NAPA Booklet1

O. 1-1 Modulation of tumor microenvironment by bioactive natural products

Dr. Young-Joon Surh, currently serves as Director (Professor) of Tumor Do-Hee Kim1, Jin-Young Suh1, Jisun Oh1, Hyo-Jin Yoon1, Jeong-Hoon Jang1, Sin-Aye Park1,
Microenvironment Global Core Research Center, Seoul National University Hye-Kyung Na2, Young-Joon Surh1,*
(SNU). He graduated from College of Pharmacy, SNU with Bachelor’s and
Master’s degrees. Dr. Surh earned a PhD degree at the University of Wisconsin- 1Tumor Microenvironment Global Core Research Center, College of Pharmacy, Seoul
Madison and had postdoctoral training at the Massachusetts Institute of National University, Seoul 08826, South Korea 2Department of Food Science and
Technology (MIT). In 1992, he was appointed as a tenure-track Assistant
Professor at Yale University School of Medicine. Since relocating to SNU in Biotechnology, College of Knowledge-Based Services Engineering, Sungshin Women’s
1996, Dr. Surh has been investigating the molecular mechanisms of cancer University, Seoul 01133, South Korea
chemoprevention with anti-inflammatory and antioxidative natural products, with
focus on intracellular signaling molecules as prime targets. He is currently ABSTRACT
Associate Editor of Molecular Carcinogenesis, Free Radical Research, Frontiers
in Nutrition, and Editorial Board member of Molecular & Cellular Biology, The tumor microenvironment provides a niche in which cancer cells and their
International Journal of Cancer etc. Dr. Surh has published more than 300 papers surrounding stromal cells reside and in which their interactions occur. The cross
in peer-reviewed international journals and about 70 invited editorials, reviews talk between cancer and stromal cells in the tumor microenvironment promotes
and book chapters with more than 18,000 citations. Dr. Surh received numerous many biological processes to support cancer cell growth, invasion, angiogenesis,
awards including Elizabeth C. Miller and James A. Miller Distinguished Scholar and metastasis. Recently, not only cancer cells but also multiple types of
Award (2011), McCormic Science Institute Award (2009) etc. Dr. Surh has surrounding stromal cells, including endothelial cells, immune cells, and
recently been elected as President of Korean Society of Molecular and Cellular fibroblasts in the tumor microenvironment, have been recognized to be attractive
Biology (KSMCB), the largest academic society in the field of biomedical targets for reducing resistance to anticancer therapy and tumor recurrence. Many
sciences in Korea. natural products present in fruits, vegetables, herbs, spices, and some marine
organisms have been reported to inhibit, delay, or reverse multistage
carcinogenesis and to inhibit the proliferation of cancerous cells and the self-
renewal capacity of preexisting cancer stem-like cells. Some of these naturally
occurring chemopreventive and anticarcinogenic substances can modulate the
signal transduction involved in maintaining the activities/functions of stromal
cells and their interactions with cancer cells within the tumor microenvironment.

Keywords:

Bioactive natural producs, Cancer-associated fibroblasts, Cancer Stem cells,
Tumor microenvironment

O. 1-2 Exploring novel cancer-related microRNAs and their diagnostic and
therapeutic potentials in Personalized Cancer Medicine (PCM)
Dr. Johji Inazawa, is currently working as Professor and Director of
Bioresource Research Center, Department of Molecular Cytogenetics, Medical Johji Inazawa
Research Institute (MRI), Tokyo Medical and Dental University (TMDU),
Tokyo, Japan. He graduated (medicine and doctorate) from Kyoto Prefectural Bioresorce Research Center, Tokyo Medical and Dental University (TMDU)
University of Medicine, Japan. Later he worked as a resident and lecturer at Department of Molecular Cytogenetics, Medical Research Institute (MRI), TMDU,
Kyoto Prefectural University of Medicine, Japan. Then, Joined as Associate
Professor in The University of Tokyo, Japan. Finally moved to Tokyo Medical Address: 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
and Dental University and joined as a professor. His speciality of research is
Cancer Genomics and Epigenomics, Medical Genetics. Dr. Johji Inazawa, has ABSTRACT
been awarded as a Young Investigator's Award of the Japanese Society of
Hematology, Young Investigator's Award of Kyoto Prefectural University of MicroRNAs (miRs) are endogenous small non-coding RNAs that regulate gene expression by
Medicine, JCA-Mauvernay Award, from Japan Cancer Association as well as interfering with translation and/or stability of target transcripts. In order to explore novel TS-
received an Honorary Diploma from The Bulgarian National Academy of miRs for the development of miR-based cancer therapy, we conducted function-based
Medicine. He is a Associate editor for the Journals Cancer Science and Journal screening using miR-libraries in cancer cell lines, and identified more than 20 novel TS-miRs
of Human Genetics. Also, editorial board members of Molecular Cytogenetics in various cancers. Among those, miR-634 activates the apoptotic pathway by directly
and Cancer Biology. He has published numerous research papers in renowned concurrent targeting of genes associated with mitochondrial homeostasis, anti-apoptosis,
international and National Peer-reviewed Journals and guided several Master antioxidant ability, and autophagy. The enforced expression of miR-634 remarkably enhanced
and Doctorate studies as well. chemotherapy-induced cytotoxicity in cancer cells in vitro and in vivo, suggesting that
concurrent miR-634–mediated modulation of cytoprotective mechanisms may be useful for
cancer therapy. More recently, we identified miR-3140, which directly suppresses BRD4 as
well as CDK2 and EGFR. BRD4 mediates transcriptional elongation of the oncogene MYC
and plays a critical role in tumorigenesis in various cancers including NUT midline carcinoma
(NMC). NMC is a rare and aggressive tumor typically driven by at (15;19) rearrangement
leading to the BRD4-NUT fusion gene. miR-3140 also downregulates the BRD4-NUT chimeric
oncoprotein directly by binding to its coding sequences (CDS) in NMC cells. Several studies
have shown that small compounds of BET bromodomain inhibitors (BETis) such as JQ1 are
highly effective against various cancers, including triple negative breast cancer, pancreatic
cancer, and NMC, and many clinical trials using BETi have been started, while the acquired
resistance to BETi has emerged as a serious problem so far. Our identified TS-miR-3140 may
be a promising candidate for the development of miR-based cancer therapy in intractable
tumors including NMC and overcome resistance to BETi due to the direct repression of BRD4.
Taken together, these findings provide novel insights into the application of miR-based
therapeutics in precision cancer medicine (PCM).

Keywords:

miRNA, Cancer, miR-634, NRF2, miR-3140, BRD4.

O. 1-3 Phytochemicals may exhibit bioactivities via hormesis: roles of proteo-
stress and adaptation
Dr. Akira Murakami is currently working as a Professor, at the Department of
Food Science & Nutrition, University of Hyogo, Japan. He graduated (B.Sc, M.Sc Akira Murakami*
and Ph.D) from Kyoto University in Agrochemistry. During his doctorate degree,
he found the anti-tumor property of Lophira alata -polyphenol (a tropical African Department of Food Science & Nutrition,
medicinal plant). He also worked as Post-doctoral associate at Kyoto University School of Human Science & Environment, University of Hyogo, JAPAN
and then become Assistant Professor at Kinki and Kyoto University. His major
field of interest includes the identification of anti-inflammatory compounds in ABSTRACT
herbs and spices (their molecular mechanisms), identification of target molecules
of phytochemicals as well as studied on stress responses and hormesis. Dr. Akira Phytochemicals, the plant secondary metabolites, have been reported to have a
Murakami, published his numerous scientific findings in several International and variety of bioactivities in many studies, although action mechanisms underlying
National Journals with the citations more than 15,000. Also, received many those functions remain to be fully elucidated. We recently reported unique
national and international Awards. In addition, he also gave a talk in various mechanisms of action of zerumbone, a sesquiterpene with anti-inflammatory and
national and international conferences, congress and symposium. He guided more chemopreventive properties (1). This electrophilic agent was found to bind
than 100 graduates during his academic career and served as a reviewer for many cysteine residues of cellular proteins in a non-specific manner to induce proteo-
International Journals. stress (2). Interestingly, the cells in which zerumbone adducts were loaded were
shown to up-regulate protein quality control (PQC) systems for homeostasis,
including induction of molecular chaperones, proteasome, and autophagy (3).
More strikingly, a non-specific protein binding action of zerumbone was revealed
to partially contribute to its suppressive effects on LPS-induced up-regulation of
iNOS and IL-1β in mouse macrophage (4). Moreover, down-regulation of HSF1,
the master regulator of PQC systems, significantly attenuated those anti-
inflammatory functions (4). This presentation highlights a unique role of
hormesis in the mechanisms underlying of bioactivities of phytochemicals.

Ref: (1) Murakami A, Ohigashi H., Int J Cancer. 2007;121(11):2357-63.
(2) Ohnishi K, et al., PLoS One. 2013;8(3):e58641.
(3) Ohnishi K, et al., Biochem Biophys Res Commun. 2013;430(2):616-22.
(4) Igarashi Y, et al. PLoS One. 2016;11(8):e0161282.

Keywords:
Phytochemical, Heat shock protein, Anti-inflammation, Zerumbone, Hormesis

O. 1-4 Targeting Cancer Metabolism for Ovarian Cancer Therapy

Dr. Danny N. Dhanasekaran, is the Deputy Director of Basic Cancer Research at the 1Ji Hee Ha, 1Rangasudhagar Radhakrishnan, 1Muralidharan Jayaraman, 3Ciro Isidoro, 4Yong
Stephenson Cancer Center of the University of Oklahoma, USA. He is also an Endowed Chair Sang Song and 1,4Danny N. Dhanasekaran
and Professor of Cell Biology at the University of Oklahoma Health Sciences Center, USA. Dr.
Dhanasekaran graduated with an M.S. in Molecular Biology and Ph.D. in Biochemistry from 1Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma
the Indian Institute of Science, Bangalore, India’s most prestigious research institution for City, OK 73104; 2Università del Piemonte Orientale, Novara, Italy; 3Cancer Research
Science and Technology. He manages the Cancer Center’s Basic Research activities. Dr. Institute, Seoul National University, College of Medicine, Seoul 151-921, Korea; Department
Dhanasekaran also serves as the Director of the NIH-sponsored Center for Biomedical of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.
Research Excellence, a federal initiative to increase research infrastructure in Oklahoma. In his
work with the Department of Defense, NASA, AFRL, and DARPA, Dr. Dhanasekaran was ABSTRACT
involved in detecting and monitoring environmental toxicants, chemical agents, and water
contaminants to better protect the brave men and women who serve in our nation’s armed Ovarian cancer remains the most lethal gynecological malignancies in the world. The five-
forces. Dr. Dhanasekaran has a strong technical background in carcinogen identification, year survival rate is only 46% for the localized disease and 29% for the distant-stage disease.
chemical and biological effects of carcinogens, carcinogen exposure toxicity, and carcinogen This is primarily due to asymptomatic nature of this cancer that precludes early diagnosis
kinetics as well. Dr. Dhanasekaran has published more than 100 articles in peer-reviewed together with the lack of an effective targeted therapy. Although the primary tumor is highly
journals, serves on numerous external scientific committees, and has been routinely invited to responsive to platinum-taxane doublet therapy, the recurring tumor is highly aggressive and
present at national and international meetings. He is on the editorial board of several cancer therapy-resistant. This is further complicated by the genomic and functional heterogeneity of
research journals and has extensive knowledge experience in federal regulatory and risk cancer cells within the tumor. It is being increasingly realized that targeting the metabolic
assessment issues related to cancer promoting carcinogens and toxicants. He has been granted vulnerabilities in cancer cells can provide an effective therapeutic strategy independent of the
two patents, one of which is on the detection of chemical toxicants. Dr. Dhanasekaran also genomic and functional heterogeneity of cancer cells. To identify such metabolic
serves as a scientific and technical reviewer for several national and international research vulnerabilities in ovarian cancer, we carried out an metabolomic analysis using a panel of high-
agencies, serves as an advisor for NIH intramural funding, and he has conducted several media grade ovarian carcinoma cell lines. Our results from such analysis indicated a relative increase
interviews regarding his research and analysis. Dr. Dhanasekaran’s previous experience in the intermediates of glycolysis and glutaminolysis along with a decrease in TCA-cycle
includes a professorship at the Temple University School of Medicine, Philadelphia from 1992 intermediates in most of the tested cell lines. The results also demonstrated a critical role for
to 2009. lysophosphatidic acid, an oncogenic lipid growth factor, in inducing such a metabolic
reprogramming in ovarian cancer cells. With the use of in vivo mouse models and ovarian
cancer patient-derived cells, our results establish the clinical utility of LPA-induced metabolic
reprogramming in ovarian cancer as a potential adjuvant therapeutic target. Nutrition-based
intervention strategies targeting this pathway will be discussed.

Keywords:

Ovarian cancer, platinum-taxane, glycolysis, glutaminolysis, TCA-cycle.

O. 1-5 Targeting Precursor Lesions of Pancreatic Cancer Prevention

Dr. Chinthalapally V. Rao, he is Kerley-Cade Endowed Chair in Cancer C.V. Rao, Ph.D
Research, and George Lynn Cross Professor of Medicine, in Medical Oncology
Section, and Director of the Center for Cancer Prevention and Cancer Drug Center for Cancer Prevention and Drug Development, Department of Medicine, Hem-Onc
Development (CCPDD) at the University of Oklahoma Health Sciences Center Section, Stephenson Cancer Center, University of Oklahoma Health Sciences Center,
(OUHSC) in Oklahoma City. His major research focus on Molecular and Oklahoma City, OK 73104, USA
Preclinical Approaches to Clinical Prevention of Colorectal and other Aero-
digestive Tract Cancers. Previously he held the position of Chief, Division of ABSTRACT
Nutritional Carcinogenesis and Leader of Chemoprevention Program at the
American Health Foundation (AHF)-Cancer Center, an NCI-designated Cancer Pancreatic cancer has the worst prognosis and it is lethal to almost all of the patients diagnosed.
Center, Valhalla, New York. Dr. Rao joined AHF-Cancer Center in 1988 as a Lack of early detection and effective interventions are major factors contributing to the poor
Senior Research Fellow and appointed in 1992 as a Member of American Health prognosis and dismal survival rates of pancreatic cancer (PC) patients. As with many epithelial
Foundation Cancer Center (AHF-CC) and Section Head of the Division of cancers, pancreatic pre-invasive precursors progress slowly over many years to develop to
Nutritional Carcinogenesis; and by 2001, he came up through the ranks to become invasive cancers in humans. Animal models that recapitulate the molecular pathology of human
Division Chief, and Cancer Center Program Leader of Chemoprevention and pancreatic intraepithelial neoplasia (PanINs) and Intraductal papillary mucinous neoplasms
Nutritional Carcinogenesis. Dr. Rao has over 245 peer reviewed research (IPMNs) their progression to pancreatic ductal adenocarcinoma (PDAC) are ideal models for
publications in leading scientific and cancer research journals. Dr. Rao is a understanding PC progression and developing treatment strategies. Recently we and others
member of the Board of Scientific Counselors/Reviewer of the National Cancer have optimized genetically engineered mouse (GEM) models with selective mutation (KrasG12D
Institute, and was a member of Chemo/Dietary Prevention Study Section (CDP), or KrasG12V) for the pancreatic cancer prevention and treatment. To identify putative targets of
Cancer Biomarkers Study Section (CBSS) and Metabolic Pathology Study pancreatic lesion development and targeted drug strategies for PDAC prevention, we applied
Section (MP) at the National Institutes of Health (NIH). transcriptomic approaches to identify novel druggable targets in transgenic KrasG12D mouse
model and human PC tumors. These include inflammasome pathway P2X7R, gastrin signaling
CCK2R, and core mucin-synthesizing enzyme GCNT3. Aberrant GCNT3 expression was also
associated with increased mucin production, aggressive tumorigenesis, and reduced patient
survival, and CRISPR-mediated knock-out of GCNT3 in PC cells reduced proliferation and
spheroid formation. Using in silico small molecular docking simulation approaches, we
identified talniflumate as a selective inhibitor GCNT3. In addition, CCK2R inhibitors
significantly suppressed PanINs progression to PDAC in KrasG12D mice. Whereas, no such
preventive effects on PDAC were observed with P2X7R inhibitors. Importantly, EGFR
inhibitor, gefitinib, significantly suppress the IPMNs progression to IPMN adenoma and
further to PDAC in KrasG12D.SMAD4-/- mice. Collectively, our findings suggest that targeting
PanINs and IPMNs through GCNT3, CCK2R and EGFR may improve PC prevention and
treatment strategies.

