Column Type
Solution Type
Magnetic Bead Type DNA / RNA Preparation
Easy Type
Magnetic DaBead™ Genomic DNA
Column
Solution
Easy
Bead Type Type Type Type
Extraction Kit For Rice
High-E ciency Rice DNA Extraction using Magnetic Bead
DaBead™ Genomic DNA Extraction Kit for Rice is a kit for extracting genomic DNA using Magnetic Bead. It can extract genomic
DNA from various rice varieties and it is easy to use even without prior experience as extraction process is simple and convenient.
Feature
• Magnetic Bead type • Extraction from rice samples
• Extraction with a small amount of sample available • Easy to use even without prior experience
• Quick process by minimizing the use of centrifuge
Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR etc.) • Construction of clone library
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP etc.)
Workflow
- DaBead™ 96well / 48well Magnetic Bead Press Pipettor [SJ1-MPS96 / SJ1-MPS48]
Cell Lysis & DNA binding Washing DNA elution
precipitation
QC DATA
- Comparison of genomic DNA extraction from Rice Varieties using DaBead™ Genomic DNA Extraction Kit for Rice
< Genomic DNA extraction from 1 grain of rice >
M Cheongmu Woongwang Hopyeong M Daean Hwayoung Hwaseong
M Geumseong Boramchan Samcheon M Milkyqueen Dongjinilho Soby
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
151
BIOFACT CATALOGUE
- Comparison of PCR efficiency on various rice varieties using DaBead™ Genomic DNA Extraction Kit for Rice
Cheongmu Woongwang Hopyeong Daean Hwayoung Hwaseong
M M M M M M
Geumseong Boramchan Samcheon Milkyqueen Dongjinilho Soby
M M M M M M
M : DNA marker for rice varieties 3% Agarose gel 7㎕ loading
Product Information
DaBead™ Genomic DNA Extraction Kit For Rice [GD707-480]
▶Contents
Lysis Bu er Elution Bu er
Binding Bu er RNase A
Washing Bu er 1 Proteinase K
Washing Bu er 2 (empty bottle) Magnetic Bead
Maker Cat.No. Product Size
BIOFACT GD707-480 DaBead™ Genomic DNA Extraction Kit For Rice 480 prep
* Magnetic Bead can be purchased separately.
152 BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type DNA / RNA Preparation
Easy Type
DaBead™ Cell Free DNA
Magnetic Solution Column Easy
Bead Type Type Type Type
Prep Kit For Plasma, Serum
Cell Free DNA Extraction using Magnetic Bead
DaBead™ Cell Free DNA Prep Kit For Plasma, Serum is a kit for extracting Cell Free DNA using Magnetic Bead.
It can extract cell free DNA extraction from plasma and serum and it is easy to use even without prior experience as extraction
process is simple and convenient.
Feature
• Magnetic Bead type • Cell free DNA from plasma and serum
• Extraction with a small amount of sample available • Scale up extraction available
• Quick process by minimizing the use of centrifuge (DaBead™ Magnetic Separation Stand, 1.5 ㎖ type)
• Easy to use even without prior experience
Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR etc.) • Genotyping (SNP, SSR, AFLP, T-RFLP etc.)
Workflow
- DaBead™ Cell Free DNA Prep Kit [GD708-100]
Magnet
Cell Lysis & Magnetic Bead Magnetic Bead DNA elution Magnet Eluted cell Free DNA
precipitation biniding washing & dry
QC DATA
- qPCR test using cell free DNA from DaBead™ Cell Free DNA Prep Kit For Plasma, Serum and other company’s
cell free DNA prep kit
< Gene : ß-globlin >
> Sample : 2 0 > Sample : 2 -1
Company A Company A
BIOFACT BIOFACT
△Ct value 2 0 2 -1 NTC △Rn 2 0 2 -1
Company A 33.27 34.27 Company A 19.61 21.39
ND
BIOFACT 31.55 32.4 BIOFACT 20.07 19.08
153
BIOFACT CATALOGUE
< Gene : GAPDH>
> Sample : 2 0 > Sample : 2 -1
Company A Company A
BIOFACT BIOFACT
△Ct value 2 0 2 -1 NTC △Rn 2 0 2 -1
Company A 35.01 35.26 Company A 14.74 13.18
ND
BIOFACT 34.7 35.14 BIOFACT 16.61 15.56
Product Information
DaBead™ Cell Free DNA Prep Kit For Plasma, Serum [GD708-100]
▶Contents
Lysis Bu er
Washing Bu er 1
Washing Bu er 2
Washing Bu er 3 (empty bottle)
Elution Bu er
Proteinase K
Magnetic Bead
Maker Cat.No. Product Size
BIOFACT GD708-100 DaBead™ Cell Free DNA Prep Kit For Plasma, Serum 100 prep
* Magnetic Bead can be purchased separately.
154 BIOFACT CATALOGUE
Column Type DNA / RNA Preparation
Solution Type
Magnetic Bead Type
Easy Type
HiGene™
Genomic DNA Prep Kit
Magnetic Solution Column Easy
Bead Type Type Type Type
Isolation of Pure Genomic DNA from Various Samples
HiGene™ Genomic DNA Prep Kit [Column type] is designed to isolate the genomic DNA using glass micro ber membrane as a
spin column type. The kit provides pure and high quality genomic DNA preparation system by removing PCR inhibition sub-
stances such as divalent cations and proteins. HiGene™ Genomic DNA Prep Kit [Column type] is using convenient method for the
isolation of pure genomic DNA directly from various samples including blood, bacteria, plants and others.
Feature
• Spin column type • Column binding capacity : 20 ㎍ DNA / Column
• Membrane : Glass micro ber
Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR and etc.) • Clone library construction
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP, etc.)
Workflow
- HiGene™ Genomic DNA Prep Kit [GD141-100]
Mixing animal tissue Column binding &
+ GD1 + Proteinase K + GD2 + GB washing Elute DNA Pure genomic DNA
+ RNase A
Know-how for high yield
1. Please use recommended volume / amount of each sample. In case of isolation from large amount of sample,
adjust the input volume of each solution in proportion to the amount of sample.
2. Prepare 80% Ethanol (not provided) in a WB Bottle. Make it fresh before use.
3. Please remove EtOH completely thorough column centrifugation before using DNA Hydration Solution.
4. Add dried enzyme to D.W (Bu er) and mix well. Please store it at -20°C.
5. Please use the product according to its expiration date.
6. Please do not vortexing strongly to avoid DNA degradation during cell lysis step.
7. Storage of GD1 at lower temperature may cause precipitation of salts due to SDS.
In this case, please use it after thawing in the microwave/dry oven.
8. Elution e ciency can be increased when using DNA Hydration Solution after pre-heating at 50 °C for 10 minutes.
(Especially, in case of large DNA fragment)
155
BIOFACT CATALOGUE
QC DATA
- Comparison of isolation efficiency on various sample volume between HiGene™ Genomic DNA Prep Kit (Ver.2.0)
[Column Type] and other companies' prep kits (column type)
<Animal Tissue - Bovine Tissue>
BIOFACT Company A
10 mg 40 mg 80 mg 10 mg 40 mg 80 mg Conc. OD Total
M M Bovine Sample (ng/µl) (260/280) gDNA vol.
1 32.0
10 mg 1.98 3.2 ㎍
2 33.1
1 78.0
BIOFACT 40 mg 1.87 8.2 ㎍
2 86.5
1 132.0
80 mg 1.88 14.2 ㎍
2 152.5
1 41.3
10 mg 2.11 4.5 ㎍
2 49.2
1 72.4
Company A 40 mg 2.00 7.3 ㎍
2 74.8
Company B Company C 80 mg 1 126.9 2.02 12.8 ㎍
2 130.5
10 mg 40 mg 80 mg 10 mg 40 mg 80 mg 10 mg 1 31.5 2.09 3.0 ㎍
M M 2 30.8
1 64.3
Company B 40 mg 2.05 6.4 ㎍
2 64.9
1 84.6
80 mg 2.03 7.7 ㎍
2 71.4
1 26.5
10 mg 2.53 2.5 ㎍
2 25.4
1 68.6
Company C 40 mg 2.00 6.3 ㎍
2 59.3
1 61.2
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕ 80 mg 2 84.6 2.00 7.2 ㎍
<Plant - Lettus>
Total
Conc.
OD
BIOFACT Company A Company B Company C Bovine Sample Sample (ng/µl) (260/280) gDNA vol.
Vol
10 mg 40 mg 10 mg 40 mg 10 mg 40 mg 10 mg 40 mg 10 mg 1 13.0 1.98
M 2 13.3 1.3 ㎍
BIOFACT 1 45.2
40 mg 1.87 4.3 ㎍
2 41.8
1 11.4
10 mg 1.88 1.1 ㎍
Company A 2 1 11.0
33.4
40 mg 2.11 3.2 ㎍
2 32.6
1 11.3
10 mg 2.00 1.1 ㎍
2 11.5
Company B
40 mg 1 32.2 2.02 3.2 ㎍
2 32.4
1 13.1
10 mg 2.09 1.3 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕ Company C 2 1 13.0
29.4
40 mg 2.05 2.8 ㎍
2 28.0
156 BIOFACT CATALOGUE
DNA / RNA Preparation
- Comparison of gDNA isolation efficiency between HiGene™ Genomic DNA Prep Kit (Ver.2.0) [Column Type]
and other company's prep kits (column type)
<Microorganism - Gram- (E.coli)> OD 평균
E.coli Sample Conc. OD Total
Concentration
E.coli Sample Conc. (260/280) (260/280) Total 농도
[Average]
M BIOFACT Company A Company B Company C 1 1 58.7 ng/㎕
58.7 ng/µl
BIOFACT 2 56.5 ng/µl 2.00 6.0 ug
BIOFACT 2 56.5 ng/㎕ 2.00 6.0 ㎍
68.2 ng/µl
3 3 68.2 ng/㎕ OD 평균
1
20.0 ng/µl
E.coli 1 Sample 20.0 ng/㎕ Conc. (260/280) Total 농도
21.1 ng/µl
Company A A 사 2 2 21.1 ng/㎕ 2.28 2.28 2.1 ㎍ 2.1 ug
58.7 ng/µl
22.2 ng/µl
3 3 1 22.2 ng/㎕
BIOFACT 1 2 56.5 ng/µl 2.00 6.0 ug
57.4 ng/µl
1 57.4 ng/㎕
68.2 ng/µl
67.9 ng/µl
Company B B 사 2 2 3 67.9 ng/㎕ 2.10 2.10 6.2 ㎍ 6.2 ug
62.1 ng/µl
20.0 ng/µl
3 3 1 62.1 ng/㎕
49.0 ng/µl
21.1 ng/µl
A 사 1 1 2 49.0 ng/㎕ 2.28 2.1 ug
41.1 ng/µl
C 사 2 3 22.2 ng/µl 0.75 4.4 ug
Company C 2 41.1 ng/㎕ 0.75 OD 4.4 ㎍ 평균
Conc.
