Abstracts

BIO-O-08 8

Microplastics Composition and Abundance in the Coastal Chonburi province

Watcharabhorn Sungkaphan1,* and Vichaya Gunbua2
1 Graduate Student of Environmental Science Program, Faculty of Science, Burapha University, Chonburi, 20131
2 Department of Aquatic Science, Faculty of Science, Burapha University, Chonburi, 20131
* Corresponding author email: [email protected]

This study investigated the abundance and composition of microplastic debris in Chonburi province, eastern of
Thailand. The water and sediment samples collected from 7 stations at Chonburi Bay in March, June,
September and December 2020. The manta net and a submerged pump sampler were used for water and
sediment sampling, respectively. There were 6 compositions of microplastic were observed (filament, fragment,
foam, film, granule and pellet). The filament exhibited the highest abundance in both water and sediment
samples. In terms of color, the sampled plastics were red, black, white, green, blue, brown and yellow, while
microplastic in the size range 10 – 1,000 µm were found in water and sediment samples. In comparison of 3
sampling methods, the average densities of 15.63±8.22 and 40.96±29.14 particles/m3 were found in manta net
and submerged pump, respectively. While sediment method show the average densities of 119.3±93. 11
particles/kg. Based on the sampling site (station) and the sampling frequency of the present study, there were
significant differences (p < 0.05) between the abundance and composition of microplastic debris in the coastal
area of Chonburi province.
Keywords: Chonburi province, Microplastics, Manta net, Submerged pump

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วจิ ัย ครงั้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
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BIO-O-09 9

Greenhouse Gas Emissions from Travel by Rowing and Canoeing Thailand Championships

Wanitcha Kaewsuwan 1, Tanormsak Senakham 2 and Supika Vanitchung 1*
1 Faculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Bangkok, 10110
2 Faculty of Physical Education, Srinakharinwirot University, Bangkok, 10110
* Corresponding author email: [email protected]

Thailand rowing championship including kayak-canoe, rowing, and dragon boat. It is an interesting activity to
study greenhouse gas emissions This research focuses on greenhouse gas emission assessment.
The assessment of greenhouse gas emission from traveling to participated in The Rowing Boat Race of
Thailand 2019 (field 3) between 19th - 22th December, 2019 at Bhumibol Dam. The traveling activities data
to participated in that tournament was collected by using the low carbon water sports tourism assessment
interview with low carbon questionaries. Total participant are 27 teams; 934 people. Total greenhouse gas
emissions from traveling to participated was found as 14,103.58 kg CO2 eq and average as 15.10 kg CO2
eq/person. The most used fuel is diesel, emission greenhouse gas as 14,067.32 kg CO2 eq. The bus had
the most emission as 7,806.19 kg CO2 eq. However, a low volume of greenhouse gas emission from sedan
and bus is 1.01 and 11.81 kg CO2 eq/person respectively, compared with other type due it can transport
passengers in large quantities. The main cause is the amount of fuel used and the type of fuel that make the
emission lower when compared with greenhouse gas emission per person.
Keywords: Greenhouse gas, Rowing, Tak Province, Car type, Traveling

การประชุมวชิ าการระดับชาติ วทิ ยาศาสตรว์ ิจัย คร้งั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

BIO-O-10 10

Mangrove Plants Community at Learning Center of Bang Pakong Power Plant,
Chachoengsao Province

Benchawon Chiwapreecha1,*and Vichaya Gunbua 2
1 Department of Biology, Faculty of Science, Burapha University, Chonburi, 20131
2 Department of Aquatic Science, Faculty of Science, Burapha University, Chonburi, 20131
 Corresponding author email: [email protected]

Study of mangrove plants at Learning Center of Bang Pakong Power Plant, Chachoengsao Province, was
made during December 2018 to July 2019. The objectives were to study species diversity and plants
composition in mangrove forest. The two permanent plots were determined along the coast of Bang Nang canal
(A) and deep into the interior of mangrove (B). Twenty-three species belonging to 15 families and 16 genera
were enumerated. Plant community in plot A had 1.495 diversity index and tree density were 198 individual/Rai.
Xylocarpus granatum J. Koenig was a dominant species which the Important Value Index was 160.06. While,
plot B had 1.474 diversity index and tree density was 464 individual/Rai. Rhizophora mucronata Lam. was
dominant which the Important Value Index was 158.21. A proportion of similarity index was 41.38 %. The
results indicated that the Learning Center of Bang Pakong Power Plant had a quite perfect mangrove forest.
The succeeded mangrove species from canal coast in restoration area was rather moderately. However,
suitable planting space and environment were important for restoration forest.
Keywords : riverine forests, Bang Pakong river estuary, Importance Value Index

การประชุมวชิ าการระดับชาติ วิทยาศาสตร์วิจัย ครงั้ ที่ 12
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BIO-O-11 11

Study of Ethnobotany for conservation and utilization in Phayao Province

Sitthisak Pinmongkolkul 1* and Thatchakorn Khamkhunmuang 2
1 Department of Biology, School of Science, University of Phayao 56000
2 Department of Biology, Faculty of Science, Chiang Mai University 50200
 Corresponding author email: *[email protected]

As the diversity of plants in each locality, the villagers have used these plants as food and medicines in various
natural ways. The variety of plant species depends on different terrain. Therefore, determines the distribution
of species. Therefore, this study aims to study native botanical varieties (Ethnobotany) for conservation and
utilization sustainability in Phayao Province for preparing a database of plant botanicals. The results show that
classification of potential plants there are 2 topics and 4 dimensions. The tourism topic there are beauty and
nature dimension. Tagetes erecta L. (Marigold) has essential to landscaping, farming or field and sell of original
marigold varieties dimension of culture. Senegalia rugata (Lam.) Britton & Rose, development as products of
beliefs and rituals, such as souvenirs, dimension of food, restaurants, souvenirs and products from vegetables,
savory taste and smell. Houttuynia cordata Thunb. (Khawtong) developed into various desserts to reduce the
smell and bitter taste, such as cakes, cookies or various bakery products, etc. that everyone can access in the
product. Zanthoxylum limonella (Dennst.) Alston has tourism dimension of food. Alston dried fruit is packaged
to create a spice product with a characteristic odor and flavor. The health topic, Andrographis paniculata (Burm.
f.) Wall. ex Nees tablets the potential for herbal medicines dimension in the National List of Major Medicines.
Thunbergia laurifolia Lindl. vaguely has truffle leaves can be shredded. Including, tea vaguely helps detoxify
the body as well. Cissampelos pareira L. var. hirsuta (Buch. ex DC.) Forman. (kruekmanoi), has properties as
a cold remedy for heat to neutralize fever. Local plants deserve to be conserved and developed into a tourist
attraction. The dimension of ecotourism and natural beauty. The dimension of plant belief, religion and tradition.
The food dimension which will lead to the promotion and sustainable use.

Keywords: Conservation, Utilization, Ethnobotany, Phayao

การประชมุ วิชาการระดับชาติ วิทยาศาสตร์วิจัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
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BIO-O-12 12

Anatomical and histological studies on the digestive system of the giant African land snail
Lissachatina fulica (Pulmonata: Achatinidae)

Kris Srisilapaudom1 Siravit Sinprasertrat1 and Thanit Siriboon1,*
1 Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
 Corresponding author email: [email protected]

Lissachatina fulica (Bowdich, 1822), also known as giant African land snail, is a crop pest found as an invasive
alien species widely distributed across the tropical zone including Africa, Australia, and Southeast Asia. They
are pulmonates of which the digestive system curls around their spiral shell. The aim of this research is to
study the structure of digestive system in giant African land snail. The digestive organs were histologically
compared to improve insight into physiology of digestive system. The results showed that anatomical study
could not clearly distinguish between esophagus and crop. Thus, a histological method was used to examine
the organs. The tissue of organs involved in the digestive tract is divided into 4 layers: epithelial layer,
connective tissue layer, muscular layer and covering layer. In case of accessory organs such as the salivary
gland, the acini-lined tissue associated with serous and mucous formation was found. Moreover, the digestive
gland contains small lobes which are composed of the arrangement of many types of cells functioning in the
production of digestive enzymes in the intestine.
Keywords: giant African land snail, digestive system, anatomy, histology, digestive gland

การประชมุ วิชาการระดับชาติ วทิ ยาศาสตร์วจิ ัย ครัง้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
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BIO-O-13 13

Anatomical and histological studies on the nervous system of the giant African snail
Lissachatina fulica (Pulmonata: Achatinidae)

Siravit Sinprasertrat 1 Kris Srisilapaudom 1 Thanit Siriboon 1,*
1Department of Biology, Faculty of Science, Srinakharinwirot University
Corresponding author, email: [email protected]

Nervous system of The Giant African Land Snail, Lissachatina fulica (Bowdich, 1822) consists of 4 ganglia
which are buccal ganglia, cerebral ganglia, pedal ganglia and tentacular ganglia. Each ganglion is associated
with the branching network of nerves that support body functions in the snail. This study could reveal insights
into buccal ganglia, cerebral ganglia and pedal ganglia with anatomical method. However, the method could
not be applied to tentacular ganglia which were therefore studied with histological method instead. The
results showed that a number of neurons were found in each ganglion. The neurons are polyhedral cells with
varying shape of nucleus. Moreover, large neurons called giant ganglionic cell were also found.
Keywords: nervous system, anatomy, histology, African land snail

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

BIO-O-14 14

Detection of methicillin-resistant Staphylococcus aureus and mecA gene among students
in Science Classrooms in University-Affiliated School Project, Piboonbumpen
Demonstration School

Jarurumpa Buthsri 1,*, Pitchaya Komasathid1, Piyanoot Jaihan1 and Kanjana Hrimpeng2
1 Science Classrooms in University-Affiliated School Project, Piboonbumpen Demonstration School, Chonburi, Thailand, 20131
2 Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand, 20131
* Corresponding author email: [email protected]