Keywords:

Pancreatic cancer, human pancreatic intraepithelial neoplasia, Intraductal papillary mucinous
neoplasms, mucin-synthesizing enzyme GCNT3.

O. 2-1 Personalized nutrition in postmenopausal obesity

Dr. Yong Sang Song, is a Professor at the Department of Obstetrics and In Sil Park1, Yong Sang Song2*
Gynecology, Seoul National University (SNU), South Korea. He received his
doctor’s degree from Seoul National University, Korea in 1983. He worked in 1Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea
University of Wyoming, Laramie, as research fellow in 1997, and then as 2Department of Obstetrics and Gynecology, Seoul National University, Seoul, Korea
associate Professor from 2001 to 2005. He set down and became professor and
associate dean of College of Medicine, Department of Obstetrics/Gynecology, ABSTRACT
SNU and he was elected as chairman of Korean Society of Urogynecology in
2006. And in 2009, he was selected as director of Cancer Research Institute in Obesity has been steadily increasing in countries all over the world and the
2009 in SNU. In 2009, he also organized International Conference of NAPA and etiology of obesity is multifactorial. Precision medicine for anti-obesity has been
was selected as chairman of Asian Society of Gynecologic Oncology (ASGO) the paradigm shift to manage the obese patients. Several studies have reported a
Scientific Committee. In 2012, he was selected as president of Korean Society of personalized nutrition using phytochemicals on the basis of genetic, biomarker,
Cancer Prevention. In 2018, he organized international conference of ISPCM phenotypic, or psychosocial characteristics. Postmenopausal women begin to
(International Society of Precision Cancer Medicine). His major interests are store more visceral fat in abdomen and less subcutaneous fat in femoral region
molecular mechanisms of tumors, especially the role of tumor microenvironment than premenopausal young women. Visceral obesity is closely associated with
in cancer cell metabolism and chemoresistance and precision medicine in ovarian increased risk for metabolic disturbance including type 2 diabetes, hypertension
cancer. He has published more than 300 papers in SCI journals. He has received and coronary artery disease. Adipose-derived stem cells (ASCs) are a major
numerous awards Academic award (International Conference on Ovarian Cancer), source of adipocytes and fat generation. Dysregulated adipogenic differentiation
President Award on Prevention, Boryung Cancer Academic Award on Prevention in ASCs has been implicated in the pathogenesis of metabolic disorder. We will
(Korea), Albert Nelson Marquis Lifetime Achievement Award. He is also the provide an overview of the differentially expressed genes (DEG) in ASCs from
editor in chief and senior editor or member of editorial board of various scientific visceral fat to show the relationship between expression pattern of mRNA and
Journals. BMI/waist to hip ratio. Lastly, we will discuss about a personalized nutrition
using phytochemicals for prevention and treatment of visceral obesity in
postmenopausal women.

Keywords:
Post-menopause, Visceral obesity, Adipose-derived stem cells (ASCs),
Personalized nutrition

O. 2-2 Molecular Mechanisms Underlying the Potential Anti-obesity of Cocoa
Polyphenols
Dr. Amin bin Ismail is a Professor in the field of “Food Chemistry and
Biochemistry” at the Department of Nutrition and Dietetics, Faculty of Medicine Amin Ismail, Faisal Ali and Norhaizan Mohd Esa
and Health Sciences, Universiti Putra Malaysia (UPM), Malaysia. Currently he
is a Director of Center for Quality Assurance of UPM. Dr. Amin received his PhD Department of Nutrition and Dietetics, Faculty of Medicine and Health
in field of Chemistry and Biochemistry of Food at Universiti Putra Malaysia Sciences; Research Center of Excellent, Nutrition and Non-Communicable
(UPM), Malaysia. Dr. Amin’s research areas focus on exploring of health- Diseases (NNCD), Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor,
promoting properties of cocoa components, underutilised plants for human
nutrition and food composition database. Dr. Amin has graduated 15 PhD and 25 Malaysia.
MS students. To date, Dr. Amin has more than 200 publications, and his h-index
is 35 (based on SCOPUS). Currently, he is an Associate Editor for Food ABSTRACT
Chemistry and an Editorial Board Member for Journal of Functional Foods,
Journal of Bioactive Components, and other journals. Since 2012, he has been Obesity is associated with a great diversity of diseases, including diabetes,
appointed as a Visiting Researcher at the Guangxi Academy of Agricultural cardiovascular diseases and inflammation. Phenolics/Polyphenols in cocoa have
Sciences, Nanning, China. Dr. Amin is an active member of various society and been shown to normalize dyslipidemia, suggesting that their great promise for
associations including Nutrition Society of Malaysia (NSM), International correction of lipid abnormalities. In this study, rats received normal diet or high-
Society of Nutraceuticals and Functional Foods (ISNFF), American Chemical fat diet (HFD) with additional cocoa polyphenol (CP; n=10 for each group) for
Society (ACS) and Institute of Food Technology (IFT). 12 weeks. After supplementation, HFD rats (n=10/group) were treated with 600
mg CP/kg body weight/day (CP group) for 4 weeks. CP treatment significantly
(p<0.05) lowered lipid and attenuated the increases in body weights as well as
visceral fat accumulation in CP group. CP reduced lipids by reducing
triglycerides, cholesterol and free fatty acids. Microarray analysis exhibited in a
differential expression of genes in the liver and white adipose tissues. Metabolic
pathway analysis showed that genes in lipid metabolism responsible for obesity
phenotypes were regulated in the CP group. An example, in the liver, the
peroxisome proliferator-activated receptor α (PPAR- α) and carnitine
palmitoyltransferase-1 were elevated, while fatty acid synthase and 3-hydroxy-3-
methylglutaryl–coenzyme A (HMG CoA) reductase were reduced. The work
suggested that CP can ameliorate diet-induced obesity via the regulation of lipid
metabolism biomarkers and lipid accumulation, and expected to be useful natural
bioactive compounds for alleviating obesity-mediated metabolic diseases.

Keywords:

Obesity, lipids, cocoa polyphenol, PPAR- α, HMG CoA reductase.

O. 2-3 Dietary supplements for weight loss

Dr. Suwimol Sapwarobol, is currently work as an Assistant Professor at the Sapwarobol Suwimol*
Department of Nutrition and Dietetics, Faculty of Allied Health Sciences,
Chulalongkorn University, Thailand. She is a program director of nutrition and Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn
dietetics and a former head of Department of Nutrition and Dietetics, Faculty of University, 154 Rama I Rd. Wangmai, Pathumwan, Bangkok THAILAND 10330
Allied Health Sciences, Chulalongkorn university, Thailand. Dr.Suwimol
received a B.Sc. degree in Health Science and M.Sc. in Pharmacology from ABSTRACT
Thailand before graduated with doctoral degree in nutrition from School of Public
Health, Loma Linda University, California, USA. She is also a Registered In terms of nutrition, obesity is a consequence of an energy imbalance – energy
Dietitian Nutritionist (RDN), Commission on Dietetic Registration, USA. Her intake exceeds energy expenditure. Obese compared to those with a normal or
experience of working as a research assistant in many research studies including healthy weight, are at increased risk for Non-communicable diseases (NCDs).
Adventist Health Study and a clinical dietitian at San Bernardine Medical Center Combining balanced diets of lower energy density with increased levels of
provided her a novel and diverse perspective of the nutrition profession. In physical activity should be promoted in order to prevent and treat obesity.
Thailand, her current research involves in clinical and interventional study of However, diet and lifestyle changes can be difficult in some people. Dietary
dietary management in obesity and its related non-communicable diseases. supplements for weight reduction then become popular recently. It encompasses
Dr.Suwimol was also an editor of Journal of Nutrition Association of Thailand a wide variety of active ingredients and forms. The U.S. Food and Drug
and currently a reviewer for many other journals. She has published numerous Administration (FDA) classified dietary supplements as a supplement, so it
research articles in various National and International Journals. differs from over-the-counter drugs. It does not require premarket review or
approval by the FDA. Supplement manufacturers are responsible for determining
that their products are safe and their label claims are truthful and not misleading.
Manufacturers market these products with various claims, including that these
products reduce macronutrient absorption, appetite, body fat, and weight and
increase metabolism and thermogenesis. The U.S. Government Accountability
Office concluded that the effectiveness of dietary supplements for weight control
is remain inconclusive. Additional research is needed to understand the safety and
efficacy of active ingredients.

Keywords:
weight loss, dietary Supplement, obesity

O. 2-4 Insulinotropic and antioxidant peptides from the red macroalgal species
Palmaria palmata
Dr. Richard J (Dick) FitzGerald, is a professor and chair at the Department of
Biological Sciences at the University of Limerick, Limerick, Republic of Ireland. FitzGerald, R. J. 1,*, Harnedy, P.A. 1, O’Keeffe, M.B. 1, McLaughlin, C.M. 2, Parthsarathy, V.
He has over 30 years of research experience in the field of food protein 2, & O'Harte, F.P.M. 2
ingredients. His main research interests include: food protein ingredients from
animal, plant and marine sources; functional food ingredients/nutraceuticals; 1Department of Biological Sciences, University of Limerick, Limerick, Republic of Ireland.
bioactive peptides from food protein hydrolysates; food protein 2 The SAAD Centre for Pharmacy & Diabetes, School of Biomedical Sciences, Ulster
chemistry/biochemistry; enzymatic hydrolysis of food proteins; enzymatic cross- University, Coleraine, BT52 1SA, Northern Ireland.
linking of food proteins; enzymatic debittering of food protein hydrolysates; the
technofunctional properties of food proteins and their enzymatic hydrolysates. He ABSTRACT
has published over 250 peer reviewed publications including 20 invited chapters
in books. Dr. FitzGerald has acted as overall scientific co-ordinator of 3 EU- Palmaria palmata has been utilised for centuries in many cultures as a sea
funded multidisciplinary projects in biofunctional food protein ingredients and vegetable. This macroalgae can contain up to 35% (w/w) protein and
has been an invited expert panellist to the European Food Safety Authority on consequently could act as an abundant source of bioactive peptides (protein
bioactive peptides. He has received various research funding awards from the EU, hydrolysate) for human health enhancement especially for glycaemic
Enterprise Irish, national and international food companies, the Irish Department management. Peptides were shown to exhibit insulin, glucagon like peptide-1
of Agriculture Food and the Marine as well as the Marine Institute. He has acted (GLP-1) and glucose dependent insulinotropic polypeptide (GIP) secretory
as board member for the Food for Health Ireland dairy functional foods research activity from BRIN-BD11 (pancreatic) cells, GLUTag and STC-1
initiative. He is a member of the editorial team at the Journal of Functional Foods (enteroendocrine) cells, respectively along with GLP-1 protective, dipeptidyl
and the Journal of Food Bioactives. He is a recipient of the Excellence in peptidase-IV (DPP-IV) inhibitory and antioxidant activity. The acute glycaemic
Research Award from the University of Limerick. effects of three peptides, Ile-Leu-Ala-Pro, Leu-Leu-Ala-Pro and Met-Ala-Gly-
Val-Asp-His-Ile, were also assessed in vivo using normal mice. Intake of Leu-
Leu-Ala-Pro induced a significant reduction in blood glucose while Leu-Leu-Ala-
Pro and Ile-Leu-Ala-Pro induced significant increases in plasma insulin. The
DPP-IV inhibitory and antioxidant (oxygen radical capacity assay) activity of the
three peptides appeared to be unaffected by simulated gastrointestinal digestion.
Met-Ala-Gly-Val-Asp-His-Ile possessed the highest in vitro antioxidant activity.
These in vitro and in vivo results demonstrate the potential of P. palmata- derived
peptides for the management of hyperglycaemia.

Keywords:

Antioxidant, glycaemic management, peptides, type 2 diabetes.

O. 2-5 Potential functional ingredient of rice bran to prevent lifestyle
related diseases
Dr. Ardiansyah Michwan is Associate Professor at Department of Food
Technology, Faculty of Engineering and Computer Sciences, Universitas Bakrie, Ardiansyah Michwan
Jakarta, Indonesia. He obtained his PhD and continued his research as
Postdoctoral fellowship supported by MEXT and JSPS in the Laboratory of Department of Food Technology, Universitas Bakrie, Jakarta, Indonesia
Nutrition, Graduate School of Agricultural Science, Tohoku University (2003-
2012). During this period, he continually focused on novel functional ingredients ABSTRACT
in enzyme-treated rice bran for the prevention of lifestyle-related diseases in
SHRSP. His career started as Lecturer at Universitas Bakrie (2012). In 2014, he Currently there are much effort have been focusing on the treatment for
received Award for First Rank of Outstanding Lectures in Jakarta area. He is prevention of lifestyle-related diseases. These diseases have become a significant
appointed as secretary general of ISFFN (P3FNI) and IAFT (PATPI), member at problem in recent year, especially in developed countries. Rice bran (RB) is a by-
the Division of Information and Publication in the Food and Nutrition Society of product of rice milling process, which is produced during the process of brown
Indonesia (PERGIZI PANGAN), member at the Division Education and Training rice into polished rice. Up to now, RB have many evidences due to active
of Indonesian Food and Beverage Association (GAPMMI), and member of compounds such as amino acid, fiber, -oryzanol, tocopherols, and tocotrienols.
American Society for Nutrition (ASN). His research is currently focusing on In these studies, new phytochemical components of enzymatic-treated fraction of
functional food especially functional properties of rice bran and its products. RB were identified and analysed its function in stroke-prone spontaneously
hypertensive rats (SHRSP). We have developed a RB stabilization process by
single screw extruder method. The method showed that RB can stabilize with less
-oryzanol or -tocopherols lost and have a good oxidative stability. To improve
functional properties of RB, a fermentation process have been conducted using
Rhizopus oligosporus, R. oryzae, and its combination. Fermented RB enhance the
total phenolic content, antioxidant capacity, and improved blood pressure and
glucose metabolism after 6 h administration in SHRSP. These studies may
provide opportunities to promote the production of RB as functional food
ingredient with enhanced bioactivity.

