maintenance and
continuity of
life
By Shahaznani
ACKNOWLEDGMENT
This book is recommended to those who are looking for an easy
way to master chapter one biology form 5 (Microorganisms) as
well as enhance student's learning as a whole. I would especially
like to thank my dear lecturer, Madam Siti Fairuz, for helping me
to complete my precious short book.
The publication of this lesson book involves cooperation from
various parties. Thank you to everyone who helped me in the
publication of this book.
Canva: The Medium for Graphic and Design
The author : Shahaznani Binti Sahran
First published 2022
Published for
School of Science (SCE551)
Collaboration with Anyflip.com (online publisher)
UiTM Cawangan Selangor Kampus Puncak Alam Bandar Puncak
Alam, 42300 Puncak Alam, Selangor
Layout and Typesetting
Canva.com
preface
This lesson book was created based on the biology textbook KSSM
form 5 and the Dokumen Standard Kurikulum Pentaksiran (DSKP)
provided by the Ministry of Education, Malaysia. This book aims to
help students understand and mastering Chapter 1:
Microorganisms required in theme 1: maintenance and continuity of
life. It is guide by the concepts of Scientific Skills, Science Process
Skills, Higher Order Thinking Skills, 21st Century Skills and the
STEM teaching and learning approach. The objective of this
textbook is to develop well-rounded pupils and to equip them with
the skills needed for the 21st century.
Learning SCIENCE
outcome CORNER
List the learning
Additional
outcome for an
information
overview for student
Summative
exercise
Provide questions to
test student's
understanding
TABLE OF CONTENTS
1.1 World of Microorganisms
i. Classification of Microorganisms 1-7
8
ii. Factor Which Affects to Th Growth Of
Microorganism 9-10
1.2 Useful microorganisms
i. Applications of useful microorganisms
ii. Potential Use of Microorganisms in 11
Biotechnology and Sustainability of the
Environmental
1.3 Prevention and Treatment
of Diseases by Microorganisms
i. Aseptic Techniques 12
ii. Methods of treating infections diseases 13
Mindmap 14
Exercise 15
CHAPTER 1
Microorganisms
1
1.1 World of
Microorganisms
Learning Microorganisms are
tiny organisms that can
outcome
only be seen under
At the end of subtopic 1.1,
microscope
student should be able
Describes the
classification of
microorganisms
Explain the factors
that influence microbial
growth
Classification
of Microorganims
Bacteria Algae Protozoa
Fungi Viruses 2
Figure 1.1 Classification of microorganims
Bacteria
Unicellular microorganisms
Does not have membranous
organelles and nuclei
Size Figure 1.2 General structure
of bacteria
0.2 μm to 10μm (1μm = 0,001mm)
Nutrition
SHAPE
Autotrophic
Coccus Coccobacillus Some bacteria have chlorophyll and
are to make their own food by
Spirillum photosynthesis. Some bacteria
synthesis food through
Vibrio Bacillus chemosynthesis using chemic energy
from oxidising chemicals such as
Spirochete nitrite
Figure 1.3 Different shapes Saprophytic
of bacteria Feed on dead and decaying organisms
REPRODUCTION Parasitic
Obtain food from living organisms
Asexual
Binary fission HABITAT
Air
Water
Food
Soil
On or inside other organisms
Figure 1.4 Binary Fission Sexual 3
Conjugation
Spores formation
Figure 1.5 Conjugation
Fungi
Figure 1.6 Structure of fungal cell
Some fungi are unicellular, like yeast
Some fungi are multicellular, like mucor
Size
10μm -100μm
Figure 1.7 Yeast SHAPE
Spheres Figure 1.7 Mucor
Filamentous
HABITAT
Normally live in places which contain a lot of decaying
matter, animal skin and food.
Fungi also grow well in dark and moist places
Nutrition
Some fungi are saprophytes (obtain nutrient from dead
and decaying organism)
Some fungi are parasites (obtain nutriens from their
hosts)
REPRODUCTION
Asexual Sexual 4
Budding in yeast Conjugation
Formation of
- Occurs when there is
spore in mucor a lack of water and
food
PROTOZOA
Figure 1.8 Structure of Amoeba Figure 1.9 Structure of Paramecium
Unicellular organisms that contains
Shape
nucleus, cytoplasm and plasma membrane
Spherical
Size Coil
Irregular
5um - 250um
HABITAT NUTRITION
Paramecium sp. lives in fresh
Euglena sp. carries out
water photosynthesis.
Amoeba sp. lives in the moist
Plasmodium sp. is parasites.
soil, fresh water, seawater
Amoeba sp. obtains nutrients by
and its host. phagocytosis. Used pseudopodia
or "false feet" to move and engulf
REPRODUCTION food during phagocytosis
Asexual SCIENCE
Amoeba sp. , binary fission. CORNER
Figure 2.0 Binary fission in Amoeba
Sexual
Paramecium sp. , conjugation.