The purpose of this study was to detect methicillin-resistant Staphylococcus aureus (MRSA) and mecA gene
from students of Piboonbumpen demonstration school in Chonburi province using antibiotic susceptibility test
and molecular techniques. In this study, 95 isolates were investigated from swab test (nasal & nail : 33, skin :
29). The samples were identified as S. aureus by Gram staining and coagulase test. The results showed that
a total of 7 S. aureus of all 95 isolates (7.4%) were obtained and the most common site of them was nasal
and nail, around 42.86% (3 of 7 isolates). Antibiotic susceptibility test using disk diffusion method revealed that
7 isolates were susceptible to cefoxitin, which suggested that all isolates were methicillin-susceptible
Staphylococcus aureus (MSSA). However, the detection of mecA gene with PCR test presented 2 isolates
were MRSA. In conclusion, MRSA were contained in students of Science Classrooms in University-Affiliated
School Project, Piboonbumpen Demonstration School.
Keywords: Staphylococcus aureus, mecA, MRSA, Methicillin-resistant Staphylococcus aureus

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลัยนเรศวร

BIO-O-15 15

The global database of P. falciparum 25-KDa antigen for developing transmission blocking
malaria vaccine

Pornpawee Sookpongthai1 and Sittiporn Pattaradilokrat1,*
1 Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand, 10330
* Corresponding author email: [email protected]

Transmission blocking vaccines (TBV) against the malaria are considered an important tool for controlling the
spread of the drug resistant parasite and eradication of the disease. One of the key TBV components is P.
falciparum 25-KDa antigen (Pfs25), the surface protein Plasmodium falciparum required for the malaria
development in the mosquito vector. Although Pfs25 vaccines are readily developed using a lab strain (3D7)
of P. falciparum and now tested in clinical fields, the extent of the diversity of the gene in endemic populations
remains largely unknown. To address this, the Pfs25 sequences of P. falciparum in Thailand and from the
GenBank and PlasmoDB databases were collected to generate the first global database of the 307 Pfs25
sequences. The analysis of the sequences in the database identified 11 haplotypes, including 9 novel
haplotypes. Of these, H1 (the vaccine type) and H2 were the two main haplotypes that represented >90% of
different P. falciparum populations. According to the haplotype network analysis, H1 and H2 may give rise to
all other minor haplotypes. Interestingly, H1 and H2 simply differed by a single mutation (392 GGA/GCA) that
resulted in a non-synonymous amino acid change from glycine to alanine. The understanding of the molecular
variation could benefit the prediction of the vaccine efficacy in filed study. In summary, the global database of
Pfs25 could be useful for the malaria epidemiology and vaccine studies and could aid in monitoring of the
vaccine efficacy.
Keywords: Diversity, Haplotype, Pfs25

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-O-16 16

Detection of Hb Constant Spring, Hb Pakse and Hb Quong Sze by real-time PCR and high
resolution melting analysis

Bunnareth Ny 1, Angkhana Ruenros 1, Thanyasiri Patanompee 1, Arunee Pingyod 2, Khwanruedee Mahingsa 2, Wanwipa Bumrungpakdee
2, Torpong Sanguansermsri 2 and Narutchala Suwannakhon 1,*
1 Discipline of Biology, School of Science, University of Phayao, Phayao, 56000
2 Thalassaemia Unit, University of Phayao, Phayao 56000
* Corresponding author email: [email protected]

Alpha thalassemia is one of the most common inherited anemia disorders in Thailand with prevalence at 30 to
40%. Non-deletional alpha thalassemia 2 gives rise to severe Hb H (--/-αT) disease. Thus, the rapid and
accurate method for detection of non-deletional alpha thalassemia 2 was important for thalassemia treatment
and prevention. The aim of this study was to develop HRM method for detection of non-deletional alpha
thalassemia 2 including Hb CS, Hb PS and Hb QS. Two HRM primers, which specified for alpha thalassemia
2, were used to identify three non-deletional alpha-thalassemia 2, and the optimized temperature was performed
by gradient PCR. Next, real-time PCR and HRM analysis were examined with control DNA, that was determined
by DNA sequencing. After that, we examined 40 DNA samples from Thalassaemia Unit, University of Phayao.
The real-time PCR and HRM analysis could be able to identify control DNA including Hb CS trait, Hb H
CS, homozygous Hb CS, Hb PS trait and Hb QS trait from negative DNA. 30 of 40 samples were negative,
3 samples were Hb CS trait while, PCR product was not detected in 7 samples, that were the alpha thalassemia
2 with 3.7 deletion. In conclusion, the real-time PCR and HRM method appears to be an accurate and sensitive
method for the rapid screening and identification of Hb CS, Hb PS and Hb QS. We suggest that this method
should be used in co-diagnosis of the deletional alpha thalassemia.

Keywords: Alpha thalassemia, Non-deletional alpha thalassemia, High resolution melting analysis,
Hemoglobin

การประชุมวชิ าการระดับชาติ วิทยาศาสตรว์ ิจัย ครัง้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลัยนเรศวร

BIO-O-17 17

Molecular isolation and characterization of the tumor necrosis factor receptor-associated
factor 6 (TRAF6) gene from Macrobrachium rosenbergii

Chanitcha Choolert1,*, Phongthana Pasookhush1, Akapon Vaniksampanna1 and Parin Chaivisuthangkura1
1 Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Corresponding author’s email: [email protected]

TRAF6 (tumor necrosis factor receptor-associated factor 6) is a crucial adapter molecule in Toll signaling, which
activates downstream invertebrate innate immunity cascades. In this study, the novel TRAF6 gene (named as
MrTRAF6) of Macrobrachium rosenbergii, giant freshwater prawn, was successfully isolated and characterized.
MrTRAF6 full-length cDNA was 2114 bp long, with and open reading frame (ORF) encoding a protein of 564
amino acid residues. The predicted amino acid sequence of MrTRAF6 had a conserved TRAF family motif
including two RING type Zinc fingers and a conserved C-terminal meprin and TRAF homology (MATH) domain
with no signal peptide. The putative amino MrTRAF6 and other TRAF6s had 45–99% identity. Phylogenetic
analysis classified MrTRAF6 as a true TRAF6 ortholog. Real-time RT-PCR was used to assess MrTRAF6
mRNA expression in different tissues. Gill, heart, hepatopancreas, hemocyte, muscle, stomach, and intestine
tissues all had constitutive expression of the mRNA transcripts. MrTRAF6 transcription was clearly induced in
the gill, hepatopancrease, hemocyte, and muscle after immune challenge with Aeromonas hydrophila. These
findings suggested that MrTRAF6 can play a role in innate immunity against bacterial infection.
Keywords: Macrobrachium rosenbergii, TRAF6, Gene expression

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วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

BIO-O-18 18

Prolonged Expression of SUC2 Promoter for Enhancement of Recombinant Secretory
Protein Production in Saccharomyces cerevisiae

Poravee Santiarphon1 and Salil Chanroj1,*
1 Department of Biotechnology, Faculty of Science, Burapha University, Chonburi, 20131
* Corresponding author email: [email protected]

The major problem for recombinant secretory protein production in Saccharomyces cerevisiae is the amount
of secreted protein is generally inadequate for further uses. Especially, when SUC2 promoter which controls
the expression of an invertase enzyme is induced by the presence of sucrose, is introduced in the plasmid
construct. Our goal is to prolong the expression regulated by SUC2 promoter for increasing the amount of
recombinant secretory proteins in transgenic yeasts. Results indicated that using SUC2 promoter and its
signal peptide cassette to control the expression of engXCA cloned from Xanthomonas oryzae pv. oryzae
enabled S. cerevisiae to secrete endoglucanase, yet in small quantities. To cope with this problem, we tried
adding glycerol instead of sucrose from the second day of yeast cultivation. As expected, specific activity of
endoglucanase in supernatant of transgenic yeast culture was increased in the third and fifth days of the
experiment when compared to the control. This knowledge is crucial for enhancing capability of recombinant
secretory protein production in S. cerevisiae allowing us to manage the expression of those proteins using
only table sugar and glycerol.
Keywords: Endoglucanase, Sucrose, Glycerol, SUC2 Promoter, Saccharomyces cerevisiae

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BIO-O-19 19

The identification of Vibrio spp. contamination in fresh and preserved seafood using
nucleotide sequence of 16S rRNA gene

Palika Sangtongsittichok1, Romteera Boongor1 and Piyanoot Jaihan2*
1 Piboonbumpen Demonstration School, Burapha University Chon Buri, 20131
2 Science Classrooms in University-Affiliated School Project, Faculty of Science, Burapha University, Chon Buri, 20131
* Corresponding author email: [email protected]

Nowadays, Thai people are eating more preserved fresh seafood which may contaminate from bacteria which
cause of food poisoning. The aims of this study were to compare total microorganism count of fresh and
preserved seafood and to identify pathogenic bacteria that contaminates fresh and preserved seafood by using
nucleotide sequence method. Samples of Shrimp, Blue swimming crab and Bloody cockle at Angsila Pramong
Peunban market were collected. The experiment divided into 8 types includes fresh seafood, 24HR preserved
seafood and 48HR preserved seafood. The total bacteria were counted by standard plate count technique. The
result revealed that total microorganism count of fresh seafood of Shrimp, Blue swimming crab and Bloody
cockle were 6.86 x 104 CFU/g, 1.86 x 105 CFU/g, 3.19 x 106 CFU/g, respectively. Whereas, the preserved
seafood of 24HR were 6.30 x 103 CFU/g, 4.03 x 104 CFU/g and, 8.80 x 104 CFU/g and the preserved seafood
of 48HR were 2.85 x 102 CFU/g, 3.26 x 103 CFU/g and, 1.56 x 104 CFU/g, respectively. The values were within
the standard criteria of the Department of Medical Science (DMS) required for safety. In addition, total bacteria
were tested by thiosulfate citrate bile salts sucrose ( TCBS) agar, observed by gram staining test, and finally
identified by the 16S rRNA gene sequence analysis. The result showed that only eight of isolated bacteria
grew on a selective medium (TCBS agar) were gram negative curved rod. Based on 16S rRNA gene sequence
analysis, these isolates can be divided into 5 clades: clade 1 consisted of 3 isolates of Vibrio parahaemolyticus;
clade 2 contained 1 isolate of V. alginolyticus; clade 3 contained 1 isolate of V. cholerae; clade 4 contained 2
isolates of Aeromonas sp. and clade 5 contained 1 isolates Shewanella sp.