Keywords:
antioxidant, fermentation, functional ingredients, lifestyle-related diseases, rice
bran, SHRSP

O. 2-6 The use of flour from Dioscorea opposita in bread technology for
preventive purposes
Dr. Golovinskaia Oksana Vladimirovna was currently working as Assistant
Professor of the Department of Food Biotechnology of the University, Saint Golovinskaia Oksana1*, Meledina Tatiana2, Amirova Ellie3
Petersburg State University of Information, Technologies, Mechanics and Optics
(ITMO University), St. Petersburg, Russia. Dr. Oksana, received her PhD in 1,2 Saint Petersburg State University of Information Technologies, Mechanics and Optics,
Biotechnology of food products and biologically of active substances from Faculty of Food Biotechnology and Engineering, 197101, Kronverksky prospect, 49, Saint
Saint Petersburg State University of Refrigeration and Food Engineering, Russia. Petersburg, Russia; 3Pirogov Russian National Research Medical University (RNRMU),
Then Joined as senior Lecturer at ITMO University and eventually become Department of Medical Rehabilitation, 117997, str. Ostrovityanova, 1, Moscow, Russia.
Assistant Professor. In 2016, she moved to Taiwan and Worked as a Post-
Doctoral Fellow at Chung Shan Medical University and she worked on Clinical ABSTRACT
trial with several nutraceutical/ Functional Foods. Her field of interests lie in the
field of food biotechnology, the creation of new technologies for bakery products The effect of yams flour (Dioscorea opposite) on the processes of dough, physical, chemical
and beverages, the development of functional and specialized food products. She and organoleptic properties of wheat bread was studied with the aim of developing a new
teaches both undergraduate and master students during her tenure. She is a variety of functional bread. The object of the study was the flour from yams, the grinding
Secretary of the Board of the Faculty "Food Biotechnology and Engineering" and fineness of which was 250 microns. Products were investigated in the recipe of which wheat
a full member of the International Academy of Refrigeration. She has published flour was replaced with yams flour in the amount of 15, 20 and 25%. Bread was baked in a
more than 30 research articles in National and International Journals. straight-through method, and bread made from high-grade wheat flour without adding yams
flour was used as a control sample. It was established that the main component of yam flour
was non-starch carbohydrates (65%). Yams flour contained little protein (1.5%) and was gluten
free. The study of the processes of dough showed that the maximum amount of CO2, regardless
of the content of yams flour in the bread recipe, was reached after 1.5 hours. It was established
that the gas holding ratio did not depend on the amount of yams flour in the bread formulation.
It was revealed the relationship between the amount of yams flour in the recipe, the time
required to achieve the desired acidity of the dough, the height of its rise, as well as the
compressibility and porosity of the finished products. Increasing the amount of flour in the
recipe from 0 to 25% reduced the porosity of bread by 30% and the specific volume by 60%.
The recipe and technology of bread with the addition of yams flour was justified. It was proven
that adding yams flour led to a reduction in the time of the dough technological process. When
replacing 15% of wheat flour with yams flour, bread had good organoleptic properties and can
be recommended for type 2 diabetic patients.

Keywords:

Wheat bread, yams flour, Dioscorea opposita, gas formation, gas retention

O. 3-1 Natural compounds for new personalized targeted anti-cancer strategies

Dr. Claudia Cerella, is currently a Research scientist and Cell Death Team Claudia Cerella1*, Esma Yagdi Efe1, Mareike Kelkel1, Marc Diederich2
Leader at the Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, 1Laboratoire de Biologie Moléculaire et Cellulaire du Cancer (LBMCC), Hôpital
Fondation Recherche sur le Cancer et les Maladies du Sang, Hopital Kirchberg, Kirchberg, 9, rue Edward Steichen, 2540 Luxembourg, Luxembourg; 2College of
Luxembourg. She received her Master in Biology (2000) and her PhD in Cellular Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea
and Molecular Biology (2005) at the University of Tor Vergata (Rome, Italy).
After training at the Occupational Medicine, PTV (Rome, Italy), she moved to ABSTRACT
Prof. Diederich’s Laboratory for Molecular and Cellular Biology of Cancer
(LBMCC), Kirchberg Hospital, Luxembourg, first as postdoc (2007-2011), then With the advent of personalized medicine, natural compounds have become master keys in
as ResearcherCell Death Team Leader (2012-today). In 2016, she spent 6 months cancer research. Their action mechanisms predict the druggability of relevant molecular targets;
at the College of Pharmacy, Seoul National University, South Korea; and trained differential cell type-dependent susceptibility to them unveil cancer-type-specific resistance
at the Oncopole Toulouse, France (2 months). Her research interests cover cell correlated with critical mutations; the identification of responders allows the designation of
death mechanisms and novel targeted strategies circumventing cancer chemo- predictive therapeutic biomarkers. Here we take diallyl tetrasulfide DATTS (from garlic) and
resistance as well as identification of novel compounds targeting inflammation its hemi-synthetic derivative dibenzyl tetrasulfide (DBTTS) as paradigmatic examples. We
for chemopreventive and chemotherapeutic purposes. She has guided and trained discovered tubulin as direct target: their reversible binding compromises the microtubule
more than 10 PhD scholars, 6 Post-Docs and 7 master students. She also machinery, inducing mitotic arrest and apoptosis. Using hematological and colorectal cancer
published more than 80 peer-reviewed Journals including one book chapter. Also, (CRC) cell models, we identified Bcl-2 multiphosphorylation and autophagy interference as
presented her work in various international and national conferences/congress. early downstream stress response. In hematological cancer, Bcl-2 proteolysis/inhibition
promotes cell death. In CRC, the inhibition of the autophagic flux correlates with the prolonged
mitotic block and reduced susceptibility to death. This modulation occurs in KRAS-mutated
HT-29 (resistant) but not BRAF-mutated SW480 and SW620 (sensitive). Autophagy
impairment leads to accumulation of p62 protein in HT-29 cells; genetic p62 inhibition restores
sensitivity. We confirmed the translational potential of DBTTS in vitro and in vivo 3D CRC
cell models. These findings point at differential autophagic capacities as determinant for the
vulnerability to DATTS/DBTTS. Overall, potential genetic backgrounds/aberrations as
predictive therapeutic markers.

Keywords:
Diallyl compounds, mitotic arrest, apoptosis, Bcl-2, autophagic flux.

O. 3-2 Metabolites of polyphenols in cancer cells

Dr. Jianbo Xiao, is currently working as a Professor at Institute of Chinese Jianbo Xiao*
Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine,
University of Macau, Taipa, Macau, China. Dr. Jianbo Xiao, obtained his PhD in 1 Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese
nutritional science from Okayama Prefectural University, Japan (2009). He Medicine, University of Macau, Taipa, Macau, China
worked as Humboldtian at University of Wuerzbug, Germany (2013-2015), prior
to join University of Macau in 2015. Prof. Xiao has been selected as 2016 and ABSTRACT
2017 Clarivate Analytics Highly Cited Researcher (HCR) in agricultural science.
Prof. Xiao is currently the editor in chief of e-Food, the associate editor of Dietary polyphenols are most important phytochemicals in our diets. The function,
Phytochemical Analysis (Wiley) and Journal of Berry Research (IOS), the bioactivities, and pharmacology of polyphenols have been widely investigated.
editorial boards of several international journals including Critical Reviews in We have comprehensively explored the structure-stability relationship of natural
Food Science and Nutrition, Food Chemistry, Food and Chemical Toxicology, polyphenols in DMEM medium without cells. Polyphenols with catechol or
Phytomedicine, Industrial Crops and Products, and so on. He was a chairman of pyrogallol structure were evidently instable in DMEM medium without cells. We
2015 International Symposium on Phytochemicals in Medicine and Food (- further investigated the metabolites of quercetin and luteolin when incubated with
ISPMF2015) organized by PSE and its second edition (2-ISPMF 2017), third several cells (cancer and normal) at 37 °C in 5% CO2 for 48 h. The metabolites
edition (3-ISPMF 2018). His major research work focuses on bioactives/ of quercetin were found to be the glucuronide, sulfate and methylate of its
nutraceuticals against various metabolic syndrome especially cancer and diabetic oxidized quinone. The metabolites of luteolin were found to be its glucuronide,
mellitus. Prof. Xiao has published over 150 research papers in various Peer- sulfate and methylated form. The metabolites were quite different from different
reviewed Journals. cells. We futher investigated what really happened for polyphenols in A549 cell,
HepG2 cell and Caco-2 cell.

Acknowledgements: This work was financially supported by Multi-Year
Research Grant of University of Macau (MYRG2018-00169-ICMS) and Start-up
Research Grant from University of Macau (SRG2015-00061-ICMS-QRCM).

Keywords:
Polyphenols, metabolites, quercetin and luteolin, cancer cells.

O. 3-3 Combinatorial Approaches with Phytochemicals for Prevention of Cancer

Dr. John DiGiovanni, is currently Professor in the Division of Pharmacology John DiGiovanni,*
and Toxicology, College of Pharmacy at the University of Texas at Austin. He
currently holds the Coulter R. Sublett Endowed Chair in Pharmacy. In addition, 1 Division of Pharmacology & Toxicology and Department of Nutritional Sciences, The
he is the Director of the Center for Molecular Carcinogenesis and Toxicology and University of Texas at Austin, 1400 Barbara Jordan Blvd, Austin, Texas 78723
Associate Director for Basic Research in the LiveSTRONG Cancer Institutes at
the University of Texas. Dr. John DiGiovanni, received his B.S degree in ABSTRACT
Pharmacy and his Ph.D degree in Pharmacology from the University of
Washington, Seattle, Washington. He did his postdoctoral work at the McArdle Several studies have shown that combinations of chemopreventive agents can
Laboratory for Cancer Research, University of Wisconsin, Madison, WI in produce additive and even synergistic cancer inhibitory effects. In addition, using
carcinogenesis and cancer biology. After joining the University of Texas MD combinations of agents may produce overall effects that more similarly mimic
Anderson Cancer Center in 1983 and became the Director of the Science Park- that of calorie restriction, a very broad acting cancer preventive modality. Our
Research Division and Chair of the Department of Carcinogenesis in 1997 until initial studies have evaluated combinations of ursolic acid (UA) with resveratrol
he joined the University of Texas at Austin in January of 2010. He received (RES) or curcumin (CURC) using the two-stage skin carcinogenesis model. Both
numerous awards including NSF-URP Research Awards, Rho Chi Award, combinations given topically during the tumor promotion stage produced an
Faculty Research Award and recently with The University of Texas Co-op approximately additive inhibitory effect at the doses tested which correlated with
Society Career Research Excellence Award. Dr. DiGiovanni has published more increased inhibition of multiple epidermal signaling pathways, decreased
than 320 research articles (including peer-reviewed research articles and book keratinocyte transcription factor function and reduced skin inflammation. In other
chapters). He is an Editor-in-Chief of the journal Molecular Carcinogenesis. studies, we have recently developed a novel two-tiered screening approach using
ATP depletion (high throughput primary screen) in cancer cells followed by
analysis of combinations identified in the primary screen by high-throughput
analysis of their effects on glutamine uptake (secondary screen) in the same cells
for identifying synergistic combinations of phytochemicals for cancer
chemoprevention. This approach has initially focused on prostate cancer and uses
a novel allograft model with a tumor cell line (HMVP2 cells) derived from HiMyc
mice. Several synergistic phytochemical combinations have been identified using
this approach that will be discussed in detail. Collectively, the current results
demonstrate the effectiveness of several combinations of agents for inhibition
tumor development in two different mouse models of cancer. The greater
effectiveness of the combinations for inhibition of tumor development was
associated with greater ability to inhibit critical signaling events associated with
cell proliferation/survival and inflammation during tumor development/growth.
These data will be discussed in the context of developing strategies for combining
agents for the most effective chemoprevention activity.

Keywords:

Chemopreventive agents, resveratrol, curcumin, prostate cancer.

O. 3-4 Phytochemical Compositions and Anti-cancer Activities of Butterfly Pea
(Clitoria Ternatea) Seed and Petal
Dr. Zhimin Xu, is a Professor at School of Nutrition and Food Sciences of
Louisiana State University, USA. His research interests include 1) extraction, Zhimin Xu,
isolation, and characterization of health promoting compounds in agricultural and
food products; 2) utilization of the health promoting compounds to improve food School of Nutrition and Food Sciences, Louisiana State University
quality and value; 3) characterization and determination of flavor volatiles and
colorants in food products and their changes during food processing; 4) food ABSTRACT
microconstituent and adulterant analysis using chromatography technique. He
also teaches chromatography analysis and food flavor and colors courses. He had Butterfly pea (Clitoria ternatea) is a member of Fabaceae family and Papilionaceae sub-family.
conducted and accomplished the research grants from national organizations, It has been reported to possess health promoting functions (owing to rich in anthocyanins)
prestige companies, and local research boards. He published a number of including anti-diabetes, anxiolytic, anti-convulsant, antipyretic, anti-inflammatory and
refereed articles and book chapters and edited book of “analysis of antioxidant- analgesic functions. In this study, lipophilic and hydrophilic extracts of butterfly pea seeds and
rich phytochemicals”. He was awarded the 2007 Most-Cited Paper of Journal of petals were extracted by non-polar and polar solvents, respectively. The hydrophilic phenolics,
Food Science. He was invited to give presentation at international conferences, lipophilic tocopherols, phytosterols and fatty acids in seeds and petals were identified and
universities and institutes every year. He was the member-at-large of the Food quantified by HPLC, LC-MS or GC method for exploring and understanding the potential
Chemistry Division of Institute of Food Technologists. Dr. Xu was also invited bioactive composition and distribution in butterfly pea. Fifteen antioxidant phytochemicals
as an external reviewer for European Research Council, Sweden International were found in the seeds. Among them, sinapic acid, epicatechin and hydroxycinnamic acid
Foundation for Science, Chinese and Swiss and Romanian National Science derivative were above 0.5 mg/g of fresh weight. The petals had a group of ternatins, flavone
Foundations. glycosides and delphinidin derivatives. Both of the seeds and petals contain four phytosterols
and alpha- and gamma- tocopherol. The level of beta-sitosterol or gamma-tocopherol in the
seeds was much higher than in the petals. Linoleic acid was the most abundant fatty acid in
the seeds and petals, while phytanic acid was only found in the petals. The capabilities of
lipophilic and hydrophilic extracts of the seeds and petals in inhibiting HEp-2 human
carcinoma cell viability were evaluated in this study as well. The inhibitory capability of seed
or petal hydrophilic extract was much higher than either seed or petal lipophilic extract, while
the capability of seed hydrophilic extract was significantly higher than the petal hydrophilic
extract. The results of this study indicate that butterfly pea seed and petal are rich in different
antioxidant phytochemicals and could be utilized as a health promoting food ingredient or
supplement for the prevention of cancer development.

