Abstracts

BIO-P-05 100

Effect of sucrose concentrations and air-drying on survival and regrowth of Cymbidium
mastersii Griff. ex Lindl. artificial seeds

Julaluk Linjikao1, Kwanchanok Meemas1 and Anupan Kongbangkerd1,*
1Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000
Corresponding author email: [email protected]

In vitro preservation of Cymbidium mastersii Griff. ex Lindl. protocorms by artificial seeds is an alternative
method for short to long-term storage of orchid germplasm. In this study, the effect of sucrose concentrations
and air- drying ( 0- 8 h) on the water contents, survival rate and percentages of morphological changes of
encapsulated C. mastersii protocorms were investigated. The optimal sucrose concentrations and dehydration
times for encapsulated protocorms were obtained when encapsulated protocorms were precultured with 0.2 M
sucrose for 3 days and dehydrated for 4 hours which gave the percentage of water content in beads at 27.6%,
survival rate at 7 7 . 8 % and morphological changes percentages at 69. 9% . After 10 weeks regrowth, the
dehydrated-encapsulated protocorms produced a maximum of 4.2 protocorms per explant. Consequently, the
above conditions seemed to be optimal for long-time cryopreservation of C. mastersii.
Keywords: Cymbidium mastersii Griff. ex Lindl. / Artificial seeds / Sucrose concentrations / Air-drying

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
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BIO-P-06 101

Effect of cytokinins on growth and development of shoot tips of Pleione maculata (Lindl.)
Lindl. & Paxton (Orchidaceae) in vitro culture

Thanakorn Wongsa1,*, and Nuengruethai Jucksri1
1Program in Biology, Faculty of Science and Technology, Kamphaeng Phet Rajabhat University, Kamphaeng Phet 62000
*Corresponding author e-mail: [email protected]

Pleione maculata (Lindl.) Lindl. & Paxton is rare orchid which a few micropropagation studies have been
reported. The effect of cytokinin on the multiplication was studied. In vitro shoot tips of Pleione maculata (Lindl.)
Lindl. & Paxton were cultured on ½ MS (Murashige and Skoog, 1962) supplemented with 30 g/l sucrose and
cytokinin; N6-Benzyladenine (BA), Kinetin (Kn) and Thidiazuron (TDZ) in various concentrations at 0, 0.1, 0.5,
1.0 and 2.0 mg/l for 8 weeks. The results found that the highest number of shoots could obtain on ½ MS
medium supplemented with 0.5 mg/l BA (3.3 shoots/explants). Furthermore, shoot tips were induced 1.7
Protocorm Like Bodies (PLBs) when cultured on ½ MS medium added with 5 mg/l Kn or 0.5 mg/l TDZ.
Keywords: Pleione maculata, cytokinins, growth and development

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วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-07 102

Changes in Physical and Chemical During storage of Huai Mun Pineapples

Warunee Chomkitichai1,* Kadtaleeya Nounthong2 Pornteera Sompanwang2 and Pairin Anuun2
1 Department of Biology, Faculty of Science and Technology, Uttaradit Rajabhat University, Uttaradit, 53000
2 Department of Biology, Faculty of Education, Uttaradit Rajabhat University, Uttaradit, 53000
* Corresponding author email: [email protected]

Huai Mun pineapple is one of the most important fruit in Uttaradit Province that is a favorite fruit of consumers.
The objective of this research was to study the physical and chemical changes during storage of Huai Mun
pineapple ( Ananas comosus L. Merr. ) . The harvest- stage pineapple were kept and stored at the room
temperature (30  1 C) for 7 days. The pineapple were randomized and analyzed the physical and chemical
changes in three parts: proximal part, middle part and distal part. The physical changes of peel color were
analyzed by visual evaluation and color meter. The chemical changes, including pH, titratable acidity (TA), total
soluble solid (TSS) and vitamin C content were analyzed from pineapple juice. The results showed that, during
storage, the peel color of Huai Mun pineapple had increases more yellow, beginning from the proximal part to
the middle part until the distal part, respectively. Nonetheless, there were small changes in pH, the titratable
acidity, the total soluble solid and the quantity of vitamin C content in each part of the fruit during storage. The
finding also showed that the total soluble solids at the proximal part of the fruits were significantly higher than
the others parts. This data can be applied to buying and processing of pineapple fruits in the further.
Keywords: Huai Mun, pineapple, chemical change, physical change

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BIO-P-08 103

Development of Serum from Bromelain Extract from Huay Mon Pinepple

Suttida Wittanalai1* PimSiri Kongto2 Janjira Kaewklao2 Pimpan Srijan2 and Attasan Nakornsri2
1 Biology Program, Faculty of Science and Technology, Uttaradit Rajabhat University, Uttaradit 53000, Thailand
2 Biology Program, Faculty of Education, Uttaradit Rajabhat University, Uttaradit 53000, Thailand
Corresponding author, email: [email protected]

Bromelain is a proteolytic enzyme which is prominently found in different parts of pineapple. It has been widely
used in food industry as well as pharmaceutical, leather, and food industries. Nowadays, people are more
interesting to health care and use of natural products for skin care. The purpose of this research was to develop
the skin serum from bromelain extract from Huay Mon pineapple, isolated from each part of Huay Mon
pineapple: core, peel and flesh. The bromelain activities were compared and the results showed that the peel
extract had the highest bromelain activity (p<0.05) with a value of 217.66 + 21.03 CDU/ml, followed by flesh
extract and core extract, respectively. The bromelain extracts from peel have been added into serum skin to
prepare the serum products and compared with the commercial pineapple extracts. The results showed that, 5
kinds of serum formulars had pH ranged from 3.17 + 0.55 to 4.10 + 0.10. Their physical properties were good
stability, clear and homogeneous. The test of satisfaction level revealed that the serum containing 5% (w/w) of
commercial pineapple extract has the highest score of 3.76 + 0.96, followed by the serum containing 5% (w/w)
of bromelain extract with a score of 3.53 + 0.89 that were not significant differences. Therefore, the serum
containing 5% (w/w) of bromelain extract can be developed to the standard products of TISI (Thai Industrial
Standard Institute) certification. The test for TISI certification of facial treatment product was performed. The
results showed that the serum containing 5% (w/w) of bromelain extract had quality standards, with liquid
appearance, clear, colorless, non-precipitate and the contamination of lead (Pb) and microorganisms had not
found.

Keywords: Bromelain, Huay Mon Pineapple, Skin serum

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BIO-P-12 107

Database of plant-growth promoting bioactive compounds in liquid fertilizer from
anaerobic digestion

Kriangsak Anekpong1 and Pitak Sootanan1,*
1 Department of Biochemistry, Faculty of Science, Burapha University
 Corresponding author email: [email protected]

Today, humans have problems with waste management. One of them is organic waste which can be utilized
as a precursor for anaerobic digestion process. These outputs are gas and liquid fertilizers that contained
bioactive compounds or plant hormones that effect the growth of plants. This research aims to study the
functions of these bioactive compounds with database creating to explain the effects of bioactive compounds
in liquid fertilizers on plants. By searching and selecting information from public databases to analyze
relationships, plan and design an E-R model before database creating with program Microsoft Access. The
results show these bioactive compounds are associated with metabolic processes that effect plant structures,
for example, Indole-3-acetic acid improves seedling development, increase fruit and seed size; benzoic acid
improves root elongation; and 4-hydroxybenzoic acid improves durability. There are consistent with the effect
of these bioactive compounds on plant growth that related to amino acid metabolism and salicylic acid
synthesis. In summary, this research will help to provide an overview and to organize the knowledge of the
effects of bioactive compounds on plant growth for the further studies and applications.
Keywords: Bioactive compound, Organic waste, Liquid fertilizer, Database

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BIO-P-13 108

Database of Bioactive Compounds in Durian

Chotika Yodchun1 and Pitak Sootanan1*
1 Department of Biochemistry, Faculty of Science, Burapha University
 Corresponding author, email: [email protected]

Durian is a fruit with a unique taste and is considered beneficial for health due to its bioactive ingredients. It
also has been dubbed as the “Fruit King”, which has been studied and reported in research articles
continuously. This research aims to design and develop a database based on the bioactive compounds obtained
from research articles related to studies in durian. Chemical-protein interactions of each bioactive compounds
are retrieved from public database to explain their actions at the molecular level. Data collection, on-demand
data analysis, conceptual design, logical design and form creating for reporting results are five steps in this
methodology. Data are collected from 6 species of durian, namely Monthong, Chanee, Kan Yao, Kradum,
Puang Manee, and Kop Takham. A conceptual design database has a total of 17 entities, including 39 attributes
containing 5 groups of 24 bioactive compounds. Each strain of durian containes different kinds and amounts
of bioactive compounds. For example carotenoids are high in and Chanee and Monthong, while flavanone is
high in Kradum and their activities are related to the molecular function on health benefits such as anti-
inflammation and anti-oxidation. Therefore, the researcher sincerely hopes that the database preparation and
design of the database from this research article will be the starting point for further development for the benefit
of those interested in further.
Keywords: Durian, Bioactive compound, Interaction Data, Database

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-16 111

Preliminary comparative study of shell, radula and genital system of terrestrial microsnails
genus Gyliotrachela Tomlin, 1930 and Anauchen Pilsbry, 1917 from Eastern Thailand

Onchira Saenkamon1, Noppadon Wiboonpuech1, Sasicha Techama1, Kitti Tanmuangpak2 and Pongrat Dumrongrojwattana1,*
1 Department of Biology, Faculty of Science, Burapha University. Bangsaen, Chonburi 20131
2 Program of Biology, Department of Science, Faculty of Science and Technology, Loei Rajabhat University, Loei 42000
Thailand
* Corresponding author email: [email protected]

Shell, radula and genital system in 2 genera and 2 microsnail species, Gyliotrachela diarmaidi and Anauchen
srakeoensis were examined for taxonomic investigation. The results show that radula morphology are
polyglossate type while shell morphology and genital system are different.
Keywords: microsnails, radula, shell morphology, Gyliotrachela

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วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-17 112

A cross-sectional study of trematode metacercariae in cyprinid fish from Ubolratana
reservoir, Khon Kaen province, Thailand

