrogen Grinder nitrogen Grinder Crushed
M A B A B A B A B M M A B A B A B A B A B A B M M A B A B A B M
M : 1kb DNA Ladder A : Column B : Magnetic Bead M : 1kb DNA Ladder A : Column B : Magnetic Bead M : 1kb DNA Ladder A : Column B : Magnetic Bead
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
- Comparison of extraction efficiency between BIOFACT Magnetic Bead type and automated nucleic acid preparation system
BIOFACT Magnetic Bead Auto BIOFACT Magnetic Bead Auto
1 2 3 4 5 6 M 1 2 3 4 5 6 13 14 15 16 17 18 M 13 14 15 16 17 18
BIOFACT Magnetic Bead Auto BIOFACT Magnetic Bead Auto
7 8 9 10 11 12 M 7 8 9 10 11 12 19 20 21 22 23 24 M 19 20 21 22 23 24
1 : GT73 rapeseed (GMO) 2 : NG rapeseed (NON-GMO) 3 : Carrot seed 4 : Tiny(tomato) 5 : Beotimmog 6 : Berryking 7 : Supersun
8 : Hoyong 9 : Kyupilang 10 : Lovely240 11 : Mailokku 12 : Redpam 13 : Sangcherry 14 : Betatine
15 : Lapito 16 : Shinnuri 17 : Hambak 18 : Shinlovely 19 : Rokkusan 20 : Minichal 21 : Shinshu
22 : Shinlovely 23 : Potato1 24 : Potato2 M : 1kb DNA Ladder
M : 1kb DNA Ladder
BIOFACT Magnetic Bead : 1.5 ㎖ Magnetic stand
Auto : Automated Nucleic Acid Preparation System
1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
149
BIOFACT CATALOGUE
Product Information
DaBead™ GMO DNA Extraction Kit For Potato, Bean, Maize_1.5 ㎖ Magnetic Stand 〔GD706-100〕
▶Contents
Lysis Bu er
Binding Bu er
Washing Bu er 1
Washing Bu er 2
Washing Bu er 3 (empty bottle)
Elution Bu er
RNase A
Proteinase K
Magnetic Bead
Maker Cat.No. Product Size
GD706-100 DaBead™ GMO DNA Extraction Kit For Potato, Bean, Maize_1.5 ㎖ Magnetic Stand 100 prep
GD806S-096 DaBead™ GMO DNA Extraction Kit For Potato, Bean, Maize_8well Pipettor 16 prep x 6 plate
BIOFACT GD806A-096 DaBead™ GMO DNA Extraction Kit For Potato, Bean, Maize_APX-32P 16 prep x 6 plate
GD906-096 DaBead™ GMO DNA Extraction Kit For Potato, Bean, Maize_96well Press Pipettor 96 prep x 1 plate
GD406-096 DaBead™ GMO DNA Extraction Kit For Potato, Bean, Maize_48well Press Pipettor 48 prep x 2 plate
* Magnetic Bead can be purchased separately.
* Plasticwares : GD706-100 (Not included), Rest of them (Included)
150 BIOFACT CATALOGUE
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