Key words:
phytochemicals; ternatins; tocopherols; phytosterols; lipids; antioxidants

O. 3-5 Are we ready for precision medicine using berries?

Dr. Li-Shu Wang, is an Associate Professor of Medicine, Medical College of Pan Pan, Hayden Krause, Arielle Baim, Lyndsey Runaas, Laura Michaelis,
Wisconsin. She received her Ph.D. in Veterinary Biosciences in the College of Ehab Atallah, Li-Shu Wang
Veterinary Medicine at Ohio State University in June, 2006 and worked as a Post-
Doctoral fellow and research scientist in the same University. Dr. Wang’s Division of Hematology and Oncology, Department of Medicine, Medical College of
expertise is in colorectal cancer (CRC) biology and prevention in animals and Wisconsin, Milwaukee, Wisconsin, USA
humans. The major goal of Dr. Wang’s research is to translate the findings from
bench to bedside. Using bio-directed fractionation, Dr. Wang showed that the ABSTRACT
anthocyanins in black raspberries (BRBs) are important for their
chemopreventive effects and she provided evidence that the ellagitannins may be Familial adenomatous polyposis (FAP) is characterized by the early onset of colonic polyposis
less important. More importantly, she has evidence that BRBs cause and a high risk for colorectal cancer. Our group conducted a human clinical study to determine
demethylation of tumor suppressor genes in rodent and human colon leading to whether black raspberries (BRBs) might regress rectal polyps in patients with FAP. Fourteen
their enhanced expression in two human clinical trials. Currently, she is an patients with FAP were treated with BRBs daily for 9 months. Despite the encouraging finding
editorial member for Journal of Scientific Report, Berry Research and e-Food that a 9-month BRBs intervention decreased polyp burden in 11 of 14 patients with FAP, 3
Journal. She is an active member of American Chemical Society and American patients were non-responders. Mechanistic studies suggest that non-responders were resistant
Association for Cancer Research. She also published more than 60 research to BRBs-induced hypomethylation. Myelodysplastic syndromes (MDS) are a group of bone
articles in high impact Journals with 6 book chapters. marrow disorders characterized by progressive cytopenias and progression to acute myeloid
leukemia [8]. Hypomethylating agents (HMAs), such as azacitidine and decitabine, are FDA-
approved therapies for MDS patients [9-10]. Approximately 50% of MDS patients respond to
HMAs. Although both azacitidine and decitabine are effective therapies for some MDS patients,
they are associated with significant toxicities that place a large burden on MDS patients and
affect their quality of life. We conducted a pilot clinical trial to evaluate the hypomethylating
properties of BRBs in patients with low-risk MDS. Study is still ongoing, we have recruited 16
patients. Data from 7 patients suggested that 3 months of BRB intervention induced
hypomethylation in 6 of 7 patients with MDS, 1 patient was a non-responder. Therefore, our
data suggest that changes of methylation may be used as indicators for responsiveness to BRBs
intervention that warrants investigation in a larger clinical trial. It would be ideal to identify
individuals who are likely to benefit from a BRB intervention before recruiting subjects into a
clinical trial.

Keywords:

Familial adenomatous polyposis, black raspberries, hypomethylation, Myelodysplastic
syndromes.

O. 4-1 Effect of the camellia oil on the alleviation of aluminum-induced cognitive
decline
Dr. Gow-Chin Yen, is presently a Chair Professor of the Department of Food
Science and Biotechnology at the National Chung Hsing University, Taiwan. Dr. Ming-Hung Weng, Zih-Ying Li, Gow-Chin Yen
Yen is an internationally renowned expert in molecular food chemistry, food
safety and functional foods. His current research interests focus on beneficial Department of Food Science and Biotechnology, National chung Hsing University, 145 Xingda Road,
effects and molecular mechanisms of food bioactives in health promotion and Taichung 40227, Taiwan
disease prevention targeted at oxidative stress, cancer, inflammatory diseases. Dr.
Gow-Chin Yen, has received several awards and professional recognitions ABSTRACT
including a Fellow of the Division of Agricultural and Food Chemistry of the
American Chemical Society, a Fellow of the International Academy of Food The pathogenesis of Alzheimer’s disease (AD) is associated with various
Science and Technology, a Fellow the Royal Society of Chemistry and a Fellow causative factors such as oxidative stress, inflammation, gut microbiota (GM)
of Institute of Food Technologists. He is an editor-in-chief of Journal of Food and imbalance, and environmental exposure to aluminum. Camellia oil has been
Drug Analysis, and member of editorial boards of Molecular Nutrition and Food reported with beneficial effects in promoting health. This study was to investigate
Research, Journal of Agricultural and Food Chemistry, Journal of Food Science, the effect of camellia oil on the alleviation of cognitive decline. The Sprague-
etc. Dr. Gow-Chin Yen, has published more than 100 research papers in peer Dawley rats were administered aluminum chloride by oral gavage and with or
reviewed Journals. He also guided several master and Doctorate students. without camellia oil for 7 weeks. The rats were undergone the water-maze task
on day 46 after the gavage and were scarified on day 50. The results revealed that
camellia oil alleviated memory and learning deficits, enhanced the activity of
antioxidant enzyme, and regulated the expression of immunity-related cytokines
in rat brain. Camellia oil also decreased the expression of AD-related proteins in
rat brain. Results from next-generation sequencing indicated that camellia oil
elevated the alpha-diversity of GM and the richness of probiotics. In summary,
camellia oil can improve memory and learning deficits in behavioral tests,
decrease oxidative stress and neuroinflammation, ameliorate AD biomarkers in
rat brain, and enrich the GM diversity and modulate GM composition. These
effects demonstrate that camellia oil could be a dietary bioactive for promotion
cognitive health.

Keywords:

Alzheimer’s disease, aluminium, Camellia oil, gut microbiota, cognitive health.

O. 4-2 An alcohol-derived metabolite salsolinol induces hepatic damage in mice

Dr. Hye-Kyung Na, is a Chair of Department of Food Science & Biotechnology, Hyunjung Choi1, Xiancai Zhong2, Jeong-Eun Lee1, Jae Min Lim3, Kwang Pyo Kim3, Young-
College of Knowledge-Based Services Engineering, Sungshin Women’s Joon Surh2, Hye-Kyung Na1*
University, Seoul, South Korea. She obtained PhD degree at the Department of
Food & Nutrition, Chonnam National University, South Korea and had 1Department of Food Science and Biotechnology, College of Knowledge-Based Services
Postdoctoral training at the Pediatrics and Human Development, Michigan State Engineering, Sungshin Women’s University, Seoul 01133, Republic of Korea. 2Tumor
University. Since relocation to Korea in 2000, Prof. Na joined to the lab of Prof. Microenvironment Global Core Research Center, College of Pharmacy, Seoul National
Young-Joon Surh in Seoul National University, College of Pharmacy where she
worked as Associate Principal Researcher followed by Research Assistant University, Seoul 08826, Republic of Korea. 3Department of Applied Chemistry, College of
Professor until 2008. Prof. Na received the International Scholar-in-Training Applied Science, Kyung Hee University, Yongin 17104, Republic of Korea
Award given by American Association for Cancer Research consecutively from
2003 to 2007. She currently serves as Associate Editor for the Molecular ABSTRACT
Carcinogenesis, Toxicological Research and Journal of Cancer Prevention and as
Editorial Board member for Journal of Functional Food, Free Radical Research Alcohol consumption is one of the causes of liver cancer as well as chronic liver diseases. In
and Molecules and Cells. Prof. Na’s research focuses on molecular mechanisms this study, we determined whether salsolinol (SAL) could be implicated in the pathogenesis of
underlying cancer chemoprevention with dietary and medicinal phytochemicals liver damage in C57BL/6 mice. First, we confirmed that the serum level of SAL was found to
and role of 15-hydroxyprostaglandin dehydrogenase and its metabolite 15-keto be significantly increased in acute ethanol-treated male compared with female mice as
PGE2 in breast and colon carcinogenesis. Currently her research focuses on effect determined by tandem mass spectrometry analysis. Next, male and female C57BL/6 mice were
of alcohol metabolites on liver damage and genes differences. She published intraperitoneally treated with either vehicle or SAL (200 mg/kg) for 4 or 8 weeks. SAL
numerous research articles in various renowned peer-reviewed Journals. increased the mRNA level of MCP-1 and Col1A1 to a greater extent in male mice than female
ones. The male mice treated with SAL exhibited more extensive infiltration of inflammatory
mononuclear cell infiltration and severe liver damage than female mice as determined by
hematoxylin and eosin staining. Moreover, SAL markedly increased the mRNA expression of
NADPH quinone oxidoreductase-1 compared with that in female mice. SAL also upregulated
mRNA expression of antioxidant enzymes, such as superoxide dismutase 1/2, glutathione
peroxidase, and catalase as well as their major regulator Nrf2. Further, there was a substantial
increase of ataxia-telangiectasia mutated and its substrate H2AX, indicative of DNA damage
promoted by SAL, which was higher in male mice than female. In another experiment, SAL
treatment resulted in enhancement of expression of androgen receptor (AR), an initiation
marker of hepatocellular carcinogenesis, to a greater extent in male than female mice. These
findings suggest that SAL may contribute to alcohol-induced liver damage and cancer by
inducing DNA damage, possibly through a gender-dependent expression of NQO1 and AR.

Key words:

Alcohol metabolite, Salsolinol, Gender difference, Liver cancer.

O. 4-3 Polyphenolic Antioxidants Sensitize the Inactivation of Tyrosine
Phosphatases by UV Radiation
Dr. Zhizhuang Joe Zhao, is currently working as a Professor at the Department
of Pathology, University of Oklahoma Health Sciences Center, Oklahoma, USA. Zhizhuang Joe Zhao
He is also the Director of Experimental pathology and pathology Graduation
Program at University of Oklahoma Health Sciences Center, Oklahoma, USA. Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma, USA
He received his PhD from the Department of Biochemistry and Biophysics,
Oregon State University, Oregon, USA under the Guidance of Dr. Sonia ABSTRACT
Anderson. He did his Post-Doctoral Training at the Department of Biochemistry,
University. of Washington, Washington, USA and later he joined as a Research Protein tyrosine phosphatases (PTPs) constitute a large and structurally diverse family of
Assistant Professor in the same institution. Then he moved to Vanderbilt enzymes. They play both negative and positive roles in cell signaling, and deregulation of
University, Tennessee, USA and become Associate Professor. In 2005, he again PTPs has major pathological implications. PTPs contain a conserved cysteinyl residue in the
moved to his current University (University of Oklahoma Health Sciences Center) catalytic site that is vulnerable to oxidation. They can be oxidized and thereby inactivated in
and become full professor. His research interest foci on the involvement of vitro and in living cells by reactive oxygen species generated by various stimuli including
protein tyrosine phosphatases in various signal transduction pathway and various ultraviolet (UV) radiation. Antioxidants can presumably prevent such oxidation and thus
kinases activities in cancer (especially in hematologic cancer), diabetes and aging. maintain PTP catalytic activity. Indeed, we found that many thiol antioxidants prevented and
He has published numerous scientific articles in various international peer- reversed UV-induced inactivation of PTPs. Surprisingly, however, polyphenolic antioxidants
Reviewed Journals. He has guided numerous graduates/scholars and has been markedly enhanced UV-induced PTP inactivation. These compounds apparently act as
invited as an external reviewer for European Research Council, US research photosensitizers by accelerating the oxidation rate and increasing the degree of PTP
council etc. He is the Editorial Board Member of numerous international Cancer inactivation. Our data reveal that antioxidants can have opposite effects on PTP activities.
Journals. They may have both beneficial and adverse effects on human health dependent on the nature
of oxidizing reagents and the PTPs affected.

Keywords: tyrosine phosphatases, antioxidants, polyphenols, UV radiation

O. 4-4 In Vivo Bioactivities of Oral Administration of Superoxide Dismutase and
Its Possible Mechanism
Dr. Shutao Liu, is presently working as a Professor and as well as hold a Director
position at Institute of Biotechnology, Fuzhou University, Fuzhou, China. Dr. Shutao Liu,* Xiaochao Chen, Dan Zhao, Nanhui Ye, Haibin Chen, Xiaolu Gao, Jingke Guo,
Shutao Liu, has graduated from the Fuzhou University, Fuzhou, China and Pingfan Rao
worked under the supervision of Professor Pingfan Rao (China) and Professor
Akio Kato (Japan). His research interest foci on Food and Medicine Institute of Biotechnology, Fuzhou University, Fuzhou 350108, China
Biotechnology, Free Radical Biology and Medicine to identify the food bio-
actives (functional foods/nutraceuticals) derived from food and traditional ABSTRACT
Chinese medicine (TCM) to treat digestive tract dysoeders. He has been engaged
in the research of protein chemistry and engineering since 1992. Nearly 10 years, As a potent antioxidant, superoxide dismutase is not used in food for the concern
he focused on the basic and application research of antioxidant protein and of its gastric degradation. This work aimed to investigate the in vivo bioactivity
enzyme (such as collagen and SOD), biological molecule (such as berberine), and of SOD’s oral administration and the possible mechanism. Cu/Zn-SODs were
the relationship between the structure and function of the food functional factors, orally administrated to rats of type 1 or 2 diabetes，cerebral ischemia-reperfusion
while continuing the expression, separation, purification and characterization of (CIR) and carbon tetrachloride-induced hepatitis. It had significant hypoglycemic
recombinant protein. He has guided many graduate students and PhD scholars as effect on diabetic related indexes, alleviated related index of CIR rats and
well as teaches related to Food safety, Functional Food, recent trends in Food improved their neural behaviour, restored both serum transaminases and MDA
Technologies. He has published 50+ scientific research articles in various level and attenuated the histopathological changes in rats of chronic hepatitis.
national and international Journals. He also participated in several international Only 1.0% of SOD survived after 2.5 h treatment in simulative gastric juice while
and national conferences and congress. He also serves as an Editorial Board the total increment of SOD activity was higher than that of orally administrated
member in numerous International Journals related to Food Science/Nutrition and Cu/Zn-SOD, and Mn-SOD mRNA level was found to increase in the brain, liver
Food Technology/Processing. and heart. Oral administration with SOD resulted in better hypoglycemic effect
than intragastric administration. Possibly destroyed by gastric juice, orally
administrated SOD was effective in alleviating hyperglycemia, cerebral
ischemia-reperfusion and chronic hepatitis, indicating the feasibility of SOD
application in food, and implying its action may occur by a novel mechanism in
the alimentary tract in spite of the gastric destruction.

Keywords:

SOD, type 2 diabetes, hypoglycemic, neural behaviour

O. 4-5 Immobilized β-Glucosidase for Mogrosides Deglycosylation from
Lo Han Kuo
Dr. Kuan-Chen Cheng, is currently an Associate Professor at Graduate Institute
of Food Science and Technology, National Taiwan University, Taipei, Taiwan as Hsueh-Ting Wang 1, Jin-tong Yang 1, Ting-Jang Lu 1, Kuan-Chen Cheng1,2*
well as at Institute of biotechnology, National Taiwan University, Taipei, Taiwan.
He received his Ph.D. degree in agricultural and biological engineering at The 1 Graduate Institute of Food Science and Technology, National Taiwan University, Taipei,
Pennsylvania State University, USA and moved to National Taiwan University, Taiwan. 2 Institute of biotechnology, National Taiwan University, Taipei, Taiwan.
Taiwan for Post-Doc and become assistant Professor. His field of interest
includes Fermentation, Food Biotechnology, Biomedical materials, Bioreactor ABSTRACT
design. He received many awards including Chiang-Hsu Lien-Chen Food
Technology Honorary Award (TAFST), Academic award of Agricultural An immobilized enzyme system for bioconversion of Lo Han Kuo (LHK)
Chemical Society of Taiwan. He has guided/ Supervised numerous undergraduate, mogrosides was established. β-Glucosidase which was covalently immobilized
master and doctorate students. Dr. Kuan-Chen Cheng is an active member of onto the glass spheres exhibited a significant bioconversion efficiency from
Taiwan Association for Food Science and Technology, Agricultural Chemical pNPG to pnitrophenol over other carriers. Optimum operational pH and
Society of Taiwan, the American Society of Agricultural and Biological temperature were determined to be pH 4 and 30oC. Results of storage stability
Engineers (ASABE), Taiwan Association for Food Protection, Institute of Food test demonstrated that the glass sphere enzyme immobilization system was
Technology (IFT) and the honor societies Gamma Sigma Delta (agricultural) and capable of sustaining more than 80% residual activity until 50 days and operation
Alpha Epsilon (agricultural, food, and biological engineering). He also published reusability was confirmed for at least 10 cycles. The Michaelis constant (Km) of
more than 50 research articles in many peer-reviewed Journals. the system was determined to be 0.33 mM. The kinetic parameters, rate constant
(K) at which Mogrosides conversion were determined, the τ50 in which 50% of
mogroside V deglycosylation /mogroside IIIE production was reached, and the τ
complete of complete mogroside V deglycosylation/ mogroside IIIE production,
were 0.044/0.017 min-1, 15.6/41.1 min, 60/120 min, respectively. Formation of
the intermediates contributed to the kinetic differences between mogroside V
deglycosylation and mogroside IIIE formation.

