NAPA Booklet1

O. 10-4 Developed Tapai Ubi Produced by Monocondiment Ragi: More Nutritive
and Healthy Food from Indonesia
Dr. Rindit Pambayun, is currently working as a Professor at The Department
of Agricultural Technology, Faculty of Agriculture Sriwijaya University, South Rindit Pambayun*, Triwardani Widowati, Budi Santoso, and Aldila Din Pangawikan
Sumatera, Indonesia. Dr. Rindit, graduated from Gadjah Mada University
(Undergraduate in 1983, Postgraduate in 1996, and Doctoral graduate in 2008). Agricultural Product Processing Department, College of Agriculture, Sriwijaya University,
In 1992, he took postgraduate program in Food Microbiology and Indonesia.
Biotechnology at Osaka University, Japan. Since 2010 he has been approved as
a professor in the field study of Food Science and Technology at the university. ABSTRACT
He is an active member of International Union of Food Science and Technology,
Indonesian Association on Microbiology and Molecular Biology. Now, he is the Tapai ubi (cassava) was fermented food made of cassava tuber by using ragi as
President of IAFT (Indonesian Association of Food Technologists). In 2017, he starter. Traditionally, ragi tapai was made of rice and certain spices such as garlic,
has assigned as the president of FIFSTA (The Federation Institutes of Food red onion, red chilly, and galangal. All were milled into pasta, flattened,
Science and Technology in ASEAN). He also received best Alumni Awards from incubated for two nights and then dried in the sun. The traditional ragi contained
The Rector of Gadjah Mada University, Best Inventor for Food Technology, molds, yeasts, and lactic acid bacteria. Improvement of tapai ubi quality began
South Sumatera Governor and Best Inventor in Food Research, Governor. Dr. with ragi development which focused on monocondiment formulation. The
Rindit also published many research articles in National and International monocondiment ragi was formulated only by using rice and garlic. The rice
Journals. should contain high amylose, whereas garlic should be free from red color and
dried peel. Rice was mixed with garlic with 2:1 ratio and then milled. These steps
were followed with dough making, ragi formation (round flat) (weight 3.2 g),
wind-drying, inoculation with 1.5 (w/w) per cent germ, fermentation at 28-32 C
for 2 nights and drying at max. 42 C. The microbes in the ragi were Aspergillus
rouxii, Rhizopus stolonifera, Mucor rouxii, Clamydomucor oryzae, Torulopsis
indica, Candida pelliculosa, Endomycopsis fibuligera, Hansenula anomala, and
Saccharomyces cerevisiae. Mostly of them were amylolytic fungy, which made
the tapai produced had more nutritive; more simple carbohydrate content, better
taste, and better quality. Furthermore, protein and thiamin increase 30 percent and
300 percent, respectively. Tapai was nutritive and healthy food.

Keywords:
tapai ubi, ragi tapai, fermented food

O. 10-5 Clitoria ternatea flower petals: anthocyanin-rich plant as functional
ingredient and the potential health benefits
Dr. Sirichai Adisakwattana, is a currently an Associate Professor at the
Department of Nutrition and Dietetics at the faculty of Allied Health Sciences, Sirichai Adisakwattana
Chulalongkorn University, Thailand. He is currently the Chairman of Master and
Doctoral program in Food and Nutrition (International program, Faculty of Allied Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn
Health Sciences, Chulalongkorn University. Dr. Sirichai, also the Deputy Dean University, Bangkok, Thailand
for Academic Affair, Chulalongkorn University. He was graduated from
Chulalongkorn University and later joined in the same University. His research ABSTRACT
interests are in the study of bioactive phytochemical compounds from edible
plants, fruits, and vegetables for prevention of diabetes and its complications. Dr. Clitoria ternatea L. is an edible flowering plant which belongs to the Fabaceae
Sirichai, served as a board and advisory member of the Nutrition Association of family. It is an important plant widely grown in the tropical and temperate regions
Thailand under the Patronage of Her Royal Highness Princess Maha Chakri of the world. The blue color of Clitoria ternatea flower containing anthocyanins
Sirindhorn. He has been recognized for his work on bioactive phytochemical is widely used as a natural source of food colorant for the preparation of various
compounds with the Young Research Award Ratchadaphiseksomphot delicacies. The published reports show that Clitoria ternatea flower extract
Endowment Fund Chulalongkorn University (2010), Ajinomoto Young possess anti -glucosidases and -amylase, anti-antioxidant, anti-glycation and
Researcher Award (2015), and Chula Research Scholar (2016), Thailand. He has hemolytic activities. In food uses, Clitoria ternatea flower lessens lipid
guided many graduate students and 10 years of teaching experience. He also peroxidation and increases antioxidant capacity in cooked meat and sponge cakes.
published numerous research articles in various International and national Bread and rice incorporated with Clitoria ternatea exhibits lower rate of starch
Journals. hydrolysis. Microencapsulation improves antioxidant capacity, pancreatic -
amylase inhibitory activity and bile acid binding of Clitoria ternatea flower. In
human study, Clitoria ternatea beverage decreases postprandial plasma glucose
response of sucrose and increase antioxidant capacity in healthy subjects. Taken
together, anthocyanin-rich Clitoria ternatea may be a functional ingredient for
health benefits.