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading E.coli 3 Sample 44.2 ng/µl (260/280) Total 농도
57.4 ng/µl
3 1
44.2 ng/㎕
Elution volume : 100 ㎕ Cell number : 8 X 10 8 B 사 2 67.9 ng/µl 2.10 6.2 ug
1 3 58.7 ng/µl
62.1 ng/µl
BIOFACT 2 56.5 ng/µl 2.00 6.0 ug
1 49.0 ng/µl
C 사 3 2 68.2 ng/µl 0.75 4.4 ug
41.1 ng/µl
1 3 20.0 ng/µl
44.2 ng/µl
A 사 2 21.1 ng/µl 2.28 2.1 ug
OD
평균
Bacillus Sample 22.2 ng/µl (260/280) Total 농도
Conc.
3
1 57.4 ng/µl
<Microorganism - Gram+ (Bacillus)> B 사 1 2 29.6 ng/µl 2.10 6.2 ug
67.9 ng/µl
BIOFACT 2 3 28.9 ng/µl 2.15 2.9 ug
62.1 ng/µl
49.0 ng/µl
M BIOFACT Company A Company B M 3 1 29.6 ng/µl ODTotal 평균
26.8 ng/µl OD
Conc.
Concentration
4.4 ug
0.75
Bacillus Bacillus Sample Conc. 41.1 ng/µl (260/280) (260/280) Total 농도
1 2
C 사 Sample
[Average]
A 사 2 3 27.1 ng/µl 2.45 2.6 ug
44.2 ng/µl
1 3 1 29.6 ng/㎕
29.6 ng/µl
26.5 ng/µl
BIOFACT 1 2 28.9 ng/㎕ 2.15 2.9 ㎍ 2.9 ug
BIOFACT 2
29.5 ng/µl 2.15
28.9 ng/µl
29.6 ng/µl
28.8 ng/µl
B 사 3 2 3 29.6 ng/㎕ 2.45 2.8 ug
26.8 ng/µl
1 3 1 26.8 ng/㎕
27.9 ng/µl
Company A A 사 2 2 27.1 ng/㎕ 2.45 2.6 ㎍ 2.6 ug
27.1 ng/µl 2.45
26.5 ng/µl
3 3 26.5 ng/㎕ OD 평균
Bacillus 1 Sample 29.5 ng/µl (260/280) Total 농도
Conc.
1 29.5 ng/㎕
Company B B 사 2 2 28.8 ng/㎕ 2.45 2.8 ㎍ 2.8 ug
28.8 ng/µl 2.45
1
29.6 ng/µl
27.9 ng/µl
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading BIOFACT 3 3 27.9 ng/㎕ 2.15 2.9 ug
28.9 ng/µl
2
Elution volume : 100 ㎕ Cell number : 8 X 10 8 Blood Sample 29.6 ng/µl OD 평균
Conc.
3
1 1 26.8 ng/µl (260/280) Total 농도
37.1 ng/µl
A 사
BIOFACT 2 27.1 ng/µl 2.45 2.6 ug
1.92
3.9 ug
2 41.7 ng/µl
3 26.5 ng/µl
1 34.8 ng/µl
OD
A 사 1 29.5 ng/µl 1.79 3.4 ug
평균
Conc.
Blood Sample 34.4 ng/µl (260/280) Total 농도
2
28.8 ng/µl
2.8 ug
2.45
2
B 사
1 33.1 ng/µl
B 사 3 27.9 ng/µl 2.06 3.2 ug
32.4 ng/µl
37.1 ng/µl
BIOFACT 2 1 41.7 ng/µl 1.92 3.9 ug
<Whole Blood> C 사 1 2 2 1 21.8 ng/µl 0.62 2.1 ug
34.8 ng/µl
21.2 ng/µl
A 사 1.79 3.4 ug
2 34.4 ng/µl
BIOFACT Company A Company B Company C 1 33.1 ng/µl
M M B 사 2 32.4 ng/µl 2.06 Total 3.2 ug
OD
Whole Blood Sample Sample Conc. 21.8 ng/µl OD Concentration 평균
Blood
Conc.(260/280)
1
[Average]
0.62
C 사 (260/280) Total 농도
2.1 ug
21.2 ng/µl
1 2 37.1 ng/㎕
BIOFACT 1 37.1 ng/µl 1.92 3.9 ㎍
BIOFACT2 2 41.7 ng/㎕ 1.92 3.9 ug
41.7 ng/µl
1 34.8 ng/㎕
Company A 1 34.8 ng/µl 1.79 3.4 ㎍
A 사 2 34.4 ng/㎕ 1.79 3.4 ug
2 34.4 ng/µl
1 33.1 ng/㎕
Company B 1 33.1 ng/µl 2.06 3.2 ㎍
B 사 2 32.4 ng/㎕ 2.06 3.2 ug
2 32.4 ng/µl
1 21.8 ng/㎕
Company C 1 21.8 ng/µl 0.62 2.1 ㎍
C 사 2 21.2 ng/㎕ 0.62 2.1 ug
2 21.2 ng/µl
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading
Elution volume : 100 ㎕
157
BIOFACT CATALOGUE
Product Information
HiGene™ Genomic DNA Prep Kit For Whole Blood, Bacterium, Plant, Animal Tissue, Fungus [GD141-100]
▶Contents
GD1 WB Bottle
GD2 Spin Column & Collection Tube
PPB (For Plant) Proteinase K
GB RNase A
DNA Hydration Solution Lysozyme
Help B
HiGene™ Genomic DNA Prep Kit For Yeast [GD142-100]
▶Contents
SGD1 Spin Column & Collection Tube
SGD2 Proteinase K
H1 (For Yeast) Lyticase
DNA Hydration Solution Lyticase Suspension Solution
WB Bottle Help B
Reference
1. LI, Mei Jia, et al. Characterization and Pathogenicity of Alternaria vanuatuensis, a New Record from Allium Plants in Korea and China. Mycobiology, 2014, 42.4: 412-415.
2. BACK, Chang-Gi, et al. Development of a Species-speci c PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome
Sequences. The plant pathology journal, 2015, 31.3: 212.
Maker Cat.No. Product Size
GD141-050 HiGene™ Genomic DNA Prep Kit (For Whole Blood, Bacterium, Plant, Animal Tissue, Fungus) 50 prep
GD141-100 HiGene™ Genomic DNA Prep Kit (For Whole Blood, Bacterium, Plant, Animal Tissue, Fungus) 100 prep
BIOFACT
GD142-050 HiGene™ Genomic DNA Prep Kit (For Yeast) 50 prep
GD142-100 HiGene™ Genomic DNA Prep Kit (For Yeast) 100 prep
It is available to purchase individually in case of Spin Column & Collection Tube separately. Please contact us for more details.
158 BIOFACT CATALOGUE
Grinding Bead & Column Type DNA / RNA Preparation
HiGene™ Genomic DNA
Prep Kit For Microorganisms
Extraction of Microorganisms Genomic DNA without Expensive Fragmentation Equipment
HiGene™ Genomic DNA Prep Kit For Microorganisms is a column type kit that can extract high-quality genomic DNA from
gram-positive/negative bacterium, yeast and fungus. The kit includes a BioFACT™ grinding bead tube for e cient dissolution.
Additional enzymes such as high-concentration lyticase or lysozyme are unnecessary except proteinase K and genomic DNA can
be extracted easily and quickly using a general vortex mixer.
Feature
• Spin column type • Column binding capacity : 20 ㎍ DNA / Column
• Membrane : Glass micro ber
Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR and etc.) • Clone library construction
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP, etc.)
Workflow
- HiGene™ Genomic DNA Prep Kit For Microorganisms [GD101-100]
P1
Mechanical Column binding & Elute DNA Pure genomic DNA
lysis washing
Grinding time for each sample using BioFACT™ Grinding Comparison of extraction time
Bead Tube
Grinding Bead Time S. aureus E. coli S. cerevisiae
Sample Material Griding Time (min)
Tube 250
Gram-Negative Bacterium BioFACT™ 4 min. 200
e.g., Escherichia coli, Salmonella typhimurium P1 tube
150
Gram-Positive Bacterium BioFACT™ 12 min.
e.g., Staphylococcus aureus, Listeria monocytogenes P1 tube 100
Yeast BioFACT™ 50
e.g., Saccharomyces cerevisiae 12 min. 0
(If using 50 mg - 250 mg : 15 min. ~ 20 min.) P1 tube Competitor A Competitor B BioFACT
+ Lyticase + Lyticase
Filamentous fungus BioFACT™ 15 min. + Lysozyme + Lysozyme
e.g., Aspergillus spec., Alternaria brassicae P1 tube
The prep time of HiGene™ Genomic DNA Prep Kit for Microorganisms[Grinding Bead &
Column Type] was compared with competitor products by processing Staphylococcus
The prep time of HiGene™ Genomic DNA Prep Kit for Microorganisms[Grinding Bead & Column Type] was compared
aureus, Escherichia coli, and Saccharomyces cerevisiae.
with competitor products by processing Staphylococcus aureus, Escherichia coli, and Saccharomyces cerevisiae.
HiGene™ Genomic DNA Prep Kit shows shorter prep times in all three cases.
HiGene™ Genomic DNA Prep Kit shows shorter prep times in all three cases.
159
BIOFACT CATALOGUE
QC DATA
- Comparison of extraction and PCR amplification efficiency _ Fungus
< Lysis using enzyme method > < Lysis using mechanical method>
M M M M
Genomic DNA
extraction
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
M M
PCR
Amplification
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading
- Comparison of extraction efficiency _ Microalgae filtered through the filter
1 2 3 M
sample ng /㎕ 260 / 280 260 / 230
1 Company A (For soil) 135.8 2.13 0.24
M : 1kb DNA Ladder BIOFACT
1. Company A (For soil) 2 (Lysis using enzyme method) 57.2 1.79 0.81
2. BIOFACT (Lysis using enzyme method) BIOFACT
3 394.5 1.93 2.17
3. BIOFACT (Lysis using mechanical method) (Lysis using mechanical method)
- Comparison of extraction and PCR amplification efficiency from various samples
Bacterium Fungus 1 Fungus 2 Fungus 3 Fungus 4
Gram(-) Gram(+) Yeast
M M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4
Gram(-) : E. coli, Gram(+) : S. aureus, Yeast : S. cerevisiae Fungus 1 : Cercophora sulphurella Fungus 2 : Alternaria [brassicae Fungus 3 : Botryotrichum piluliferum Fungus 4 : Aspergillus niger
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading 1 : Undiluted(5 0 ) 2 : 5 -1 3 : 5 -2 4 : 5 -3 target Gene : 18S rRNA ITS1/ITS4] M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading
Elution volume : 100 ㎕
160 BIOFACT CATALOGUE
DNA / RNA Preparation
- 18S rRNA sequencing analysis _ Fungus
<Cercophora sulphurella> <Alternaria brassicae>
<Botryotrichum piluliferum> <Aspergillus niger>
Product Information
For Microorganisms [GD101-100] HiGene™ Grinding Bead Tube Holder [GBH-012]
▶Contents ▶Contents
BL Help B Grinding Bead Tube Holder
BMB Elution Bu er
BMW1 RNase A
BMW2 Proteinase K
BMW3 P1 (Grinding Bead)
Spin Column & Collection Tube
Maker Cat.No. Product Size
GD101-100 HiGene™ Genomic DNA Prep Kit For Microorganisms 100 prep
BIOFACT
GBH-012 HiGene™ Grinding Bead Tube Holder EA
It is available to purchase individually in case of Spin Column & Collection Tube separately. Please contact us for more details.