Keywords:
Lo Han Kuo, Mogroside V, Mogroside IIIE, Glucosidase, Immobilized Enzyme

O. 5-1 Natural compounds induce immunogenic cell death in cancer

Dr. Marc Diederich, currently working as a Professor of Biochemistry, College Marc Diederich
of Pharmacy, Seoul National University, Seoul, Korea. He received his PhD in
molecular pharmacology in 1994 from the University Henri Poincaré Nancy, 1Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul 151-742,
France. After training at the University of Cincinnati, USA, he focused his South Korea
research on cancer and leukemia cell signaling pathways and gene expression
mechanisms triggered by natural compounds with epigenetic-, anti- ABSTRACT
inflammatory- and cell death-inducing potential. He directs the Laboratory for
molecular and cellular biology of cancer (LBMCC) at Kirchberg Hospital in Apoptosis and autophagy were traditionally considered as the most prominent
Luxemburg. He was appointed associate Professor of Biochemistry at the College cell death or cell death-related mechanisms. By now multiple other cell death
of Pharmacy of Seoul National University (SNU) in 2012. In 2017, he was modalities were described and most likely involved in response to epigenetic
tenured and promoted to full professor at SNU. Since 1998, he is the organizer of treatments. It can be hypothesized that especially necrosis-related phenotypes
the “Signal Transduction” meetings in Luxembourg. Dr. Marc Diederich’s triggered by various treatments or evolving from apoptotic or autophagic
research focuses on the development of novel anti-cancer drugs. He and his mechanisms, provide a more efficient therapeutic outcome depending on cancer
collaborators investigated for example chalcones that are aromatic ketones, type and genetic phenotype of the patient. In fact, the recent discovery of multiple
known to exhibit anti-microbial, anti-inflammatory and anti-cancer activities. Dr. regulated forms of necrosis and the initial elucidation of the corresponding cell
Marc Diederich has published more than 215 research articles in Peer-Reviewed signaling pathways appear nowadays as important tools to clarify the
journals with 53 articles are High index status. He is currently an Editor-in-Chief immunogenic potential of non-canonical forms of cell death induction. This
for “Journal of Cancer Prevention” as well as in Editorial Board for several presentation will cover the effect of epigenetically active compounds and
international Journals like Cell stress, Genes & Nutrition and so on. highlight their activity leading to non-canonical or immunogenic cell death.

Keywords:
Apoptosis, autophagy, cancer, necrosis, cell signaling

O. 5-2 Protective effect of litchi flower proanthocyanidin against vascular
endothelial growth factor expression in hepatocellular carcinoma cells
Dr. Deng-Jye Yang, is a professor at Institute of Food Safety and Health Risk
Assessment, National Yang-Ming University, Taipei, Taiwan. Dr. Deng-Jye exposed to nickel
Yang did his PhD at National Taiwan University, Taipei, Taiwan, then worked
as Post-Doctoral fellow and become Assistant professor at Chung Shan Medical Deng-Jye Yang 1, Jau-Tien Lin 2, Yuan-Yen Chang 3, Yi-Chen Chen 4, Po-Lin Liao 1
University, Taichung, Taiwan. Later as Associate professor and full professor at
the same Chung Shan Medical University, Taichung, Taiwan. Currently, he is 1 Institute of Food Safety and Health Risk Assessment, National Yang-Ming University,
also an adjunct professor at China Medical University, Taichung, Taiwan. His Taipei, Taiwan. 2 Department of Medical Applied Chemistry, Chung Shan Medical
major research focus on cancer biology especially identification of novel
chemotherapeutic compounds. Dr. Deng-Jye Yang, also received several awards University, Taichung, Taiwan. 3 Department of Microbiology and Immunology, School of
and honors including Chen Chiang-Hsu’s Food Science and Technology Medicine, Chung-Shan Medical University, Taichung, Taiwan.
Research Award, Exceptional Talent Reward, Food Academic Research Honor
Award and Future Science and Technology Breakthrough Award. He also ABSTRACT
published more than 65 peer-reviewed Journals including 5 book chapters.
Litchi (Litchi chinensis Sonn.) flower has antioxidant, anti-inflammatory, hepato-protective,
cardiovascular protective and anti-obesity activities, and proanthocyanidins ((-)-epicatechin
and proanthocyanidin A2) are the major effective compounds. Nickel (Ni) widely used in
industry is considered to be an occupational and environmental pollutant. Liver cancer patients
are closely related to the vascular endothelial growth factor (VEGF) expression. VEGF could
be up-regulated by Ni notably. Therefore, the protective effect of litchi flower
proanthocyanidin fraction (LFP) against VEGF expression in liver cancer in the presence of Ni
was evaluated in the investigation. LFP was prepared from litchi flower through acetone extract
and silica gel column chromatography. Hepatocellular carcinoma (Hep G2) cells exposed to
Ni were incubated with various concentrations of LFP. VEGF levels were assayed through the
enzyme-linked immunosorbent assay (ELISA), and all signaling pathways were investigated
using Western blot analyses. VEGF level in Hep G2 cells could be significantly decreased
through LFP treatment. Although Ni could induce a considerable level of VEGF in the cells,
LFP treatment could also significantly lower the Ni-induced VEGF generation. The inhibition
of hypoxia-inducible factor (HIF)-1α expression by blocking phosphatidylinositol-3-kinase
(PI3K)/ protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway, and the
down-regulation of Raf-1 proto-oncogene, serine/threonine kinase (RAF1)/ mitogen-activated
protein kinase (MEK1/2) /extracellular-signal-regulated kinase (ERK1/2), and Janus kinase 2
(JAK2) /signal transducers and activators of transcription 3 (STAT3) pathways are important
molecular mechanisms for the LFP action. Our results highlight the LFP protective effect for
VEGF suppression in hepatocellular carcinoma exposed to Ni. LFP should have the potential
to act as a VEGF inhibitor to reduce the risk of liver cancer in Ni-polluted environments.

Keywords:

Nickel (Ni); proanthocyanidin; hepatocellular carcinoma cell; vascular endothelial growth
factor (VEGF); hypoxia inducible factor (HIF)-1α.

O. 5-3 Probiotic kimchi prevented colitis-associated cancer as well as H. pylori-
associated cancer
Dr. Ki Baik Hahm, is currently professor of medicine at CHA University School
of Medicine and director in CHA Cancer Prevention Research Center and director Eun A Kang1,Jeong Min An1, Ji Young Oh2, Dong Yoon Lee2, Seung Hye Choi2, Eun Ji
in Research division of CHA Univ Bundang Medical Center. He graduated from Lee4, Dong Il An4, Yong Jin Park3, Ki Baik Hahm1,4
Yonsei University at 1983 and spent three years post-doc in NCI, MD from 1997.
After serving assistant professor of Medicine at Yonsei University from 1990 to 1 CHA Cancer Prevention Research Center, CHA Cancer Institute, CHA University,
1994, he moved to Ajou University School of medicine, where he was appointed Seongnam, 2 CJ Food Research Center, Suwon, 3 GI Medics, Seoul, 4 Digestive Disease
as professor of medicine from 1994 to 2006. In 2012 he finally settled in CHA
University with prestigious appointment of research driven hospital project as Center, CHA University Bundang Medical Center, Seongnam, Korea
leader, which will be funded from government from 2013 up to 10 years. He is
performing translational research in the field of H. pylori infection, etio- ABSTRACT
pathogenesis and pathophysiology of peptic ulcer disease research, gastric
carcinogenesis and their prevention, and inflammatory bowel disease. He had As evidenced by previous publication that fermented, specially formulated, probiotic rich
published more than 170 SCI publications and am taking several researches kimch named as cancer preventive kimchi (cpkimchi) afforded significant prevention of
granted from government. He is currently appointed as president of the Korea Helicobacter pylori-associated gastric carcinogenesis. Hence, we have hypothesized cpkimchi
Society of Cancer Prevention and Society of Free radical Research. He serves as can prevent azoxymethane initiated, dextran sulfate sodium promoted- colitis associated cancer
editor to Journal of Gastroenterology and Hepatology and Journal of Digestive (CAC) and explored the underlying detailed mechanisms of chemoprevention. After 12 weeks
Disease and editor-in-chief of Clinical Endoscopy journal. Recently he added his of CAC model, cpkimchi significantly prevented CAC, whereas non-fermented kimchi did not,
filed of research in stem cell application as well as translational research to solve signifying the contribution of probiotics in these efficacies of chemoprevention. In detail, the
unmet medical needs in gastroenterology. findings that probiotics, L. plantarum, contained in cpkimchi significantly exerted inhibitory
actions of IL-6, STAT3, and NF-κB, while not in non-fermented kimchi, highlighted the
involvement of probiotics. In addition to cancer preventive actions of probiotic, gradients
contained in kimchi such as red pepper, garlic, radish, etc, exerted significant cancer preventive
actions All of these regulatory actions of cpkimchi were pivotal in either inhibiting mutagenic
TNF-α or IL-6 signaling, after which we additionally found that cpkimchi can attenuate
cachexic symptoms relevant to CAC development. Currently randomized placebo controlled
clinical trial to validate cancer preventive action of cpkimchi is under investigation in patients
with endoscopic polypectomy with 10 weeks of cpkimchi intake to document adenoma
recurrence inhibition. Common to all of these beneficiary actions of cpkimchi, profuse and
effective microbiome contributed to quite efficient way of cancer prevention. On
pyrosequencing analysis, significant changes in gut microbiota were noted in group treated
with cpkimchi. Conclusively, long-term administration of probiotic kimchi can endow
preventive outcome in patients with inflammatory bowel disease.

Keywords:
Kimchi, Cancer prevention, Dietary intervention, Colitic cancer

O. 5-4 Role and regulation of Hexokinase II in aerobic glycolysis and
cisplatin sensitivity in ovarian cancer
Dr. Benjamin Tsang, is a Full Professor of Obstetrics & Gynaecology and Cellular
& Molecular Medicine, University of Ottawa. As Director of the Reproductive Chae Young Han1,3, David A Patten2, Annie Cheung4, Yong Sang Song5,
Biology Unit and the Associate Chair (Research) in his Department, Dr. Tsang Mary-Ellen Harper2 and Benjamin K Tsang1,3*,
developed a multi-disciplinary reproductive health research program in Ottawa. He
is also a Senior Scientist, Chronic Disease Program, Ottawa Hospital Research 1 Departments of Obstetrics & Gynecology, Cellular & Molecular Medicine and 2Biochemistry,
Institute. BenTsang, received his PhD in Pharmacology at University of Ottawa, Microbiology & Immunology, University of Ottawa, 3Chronic Disease Program, Ottawa
Canada and joined as Director of Research at Ottawa Civic Hospital and moved to
Mc Gill University and worked as Adjunct Professor. Finally moved to his current Hospital Research Institute, Ottawa, Ontario, Canada, 4Department of Pathology, University
university (University of Ottawa) and promoted as full professor. He is an of Hong Kong and 5Department of Obstetrics and Gynecology, Seoul National University
internationally known for his translational research in cell fate regulation in
women’s reproductive health. His research focuses on the regulatory mechanisms ABSTRACT
of normal ovarian follicular growth and the pathophysiology of anovulatory
infertility, and the molecular and cellular basis of chemoresistance in ovarian cancer. Ovarian cancer (OVCA) is the leading cause of cancer deaths in women, but cisplatin (CDDP)
He has contributed over 215 original publications, 20 reviews/book chapters and resistance is a hurdle to successful therapy. Elevated aerobic glycolysis is major source for
holds 4 patents. Professor Tsang has received numerous awards and honours, fulfilling high energy demand of cancer, but the role of metabolic reprogramming and its
including the Award of Excellence in Reproductive Medicine, the highest award regulatory mechanism in OVCA cells remain unknown. p53 is key tumor suppressor involved
offered by the Canadian Fertility and Andrology Society. He has developed strong in apoptosis and frequent defect of p53 exist in epithelial OVCA. Hexokinase II (HKII) is key
academic and research partnerships in China, Taiwan, Japan and South Korea. metabolic enzyme involved in the first step of glycolysis and its frequent presence in the
mitochondria have been reported in multiple cancers. We demonstrate here that CDDP-induced,
p53-mediated HKII down-regulation and mitochondrial p53-HKII interaction are determinants
of chemosensitivity in OVCA. CDDP decreased HKII expression, altered its cellular
localization and glycolysis in p53-wt chemosensitive OVCA cells, two responses lost with p53
deficiency. Our data suggest that CDDP-activated p53 interacts with HKII and facilitates its
entry into the nucleus. Upon entry to the nucleus, P-p53 (Ser15) transcriptionally regulates
HKII, contributing to down-regulation of HKII and aerobic glycolysis and eliciting apoptosis
in chemosensitive OVCA cells. Conversely, this response is compromised in p53 defect
chemoresistant cells. The nuclear HKII-P-p53 intracellular trafficking is associated with
chemosensitivity in vitro and in vivo, suggesting nuclear HKII-P-p53 interaction may function
as a prognostic biomarker for chemosensitivity in epithelial OVCA. Our findings highlight the
involvement of P-p53 in the regulation of HKII intracellular trafficking and expression in
controlling glycolysis and chemo-responsiveness in OVCA.

Keywords:
ovarian cancer, chemoresistance, p53, hexokinase II, glycolysis, apoptosis

O. 5-5 Isolation and Analysis of Extracellular Vesicles as Circulating Biomarkers
for Prostate Cancer Diagnosis
Dr. Yong Li, currently working as an Associate Professor,
Faculty of Medicine, UNSW Sydney, Australia as well as Principal Scientific Duojia Wu1,2, Ying Zhu1,2, Jie Ni1,2, Bairen Pang1,2, Julia Beretov1,2, Joseph Bucci1,2, David
Officer and Director of Cancer Research Lab, Cancer Care Centre, St George Malouf1,2, Peter Graham1,2, Yong Li,1,2*
Hospital, Australia. Dr. Li, obtained his PhD degree at University of New South
Wales (UNSW), Australia in 2000 and then joined as Post-Doctoral Fellow at the 1Cancer Care Centre, St George Hospital, Kogarah, NSW 2217, Australia 2St George and
Cancer Care Centre, St George Hospital, UNSW and eventually become Sutherland Clinical School, UNSW Australia, Sydney, NSW 2052, Australia.
Associate Professor and Full Professor (U. He became expertise in cancer
biomarker discovery, radiation biology, target cancer therapy and cancer ABSTRACT
metastasis. He has published more than 100 papers and book chapters in peer-
reviewed journals in cancer research area. His current research program is aimed Prostate cancer (CaP) is a major health problem in western countries. Extracellular vesicles
at a): To investigate novel biomarkers from human body fluids and tissues, cancer (EVs) contain pathogenic components, such as proteins, DNA fragments, mRNA, non-coding
cell lines and animal models for cancer diagnosis and developing personalized RNA and lipids, and play a critical role in intercellular communication. EVs hold promise for
medicine; b): To investigate mechanisms of cancer metastasis and chemo-/radio- the discovery of liquid biopsy-based biomarkers for CaP diagnosis. The objective of this study
resistance and role of tumour microenvironment, cancer stem cells and epithelial is to discover biomarkers from cancer-derived EVs in CaP cell lines, blood and urine using a
mesenchymal transition in cancer progression; c): To use targeted cancer therapy number of new techniques for CaP diagnosis. EVs were isolated from precipitation reagent,
and combination therapy to control metastatic and therapeutic resistant cancers. ultracentrifugation (UC), polymer precipitation (PP) size exclusive chromatography (SEC),
Dr. Li, was awarded the best Medical Research Award-NHMRC Achievement and confirmed by Cryoelectron microscopy (Cryo-EM) for morphology and nanoparticle
Award in 2010. He currently serves on the Associate Editor of World Journal of tracking analysis (NTA) for size distribution and concentration. High-throughput LC-MS/MS-
Cancer Research (USA), and on the editorial board of the Open Biomarker based label-free quantification was performed to reveal protein profiling of urinary exosomes
Journal (USA). in CaP patients and normal controls, followed by systematic comparison of differentially
expressed proteins between the groups. We studied EVs by a series of isolation and analysis
techniques. These preliminary results are promising and are the technique fundamentals for our
ongoing research by using EVs for liquid biopsy-based CaP early detection. Our findings also
demonstrate that urinary exosomes harbour informative proteins that might be used for the
detection of CaP or monitoring its progression through a non-invasive way.