Key words:
Clitoria ternatea flower, functional ingredient, antioxidant, health benefits

O. 11-1 Hydrogen Biology and Hydrogen Medicine: A New and Potential Area for
Physiology or Medicine
Dr. Jiankang Liu, is working as a Professor, at Center for Mitochondrial Biology
and Medicine and Center for Translational Medicine, The Key Laboratory of Jiankang Liu1,*
Biomedical Information Engineering of Ministry of Education, School of Life
Science and Technology and Frontier Institute of Science and Technology, Xi’an 1 1st Center for Mitochondrial Biology and Medicine and Center for Translational Medicine, The Key
Jiaotong University, China. Dr. Jiankang Liu, received his BS from Xi’an Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and
Jiaotong Unviersity in 1982 and PhD of Medical Science from Okayama Technology and Frontier Institute of Science and Technology, Xi’an Jiaotong University, Xi’an, China
University School of Medicine in 1994. He completed post-doc training in Dr.
Bruce Ames laboratory at University of California, Berkeley and worked as a ABSTRACT
faculty at University of California at Berkeley, Children Hospital Oakland
Research Institute, University of California at Irvine, University of Kentucky Hydrogen is the simplest and smallest molecule in nature. Because of the absence
College of Medicine, and Shanghai Institute for Nutritional Science, Chinese in air, it has not been thought of being a biological active or medical molecule.
Academy of Sciences. Currently, he is a Professor and the Dean of Xi’an Jiaotong Though the medical uses of high pressure hydrogen have been reported in 1888’s
University School of Life Science and Technology and an Adjunct Professor of (Diagnosis of gastro-intestinal perforation) and 1970’s (treatment of mouse
the School of Pharmacy, University of Southern California. Dr. Liu’s research squamous cell carcinoma), it has not been well appreciated due to its safety issue
interests include molecular and cellular mechanisms of aging, stress, and age- of explosion. However, Dr. Ohta at Japan published a historical paper in Nature
/stress-associated diseases with a focus on mitochondrial metabolism. He has Medicine in 2007 and reported that low concentration of hydrogen molecule (2-
published more than 180 SCI papers with H-index 61 and was elected as one of 4%) could protect brain ischemia-reperfusion damage in rats by selectively
the “2014, 2015, 2016 and 2017 The Most Cited Chinese Researchers” in the area scavenging toxic free radicals (hydroxyl radical and peroxinitrate). Since then,
of Biochemistry, Genetics and Molecular Biology by Elsevier. hydrogen molecule has been shown to have biological effects in animals and
plants and also have improving effects on nearly all kinds of diseases. In addition,
because of the high safety of low concentration of hydrogen molecule, a hydrogen
medical industry has formed rapidly, including hydrogen rich water/saline,
hydrogen gas treatment machine, hydrogen bath, hydrogen chamber etc. We
understand that the research on hydrogen molecule as a medical molecule is
greatly limited by the mysterious wide spectrum on various diseases and the
unsound acting mechanisms, nevertheless, it is time to have built a research area
as “Hydrogen Biology and Hydrogen Medicine”, a new area to study the
physiological and pathological effects of different hydrogen forms (hydrogen
molecule, positive and negative hydrogen ions and heavy hydrogen-deuterium
and tritium) and their potential therapy for various diseases as a
homeostasis/balance normalizer.