161
BIOFACT CATALOGUE
Grinding Bead & Column Type
HiGene™
Genomic DNA Prep Kit For Soil
High Quality Genomic DNA Extraction with Less PCR Inhibitor and Faster Time
HiGene™ Genomic DNA Prep Kit for Soil is a column & grinding bead type kit that can extract high-quality genomic DNA from soil.
The kit includes a BioFACT™ grinding bead tube for e cient dissolution. Additional enzymes such as high-concentration lyticase or
lysozyme are unnecessary except proteinase K and genomic DNA can be extracted easily and quickly using a general vortex mixer.
Extraction process is also more convenient compared to products from other manufacturers. In addition, less PCR inhibitor is extract-
ed compared to the products from other manufacturers, so PCR ampli cation can be done from undiluted genomic DNA.
Feature
• Spin column type • Column binding capacity : 20 ㎍ DNA / Column
• Membrane : Glass microfiber
Application
• PCR (Real-Time PCR & Multiplex PCR) • Genotyping
• NGS (Metagenome sequencing)
Workflow
- HiGene™ Genomic DNA Prep Kit For Soil [GD105-100]
HiGene™ Grinding Bead Holder [GBH-012]
Preparation
Sample grinding
Binding Washing Elution
GeneReady UltraCool [BSH-C2]
162 BIOFACT CATALOGUE
DNA / RNA Preparation
QC DATA
- Comparison of extraction and PCR amplification efficiency between HiGene™ Genomic DNA Prep Kit for Soil
and other company’s prep kit
<Genomic DNA extraction efficiency on various soil>
Company A BIOFACT
M 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 M 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8
Sample vol. : 500mg 1 : Mountain soil 2 : Paddy soil 3 : Field (Acre) soil 4 : Fertilizer soil 5 : Soil, Upstream of the river
6 : Soll, Downstream of the river 7 : Soil, Riverside 8 : Soil, Mud flat
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
< PCR amplification efficiency on various soil>
A : Company A B : BIOFACT (Grinder) C : BIOFACT (Genie 2 vortex) A : Company A B : BIOFACT (Grinder) C : BIOFACT (Genie 2 vortex)
1 2 3 M 1 2 3 M 1 2 3 1 2 3 M 1 2 3 M 1 2 3
Soil,
Mountain soil Upstream
of the river
Soil,
Paddy soil Downstream
of the river
Field (Acre) soil Soil,
Riverside
Fertilizer soil Soil,
Mud flat
A : Company A BIOFACT (Grinder, GeneReady 5m/s, 40sec) BIOFACT (Vortexer, Genie 2 9-10 level at max. strength (600-3200 rpm), 5 mins.)
Genomic DNA Conc. 1 : 5 0 2 : 5 -1 3 : 5 -2 Target Gene (size) : 16s rRNA (1.5kb) M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
Conclusion
In PCR amplification, it can be confirmed that genomic DNA was amplified from its undiluted
concentration as the effect of inhibition is less than that of other kit.
- Comparison of extraction time between HiGene™ Genomic DNA Prep Kit for Soil and other company’s prep kit
BIOFACT 25 mins.
Company A 42 mins.
(mins.) 0 10 20 30 40 50
163
BIOFACT CATALOGUE
Product Information
HiGene™ Genomic DNA Prep Kit For Soil [GD105-100]
▶Contents
SL
SB
SW1
SW2
P1 (Grinding Bead)
Help B
Elution Bu er
Spin Column & Collection Tube
Maker Cat.No. Product Size
BIOFACT GD105-100 HiGene™ Genomic DNA Prep Kit For Soil 100 prep
164 BIOFACT CATALOGUE
Column Type
Solution Type DNA / RNA Preparation
Magnetic Bead Type
Easy Type
HiGene™ Genomic DNA
Magnetic Solution Column Easy
Bead Type Type Type Type
Prep Kit [Solution Type]
High Yield Genomic DNA Preparation from Small Sample Volume
HiGene™ Genomic DNA Prep Kit [Solution Type] is a safe system for isolation of high yield and high quality genomic DNA by salt-
ing-out method, not using existing phenol-chloroform method. This system allows easy scale up and minimizes the DNA fragmenta-
tion which is the problem caused by spin column typed kits or other ltration typed kits.
Feature
• Solution type • High yield
• Easy scale up • Safe and cost-saving kit
Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR and etc.) • Library construction
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP and etc.)
Workflow
- HiGene™ Genomic DNA Prep Kit For Bacterium [GD262-060]
Mixing sample + Protein
+ Cell Lysis Solution Precipitation Ethanol down Washing DNA elution
+ RNase A Solution
QC DATA
- Comparison of gDNA isolation efficiency between HiGene™ Genomic DNA Prep Kit [Solution Type] and
other company's prep kit
<Animal Tissue - Bovine Tissue, 10 mg>
BIOFACT Company A
M M
Bovine Sample Conc. OD(260/280) Total DNA conc.
1 97.9 ng/㎕ 9.7 ㎍
BIOFACT 1.91
2 99.9 ng/㎕ 9.9 ㎍
1 57.0 ng/㎕ 5.7 ㎍
Company A 1.94
2 54.3 ng/㎕ 5.4 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
165
BIOFACT CATALOGUE
<Plant - Lettus, 30 mg>
M BIOFACT Company A M
Lettus Sample Conc. OD(260/280) Total DNA conc.
1 272.9 ng/㎕ 27.3 ㎍
BIOFACT 1.85
2 243.3 ng/㎕ 24.3 ㎍
1 189.5 ng/㎕ 18.9 ㎍
Company A 1.76
2 207.5 ng/㎕ 20.7 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
<Microorganism - Gram- (E.coli)>
M BIOFACT Company A
E.coli Sample Conc. OD(260/280) Total DNA conc.
1 1003.4 ng/㎕ 10.0 ㎍
BIOFACT 2.10
2 1039.2 ng/㎕ 10.3 ㎍
1 989.2 ng/㎕ 9.9 ㎍
Company A 2.06
2 1021.3 ng/㎕ 10.2 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
<Microorganism - Gram+ (Bacillus)>
BIOFACT Company A
M
Bacillus Sample Conc. OD(260/280) Total DNA conc.
1 131.5 ng/㎕ 13.1 ㎍
BIOFACT 2.01
2 137.0 ng/㎕ 13.7 ㎍
1 102.6 ng/㎕ 10.2 ㎍
Company A 1.98
2 95.3 ng/㎕ 9.5 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
<Yeast - Saccharomyces cerevisiae>
M BIOFACT Company A
Saccharomyces Sample Conc. OD(260/280) Total DNA conc.
cerevisiae
1 32.6 ng/㎕ 3.3 ㎍
BIOFACT 2.19
2 33.6 ng/㎕ 3.4 ㎍
1 26.2 ng/㎕ 2.6 ㎍
Company A 2.06
2 28.5 ng/㎕ 2.9 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
166 BIOFACT CATALOGUE
DNA / RNA Preparation
<Human - Tissue Cultured Cell>
M BIOFACT Company A M
Tissue Cultured Sample Conc. OD(260/280) Total DNA conc.
Cell
1 322.1 ng/㎕ 32.2 ㎍
BIOFACT 1.99
2 385.3 ng/㎕ 38.5 ㎍
1 301.4 ng/㎕ 30.1 ㎍
Company A 2.00
2 312.4 ng/㎕ 31.2 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
<Human - Whole Blood>
M BIOFACT Company A M
Whole Blood Sample Conc. OD(260/280) Total DNA conc.
1 60.8 ng/㎕ 6.1 ㎍
BIOFACT 1.84
2 64.4 ng/㎕ 6.4 ㎍
1 27.4 ng/㎕ 2.7 ㎍
Company A 1.76
2 21.6 ng/㎕ 2.1 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
<Human - Saliva>
M BIOFACT Company A M
Saliva Sample Conc. OD(260/280) Total DNA conc.
1 134.8 ng/㎕ 13.5 ㎍
BIOFACT 1.92
2 113.7 ng/㎕ 11.4 ㎍
1 16.9 ng/㎕ 1.7 ㎍
Company A 2.05
2 17.1 ng/㎕ 1.7 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
Know-how for high yield
1. Please use recommended volume / amount of each sample. In case of isolation from large amount of sample, adjust the input
volume of each solution in proportion to the amount of sample.
2. Prepare 80% Ethanol (not provided) in a WB Bottle. Make it fresh before use.
3. Please remove EtOH completely thorough column centrifugation before using DNA Hydration Solution.
4. Add dried enzyme to D.W (Bu er) and mix well. Please store it at -20°C.
5. Please use the product according to its expiration date.
6. Please do not vortexing strongly to avoid DNA degradation during cell lysis step.
7. Storage of Cell lysis solution at lower temperature may cause precipitation of salts due to SDS.
In this case, please use it after thawing in the microwave/dry oven.
167
BIOFACT CATALOGUE
Product Information
HiGene™ Genomic DNA Prep Kit For Blood [GD261-060]
▶Contents
RBC Lysis Solution
Cell Lysis Solution
Protein Precipitation Solution
DNA Hydration Solution
WB Bottle
HiGene™ Genomic DNA Prep Kit For Bacterium [GD262-060] HiGene™ Genomic DNA Prep Kit For Plant [GD263-060]
▶Contents
Cell Lysis Solution ▶Contents
Cell Resuspension Solution Cell Lysis Solution
Protein Precipitation Solution Protein Precipitation Solution
DNA Hydration Solution DNA Hydration Solution
WB Bottle WB Bottle
Lysozyme RNase A
RNase A
HiGene™ Genomic DNA Prep Kit For Animal Tissue, Fungus HiGene™ Genomic DNA Prep Kit For Yeast [GD265-060]
[GD264-060]
▶Contents
▶Contents
Cell Lysis Solution
Cell Lysis Solution
Cell Resuspension Solution
Cell Resuspension Solution
Protein Precipitation Solution
Protein Precipitation Solution
DNA Hydration Solution
DNA Hydration Solution
WB Bottle
WB Bottle
Lyticase
RNase A
Lyticase Suspension Solution
Proteinase K
RNase A
Reference
1. JEON, Sun Jeong; NGUYEN, Thi Thuong Thuong; LEE, Hyang Burm. Phylogenetic Status of an Unrecorded Species of Curvularia, C. spicifera, Based on Current Classi
cation System of Curvularia and Bipolaris Group Using Multi Loci. Mycobiology, 2015, 43.3: 210-217.
2. NAM, Jiyoung, et al. Isolation and NMR Analysis of Antifungal Fengycin A and B from Bacillus amyloliquefaciens subsp. plantarum BC32-1. Bulletin of the Korean
Chemical Society, 2015, 36.5: 1316-1321.