Nipawan Labbunruang* Jeerawat Boonyai and Rawiwan Wakchaisong
Department of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani, 41000
* Corresponding author email: [email protected]

Freshwater fish are the second intermediate host of liver flukes and intestinal flukes. Parasites are transmitted
to humans through the consumption of traditional dishes cooked with raw Cyprinoid fish. In our study, the
prevalence of trematode metacercariae in Cyprinoid fish was investigated. A total of 154 specimens of two
cyprinid fish species, Henicorhynchus siamensis (n=112) and Puntioplites proctozystron (n=42) were obtained
from Ubolratana Reservoir in Khon Kaen Province, Thailand, during July to October 2018. The samples were
digested in a 0.25% pepsin-HCl solution and filtered through sieves. Metacercariae were observed under stereo
and compound microscopes. H. siamensis was infected with a higher prevalence of metacercariae, 20.53%,
whereas P. proctozystron showed a 9.52% prevalence. A total of 1,506 metacercariae were found. The
trematode species Haplorchis pumilio presented as the largest proportion of total metacercariae (33.06%). This
cross-sectional study indicates that the residents in this area have a likelihood of parasite infection. However,
larger sample sizes and longer study times are suggested.
Keywords: Metacercariae, Cyprinoid fish

การประชุมวชิ าการระดับชาติ วิทยาศาสตรว์ ิจัย ครั้งที่ 12
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วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-18 113

Development of novel formulation of anesthetic from rosewood (Aniba rosaeodora) for
anesthetizing Nile tilapia (Oreochromis niloticus)

Natnicha Ruenthoy1, Nutthakarn Achapisit1, Nalena Praphairaksit1 and Montree Maneepark1*
1Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110
* Corresponding author, e-mail: [email protected]

Using anesthesia in fish transportation and surgical procedure is an effective way to reduce stress and injuries
before surgery and during transportation in fish. Currently, synthetic chemical such as MS-222 is widely used
as fish anesthesia. However, the residue can be found in fish and can harm the consumers. Therefore, natural
remedies are of interested in substituting chemical anesthesia. In this study, we used rosewood essential oil
(Aniba rosaeodora Ducke) as fish anesthesia due to the property of linalool that has been shown to induce
anesthesia. In addition, the essential oil was developed into the emulsion to increase the water solubility and
effectiveness as a drug carrier in Nile tilapia (Oreochromis niloticus). The results showed the best concentration
of rosewood emulsion for fish transportation was 20 µL/L which can hold the fish at stage 1 anesthesia for 2
hours and the fish recovered after 3 minutes. The best concentration for stage 3 anesthesia to perform surgery
was at 0.15 mL/L, the fish were anesthetized to stage 3 within 3 minutes and recovered within 5 minutes. Thus,
rosewood essential oil emulsion can be used as effective anesthesia in fish with good solubility.
Keywords: rosewood essential oil, fish anesthetic, Nile tilapia

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วนั ที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-P-19 114

The Development of Mugwort (Artemisia vulgaris) Oil as Anesthetizing film for
Transportation of Red Tilapia (Oreochromis niloticus) Juvenile

Narudom Somjing, Peerapat Pimpila and Nalena Praphairaksit*
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Corresponding author email: [email protected]

To minimize the problem of chemical residues from the use of synthetic anesthesia, herbal essential oils are
considered as an interesting alternative fish anesthetic because of their anesthetic efficiency and safety. The
purpose of this research was to develop Mugwort (Artemisia vulgaris) essential oil (MT) as anesthetizing film
for Red tilapia (Oreochromis niloticus) juvenile transportation by evaluating the optimal concentration of MT that
can induce the 3rd stage of anesthesia (less than 5 minutes) and bring the fish back to the recovery stage
within 10 minutes. The results demonstrated that the lowest concentration was 0.6 ml/L which showed
anesthetic potential more than Tricaine Methanesulfonate (MS-222) did significantly (p<0.05). Moreover, there
were no significant differences between the recovery time of MT 0.6 ml/L and MS-222. The results of
anesthetizing film also revealed that the 75 film (F75) which consisted of Sodium alginate 3 g : Glycerol 1 g :
MT 1 ml had the highest water solubility and the optimal concentration for short-time transportation in 1st stage
of anesthesia was 0.5 g/l with a 100% survival rate in both during and after 24 hours of fish transportation.
Furthermore, the water parameters revealed that no significant differences (p<0.05) before and after
experiments. In conclusion, the MT anesthetizing film is effective and safe for employment as an anesthetic for
transporting Red Tilapia.
Keywords: Mugwort oil, Fish anesthetic, Red tilapia ,Anesthetic film

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-20 115

Basic education embryonic development of Buraphana amphipod in Burapha University

Piyaporn Muenrit1 Koraon Wongkamhaeng2 and Pongrat Dumrongrojwattana1,*
1 Department of Biology, Faculty of Science, Burapha University. Bangsaen, Chonburi 20131
2 Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900
* Corresponding author email: [email protected]

Floresorchestia buraphana Wongkamhaeng, Dumrongrojwattana and Pattaratumrong, 2016 was firstly
described in November 2016. The amphipods were commonly found in swamps at Burapha University and
adjacent areas. Further study on the amphipod's postembryonic developments was investigated from February
2020 to January 2021. The sampling was carried out by alcohol trap to explorer. Results of studies on embryo
growth and incubation period Found a female with an egg-carrying bag Carried next to the abdomen The
average number of eggs is 4-10 eggs. The amphipod development was classified into four stages: 1. without
eyes, 2. eyes fully developed, 3. segment developed, 4. subadult. The result revealed that each brood's
embryonic development was not synchronized, which two stages were found in the same brood. The monitoring
on amphipod development is useful as a bioindicator baseline data for Burapha University and adjacent areas.
Keywords: Burapha University, Embryonic development, The burapha amphipod

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วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-21 116

Inhibition effect of Quercetin in pig (Sus domesticus) brain monoamine oxidase enzymes

Piraporn Leungratanamart1, Sopa Ninted2,3 and Songklod Sarapusit2,3,*
1 Chonradsadornumrung School, Chonburi, Thailand, 20000
2 Department of Biochemistry and Research Unit of Natural Bioactive Compounds for Healthcare Products Development,

Faculty of Science, Burapha University, Chonburi, Thailand, 20131
3 Center for Innovation in Chemistry, Faculty of Science, Burapha University, Chonburi, Thailand, 20131
* Corresponding author email: [email protected]

Currently, chronic neurological diseases such as Alzheimer's disease, Parkinson's disease, depression,
attention deficit hyperactivity disorder, and anxiety disorders are problematic worldwide. It has been reported
that one common incidence of these diseases is the improper function of the brain-specific monoamine oxidase
(MAO) isoform A (MAO-A) and B (MAO-B). Excess MAO mediates serotonin and dopamine metabolism leading
to decreased levels of these neurotransmitters. In addition, lipid-peroxidation by the metabolic product of this
neurotransmitter metabolism, hydrogen peroxide (H2O2), was proposed as another factor that could facilitate
the progression of degenerative brain tissue in patients. Recently, pig (Sus domesticus) brain lysate had been
reported to use as an alternative source of MAO enzymes for screening Thai medicinal plants that contain
MAO inhibitor. This study aims to study the inhibition effect of Quercetin, a well-known inhibitor of human MAO
enzymes against pig brain MAO enzymes. The results showed that Quercetin can inhibit pig brain MAO-A with
an IC50 value of 1.95 µg/ml and MAO-B with an IC50 value of 4.96 µg/ml. Comparison of the detail molecular
interaction of Quercetin at the active site of both pig and human MAO enzymes has been under investigation.
Keywords: Monoamine oxidase, Pig brain MAO, Quercetin, Inhibition

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THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-22 117

Using Pineapple Peels in diet on Nile tilapia (Oreochromis niloticus) culture

Jinwara Boonnak1, Prutchayawan Cumthakrue2, Kanta Sangwijit2 and Thanyaporn Tangjaroenchai1,2*
1Demonstration School University of Phayao, Phayao 56000, Thailand
2School of Science, University of Phayao, Phayao 56000, Thailand
*Corresponding author email: [email protected]

Production of feed from agricultural waste Pineapple Peels are used as food for tilapia to reduce costs for
farmers, while in tilapia food there is still value and nutrients that fish should receive by mixing pineapple peels,
soybean meal, rice bran and flour in a ratio of 40:30:20:10 and then compressing pellets with a diameter of 0.3
mm. curing at 60°C for 72 hours and taking granulated food to analyze nitrogen content. The average amount
of nitrogen in proteins is 16 percent. (amount of coarse protein). Compared to granulated food obtained with
commonly sold granulated food. Set up a series of 45-day-old fish experiments, feeding twice a day (morning
and evening). Measure the growth of tilapia from the mouth to the tail and measure water quality every 3 days.
According to this study, this produced can be substituted for fish food sold on the market. And it is information
for those who want to reduce the cost of fish food in the future.
Keywords: pineapple, Nile tilapia, soybean meal, Percentage of Nitrogen, Average of Nitrogen

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วันที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-23 118

Assessment of sediment quality in coastal area, Trat Province

Patrawut Thaipichitburapa1,* and Chalatip Junchompoo2
1Department of Aquatic Science, Faculty of Science, Burapa University, Chonburi Province 20131
2The Marine and Coastal Resources Research Center, the Eastern Upper Gulf of Thailand, Department of Marine and Coastal
Resources, Cha Choeng Sao Province, Thailand 24130
Corresponding author email: [email protected]

The sediment quality assessment survey was conducted in the coastal of Trat Province. The purpose of this
survey was to determine the physical and chemical sediment quality, such as grain size, total organic matter
and acid volatile sulfide. Ten stations were monitored in February (dry season), May (early rainy season) and
August (end of rainy season) in 2018. The results indicated that the most grain size were less than 63
micrometers (48.1 %). The total organic matter between 1.4 to 18.8 %, with the highest in western of Trat Bay.
The acid volatile sulfide was in ranged of 0.0002-0.0299 mg/g dry weight. The sediment quality was significantly
changing according to a spatial pattern (p-value < 0.05). The total organic matter, acid volatile sulfide and grain
size (<63 µm) were significantly positive correlation (p-value < 0.05). the overview of sediment quality of Trat
Bay was abundance, the low sulfide accumulation. Hence, this area was a suitable habitat for marine benthos,
that should be provided a suitable plan for sustainable management in the future.
Keywords: Sediment quality, Coastal area, Trat province