Keywords:
Hydrogen molecule, mitochondrial nutrient, redox homeostasis

O. 11-2 Rethinking the way of fighting bacterial resistance: synergy between plant-
derived products and antibiotics
Dr. Anca Miron is the Head of the Department of Pharmacognosy and Vice-
Dean of the Faculty of Pharmacy at Grigore T. Popa University of Medicine and Anca Miron*, Petruta Aelenei, Simon Vlad Luca,
Pharmacy Iasi, Romania. She received her PhD in Pharmaceutical Science at Adriana Trifan, Ana Clara Aprotosoaie
University of Medicine and Pharmacy Iasi, Romania. Her main areas of interest
are plant polyphenols and volatiles. A new direction in her research focuses on Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy
the identification of plant extractives/constituents exhibiting synergistic Iasi, Universitatii Str. 16, 700115, Iasi, Romania
interactions with antibiotics. Dr. Anca Miron has published over 40 peer-
reviewed articles in international journals (e.g. Archives of Toxicology, Critical ABSTRACT
Reviews in Food Science and Nutrition, Food and Chemical Toxicology, Natural
Product Research, Journal of Medicinal Food, Evidence-Based Complementary Despite huge progress in antibiotic development, bacterial resistance has raised
and Alternative Medicine etc). She was principal investigator in several national at alarming rates. Multidrug-resistant, extensively drug resistant and pandrug-
and international research grants and co-author of four national patents. Dr. Anca resistant bacteria still cause life-threatening infections. The search for novel
Miron received several national awards such as Servier Young Investigator antibacterial agents led to the identification of plant-derived products showing
Award (1998), Award for Pharmaceutical Research Activity (2002) and Award potent intrinsic antibacterial effects but also the capacity to enhance antibiotic
for Excellence in Pharmaceutical Research (2014). Dr. Anca Miron is guest editor activity or even reverse antibiotic resistance. The presentation reviews plant-
for International Journal of Molecular Sciences (special issue, 3rd International derived products reported to modulate antibiotic activity with a special emphasis
Symposium on Phytochemicals in Medicine and Food, Kunming 2018). on dietary plants; results of own researches are also included. In particular, we
investigated the antibacterial interactions between antibiotics and volatile or
phenolic extractives/constituents. Bacteriostatic and bactericidal synergistic
interactions were identified using the checkerboard and time-kill assays,
respectively. In several studies, we investigated essential oils from different
spices. For example, we found ajowan and coriander essential oils and their major
constituents (thymol and linalool, respectively) to act synergistically with
antibiotics against Gram-positive and Gram-negative human pathogenic bacteria.
We identified synergistic interactions between prenylated flavonoids (morusin
and kuwanon G from white mulberry, xanthohumol and 8-prenylnaringenin from
hops) and antibiotics but also antibiotic resistance reversal effects for morusin.
Studies investigating the mechanisms underlying antibacterial synergy are in
progress.

Keywords:
Essential oils, prenylated flavonoids, antibiotic resistance, synergy

O. 11-3 Diet, exercise, lifestyle and attention deficit/hyperactivity disorder (ADHD)

Dr. Klaus W. Lange, is a Professor at Biological, Clinical and Neuropsychology, Klaus W. Lange
University of Regensburg, Germany and a Director of Institute of Psychology, Institute of Psychology, University of Regensburg, 93040 Regensburg, Germany
University of Regensburg, Germany as well as a President of The International
Movement and Nutrition Society, Germany and President of The China Society ABSTRACT
for Culture and Science, Germany. Dr. Klaus, graduated from University of
Düsseldorf (faculty of Medicine) and University of Cologne (Doctorate) and Attention deficit/hyperactivity disorder (ADHD) is a common diagnosis in childhood and
worked as a Lecturer in University of Düsseldorf for a while and become adolescence and may persist into adulthood. The long-term pharmacotherapy of ADHD has no
professor and worked at University of Freiburg and University of Regensburg. proven efficacy and can be associated with adverse effects. This highlights the need to find
His research interest includes health effects of physical activity, sport, diet and alternative treatment approaches. Recent findings suggest that healthy lifestyle habits could be
lifestyle; Developmental clinical and experimental neuropsychology; Interaction helpful in the management of ADHD. While many studies have investigated medication effects
of biological and social factors in mental health; Prevention and public health in in ADHD, only a small fraction have assessed other forms of management. Growing evidence
child development and aging; Coaching of public health experts in academia and suggests a significant relationship between quality of diet and mental health. While there is
practice. He is an Editor-in-Chief for Movement and Nutrition in Health and currently no evidence supporting a role of single nutrients in ADHD, the relationship between
Disease, Germany and Scientific Editor, Food Science and Human Wellness, dietary patterns and ADHD may be of greater importance. Physical exercise has also been
China. He published more than 200 research articles in peer-reviewed suggested as treatment for ADHD. It may be well worth initiating well-designed studies
international scientific journals and 60 book chapters as well. He also presented investigating the effects of exercise on ADHD, especially since physical activity will have
his research data in several international conferences. additional health benefits. Certain lifestyle behaviors may provide effective interventions,
either together with or in the place of commonly used ADHD medications.

Keywords:
Attention deficit/hyperactivity disorder (ADHD), alternative treatment, mental health, exercise,

References:
Lange KW (2018). Lifestyle and attention deficit/hyperactivity disorder. Mov Nutr Health Dis
2: 22–30. DOI: 10.5283/mnhd.10

Lange KW (2018). Diet, exercise, and mental health. Mov Nutr Health Dis 2. DOI:
10.5283/mnhd.12