Maker Cat.No. Product Size
GD261-060 HiGene™ Genomic DNA Prep Kit For Whole Blood 60 ㎖ (200 prep)
GD262-060 HiGene™ Genomic DNA Prep Kit For Bacteria 60 ㎖ (200 prep)
BIOFACT GD263-060 HiGene™ Genomic DNA Prep Kit For Plant 60 ㎖ (200 prep)
GD264-060 HiGene™ Genomic DNA Prep Kit For Animal tissue, Fungus 60 ㎖ (200 prep)
GD265-060 HiGene™ Genomic DNA Prep Kit For Yeast 60 ㎖ (200 prep)
168 BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type DNA / RNA Preparation
Magnetic Solution Column Easy
SimpleWay™
Bead Type Type Type Type
gDNA Prep & PCR Set
Direct PCR System for Multiple PCR Reactions from a Single Sample
SimpleWay™ gDNA Prep & PCR Set is composed of prep kit for genomic DNA isolation through simple pretreatment step and master mix
for PCR ampli cation. SimpleWay™ gDNA Prep Set is designed to prepare pure genomic DNA simply and quickly from various samples
using boiling method. Furthermore, included PCR Master Mix is a specially engineered Hot Start enzyme to exhibit high resistance to
many PCR inhibitors for direct PCR and it is highly suitable for ampli cation of less than 1kb DNA fragments. SimpleWay™ gDNA Prep &
PCR Set minimizes the handling error by using a single bu er and multiple PCR reactions can be performed from a single sample through
the kit.
Feature
• Boiling type • Rapid preparation • Safe and economical • Suitable for UDG system
Application
• PCR • Direct PCR • Genotyping
Workflow
- SimpleWay™ gDNA Prep & PCR set I [SW401-100]
Bu er (SLB) Boiling PCR Using Electrophoresis
+ Sample mixing (95℃ 10 min.) Supernatant
QC DATA
- Direct PCR data using SimpleWay™ gDNA Prep & PCR Set I - Comarison of efficiency between SimpleWay™ gDNA Pre
& PCR Set I and other companies' gDNA prep & PCR sets
<Oral Epithelial Cell> <Whole Blood>
D.W 10 ㎕ suspension D.W 30 ㎕ suspension D.W 50 ㎕ suspension Company A Company B BIOFACT
M 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 M 1 2 3 1 2 3 1 2 3
Using collected oral epithelial cell in D.W suspension for genomic DNA preparation Target gene : glo (1 kb)
Target gene : ApoE (389 bp) M : 1kb DNA Ladder
M : 1kb DNA Ladder 1 - 3 : Template volume for PCR amplification
1 - 5 : Template volume for PCR amplification 1 : 1 ㎕ 2 : 3 ㎕ 3 : 5 ㎕
1 : 1 ㎕ 2 : 2 ㎕ 3 : 3 ㎕ 4 : 4 ㎕ 5 : 5 ㎕
169
BIOFACT CATALOGUE
<Hair Root> <Oral Epithelial Cell>
Hair root 1 ea Hair root 2 ea Hair root 4 ea Company A Company B BIOFACT
M 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 M 1 2 3 1 2 3 1 2 3
Target gene : ApoE (389 bp) Target gene : glo (1 kb)
M : 1kb DNA Ladder M : 1kb DNA Ladder
1 - 5 : Template volume for PCR amplification 1 - 3 : Template volume for PCR amplification
1 : 1 ㎕ 2 : 2 ㎕ 3 : 3 ㎕ 4 : 4 ㎕ 5 : 5 ㎕ 1 : 1 ㎕ 2 : 3 ㎕ 3 : 5 ㎕
<Saliva> <Animal Tissue>
D.W 10 ㎕ suspension D.W 30 ㎕ suspension D.W 50 ㎕ suspension Company A Company B BIOFACT
M 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 M 1 2 3 1 2 3 1 2 3
Using collected oral epithelial cell in D.W suspension for genomic DNA preparation Target gene : ApoE (389 bp)
Target gene : glo (600 bp) M : 1kb DNA Ladder
M : 1kb DNA Ladder 1 - 3 : Template volume for PCR amplification
1 - 5 : Template volume for PCR amplification 1 : 1 ㎕ 2 : 3 ㎕ 3 : 5 ㎕
1 : 1 ㎕ 2 : 2 ㎕ 3 : 3 ㎕ 4 : 4 ㎕ 5 : 5 ㎕
Product Information
SimpleWay™ gDNA Prep & PCR SetⅠ
For Whole Blood / Animal Tissue / Saliva / Hair Root / Oral Epithelial Cell [SW401-100]
▶Contents
SLB
Proteinase K
2X PCR Master Mix I
5X Band Helper™
6X Loading Dye
Maker Cat.No. Product Size
BIOFACT SW401-100 SimpleWay™ gDNA Prep & PCR SetⅠ 100 reaction
(For Whole Blood / Animal Tissue / Saliva / Hair Root / Oral Epithelial Cell)
It is available to purchase separately in case of SimpleWay™ gDNA Prep Kit (Easy Type) and SimpleWay™ 2X PCR Master Mix. Please contact us for more details.
170 BIOFACT CATALOGUE
DNA / RNA Preparation
Column Type
Solution Type
Magnetic Bead Type
DaBead™
Easy Type
Magnetic Total RNA Prep Kit
Easy
Solution
Column
Bead Type Type Type Type
High-E ciency Total RNA Extraction using Magnetic Bead
DaBead™ Total RNA Prep Kit allows you to quickly and easily extract total RNA from various plant samples using DaBead™ Magnet-
ic Bead. DaBead™ Magnetic bead is a product developed for nucleic acid puri cation by coating the surface of Super Paramagnetic
Nanoparticle with silica. Because the steps are similar to the column type prep method, it is easy to use even without prior expe-
rience, and it can be used conveniently by minimizing the centrifugation step. It can be used with 1.5 ㎖ Magnetic Stand, 8well
Magnetic pipettor, automatic nucleic acid extraction equipment, and the 96well / 48well Magnetic Press Pipettor.
Feature
• Magnetic Bead type • Extraction from various types of plant samples
• Extraction with a small amount of sample available • Scale up extraction available
• Quick process by minimizing the use of centrifuge (DaBead™ Magnetic Separation Stand, 1.5 ㎖ type)
• Easy to use even without prior experience • Avoiding cross-contamination between samples by using
the single-use consumables included in the kit
Application
• RT, RT-PCR, qRT-PCR • RNase protection assays
• Northern blotting analysis • Translation
Workflow
- DaBead™ Total RNA Prep Kit Prep Kit _ 1.5 ㎖ Magnetic Stand [RP701-100]
Magnet
Cell Lysis & Magnetic Bead Magnetic Bead Magnet
RNA elution
Eluted RNA
precipitation biniding washing & dry DNA elution Eluted DNA
- DaBead™ Total RNA Prep Kit Prep Kit _ 8well Pipettor [RP801S-096]
Cell lysis &
precipitation DNA binding Washing DNA elution
171
BIOFACT CATALOGUE
- DaBead™ Total RNA Prep Kit Prep Kit _ APX-32P [RP801A-096]
Sample Magnetic DNA
Bead
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Bead binding Lysis bu er Washing bu er1,2 Elution bu er Release bead
- DaBead™ Total RNA Prep Kit _ 96well Press Pipettor [RP901-096, RP401-096]
Cell Lysis & DNA binding Washing DNA elution
precipitation
QC DATA
- Comparison of extraction efficiency on various plant samples
Lactuca sativa (Leaf) Brassica napus (Stem) Ixeridium dentatum (Root) Perilla frutescens (Seed)
M BIOFACT Company A M BIOFACT Company A M BIOFACT Company A M BIOFACT Company A
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
- RT-PCR LOD test (PCR inhibition) on various concentrations - qRT-PCR LOD Test (PCR inhibition)
Lactuca sativa Brassica napus Ixeridium dentatum Perilla frutescens
(Leaf) (Stem) (Root) (Seed) Lactuca sativa Brassica napus Ixeridium dentatum Perilla frutescens
(Leaf) (Stem) (Root) (Seed)
M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M 1 2 3 4
M : 1kb DNA Ladder 1 : Undiluted(5 0 ) 2 : 5 -1 3 : 5 -2 4 : 5 -3 Target Gene : 18S rRNA Template : Undiluted(5 0 ) , 5 -1 , 5 -2 , 5 -3 Target Gene : 18S rRNA
** As a result of the Template LOD test, PCR can be performed without PCR Inhibition (High Purity) ** As a result of the Template LOD test, PCR can be performed without PCR Inhibition (High Purity)
172 BIOFACT CATALOGUE
DNA / RNA Preparation
Product Information
DaBead™ Total RNA Prep Kit For Bacterium, Cultured Cell, Animal Tissue, Yeast [RP701-100]
▶Contents
Lysis Bu er Proteinase K
Washing Bu er 1 Lysozyme
Washing Bu er 2 Lyticase
RNase free water DNase I
10X DNase I Bu er Magnetic Bead(RNA)
Lyticase Resuspension Bu er
DaBead™ Total RNA Prep Kit For Plant Tissue [RP703-100]
▶Contents
Lysis Bu er RNase free water
RNA Binding Enhancer 10X DNase I Bu er
Washing Bu er 1 DNase I
Washing Bu er 2 Magnetic Bead(RNA)
Maker Cat.No. Product Size
RP701-100 DaBead™ Total RNA Prep Kit For Bacterium, Cultured Cell, Animal Tissue, Yeast _ 1.5 ㎖ Magnetic stand 100 prep
RP801S-096 DaBead™ Total RNA Prep Kit For Bacterium, Cultured Cell, Animal Tissue, Yeast _ 8well Pipettor 16 prep x 6 plate
RP801A-096 DaBead™ Total RNA Prep Kit For Bacterium, Cultured Cell, Animal Tissue, Yeast _ APX-32P 16 prep x 6 plate
RP901-096 DaBead™ Total RNA Prep Kit For Bacterium, Cultured Cell, Animal Tissue, Yeast _ 96well Press Pipettor 96 prep x 1 Plate
RP401-096 DaBead™ Total RNA Prep Kit For Bacterium, Cultured Cell, Animal Tissue, Yeast _ 48well Press Pipettor 48 prep x 2 plate
BIOFACT
RP703-100 DaBead™ Total RNA Prep Kit For Plant Tissue (Leaf, Stem, Root) _ 1.5 ㎖ Magnetic stand 100 prep
RP803S-096 DaBead™ Total RNA Prep Kit For Plant Tissue (Leaf, Stem, Root) _ 8well Pipettor 16 prep x 6 plate
RP803A-096 DaBead™ Total RNA Prep Kit For Plant Tissue (Leaf, Stem, Root) _ APX-32P 16 prep x 6 plate
RP903-096 DaBead™ Total RNA Prep Kit For Plant Tissue (Leaf, Stem, Root) _ 96well Press Pipettor 96 prep x 1 Plate
RP403-096 DaBead™ Total RNA Prep Kit For Plant Tissue (Leaf, Stem, Root) _ 48well Press Pipettor 48 prep x 2 plate
* Magnetic Bead can be purchased separately.
* Plasticwares : RP701-100, RP703-100 (Not included), Rest of them (Included)
173
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
HiGene™
Type Total RNA Prep Kit
Magnetic Solution Column Easy
Bead Type Type Type
Isolation of Total RNA from Various Samples
HiGene™ Total RNA Prep Kit is intended for isolation of high quality RNA from various samples such as plant, bacteria, animal tissue
and tissue cultured cell using a glass micro ber membrane spin column. It is a safe system which is designed to avoid completely
the use of toxic and hazardous reagents such as phenol/chloroform.