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วนั ท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-24 119

The investigation of storm surge generated by tropical storm Pabuk in the Gulf of Thailand
by using computer modeling technology

Natchanon wasuthawat1, Pakawarat Tiyawalakul1 and Phumirin Issariyakun1,*
1 Piboonbumpen Demonstration School, Burapha University Chon Buri, Thailand, 20131
* Corresponding author’s email: [email protected]

In the past, Thailand was affected by many storms. The effects of storms that frequently occur in Thailand
include floods, strong wind, and storm surge. In 2019 December, Thailand was affected by the storm surge
from Tropical Storm Pabuk that formed in South China Sea. Consequently, the objective of this study was to
create a model that presents the water elevation and circulation by using computer modeling technology,
Advance Circulation Model (ADCIRC). The map, water, and wind information were gathered from the public
database. The model was created by data importing for 21 days during the storm to Thailand. After that, the
exported data could plot graphs and compare with the graphs that plot from measured data. The results
obtained from running the model are close to the actual measured values (Standard Error of the Estimation of
5 stations were 0.296, 0.306, 0.540, 0.492 and 0.310). This showed that the Tropical Storm Pabuk has affected
most of the southern and central Gulf of Thailand, and the water level during the storm blowing onto the coast
was higher than normal water level. Examples of impacts included heavy rain, strong wind and high wave that
damaged the coastal livelihoods and other. The data obtained from this model can be used to find the
prevention and decrease of the storm surge impact in the future.
Keywords: Storm Surge, Tropical Storm Pabuk, ADCIRC

การประชุมวชิ าการระดับชาติ วิทยาศาสตร์วจิ ัย ครั้งที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-P-25 120

Diversity of Fireflies in The University of Phayao

Panupong Chaiwongsaen1,*, Kraisorn Jongnan1, Jakrin Nenjatee1, Wiwat Tharin1 and Sorasak Nak-eiam2
1Department of Biology, School of Science, University of Phayao, Phayao, 56000
2Department of Biology, Faculty of Science and Technology, Rambhai Barni Rajabhat University, Chanthaburi, 22000
*Panupong chaiwongsaen email: [email protected]

The University of Phayao has a mostly dry evergreen forest. And many natural reservoirs Cause the biodiversity
of living things It is also seen that many adult fireflies fly in the natural reservoir. At present, the source of
fireflies has been destroyed and changed. Making it less likely to find fireflies, Therefore, a study of the diversity
of fireflies in the area of the University of Phayao. The objective is to study the diversity of fireflies. Study
physical factors And biological effects on diversity in Phayao University. Operated between January 2020 and
January 2021. Random sampling along with the line transect distance of 100 meters from 4 different
environmental habitats such as 1) Water reservoir (Faculty of Architecture) 2) Water reservoir (Ang-Luang) 3)
Water reservoir (Water supply plant) and 4) Water reservoir (Huai Thap Chang). The results samples of 82
adult fireflies were collected and revealed that all fireflies were classified into the subfamily Luciolinae and
consisted of 4 genera. These identified fireflies were Asymmetricata ( 3 individuals) , Luciola ( 2
individuals) , Pyrocoelia ( 1 individual) , and Sclerotia ( 76 individuals) . The most common type of collected
species in all habitats was Sclerotia aquatilis ( 76 individuals: 92. 77% ) , Asymmetricata circumdata ( 3
individuals: 3.61%), Pyrocoelia praetexta (1 individual: 1.20%), and Luciola curtiothorax (2 individuals: 2.41%).
The station that found the most adult fireflies was Huai Thap Chang Station ( 76 individuals: 92. 68% ) . The
station with the most diversity found was the water reservoir ( Ang- Luang) ( H’ 1. 24) . The evaluations of the
fireflies diversity, physical factors, and biological factors indicated that the natural habitats such as water
reservoirs (Ang-Luang) were suitable for fireflies diversity and abundance. Found that the Ang Luang Reservoir
area is suitable for various types And the abundance of fireflies It was found that the overall species diversity
index, richness index, evenness index, and species abundance were 1.24, 1.67, 0.69 and 16.67, respectively.
It can be concluded that the diversity and abundance of fireflies were found in a natural habitat where the
environmental quality was fairly good. The three important fireflies species i. e. Asymmetricata
circumdata, Lucioila curtiothorax, and Pyrocoelia praetexta can be used as a bioindicator for environmental
monitoring at the University of Phayao.

Keywords: Fireflies, Diversity, University, Phayao

การประชุมวิชาการระดับชาติ วิทยาศาสตรว์ จิ ัย คร้ังท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-26 121

Diversity of zooxanthellae in the family Symbiodiniacaea in four coral taxa (Acropora,
Dipsastraea, Platygyra and Pocillopora) from inshore reef in Samaesarn Subdistrict,
Sattahip District, Chon Buri Province

Kannaphat Wongprates1, Kanyawee Srisungngam1, Em-on Klanyuang1 and Narinratana Kongjandtre2,*
1 Science Classrooms in University-Affiliated School Project, Piboonbumpen Demonstration School, Burapha University,
Chonburi, Thailand, 20131
2 Department of Aquatic Science, Faculty of Science, Burapha University, Chonburi, Thailand, 20131
* Corresponding author’s email: [email protected]

Diversity of zooxanthellae in the family Symbiodiniaceae (Dinoflagellata) from inshore reef at Samaesarn
Subdistrict, Sattahip District, Chon Buri Province was conducted on November 14, 2020. Five specimens
were randomly sampled from each coral taxa namely, Acropora sp., Dipsastraea spp. (formerly known as
Favia), Platygyra sinensis and Pocillopora damicornis. To resolve polymorphisms, Denaturing Gradient Gel
Electrophoresis (DGGE) was used for the Internal Transcribed Spacer-2 (ITS2) DNA. DGGE profile revealed
3 zooxanthellae species (C3u, D1 and D6) belonging to two genera (Cladocopium and Durusdinium) in the
family Symbiodiniaceae. DNA sequencing confirmed that Cladocopium (C3u) was found in Acropora and
Dipsastrea and at least one Durusdinium species (D1 and D6) was found in Pocillopora damicornis where
as in Platygyra only found Durusdinium (D1). There were two difference DGGE profiles of Durusdinium (D1
and D6). However, it cannot be fully resolved by the ITS2 marker. The ITS2 could only provide partial insight,
as D. glynnii (D1, D1-6) and D. trenchii (D1-4) contained some shared haplotypes, i.e., D1-4-6. Thus the
delineation of very closely related Durusdinium species must be confirmed using high resolution genetic
markers.

Keywords: Cladocopium, Coral reef, Durusdinium, Gulf of Thailand, Symbiodiniaceae

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วิจัย คร้ังท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-27 122

Geographical distribution and the resting sites of Aedes albopictus for the focal control

Ekarat Denchonchai1,*, Wannisa Suebsaard1, Chitti Chansang1 and Uruyakorn Chansang1
1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, 11000
* Corresponding author’s email: [email protected]

Aedes albopictus, an Asian tiger mosquito, is an epidemiologically important vector of Chikungunya fever in
Thailand. There were 5,728 case report in 2020. According to the lack of information on complete report of
geographical distribution and resting site for the efficient focal mosquito control, this study was performed to
obtain these data. Two study areas comprised of rubber plantations and other different cohorts in Nong Sua
Chang of Ban Bueng and Phuong Thong of Bo Thong in Chonburi province were selected based on the
geographical information. The adult mosquitoes were surveyed and sampling by the questing and hovering
techniques with the appropriate developed tools. Four Genus and eight species of mosquitoes were found.
Among these, 80 % and 90% of mosquitoes were Ae. albopictus which the captured rate was 20/hour and
24.5/hour, respectively. The developed resting boxes and new efficient mosquito suction were used to
investigate the resting sites of Ae. albopictus in the high density areas of mosquitoes in Chonburi province.
The mosquitoes were more abundant in the rubber plantation with bushes than in the single rubber trees. The
environmental conditions were noted and then were analyzed together with the data of mosquitoes. All the
information was presented as GIS map showed the density of the Ae. albopictus during 6.00AM – 9.00 PM
compared among the 3 areaes of residences, rubber plantations and forest in Chonburi province. These results
could be used for the focal control of Chikungunya fever in Thailand.
Keywords: Aedes albopictus, Geographical distribution, Resting site, Focal control

การประชุมวิชาการระดับชาติ วิทยาศาสตร์วิจัย ครัง้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-P-28 123

Aedes albopictus entomological index: the geographical feature of the dengue fever
distribution in Nakhon Si Thammarat Province

Puttisan Rattanachoo1 Phimwimon Srisuksai2 and Siriluk Sintupachee1,*
1 Program in Creative Innovation in Science and Technology, Faculty of Science and Technology, Nakhon Si Thammarat
Rajaphat University, Nakhon Si Thammarat, 80280
2 Program in Biology, Faculty of Science and Technology, Nakhon Si Thammarat, 80280
* Corresponding author email: [email protected]

Nakhon Si Thammarat is a geographically diverse province, the natural ecosystem characteristics would be
divided into mountains, fields, forests, and seas resulting in a wide variety of natural resources, both flora, and
fauna. Climate is suitable for the growth and spread of both vector-borne disease and various pathogens, that
have reported various summer outbreaks. Due to mosquito-borne diseases, Nakhon Si Thammarat was found
that the highest outbreak of dengue fever is increasing every year. So, this study was aimed to investigate the
mosquito-vector population using an entomological index according to the geographical characteristics as a
mountain range (center area), coastal plains (eastern area), and lowland (western area) (Nakorn Si Thammarat
zoning classification for geologic and mineral resources management, Department of Mineral Resources,
Ministry of Natural Resources and Environment) from February to December 2020. We used the human-baited
collection method and taxonomy to identify the mosquito vectors and analyzed the correlation between the
vector number with time and duration of vector breeding, the weather, and geography for the basic prevention.
Most of the mosquito collection was Ae. albopictus species (38990/40982 adults). The Ae. albopictus vector
found 71.17, 4.45, 23.36% on the coast of the Gulf of Thailand (Pak Panang and Kanom district), at the forest
area (Promkiri and Thungsong district), and in the various orchards (Thung Yai, Thungsong, Promkiri, and
Meang), respectively. The gender of the Ae. albopictus was 82.45 and 17.55% for females and males,
respectively. The number of the mosquito-vector was abundant in April and May. The seasonal distribution of
the Ae. Albopictus population correlated with the dengue cases reported by the Nakhon Si Thammarat
Provincial Public Health Office.