O. 11-4 Avian models development by CRISPR/Cas9-mediated genome
editing Technology
Dr. Jae Yong Han, is a Professor at Dept. of Agricultural Biotechnology,
College of Agriculture and Life Sciences, Seoul National University, Seoul, Jae Yong Han1,*, Hong Jo Lee1
South Korea. He is also a Distinguished Professor at Shinshu University, Japan.
Dr. Jae Yong Han received his bachelor and master degrees in Seoul National 1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life
University, Korea. Since his completion of Ph.D. degree in University of Sciences, Seoul National University, Seoul, 08826, South Korea
Minnesota in 1991, he appointed as a professor of Department of Agricultural
Biotechnology, Seoul National University. His academic specialties are avian ABSTRACT
transgenesis, genome editing and germ cell biology. He has made outstanding
achievements in his researches on production of avian germline chimera by germ Model animals have been used to study biological mechanisms in various
cell manipulation and genome editing. Specifically, his laboratory developed in organisms for a long time. Particularly, avian species have been recognized as
vitro long-term culture system of chicken primordial germ cells (PGCs) for ideal models due to their distinctive developmental characteristics. More
subsequent genome modulation of the PGCs. More recently, he has expanded to specifically, in chicken, diverse model animals have been developed by
his research to early embryo development of avian species for indepth programmable genome editing tools such as clustered regularly interspaced short
understanding of germ cells as well as embryo itself. Up to date, he has published palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) to be applied
more than 200 research articles in the internationally prominent journals. Based to basic science as well as applied science. In these regards, we developed a novel
on his achievements, he was awarded 2012 World’s Poultry Science Association genome editing strategy by applying CRISPR/Cas9 to chicken primordial germ
Award (Research), and now he runs the project “Center for Avian Germ Cell cells (PGCs) which are precursors of mature germ cells for avian model
Modulation and Cloning” awarded by National Research Foundation of Korea. development. Using the simple and efficient knock-in method, we developed
diverse chicken models, including novel germ cell tracing model by tagged a
germ cell-specific gene and sexing chicken model by targeted gene insertion to
chicken sex chromosome. Our results suggest that the innovative genome editing
strategy could contribute to develop diverse avian models more easily and
efficiently and to facilitate the applications of avian models.

Keywords:
Avian species, chicken, model animal, CRISPR/Cas9, genome editing.

O. 11-5 Mechanism of Skeletal Muscle Disuse Atrophy: Implication in Sarcopenia

Dr. Li Li Ji, is Professor and Director of the Laboratory of Physiological Hygiene Li Li Ji,
and Exercise in the School of Kinesiology since 2011. Dr. Ji graduated from East
China Normal University, received his MS and PhD at the University of The Laboratory of Physiological Hygiene and Exercise Science,
Wisconsin-Madison in 1982 and 1985, respectively, specializing in exercise University of Minnesota Twin Cities, USA
physiology. During 1985-1987 he was a postdoctoral fellow at the Institute of
Enzyme Research of UW-Madison. He served as Assistant and Associate ABSTRACT
Professor at University of Illinois at Urbana-Champaign during 1987-1993 and
returned to UW-Madison as Associate Professor in 1994. Dr. Ji was Professor of Skeletal muscle contraction is required for the intracellular signaling to maintain
Exercise Physiology in the Biodynamics Laboratory from 1997-2011. Dr. Ji’s muscle mass and function. Muscle disuse activates degradation pathways and
research expertise is cellular and molecular exercise physiology, especially the imposes oxidative stress resulting in myocyte atrophy and functional
role of free radicals and antioxidants in muscle function, adaptation to exercise, deterioration. Aging also deteriorates mitochondrial morphological and
pathogenesis and prevention, cell signaling and aging. His recent research is functional integrity, which has a significant impact on the development of
focused on mitochondrial quality control and its relationship with exercise sarcopenia. Mitochondrial homeostasis (mitostasis) is regulated by several
adaptation, muscle atrophy and aging. Dr. Ji has published over 150 peer- distinct but interrelated pathways including mitochondrial biogenesis,
reviewed articles and 23 book chapters, and given over 160 invited international mitochondrial dynamics (fusion and fission), and mitophagy. A downregulation
and domestic lectures. He has served on multiple editorial boards and study of PGC-1α and activation of FoxO with muscle immobilization appear to underlie
sections of federal agencies and private foundations. He is a member of the the disintegration of the crosstalk between intracellular pathways leading to
National Academy of Kinesiology since 2006 and a Fellow of the American decreased biogenesis, enhanced fission and mitophagy, increased oxidative stress
College of Sports Medicine since 1990. and inflammatory responses. Aging mimics muscle disuse atrophy by a decline
of PGC-1α level and increased mitophagy, as well as initiating apoptosis. The
lack of contractile activity in disused muscle and aged muscle makes in vivo
PGC-1α transfection and dietary supplementation of select bioactive agents
promising strategy to improve muscle mitochondrial function. While these
physiological, genetic and nutritional interventions may not entirely prevent
muscle mass and protein loss, they can ameliorate mitochondrial quality and thus
improves muscle overall health and function.

Keywords:
Muscle disuse, aging, sarcopenia, PGC-1α, FoxO.