Keywords: nucleotide sequencing, pathogenic bacteria, seafood

การประชมุ วิชาการระดับชาติ วทิ ยาศาสตร์วจิ ัย คร้งั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-O-20 20

Efficiency of Streptococcus agalactiae inhibition of Piper betle L. crude extract and its
application in Oreochromis niloticus culture

Pajaree Jindaroek1,* and Chonnikarn Rattanopat2
1 Paphayompittayakom school, SCiUS – Thaksin university, Phatthalung, Thailand, 93210
2 Paphayompittayakom school, SCiUS – Thaksin university, Phatthalung, Thailand, 93210
* Corresponding author email: [email protected]

The pathogenic bacteria Streptococcus agalactiae is present in Oreochromis niloticus. It is a significant issue
for farmers. This decreases productivity and may be the source of disease outbreaks in the surrounding area.
Farmers prefer use antibiotics in tilapia farming has resulted in antibiotic residue and S. agalactiae resistance.
As a result, the aim of this analysis was to see how effective betel extract was at inhibiting S. agalactiae disease
using two solvents: ethanol and hexane. The paper disc diffusion method was used to measure the inhibitory
efficiency. The crude extract of betel leaves obtained from both solvents at concentrations of 24 48 and 96
mg/mL Ethanolic extract has clear zones were measured at 14.7±0.05, 17.4±0.05, and 21.9±0.09 mm. Hexane
extract has clear zones measured 13.1±0.06, 15.8±0.04, and 19.4±0.07 mm, respectively.
Keywords: Streptococcus agalactiae, Oreochromis niloticus, Piper betle L.

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
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BIO-O-21 21

The simulation of floating debris distributing from the Bangpakong River to Bangsaen
Beach by using computer modeling

Yanisa Sriwiriyarat1,*, Nattakul Ingkaninant1 and Anukul Buranapratheprat2
1 Piboonbumpen Demonstration School, Burapha University, Chonburi, Thailand, 20131
2 Department of Aquatic Science, Faculty of Science, Burapha University, Chonburi, Thailand, 20131
* Corresponding author’s email: [email protected]

Recently Thailand is ranked as the 7th country for polluting the oceans with mismanaged plastic waste. The
Bangpakong River, located in the eastern part of the upper Gulf of Thailand, runs through 11 provinces carrying
debris along the way to the sea affecting the upper gulf region including Bangsaen Beach. Therefore, this study
aims to simulate patterns and directions of the floating debris which are non-specific size objects (passive
tracer) flowing from the Bangpakong River to Bangsaen Beach in 2 cases conducted: debris flowing
independently(case 1) and debris that stuck to the sea bottom or along the coastline(case 2) using Princeton
Ocean Model(POM) for current simulation with oceanic bathymetry, wind, tide, tidal influence, and salinity as
model input for 4 representative months including March, July, October and December. The outputs were
plotted using Ocean Data View software. The results showed that in March, the floating contaminants remained
near Bangsaen Beach but did not affect the coastal zone for both cases while in July they moved closer to the
coastal area caused by the southwest wind. The contaminant moved to the coastal zone in October and stuck
along Bangsaen Beach for both cases in December. Those results demonstrate that seasonal variations in
directions and speed of wind and current are the key factors to control floating debris’ distribution. The
contaminants mostly affected Bangsaen coastline in December and attached on the seafloor in the upper gulf
in October. And most of them remained in the upper gulf until the end of March.

Keywords: Princton ocean model, Contaminant, Bangpakong river, Bangsaen beach, Gulf of Thailand

การประชุมวิชาการระดับชาติ วทิ ยาศาสตรว์ จิ ัย ครง้ั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

BIO-O-22 22

3D model of cardiovascular disease for human anatomy learning

Nattawat Natee1, Nutnaree Opala1 and Chatchawal Wongchai2,*
1 Demonstration School University of Phayao, Phayao, Thailand, 56000
2 Division Biology, School of Science, University of Phayao, Phayao, Thailand, 56000
* Corresponding author’s email: [email protected]

Currently, the technology for medical instruction model is important for fast learning and well understanding.
3D printer shows high ability to create complex structural organ and tissue of human. In Thailand, there are
many people with coronary heart disease which have different form of blood vessel defect, especially the
elderly. Our research focuses on 3D model of Hyperlipidemia, which is a disease caused by fat deposits in the
arteries, Hemorrhagic Stroke is caused by fragile blood vessels with high blood pressure the arteries rupture,
Acute Aortic dissection is caused by a tear of the vascular layer. Moreover, the blood flow to accumulate under
the vascular layer and erosion indefinitely and A thoracic aortic aneurysm caused by the degeneration of blood
vessels, which cause blood vessels to rupture are created. Our 3D model will promote and support human
anatomy learning class and laboratory framework and provide high efficiency to understand a complex
cardiovascular disease and blood vessel problem.
Keywords: Heart attack, Heart disease, Plastic filament, Plastic model, Stroke

การประชมุ วิชาการระดับชาติ วิทยาศาสตร์วิจัย ครงั้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-O-23 23

Morphogeometric analysis of terrestrial carnivorous snail Oophana mouhoti from
limestone karst of Thailand

Sittichai Chunkhajorn1 and Thanit Siriboon2,*
1 Biology Master Student, Department of Biology, Faculty of Science, Srinakharinwirot University
2 Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Corresponding author email: [email protected]

Oophana mouhoti is a terrestrial carnivorous snail in the family Streptaxidae distributed in limestone karst of
Phetchaburi and Prachuap Khiri Khan Provinces. Intraspecific variation of shell morphological characters was
highly occurred. This study aimed to clarify the relationship between the shell variation and their biogeographic
distribution using landmark-based geometric morphometric analysis and canonical variate analysis (CVA). The
results showed that the shell variation of O. mouhoti was associated with three groups of biogeographic
distribution. Group 1 was distributed in Tenasserim range, while group 2 was distributed in the mountain range
in eastern Phetchaburi. Group 3 was found in Khao Sam Roi Yot range. The results indicated that distribution
areas of O. mouhoti in Phetchaburi and Prachuap Khiri Khan Provinces were significantly related to the shell
variation based on mahalanobis distance (P<0.0001). This finding could be important data for further systematic
research of carnivorous snails in the genus Oophana.
Keywords: Streptaxidae, morphogeometric analysis, carnivorous snail

การประชุมวิชาการระดับชาติ วิทยาศาสตร์วจิ ัย ครั้งท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

BIO-O-24 24

Green peafowl conservation and youth network

Naphat Boonphrom1, Raphiwit Aeumphan1, Witchaphon Chompooming1 and Chatchawal Wongchai2,*
1 Demonstration School University of Phayao, Phayao, Thailand, 56000
2 Division Biology, School of Science, University of Phayao, Phayao, Thailand, 56000
* Corresponding author’s email: [email protected]

Natural resources conservation has been involved in the development of agriculture areas, illegal wildlife trade
and destruction of natural habitat. There is a need to get cooperation from the government, people, and related
organizations to promote the potential for sustainable uses of the area, under the paradigm of the United
Nations to promote agriculture and increase income for the community. One of the missions of the Green
peafowl Learning Centre (University of Phayao) is to promote children and youth to be the Green peafowl
protection youth network for study and learning in Green peafowl biology, peafowl food plants, and ecotourism.
The focus of our network are 1) to aware of changes in the status of Green peafowl, 2) to explore, to study,
and to participate in activities which promoting conservation of Green peafowls in and out the University’s area.
Green peafowl’s youth conservation network has operated for 3 years. There are 3 generations, 14 schools in
Phayao province with a cooperation by Demonstration School, University of Phayao (DeSUP). The goal of the
year 2021-2024, green peafowl youth camp and green peafowl biology meeting are to expand the youth network
to Chiang Rai, Phrae, Nan, Chiang Mai, Lamphun, Lampang, Mae Hong Son, Tak, Sukhothai, and link to
academic institutes in different regions across Thailand, Yunnan province of China, and the Salween - Mekong
River Basin. Green peafowls will be used as a goodwill ambassador in education, economics, tourism, and
innovations.

Keywords: Bird life, Endangered species, Green peafowl-people community, Green peafowl network,
Wild life

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วิจัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

BIO-O-25 25

Variation of Apis cerana indica populations (North, South and Hybrid) based on geometric
morphometric on forewing structure

Jakaphan Sunut1, Maneenuch Runsri1 and Tipwan Suppasat1,*
1Biology Program, School of Science, University of Phayao, Phayao, Thailand 56000
* Corresponding author, e-mail : [email protected]

Apis cerana indica is native to Thailand and throughout the country distributed. Morphological characters are
differences according to the geographical distribution of the Isthmus of Kra area, separated into two groups
including North and South populations. At present, there is more widespread of A. cerana beekeeping in 2018
colonies of A. cerana South populations were moved to the agricultural fields of Phayao Province for A. cerana
hybrid bee breeding project. The objective of this study was to examine variations of the A. cerana populations
from natural nests, North (n=22 colonies), South (n=6), and hybrids (n = 25) by using geometric morphometric
based on 19 landmarks on the vein junction of the forewing (n = 527). The results of this examination of the A.
cerana forewing found three different patterns: normal wing pattern (49.72%), irregular wing vein at the distal Rs
Marginal cell (46.11%) and 2rs-m (4.17%). The geometric results showed the highest variance on landmark 15
which is at the end of the marginal cell. The discrimination of relative warp results indicated the efficacy of
classification of A. cerana from North, South, and hybrids populations, were accurated to 77. 6% . Thus, it may
suggest that this method is effective in analyzing variation and monitoring changes in the forewing morphology of
A. cerana. And maybe applied as a morphological marker to examines morphological variations for A. cerana
breeding project in Thailand.