Feature
• Spin column type • Column binding capacity : 20 ㎍ RNA /column
• Membrane : Glass micro ber
Application
• RT, RT-PCR and qRT-PCR • Translation
• Northern blot analysis • Cloning
• RNase protection assays • Poly-A selection
Workflow
- HiGene™ Total RNA Prep Kit Prep Kit [RP101-100]
Mixing RB & Column binding &
Sample Washing Elute RNA Pure Total RNA
QC DATA
- Comparison of total RNA isolation efficiency on various sample scales
<Plant> <Animal Tissue and Cultured Cell> <Bacterium - Gram- >
M 1 2 3 M 1 2 M 1 2 3 M 1 2 3 M 1 2 3
Perilla frutescens (Leaf) Kaki persimmon Bovine liver HEK293F Cells E.coli
M : 1kb DNA Ladder (Leaf, Wax-coated) (Animal tissue) (Animal Cultured cell) M : 1kb DNA Ladder
1 : Company A M : 1kb DNA Ladder M : 1kb DNA Ladder M : 1kb DNA Ladder 1 : Company A
2 : Company B 1 : Company A 1 : Company A 1 : Company A 2 : Company B
3 : BIOFACT 2 : BIOFACT 2 : Company B 2 : Company B 3 : BIOFACT
3 : BIOFACT 3 : BIOFACT
174 BIOFACT CATALOGUE
M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3
Perilla frutescens (Leaf) Kaki persimmon Bovine Liver Bacillus sp. E.coli
M : 1kb DNA Ladder (Leaf, Wax-coated) (Animal tissue) M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : 10 mg M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : 1X10 7 1 : 1X10 7
2 : 50 mg 1 : 10 mg 1 : 10 mg 2 : 1X10 8 2 : 1X10 8
3 : 100 mg 2 : 50 mg 2 : 20 mg 3 : 1X10 9 3 : 1X10 9
3 : 100 mg 3 : 30 mg
* Before DNase I treatment
10 ng
2 ng 0.4 ng
0.08 ng
Gene : GAPDH Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
Product Size : 249 bp ABI 7500 FAST
10 ng
0.4 ng
2 ng
0.08 ng
Gene : B-2M Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
Product Size : 244bp ABI 7500 FAST
1
2
1
2
M
M
E.coli
HEK293F Cells
Perilla Leaf (깻잎)
(Wax-covered leaf)
(Animal tissue)
(Animal Cultured cell)
M : 1kb DNA Ladder
M : 1kb DNA Ladder
M : 1kb DNA Ladder
M : 1kb DNA Ladder
M : 1kb DNA Ladder
1 : A사
1 : A사
1 : A사
1 : A사
1 : A사
2 : B사
2 : B사
2 : BIOFACT
2 : B사
2 : B사
3 : BIOFACT
3 : BIOFACT
3
3 : BIOFACT
3 : BIOFACT
M
3
M
M
M
M 1 1 2 2 3 3 M 1 1 2 2 M 1 1 2 2 3 3 M M M 1 1 1 2 2 2 3 3 3 Persimmon Leaf (감잎) M 1 1 2 2 Bovine Liver (소간) 3 M 1 2 3 M 1 2 3
M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3
Perilla Leaf (깻잎) Persimmon Leaf (감잎) Bovine Liver (소간) HEK293F Cells E.coli
M : 1kb DNA Ladder (Wax-covered leaf) (Animal tissue) (Animal Cultured cell) M : 1kb DNA Ladder
DNA / RNA Preparation
M : 1kb DNA Ladder M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : A사 1 : A사
1 : A사 1 : A사 1 : A사
2 : B사 2 : B사
Perilla frutescens (Leaf) Kaki persimmon Bovine liver HEK293F Cells E.coli
2 : BIOFACT
3 : BIOFACT 2 : B사 2 : B사 3 : BIOFACT
M : 1kb DNA Ladder (Leaf, Wax-coated) (Animal tissue) (Animal Cultured cell) M : 1kb DNA Ladder
3 : BIOFACT
3 : BIOFACT
1 : Company A M : 1kb DNA Ladder M : 1kb DNA Ladder M : 1kb DNA Ladder 1 : Company A
Perilla Leaf (깻잎) Persimmon Leaf (감잎) Bovine Liver (소간) Bacillus sp. E.coli
1 : Company A
2 : Company B 1 : Company A 1 : Company A M : 1kb DNA Ladder (Wax-covered leaf) 2 : Company B (Animal tissue) M : 1kb DNA Ladder M : 1kb DNA Ladder
2 : Company B
3 : BIOFACT 2 : BIOFACT 2 : Company B 1 : 10 mg M : 1kb DNA Ladder 3 : BIOFACT M : 1kb DNA Ladder 1 : 1X10 7 1 : 1X10 7
3 : BIOFACT 3 : BIOFACT 1 : 10 mg 1 : 10 mg
2 : 50 mg
- Comparison of total RNA isolation efficiency on various sample scales 2 : 50 mg 2 : 20 mg 2 : 1X10 8 2 : 1X10 8
3 : 1X10 9
3 : 100 mg
3 : 1X10 9
2
M 1 2 3 M 1 2 3 M 1 2 3 M 1 3 : 100 mg 3 M 1 2 3 3 : 30 mg
<Plant> <Animal Tissue> <Bacterium - Gram+ >
M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 * 상기 결과는 모두 DNase I 처리 전 입니다.
Perilla Leaf (깻잎) Persimmon Leaf (감잎) Bovine Liver (소간) Bacillus sp. E.coli
M : 1kb DNA Ladder (Wax-covered leaf) (Animal tissue) M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : 10 mg M : 1kb DNA Ladder M : 1kb DNA Ladder 1 : 1X10 7 1 : 1X10 7
2 : 50 mg 1 : 10 mg 1 : 10 mg 2 : 1X10 8 2 : 1X10 8
Perilla frutescens (Leaf) Kaki persimmon Bovine Liver Bacillus sp. E.coli
2 : 20 mg
2 : 50 mg
3 : 100 mg 3 : 1X10 9 3 : 1X10 9
M : 1kb DNA Ladder (Leaf, Wax-coated) (Animal tissue) M : 1kb DNA Ladder M : 1kb DNA Ladder
3 : 30 mg
3 : 100 mg
1 : 10 mg M : 1kb DNA Ladder M : 1kb DNA Ladder 1 : 1X10 7 1 : 1X10 7
2 : 50 mg 1 : 10 mg 1 : 10 mg 2 : 1X10 8 2 : 1X10 8
3 : 100 mg 2 : 50 mg 2 : 20 mg 3 : 1X10 9 10 ng 3 : 1X10 9
3 : 100 mg 3 : 30 mg * 상기 결과는 모두 DNase I 처리 전 입니다.
0.4 ng
2 ng
* Before DNase I treatment
0.08 ng
* Before DNase I treatment
Gene : GAPDH Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
- Amplification efficiency of RNA using HiGene™ Total RNA Prep Kit (qRT-PCR)
Product Size : 249 bp ABI 7500 FAST
10 ng 10 ng
0.4 ng
2 ng 0.4 ng 2 ng
[nt] [nt] 0.08 ng
10 ng 0.08 ng Plant tissue (Leek) Animal tissue (Mouse Liver)
Ladder
2 ng 0.4 ng
Gene : GAPDH Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng Gene : B-2M Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
0.08 ng
Product Size : 249 bp ABI 7500 FAST Product Size : 244bp ABI 7500 FAST
Gene : GAPDH Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
Product Size : 249 bp ABI 7500 FAST
10 ng
0.4 ng
2 ng
- Comparison of migration pattern between HiGene™ Total RNA Prep Kit (Ver 2.0) and other companies' total RNA Prep kits.
0.08 ng
10 ng Plant tissue(Leek) : 40 mg Animal tissue(Mouse Liver) : 20 mg
0.4 ng
Electrophoretic trace 2 ng
0.08 ng
[nt] Gene : B-2M Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
[nt]
Plant tissue (Leek)
Animal tissue (Mouse Liver)
Product Size : 244bp ABI 7500 FAST
Plant tissue (Leek)
Ladder Company A BIOFACT Company B Company C Company A BIOFACT Company B Company C
Gene : B-2M Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
Product Size : 244bp ABI 7500 FAST
Company A BIOFACT Company B Company C
BIOFACT
A 사
C 사
B 사
Animal tissue (Mouse Liver)
Compan BIOFACT B 사 C 사
A 사y A BIOFACT Company B Company C
Plant tissue(Leek) : 40 mg Animal tissue(Mouse Liver) : 20 mg
Plant tissue (Leek)
175
BIOFACT CATALOGUE
A 사 BIOFACT B 사 C 사
Animal tissue (Mouse Liver)
A 사 BIOFACT B 사 C 사
Product Information
HiGene™ Total RNA Prep Kit [RP101-050]
▶Contents
RB
RW
Proteinase K
Lysozyme
RNase Free Water
Capsule Column & Collection Tube
HiGene™ HiFilter & Collection Tube [RP119-100]
▶Contents
HiFilter & Collection Tube
▶Feature
Prevention of column-blockage through cell debris-removal
Increase of nal RNA isolation yield
Non binding capacity of DNA/RNA
Maker Cat.No. Product Size
HiGene™ Total RNA Prep kit
RP101-050 50 prep
(For Plant Tissue, Animal Tissue, Bacterium, Tissue Culture Cell)
HiGene™ Total RNA Prep kit
RP101-100 (For Plant Tissue, Animal Tissue, Bacterium, Tissue Culture Cell) 100 prep
BIOFACT
RP117-20h HiGene™ DNase I (For 500 reactions) 2,000 U
RP118-100 HiGene™ HiSpin Capsule Column with Collection tube 10 ea x 10 pack
RP119-100 HiGene™ HiFilter column with collection tube 50 ea × 2 bottle
176 BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type DNA / RNA Preparation
Easy Type
Magnetic DaBead™ Easy
Solution
Column
Bead Type Type Type Type
Plasmid Prep Kit
High-E ciency Plasmid Extraction using Magnetic Bead
DaBead™ Plasmid Prep Kit is a kit for extracting Plasmid DNA using Magnetic Bead. DaBead™ Magnetic bead is designed for
nucleic acid puri cation by coating the surface of Super Paramagnetic Nanoparticle with silica. As the extraction process
are similar to the column typed method, it is easy to use even without prior experience, and it can be used conveniently by
minimizing the centrifugation step. DaBead™ Plasmid Prep Kit is designed for plasmid DNA extraction in any scale (mini,
midi and maxi prep) using one stand (DaBead™ Magnetic Separation Stand).
Feature
• Magnetic Bead type • Easy to use even without prior experience with simple puri cation steps
• Various scaled extraction (Mini, midi & maxi prep) • Cell debris removal filter included (Optional)
Specifications
Mini scale Midi scale Maxi scale
High Copy 1 ~ 3 ㎖ 20 ~ 50 ㎖ 50 ~ 100 ㎖
Culture Volume
Low Copy 3 ~ 5 ㎖ 50 ~ 100 ㎖ 100 ~ 200 ㎖
Typical Yield < 20㎍ > 200㎍ > 500㎍
Workflow
- DaBead™ Plasmid Prep Kit _ 1.5 ㎖ Magnetic Stand [PM751-100]
DNA Binding Transfer
< PM751> Magnetic Bead eluted DNA
Transfer of
supernatant after
Cfg.