O. 11-6 The improvement of eyes vision by using the combination of lutein,
blueberry and wolfberry-- a clinical trial
Dr. Chin-Kun Wang, is a Distinguished Professor in Chung Shan Medical University,
President of International Society for Nutraceuticals and Functional Foods (ISNFF), Chin-Kun Wang
Fellow of International Academy of Food Science & Technology (IFOST), Honorary
President of Nutrition Society of Taiwan (NST), Director for International Life Former President, Chung Shan Medical University (CSMU)
Science Institute, Taiwan. He got his PhD from National Taiwan University, Taiwan Honorary President, Nutritional Society of Taiwan
and working at Chung Shan Medical University, since 1993. In 1996, he promoted as
a Full Professor, and then took the positions of Chair, Dean, Vice-President and ABSTRACT
President in Chung Shan Medical University. His research work is focused on human
clinical trials and human metabolism of medicine, nutritional supplement, Eyes health problem is usually induced under high stress environment, including
nutraceuticals, herbs, and functional foods. He got the National Award of Biomedicine long term use of cell phone, computer and pad. Lutein, blueberry and wolfberry
for his great contribution to the medical education in 2008. He was also honored as are well known as their natural antioxidant activity and eyes protection. This
2012-18 Who’s who in the world, Who’s who in Asia, and 2009-2010, 2011-18 Who’s study is to evaluate the improvement of eyes vision by using the combination of
who in Medicine and Healthcare. He also got the awards of outstanding research from lutein, blueberry and wolfberry (LBW). Fifty-six subjects with myopia or eyes
several societies from 2009 to 2018. Dr. Chin-Kun Wang was the former president of fatigue or over eyes use were included. One month before experiment was run in
Nutrition Society of Taiwan (from 2009 to 2012). For food safety and nutrition, he period. Totally intervention period was 5 months. One month after stop LBW
promoted the legislation for school sanitary law and national nutrition law. He believes drink was follow up period. Each subject drank one bottle of LBW drink (60 ml)
that scientific evidence is the best support for food safety and world nutrition problem. every day for continuous five months. Blood samples were collected individually
In the future, he tries his best to work together with the scientists around the world by at 0, 3 and 5 months. Eyes' functions were evaluated every month. After five
network. months of intervention, eyes comfort, BCVA, stereoscopic vision, amplitude
accommodation, intraocular pressure, contrast sensitivity, MPOD, blood lutein,
antioxidant status and phenolic levels were all greatly improved. This indicates
that LBW drink could protect eyes, improve vision and promote antioxidation.

Keywords:
lutein, blueberry, wolfberry, eyes over use, vision improvement

O. 12-1 Caloric Restriction: mechanisms and benefits for Aging and Cancer
prevention
Dr. Ciro Isidoro, is a full professor at Department of Health Sciences; Università del Piemonte
Orientale, Novara, Italy. He did his doctorate (Medicine and Surgery) at the Università del Alessandra Ferraresi1, Chiara Vidoni1, Danny Dhanasekaran2, Ciro Isidoro1,2*
Piemonte Orientale, Italy and worked as assistant researcher at Institut fuer Pathobiochemie,
Germany and post-doc at Università di Torino, Italy. Later promoted as Assistant Professor 1Laboratory of Molecular Pathology and Nanobioimaging, Department of Health Sciences,
(General Pathology), University of Turin, Italy and become associate professor at Università University of Piemonte Orientale, Novara 28100, Italy
del Piemonte Orientale, Novara, Italy. Currently, he is also a Visiting Professor, Department
of Cell Biology, Oklahoma University Health Science Center, USA. His field of research 2Stephenson Cancer Center, The University of Oklahoma Health Sciences Center, US
interest are cancer biology- Autophagy in Cancer and in Neurodegeneration. Epigenetic
regulation of Autophagy and cell death. Anti-aging and anti-cancer Nutraceuticals. ABSTRACT
Complementary medicine. Organelle biogenesis, vesicular traffic and diseases.
Nanotheranostics (‘in cellulo’ imaging). He also published more than 140 international and Cell and tissue homeostasis can be maintained through the elimination and
national peer reviewed journals. Dr. Ciro Isidoro is a Co-Editor in Chief of J Traditional and replacement of unrepaired damaged structures/cells/molecules accomplished by
Complementary Medicine and Associate Editor of Autophagy, Molecular Carcinogenesis, the Autophagy-lysosomal pathway, a process that contributes to cell homeostasis
BMC Cancer, Genes and Cancer, J. Molecular Signaling, Current Biomarkers, Frontiers in by promoting the lysosomal degradation of damaged and redundant self-
Endocrinology and Ageing, Am J Cancer Biol etc. He had been awarded and honoured by constituents. Nutrient starvation has been shown potential benefits in prolonging
Professor Honoris Causa Faculty of Medicine and Pharmacy, Université de la Franche-Com the lifespan, while preventing age-related diseases, including cancer. As in cancer,
té of Besançon, France and Integrative Cancer Research Center of the Georgia aging is associated with accumulation of toxic substances due to failure in the
Institute of Technology, Georgia Tech University, Georgia, USA as well as executive Vice- removal and replacement of damaged structures/cells. The effects of starvation
President of the International Association of Traditional and Complementary Medicine. and of caloric restriction are mediated, among others, by autophagy. In fact,
Autophagy has been shown to counteract the aging-related effects in several
cellular models. Autophagy also prevents carcinogenesis. The efficiency of the
autophagy process declines with aging, which may explain the increased
occurrence of cancer during aging. Nutraceuticals mimicking a caloric/protein
restriction condition can substitute for aminoacid-protein poor diet to elicit anti-
aging and anti-cancer benefits. We show that caloric restriction mimetics, such
as Resveratrol, Halofuginone and Metformin, are potent inducers of autophagy
(mechanisms at cellular and molecular level).