Keywords: Apis cerana indica, Geometric morphometric, Variation, Wing

การประชมุ วชิ าการระดับชาติ วทิ ยาศาสตรว์ จิ ัย คร้งั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-O-26 26

Geometric morphometric on forewing of Apis mellifera and Buckfast bee population in
Thailand

Kittiyaporn Nimprom1, Siraprapar Jansamak1 and Tipwan Suppasat1,*
1 Department of biology School of science University of Phayao 56000
* Corresponding author E-mail : [email protected]

Apis mellifera was imported to raise the agricultural occupation of Thailand. Currently, there is considerable
mixing distribution of honey bee populations in each bee farms. The objective of this study was to examine the
A. mellifera forewing variation by the geometric morphometric of 1,576 specimens from 161 colonies. Nineteen
landmarks were plotted as an indication of the vein junction on the forewings. The results showed that the A.
mellifera populations and the Buckfast bees could be correct identified as 94.2% and were able to distinguish
9 populations from different farms or different collection periods by using the geometric morphometric analysis.
Moreover, the variation in the breeding bee populations of the Panya bee farm collected in 2017 and 2020, the
Buckfast bee, and their hybrid populations could be correct identified as 80.0%. The study concluded that the
geometric morphometric analysis on the forewing could be effective in identifying the variations in Thai A.
mellifera, Buckfast bee and their hybrid bee populations. This information supports the application of the
geometric morphometric on the forewings to be used as a morphological marker for further investigation of the
origin of the A. mellifera subspecies breeding in Thailand.
Keywords : Geometric morphometric / Apis mellifera / Buckfast bee / Wing

การประชุมวิชาการระดับชาติ วิทยาศาสตร์วิจัย คร้ังท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

Oral Presentation

กลมุ่ ที่ 2
สาขาวชิ าเคมี เคมีประยุกต์ และเคมสี ่ิงแวดล้อม

การประชมุ วิชาการระดับชาติ วิทยาศาสตรว์ จิ ยั คร้ังที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

CHEM-O-01 27

Factors Effecting the Formation Polymer Droplets in a Novel Porous Hydrogel System

Thanyaporn Pinthong 1, Sukunya Ross 1, Sararat Mahasaranon 1, Gareth M Ross 1*
1 Biopolymer group, Department of Chemistry, Faculty of Science,
Naresuan University, Phitsanulok, 65000 Thailand
 Corresponding author, email: [email protected]

Hydrogels are hydrophilic cross-linked polymer networks that possess many excellent properties for use in
various applications e.g. wound dressing, tissue engineering, cosmetics and contact lenses. This research
focuses on a series of developed hydrogels which are fabricated with a porous structure via polymerization in
the presence of a blowing agent. These porous hydrogels were developed to adsorb large amounts of water.
These porous hydrogels were characterized traditionally first by bulk swelling and morphology. The morphology
was measured by scanning electron microscopy (SEM) to observe the pore size and structure, the micrographs
were analysed using ImageJ to obtain the pore size and the results showed an average 97.7 and 39.1 µm of
cross-section and surface pores, respectively. The SEM images of the hydrogel cross-section also presented
polymer droplets in the structure. The formation of these droplets significantly governs the mechanical
performance of samples, especially after swelling. Therefore, the factors that affect their formation were
thoroughly investigated by altering the gels composition. This was achieved by varying the concentrations and
type of surfactant, including the use of a dual surfactant system. The samples were tested to observe how
altering composition influenced polymer droplet size and formation and subsequently how this effected the key
properties, including bulk swelling and dynamic mechanical analysis. The results showed that surfactant type
and amount greatly altered the formation of polymer droplets and also the mechanical properties of the gels,
especially, after the incorporation of more water into the system. By careful selection of the system composition,
it is possible to produce porous hydrogels with outstanding swelling performance while still maintaining good
mechanical strength.

Keywords: Porous Hydrogels, Surfactants, Superabsorbent polymers

การประชุมวิชาการระดับชาติ วทิ ยาศาสตรว์ จิ ัย ครง้ั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-O-02 28

Fabrication of biodegradable ternary blend film based on poly (lactic acid),
polycaprolactone and cellulose acetate butyrate (PLA/PCL/CAB): miscibility study and
mechanical properties

Nantaprapa Tuancharoensri 1, Gareth Ross 1, Sararat Mahasaranon1 and Sukunya Ross 1, *
1 Biopolymer Research Group, Department of Chemistry and Biomaterials Center of Excellence, Faculty of Science, Naresuan
University, Phitsanulok, 65000 Thailand
* Corresponding author email: [email protected]

Biodegradable polymer based on poly ( lactic acid) ( PLA) are widely used in several application such as
packaging, textile, automotive-transport, agriculture and electronics. Naturally, PLA is too brittle and is therefore
usually not compatible to produce the packaging products. Therefore, in this work we strengthened with
polycaprolactone ( PCL) and cellulose acetate butyrate (CAB) in order to enhance the films performance.
Ternary blended films of PLA/PCL/CAB were successfully fabricated via blow film extrusion. Firstly, the
preliminary predictions of miscibility between pair polymers (PLA/PCL, PLA/CAB and PCL/CAB) were studied
using the Coleman and Painter approach. It is known that PLA/PCL forms immiscible blends and shows critical
solubility parameter differences (∆δ) of 1.8 (cal cm–3)1/2 while PLA/CAB can form good miscible blends (∆δ =
0.1 (cal cm–3)1/2) and PLC/CAB (∆δ = 1.7 (cal cm–3)1/2) may form miscible or immiscible blends depending on
molecular interactions. This demonstrates that CAB is able to act as a compatibilizer. After that a rapid scanning
method of solvent blending was used to identify miscible regions of both binary and ternary blended films. The
blending compositions of PLA/PCL/CAB were selected to be blown into the films based on the preliminary
guideline from the optical ternary phase diagrams. The mechanical properties (tensile strength, elongation and
modulus), crystallinity and miscibility of the blended film were investigated by universal tensile machine, XRD
and ATR-FTIR analyses, respectively. The ternary blend film at 70/15/15, 65/15/20 and 60/15/25 showed higher
percentages of elongation at break with increasing CAB content, due to greater H-bonding between PLA and
CAB. Moreover, the good miscibility depended on the molecular interactions between PLA/CAB and PCL/CAB.
In summary, these biodegradable ternary blend films demonstrate good performance in terms of the increase
percentage of elongation at break and miscibility, thus, have potential for use as flexible films in food packaging.

Keywords: Biodegradable films, Poly (lactic acid) (PLA), Polycaprolactone (PCL), Ternary blend film,
Miscibility

การประชมุ วิชาการระดับชาติ วิทยาศาสตรว์ ิจัย ครั้งที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-03 29

The properties of biodegradable packaging films based on polybutylene succinate (PBS)
incorporated with natural colorant from Hibiscus sabdariffa L. roselle calyx and
Caesalpinia sappan L. heartwood extraction.

Worraphol Nansu, Sukunya Ross, Gareth Ross, Nungruthai Suphrom and Sararat Mahasaranon*
1 Department of chemistry, Faculty of Science, Naresuan University, Phitsanulok, 65000
* Corresponding author email: [email protected]

This research is interested in developing biodegradable packaging applications based on bioplastic polymer
which is polybutylene succinate (PBS). Biodegradable packaging was incorporated with a natural colorant that
is Roselle (Hibiscus sabdariffa) and Sappan heartwood (Caesalpinia sappan Linn.). 0, 0.1, 0.2, and 0.3%wt of
natural colorant extraction was loaded into PBS pellets and formed by twin screw extrusion and blown film
extrusion. Biodegradable packaging film was characterized by colorant index, water vapor transmission rate
(WVTR), UV-vis light barrier properties, morphology, chemical properties, crystallinity properties and mechanical
properties. Results showed the color of biodegradable packaging film that presented pink to the purple tone of
roselle film and orange tone of sappan film. Moreover, WVTR of film incorporated with roselle and sappan
extract was slightly decreased when the concentration of natural colorant increased. UV-Vis barrier property
was found and the decreased light transmittance and mechanical properties of biodegradable packaging film
were improved in terms of tensile strength and %elongation. The polybutylene succinate (PBS) film incorporated
with natural colorant can be applied as biodegradable packaging material, nontoxicity, biodegradability and
environmentally friendly.
Keywords: Biodegradable packaging films, PBS film, Roselle calyx, Sappan heartwood

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วิจัย ครั้งที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-O-04 30

Hybrid scaffold of PLAV/SSV crosslinked-microgels incorporated with PLGA electrospun
nanofibrous for skin tissue regeneration

Arisa Kongprayoon1, Gareth Ross1,2, Sararat Mahasaranon1,2 and Sukunya Ross1,2*
1 Master Program in Industrial Chemistry, Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok,
65000
2 Biopolymer Group, Excellent Center of Biomaterials, Department of Chemistry, Faculty of Science, Naresuan University,
Phitsanulok, 65000
* Corresponding author email: [email protected]

This work is concerned with the fabrication of a hybrid scaffold from microgels incorporated with electrospun
nanofiber for skin tissue regeneration, which are both biocompatible and biodegradable materials. Poly(lactic
acid) (PLA) and silk sericin (SS) were first modified at their chain-ends by adding vinyl groups from ally glycidyl
ether (AGE), to be polymerizable poly(lactic acid) (PLAV) and polymerizable silk sericin (SSV). Microgels were
synthesized to PLAV/SSV microgels with and without crosslinker (N’N-methylenebis acryamide) by using free
radical polymerization. PLAV/SSV crosslinked-microgels showed spherical shape morphology, homogeneity,
diameter size in the range of 400-600 nm, which are able to entrap and release antibiotic drug (hydrophilic
drug) in 48 hours. These microgels were incorporated with PLGA nanofiber using a colloid-electrospinning
technique, to give the “hybrid scaffolds”. The morphology of hybrid scaffolds was smooth, being continuous
fibers and diameter size in the range of 1500-2000 nm. The in vitro cell viability of this scaffold is in the range
of 80-100%. In summary, this novel hybrid scaffold can be used for biomedical applications due to their non-
toxicity and biocompatibility that favour for normal human dermal fibroblasts.