Lysis & protein precipitation
Cell harvest DNA binding & washing DNA elution
& remove cell debris
< PM753 - Cell Debris Removal Syringe Filter Type >
DNA Binding Transfer
Magnetic bead eluted DNA
Cell debris ml 20 ml 20
removal 15 15
syringe filter 10 5 10 5
Lysis & protein precipitation
Cell harvest DNA binding & washing DNA elution
& remove cell debris
177
BIOFACT CATALOGUE
ml 20 15 10 5 ml 20 15 10 5
- DaBead™ Plasmid Prep Kit _ 8well Pipettor [PM851S-096]
Cell lysis & DNA binding Washing DNA elution
precipitation
- DaBead™ Plasmid Prep Kit _ APX-32P [PM851 A-096]
Sample Magnetic DNA
Bead
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Bead binding Lysis bu er Washing bu er1,2 Elution bu er Release bead
- DaBead™ Plasmid Prep Kit _ 96well Press Pipettor [PM951-096, PM451-096]
Cell Lysis &
precipitation DNA binding Washing DNA elution
QC DATA
- Comparison of extraction efficiency on various scales
<Mini Prep> <Midi Prep>
Column Magnetic Bead Column Magnetic Bead BIOFACT Magnetic Bead
M 1 2 3 4 5 M M 1 2 3 M M Mini Midi Maxi
M
M : 1kb DNA Ladder M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : BIOFACT Column Type 2 : BIOFACT 3 : Company A 1 : Company A 2 : Company B 3 : BIOFACT
4 : Company B 5 : Company C
- Magnetic Bead Type & Cell Debris Removal Syringe Filter
<Midi Prep>
Low Copy High Copy
1 2 1 2 1 2
M M M M M
※ 1% agarose gel, 2㎕ loading
※ Total elution volume; Mini(100 ㎕), Midi(2 ㎖), Maxi(2 ㎖)
M : 1kb DNA Ladder 1 : Midi 2 : Maxi 1 : Company A 2 : BIOFACT
178 BIOFACT CATALOGUE
DNA / RNA Preparation
- Sequencing analysis
High quality sequencing data has been obtained through extraction using DaBead™ Plasmid Prep Kit.
- Digestion efficiency of restriction enzyme - How to remove cell debris
M 1 2 3 4 M M 1 2 3 4 M [PM751] : Debris removed using a centrifuge
[PM753] : Debris removed using syringe filter
M : 1Kb DNA Ladder 1 : undigested plasmid DNA(4,729bp) M : 1Kb DNA Ladder 1 : undigested plasmid DNA (5,166bp)
2 : BamHI 3 : StuI 4 : BamHI, StuI 2 : BamHI 3 : EcoRI 4 : BamHI, EcoRI
Product Information
DaBead™ Plasmid Prep Kit [PM751-100] DaBead™ Plasmid Prep Kit
(Cell Debris Removal Syringe Filter) [PM753-010]
▶Contents ▶Contents
MP1 MW2 (empty bottle) MP1 MW2 (empty bottle)
MP2 MW1 Additive MP2 MW1 Additive
MP3 ME MP3 ME
MW1 RNase A MW1 RNase A
Magnetic Bead Magnetic Bead Debris Removal Syringe Filter
Maker Cat.No. Product Size
PM751-100 DaBead™ Plasmid Prep Kit - Mini, Midi, Maxi 100 prep
PM851S-100 DaBead™ Plasmid Prep Kit _ 8well Pipettor 16 prep x 6 plate
PM851A-100 DaBead™ Plasmid Prep Kit _ APX-32P Auto prep machine 16 prep x 6 plate
BIOFACT PM951-096 DaBead™ Plasmid Prep Kit _ 96well Press Pipettor 96 prep x 1 Plate
PM451-096 DaBead™ Plasmid Prep Kit _ 48well Press Pipettor 48 prep x 2 plate
PM753-010 DaBead™ Plasmid Prep Kit (Magnetic Bead Type & Cell Debris Removal Syringe Filter ) - Midi, Maxi 10 prep
PM770-010 HiCell Debris Removal Syringe Filter - Midi, Maxi 10 ea
* Prep Size: Mini(100 tests), Midi(10 tests), Maxi(5 tests) * Magnetic Bead can be purchased separately.
* Plasticwares : PM751-100, PM753-010, PM770-010 (Not included), Rest of them (Included)
179
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
HiGene™
Magnetic Solution Column Easy
Bead Type Type Plasmid Mini Prep Kit (Ver.2.0)
Type
Type
Convenient and Reliable Isolation of High Quality Plasmid DNA in 2 Steps
HiGene™ Plasmid Mini Prep Kit (Ver. 2.0) is a upgraded kit for time-saving and convenient use. Isolation procedure has been reduced and
simpli ed in two steps through application of alkaline lysis method and reduction of clearing time of lysate. HiGene™ Plasmid Mini Prep Kit
(Ver. 2.0) is designed to isolate over 20㎍ high purity plasmids as a spin column type and it is suitable for isolation from low copy plasmid to
large sized circular DNA such as fosmid and BAC clone. Isolated plasmid DNA is applicable for various molecular biological applications such
as PCR, cloning, general sequencing and in vitro transcription. Included, Blue indicator, is a pH indicator which helps users visually check the
pH changes during separation process for plasmids.
Feature
• Spin column type • Sequencing grade
• Membrane : Glass microfiber • Low copy plasmid prep
• Column binding capacity : 20 ㎍ DNA / column • Large size circular DNA (Fosmid, BAC clone) prep
Workflow
- HiGene™ Plasmid Mini Prep Kit [PM105-100]
Cell resuspension & Neutralization Column binding & Elute plasmid Pure plasmid DNA
lysis (Blue Indicator : Blue) (Blue Indicator : Colorless) washing column DNA
QC DATA
- Comparison of isolation efficiency on various plasmid sizes between HiGene™ Plasmid Mini Prep Kit (Ver.2.0)
and other companies' prep kits
Company A BIOFACT
A사
BIOFACT사
M Company A Company B Company C BIOFACT M Company A Company B Company C BIOFACT M 1 2 3 1 2 3 M
B사
BIOFACT사
C사
A사
C사
BIOFACT사
A사
B사
pET21b pMW118 Fosmid
M : 1Kb DNA ladder 1% agarose Gel 5 ㎕ loading 1 : Fosmid + insert 14kb Prep
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
1 : Fosmid + insert 14kb Prep
G : G사 Q : Q사 I : I사 B : BIOFACT 2 : Fosmid
2 : Fosmid
3 : Fosmid + insert 14kb DNA digestion
3 : Fosmid + insert 14kb DNA digestion
180 BIOFACT CATALOGUE
M 1 2 3 4 M M 1 2 3 4 M M 1 2 3 4 M
pUC19 (High Copy) pET21b (Low Copy) pSTV28 (Low Copy)
M : 1kb Plus DNA Ladder 1% agarose Gel 5 ㎕ loading
1 : A사 (3 Step System) 2 : B사 (3 Step System) 3 : C사 (3 Step System) 4 : BIOFACT (2 Step System)
BIOFACT사
A사
M
M
C사
A사
BIOFACT사
B사
C사
B사
A사
BIOFACT사
1
M
2
1
M
3
3
2
pMW118
pET21b
Fosmid
M : 1Kb DNA ladder 1% agarose Gel 5 ㎕ loading
1 : Fosmid + insert 14kb Prep
2 : Fosmid
G : G사 Q : Q사 I : I사 B : BIOFACT
3 : Fosmid + insert 14kb DNA digestion
DNA / RNA Preparation
- Comparison of isolation yield on various plasmid copy numbers between HiGene™ Plasmid Mini Prep Kit (Ver.2.0),
2 Step system and other companies' prep kits (column type), 3 step system
M 1 2 3 4 M M 1 2 3 4 M M 1 2 3 4 M
pUC19 (High Copy) pET21b (Low Copy) pSTV28 (Low Copy)
M : 1kb Plus DNA Ladder 1% agarose Gel 5 ㎕ loading
M : 1kb DNA Ladder 1% agarose Gel 5 ㎕ loading
1 : A사 (3 Step System) 2 : B사 (3 Step System) 3 : C사 (3 Step System) 4 : BIOFACT (2 Step System)
1 : Company A (3 Step System) 2 : Company B (3 Step System) 3 : Company C (3 Step System) 4 : BIOFACT (2 Step System)
- Know for high yield
1. Cell culture time → At least 12 - 16 hrs
2. Culture volume → 3 ㎖ ~ 5 ㎖
3. Prepare 80% EtOH in a WB bottle and make fresh each time.
4. B2 Bu er mixed with RNase A (20 ㎎ / ㎖) should be stored at 4℃.
5. Please completely remove EtOH before using elution bu er.
6. Please use the kit prior to the expiration date.
7. Avoid vigorous vortexing during lysis step to prevent degradation of DNA.
8. Isolation e ciency can be increased when using EB bu er after pre-heating treatment for 10mins at 50℃ during DNA elution step.
(Especially, in case of long fragment)
9. In case it is di cult to separate the debris from supernatant, please perform again after incubation at 4℃ or incubation on ice.
10. When washing using NWB without ethanol, extraction time can be shortened by skipping column-idle step.
- Blue Indicator (Optional)
After B2 buffer
treatment
B1 buffer
Blue Indicator is a pH indicator which helps users visually check the pH changes during separa- B1 Bu er B2 Bu er 처리 후
tion process for plasmids. Users easily can use it after mixing B1 bu er before experiment. Blue 구성품목 (Contents)
Indicator in B1 bu er becomes transparent after B2 bu er step and it doesn't a ect the extraction B1 Spin Column & Collection Tube
e ciency of plasmid isolation. B2 RNase A
EB Blue Indicator
WB Bottle
Product Information
HiGene™ Plasmid Mini Prep Kit (Ver.2.0) [PM105-100]
▶Contents
B1 Spin Column & Collection Tube
B2 RNase A
EB Blue Indicator
NWB Help B
WB Bottle
Reference
1. CHOI, Yeon Hee, et al. A LuxR-type Transcriptional Regulator, PsyR, Coordinates Regulation of Pathogenesis-related Genes in Pseudomonas syringae pv. tabaci.
Journal of Life Science, 2015, 25.2: 136-150.
2. KIM, Dooil, et al. Escherichia coli ASKA Clone Library Harboring tRNA-Speci c Adenosine Deaminase (tadA) Reveals Resistance towards Xanthorrhizol.
Molecules, 2015, 20.9: 16290-16305.
Maker Cat.No. Product Size
PM105-100 HiGene™ Plasmid Mini Prep Kit (Ver. 2.0) 100 prep
BIOFACT
PM105-200 HiGene™ Plasmid Mini Prep Kit (Ver. 2.0) 200 prep
It is available to purchase individually in case of Spin Column & Collection Tube separately.
181
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
Magnetic DaBead™ Easy
Column
Solution
Bead Type Type Type Type
PCR Purification Kit
Small and Large Quantities of Sample Puri cation in One Kit
DaBead™ PCR Puri cation Kit is a kit to purify PCR products using Magnetic Bead. As the extraction process are similar to the column
typed method, it is easy to use even without prior experience, and it can be used conveniently by minimizing the centrifugation
step.