Keywords:
Aging, Cancer, Autophagy, Caloric Restriction.

O. 12-2 New insights into old herbs - two case examples of biological activity of
TCM herbs grown in Europe
Dr. Adam Matkowski is currently a Professor and Head of Department of
Pharmaceutical Biology, Botany and Biotechnology, Wroclaw Medical Dorota Woźniak1, Marta Szandruk2, , Beata Nowak2, Izabela Nawrot-Hadzik1, Sylwester
University, Poland. Dr. Adam, graduated (Masters) from Jagiellonian University Ślusarczyk1, Jakub Hadzik3, Adam Matkowski1,4*
in Poland with major in Molecular Biology (specialized in Plant Biochemistry)
and received his PhD in Plant Biology at University of Wroclaw, Poland. During 1 Dept. Pharmacology, Wroclaw Medical University, Poland; 2 Dept. Pharmaceutical Biology,
the graduate research he was suing plant tissue culture methods to study Wroclaw Medical University, Poland; 3 Dept. Dental Surgery, Wroclaw Medical University,
developmental processes and the hormonal regulation of them. Thereafter, he has Poland, 4 Botanical Garden of Medicinal Plants, Wroclaw Medical University, Poland
been involved in studying plant specialized metabolism, focusing on the
medicinal plants which produce and accumulate an enormous diversity of ABSTRACT
metabolites, many of which are useful as natural medicines. The ultimate goal if
his research would be to elucidate reasons and mechanisms of this diversity, not Despite the ancient use and huge amount of data on pharmacological and clinical
only from the utilitarian viewpoint but also to help us understand how plants properties of the Traditional Chinese Herbs, there are still many undiscovered
evolved as efficient chemical factories to cope with the constantly changing phytochemicals and potentially novel directions of their activity. Some of these
environmental challenges. He has more than 50 peer reviewed full papers in JCR TCM herbs have only recently attracted more attention that lead to detailed
indexed journals and 100+ conference presentations (including several invited description of their chemical composition and added new data to their bioactivity.
and plenary lectures internationally and domestically) as well as several book Also, TCM herbs have become popular and adapted by non-Asian phytotherapy
chapters on bioactive plant metabolites (in Polish and English). and are listed for example in European Pharmacopoeia. Here, we will present two
examples of pharmacopoeial herbs growing in Europe which were studied for
various activities as well as phytochemically profiled. Belamcandae chinensis
rhizoma constituents such as xanthones, isoflavones, stilbenoids were not only
antioxidant, antimutagenic, and cholinesterase inhibitors in vitro, but were also
efficient against colon inflammation and osteoporosis in animal models. Polygoni
cuspidati rhizoma from invasive wild populations in Poland, contained high
amounts of stilbenes and anthraquionones and beside being strong antioxidants
and anti-inflammatory agents, they also inhibited growth of periodontitis -
associated microbes.

Keywords:
Belamcandae rhizoma, mangiferin, Polygoni cuspidati rhizoma, polyphenols.