Keywords:
prostate cancer, extracellular vesicles, exosomes, diagnosis

O. 6-1 Food-derived and endogenous collagen peptide, Pro-Hyp, specifically
stimulates growth of fibroblast with somatic stem cell marker, p75NTR
Dr. Kenji Sato, is a Professor of Division of Applied Biosciences, Graduate
School of Agriculture, Kyoto University. He graduated from Kyoto University Kenji Sato
and worked as an Assistant Professor, Associate Professor, and Professors of
Kyoto Prefectural University. His research has focused on metabolic fate and Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University,
biological activities of peptides in food. He has identified food-derived peptides Kitashirakawa Oiwake-cho, Kyoto 606 8502, Japan
in human and animal bodies and elucidated their biological activities, which can
be associated to the beneficial effects by ingestion of peptides in food protein ABSTRACT
hydrolysate and fermented food. He also has developed methods for large scale
peptide fractionator and identified active peptides by ingestion by using this It has been demonstrated that oral administration of collagen hydrolysate enhances
apparatus. He has published over 120 papers in international journals and 20 book healing of pressure ulcer. Now, collagen hydrolysate is listed in the guideline for
chapters. His outstanding contributions to food science have been recognised by therapy of pressure ulcer. We have demonstrated that Pro-Hyp is present in human
Achievement Award for Young Scientists in Fisheries Science (1995) by The blood after ingestion of collagen hydrolysate and also generated by degradation of
Japanese Scoety of Fisheries Science, Technology Award (2014) by The Japanese endogenous collagen in the wound-healing site. The Pro-Hyp enhances growth of
Society for Food Science and Technology, and Science Merit Award (2016) by mice fibroblasts attached on collagen gel, which has been associated to
International Society of Neutraceuticals and Functional Foods. He is serving enhancement of wound healing. However, excess growth of fibroblasts in normal
associate editor of npj Science of Food and editorial boards of Food Science and tissues by Pro-Hyp has not been reported, which suggests that specific fibroblasts
Technology Research and Journal of Food Bioactives. respond Pro-Hyp in body. Most of fibroblasts migrated from mice skin for first few
days had somatic stem cell maker, p75NTR. The p75NTR-positive fibroblasts,
however, decreased to less than 1% and almost 0% after 2 and 4 weeks cultivation,
respectively. Pro-Hyp increased the fibroblast (2 weeks) on plastic and collagen
gel but did not the fibroblast (4 weeks). The percentage of p75NTR-positive cells
(2 weeks) was significantly increased by addition of Pro-Hyp. These facts indicate
that Pro-Hyp can enhance growth of fibroblast with p75NTR, possibly just
differentiated from somatic stem cell in wound healing site, but does not fibroblasts
without expressing p75NTR in normal tissues.

Keywords:
Collagen peptide, Pro-Hyp, somatic stem cell, fibroblast, p75NTR, wound healing.

O. 6-2 Microbiota-Gut-Brain Axis, Psychobiotics, and Neuropsychiatric disorders

Dr. Ying-Chieh Tsai, is a distinguished professor at the Institute of Biochemistry Ying-Chieh Tsai
and Molecular Biology at National Yang-Ming University, Taipei, Taiwan. Dr.
Tsai, is also a Director of Microbiome Research Center. He received his Ph.D. in Institute of Biochemistry and Molecular Biology,
Microbiology from Tokyo University. Dr. Tsai, founded the Taiwan Association National Yang-Ming University, Taiwan
of Lactic Acid Bacteria where he served as President during until 2006. He served
concurrently as Vice-President of the Asian Federation of Societies for Lactic ABSTRACT
Acid Bacteria, after which he served as President until 2009. In addition to his
academic achievements, Dr. Tsai actively pursues several commercial ventures, Psychobiotics, a class of probiotics with psychotropic activities, integrates neural,
including Yangsen Biotechnology Co. Ltd. which he founded in 2002, Kei-Sei- hormonal, and immunological signaling via the gut-brain axis is proved for
Ken Biotechnology Co, Ltd. two years later, and most recently, Bened combating a broad spectrum of complex diseases including mental illness,
Biomedical Co. Ltd. in 2015. Currently, Professor Tsai specializes in irritable bowel syndrome (IBS), neurodegenerative disorders. Lactobacillus
psychobiotics and conducts ground-breaking research on the microbiota-gut brain plantarum PS128 (PS128) is a novel psychobiotic, which normalized depression-
axis where he explores its commercial and health benefits. He has published more like behaviours in early life-stressed mice and improved locomotion in
than 100 scientific articles in various International and National Journals Parkinson’s disease in mice. Moreover, PS128 reduced the 5-HTP-induced
including many book chapters. He also guided/supervised many master and visceral hypersensitivity in a rat model of IBS and reversed the 5-HT(2A/2C)
doctorate scholars during his academic career. agonist 1-(2, 5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) induced
Tourette syndrome-like responses in rats. In a 4-week, randomized, double-blind,
parallel, placebo-controlled study investigated the effects of PS128 on boys (aged
10.0 ± 2.31) with autism spectrum disorder (ASD) (n = 72), PS128 appeared to
reduce scores for body and object use, hyperactivity, opposition, anxiety,
problems related to thoughts, and rule-breaking behaviors, whereas the placebo
exerted insignificant effects. Based on an analysis stratified by age, younger
children (7-12 years) experienced greater benefits than older children (13-15
years), conveying the importance of early interventions. These results of
behavioral tests in rodents and ASD clinical study showed that PS128 is a feasible
and natural intervention for alleviating symptoms of neuropsychiatric disorders.

Keywords:

Psychobiotics, gut-brain axis, Lactobacillus plantarum (PS128), ASD

O. 6-3 Use of Common Foods for Health Benefits in Developed and

Dr. Perry K.W. Ng, is a Professor and Associate Chair at Department of Food Developing Countries
Science and Human Nutrition, Michigan State University (MSU), USA. Dr. Ng,
graduated (doctorate) from University of Manitoba, Canada and joined as a Perry K.W. Ng
graduate teaching assistant and research associate. Then he moved to MSU, USA
and positioned as Assistant, Associate Professor and eventually and become Department of Food Science and Human Nutrition, Michigan State University, East
Professor (since 1992 to till date). His research foci have been in the areas of Lansing, Michigan, USA
physicochemical and genetic properties of wheats in relation to end-use qualities,
cereal chemistry and technology, and extrusion technology in cereal processing, ABSTRACT
and in recent years have extended into modifying certain components (e.g.,
resistant starch, fiber, phenolics) in cereals and legumes with potential for A number of chronic diseases and health disorders, e.g., diabetes and obesity,
functional food ingredients. Professor Ng was named Fellow of the American are generally more prevalent in developed countries and now are also emerging
Association of Cereal Chemists International in 2010 and Fellow of the challenges in developing countries. In recent years, “food as medicine” and
International Association for Cereal Science and Technology Academy in 2012. “food for health” have become popular topics for discussion. How can certain
He is in the Editorial Board member of various popular international Journals common foods be of health benefit to humans and what are the mechanisms of
including Cereal Chemistry, The Crop Journal and The Food Science and Human these benefits? Abundant research studies reported in the literature have
Wellness Journal. Dr. Ng, has published more than 140 scientific papers with 2 provided evidence that regular consumption of high-fiber-content foods, e.g.,
books chapters and possess two US patents as well. dry beans and whole grains, could improve human health by reducing the
incidence of health disorders such as hyperglycemia, hyperinsulinemia, obesity,
cardiovascular diseases, and cancers. The food industry has been increasing its
demand for plant-based ingredients with diverse functionalities, for the
development of high-quality, nutritious and healthy food products. Research has
focused on utilizing extrusion, fermentation, hydrothermal treatment, high-
pressure treatment, germination, and enzymatic modification to modify food
ingredients from different plant materials (e.g., cereals and pulses). Targeted
functional properties include thermal and pasting properties, water holding
capacity, oil binding capacity, protein solubility, emulsifying ability, foaming
capacity, gel formation, and starch digestion rate. In this presentation, some of
these research foci will be discussed. Selected studies conducted in our
laboratory to prepare value-added food ingredients from cereals and pulses, with
anticipation of using these foods for health benefits, will be presented.

Keywords:

Food, health, cereals, pulses, food properties, benefits.

O. 6-4 TEMPE: NUTRITIOUS AND HEALTHY FOOD MADE FROM
SOYBEAN FERMENTATION
Dr. Giyatmi Irianto has completed her Master (1998), and Doctor (2005) at the
Bogor Agricultural Institute in Food Science and Technology, Indonesia. She Giyatmi Irianto
currently working as a lecturer at Food Technology Studies Program of Sahid
University Jakarta since 1994. Structural positions ever held are Head of Food Food Technology Department, Sahid University of Jakarta – INDONESIA
Technology Department (1998-1999), Dean of Faculty of Agriculture Industry
Technology (1999 - 2007), Vice Rector of Academic Affairs (2007-2014) and ABSTRACT
Director of Post Graduate School (2014-2015), since 2016 until now as Head of
Institute of Research and Community Service of the University of Sahid Jakarta. Tempe is a fermented food made from soybean originally produced by Indonesian.
Dr. Giyatmi Irianto is also active as a Chairman of the Division of Research and The fermentation process uses several types of Rhizopus fungi, such as Rhizopus
Community Service, in Association of Indonesian Lecturers DKI Jaya Region oligosporus, Rh. oryzae, Rh. stolonifer, or Rh. arrhizus. Ecological studies
(2006-2011), Vice Chairman of the Indonesian Association of Food Technology showed that bacteria and yeasts also play significant roles in tempe production.
(2012-2018). Head of Research Division of Private Higher Education Association Tempe is recognized by Indonesian Government as a National Intangible Cultural
of DKI Jakarta Area (2012 – present). She also guided several master and Heritage and has been recognized as one of the world’s superfood. Potential
undergraduate students and published several papers in various peer-reviewed market of tempe is not only in domestic, but also international scale. Proudness
Journals. She also attended numerous international and national conferences and of Indonesia as contributor for popular healthy food potential to substitute animal
shared her research results and ideas. protein appreciate globally. Process of making tempe consist of several stages,
namely boiling, soaking, peeling, addition of inoculum, wrapping, and
fermentation for 18 – 36 hours at 20 - 37⁰C. The fungi grown in soybeans
hydrolyzes complex compounds of protein into simple compounds that are easily
digested by humans. Privileges of tempe are source of protein with completely
essential amino acids, rich in dietary fiber, high in vitamins, minerals, low in fat,
sodium & inhibitors, cholesterol free, and also probiotic source. Other benefits of
consuming tempe as disease prevention, such as anemia, antidiarrhea,
antibacterial, hypertension, cancer, antiinfection, reduce cholesterol, coronary
heart disease, and osteoporotic.

Keywords:
Complete nutrition, Indonesian fermented food, Superfood, Soybean

fermentation.

O. 6-5 Anti-aging effects and mechanisms of traditional Taiwanese botanic
extracts
Dr. Chia-Jui Weng, is a Professor at the Department of Living Services Industry,
Tainan University of Technology, Tainan, Taiwan. Currently he is a Vice- Shwu-Ling Peng, Chiung-Man Tsai, Shun-Fa Yang*, Chia-Jui Weng*
President of Tainan University of Technology, Taiwan. Dr. Chia-Jui Weng Shwu-Ling Peng: Institute of Medicine. Chung Shan Medical University, Taichung, Taiwan.
research areas focus on Hepatocarcinoma, Natural bioactives and phytochemicals, Chiung-Man Tsai: Tainan Hospital, Ministry of Health and Welfare, Tainan City, Taiwan
cancer invasion and metastasis, single nucleotide polymorphism, anti-aging. He Shun-Fa Yang*: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan
is as a Lead Guest Editor of BioMed Research International, Guest Editor of Chia-Jui Weng*: Department of Living Services Industry,Tainan University of Technology,
Evidence-Based Complementary and Alternative Medicine (eCAM) Journal,
Editorial Board Member of Journal of Turgut Ozal Medical Center (ISSN: 1300- Tainan City, Taiwan
1744), Editorial Board Member of Journal of Tumor (ISSN 1819-6187),
Honorary Editorial Board of Journal of Hepatocellular Carcinoma (ISSN: 2253- ABSTRACT
5969), Professional reviewer of technical research paper abstract of 2012 IFT
Annual Meeting and Professional reviewer of ACS publications. He has been Chronic micro-inflammation is a hallmark of many aging-related neurodegenerative and
honours and Awards with Academic Award of Taiwan Association for Food metabolic syndrome-driven diseases. In high glucose (HG) environment, reactive oxygen species
Science and Technology and Academic Award of Health Food Society of Taiwan. (ROS) is generated and the ROS induced inflammation, cytokines secretion, DNA damage, and
He has published more than 50 research papers in renowned International and cell cycle arrest to lead to cellular senescence. Water chestnut shell (WCS) is a plant hull which
National Journals. containing polyphenolic compounds and showed antioxidant and anticancer activities. Orchid,
which containing a natural polysaccharide compound, possesses many physiological activities
including anti-inflammatory and neuroprotective effects. These agricultural plants might be able
to reduce oxidative stress and inflammation. This study was used HG-induced human normal
dermal fibroblasts (HG-HNDFs) as an in vitro model to disclose the effects of water extract of
Phalaenopsis orchid flower (WEPF) and ethanol extract of water chestnut shell (EEWCS) on the
anti-aging and their underlying molecular mechanisms. The toxicity of extracts on human normal
dermal fibroblasts (HNDFs) was determined by MTT method. The senescence of cells was
assayed by β-galactosidase (SA-β-gal) kit. ROS and nitrate production was analyzed by
Intracellular ROS contents and ELISA, respectively. Western blotting was used to detect the
proteins in cells. The results showed that the exposure of HNDFs to HG (30 mM) for 72 h were
caused cellular senescence and arrested cells at G0/G1 phase. Indeed, the treatment of HG-
HNDFs with WEPF (200 g/ml) and EEWCS (10 g/ml) significantly released cell cycle arrest
and promoted cell proliferation. The G1/S phase transition regulatory proteins such as protein
retinoblastoma (pRb), p53, and p16INK4a depressed by WEPF and EEWCS were also observed.
Additionally, the treatment of WEPF and EEWCS increased the activity of HO-1 through
upregulating Nrf2 as well as decreased the ROS and NO of HG-HNDFs. Therefore, the
senescence marker protein-30 (SMP30) in cells was diminished. In conclusion, the WEPF and
EEWCS might inhibit HG-induced aging of HNDFs by reducing oxidative stress and free
radicals.

Keywords:
Taiwanese botanic extract, anti-aging, high glucose, Phalaenopsis orchid flower, water
chestnut shell.