Keywords: Hybrid scaffold, Microgels, Electrospun nanofibrous, Skin tissue regeneration

การประชมุ วชิ าการระดับชาติ วทิ ยาศาสตรว์ จิ ัย ครั้งท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-O-05 31

Fabrication and characterization of Prosthetic arms using 3D-printing technique: Fused
Deposition Modeling (FDM)

Amonrut Waisarikit1, Sukunya Ross1, Gareth M. Ross1, Nuntawat Udee2 and Sararat Mahasaranon1,*
1 Biopolymer Group, Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand
2 Department of Radiological Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand
* Corresponding author email: [email protected]

This research was interested in using a computed tomography (CT) scanner and FDM 3D printing for producing
customized prosthetic arms. The main objective of these studies were the orientation of printing (horizontal or
XYZ and vertical or ZXY axis) and the effects on the physical (morphology) and mechanical properties (tensile,
flexural and impact strength) of the customized prosthetic arms. CT-scanner was used to create high accuracy
medical information to produce customized prostheses by 3D-printing. The disadvantages of traditional devices
such as high cost and poor fitting to individual patients are the major reasons to change to use 3D-printing.
FDM 3D-printing materials were used 4 different polymer filaments that were poly(lactic acid) or PLA,
acrylonitrile butadiene styrene or ABS, polyethylene terephthalate glycol or PETG and poly(lactic acid)/carbon
fiber or CFPLA. The morphology of PLA, ABS and PETG specimens shows a smooth surface and less void in
the fracture surface when compared with CFPLA by SEM image. The result of PLA specimens showed higher
mechanical strength than CFPLA, PETG and ABS, respectively. XYZ axis printing direction shows high tensile
strength, flexural strength and impact strength when compared with ZXY axis orientation. The lower strength
of ZXY axis was due to distortion between the layers of polymer and voids in the polymer matrix. The results
of PLA and CFPLA in XYZ axis showed higher tensile strength, of 46.40 and 39.88 MPa, respectively.
Therefore, these were the suitable machine parameters for fabricating the standard specimens specifically
personal prosthetic arms from high-quality medical images (Anthropomorphic Phantoms) using a CT scanner.
Moreover, the prosthetic arms manufactured from 3D printing, which is highly accurate in producing prototypes
are lightweight and fit the individual needs of the patients.

Keywords: 3D-printing, Fused Deposition Modeling (FDM), Computed tomography scanner, Anthropomorphic
Phantoms, Prosthetic arms

การประชมุ วิชาการระดับชาติ วิทยาศาสตรว์ จิ ัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-06 32

Fabrication and characterization of biodegradable and biocompatible film based on
poly(vinyl alcohol) and silk sericin incorporated with Chromolaena Odorata (L.) leaves
extract for hemostasis activities

Sudarat Promkrainit1, Gareth Ross2, Sararat Mahasaranon2, Sukunya Ross2*
1Master Program in Industrial Chemistry, Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000,
Thailand
2Biopolymer Group, Excellent Center of Biomaterials, Department of Chemistry, Faculty of Science, Naresuan University,
Phitsanulok 65000, Thailand
* Corresponding author email: [email protected]

This research work was concerned with the fabrication and characterization of biodegradable and biocompatible
films based on poly(vinyl alcohol) and sericin, incorporated with Chromolaena Odorata (L.) leaf extract for
hemostasis activities. Samples were fabricated with different Chromolaena Odorata (L.) leaf extract
concentrations. To prepare the samples, 10 g Chromolaena Odorata (L.) leaves were extracted using 100 ml
of 95% v/v ethanol. A 10% w/v of poly(vinyl alcohol) (PVA) solution and silk sericin from silk cocoons were also
prepared. To fabricate PVA/SS films, poly(vinyl alcohol) solution was mixed with silk sericin and different
concentrations of Chromolaena Odorata (L.) leaf extract, these were at 0, 2, 5 and 10 %w/v, respectively. The
mixing was carried out at 100 °C in a close system followed by the addition of N,N’-Dimethylolurea (DMU) as
a crosslinker. Finally, the samples were fabricated and characterized. For a film forming technique, 100 μl of
sample solution was dropped by micropipette and then left in an oven at 60 °C for 15 hours. The PVA/SS films
were characterized for their functional groups by Fourier Transform spectroscopy (FT-IR). The functional groups
present are C-H stretching aromatic and C=C ring stretching that appears at 2998.87 and 1455.43 cm-1
respectively. These peaks can confirm the existence of Chromolaena Odorata (L.) leaf extract in the PVA/SS
films. Other characterization techniques included morphology (by Scanning Electron Microscope) and swelling
ratio. The sample, which have high content of Chromolaena Odorata (L.) leaf extract, show higher swelling
ratio than other samples. Films of PVA/SS/Chromolaena Odorata (L.) leaf extracts were homogeneous at all
compositions, excluding 10 %w/v sample. This work has the potential for using in medical applications such as
hemostasis activities and wound healing.

Keywords: chromolaena Odorata (L.) / hemostasis activities / poly(vinyl alcohol) (PVA) / silk sericin /
biodegradable and biocompatible films

การประชมุ วิชาการระดับชาติ วิทยาศาสตร์วิจัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-07 33

FABRICATION OF pH-RESPONSIVE SMART HYDROGELS FOR CONTROLLED RELEASE
SYSTEM

Maytinee Yooyod1, Sukunya Ross1 and Gareth Ross1*,
1 Biopolymer group, Department of chemistry, Faculty of science, Naresuan University, Phitsanulok, 65000, THAILAND
* Corresponding author email: [email protected]

This work is concerned with the fabrication of pH responsive hydrogels and their potential ability as delivery
carriers for cosmetically active ingredients. This was achieved by producing hydrogels that contain the
polyvinylamine (VAm). We first prepared a poly(N-vinyl formamide)-co-poly(N-hydroxyethyl acrylamide)
(poly(NVF-co-HEA)) hydrogel via photo-polymerization technique using a cross-linking agent. This gel was then
hydrolyzed to convert the vinylformamide groups in the polymer to vinylamine, the hydrolysis was carried out
under acidic conditions, at 80 °C using 0.01 M aqueous HCl. These pH sensitive hydrogels were characterized
by EWC, DSC and swelling behavior in both water and ethanol. The hydrogel swelling at different pH was
observed, non-hydrolyzed hydrogels (poly(NVF-co-HEA)) swelling was consistent across the entire pH range
but after hydrolysis the hydrogels show different behavior with reduced swelling ratios observed at acidic and
basic conditions (pH2 and pH10 and12). In our design of a controlled release system, Lactobionic acid was
incorporated as model ingredient. For in-vitro study, this methodology allows the detailed understanding of the
active delivery properties to be accessed. Samples were collected at various time intervals over a selected
time period. All the results show that the functionalised hydrogel were successfully prepared in the form of
poly(NVF-co-HEA-co-PVAm) hydrogels. The synthesized hydrogels in this study have the potential to be used
as a smart carrier for cosmetic ingredients triggered by an external pH change for cosmetic applications as this
gel exhibits pH dependent behavior.

Keywords: controlled release, cosmetic ingredients, pH responsive hydrogels, poly(vinylamine) (PVAm)

การประชุมวิชาการระดับชาติ วทิ ยาศาสตร์วิจัย ครั้งท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-08 34

Synthesis and characterization of silk sericin carbon dots

Areeya Taunchai1, Sasimontra Timjan2, Gareth M. Ross3, Sararat Mahasaranon3, Sukunya Ross3,*
1Program in Industrial Chemistry, Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand
2Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand
3Biopolymer Group, Excellent Center of Biomaterials, Department of Chemistry, Faculty of Science, Naresuan University,
Phitsanulok 65000, Thailand
* Corresponding author email: [email protected]

Carbon dots (CDs) are small carbon nanoparticles with a dimension (less than 10 nm in size) with some form
of surface passivation, with excellent properties, such as absorption in both the ultraviolet and visible regions,
tunable photoluminescence, high photostability and low toxicity thus high biocompatibility. In this work, we used
Bombyx mori silk, as a raw material to prepare photoluminescent carbon dots. The CDs were synthesized by
a hydrothermal method and the prepared CDs with silk sericin (SS) from bombyx Mori silk cocoons a material
made from natural product, which have a higher PL quantum yield than other raw materials such as grass, egg
and soy milk. Therefore, these CDs are expected to have a high PL quantum yield. This research characterized
the CDs based on SS by Fourier-transform infrared spectroscopy, UV-Vis absorption and Fluorescence
emission and drug loading capacity. The results showed that CDs prepared from SS at 220๐C for 6 hours, the
FTIR showed transmittance peak at 3061.29 cm-1 and 2931.97 cm-1, which are assigned to N-H stretching
vibration and C-H stretching. The UV-VIS absorption spectra of CDs showed peaks at 275 nm and 320 nm.
Fluorescence emission of CDs seemed to excitation wavelength increase at 320 nm and 360 nm. These
functional groups on the CDs surface affect the drug release behaviors and the aggregation of drug model
(rhodamine B) on the CDs surface resulting in the fluorescence quenching. In this study, the fluorescence CDs
were successfully synthesized from SS and the high fluorescence intensities on blue light, the samples prepared
from SS to provide surface passivation for drug delivery. The method of production CDs with fluorescent are
cheap and simple.

Keywords: Carbon dots (CDs), Silk sericin (SS), hydrothermal method, Drug release

การประชุมวชิ าการระดับชาติ วิทยาศาสตร์วิจัย ครัง้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-O-09 35

Anti-bacterial and Biodegradable Plastic Film for Food Preserving Packaging

Sasikanchana Khongraksa 1,*, Prapawarin Wanabodeenimit 2 and Voratha Assavasirijinda 3
1Piboonbumpen Demonstration School, Burapha University, Chon Buri, 20131
* Corresponding author email: [email protected]

Plastic waste is an extreme problem affecting our environment due to the disability of plastic to degradation in
a short period of time by natural processes. Moreover, some parts of plastic break into small pieces called
microplastic. According to this reason, the aim of research was the preparation of plastic film from biodegradable
polymer to use instead of non-biodegradable plastic. Poly(lactic acid) (PLA) and poly(butylene succinate) (PBS)
were used in this study. The biodegradable plastic film was produced by a solvent casting method using
dichloromethane and chloroform as solvent. After the drying process, the plastic film was obtained. The effects
of polymer concentration, polymer blending ratio, rating and temperature while blending, drying procedure, and
plasticizer were studied. The physical, chemical, and mechanical properties of obtained film were evaluated. In
addition, the enhancement of antibacterial activity of biodegradable plastic film was also studied by adding
sorbic acid as an anti-bacterial additive.