O. 12-3 Health Benefits of Romanian folk preparations as modern life style
changers
Dr. ELVIRA GILLE is Head of NIRDBS/”Stejarul” Biological Research Centre,
Piatra Neamt, Associate professor at the ““Alexandru Ioan Cuza” University of Elvira Gille1,2, Ruxandra Cretu1, Elisabeta Sidoroff3, Radu Necula1,2, Camelia Paula
Iasi, Faculty of Chemistry (2017-2020), ROMANIA. Her main research interest Stefanache1, Monica Hancianu4, Oana Cioanca4
is focused on study of medicinal and aromatic plants (MAPs) from culture
(ecological and conventional) and wild flora, morpho-chemical evaluation of 1 NIRDBS Bucharest / "Stejarul" Biological Research Centre Piatra Neamt, Alexandrul cel
some vegetal natural population, bioproductivity, elaboration of vegetal extracts Bun 6, 610004, Piatra Neamt, Romania; 2 Faculty of Chemistry, “Alexandru Ioan Cuza”
and food supplements, vegetal cytogenetics, pest control with different vegetal University of Iasi, Bulevardul Carol I no 11, 700506 Iasi, Romania; 3 National Institude of
extracts, capitalization of vegetal wastes (from the processing of vines, vegetables R&D for Biological Sciences, Splaiul Independenței 296, 060031, Bucharest Romania; 4
and fruits, medicinal and aromatic plants) to obtain secondary products usable in Faculty of Pharmacy,“Grigore T. Popa” University of Medicine and Pharmacy, Universitatii
food supplements and cosmetic industries (collaboration with SMEs). Dr. Elvira
Gille was coordinator/partner in numerous national and international projects and 16, 700115, Iasi, Romania
also, as specialist in over 30 projects; she has 3 national patent applications and
two national patents; she published over 260 scientific papers in national and ABSTRACT
international journals (e.g. Planta Medica, Phytochemistry Reviews, Romanian
Biotechnological Letters etc.). In the period 2004-2006 she obtained prizes for Old traditional remedies are investigated today for their pharmacologic properties
complex projects achieved with private partners (prizes I, II). Also, she was main and toxicologic implications just as Romania follows new regulations in terms of
organizer of 2 international scientific meetings (PSE Conference nutraceutics. This is why in our research we started from well-known medicinal
“Phytochemicals in Medicine and Pharmacognosy” in 2014 and MAPPPS 2016, plants that have a long history of use in Eastern Europe and especially in our
Piatra Neamt, Romania) under Phytochemical Society of Europe. country. A simple search of different databases in Romanian language revealed
that in the last two years there were about around 168 registered companies that
are involved in food supplement distribution and formulation, but the number of
domestic manufacturers is significantly lower than that of the distributors.
Therefore, on the Romanian food supplement market there are both native and
exotic plants indicated for prevention and treatment of different ailments.
Moreover, some plant extracts are included in natural cosmetics indicated for
complementary applications along with the oral administration of food
supplements to increase the efficacy of the treatment. The most common example
is Heracleum sphondylium (Apiaceae), for which over 27000 results were given
for its indications and Romanian distributors, but only 8 major domestic
registered manufacturers provide standardized products. Nevertheless, our
extensive research includes several medicinal plants for which we also
investigated their activities and can confirm positive health benefits.

Keywords

Romanian folk preparations, medicinal plants, food supplements.

O. 12-4 Protein Nutritional Value and Sensory Characteristic of “Quick Tempe”
Comparing to the Conventional Tempe
Dr. Christofora Hanny Wijaya, is the Head of Food Chemistry Division at the
Department of Food Science and Technology, Bogor Agricultural University, Claudia E. Ginting, C. Hanny Wijaya* and Sutrisno Koswara
Indonesia. She obtained her Ph.D. from Hokkaido University in Japan in 1990.
Visiting lecturer and external examiner at Indonesian and overseas Universities Department of Food Science and Technology, Bogor Agricultural University (IPB)
i.e. National University of Singapore, Warsaw University of Life Science, Bogor, PO Box 220, Indonesia 16002
Hokkaido University (HU), Wisconsin University. Her major focus on finding
novel Functional foods/Nutraceutical from Traditional food and to improve the ABSTRACT
activity of various traditional food using food technology. Receiving several
national competitive and international joint research grants, she has scholarly “Quick Tempe” is a tempe made through chemical acidification by using Glucono
contributed to over 100 journal publications, 8 books, 3 review papers and several Delta Lactone (GDL), to reduce the water utilization and soaking time. This
chapter books including in Food Chemistry handbook published by Springer. She research is aimed to determine the quality of quick tempe, particularly the protein
has granted 10 approved patents. Now, she is the president of Indonesian nutritional value and sensory characteristic comparing to tempe made by
Association of Flavor and Fragrance, the chairperson of Indonesian Society of spontaneous acidification. The in vitro and in vitro nutritional assessment also the
Functional Foods and Nutraceuticals, also appointed as HU Ambassador. Some sensory evaluation using QDA and hedonic tests have been conducted. The
of received awards i.e. the JSPS Bridging 2018, Erasmus plus2017. She is an protein quality of quick tempe was not significantly different from the
active member of Indonesian Association of Food Technologists, Member of conventional tempe, and both were better than casein. The different of soaking
Association of Microbiology Indonesia and President of Association of Flavor process did not impact the nutritional value of both tempes. The acceptance of
and Fragrance Indonesia (AFFI), Hokkaido University Alumni Association and overall sensory attributes of quick tempe was not significantly different
Indonesian Association of Functional Foods and Nutraceuticals. (moderately liked) from the conventional one. However, the texture of
conventional tempe was more liked by the panelists. The raw quick tempe showed
more intense in rancidity and sour odor, however, more juicy and lower intensity
of unpleasant odor than the conventional tempe. The Fried quick tempe had
higher intensity of sour of odor, however, it was more juicy, solid, and lower
intensity of rancid, unpleasant odor, and bitter taste comparing to the
conventional tempe. The quick tempe might become one of environmental
friendly tempe processing.