O. 6-6 Improvement of vinegar drink on sport

Dr. Kamesh Venakatakrishnan is a Post-Doctoral Fellow (PDF) in School of Kamesh Venkatakrishnan, Chin-Kun Wang
Nutrition, Chung Shan Medical University, Taiwan. He received his masters and
doctorate degree in Medical Biochemistry from University of Madras, Tamil School of Nutrition, Chung Shan Medical Hospital, Taiwan
Nadu, India. Before he arrives to Taiwan he worked as a Lab Incharge
(Biochemist) at Chidambaram Medical Collage Hospital for 6 months. His field ABSTRACT
of research is focused on identification of novel nutraceuticals/functional foods
from traditional food and modern food materials as well as isolation of various Nutrition plays a crucial role in athlete health, fitness and training ability and thus
active phytocomponents from traditional herbs and explore its various biological inflict a direct impact on athlete performance. Ample amount of studies (pre-
properties in both human and animal model (pre-clinical and clinical studies) clinical and clinical) have indicated that various functional foods/nutraceuticals
especially against metabolic syndrome like hyperglycemia, hyperlipdemia, anti- (nutritional supplements) could enhance the athletic performance in endurance
obesity. Dr. Kamesh, is also the active member of International Society for athletes by lowering muscle weakness (fatigue), oxygen consumption, increase
Nutraceuticals and Functional Foods (ISNFF) and Indian Society of mitochondrial biogenesis as well as abolish exercise-induced fatigue or oxidative
Atherosclerosis Research (ISAR). He also joined in several National and stress. The purpose of this cross-over design clinical trial is to compare the
International conferences/congress as a speaker and audience. In addition, he athletic performance or endurance capacity in healthy sports person after
published many research articles in peer-reviewed international and national consumption of vinegar drink (experimental) and commercial sports drink (Shu
Journals. Dr. Kamesh serves as a reviewer for various International Journals like Run Sports drink-Placebo/standard). Seven healthy individuals are chosen and
Functional Foods, Pharmaceutical Biology, Journal of Food Biochemistry, asked to drink vinegar drink and commercial sports drink and the levels of blood
Experimental and Therapeutic medicine. glucose, lactose, Ammonia, non-esterified fatty acids (NEFA), respiratory
exchange ratio (RER by ergometer) were determined. The outcome of this study
clearly shows that no significant difference in athletic performance or endurance
capacity after the consumption of both vinegar drink and commercial sports drink.
Hence, indicate that vinegar drink (functional food) with BCAA (Branched chain
amino acids) like Leucine, iso-leucine and valine along with vinegar could act as
a good ergogenic agent and used as a natural sports drink.

Keywords:

Vinegar, functional food, endurance, BCAA, ergogenic.

O. 7-1 A mesothelioma cancer stem cell survival factor

Dr. Richard L. Eckert, is a Distinguished Professor and Chair at University of Eckert, RL
Maryland School of Medicine. Dr. Eckert, received his PhD at the University of Department of Biochemistry and Molecular Biology, University of Maryland School of
Illinois, Urbana, and completed post-doctoral research training at Harvard Medicine, Baltimore, MD, USA, Department of Dermatology, University of Maryland School
Medical School. He was a Professor at Case Reserve University School of of Medicine, Baltimore, MD, USA, Department of Reproductive Biology, University of
Medicine until 2006 when he joined the University of Maryland, School of
Medicine as Professor and Chair of the Department of Biochemistry and Maryland School of Medicine, Baltimore, MD, USA
Molecular Biology. Dr. Eckert is an internationally recognized investigator who
has made pioneering discoveries in the area of surface epithelial biology – ABSTRACT
specifically focusing on the human epidermis. His studies have led to enhanced
understanding of normal skin biology and to insights regarding the mechanisms Mesothelioma is an aggressive treatment resistant and fatal disease that is
that drive skin diseases including cancer and psoriasis. He also has a long-term initiated by exposure to asbestos. Surgical reduction and chemotherapy (cisplatin
interest in the prevention of skin cancer by natural biological agents. Dr. Eckert and pemetrexed) are first line treatments, but recurrence of highly aggressive and
has published 200+ journal articles and reviews, and his trainees have presented drug-resistance disease is common. We show that tissue transglutaminase (TG2),
as many meeting abstracts. He serves as an editorial board member and reviewer a GTP binding cancer stem cell survival and drug-resistance regulator, is highly
for a host of cancer journals, and has served on numerous grant review panels and enriched in human mesothelioma tumors and in mesothelioma cancer stem cells
society boards. He is a University of Wisconsin Distinguished Alumni (MCS cells). In addition, TG2 knockdown or TG2 inhibitor treatment, reduces
Achievement Award recipient, an elected member of the Board of the Society for MCS cell survival, spheroid formation, matrigel invasion, migration and tumor
Investigative Dermatology, a standing member of the review panel of the formation. These are important observations as, MCS cells cells comprise a
American Institute for Cancer Research. Dr. Eckert holds patents from the United highly aggressive subpopulation of tumor that form rapidly growing and
States Patent Office, and has been continuously funded by the National Institutes aggressive tumors. In addition, TG2 loss is associated with reduced levels of
of Health as a principal investigator since 1989. mRNA encoding a wide range of cancer stem cell and epithelial-mesenchymal
transition proteins. These studies indicate that TG2 is an important MCS cell
survival protein that drives expression of cancer stem cell and EMT markers to
enhance MCS cell survival and invasion, and suggest that TG2 is an important
mesothelioma cancer stem cell therapy target.

Keywords:

cancer stem cells, cancer prevention

O. 7-2 A novel binding protein regulating dual functions of cathepsin B in triple-
negative breast cancer cells
Dr. Seong-Jin Kim is a Director of Precision Medicine Research Center at the
Advanced Institutes of Convergence Technology of Seoul National University. Seong-Jin Kim
He also serves as a Visiting Professor of the Ireland Cancer Center at Case
Western Reserve University, USA, and as a Visiting Professor at Tsukuba Precision Medicine Research Center, Advanced Institutes of Convergence Technology,
University, Japan. He received his doctorate from Tsukuba University, Tsukuba, Seoul National University, C-504, AICT, 145 Gwanggyo-ro, Yeongtong-gu, Suwon-si,
Japan.He served as a tenured senior investigator in the Laboratory of Cell
Regulation and Carcinogenesis at the National Cancer Institute in Bethesda, Gyeonggi-do, 16229, Republic of Korea
Maryland, USA. He returned to Korea as the first president of the Lee Gil Ya
Cancer and Diabetes Research Institute of the Gachon University of Medicine ABSTRACT
and Science in 2007. Dr. Kim also was the President of the CHA Research
Institutes for Bio-Medical Sciences and the Director of the CHA Cancer Institute The development of triple-negative breast cancer (TNBC) negatively impacts
from September 2010 to June 2016. His field of Interest are Tumor Biology. Dr. both quality of life and survival in a high percentage of patients. Therefore, the
Kim has published more than 240 peer-reviewed articles and over 200 abstracts discovery of novel biomarkers that may facilitate the development of more
and book chapters in his career. Dr. Kim is one of the Personal Genome Pioneers effective, molecularly targeted therapies remains a high priority. Here, we report
in the world. He became the fifth individual in history, and the first Korean, to that Bcl-2-associated athanogene 2 (BAG2) is significantly overexpressed in
have his DNA blueprint decoded. He led the Korean Genome Sequencing project TNBC and is strongly associated with poor clinical outcomes. Interestingly,
while he was the Director at the Lee Gil Ya Cancer and Diabetes Institute, South BAG2 blocks the proteolytic processing of pro-cathepsin B via direct interaction
Korea in 2007. Dr. Kim has received many outstanding awards such as HoAm with the pro-peptide region, leading to the secretion of pro-cathepsin B that
Prize in Medicine. induces metastasis, whereas silencing BAG2 induces apoptosis by increasing the
mature form of cathepsin B and suppresses lung metastasis. Collectively, our
results uncover BAG2 as a regulator of the oncogenic function of cathepsin B and
a potential diagnostic and therapeutic target that may reduce the burden of
metastatic breast cancer.

Keywords:
triple-negative breast cancer, biomarkers, BAG2, cathepsin B

O. 7-3 Pancreatic DCLK1+ cells originate distinctly from PDX1+ progenitors and
contribute to the initiation of intraductal papillary mucinous
Dr. Gloria H. Su, is a Professor at the Departments of Pathology and Cell neoplasm in mice
Biology and Otolaryngology/Head & Neck Surgery at Columbia University
Medical Center (CUMC). Dr. Su’s laboratory has developed multiple genetically- Wanglong Qiu a, b, Helen E. Remotti a, Sophia M. Tang b, Elizabeth Wang b, Lily
engineered mouse models that recapitulate human pancreatic cancer at both Dobberteen b, Ayman Youssof a, b, Joo Hee Lee b, Edwin C. Cheung b, Gloria H. Su a, b*
genetic and histologic levels. Using these genetically-engineered mouse models,
Dr. Su’s team is interrogating the biology of tumor development, progression, aThe Department of Pathology & Cell Biology, Columbia University Medical Center, New
and metastasis. Notably, her team has reported that the loss of the wild-type KRAS York, NY 10032, USA. bHerbert Irving Comprehensive Cancer Center, Columbia University
is associated with pancreatic cancer metastasis in mice and in humans. They have Medical Center, New York, NY 10032, USA. cDepartment of Otolaryngology and Head and
also demonstrated that the inactivation of different tumor-suppressor genes
following Kras activation may influence the dichotomy of PanIN and IPMN Neck Surgery, Columbia University Medical Center, New York, NY 10032, USA
(precancerous lesions of pancreatic ductal adenocarcinoma) development and
progression. Specifically, the inactivation of the activin signaling preferentially ABSTRACT
promotes the development of IPMN. In addition to mouse model, Dr. Su and her
team have contributed to our understanding of the cancer genetics of human PanINs and IPMNs are the two most common precursor lesions that can progress
IPMN and recently shown that the dysregulation of the PI3K-PTEN signaling to invasive pancreatic ductal adenocarcinoma (PDA). DCLK1 has been identified
pathway is associated with poor prognosis among IPMN patients. Dr. Su as a biomarker of progenitor cells in PDA progressed from PanINs. To explore
currently serves on the editorial boards of Scientific Reports, Cancer Letters, and the potential role of DCLK1-expressing cells in the genesis of IPMNs, we
Genes & Diseases. She also serves on the study sections for the NIH, DOD, compared the incidence of DCLK1-positive cells in pancreatic tissue samples
AACR, etc. from genetically engineered mouse models (GEMMs) for IPMNs, PanINs, and
acinar to ductal metaplasia by immunohistochemistry and immunofluorescence.
Mouse lineage tracing experiments in the IPMN GEMM showed that DCLK1+
cells originated from a cell lineage distinct from PDX1+ progenitors. The
DCLK1+ cells shared the features of tuft cells but were devoid of IPMN tumor
biomarkers. The DCLK1+ cells were detected in the earliest proliferative acinar
clusters prior to the formation of metaplastic ductal cells, and were enriched in
the “IPMN niches”. In summary, DCLK1 labels a unique pancreatic cellular
lineage in the IPMN GEMM. The clustering of DCLK1+ cells is an early event
in Kras-induced pancreatic tumorigenesis and may contribute to IPMN initiation.

Keywords:
Pancreatic ductal adenocarcinoma, PanIN, IPMN, DCLK1, tuft cells.

O. 7-4 Inositol Hexaphosphate and Prostate Cancer Chemoprevention: Efficacy
and Mechanism
Dr. Rajesh Agarwal, is a Professor and Vice Chairman, Department of
Pharmaceutical Sciences, University of Colorado Denver (UCD), CO, USA. Dr. Rajesh Agarwal 1,*, Komal Raina1, Chapla Agarwal1
Rajesh Agarwal, graduated from the Lucknow University, India in 1981 with
Ph.D. degree. He became Assistant Professor in 1985 at Manipur University and 1School of Pharmacy, Department of Pharmaceutical Sciences, University of Colorado
moved to Case Western Reserve University (CWRU), Ohio, USA and worked as Denver, 12850 E. Montview Blvd, C238, Aurora, CO 80045, USA. Phone: 1-303-724-4055
Research Associate and Assistant Professor. Later, Joined UCD and eventually
become Professor. His field of research interest lies on the area of diet, nutrition ABSTRACT
and disease prevention and intervention, with specific training and expertise in
establishing molecular mechanisms and efficacy of a series of natural and Prostate cancer (PCA) is the most frequently diagnosed malignancy and second
synthetic agents as cancer chemopreventive and anti-cancer agents. In the past 30 leading cause of cancer-associated deaths in males in the United States. PCA
years, his team members as identified and established a series of natural products growth and progression involve aberrant cell cycle progression including a
for their efficacy and associated mechanisms against various epithelial cancers decrease/loss of cyclin-dependent kinase inhibitor (CDKI) function, suggesting
including prostate cancer. He is an editorial board member of several lead cancer induction in CDKI level/function could be a promising approach to manage PCA.
journals including Cancer Research, Cancer Prevention Research, PLOS ONE, Inositol hexaphosphate (IP6) is present in most cereals, legumes, nuts, oil seeds
Molecular Cancer Therapeutics. He is a Recipient of 2006 Shell Award for and soybean, and taken orally as an over-the-counter dietary/nutrient supplement
outstanding achievements in research and graduate education, School of for its several health benefits. In PCA cell culture, IP6 up-regulated CDKI
Pharmacy, University of Colorado Denver and received Outstanding Kip1/p27 and Cip1/p21 levels, eventually causing a G1 arrest, cell growth
Achievement Award, Society of American Asian Scientists in Cancer Research, inhibition and apoptosis. Next, employing genetic approaches, we found that p27
2009. He has over 370 peer-reviewed publications and several book chapters, has and p21 are required for IP6 efficacy against PCA in both cell culture and mouse
been an invited speaker across the globe, has over three hundred presentations in xenograft models. Efficacy studies in genetic mouse model also found that IP6
national and international scientific meetings. strongly suppresses growth and progression of PCA by altering tumor vascularity
and the energy generating metabolic events in the tumor cells, which are essential
for tumor sustenance. Together, these findings suggest IP6 potential in
suppressing growth and progression of PCA in humans, and advocate for a
potential clinical trial of IP6 in PCA patients, which may improve their morbidity
and survival time.

Keywords: Prostate Cancer; Inositol hexaphosphate; Chemoprevention; Cell
Cycle Regulators.