Feature
• Magnetic Bead type • Puri cation step without a centrifuge
• PCR product purification of various sizes • The number of samples adjustable
• Easy to use through simple purification steps
Application
• General sequencing • Restriction enzyme digestion
• Cloning
Workflow
- DaBead™ PCR Purification Kit [PP755-200]
Magnet
Mixing bu er & Magnetic Bead Magnetic Bead Magnet Eluted RNA
Cell Lysis &
PCR Product DNA elution Eluted cell Free DNA
precipitation biniding washing & dry
- DaBead™ PCR Purification Kit _ 8well Pipettor [PP855S-096]
Mixing bu er & DNA binding Washing DNA elution
PCR Product
182 BIOFACT CATALOGUE
DNA / RNA Preparation
- DaBead™ PCR Purification Kit _ 8well Pipettor [PP855A-096]
Sample Magnetic DNA
Bead
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Bead binding Lysis bu er Washing bu er1,2 Elution bu er Release bead
- DaBead™ PCR Purification Kit _ 96well Press Pipettor [PP955-096, PP455-096]
Mixing bu er & DNA binding Washing DNA elution
PCR Product
- DaBead™ PCR Purification Kit _ 96well Magnetic Stand [PP755-200]
QC DATA
- Comparison of PCR purification efficiency between column method and Magnetic Bead method
<0.1 ~ 3 kb> <0.1 ~ 10 kb>
Column Magnetic Bead Column Magnetic Bead
M 1 2 3 4 5 M 1 2 3 4 5
M
M : 1kb DNA Ladder 1 : BIOFACT Column Type 2 : BIOFACT 3 : Company A 1 : BIOFACT Column Type 2 : BIOFACT 3 : Company A
4 : Company B 5 : Company C 4 : Company B 5 : Company C
183
BIOFACT CATALOGUE
< Comparison of Sequencing analysis after PCR purification between column method and Magnetic Bead method >
<Column Type> <Bead Type>
* High quality sequencing data has been obtained through purification using DaBead™ PCR Purification Kit.
- Comparison of purification efficiency on various extraction types
< PCR purification of large number of samples using 96well press pipettor >
A B C D A B
M
M
M
M: 1kb DNA Ladder
A : Before purification B : Column C : FB Plate D : 96well press pipettor
* DNA recovery rate is similar with existing methods
M: 1kb DNA Ladder A : Before PCR purification B : After PCR purification 후
* High quality sequencing data has been obtained through purification using
DaBead™ PCR Purification Kit & DaBead™ 96well Magnetic Press Pipettor
- Comparison of cloning efficiency between column method Colony PCR results
and Magnetic Bead method
<Column Type>
PCR product is subjected to PCR puri cation under the same conditions using Col- NTC
umn Type and Magnetic Bead Type, and then check whether target insert DNA is
inserted through cloning and colony PCR after PCR puri cation through column
method and magnetic bead method.
<Column Type> <Magnetic Bead Type>
<Magnetic Bead Type>
NTC
* As a result of cloning into a product purified by the above two types, it was
confirmed that the insertion rate of insert DNA was similar between two types
184 BIOFACT CATALOGUE
DNA / RNA Preparation
- Removal of primer dimer during sequencing & cloning step
1. Accurate results can be obtained when sequencing step.
→ Through removal of primer dimer, it can avoid double peaks due to the dimer peak at the front part during sequencing step.
<Before Dimer Removal>
Dimer
<After Dimer Removal>
Dimer
2. Cloning e ciency of the target gene can be increased.
→ The probability of cloning of the dimer is higher than that of the target gene as cloning e ciency is higher for smaller sizes.
Cloning should be performed after removal of primer dimer.
Product Information
DaBead™ PCR Purification Kit [PP755-200]
▶Contents
MU MW1 Additive
MPW ME
MW1 Magnetic Bead
MW2 (empty bottle)
Reference
Kyeong-Ryeol Lee, et al. Increasing Monounsaturated Fatty Acid Contents in Hexaploid Camelina sativa Seed Oil by FAD2 Gene Knockout Using CRISPR-Cas9. Front.
Plant Sci., 29 June 2021.
Maker Cat.No. Product Size
PP755-200 DaBead™ PCR Puri cation Kit _ 1.5 ㎖ Magnetic stand 200 prep
PP855S-096 DaBead™ PCR Puri cation Kit _ 8well Pipettor 16 prep x 6 plate
BIOFACT PP855A-096 DaBead™ PCR Puri cation Kit _ APX-32P Auto prep machine 16 prep x 6 plate
PP955-096 DaBead™ PCR Puri cation Kit _ 96well Press Pipettor 96 prep x 1 Plate
PP455-096 DaBead™ PCR Puri cation Kit _ 48well Press Pipettor 48 prep x 2 plate
* Magnetic Bead can be purchased separately.
* Plasticwares : PP755-200 (Not included), Rest of them (Included)
185
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
Magnetic DaBead™ Easy
Solution
Column
Bead Type Type Type Type
Size-Selective Cleanup Kit
Puri cation of DNA fragments of the desired size
DaBead™ Size-Selective Cleanup Kit is a kit for purifying DNA fragments of a desired size, such as library construction for NGS
using magnetic beads.
Feature
• Magnetic Bead type • Puri cation step without a centrifuge
• PCR product purification of various sizes • The number of samples adjustable
• Easy to use through simple purification steps
Application
• NGS cleanup, PCR cleanup, including size selection
Workflow
-1.5 ㎖ Magnetic Separation Stand Magnet Magnet
Magnet
Mixing bu er & Magnetic Bead Magnetic Bead DNA elution Magnet Eluted cell Free DNA
Cell Lysis &
Eluted RNA
PCR Product biniding washing & dry
precipitation
- 96well Magnetic Separation Stand
Lysis & protein precipitation
Cell harvest DNA binding & washing DNA elution
& remove Cell debris
186 BIOFACT CATALOGUE
DNA / RNA Preparation
- Guideline [Small Size Removal Condition] - Guideline [Dual Size Selective Condition]
PCR sample vol.: 50 ㎕ based PCR sample vol.: 50 ㎕ based
Approximate Size Cuto Ratio(v/v) Volume(㎕) Approximate First Selection Second Selection
Target size
1000 bp 0.6V 30.0 Ratio(v/v) Volume(㎕) Ratio(v/v) Volume(㎕)
800 bp 0.65V 32.5 700 bp 0.6V 30.0 0.8V 10
700 bp 0.7V 35.0 600 bp 0.65V 32.5 0.85V 10
600 bp 0.75V 37.5
500 bp 0.8V 40.0 550 bp 0.7V 35.0 0.9V 10
400 bp 0.9V 45.0 450 bp 0.75V 37.5 1.0V 12.5
300 bp 1.0V 50.0 350 bp 0.8V 40.0 1.3V 25
200 bp 1.3V 65.0
150 bp 1.5V 75.0 250 bp 0.9V 45.0 1.5V 30
QC DATA
- Comparison of purification - Comparison of extraction - Comparison of extraction
efficiency on various small fragments efficiency on various PCR product sizes efficiency on various DNA sizes
BIOFACT Company A BIOFACT Company A
0.65/0.85V 0.7/0.9V 0.75/1.0V 0.8/1.3V 0.9/1.5V 1.24/1.6V 1.1/1.45V 1.05/1.4V 1.0/1.3V 0.95/1.25V 0.9/1.5V 0.8/1.3V 0.75/1.0V 0.7/0.9V 0.65/0.85V 1.24/1.6V 1.1/1.45V 1.05/1.4V 1.0/1.3V 0.95/1.25V
M 0.5V 0.6V 0.65V 0.7V 0.75V 0.8V 0.9V 1.0V 1.3V 1.5V M M Input M M Input M
M : 1kb DNA Ladder 3% Agarose gel 10㎕ loading M : 1kb DNA Ladder 3% Agarose gel 10㎕ loading M : 1kb DNA Ladder 3% Agarose gel 10㎕ loading
Product Information
DaBead™ Size-Selective Cleanup Kit [NC701-100]
▶Contents
Size-Selective Reagent (Magnetic Bead)
Washing Bu er
Elution Bu er
Maker Cat.No. Product Size
BIOFACT NC701-100 DaBead™ Size-Selective Cleanup Kit 100 prep
187
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
Magnetic Solution Easy
DaBead™Column
Bead Type Type Type Type
Gel Extraction Kit
DNA Fragment Puri cation Using Magnetic Bead
DaBead™ Gel Extraction Kit is a kit that can perform gel extraction using Magnetic Bead. As the extraction process are similar to the
column typed method, it is easy to use even without prior experience, and it can be used conveniently by minimizing the centrifu-
gation step.
Feature
• Magnetic Bead type • Puri cation step without a centrifuge
• DNA fragment purification of various sizes • Easy to use through simple purification steps
Application
• General sequencing • Cloning • Restriction enzyme digestion
Workflow
- DaBead™ Gel Extraction Kit [GP756-200]
Magnet
Mixing bu er & Magnetic Bead Magnetic Bead DNA elution Magnet Eluted DNA
DNA fragment biniding washing & dry
QC DATA
- Comparison of purification efficiency between gel extraction using Magnetic Bead method and column method
Column Magnetic Bead
M 1 2 3 M
M : 1kb DNA Ladder
<1.2 kb> 1: BIOFACT Column type 2: Company A 3: BIOFACT
188 BIOFACT CATALOGUE
DNA / RNA Preparation
- Comparison of Sequencing analysis after gel extraction between column method and Magnetic Bead method
<Column Type>
<Bead Type>
High quality sequencing data has been obtained through gel extraction
using DaBead™ Gel Extraction Kit.
Product Information
DaBead™ Gel Extraction Kit [GP756-200]
▶Contents
MG
MGW
MW1
MW2 (empty bottle)
ME
Magnetic Bead
Maker Cat.No. Product Size
BIOFACT GP756-200 DaBead™ Gel Extraction Kit 200 prep
* Magnetic Bead can be purchased separately.
189
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
DaBead™ Sequencing Reaction
Magnetic Solution Column Easy
Bead Type Type Type Type
Clean Up Kit
Puri cation of BigDye® Terminator using Magnetic Beads
DaBead™ Sequencing Reaction Clean Up Kit is designed to quick and easy removal of BigDye® Terminator and salt using
Magnetic Bead.
Feature
• Magnetic Bead type • Purification step without a centrifuge
• Purification of a small amount of sample due • Removal of dye blob
to its high binding capacity
Application
• Sequencing
QC DATA
- Comparison of dye blob removal between Magnetic Bead method and existing EDTA/NaOAc method
< EDTA/NaOAc Method>
Height : 2000
< Magnetic Bead Method >
Height : 6000
190 BIOFACT CATALOGUE
DNA / RNA Preparation
- Comparison of signal height between Magnetic Bead method and existing EDTA/NaOAc method
< EDTA/NaOAc Method>
Height : 2500
< Magnetic Bead Method >
Height : 8000
Product Information
DaBead™ Sequencing Reaction Clean Up Kit [SC711-500]
▶Contents
Sequencing Reaction Clean Up Solution
Magnetic Bead
Maker Cat.No. Product Size
SC711-500 DaBead™ Sequencing Reaction Clean Up kit 500 prep
SC711-25h DaBead™ Sequencing Reaction Clean Up kit 2,500 prep
BIOFACT
SC711-10k DaBead™ Sequencing Reaction Clean Up kit 10,000 prep
SC711-50k DaBead™ Sequencing Reaction Clean Up kit 50,000 prep
* DaBead™ Sequencing Reaction Clean Up Kit is composed of Magnetic Bead and Sequencing Reaction Clean Up Solution.