Keywords:
Glucono Delta Lactone (GDL), nutritional value, quick tempe

O. 12-5 Pharmacological usefulness of functional foods and their beneficial and
harmful interaction with drugs
Dr. Shizuo Yamada, is a Director (Professor) of Center for Pharma-Food
Research (CPFR), Graduate School of Pharmaceutical Sciences, University of Shizuo Yamada*
Shizuoka, Japan. Dr. Yamada did his B.Sc. (1971), M.Sc. (1973), Ph.D. (1976)
in Pharmacology, Shizuoka College of Pharmacy. Pre-doctoral research associate Center for Pharma-Food Research (CPFR), Graduate School of Pharmaceutical Sciences,
at University of Nottingham and University of London, England (1975). Research University of Shizuoka, Japan.
Fellow, at Shizuoka College of Pharmacy (1976-1978). Postdoctoral Research
Associate, at University of Arizona, Tucson, USA. (1978-1980). Assistant ABSTRACT
Professor, at Shizuoka College of Pharmacy (1980-1988). Visiting Research
Fellow, at University of Arizona (1986). Assistant Professor, at University of Currently, the popularity of dietary supplements containing botanical products
Shizuoka (1988-1991). Associate Professor, at University of Shizuoka (1991- and foods is growing at a remarkable speed, in terms of the promotion of health,
2004). Visiting Professor at University of California (Los Angeles) (1994). prevention and therapy of diseases. We have investigated efficacy of some plant
Professor and Chairman, School of Pharmaceutical Sciences, University of extracts in the alleviation of lower urinary tract symptoms (LUTS). Peucedanum
Shizuoka (2004-present). Vice-president at University of Shizuoka (2011-2013). Japonicum (PJ) is one of umbelliferae plants, inhabited at southern parts of Japan,
Assistant President Adviser (2013-2014) and finally become Director (Professor) and its pharmacologically active constituent (isosamidin) exerted a concentration
at University of Shizuoka. Integrated study on pharmacokinetics, drug-receptor dependent inhibition of agonists-stimulated contraction of isolated strips of
binding and pharmacodynamics for actualization of drug discover and optimum bladder and prostate. Furthermore, PJ extract improved significantly urodynamic
drug therapy (currently, focusing therapeutic drugs for urinary dysfunction). symptoms in hyperactive rat bladders by decreasing the micturition frequency.
Published about 350 original article in peer-reviewed international journals) with Repeated oral administration of PJ extract improved significantly urodynamic
51 book chapters and 53 reviews. parameters in female patients with LUTS. Regarding pharma-food interaction
with medicine, fruit juices have been reported to inhibit organic anion-
transporting polypeptides, which are membrane transport proteins facilitating the
uptake of substrate drugs. In addition, green tea may be a contributing factor to
cause clinical pharma-food interactions. We have shown that the ingestion of
green tea in human affects significantly plasma concentrations of simvastatin and
nadolol, suggesting that more attention should be paid to green tea consumption
during pharmacotherapy.

Keywords:
plant extracts, lower urinary tract symptoms, pharma-food interaction, green tea

O. 12-6 Bioproduction, Synthesis Mechanism, and Cryoprotective Effects of Poly-
Gamma-Glutamic Acid
Dr. Bo Jiang is a Professor in State Key Laboratory on Food Science
and Technology, director of International Office, Jiangnan Bo Jiang
University, China. He received his BS in Chemistry at Nanjing State Key Laboratory on Food Science and Technology, Jiangnan University, China
University in 1982, MS and Ph. D. in Food Science at Wuxi Institute
of Light Industry (Currently, Jiangnan University) in 1989 and 1993, ABSTRACT
respectively. His main interests are in functional ingredients,
nutritional supplements and enzymology. The current research areas Cleaner label, enhanced health benefit, and cost reduction are among the key
include enzyme construction, bio-production of functional sugars, driving forces for the food industry. In addition to new food sensing and
oligosaccharides, modification of carbohydrates and amino acids processing technologies, the explorations of naturally occurring materials are
with relevant enzymes. He also published in several International and essential to food innovations. As a naturally occurring biopolymer, poly-γ-
National Journals. He is an Institute of Food Technology (IFT) fellow and an glutamic acid (γ-PGA) contains repeating units of glutamic acid. With its
active professional member, International Advisory Board member of characteristics of being biodegradable, non-toxic, and non-immunogenic, γ-PGA
International Society for Nutraceuticals and Functional Foods has broad applications in agricultural, food, cosmetics, medical, and other areas.
(ISNFF), board member of Chinese Institute of Food Science and This presentation will review γ-PGA including its biosynthesis and production,
Technology, receiving editor in journal Food Bioscience, and and thereafter elaborate its use for cryopreservation, a novel application of γ-PGA
executive editor of Journal of the Science of Food and Agriculture. with potential impact on the food industry.

Keywords:
Food Sensing, Food innovation, biopolymer, poly-γ-glutamic acid, Food Industry