Amoeba movement with pseudopodia
5
under a microscope
Figure 2.1 Conjugation in Paramecium
vIRUSES Bacteriophage
The smallest microorganims
No cell organisatiom
Do not contain cytoplasm, plasma membrane or
nucleus
Can crystallize
Reproduce inside living cell
Contain nucleic acids (DNA or RNA) and covered
in protein sheath
Ebola virus
Tobacco mosaic virus Figure 2.2 Example of viruses
Size REPRODUCTION
o.2um to 0.4um Live in living host cell, form crystal outside a
living cell
Shape
Spherical, (influenza virus)
Helical (mosaic virus)
Polyhedral (adenovirus)
Complex ( Helical + polyhedral,
bacteriophage)
NUTRITION Figure 2.3 Virus replication
Do not need food
HABITAT
Live in host cells
From crystals outside a living
cell
SCIENCE The virus that causes COVID-19, enters your body through
CORNER your mouth, nose or eyes. It then travels to the back of
your nasal passages and mucous membrane in the back of
your throat. It attaches to cells there, begins to multiply
6
and moves into lung tissue. From there, the virus can spread
to other body tissues. Figure 2.4 Corona
Virus
ALGAE
Chlamydomonas sp Figure 2.5 Example of Algae Spirogyra sp
SIZE SHAPE
1μm to 100 μm Unicellular Chlamydomonas sp
Multicellular Spirogyra sp have
Habitat chloroplast which contain
chlorophyll
Fresh water, salt water, moist
NUTRITION
soil and tree barks that are
exposed to sunlight
REPRODUCTION Most algae are green in colour
because of the presence of
Reproduce asexually by binary
chlorophyll that enable to carry
fission sexually by conjugation photosynthesis to produce their
Spirogyra sp. reproduce sexually
own food
by conjugation
Summative
exercise
Why are algae not
found at the bottom
of the ocean ?
Clue 7
* have chloroplast
Factor Which Affects to The
Growth Of Microorganism
Light Moisture
•Microorganisms with
Microorganisms need water for
chloroplasts such as algae
biochemical reactions and growth
can grow well in well-lit
environments The condition is best when relative
humidity is between 25% to 40%
•Most other microorganisms
prefer darker Environments Most microorganisms will die in
dry conditions, but some will
Nutrients produce spores
Temperature
•Microorganisms apart from
viruses, require nutrients
The optimum temperature for microbial
•Autotrophs synthesize their own
growth is mostly between 35°C to 40
food °C
•Parasites obtain food from the
Microorganisms become less active at
host cell low temperatures (<5 °C)
•Saprophytes obtain food from
Microorganisms will die at high
dead or decaying matters temperatures (>60 °C)
Spores can withstand temperatures
as high as 120 °C
pH value 8
Most of microorganisms
prefer to live in neutral
medium (pH = 7)
In very acidic (pH = 1 to 3)
and very alkaline (pH = 12 to
14) conditions, most of
microorganisms will be killed
1.2 Useful
microorganisms
Learning Industry Agriculture
Medicine
outcome Applications of
useful
At the end of subtopic 1.2,
student should be able microorganisms
Explain the application
of useful
microorganisms
State the potential
use of microorganisms
in biotechnology and
sustainability of the
environmental
INDUSTRY
FOOD & BEVERAGES
Yeast
Bacteria such as
is added to dough
Lactobacillus
added into raw milk produces
- making bread lactic acid - making yoghurt
Yeast LEATHER INDUSTRY
Alcoholic beverages Bifidobacteria
-Sugar fermentation in
grapes to produce wine Microbial enzyme is used to
break down soft tissue on the
skin of animals. The skin is then
9
used to produce leather
products.
AGRICULTURE
Increase and improve the Convert organic substances into inorganic
soil fertility compounds
Nitrogen-fixing bacteria Nitrifying bacteria
- Rhizobium -Nitrobacter sp
-Nitrosomonas sp
Figure 2.6 Rhizobium in root nodule Figure 2.7 Example
of Nitrifying bacteria
Denitrifying bacteria
Bacteria of decay
- decaying organic matter of plants
-Pseudomonas
and animals
Figure 2.8 Pseudomonas
MEDICINE
ANTIBIOTICS VACCINE
Such as penicillin Are produced by weakened
harmful microorganisms
To kill bacteria caused by
various diseases Vaccines can help protect
against certain diseases by
imitating an infection
10
Potential Use of Microorganisms in
Biotechnology and Sustainability of
the Enviromental
Biotechnology is the technology which uses living organisms or biological processes
to produce beneficial products.
The genetic structure of bacteria is changed through genetic engineering to
produce bacteria that give benefits to us.
Enzyme production
• Eco enzymes also have cleaning properties due to the alcohol or acetic acid
produced in the solution. Alcohol (ethanol) or acetic acid is produced by the
metabolic processes of bacteria that exist naturally on the fruit peel or
vegetable scraps.