Keywords: mosquito, dengue fever, entomology index, Aedes albopictus, geographical feature

การประชุมวชิ าการระดับชาติ วทิ ยาศาสตรว์ ิจัย คร้ังที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-P-29 124

Morphology and nutrition of Diaphanosoma sp. (Diplostraca: Sididae) collected from
Wonnapa Beach, Chon Buri Province

Penpitcha Srinui1,* Khwanruan Srinui2 Nisa Siranonthana2 and Jarunan Pratoomyot2
1 Piboonbumpen Demonstration School, Burapha University, Chon Buri, Thailand, 20131
2 Institute of Marine Science, Burapha University, Chon Buri, Thailand, 20131
* Corresponding author email: [email protected]

Specimens of the cladoceran Diaphanosoma were collected by plankton net (mesh size 100 µm) from the
brackish waters of Wonnapa Beach, Chon Buri Province in August 2019. The samples were cultured in 20 ppt
saline, 100 L tanks at the Institute of Marine Science, Burapha University and fed the microalgae Tetraselmis
sp. Morphology of Diaphanosoma sp. was examined macro- and microscopically (n=16). It has an oval body,
round head, large compound eyes positioned on the dorso-anterior. The carapace lacks spines; antennules
are short; swimming antennae represent >60% of the flea’s length, extending to the posterior valve margin.
Diaphanosoma sp. possesses massive basipodites armed with a small spine on the outer apical segment; the
upper and lower branch have setae formula 4-7/0-1-4 respectively. Posterior margin of the abdomen has two
terminal claws with three basal spines, and two long post-abdominal setae. Diaphanosoma sp. found in this
study is morphologically similar to D. celebensis. Species of this genus have not been previously recorded from
Thai marine waters and this species maybe new record to Thailand; molecular analyses are required to
investigate. To study their nutrition and chemical composition, proximate analysis was performed and revealed
that specimens contain 56.44±0.10% crude protein, 18.55±0.33% NFE, 0.04±0.01% fiber and 11.33±0.17%
crude lipid. This nutritional composition is similar to that of the genus Artemia – which is a key anostracan
species for feeding young aquatic animals in aquaculture. Diaphanosoma sp. may represent an alternative
species for mass culture and further industrial applications.

Keywords: Diaphanosoma, Cladocera, Morphology, Nutrition, Wonnapa Beach

การประชมุ วิชาการระดับชาติ วิทยาศาสตรว์ ิจัย ครั้งท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-P-30 125

Response of sap flow on size and height of Dipterocarpus tuberculatus Roxb. in dry
dipterocarp forest, University of Phayao

Jidsupa Utthiya1, Papichaya Yossala1, Montri Sanwangsri2 and Pimsiri Suwannapat3,*
1 Demonstration School University of Phayao University of Phayao, Phayao, Thailand, 56000
2 Department of Agriculture and Natural Resources Chiang Mai University, Chiang Mai, Thailand, 50200
3 Faculty of Energy and Environment University of Phayao, Phayao, Thailand, 56000
* Corresponding author’s email: [email protected]

This research studied sap flow, in Dipterocarpus tuberculatus Roxb., which was a dominant species in dry
dipterocarp forest using Thermal Dissipation Probe, Granier-Type (TDP), which was measured on 6 samples
in the same site. The research was done between January to September in 2020. The result reveals that the
sap flow pattern slightly rose from 7:00 a.m. and highest during 11:00-11:30 a.m., then decreased at noon and
returned higher during 2:30-4:00 p.m. before dropped in the evening to night and shows the tree No.1 gave
the most sap flow corresponding to the biggest and tallest of the trunk than the other trees. Therefore, the
influence of internal factors such as sapwood area and trunk height on sap flow was studied. Analysis of the
relationship of trunk height and sapwood area size on sap flow illustrated polynomial relationships. In summary,
the sap flow was risen more 200 millimeter, an the height of trees would be slightly decreased and the sap
flow a value of 250 millimeter or more could give sapwood area would be risen remain constant, respectively.
This result could be applied to estimate water balance in the forest ecosystem. However, other species should
be considered to understand mechanism of transpiration in dry dipterocarp forest.

Keywords: Dipterocarpus tuberculatus Roxb., Dipterocarpaceae, Thermal Dissipation Probe, Sapwood size,
Dry dipterocarp forest

BIO-P-32 127

Antibacterial and antioxidant activities of extracts from Tamarindus indica L. and
Cucurbita moschata

Pimmada Maneejiraprakarn 1 Krin Somwandee2 and Pichaya Chowtivannakul3*
1,2,3 Naresuan University Secondary Demonstration School, Faculty of Education 65000
*Corresponding author e-mail: [email protected]

This research aimed to examine anti-bacterial activities on Staphylococcus aureus, Escherichia coli and the
antioxidant activity of Phetchabun tamarind seed extract and Toad – pumpkin seed extract. Our project team
extracted the essential substances from Phetchabun tamarind seeds and Toad pumpkin seeds using 95%
Ethanol and Hexane. The test for inhibition of S. aureus and E. coli was observed in the plate by measuring
the radius of clear zone. Antioxidant activity was tested by FRAP and DPPH methods. The study found that
the Phetchabun-tamarind seed extract is able to inhibit the growth of S. aureus and E. coli with the average
radius of clear zone 4.7 mm. On the other hand, the Toad pumpkin seed extract has no changed. The
Phetchabun-tamarind seed extract show antioxidant activities by DPPH method (DPPH mean 0.04±0.00) while
Toad pumpkin seed extract showed no change. The antioxidant activities of Phetchabun tamarind seed extract
and Toad-pumpkin seed extract by FRAP method showed the mean 6247.94±83.34 and 0 . 3 8 ±0 . 0 3 ,
respectively. It can be concluded that Phetchabun tamarind seed extract was able to inhibit E. coli and
S. aureus and antioxidant better than the Toad-pumpkin extract.
Keywords: bacterial activities , antioxidant , Ethanol, Hexane

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วิจัย คร้งั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-P-33 128

Development of microbial products of antagonistic yeast Pichia kudriavzevii VCU24 in
liquid formulations for controlling anthracnose disease in mango

Anuthep Pasura1,* and Salinee Udomsuk1
1Department of Microbiology, Faculty of Science, Burapha University, Chonburi, 20131
*Corresponding author email: [email protected]

Anthracnose disease caused by Colletotrichum gloeosporioides is a major problem which affects the damage
of post-harvested mango. Using chemical fungicides in controlling this disease has disadvantages and
limitations. Therefore, biological control becomes an alternative method for reducing the use of chemical
fungicides. This study aims to develop microbial products of Pichia kudriavzevii VCU24 in liquid formulations.
The P. kudriavzevii VCU24 cells were grown in Nutrient Yeast Dextrose Broth with 5% glycerol was performed
to modify water activity of the culture medium. Shelf-life of the microbial product of P. kudriavzevii VCU24 in
liquid formulations and efficacy of microbial products in controlling anthracnose disease in mango were
evaluated. The results showed that the shelf-life of P. kudriavzevii VCU24 in liquid formulations with maltose
concentrations of 1, 2.5, 5 and 10% and stored at 4±2˚C for 90 days had cell reduction at 0.57, 0.70, 0.48 and
0.52 Log cfu/ml, respectively. Viable numbers of yeast cells of the microbial product with the same maltose
concentrations and stored at 26±2˚C for 42 days had higher cell reduction at 1.38, 1.13, 0.92 and 0.81 Log
cfu/ml, respectively. This microbial product of P. kudriavzevii VCU24 in liquid formulation could reduce
anthracnose disease severity on mango at 66.28% which was not significantly difference from using fresh yeast
cells.

Keywords: Anthracnose disease, Biological control, Maltose, Colletotrichum gloeosporioides, Pichia
kudriavzevii

การประชมุ วิชาการระดับชาติ วิทยาศาสตร์วิจัย ครง้ั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-P-34 129

Diversity of Lichen at Ban Na Ta Rot Community Forest, Hat Ngiu Sub-District,
Muang District, Uttaradit Province

Jurarak Pankeat1 Parinya Pimpila1 Sirimon Chaiya1 and Kodchakorn Lapmak 2,*
1 Program of Biology, Faculty of Education, Uttaradit Rajabhat University, Uttaradit, 53000
2 Program of Biology, Faculty of Science and Technology, Uttaradit Rajabhat University, Uttaradit, 53000
* Corresponding author email: [email protected]

The objective of this research was studied diversity of lichen at Ban Na Ta Rot community forest, Hat Ngiu
sub-District, Muang District, Uttaradit Province during July to September 2020. Epiphytic lichens at the way
up to the Mon Phra Buddhabart Si Roy temple area was surveyed by using 100 x 100 m plots size. The
lichen were collected from sampling trees with circumference size 35-50 cm. All lichen specimens were
identified on the basis of morphological characteristic of fungus and chemical test (spot test) on thallus.
Results of the survey revealed 29 lichen taxa belonging to 9 genera, 8 families. The lichen were divided in
to 2 groups including crustose lichen which is comprised of 28 taxa, 8 genera and 7 families and foliose
lichen that comprised of 1 taxa, 1 genus and 1 family. The most lichen diversity were found in family was
Graphidaceae comprised of 4 genera, 17 taxa. Diversity index (H’) was 2.55. The results of this research
can be sued as a biodiversity information for conservation planning and utilization in the future.
Keywords: Diversity, Lichen, Community forest, Taxonomy