Keywords: Biodegradable cling film , food packaging , Film-casting , Antibacterial and Polylactic
acid

การประชมุ วิชาการระดับชาติ วทิ ยาศาสตรว์ ิจัย ครัง้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-10 36

Fabrication of Advanced Hydrogels Based on Functionalised Sulfonate Macromers

Jinjutha Daengmankhong1, Sukunya Ross1, Sararat Mahasaranon1, Gareth Ross1*
1 Biopolymer Group, Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand
* Corresponding author email: [email protected]

Macromers in this work are defined as pre-polymerized monomers that are decorated with polymerizable double
bonds along the polymer chain, which can enable rapid further polymerization or cross-linking. Macromers
possess several advantages such as they tend to display less toxicity than encountered with low molecular
weight monomers, even when possessing the same chemical structures. This work focused on a series of
novel sulfonate based macromers, synthesized through radical polymerization of 2-acrylamido-2-methylpropane
sulfonic acid (AMPS) as main monomer and 2-carboxyethyl acrylate (CEA). CEA contains a carboxylic acid
pendant functional group which can be modified to form the macromer. In this modification process, allyl glycidyl
ether (AGE) was used to leave pendant vinyl groups by a ring opening reaction. The fabricated water soluble
macromers were then ready to synthesize into other material forms such as hydrogels. The main focus of this
work was on initial acid monomer concentration (degree of polymerizable groups) and controlling the length of
the polymer chains via polymerization time. Moreover, different copolymer ratios were studied to help enhance
mechanical strength of the subsequent hydrogels by adding amounts of N-Hydroxyethyl acrylamide (HEA) and
3-sulfopropyl acrylate potassium salt (SPA). The synthesized macromers were preliminary characterized for
their physical properties by using solubility test and 1H NMR to prove the successful modification reaction. The
macromers were then formed into hydrogels via a cross-linking agent. The effect of macromer structure on the
hydrogel formed was examined by gelation time and gel strength of the hydrogel matrix. For example, the
gelation was altered from 30s to 10 mins by altering the number of polymerizable group in the macromer and
polymer chain length. These designed macromers provide an alternative method in fabricating hydrogels, which
can give rise to better in-situ formation of hydrogels for various biomedical applications in the future.

Keyword: Macromer, Hydrogel, Biomedical Application

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วจิ ัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-O-11 37

Synthesis and characterization of graphene oxide from agricultural waste

Aphitchaya Arunchaeng1, Worapitcha Yusuk1, Pornnipa Nunocha2 and Tawat Suriwong2,*
1Naresuan University Demonstration School, Phitsanulok, 65000
2School of Renewable Energy Technology, Naresuan University, Phitsanulok, 65000
* Corresponding author email: [email protected]

Graphene oxide (GO) is a single layer of oxygenated graphite holding functionalities. GO is a two-dimensional
honeycomb carbon with sp2 hybridization. It has been shown in various properties. GO can be synthesized
using chemical and mechanical methods. This project synthesized graphene oxide using Hummer’s method
which is about destroying forces between graphite oxide layers using the differences of temperature. The
products were in the form of thin dark films. GO can be characterized using XRD, Raman, FTIR, and SEM
techniques. XRD results shown peaks 2 at 12.50 and 42.50 cm-1 in all conditions which is corresponding to
graphene oxide. Raman results showed the impurity and graphite state by the ratios of ID and IG peaks and
the ratio of I2D/IG peaks which showed that the obtained GOs were multilayers. FTIR was used to characterize
the functional groups in GOs which were O-H bond at peak 3,024 cm-1, carboxyl group at peak 1,715 cm-1,
water molecule absorbed at peak 1,617 cm-1, C-OH groups at peak 1,344 cm-1, C-O-C stretching at peak 1,221
cm-1, and C-O groups at peak 1050 cm-1. Furthermore, SEM results showed the arrangement of GO layers. If
pure graphite can be used to produce graphene oxide, agricultural graphite should be able to produce graphene
oxide as well. This project produced GO from agricultural graphite and compared the characteristics to natural
graphite using the same methods. Moreover, if GO is reduced, it will become rGO or reduced graphene oxide
which enhances the properties to be more graphene.

Keyword: Graphene oxide, Graphite oxide, agricultural graphite, Hummer’s method

การประชมุ วิชาการระดับชาติ วิทยาศาสตร์วจิ ัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-12 38

Structural, morphological, and optical properties of a copper bismuth oxide photocatalyst

Punyanuch Thammaacheep1, Kantapat Chansaenpak2 and Duangdao Channei 1,*
1 Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok, 65000
2 National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park,
Pathum Thani, 12120
* Corresponding author email: [email protected]

Heterogeneous photocatalysis is an effective technology for the removal of organic dyes in textile wastewater.
Metal oxide semiconductors have been extensively applied as photocatalysts in the last few decades. Herein,
we report the synthesis of copper bismuth oxide (CuBi2O4) photocatalyst by a facile precipitation method. The
crystal structure and phase composition of CuBi2O4 were evaluated by X–ray diffraction (XRD). The main
diffraction peaks could be indexed to the tetragonal structure of pure CuBi2O4. Reflectance spectra of CuBi2O4
were analyzed by UV–vis spectroscopy equipped with integrating spheres (UV–vis DRS). The absorption edge
was observed in the visible region which was associated with a narrow band gap (~2.0 eV). The photocatalytic
properties of CuBi2O4 were tested through the degradation of methylene blue (MB) dye pollutant under UV and
visible light irradiation. Based on the findings, CuBi2O4 photocatalyst is expected to be beneficial for the
treatment of such dyes in textile wastewater. Thus, further study to improve the photocatalytic activity of CuBi2O4
will have been done in our future work.
Keywords: CuBi2O4, Copper bismuth oxide, Photocatalyst, Photocatalysis

การประชมุ วชิ าการระดับชาติ วิทยาศาสตรว์ จิ ัย ครงั้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-O-13 39

Synthesis of 3,4-dihydropyrimidin-2(1H)-ones catalyzed by palladium on alumina under
solvent-free conditions

Sorachai Khamsan 1,* and Phawat Pienngam 2
1 Department of Chemistry, School of Science, University of Phayao, Phayao, 56000
2 Demonstration School University of Phayao, University of Phayao, Phayao, 56000
* Corresponding author email: [email protected]

Synthesis of 3,4-dihydropyrimidinone via one-pot three-component condensation reaction under solvent-free
conditions was developed. The optimum reaction conditions were 1.0 mmol of aromatic aldehyde compounds,
1.0 mmol of ethyl acetoacetate, 1.2 mmol of urea and 1% w/w of palladium on alumina at 80 ºC for 2 hours.
Compared to the classical Biginelli reaction, this developed synthetic protocol offers several advantages
including a simple, environmentally friendly method, and relatively short reaction times giving high yields.

Keywords: Dihydropyrimidinones, Palladium on alumina, Biginelli reaction, Green chemistry

การประชมุ วิชาการระดับชาติ วทิ ยาศาสตร์วิจัย ครง้ั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-14 40

The Development of Composite Adsorbent with NiAl- Layered Double Oxide and CaFe- Layered
Double Hydroxide to Remove Hexavalent Chromium from Wastewater Solution

Mesa Na Nakorn1 Anamika Kaewnok1 Panita Sumanatrakul2 and Sonchai Intachai2,*
1 Paphayompittayakom school, SCiUS – Thaksin University, Phatthalung, 93210
2 Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung, 93210
 Corresponding author, email: [email protected]

Nowadays there is an increasing of water pollution due to the widespread expansion of large factories,
communities and so on. It is well known that heavy metals such as Hg, Cd, As, Pb, Cr, etc. are severe
poison which mostly effect on carcinogenic, hepatic, respiratory, renal and so on. Hexavalent chromium
(Cr6+), a heavy metal species is commonly formed in complex or oxide anions especially dichromate (Cr2O72−)
where it is extensively utilized as catalyst, reactant and oxidant. The contamination of water with dichromate is
one of the major cause of water pollution and danger, so the elimination of hexavalent chromium from
wastewater must take actions urgently. This work is to study of removing hexavalent chromium in aqueous
solution by using composite adsorbent with NiAl-layered double oxide and CaFe-layered double hydroxide. These
materials possess large surface area and positively charge surface, and magnetic substance due to CaFe-layered
double hydroxide. NiAl-layered double oxide was prepared by the calcination of NiAl-layered double hydroxide at
400 C for 2 hours. CaFe-layered double hydroxide was prepared by hydrothermal method at 120 C for 48
hours. The solid-solid reaction was used to prepare magnetic composite by grinding NiAl-layered double oxide and
CaFe-layered double hydroxide with weight ratio =3:1 under acetone spraying for 30 minutes. All samples were
characterized by XRD, FTIR, SEM, VSM, BET and UV-VIS. The rest concentration of dichromate in water was
determined by UV-VIS. The removal percentage of hexavalent chromium reached 100, 98 and 87 of NiAl-layered
double oxide, magnetic composite and CaFe-layered double hydroxide, respectively. The result showed that NiAl-
layered double oxide composited with CaFe-layered double hydroxide was the best adsorbent for removing
dichromate form wastewater. In addition, the composite solid was easily separated from the solution with an
external magnet.

Keywords: Chromium, NiAl-Layered Double Hydroxide, NiAl-Layered Double Oxide, CaFe-Layered Double
Hydroxide, Adsorption, Composite Adsorbent

การประชมุ วชิ าการระดับชาติ วิทยาศาสตรว์ จิ ัย คร้งั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-15 41

Solid-State Mixing of NiAl-Layered Double Oxide, Activated Carbon and CaFe-Layered
Double Hydroxide for Removal of Both Congo Red and Methylene Blue in Water

Panpruek Pruekthikanee1 Jakkapat Sermkaew1 and Sonchai Intachai2,*
1 Paphayompittayakom school, SCiUS – Thaksin university, Phatthalung, 93210
2 Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung, 93210
* Corresponding author email: [email protected]

The contamination of toxic chemicals in natural water has still occurred continually that can be observed
apparently from colored and fetid water, as well the death of aquatic animals and plants. Synthetic dyes are
introduced in dyeing processes of many activities such as texture, car, furniture, etc., and the rest is left into
water that counted as a main cause of wastewater. Due to dyes categorized as severe dangerous chemicals
in spite of very low amount on health and environmental issues, the dyes contaminated in water resources
must be removed urgently. Based structural charge, anionic and cationic dye ions are mostly applied resulting
contamination in water resources. NiAl-layered double oxide (NiAl-LDO) and magnetic CaFe-layered double
hydroxide (CaFe-LDH) containing positively charged surface are extensively used as the adsorbent for
eliminating anionic dye such as Congo red, methyl orange and orange II. Meanwhile, cationic dye ions like
methylene blue and rhodamine 6G are eliminated by activated carbon adsorbent (AC). Because NiAl-LDO,
CaFe-LDH and AC possess large area surface and high stabilities on light and chemical exposure for long time
periods together with many time’s reusability. In this work, inorganic materials including of NiAl-LDO, AC
prepared from palm shell, and CaFe-LDH were composited by solid-solid reaction as the magnetic adsorbent
(NiAl-LDO/AC@CaFe-LDH) for removing both Congo red and methylene blue in aqueous solution. All samples
were characterized by XRD, FTIR, SEM, VSM, BET and UV-VIS. The dye concentration in each time interval
of the adsorption reaction was determined by UV-VIS. By using 60 mg of NiAl-LDO, AC, NiAl-LDO/AC@CaFe-
LDH and CaFe-LDH respectively, the removal percentage of methylene blue (60 mL 60 ppm) was 16, 100, 96
and 11, meanwhile that of Congo red was 98, 21, 94 and 84. The result showed that NiAl-LDO was selective
to bind Congo red anions, as well as AC was selective to bind methylene blue cations. NiAl-LDO/AC@CaFe-
LDH exhibited the good adsorbent for removing both anionic and cationic dye ions, besides its solid powder
was easily separated by magnetizing with the external magnet.