• Eco enzyme liquids can be used as a eco- friendly cleaning agent comparable
to the cleaning power of commercial detergents.
Figure 2.9 Eco enzyme cleaning solution Figure 3.0 Chemical cleaning substance
Lactobacillus sp. Bacterial Serum
Lactobacillus sp. bacterial serum is used to treat wastewater and sludge
in drainage systems
Uses of Lactobacillus
sp. bacterial serum
Removes Treats Makes Improves
odour Compost the fishing
sewage industry
Figure 3.1 Lactobacillus sp. Bacterial Facilitates
Serum 11
animal
digestion
1.3 Prevention and Treatment
of Diseases by Microorganisms
Learning Aseptic Techniques
in controlling the spread of the
outcome
microorganisms
At the end of subtopic 1.3,
student should be able 1. Sterilization
State the Aseptic
techniques and the
The process of destroying or
methods of treating
eliminating pathogens from an object,
infections diseases
food or environment.
2. Boiling 3. Use of Antiseptics
Boiling water can kill almost all
Chemicals used to kill or inhibit the growth
microorganisms, except for those with
of microorganisms on skin
thick- walled spores.
particularly on wounds to prevent the
growth of pathogens.
Autoclave is a type of high-pressure pot
that allows the water inside to be
Some Examples of Antiseptics include iodine
compressed under high pressures and
heated up to 120°C to kill microorganisms
solution, hydrogen peroxide, ethanol,
acriflavine and potassium permanganate
and spores.
solution.
Figure 3.2 Example of
antiseptics
Figure 3.3 Example of 4. Use of Disinfectants
disinfectants
Suitable to sterilize walls, floors,
12
blankets, bedsheets, toilet bowls and
medical tools
Some examples of disinfectants are
iodine solution and chlorine gas,
Methods of treating
infections diseases
Antibiotic
Used to kill or inhibit the growth of bacteria
Taken orally or via injection
Example:
Streptomycin (produced by Streptomyces griseus) is used to treat
tuberculosis
Penicillin (produced by Penicillium chrysogenum) is used to treat syphilis
Antifungal
Used to treat fungal infections
Available in powder, lotion or gel form
Example:
To treat athlete's foot
To treat skin conditions like a yeast or ringworm infection
Antiviral
Used to inhibit virus growth
Taken orally, via injection or applied on the affected area
Example:
To treat shingles
Summative SCIENCE
exercise CORNER
How do antiviral and
Figure 3.4
vaccine act on virus? Athlete's foot
Athlete's foot (tinea pedis) is a
13
fungal skin infection that usually
begins between the toes.
MINDMAP
BACTERIA PROTOZOA LIGHT NUTRITION
MICROORGANISMS FACTOR
VIRUSES MOISTURE
FUNGI PH VALUE
ALGAE TEMPERATURE
USEFUL
MICROORGANISMS
INDUSTRY AGRICULTURE MEDICINE
Antibiotics
Sterilization
Antiseptics Aseptic Boiling
Techniques
Treatment
infections Antifungal
Disinfectants
Antiviral
14
Total score:
TEST TIME
/20
1 What is microorganisms and state the five group of microorganisms
/6
2 a) What is Y ? /4
b) State the function of Y ?
3 State five factor that affect the growth of
/5
microorganisms ?
4 State one example of useful microorganisms in each of the
following
a) Medicine c) Industry
b) Agriculture /3
5 Explain the use of antibiotics /2
15
References
Biology LibreTexts (Jan 4, 2021). Types of Microorganisms. Retrieved from
https://bio.libretexts.org/Bookshe
lves/Microbiology/Book%3A_Micr
obiology_(Boundless)/1%3A_Introduction_to_Microbiology/1.2%3A_
Microbes_and_the_World/1.2A_Types_of_Microorganisms#:~:text=K
ey%20Points,mean%20of%20locomotion%2C%20and%20reproduct
ion.
F5 Chapter 1 Microorganisms and Their Effects on Living Things, Anyflip.
Retrieved from https://anyflip.com/rlwt/vieg
BiologyDiscussion. Top 10 Uses of Microorganisms. Retrieved from
https://www.biologydiscussion.com/microorganisms/top-10-uses-
of-microorganisms-zoology/34263
Airtek (June 15, 2017). Factors Affecting Microbial Growth. Retrieved from
https://www.airtekenv.com/2017
/06/15/factors-affecting-microbial-
growth/#:~:text=Warmth%2C%20moisture%2C%20pH%20levels%20and
,the%20biggest%20overall%20issues%20present.
National Library of Medicine, (2010) What You Need to Know About
Infectious Disease. Retrieved from
https://www.ncbi.nlm.nih.gov/books/NBK209704/
Science Form 5 KSSM, Anyflip. Retrieved from
https://online.fliphtml5.com/knkiy/jrfb/#p=1
Pandai. Chapter 1: Microorganisms, Quick notes. Retrieved from
https://question.pandai.org/note/chapter/kssm-f5-sc-01
16