การประชุมวิชาการระดับชาติ วิทยาศาสตร์วจิ ัย ครงั้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-35 130

Species Diversity of Mushrooms and Macrofungi at Ban Na Ta Rot Community Forest,
Hat Ngiu Sub District, Muang District, Uttaradit Province

Nirattisai Nantachai1 Kanjana Tikaew1 Rattanawadee Buakla1 and Kodchakorn Lapmak2,*
1 Program of Biology, Faculty of Education, Uttaradit Rajabhat University, Uttaradit, 53000
2 Program of Biology, Faculty of Science and Technology, Uttaradit Rajabhat University, Uttaradit, 53000
* Corresponding author email: [email protected]

The objective of this research is the study of species diversity of mushrooms and macrofungi at Ban Na Ta Rot
community forest, Hat Ngiu Sub District, Muang District, Uttaradit Province. The samples of mushrooms and
macrofungi were collected during July to September 2020. All of samples were identified based on
morphological characteristics. Results of the survey revealed 67 samples of mushrooms and macrofungi
belonging to 50 species, 29 genera, 19 families and 6 orders. The most common family was Marasmiaceae
comprising 10 species followed by Russulaceae comprising 6 species. Diversity index (Shannon - Weiner’s
index of diversity; H') is 3.59. All of mushrooms and macrofungi were able to identify their roles in the
ecosystem into four groups, including, 32 species of saprophytic mushroom, 15 species of ectomycorrhizal
mushroom, 2 species of termite mushroom and 1 species of parasitic mushroom. The results of this study
showed that Ban Na Ta Rot community forest was still fertile. It was suitable for the growth of most
mushrooms and macrofungi that play role as saprophytes and ectomycorrhiza. The information of number
and species of mushrooms and macrofungi that found in this study can be used for planning of conservation
and utilization in the future.
Keywords: Diversity, Mushrooms, Macrofungi, Community Forest

การประชมุ วิชาการระดับชาติ วิทยาศาสตรว์ จิ ัย ครง้ั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

BIO-P-36 131

Leaf and stem anatomy of three Tradescantia L. species in Thailand

Kanyakorn Pekkrue1 and Boonchuang Boonsuk2,*
1 Demonstration School, University of Phayao, Phayao, Thailand, 56000
2 Department of Biology, School of Science, University of Phayao, Phayao, Thailand, 56000
* Corresponding author’s email: [email protected]

The genus Tradescantia L. belongs to the family Commelinaceae, introduced to Thailand for ornamental
plants. The aims of this study are to investigate the anatomical characters of stems and leaves including
leaf surfaces of three Tradescantia species occurring in Thailand. The transverse section of stems and
leaves were performed by paraffin method and stained with 1.2% (w/v) Toluidine Blue in water. The leaf
epidermis was studied using peeling method and stained with 0.5% (w/v) Safranin O in 50% ethanol. Samples
in permanent slides were observed under light microscopy and photographed. The results reveal that bifacial
leaves with tetracytic stomata on the lower surface, round stem comprising a single epidermis layer covered
with a thin cuticle, pericycular vascular bundles and vascular bundles in ground tissue, and calcium carbonate
crystals in both leaves and stems are present in all plants studied. The taxonomically useful characters for
identification could be recognized as follows: presence and size of hypodermis, thickness of mesophyll, layer
of collenchymatous and parenchymatous cortex, and numbers of pericycular vascular bundles and vascular
bundles in ground tissue.
Keywords: Commelinaceae, Rpidermis, Hypodermis, Monocots, Transverse section

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วิจัย ครงั้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-37 132

Potential of Local Plant Extracts in Uttaradit Province Against Pathogenic Fungi of Root
and Stem Rot Disease and Pink Disease of Durian

Wanwisa Pirapak*,1, Nattharintr Pattama1 and Dararat Yuhang1
1 Program of Biology, Faculty of Science and Technology, Uttaradit Rajabhat University, 27 Injaimee road, T.Tha-it, A. Mueang,
Uttaradit Province, 53000
* Corresponding author email: [email protected]

Root and stem rot disease and pink disease is important disease in the durian production. In this study, root
and stem rot disease and pink disease of durian in Uttaradit province was investigated. Three isolates of root
and stem rot disease pathogens and 2 isolates of pink disease pathogens were isolated and identified as
Phytophthora spp. and Erythricium spp. Pathogenicity test of those pathogen showed that the highest level of
pathogenicity test was found in Phytophthora sp. isolate P02 and Erythricium sp. isolate UT02. The 7 local
plant extracts against fungal pathogens were tested by Poisoned Food Technique at 10,000 ppm of extract
concentration. The results indicate that all local plant extracts possessed significant antifungal activity against
all two isolates of fungi. The 95% ethanal ginger rhizome extract present the most effectiveness on the growth
inhibition of Phytophthora sp. isolate P02 followed by 95% ethanal fingerroot extract and 47.5% ethanal ginger
rhizome extract, respectively. Erythricium sp. isolate UT02 was growth inhibited by 95% ethanal mangosteen
pericarp extract and 95% ethanal ginger rhizome extract followed by 95% ethanal fingerroot extract, 47.5%
ethanal mangosteen pericarp extract and 95% ethanal turmeric extract, respectively. This research was
presented that ginger rhizome and mangosteen pericarp have efficiency for control root and stem rot disease
and pink disease in durian. It is interesting that local plant extracts are reported for the first time as an effective
agent against a pathogen of pink disease.

Keywords: Plant Extracts, Root and Stem Rot Disease, Pink Disease, Durian

การประชุมวิชาการระดับชาติ วิทยาศาสตร์วจิ ัย ครัง้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

BIO-P-38 133

Antimicrobial activities of a novel type-I crustin (Lvcarcinin) from whiteleg shrimp
(Litopenaeus vannamei)

Thassanee Srisook,1,* Suwattana Visetnan 2 and Suchao Donpudsa 1
1 Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand
2 Faculty of Dentistry, BangkokThonburi University, Bangkok 10170, Thailand
* Corresponding author email: [email protected]

Crustins were antimicrobial peptides that were found in shrimp immune system. Their biological activities
exhibited the growth inhibition of several microorganisms. In previous research of type-I crustin, it showed the
antimicrobial activities against Gram-positive bacteria, Gram-negative bacteria, fungi and virus. In this work,
a novel type-I crustin (Lvcarcinin) from the whiteleg shrimp (Litopenaeus vannamei) was studied.
The Lvcarcinin consists of an open reading frame of 381 bp encoding a putative protein of 126 amino acid
residues. The recombinant Lvcarcinin protein was produced in the Escherichia coli expression system
in order to characterize the antimicrobial activities using agar diffusion assay. The assay showed that
the growth of Bacillus subtilis, was inhibited with the minimum inhibitory concentration of about 50 µM. So, the
Lvcarcinin was probably involved in the shrimp immune system.
Keywords: Litopenaeus vannamei, Type-I crustin, Recombinant protein, Antimicrobial activities

การประชุมวิชาการระดับชาติ วทิ ยาศาสตรว์ จิ ัย ครั้งท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

Poster Presentation

กล่มุ ที่ 2
สาขาวิชาเคมี เคมปี ระยุกต์ และเคมีส่ิงแวดล้อม

การประชุมวิชาการระดับชาติ วิทยาศาสตรว์ จิ ยั ครัง้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวทิ ยาลยั นเรศวร

CHEM-P-01 134

Development of Cathode Catalyst for Glucose Alkaline Fuel Cell

Sitanan Bunyord1 and Chakkrapong Chaiburi1,*
1 Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung campus, Phatthalung, 93210, Thailand
* Corresponding author: e-mail: [email protected]

The aim of this research is to analyze the efficiency of two different catalysts for the reduction reaction of
glucose in alkaline fuel cells. The results found that Ni/C and Bi/C catalysts exhibited smooth surface
morphology and a good distribution of metal on the supporter. Herein, a comparison of the efficiency of both
catalysts on the electrochemical characteristics of the alkaline fuel reduction reaction using glucose as fuel is
provided. The ratio of glucose concentrations of 0.1, 0.2, 0.3, 0.4 and 0.5 M together with 0.1 M alkaline KOH
solution was analyzed by the cyclic voltammetry technique. The Bi/C catalyst showed a higher reduction
reaction of glucose than the Ni/C catalyst. The Bi/C catalyst exhibited a reduction reaction at a potential at -
0.95 V and gave a maximum current density of -1.00 mA/cm2 at the concentration of 0.1 M of glucose. When
glucose is added to an electrolyte of 0.1 M of KOH, it indicates resistance to oxidation conditions for use at the
cathode electrode.
Keywords: Alkaline Fuel Cells, Glucose, Reduction Reaction, Cathode Electrode

การประชมุ วชิ าการระดับชาติ วทิ ยาศาสตร์วจิ ัย คร้งั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-P-02 135

A facile synthesis and characterisation of CaO/Ca2Fe2O5 nanocomposite from eggshell
waste

Ajchara Imkum Putkham1,* and Pimpisa Japrasong1
1Department of Chemistry, Faculty of Science, Naresuan University, Phitsanuloke, 65000
* Corresponding author email: [email protected]

There are a lot of eggshell waste from industrial and household. In the get rid of the eggshell waste, it is done
by sending for disposal of the waste and this elimination method has cost expensive. This research was
developed by using eggshell waste and calcium oxide ( calcined at 900 ºC) as precursor and studied the co-
precipitation method with ferrous sulfate solution for facile synthesis of CaO/ Ca2Fe2O5 nanocomposite. In
addition, the products were characterized physically and chemistry by various techniques such as X- ray
diffractometer ( XRD) for calcium oxide structure analysis, Fourier transform infrared spectrometer ( FTIR) for
functional group analysis, scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) for
morphology and element analysis, as well as Hammett indicator and titration for basic strength analysis of
calcium oxide catalyst. It was found that the porous structure of product has calcium and iron as the main
component which iron is adhered to the calcium surface within the pores of structure for nanocomposite
synthesis. Therefore, this method is a simple, low cost for using eggshell as precursor and as well as
environmentally friendly, but also adds value to the inorganic waste.
Keywords: calcium oxide, eggshell, nanocomposite