Keywords: Activated Carbon, CaFe-Layered Double Hydroxide, Composite, Congo red, Methylene Blue, NiAl-
Layered Double Oxide,

การประชุมวิชาการระดับชาติ วทิ ยาศาสตร์วจิ ัย ครงั้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-16 42

Cold pressed Bitter gourd oil for use in soap

Lalita rachawong and *Panida Saenprakob

1 Department of Cosmetic science, Faculty of Science and Technology, Phranakhon Rajabhat University

Corresponding author: [email protected]
Bitter gourd oil was extracted cold- pressed using a compression machine. Without heat This is a technique
suitable for natural substances that are sensitive to temperature. The extracted oil has a percentage yield of
1 0 m. It is a dark green liquid. Has a clear scent of bitter gourd. Then the extracted oil The chemical and
physical properties were analyzed for vitamin E using HPLC using column C18, using acetonitrile and maunthol.
Is the moving phase To inject the sample against the standard substance. 2 0 minutes after injection, it was
found that bitter melon oil contains vitamin E as an element. Since both samples appeared peak chromogram
at 1 2 minutes when bitter gourd oil was developed as an ingredient in bar soap. It was found that the soap
after five days incubation. With suitable hardness It has the pH of 9 and the solubility. And foaming was 243.34-
244.05 seconds and 0.01-0.03 centimeters, respectively. 6.43% bitter gourd oil by weight was a suitable ratio
for use as an ingredient in bar soaps and products that had a pale yellow color of the oil. And has the ability
to thoroughly wash off makeup
Keywords: Bitter gourd, Saop, Cosmetic, Vitamin E

การประชมุ วชิ าการระดับชาติ วิทยาศาสตรว์ ิจัย ครง้ั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-17 43

Petri dish plastic disinfectant from plant phenolic compound extract for substitute
chemical usage.

Tuwanon Kimchiang * and Panuphong Ratchasee
1 SciUS - Thaksin university, 222 Village No.2, Ban Phrao Sub-district, Pa Phayom District, Phatthalung, 93210
* Corresponding author email: [email protected]

A petri dish is an essential tool for microbiological experiments. Plastic Petri dishes are common and convenient
for preparing culture medium since they don't need to be sterilized before using them. However, it cannot be
reused and becomes a waste. The purpose of this research is to find effective plant extracts for cleaning used
plastic culture plates so that they could be reused for further culture media preparation. Five plants including
Piper betle (betel), Senna siamea (cassia), Ocimum × citriodorum (lemon basil), Ocimum tenuiflorum (basil),
and Centella asiatica (Gotu Kola) were extracted with ethanol as solvent. The analysis of total Phenolic
compounds and screening for antibacterial activities were studied. The results of the experiment showed that
the number of colonies after cleaning with the extract with the highest concentration (4000 ppm) gotu kola was
0.67, basil was 3.00, cassia was 3.67, basil was 8.67 and betel was 17.67. As a result, the best substance is
one that can disinfect the plastic petri dish. At a 4000ppm concentration, it's the same as a synthetic chemical.
Keywords: Petri dish plastic, Phenolic compound, plant extract

การประชมุ วิชาการระดับชาติ วทิ ยาศาสตร์วจิ ัย ครง้ั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-18 44

Theoretical Study of Cationic Metallocene Polymerizations of 2-Oxazoline

Natechanok Thinkumrob1, Wijitra Meelua1,2, Jitrayut Jitonnom1,*
1 Division of Chemistry, School of Science, University of Phayao, Phayao, 56000
2 Demonstration School University of Phayao, Phayao, 56000
* Corresponding author email: [email protected]

Polyoxazolines are gaining high interest in biomedical research as they can be made with high quality, of
different architectures, and with different functional groups. In this study, we report the density functional theory
studies on the cationic ring-opening polymerization (CROP) reaction of 2-methyl-oxazoline (MeOX) and 2-
phenyl-oxazoline (PhOX) by a zirconocene/borate catalyst, [Cp2ZrMe]+[B(C6F5)4-]. We used M06-2X/SVP for
geometry optimization and electronic property analysis. Detailed mechanism on the process is investigated and
useful insights are reavealed for catalyst activation, chain initiation and chain propagation. The influence of the
monomer sequence, solvent and cocatalyst on the process, are analyzed and they are key factors for effient
polymerizaton. This study provides a rationale for previous experimental observation, which could also be
helpful for future experimental design.
Keywords: Polyoxazoline, cationic polymerization, zirconocene, boron activator, DFT

การประชมุ วชิ าการระดับชาติ วทิ ยาศาสตรว์ ิจัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-19 45

Modeling enzymatic reaction mechanism and substrate selectivity of α-galactosidase with
QM/MM method

Tanchanok Wanjai1, 2, Jitrayut Jitonnom2, Wijitra Meelua1, 2*
1 Demonstration School University of Phayao, Phayao 56000, Thailand
2 School of Science, University of Phayao, Phayao 56000, Thailand
* Corresponding author email: [email protected]

The GH27 enzyme α-galactosidase (αGAL) plays an essential and unique role in the breakdown of α-
galactosides in the lysosome. Defects in human α-GAL lead to the development of Fabry disease. Many food
and feed industries nowadays utilize the enzyme to increase their production. Thus, the knowledge of catalytic
mechanism and the substrate specificity at atomic level would benefit its use, which are currently poorly
understood. Here, we applied quantum mechanical/molecular mechanical (QM/MM) molecular dynamics (MD)
simulations [at SCC-DFTB/CHARMM27] to study the enzyme structure and function in solution. The initial
structure of this study was taken from the X-ray structures of Saccharomyces cerevisiae GH27 α-galactosidase.
Three natural substrates, namely, melibiose, raffinose and galactomannan, were modelled in the active site of
the enzyme and their interaction and dynamic behaviours are investigated. Insights into enzyme-substrate
interactions during catalysis are well described at atomistic detail with computed potential and free energy
profiles support. The retaining mechanism is found for the enzyme with two carboxylate residues Asp149 and
Asp209 playing a key role during catalysis. The relative activation energies show a good trend with the
experimental kinetic data reported in the literature as melibiose < raffinose < galactomannose.

Keywords: QM/MM, α-galactosidase, enzyme mechanism, substrate specificity

การประชุมวชิ าการระดับชาติ วิทยาศาสตร์วิจัย ครั้งที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-O-20 46

Applying green analytical chemistry and smartphone technology for the development of
facile, cost-saving and effective miniaturized titration of sodium chloride injections

Theerasak Rojanarata *, Kanong Ruttanakorn, Noppharat Phadungcharoen, Jirapong Wongtha, Natchanon Lappongsombut,
Pathdara Kangtrakun and Tanabodi Charoenkitchatorn
1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Sanam Chandra Palace Campus,
Nakhon Pathom 73000, Thailand
* Corresponding author email: [email protected]

A facile, green and cost-saving miniaturized titration based on Volhard’s method was developed for the
determination of sodium chloride in injections. In the titration which was carried out in microcentrifuge tubes, a
titrant in the increasing volumes was added using a micropipette to a set of aliquots of sample containing ferric
ammonium sulfate as an indicator, covering both sides of the equivalence point. After being transferred to a
microplate and placed above an illuminating screen of a tablet, the solutions were photographed in one shot,
in a dark box using a smartphone camera. The red (R), Green (G) and Blue (B) pixels were read out from the
colors of the solutions, and a titration curve of 1/log G versus the volume of titrant was plotted and used for
determining the equivalence point which was located at the intersection of the two lines in the region before
and after the equivalence point. The assay was accurate (% recovery of 99.80) and precise (% R.S.D. for intra-
day and inter-day precision of 0.046 and 0.053, respectively), significantly reduced the use of reagents as well
as generation of waste, and required no expensive equipment. In addition, it was unaffected by the use of
different types of microplates, smartphones, and RGB analysis tools. To aid data processing and produce
reports in an instant, a mobile application was also developed for use with the assay, thereby providing a fast
and convenient means of analysis on a single smartphone device. Accordingly, the method was suitable for
routine quality control in the pharmaceutical industry and could be implemented as a small-scale experiment
for teaching chemistry in the digital era.