191
BIOFACT CATALOGUE
Column Type
Solution Type
Magnetic Bead Type
Easy Type
HiGene™
Gel & PCR Purification System
Magnetic Solution Column Easy
Bead Type Type Type Type
Gel Extraction and PCR Puri cation in One Kit
HiGene™ Gel & PCR Puri cation System is a spin column typed kit which has dual functions of gel extraction and PCR Puri cation. It
provides the high puri cation system of high yield DNA from 100 bp to 14 kb, and it has an advantage of selective puri cation system
of the two conditions (high yield and primer-dimer removal) according to the application needs. HiGene™ Gel & PCR Puri cation
System is suitable to obtain high quality DNA fragments through high salt bu er and glass micro ber membrane.
Feature
• Spin column type • Puri cation size : 100 bp ~ 14 kb
• Membrane : Glass micro ber • Recovery yield : above 80% (50 ~ 70 %, above 5kb)
• Column binding capacity : 20 ㎍ DNA/column • Sequencing grade
Application
• General sequencing • Restriction enzyme digestion
• Cloning
Workflow
- HiGene™ Gel & PCR Purification System [GP104-100]
or
Mixing Mixing & melting
UB & PCR product UB & Gel Block Column binding Washing column Elute DNA Pure DNA
→ melting
QC DATA
- Comparison of PCR purification efficiency between HiGene™ Gel & PCR Purification System and other companies'
PCR purification kits
<100bp> <1kb>
1 2 3 4 1 2 3 4
M M M M
M : 1kb DNA Ladder 1 : Company A 2 : Company B M : 1kb DNA Ladder 1 : Company A 2 : Company B
3 : Company C 4 : BIOFACT 3 : Company C 4 : BIOFACT
192 BIOFACT CATALOGUE
DNA / RNA Preparation
<5kb> <10kb>
M 1 2 3 4 M M 1 2 3 4 M
M : 1kb DNA Ladder 1 : Company A 2 : Company B M : 1kb DNA Ladder 1 : Company A 2 : Company B
3 : Company C 4 : BIOFACT 3 : Company C 4 : BIOFACT
- Comparison of gel extraction efficiency between HiGene™ Gel & PCR Purification System and other companies' gel extraction kits
<100bp> <1kb>
M 1 2 3 4 M M 1 2 3 4 M
M : 1kb DNA Ladder 1 : Company A 2 : Company B M : 1kb DNA Ladder 1 : Company A 2 : Company B
3 : Company C 4 : BIOFACT 3 : Company C 4 : BIOFACT
<5kb> <10kb>
M 1 2 3 4 M M 1 2 3 4 M
M : 1kb DNA Ladder 1 : Company A 2 : Company B M : 1kb DNA Ladder 1 : Company A 2 : Company B
3 : Company C 4 : BIOFACT 3 : Company C 4 : BIOFACT
- Comparison of PCR purification between HiGene™ Gel & PCR Purification System and other companies' PCR purification kits
on dimer removal condition
M C 1 2 3 M M C 1 2 3 M
M : 1kb DNA Ladder
C : Original Sample(before purification)
1 : BIOFACT
2 : Company A
3 : Company B
Primer dimer
193
BIOFACT CATALOGUE
- Comparison of PCR purification efficiency using HiGene™ Gel & PCR Purification System between dimer removal and
high yield condition
M C 1 2 M
TNFR1 Gene
M : 1kb DNA Ladder
C : Control (before PCR Purification)
1 : HiGene™ Gel & PCR Purification System (Dimer removal condition)
2 : HiGene™ Gel & PCR Purification System (High yield condition)
• Dimer Removal Condition
1. Removal of primer dimer in PCR product regardless of size
2. Puri cation for 100 bp – 5 kb sized PCR product
• High Yield Condition
1. Puri cation for less than 100 bp sized PCR product
2. Puri cation for more than 5 kb DNA fragment
3. Gel extraction to obtain target band only
- Removal of primer dimer during sequencing & cloning step
1. Accurate results can be obtained when sequencing step.
→ Through removal of primer dimer, it can avoid double peaks due to the dimer peak at the front part during sequencing step.
<Before Dimer Removal>
Dimer
<After Dimer Removal>
Dimer
2. Cloning e ciency of the target gene can be increased.
→ The probability of cloning of the dimer is higher than that of the target gene as cloning e ciency is higher for smaller sizes.
Cloning should be performed after removal of primer dimer.
194 BIOFACT CATALOGUE
DNA / RNA Preparation
- Know-how for high yield
1. Prepare 80% EtOH in a WB bottle and make fresh each time.
2. Place the gel block into UB Bu er and mix them thoroughly by pipetting.
3. EtOH should be completely removed before elution step.
4. Choose the appropriate method (Dimer removal / High yield) according to each puri cation purpose.
(Please perform high yield method in case of gel extraction.)
* In order to increase the e ciency during gel extraction method,
- It is recommended to use high quality agarose.
- Place the gel block into UB Bu er and dissolve them thoroughly at 50~60℃ and perform column binding.
- dissolve them by adding more than 5 times of UB bu er for puri cation at high percentage agarose gels.
5. Please use the kit prior to the expiration date.
6. Isolation e ciency can be increased when using EB bu er after pre-heating treatment for 10mins at 50℃ during DNA elution step.
(Especially, in case of long fragment)
7. When washing using NWB without ethanol, extraction time can be shortened by skipping column-idle step.
Product Information
HiGene™ Gel & PCR Purification System [GP104-100]
▶Contents
UB
EB
Help B
NWB
WB Bottle
Spin Column & Collection Tube
Reference
1. WIN, Theint Theint, et al. Monitoring the microbial community shift throughout the shock changes of hydraulic retention time in an anaerobic moving bed mem-
brane bioreactor. Bioresource technology, 2016, 202: 125-132
2. LEE, Seung-Yeol, et al. Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR. The plant pathology
journal, 2014, 30.4: 445.
Maker Cat.No. Product Size
GP104-100 HiGene™ Gel & PCR Puri cation System 100 prep
BIOFACT
GP104-200 HiGene™ Gel & PCR Puri cation System 200 prep
It is available to purchase individually in case of Spin Column & Collection Tube separately. Please contact us for more details.
195
BIOFACT CATALOGUE
Ligase &
Electrophoresis Reagent
Beyond the PCR Technology, BIOFACT Promises the Progress for Your Research.
BIOFACT Provides You the Most Innovative High-Quality Reagents and Lab Supplies
which Lead to Success of Your Tomorrow.
Ligase
T4 Ligase
BioFACT™ T4 DNA Ligase 198
Electrophoresis Reagent
Agarose
gel-rose™ Agarose (Molecular Biology Grade) 199
DNA / RNA Staining Solution
EcoDye™ Nucleic Acid Staining Solution 201
DNA Ladder & 6X Loading Dye
BioFACT™ 1kb Plus DNA Ladder 204
BioFACT™ 100 bp Plus DNA Ladder 204
BioFACT™ 6X Loading Dye 204
Protein Marker
BioFACT™ Triple Color Protein Marker (10 ~ 180 kDa) 207
BioFACT™ Triple Color Protein Marker (10 ~ 245 kDa) 207
Pre-made Bu er
HiGene™ Pre-made bu er 210
BioFACT™ T4 DNA Ligase
Suitable Enzyme for All Common DNA Ligations
BioFACT™ T4 DNA ligase, widely used in both DNA repair and DNA replication, is isolated from a recombinant E.coli strain and
catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA
or RNA. It can join blunt-end and cohesive-end termini (Including sticky, ends for TA Cloning) as well as repair single stranded
nicks in duplex DNA, RNA, or DNA/RNA hybrids.
Feature
• Source : Bacteriophage T4
Application
• Joining of double-stranded DNA with cohesive or blunt end • Inversed PCR product
• Joining of linker or adaptors to blunt-ended DNA • Sub-cloning
• Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids • Cloning of restricted DNA fragments into a plasmid
Product Information
BioFACT™ T4 DNA Ligase [DL101-40k]
▶Contents
T4 DNA Ligase (400 U/㎕),
10X Ligase Bu er
Maker Cat.No. Product Size
BIOFACT DL101-40k BioFACT™ T4 DNA Ligase 40,000 U
198 BIOFACT CATALOGUE
Ligase & Electrophoresis Reagent
gel-rose™ Agarose
Perfect Agarose for Nucleic Acid Applications
gel-rose™ Agarose is suitable for routine analysis of nucleic acids by gel electrophoresis and blotting. It can be available to
use for multi-band electrophoresis applications which need more than 3% agarose gels.
gel-rose™ Agarose o ers accurate analysis through its high purity and clarity.
Feature
• Gel Strength (1% gel) >1200 g/㎠ • EEO Max. 0.13
• Gelling temperature (1.5% gel) 36℃±1.5℃ • Sulfate < 0.15%
• Melting temperature (1.5% gel) 90℃ • DNase, RNase free / Molecular Biology grade
Application
• Electrophoresis of DNA / RNA • Northern, southern blotting of DNA / RNA
• Immuno electrophoresis
Product Information
gel-rose™ Agarose [AG113-100] gel-rose™ Agarose [AG113-500]
▶Contents ▶Contents
gel-rose™ Agarose (powder) [100g] gel-rose™ Agarose (powder) [250g x 2ea]
(+ Spoon)
Maker Cat.No. Product Size
AG113-100 gel-rose™ Agarose 100 g
BIOFACT
AG113-500 gel-rose™ Agarose (with spoon) 500g (250g x 2ea)
199
BIOFACT CATALOGUE
- Comparison of over ow test when making 3% agarose gel between gel-rose™ Agarose and other companies' agaroses
(A) (B) (C)
Over ow has been showed in case of agaroses of
company A and company B
BIOFACT gel-rose™ Company A Company B
QC DATA
- Comparison of cloning e ciency on various agarose purity between gel-rose™ Agarose and other companies' agaroses
Cloning and colony PCR were performed to check target insert DNA after gel extraction step of PCR product using various agaroses.
< PCR Product 500 bp > < PCR Product 1.5kb >
(A) Agarose of BIOFACT gel-rose™ (A) Agarose of BIOFACT gel-rose™
(B) Agarose of company A (B) Agarose of company A
(C) Agarose of company B (C) Agarose of company B
결과 : Gel extraction에 사용되는 Agarose의 purity가 Cloning 효율에 영향을 미치는 것을 확인
- Conclusion: It has been con rmed that purity of agarose used in gel extraction step a ected the e ciency of cloning.
500 bp 1.5 kb 평균
500 bp 1.5 kb Average
BIOFACT
13 / 15
BIOFACT 13 / 15 (ea) 10/ 15 (ea) 10 / 15 76% 76 %
10 / 15
Company A 10 / 15 (ea) 6 / 15 (ea) 6 / 15 53% 53 %
A 사
Company B 9 / 15 (ea) 3 / 15 (ea) 3 /15 40% 40 %
9 / 15
B 사
200 BIOFACT CATALOGUE
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