การประชมุ วชิ าการระดับชาติ วทิ ยาศาสตร์วิจัย ครง้ั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-P-03 136

Preparation of slow-release fertilizers of zinc and ammonium ions on zeolite A

Sirinuch Loiha 1,* Kridsadakorn Autaros1, Pitak Nasomjai2, and Nichapha Senamart1
1 Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002
2 Department of Chemistry, School of Science, University of Phayao, 56000
* Corresponding author email: [email protected]

Zeolite A is an aluminosilicate structure with high surface area and porosity which can be applied as fertilizer
for holding the nutrients and water in the root zone until the plant is ready to utilize them. In this work, sodium
form of zeolite A (NaA) is synthesized via hydrothermal method and ion exchange to ammonium form (NH4A)
in ammonium nitrate solution. Zinc nanoparticles are precipitated using KOH solution on both forms of zeolite
A namely Zn/NaA and Zn/NH4A to form the zeolite fertilizers. Crystal structures and morphology of the fertilizers
are investigated by XRD, FT-IR and FIB-SEM EDS techniques. The Zn/NaA and Zn/NH4A fertilizers show the
crystalline zeolite A containing ZnO nanoparticles. A mixture of zeolite fertilizers and soil shows an improvement
of holding moisture in comparison to bare soil. Slow-released fertilizers of Zn/NaA and Zn/NH4A are investigated
in power and granular forms. Maximum amounts of about 20 and 22% of Zn2+ ion are released from Zn/NaA
and Zn/NH4A powder, respectively, within 7 days and constant for 45 days. The granule fertilizers are prepared
by suspension of zeolite fertilizers in chitosan solution. Very slow released of Zn2+ from the granule fertilizers
in a maximum amount of 2 and 3% in 7 days for Zn/NaA and Zn/NH4A, respectively, are determined. In addition,
a macronutrient of nitrogen in NH4+ form is efficiently investigated on Zn/NH4A fertilizer. Both Zn2+ and NH4+
are mostly accumulated in plant roots which are sufficient for plant growth for 45 days.
Keywords: Fertilizer, ZnO nanoparticle, nitrogen, micronutrient

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วจิ ัย คร้ังท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-P-04 137

Efficient Removal of Hydrogen Sulfide in Biogas using Water Mimosa biochar as an
adsorbent

Tantika Charoenlap1, Usa Onthong1, Niramol Juntarachat*1
1Department of Chemistry, Faculty of Science, Thaksin University, Phattalung campus, Phattalung 93210
Corresponding author, email: [email protected]

In this work, the removal efficiency of hydrogen sulfide from biogas using agricultural waste: water mimosa as
an adsorbent was studied. Firstly, water mimosa biochar was produced though pyrolysis process. Physical and
chemical properties of biochar was determined. Moisture content, volatile matter content, ash content, and fixed
carbon were found to be 4.99±1.02, 33.37±1.93, 36.81±3.70 and 24.93 %, respectively. The pH value of biochar
was 7.47±0.53 and the surface area of the water mimosa biochar was 1.63 m2/g. Secondly, the H2S removal
efficiency of water mimosa biochar adsorbent was studied at different pellet sizes: 0.5 cm and 1.0 cm with
initial concentration of H2S at 900 ppm, adsorbent mass of 100 gram and biogas flowrate of 1.5 mL/min. The
concentration of H2S before and after the adsorption process was analyzed by a mobile gas chromatography.
The results showed that adsorbent of diameter of 0.5 cm and 1.0 cm had the adsorption efficiency, at 120 min,
of 0.84% and 0.38%, respectively. A small pellet size increases surface area of adsorbent, allowing the increase
of the adsorption capacity. Water mimosa biochar could be an alternative adsorbent for removing H2S from
biogas but other treatment such as alkaline impregnation is necessary
Keywords: Biogas, Adsorption efficiency, Hydrogen Sulfide, Biochar, Water mimosa

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วจิ ัย ครัง้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-P-05 138

Electrochemical of Alloy Catalysts for Cathode Electrode of Reducing Sugar Alkaline Fuel
Cells

Kantrakorn Suwanraksa1, Piyawat Masjod1, and Chakkrapong Chaiburi1,*

1 Department of Chemistry, Faculty of Science, Thaksin University, Papayom district, Phatthalung, 93210 Thailand.
*Corresponding author, e-mail : [email protected], [email protected]

This study investigated the electrochemistry of two alloy catalysts, AgVxOy/C and AgMnxOy/C. The objective of
this work was to analyse the potential use of catalysts in cathode electrodes for reducing sugar in alkaline fuel
cell without an exchange membrane. The physical properties of the catalysts were investigated by scanning
electron microscopy, and the quantity of elements in the catalysts was determined by energy dispersive x-ray
spectroscopy. The electrochemical characteristics of the catalytic reduction reaction were measured by the
cyclic voltammetry technique. It is found that the AgMnxOy/C catalyst had better catalytic activity than the
AgVxOy/C catalyst in higher fuel concentrations. The maximum current density of the reduction peak for the
AgMnxOy/C catalyst was -0.51 mA.cm-2 at -0.22 V. In addition, the average particle size of AgMnxOy/C was
137.60 ± 12.19 nm, approximately 1.4 times larger than that of the AgVxOy/C catalyst.

Keywords: alloy catalysts, cathode electrodes, reducing sugar, and alkaline fuel cells

การประชุมวิชาการระดับชาติ วิทยาศาสตร์วิจัย ครงั้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-P-06 139

The Adsorption Capacity of NiAl-Layered Double Adsorbents on Removing Hexavalent
Chromium in Water

Mesa Na Nakorn1 Anamika Kaewnok1 Jakkapat Sermkaew1 Panpruek Pruekthikanee1 Kraisak Jantarakomet2 Panita
Sumanatrakul3 and Sonchai Intachai3,*
1 Paphayompittayakom school, SCiUS – Thaksin university, Phatthalung, 93210
2 Demonstration School of Khon Kaen University (Suksasart), Faculty of Education, Khon Kaen University, Khon Kaen, 40002
3 Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung, 93210
* Corresponding author email: [email protected]

Heavy metal is recognized as a serious harmful chemical on ecosystem and human, nevertheless there are
the usages as raw material for medical, agricultural, scientific and engineering applications and so on.
Chromium (Cr) has an atomic number of 24, and is a strongly oxidizing agent which affects respiratory,
carcinogenic, renal, hepatic, etc. In chemical nature, chromium has stably two oxidation states of trivalency
(+3) and hexavalency (+6), however in term of octet rule, hexavalent chromium is more hazardous and generally
formed in oxide anions, like chromate (CrO42 ) and dichromate (Cr2O72 ). In this work, the removal of
hexavalent chromium (Cr2O72 ) in water was investigated with capturing by NiAl-layered double adsorbents
due to their dominant presence of large surface area and positively charged surface. NiAl-layered double
hydroxide (NiAl-LDH) was prepared using co-precipitation method by mixing an aqueous solution of NiCl2 and
aqueous solution of AlCl3, the solution pH was adjusted by conc. ammonia solution until 9, then incubated at
80 C for 24 h. The preparation of NiAl-layered double oxide (NiAl-LDO) was conducted by calcining NiAl-
LDH at 400 C for 2 h. Two samples were characterized by XRD, FTIR, SEM, BET and DR/UV-VIS. The
determination of hexavalent chromium (Cr2O72 ) amounts was performed by UV-VIS. The adsorption capacity
of NiAl-LDO was better than that of NiAl-LDH arising from the change in the microstructure with the resulting
larger surface and active sites. The adsorption data showed best fitting to Langmuir isotherm model for NiAl-
LDH and Freundlich isotherm model for NiAl-LDO, and the adsorption mechanism followed pseudo-second
order kinetics.

Keywords: Chromium, NiAl-layered double hydroxide, NiAl-layered double oxide, Dichromate, Adsorption,

การประชมุ วชิ าการระดับชาติ วิทยาศาสตรว์ จิ ัย ครัง้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-P-07 140

Determination of leaching of Nitrate and Phosphate fertilizers from a flowerpot made from
a leaf sheaf of banana tree and natural rubber

Ratana Sananmuang 1,2,* Panita Meegaew1,2 and Wipharat Chuachuad Chaiyasith1,2
1 Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok, 65000
2 Center of Excellence for Petroleum, Petrochemical and Advanced Materials, Naresuan University University, Phitsanulok,
65000
* Corresponding author email: [email protected]

The objective of this work was to determine the leaching of a nitrate and phosphate fertilizers from a flowerpot
made from leaf sheaf of banana tree and natural rubber. The studied ratios of natural rubber and leaf sheaf of
banana tree were 100: 0, 75:25, 50:50 and 25:75. The biodegradation and water adsorption were also studied.
The 50:50 ratio of natural rubber to leaf sheaf of banana tree flowerpots were selected to add both of fertilizers
and studied a leaching. The amount of both fertilizers was measured by UV-VIS Spectrophotometry technique.
The results indicated that the 25:75 ratio of natural rubber to leaf sheaf of banana tree flowerpots had a highest
degradation, 5 2 . 1 9 % , at 3 0 days. There was a steady increasing in water adsorption as the time increase
(34.77% at 10 min to 101.96% at 42 h). The 50:50 ratio flowerpots could increase leaching the nitrate ions as
the time increase while that of the phosphate ions decreased as the time increases. Therefore, the 50:50 ratio
flowerpot could be selected for a practical use.
Keywords: leaf sheaf of banana tree, natural rubber, flowerpot, Nitrate fertilizer, Phosphate fertilizer

การประชมุ วิชาการระดับชาติ วทิ ยาศาสตรว์ ิจัย ครงั้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-P-08 141

Quantitative determination of phenolic content and antioxidant activity of Phulae Pineapple
waste’s masking powder

Jinda Sirta 1,*, Waleepan Rakittikul1 and Supawadee Keawpama1
1 Program of Chemistry, Faculty of Science and Technology, Chiang Rai Rajabhat University, Chiang rai, 57100
* Corresponding author email: [email protected]