Keywords: Smartphone, Titration, RGB, Green analytical chemistry, Sodium chloride injections

การประชุมวิชาการระดับชาติ วทิ ยาศาสตรว์ ิจัย ครั้งที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-O-21 47

Development of porous chitosan, silk fibroin and poly (vinyl alcohol) scaffolds for dental
bone regeneration: Effect of crosslinker

Yeewa Sukkerd1, Gareth Ross1, Sararat Mahasaranon1, Winita Punyodom2,3, Anuphan Sittichokechaiwut4 and Sukunya Ross1*
1 Biopolymer Group, Department of Chemistry, Biomaterial Excellent Center, Faculty of Science, Naresuan University,
Phitsanulok 65000, Thailand
2 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
3 Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand
4 Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok 65000, Thailand
* Corresponding author email: [email protected]

This work is concerned with the development of scaffolds based on chitosan (CS), silk fibroin (SF) and poly
(vinyl alcohol) (PVA) for use in dental bone regeneration. The scaffolds were designed with interconnected
porous structures and developed by using natural and degradable materials such as; silk fibroin (SF) (helping
to stimulate cell adhesion and proliferation) and chitosan ((CS) enhancing bone regeneration), cooperated with
poly (vinyl alcohol)(PVA)(scaffold support) and a crosslinker (dimethyl urea, DMU) to strengthen the physical
properties of the scaffolds. The porous structure of the scaffolds was prepared by lyophilisation after mixing a
solution of the desired compositions at 60 ๐C. The scaffolds were prepared from CS/DMU at concentration 1.4
%w/v of CS and PVA/CS/DMU at concentration (5:1.4:3.75 %w/v). This research work, focused on the effects
of DMU with different concentrations of 5, 10, 15 and 20 %w/w studied. The porous scaffold of the CS with
DMU scaffolds at 20 %w/w showed high amounts of interconnecting porous structure with pore size diameter
between 50-100 µm. When, adding PVA and SF into the scaffolds, the mixture solution at 20 %w/w DMU still
contained interconnected porous structures with pore size diameters between 10-100 µm. The effects of
concentration of DMU onto the scaffolds gave regularity to the porous structure, while adding SF enhanced the
size of the porous structure with interconnecting structures as well as promoting improved flexibility in the
scaffolds. In summary, this PVA/CS/SF/DMU scaffold can be used for dental bone regeneration and promotes
osteoblasts formation. The in vitro cell viability of this scaffold is in the range of 70-94%.

Keywords: Dental bone regeneration, Scaffolds, Silk fibroin, Chitosan, Poly (vinyl alcohol).

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
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CHEM-O-22 48

QM/MM modeling of substrate specificity in α-galactosidase

Teeratchanid Kamkeaw1, Thamonwan Boonchom1, Wijitra Meelua1, Jitrayut Jitonnom1,2*
1 Demonstration School University of Phayao, Phayao 56000, Thailand
2 School of Science, University of Phayao, Phayao 56000, Thailand
* Corresponding author email: [email protected]

The enzyme α-galactosidase is a member of the family GH27 and it is responsible for accelerating the
degradation of α-glycosidic bonds in the lysosome. This enzyme is the target enzyme for the treatment of
Fabry disease. Generally, the detail catalytic mechanism and the substrate not well-understood. Consequently,
we used quantum mechanical/molecular mechanical (QM/MM MD) method, a technique that combines both
quantum mechanics world and the classical mechanics world in order to allow studying on a complex
biomolecular system such as enzyme. The X-ray structures of Saccharomyces cerevisiae GH27 α-
galactosidase in complexed with its natural substrate were used as a starting point for the present calculations.
The results show that the calculated energy profiles for 15 simulation snapshot for glycosylation steps with
average barriers of 17.73 ± 3.06 and 18.27 ± 5.15 kcal/mol for melibiose and raffinose, respectively. Thus, the
barrier for raffinose hydrolysis is larger than the case for melibiose substrate, in agreement with the
experimental observation.
Keywords: α-galactosidase, reaction mechanism, substrate specificity, computational chemistry

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CHEM-O-23 49

Understanding of regioselectivity in the alpha-galactosidase with QM/MM

Krongsiri Pongfai1, Phiriyaporn Jen-aksorn1, Jitrayut Jitonnom2 and Wijitra Meelua1,2*
1 Demonstration School University of Phayao, Phayao 56000, Thailand
2 School of Science, University of Phayao, Phayao 56000, Thailand
*Corresponding author email: [email protected]

The enzyme α-galactosidase is classified into family 27 of glycosyl hydrolases, which is responsible for
breakdown of α-galactosides in the lysosome. In humans, mutations of the α-galactosidase gene cause
incomplete degradation of glycolipid and glycoproteins, resulting in Fabry disease. The enzymes α-
galactosidase catalyze the hydrolysis of terminal α-1,6-galactosyl units from galacto-oilgosaccharides. Here,
we employed quantum mechanical/molecular mechanical (QM/MM) method to study the substrate binding
structures and the reaction mechanism of the enzyme. Therefore, the aims of this study about reaction of linear
galactomannose (Galman) and branch galactomannose (Galman3) and study the specificity of yeast alpha-
galactosidase to linear galactomannose than branch galactomannose. The results found that the calculation of
energy profiles for 15 snapshots for glycosylation steps. The average barriers are 17.87 ± 3.80 kcal/mol for
Galman and 22.20 ± 2.11 kcal/mol for Galman3 [at SCC-DFTB/CHARMM27 QM/MM level]. The barrier for
Galman3 hydrolysis is larger than the case for Galman substrate. This result is in agreement with the
experimental observation where this ScACal enzyme is specifically hydrolyzed toward Galman. The stationary
points are obtained and elucidated.
Keywords: α-galactosidase, galactomannose, Fabry disease, glycosylation and QM/MM

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Oral Presentation

กลุ่มท่ี 3
สาขาวชิ าคณติ ศาสตร์ คณิตศาสตร์ประยกุ ต์

สาขาวชิ าสถิติ

การประชมุ วชิ าการระดับชาติ วทิ ยาศาสตรว์ ิจยั ครัง้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
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MATH-O-01 50

Some New Iterative Techniques for the Problems Involving Image Restoration

Chonjaroen Chairatsiripong1 and Tanakit Thianwan1*

In this research, we established and studied a new faster 3 step algorithm for solving fixed point problems of
a family of nonexpansive mapping. We proved a weak convergence of these algorithms and the convergence
behavior of the proposed method is proven and discussed. Using our main result, we obtain a new faster 3
step algorithm to solve image restoration problem, called the new faster inertial forward backward algorithm for
involving the sum of two proper lower semi-continuous and convex functions.
We give some applications to the obstacle problem and to minimization problems. To demonstrate the
applicability of our method, we solve image restoration problems. We present numerical computations of image
restoration problems comparing these algorithms to some other classical methods and show PSNR. Finally,
we compare different inertial parameters for study efficiency of our method for image restoration problem.
Keywords: Hilbert space, proximal method, fixed point theory, forward-backward algorithm, image

restoration problem

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MATH-O-02 51

On the stability of some pexiderized fuzzy number-valued functional equation

Jiraporn Kunrattanaworawong1 and Wutiphol Sintunavarat 2,*
1 Department of Mathematics and Statistics, Faculty of Science and Technology, Thammasat University Rangsit Center,
Pathumthani 12120, Thailand
2 Department of Mathematics and Statistics, Faculty of Science and Technology, Thammasat University Rangsit Center,
Pathumthani 12120, Thailand
* Corresponding author email: [email protected]

The main goal of this paper is to investigate the Ulam stability of the following pexider fuzzy number-valued
functional equation via the metric related to the Hausdorff metric defined on the class of special alpha-cuts of
fuzzy numbers:

rf  x + y  + sg  x − y  =  r + s  h ( x) +  r − s  h ( y) ,
 s   r   s r   s r 

where f , g, h are unknown fuzzy number-valued functions on a Banach space, r and s are given non-zero

real numbers. Our main result covers many cases of stability results of the fuzzy number-valued functional

equations.

Keywords: Functional equation, Ulam stability, Fuzzy number-valued mapping, Hausdorff metric

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MATH-O-03 52

Iterative methods for solving split feasibility problem and application

Chattarin Jomkham 1, Tanatcha Chaichana1 and Nattawut Pholasa1,2,*
1 Demonstration School University of Phayao, University of Phayao, Phayao, 56000
2 School of science, University of Phayao, Phayao, 56000
* Corresponding author email: [email protected]

In this project, we are studying iterative methods for solving the split feasibility problem (SFP), which has many
applications in signal processing. Then we introduce iterative methods is designed for solving SFP depends on
self-adaptive and line-search techniques. Finally, we give an example and numerical result for supporting our
main result and its applications to the signal processing problems.
Keywords: Iterative methods, signal processing, split feasibility problem

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

MATH-O-04 53

Coronavirus Disease 2019 (COVID-19) Dynamics: A mathematical modelling approach

Songheng Orn1,* and Wirawan Chinviriyasit2
1,2 Department of Mathematics, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok, 10140
* Corresponding author email: [email protected]

The aims of this paper are to formulate the model of Coronavirus Disease 2019 (COVID-19) based on the
epidemiology of COVID-19 and to analyse the local stability of the model. The threshold value of the model,
which is called the reproduction number and denoted by R0 , is derived. It is found that the model has a unique
disease-free equilibrium when R0 1 and it is locally asymptotically stable when R0 1. When R0 1, unique
endemic equilibrium exists and is locally asymptotically stable based on the use of the Centre manifold theory.
Keywords: Coronavirus, Equilibrium; Stability, Reproductive number

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MATH-O-05 54

Graceful labeling in the context of duplication of star graph in

Panida buapan1 , Siwaporn Saewan2
1 Siwaporn Saewan . Asst. Prof. Dr., Department of Mathematics and Statistics, Faculty of Science, Thaksin University,
Phatthalung, 93210, Thailand
* Corresponding author email: [email protected]

Let a simple graph be connected and undirected graph with vertices and edges. Let be a function
from the set of vertices to the set of integer {0,1,2,3, … , } and let ∗ be a function from the set of
edge to the set of positive integer {1,2,3, … , } defined as

∗( ) = | ( ) − ( )|
for all , ∈ ( ) and ∈ ( ). A function is called graceful labeling of a graph if is
injective and ∗ is bijective. The graph which admits graceful labeling is called a graceful graph. In
this paper, we find graceful labeling for some new graphs obtained from the duplication of edge of star
and then we prove that the new graphs are graceful.
Keyword: graceful graph ;graceful labeling ;star graph

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วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

MATH-O-06 55

The number of k - symmetric numerical semigroup 0 a, b c,)

Praifa Kosasirisin * and Ekkachai Laysirikul
Department of Mathematics, Faculty of Science, Naresuan University, Phitsanulok, 65000
* Corresponding author email: [email protected]

Let a, b, c  be such that 2  a  b  c −1. Denote the set of integer x such that a  x  b by a,b
and the set of integer y such that y  c by c,) . Then 0 a,b c,) is a numerical semigroup

if and only if c  2a . Let U =  S : S = 0 a,b c,) is a numerical semigroup  . In this research,

we count the number of all k - symmetric numerical semigroups in U which having the same Frobenius
number where k  3 . Moreover, we determine the number of all 3 - symmetric numerical semigroups in U
which having the same genus number.
Keywords: Frobenius number, Genus number, k - symmetric numerical semigroup.

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