This research aimed to determine the quantity of phenolics and antioxidant activity of phulae pineapple waste’s
powder, for the further development of the masking powder product containing phulae pineapple waste. The
sample preparation process of phulae pineapple peel was thoroughly rinsed with clean water, dried in the
shade, crushed and finally dried using two drying processes; oven-dried at 50 oC and freeze-dried. Two samples
from processes of drying were analyzed for the quantities of total phenolic content, antioxidant activities and
total vitamin C by UV-Vis Spectrophotometry. The sample of phulae pineapple waste’s powder by freeze-dried
process was found to have the higher phenolic content (0.470 g GAE/100 g of sample), antioxidant activity by
β-carotene bleaching method (57.0 %inhibition at 80.1 mg/mL of sample) and total vitamin C (1.89 mg/g of
sample) than oven-dried process. Also, the microorganisms content were tested using total plate count assay,
the sample of phulae pineapple waste’s powder by freeze-dried process was found that have no the
microorganisms content in this product which corresponded with Food and Drug Administration Standard 152-
2555.
Keywords: Phulae pineapple waste, Masking powder, Phenolic content, Antioxidant activity

การประชุมวชิ าการระดับชาติ วิทยาศาสตรว์ ิจัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-P-09 142

Effect of some organic dyes on the critical micelle concentration of sodium dodecyl
sulfate

Tanaporn Duangdech1, Tidatip Suwan1, Angkana Yama1, and Chakkresit Chindawong1,*
1 Department of Chemistry, School of Science, University of Phayao, Phayao, 56000
*Corresponding author email: [email protected]

In this research the effect of five organic dyes (acridine orange (AO), methylene blue (MB), congo red (CR),
methyl orange (MO) and methyl red (MR)) on the critical micelle concentration (CMC) of anionic surfactant
sodium dodecyl sulphate (SDS) were investigated by using uv-visible spectrophotometer and fluorescence
spectrophotometer. For uv-visible spectrometric measurement, the value of CMC determined from sigmoidal
curve is 5.04 mM by using AO dye probe in pH of 7 and 4.35, 5.40 and 5.24 mM by using MB dye probe in
pH of 3, 7 and 11, respectively. For fluorescence spectrometric measurement, the value of CMC determined
from the sigmoidal curve is 5.20, 6.84 and 6.43 mM by using AO dye probe in pH of 3, 7 and 11, respectively.
Using CR, MO, MR as dye probe, the value of CMC was not determined from the sigmoidal curve in both uv-
visible and fluorescence spectrometric measurements. The value of CMC is 8.23 mM as determined by a
conductometric method with a solution of SDS in the absence of dye. The findings of this research can be
applied for modelling study of dye-micelle interactions and potential applications for molecular biosensors.
Keywords: Micelle, Critical micelle concentration, Sodium dodecyl sulphate, Organic dyes

การประชมุ วชิ าการระดับชาติ วทิ ยาศาสตร์วจิ ัย ครัง้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-P-10 143

Reduction of accumulated nitrate in hydroponic vegetable using Bio-Slurry from fish
processing wastes

Niramol Juntarachat1,* Usa Onthong2
Faculty of Science, Thaksin University, 222 M. 2 Banpraw sub-district, Pa-payom district, Phattalung 93210
Corresponding author, email: [email protected]

This research focuses on the application of anaerobic digestion wastewater from fish processing wastes for
reducing nitrate accumulated in hydroponic vegetable. The effect of the ratio of chemical fertilizer and biogas
slurry on growth rate and nitrate contamination of morning glory was studied. Five ratio between chemical
fertilizer solution (consisting of solution A and solution B (1: 1)) and biogas slurry 100: 0 80: 20 60: 40 40: 60
and 0: 100 were prepared and used for hydroponic. The growth rate (stem height, leaf length, leaf width, and
leaf number) was measured every 5 days. Morning glory cloud rapidly grown at the ratio of 40: 60. The nitrate
content of the vegetable was determined by brucine calorimetric method. The experimental results showed that
the lowest amount of nitrate residue in morning glory was 1106 mg/kg of vegetables, which planted at the ratio
of solution, chemical fertilizer and wastewater from anaerobic fermentation system at 40: 60. Therefore, the
use of chemical fertilizer solution mixed with biogas slurry cloud be used for growing hydroponic vegetables
with a good growth rate as well as minimize nitrate residue in vegetable.
Keywords : Bio-slurry, Nitrate, Hydroponic vegetables, Bio-fertilizer

การประชุมวชิ าการระดับชาติ วิทยาศาสตรว์ จิ ัย ครัง้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-P-11 144

Encapsulation of Mulberry leaf extract in chitosan nanoparticle to develop eco-friendly
edible coating for fresh strawberry reservation

Somruthai Tunma*
Faculty of Education (Chemistry), Chiang Rai Rajabhat University, Chiang Rai, 57100
Corresponding author, email: [email protected]

In this research, Morus alba Mulberry leaf extract was encapsulated in chitosan nanoparticles by ionic gelation
method to develop coating for fresh strawberries. We aimed to study the effect of mulberry leaf extract loaded
chitosan nanoparticle coating on the shelf life of strawberry. The obtained nanoparticles exhibited a regular
distribution and spherical shape with the average size range of 87.23 to 139.5 nm as observed by using a laser
light scattering equipment and SEM technique. The results of FTIR studies was clearly indicated that the
nanochitosan gets effectively bonds with the molecules of secondary metabolites in Morus alba Mulberry leaf
extract. The FTIR spectra showed the present of peak at 1163.38 cm-1 which represented the interaction
between NH3+ of chitosan and PO43- of tripolyphosphate crosslinker and the peaks at 3402, 1078, 1023 and
699 cm-1 which indicated the presence of C-N, C-OH, -C-O-C- and N-H bonds of protein molecules of secondary
metabolites in Morus alba Mulberry leaf extract. The XRD pattern showed the change in crystallinity of chitosan
to the amorphous polymer region of chitosan nanoparticles. The effect of mulberry leaf extract loaded chitosan
nanoparticle coating on the shelf life of strawberry showed slower deterioration compared to uncoated
strawberry at 12 days while the uncoated strawberry started to decay at day 4. According to the result of
antimicrobial activity which was obtained using the disc diffusion method, only the 40 mg/ml mulberry leaf
extract loaded chitosan nanoparticle inhibited the growth of Bacillus subtillis with the mean inhibition zone
diameter of 7.870.20 mm. The cytotoxic effects of mulberry leaf extract loaded chitosan solution on the
viability of hGF cell lines were presented as the percentage of cell ability of which the sample at 1–10,000
g/mL concentration were 98.26±10.25% - 88.37±7.54%. Based on these results, encapsulation of mulberry
leaf extract in chitosan nanoparticle showed the higher effectiveness against of B. subtillis than the chitosan
nanoparticles and mulberry leaf extract without encapsulation. The edible coatings with mulberry leaf extract
loaded chitosan nanoparticles could be a promising strategy to improve the post-harvest quality of fresh fruits
and substitute other coated materials currently in use.
Keywords: encapsulation, chitosan nanoparticle, ionic gelation, mulberry leaf

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วจิ ัย ครง้ั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-P-12 145

Determination of fatty acids in alkyl ester obtained by transesterification via
heterogeneous catalyst from seed oil

Namfon Baothongkam, Chaturong Suparpprom and Anusorn Vorasingha*
Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok, 65000
*Corresponding author email: [email protected]

Determination of fatty acid in natural oil samples by using heterogeneous catalyst (CaO/Al2O3) was performed
in this study. Natural seeds oil (Palm kernel, Samrong, Sacha Inchi and Macadamia oils) has been obtained
by hot pressed extraction method. It is known that individual fatty acids can be identified by gas chromatography
(GC) because of their different retention times. The samples of natural seeds oil were esterified to bring them
into a vaporous phase, transforming the fatty acid from natural seeds oil into fatty acids alkyl esters. Oils were
changed into alkyl ester by solid catalyzed transesterification with various conditions including oil to alcohol
molar ratios (1:6, 1:9 and 1:12), temperatures (50, 60 and 70 oC), rates of stirring (300, 400 and 500 rpm), and
solid catalyst concentrations (3.0, 4.0 and 5.0 %). Oil to alcohol (methanol or ethanol) molar ratio of 1:12 with
5.0% solid catalyst concentration, 500 rpm mixing intensity under the reaction temperature of 70 oC showed
the best conditions to transform triglyceride to alkyl esters (FAAEs) which were further analyzed for fatty acid
compositions by GC technique. From the GC analysis, the amounts of fatty acids (caprylic acid, lauric acid,
myristic acid, palmitic acid, stearic acid and oleic acid) in natural oils were found. Therefore, the conversion of
oils to fatty acid alkyl esters by transesterification method can be used to analyze the amount of various fatty
acids in oil extracted from plant seeds. The volume of total oil content from natural seeds (Palm kernel,
Samrong, Sacha Inchi and Macadamia oils) were 60.79, 48.12, 55.36 and 76.84%, respectively.

Keywords: alkyl ester, glycerol, heterogeneous catalyst, transesterification

การประชุมวิชาการระดับชาติ วทิ ยาศาสตร์วจิ ัย ครง้ั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-P-13 146

Synthesis of piperine amide analogs as acetylcholinesterase inhibitors

Suwichada Jaipea1, Kornkanok Ingkaninan2, Waraporn Sutcharitruk1, Natthiya Saehlim1, and Rungnapha Saeeng1*
1Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Burapha University,
Sangsook, Chonburi 20131, Thailand.
2Faculty of Pharmaceutical Sciences, Naresuan University, Tha Pho Subdistrict Tha Pho, Mueang Phitsanulok District 65000,
Thailand.
*Corresponding author, email: [email protected]

Piperine, a major natural compound commonly found in black pepper, exhibited diverse biological activities
such as anti-oxidant, anti-inflammatory, anti-microbial including the activity of improving memory impairment.
In this work, piperine was isolated from black pepper (Piper nigrum L.) and converted to new piperine analogues
by chemical modification They were subsequently studied toward their inhibitory activity against acetylcholine
esterase (AChE) enzyme. Structure modification of natural piperine was carried out via a two-step. Esterification
of piperine to piperic acid followed by ammonolysis of using various amines led to a series of twelve new
piperine amide analogs in moderate to excellent yields. Among piperine amide analogues, (2E,4E)-5-
(benzo[d][1,3]dioxol-5-yl)-1-(4-methylpiperazin-1-yl)penta-2,4-dien-1-one (3b) show higher inhibitory activities
against AChE enzyme than piperine.
Keywords : piperine, black pepper, and esterification

การประชุมวชิ าการระดับชาติ วทิ ยาศาสตร์วจิ ัย คร้งั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-P-14 147

Synthesis and biological evaluation of alkynyl glycoside analogues as tyrosinase inhibitors

Natthiya Saehlim1, Anan Athipornchai1,2, Uthaiwan Sirion1,2 and Rungnapha Saeeng1,2,*
1 Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Burapha University,
Sangesook, ChonBuri, 20131
2 The Research Unit in Synthetic Compounds and Synthetic Analogues from Natural Product for Drug Discovery (RSND),
Burapha University, Chonburi, 20131
* Corresponding author email: [email protected]

A New series of alkynyl glycoside analogues were designed, synthesized and evaluated for their inhibitory
activities on mushroom tyrosinase. Both terminal and non-terminal alkynic moieties were incorporated to the
sugar ring at C-1 and C-6 positions through Fischer glycosylation and alkylation reactions. The inhibitory abilities
of alkynyl glycosides were in vitro investigated on mushroom tyrosinase for the catalysis of L-Tyrosine and L-
DOPA as substrates and compared with arbutin and kojic acid. Twenty-one compounds exhibited potent
activities with IC50 values ranging from 34.3 to 494 µM. In particular, compound 2b showed the most potent
inhibitory activity against L-DOPA with IC50 values of 34.3 µM higher than arbutin (IC50 13.3 mM) and kojic acid
(IC50 0.11 mM). The kinetic studies indicated that compound 2b was a non-competitive type inhibitor with a Ki
value of 21 µM and formed an irreversible receptor complex against mushroom tyrosinase. Molecular docking
showed a good fit in the pocket site of tyrosinase enzyme and had a binding energy of -7.80 kcal/mol. The
SARs results showed that the type of alkyne substituents on sugar played an important role in determining
their inhibitory activities.

Keywords: Alkynyl glycoside, Alkyne, melanin, sugar, Tyrosinase inhibitors

การประชมุ วชิ าการระดับชาติ วทิ ยาศาสตร์วิจัย ครั้งท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

CHEM-P-15 148

Design and synthesis of new 14-deoxy-11,12-didehydroandrographolide analogues

Patcharee Arsakhant 1,2, Sudarat Sombut1 Nittaya Boonmuen3 and Rungnapha Saeeng1,2,*
1 Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Burapha University,
Chonburi, 20131,
2 The Research Unit in Synthetic Compounds and Synthetic Analogues from Natural Product for Drug Discovery (RSND),
Burapha University, Chonburi 20131
3 Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
* Corresponding author email: [email protected]

14-Deoxy-11,12-didehydroandrographolide 1, a minor diterpenoid lactone from Andrographis paniculata, shows
some biological activities such as anti-influenza A, anti-inflammatory, anti-cardiovascular, anti-diabetic and anti-
cancer activities. In our present work, we designed and synthesized new 14- deoxy- 11,12-
didehydroandrographolide analogues by introducing benzoyl and cinnamoyl groups at C-19 position and acetyl
at C-3 of 1. Twenty analogues of 19-O-cinnamoyl and 19-benzoyl-3-O-acetyl-14-deoxy-11,12-
didehydroandrographolide analogues were obtained in moderate to good yield. All synthetic analogues were
further studied for anti-inflammatory activity.
Keywords: 14-deoxy-11,12-didehydroandrographolide, Andrographis paniculate, anti-inflammatory activity

การประชุมวิชาการระดับชาติ วิทยาศาสตรว์ จิ ัย ครงั้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-P-16 149

Surface treatment of hemp fiber and core on mechanical property of
poly(lactic acid) / hemp composite

Saori Watanuki and Supatra Pratumshat*
Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok, 65000
Corresponding author e-mail:[email protected]

This research studied the effect of surface treatment of hemp fiber and core by using sodium hydroxide and
silane coupling agent i.e; 3-(Trimethoxysilyl) propyl methacrylate (S1), (3-Aminopropyl) Trimethoxysilane (S2),
and Trimethoxymethylsilane ( S3) . Concentration of silane solution, time and temperature are varied. FT- IR
spectra show that hemp fiber and core treated by sodium hydroxide can remove lignin and hemicellulose. The
suitable condition for silane treatment is concentration 0.5 %v/v for 1 hour at room temperature. XRD results
show diffraction peaks of hemp core treated by sodium hydroxide and silane are at 16.7° and 22.5° but lower
crystallinity than untreated hemp core. S1 and S2 treated hemp core 5% by weight were mixed with poly(lactic
acid) ( PLA) by using twin screw extruder. Polymer compounds were compressed into sheet by using
compression molding machine. Tensile testing reveals tensile strength at break of both silane treated hemp
core/PLA composites are similar and slightly higher than sodium hydroxide treated hemp core/PLA composite.
Modulus of PLA is lower than other composites. Modulus of sodium hydroxide treated hemp core/ PLA
composite is similar to modulus of S2 treated/ PLA composite but slightly higher than that of S1 treated PLA
composite.
Keywords: chemical surface treatment, hemp fiber, hemp core, poly(lactic acid), silane coupling agent

การประชมุ วชิ าการระดับชาติ วิทยาศาสตร์วิจัย คร้งั ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

CHEM-P-17 150

Preparation and characterization of biodegradable nursery pots from thermoplastic starch
of pineapple starch and polymer

Tanawat Ketprasit, Jittra Bunrueang, and Supatra Pratumshat*
Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok, 65000
Corresponding author, email: [email protected]

This research studied the preparation and characterization of thermoplastic starch from pineapple starch.
Thermoplastic starch was blended with poly(lactic acid), PLA and fibrous and non-fibrous of pineapple leaf in
order to make good mechanical properties and biodegradable. Plasticizers used in this work are urea and
glycerol. It was found that the suitable weight ratio of pineapple starch: urea solution: glycerol was 12:80:8 at
90 °C for 20 minutes. XRD pattern of thermoplastic starch shows amorphous peak which is different from
pineapple starch that shows crystalline peak. Thermoplastic starch was blended with PLA and whole ground
pineapple leaf (WGL) (fibrous and non-fibrous material) at weight ratio 95:5 for 4 formula. All components were
mixed by twin screw extruder and then compressed by compression molding. Tensile testing reveals the tensile
strength of PLA:TPS:WGL decrease while the percentage of elongation at break increase compared with PLA.
The samples were buried in soil and weighted for biodegradability test. The polymer compounds were heated
in silicone mold into nursery pots at 180 °C 90 minutes.
Keywords: biodegradability test, plasticizer, pineapple starch, thermoplastic starch

การประชมุ วชิ าการระดับชาติ วิทยาศาสตรว์ จิ ัย ครัง้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วนั ที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวิทยาลัยนเรศวร

Poster Presentation

กลมุ่ ท่ี 3
สาขาวิชาคณติ ศาสตร์ คณิตศาสตร์ประยุกต์

สาขาวชิ าสถิติ

การประชุมวิชาการระดับชาติ วทิ ยาศาสตร์วิจยั ครง้ั ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันที่ 6-7 พฤษภาคม 2564 คณะวทิ ยาศาสตร์ มหาวทิ ยาลยั นเรศวร

MATH-P-01 151

Some numerical methods to estimate definite integral using Trapezoidal rule modifications

Apichat Neamvonk* and Kamonrat Peungyoo
Corresponding author, email: [email protected]

This research proposes some numerical methods to estimate a definite integral. The methods apply Trapezoidal
rule by replacing the straight lines that connecting two endpoints with other straight lines such as the tangent
lines, the secant lines, and linear regression line. The results show that the straight lines obtained by secant
line is closest to the exact solution and the relative error of regression line is less than that of Trapezoidal rule,
tangent line, and linear regression line.
Keywords: definite integral, Trapezoidal rule, linear regression

การประชมุ วิชาการระดับชาติ วิทยาศาสตรว์ จิ ัย ครัง้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร

MATH-P-02 152

Numerical Method for Definite Integral Estimation Via Regression Interpolation

Apichat Neamvonk* and Supattra Ngoenthong
Corresponding author, email: [email protected]

This research proposes some numerical methods to estimate a definite integral using linear and nonlinear
regression. These methods replace the integrand function which cannot be integrated to be a polynomial
function of several degrees. The results of the comparison showed that the approximate integral obtained by
nonlinear regression with a high degree polynomial function gives numerical results closest to the real value
and the relative error is less than the one with Trapezoidal rule, Simpson's rule1/3 and 3/8.
Keywords: definite integral, Trapezoidal rule, Simpson’s rule, nonlinear regression

การประชุมวชิ าการระดับชาติ วิทยาศาสตรว์ ิจัย ครงั้ ที่ 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร

MATH-P-03 153

Approximation of fixed points of two quasi nonexpansive mappings in CAT(0) spaces

Artitiya Intakrue 1,* and Watcharapong Anakkamatee 2
1 Department of Mathematics, Faculty of Science, Naresuan University, Phitsanulok, 65000
2 Department of Mathematics, Faculty of Science, Naresuan University, Phitsanulok, 65000
* Corresponding author email: [email protected]

In this work, we define a new iteration method for approximating the common fixed point of two quasi -
nonexpansive mappings in a complete CAT(0) space. We prove the strong convergence theorems with some
additional conditions which are the generalization of many previous results.
Keywords : CAT(0) space, common fixed point, quasi - nonexpansive mapping

การประชมุ วิชาการระดับชาติ วิทยาศาสตร์วจิ ัย ครงั้ ท่ี 12
THE 12TH NATIONAL SCIENCE RESEARCH CONFERENCE
วันท่ี 6-7 พฤษภาคม 2564 คณะวิทยาศาสตร์ มหาวิทยาลยั นเรศวร