O. 7-5 Targeting Metabolism for Cancer Prevention

Dr. Shivendra V. Singh, currently an Associate Director for Basic research and Krishna B. Singh and Shivendra V. Singh*
UPMC Chair in Cancer Prevention Research at University of Pittsburgh, USA.
He earned his M.Sc. and Ph.D. degrees in Biochemistry from the Banaras Hindu Department of Pharmacology & Chemical Biology, and UPMC Hillman Cancer Center,
University (India). In 1983, Dr. Singh came to the United States to pursue post- University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
doctoral training at the University of Texas Medical Branch, Galveston, TX and
studied glutathione-linked detoxification mechanisms. In 1988, Dr. Singh was ABSTRACT
appointed to the faculty of the University of Miami, School of Medicine as an
Assistant Professor in the Department of Medical Oncology. The Mercy Hospital Prostate cancer is a leading cause of cancer-related mortality especially in western
of Pittsburgh recruited Dr. Singh in 1992 to head its Cancer Research Laboratory countries. Chemoprevention using safe and inexpensive phytochemicals from
and direct basic cancer research program. Dr. Singh joined the University of edible or medicinal plants is clinically attractive for reducing both morbidity and
Pittsburgh in 2000 as a Tenured Professor of Pharmacology & Chemical Biology mortality from prostate cancer. Increased de novo synthesis of fatty acids
and member of University of Pittsburgh Cancer Institute (now known as UPMC (lipogenesis) is a rather unique and targetable mechanism of human prostate
Hillman Cancer Center). Dr. Singh holds a secondary appointment in the cancer. Moreover, β-oxidation of fatty acids is the principal bioenergetics
Department of Urology in the School of Medicine, and is the Associate Director pathway in human prostate cancer as opposed to other epithelial solid tumors that
for Basic Research at the UPMC Hillman Cancer Center. Dr. Singh was honored rely on glycolysis (Warburg effect) to meet cellular energy demands. A role for
with UPMC Endowed Chair in Cancer Prevention Research in 2014. Dr. Singh is increased de novo synthesis of fatty acids in pathogenesis of prostate cancer is
a co-author of over 261 research articles in peer-reviewed journals and 27 review substantiated by studies showing overexpression of mRNA/protein levels of key
articles/book chapters. Dr. Singh has served on the Editorial Boards of various fatty acid synthesis enzymes, including ATP citrate lyase, acetyl-CoA
prestigious cancer-related journals, including Clinical Cancer Research, Cancer carboxylase 1, and/or fatty acid synthase in early (prostatic intraepithelial
Prevention Research, Molecular Cancer Therapeutics, Molecular Pharmacology neoplasia) and/or late-stage (adenocarcinoma) prostate cancer when compared to
and so on. normal tissue. Unfortunately, a clinically-acceptable (non-toxic) inhibitor for
fatty acid acid synthesis is still lacking. In this presentation, we provide
experimental evidence for in vitro and in vivo inhibition of lipogenesis by
isothiocyanate class of chemopreventive agents that are safe and present in
cruciferous vegetables. This investigation was supported by the USPHS/NCI
grant CA101753-14.

Keywords:
Lipogenesis, Glycolysis, Prostate Cancer, Prevention

O. 8-1 Industrial hemp: an excellent source of functional ingredients for
nutrition and health
Dr. Petras Rimantas Venskutonis, is a Professor and leader of research team
‘Functional Food Materials’ at the Department of Food Science and Technology Petras Rimantas Venskutonis
of Kaunas University of Technology, Kaunas, Lithuania. He obtained his PhD
from National University of Food Technologies in Kiev, Ukraine. His major Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų
research focus has been in the area of development of new natural functional pl. 19, Kaunas, Lithuania
ingredients for foods and nutraceuticals from various plant origin materials.
Currently his research group involved in developing a biorefining technologies ABSTRACT
for the separation of valuable substances from agro-food raw materials,
processing by-products and waste using multistep supercritical fluid, pressurized Industrial hemp is one of the fastest growing plants: it belongs to the same Cannabis sativa
liquid, enzyme assisted, chromatographic and other extraction/fractionation species, which has been used as a psychoactive tetrahydrocannabinol (THC) containing drug.
methods. He is an author and co-author of over 300 publications in peer-reviewed Its content in industrial hemp is remarkably lower, whereas other phytocannabinoids such as
journals and 8 book chapters. His articles were cited almost 5000 times, Hirsh cannabidiol (CBD) and cannabigerol (CBG) may be present at high concentrations.
citation index hi=34. He has been national delegate in various international Cannabichromene (CBC), cannabidivarin (CBDV) and tetrahydrocannabivarin (THCV) are
scientific organizations and EU research programs; he is a member of several other cannabinoids tested for their bioactivities.
international professional organizations, incl. Institute of Food Technologists
(IFT) and International Society of Nutraceuticals and Functional Foods (ISNFF). Table 1. Health benefits and bioactivities of selected phytocannabinoids
Lithuanian National Science Award winner in 2004. He is an Editorial Board
member of various international Journals like American Journal of Advanced CBD
Food Science and Technology, Journal of Food-CyTA, Journal of Bioactive
Compounds and so on. Anxiolytic[CB1(+); 5-HT1A(+)] Antipsychotic Antiepileptic Neuroprotective
[TRPV1 (+)] [Ca2+] [Ca2+]; ROS

Vasorelaxant Analgesic Anti-ischemic Antiemetic
[PPAR (+) [TRPV1 (+)] [5-HT1A(+)]

Antiproliferative/Anticancer Intestinal anti- Anti-inflamatory Immunosuppresive

[Ca2+]; ROS; CB2(+); Id-1] prokinetic [TNF-; Adenosine [T -cellss]
[Ca1(-); FAAH] uptake]

Antipsoriatic Antibacterial Antispasmodic Bone-stimulant Antidiabetic

CBC

Antimicrobial Bone-sti m u la nt Analgesic Antiproliferative

CBDV CBG

Bone-stimulant Bone-sti m u la nt Antibacterial Antiproliferative

9-THCA 9-THCV

Antiproliferative Antispasmodic Anorectic Bone-sti m u la nt Antiepileptic

[CB1(-)] [CB2(+)] [CB 1(-);G AB A]
Adapted from http://www.alternet.org

The interest in hemp has increased, mainly due to the new information about health effects of
phytocannabinoids. In addition, hemp seeds are rich in high nutritional value PUFA-rich oil
and proteins with good amino acid profile. Moreover, properly selected processes of multistep
hemp biorefining may provide several fractions, including antioxidant phytochemical and
dietary fibre rich products.

Keywords:
Hemp, phytocannabinoids, PUFA, Proteins, dietary fibers.

O. 8-2 Treating Sugar, Salt and Fat the Food Science Way

Dr. Pingfan Rao, is currently working as a Professor at Zhejiang Gongshang Pingfan Rao
University, China where he has been teaching for over six years. Dr. Pingfan Rao,
was graduated from Fuzhou University in 1982 and was admitted to study abroad Zhejiang Gongshang University, China
supported by the State Education Commission. In 1986, he received the Master’s
degree of food science from Hiroshima University and the doctor’s degree of Sharon Shoemaker, University of California, USA
biochemistry from Osaka Universityin 1989. He returned to Fuzhou University
in 1989 and worked in the Department of Light Industry (College of Biological ABSTRACT
Science and Engineering). He served as deputy director, director (dean) of the
department and the vice president of Fuzhou University. He took the lead in Sugar, salt and fat have had a long-lasting relationship with human beings. They
establishing and serving as director of the Institute of Biotechnology. His research have been passionately treasured and imaginatively used to create many fantastic
focuses primarily on identifying and characterizing bioactive proteins and foods. That has abruptly changed due to the alarming revelation of the association
expression and scale production of recombinant enzymes, protein derivatives as of excessive intake of sugar, salt and fat with deteriorating health and metabolic
the active ingredients of tradition Chinese medicine and food. In addition to his syndrome. Reduction of sugar, salt and fat is now the top public health priority in
work in China, he is also a Distinguished Visiting Professor of the University of many countries in the world. Is reduction or elimination the only way to deal with
Ulster, UK. He has published more than 100 scientific papers in various Peer- sugar, salt and fat? When sugar, salt and fat were food, none was ever used as a
reviewed Journals. He is a Fellow of the International Academy of Food Science pure compound. With clues of all the health benefits widely believed but not
and Technology and a President of International Union of Food Science & scientifically validated, exploring differences between unrefined sugar, salt and
Technology. fat and pure ones may provide a solution to the salt, sugar and fat challenge, and
push food science frontiers beyond basic chemical analysis. On the other hand,
technological development has made those precious food ingredients easily
available at low cost, resulting in increased use and cravings, and increased intake
which in turn resulted in reduced sensitivity, requiring much higher dosage to
achieve the same sensory pleasure. Facing this impossible challenge to reduce
sugar, salt and fat content in food for such a population with decreased sensitivity,
surprisingly, food science, and maybe only food science, can provide a simple
and brilliant solution. Raising sensory sensitivity is an established practice in
sensory science as a cornerstone of food science. Incorporating taste literacy
education into general education like music and physical training will empower
human beings with new capabilities. Facing health challenges, lets treat sugar, fat
and salt, the most important food ingredients, and ourselves the food science way,
which is gentler, rational, and hence more effective.

Keywords:

Sugar, salt, fat, craving, food science, education.

O. 8-3 The influence of daily diet on DNA methylation: Food as an
epigenetic factor
Dr. Jun Nishihira, is currently holding a position as Vice President and Director
of the Center of Health Information Science Center, Hokkaido Information Jun Nishihira*, Kouji Satoh, Hiroki Hattori, and Seiji Saito
University, Japan. He graduated from Hokkaido University School of Medicine
in 1979. In 2006, he took a post of professor of Hokkaido Information University, Department of Medial management and Informatics, Hokkaido Information University,
and currently vice president. He specializes in diabetes in clinical medicine, and Nishinopporo, Ebetsu City, Hokkaido 069-8585, Japan
most of the research time spent on the clinical trial for functional foods. He was
awarded as a recipient of the Innovation net award for the community health by ABSTRACT
the Ministry of Japan for Education, Culture, Sports, Science and Technology in
2016, and also honored of Hokkaido Science and Technology Award by Hokkaido DNA methylation at Cytosine (C) of the CpG sequence may be caused by various
municipal government for his science activities in food science in 2017. He has environmental factors. We investigated the change of degree of DNA methylation
received award like “The Innovation Net Awards” by the Ministry of Education, in association with the life-style, particularly diet and exercise. Subjects (5 males,
Culture, Sports, Science and Technology of Japanse Government and “The 6 females) participated in the 52-week study. The degree of methylation of C
Science and Technology Awards” of Hokkaido Municipal Government. He portion of the CpG sequence was examined. The degree of methylation of target
conducted several symposium or conferences including International Symposium regions was calculated by the equation; Demethylation degree = number of
of MIF, Sapporo in 2009, Food Summit 2011 in Ebetsu, Hokkaido, 2011 and demethylated CpG (T) /number of target CpG. We identified major changes of
International Forum on Food and Healthcare 2013 in Hokkaido, Sapporo in 2013 methylation in beta-3 adrenergic receptor (ADRb3) gene. In male, degree of
and International Forum on Health and Nutrition 2016 in Hokkaido, Ebetsu, 2016. methylation was changed in 8 weeks, which strongly correlated with increase of
He also published more than 250 SCI index research articles. blood pressure in male. We also found the increase in methylation as BMI and
visceral fat levels increased. The control of blood pressure is critical to keep us
healthy from arteriosclerosis leading to vascular injuries. The current result gives
us a hint to lower the risk of vascular injuries by monitoring the methylation of
ABRb3. Moreover, the methylation of ABRb3 would be used as the biomarker
to monitor visceral fat obesity. It remains to be investigated why and how the
methylation phenomenon takes place in male.

Keywords:
ABRb3, CpG, epigenetics, hypertension, methylation

O. 8-4 Possibilities of developing a functional sponge-fat cake with high
nutritional value and sensory quality
Dr. Katarzyna Świąder, is an Assistant Professor at the Faculty of Human
Nutrition and Consumer Science, Warsaw University of Life Sciences-SGGW Katarzyna Świąder1*, Anna Piotrowska1, Justyna Obiała
(WULS-SGGW), Warsaw, Poland. Dr. Katarzyna Świąder, received her
Doctorate from Warsaw University of Life Sciences-SGGW and worked as an 1 Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences –
Assistant at the same university and later become Assistant Professor. She is a SGGW, Nowoursynowska 159 C, 02-776 Warsaw, Poland
food expert, specialist in the field of functional food, sensory evaluation and new
product development. She is a certified dietician. The author or co-author of ABSTRACT
patents, several new food products implementations on the Polish and European
market, publications, elaborations and projects. International academic lecturer. The aim of the study was to evaluate the possibility of developing a functional
A member of the Polish Society of Nutritional Sciences and Polish Coeliac sponge-fat cake with high nutritional value and sensory quality by reducing the
Society. She was the Polish representative of the working group of the European sucrose content and increasing the protein content. The basic recipe of the cake
Commission, DG (SANCO) and CMDv in the European Medicines Agency. She was modified by gradually replacing sucrose with xylitol or isomaltulose at the
has been repeatedly awarded for her scientific and didactic achievements. Dr. following levels: 25%, 50%, 75%, 100% and by addition of protein preparations
Katarzyna Świąder, has participated in several international and national (whey protein concentrate, albumin, demineralized whey, whey or skim milk) at
conferences/symposiums and presented her work. She also has 2 patent food the assumed levels: 5%, 10%, 15%. The sensory evaluation was carried out with
products. She also published numerous research paper in various popular peer- expert method, the nutritional value of the new product was calculated, and the
reviewed Journals. possibility of using nutrition claims was defined. The optimal recipe was sponge-
fat cake with 100% sucrose replacement with xylitol and 15% addition of whey
protein concentrate. The cake was characterized by a relatively high sensory
quality and a high protein content, which allowed the use of the "protein source"
claim. Although the addition of albumin at the level of 15% (and 100% sucrose
replacement by xylitol) influenced the largest increase in protein content, its
sensory quality was significantly the lowest of the analyzed cakes. Functional
sponge-fat cakes can be intended for physically active people, elderly people,
diabetics and diet-conscious consumers.

Keywords:
Sugar reduction, protein, sponge cake, product development, functional cake.

O. 8-5 Nanodelivery system development for phyto compounds and its application
in brain disease treatments
Dr. Palanivel Ganesan, is an Assistant Professor at the Department of Integrated
Bioscience-Biomedical chemistry and Nanotechnology Research Center, Palanivel Ganesan
Konkuk University, Korea. He has received PhD 2010 in Food Science and
Technology (Food Biochemistry) at the Faculty of Agro-Industry, Prince of Department of Integrated Bioscience-Biomedical Science, Nanotechnology Research Center,
Songkla University, Thailand; and did his postdoctoral training at Sejong Konkuk University, Chungju 27478, Korea
University, South Korea and University Putra Malaysia, Malaysia, 2010-2015.
He is currently Assistant Professor at the Department of Integrated Bioscience- ABSTRACT
Biomedical chemistry, Konkuk University, Korea. His major areas of research
are on the delivery of plant-based bioactive compounds through micro or nano Nano delivery systems play a critical role in the enhanced delivery of wide range
delivery systems and to develop functional foods for various chronic disease of food grade bioactive compounds in various disease treatments including brain
treatments. He has more than 60 peer reviewed research articles including ACS diseases like Parkinson’s, Alzheimer’s and so on. The delivery of the food grade
Nano letters, Scientific Reports, International Journal of Nanomedicine, Drug bioactive compounds in crossing the blood brain barrier was enhanced through
Delivery, LWT-Food Science and Technology, Food Hydrocolloids, etc. He is our developed nanodelivery system like solid lipid nanoparticles (SLNs). In
also serving as an Editorial Board Member of scientific journals such as Frontiers compare to the other delivery systems, SLNs have excellent application in
in Nutrition, Frontiers in Chemistry, Beverages, Molecules etc. He has received treatment of neurodegenerative diseases. The multiple benefits of SLN delivery
several awards like National Research Fellowship Award, Grant Award for a includes improved stability, smaller particle size, leaching prevention, and
participation at a Meeting abroad, Thailand-Malaysia Grant Award, CFTRI enhanced lymphatic uptake of the bioactive compounds through oral delivery.
Invited Special Lecture Award. Also, presented his research data in several However, the burst release makes the SLN delivery systems inadequate for the
international conferences and symposiums. oral delivery of various phyto-bioactive compounds that can treat such chronic
diseases. Recently, the surface modification of solid-lipid nanoparticles (SMSLN)
was observed to overcome the above limitation for oral delivery of phyto-
bioactive compounds, and there is growing evidence that an enhanced uptake in
the brain of phytobioactive compounds delivered orally via SMSLN.

Keywords:

Solid-lipid nano particles, surface-modified solid-lipid nanoparticles, chronic
diseases, phyto-bioactive compounds

O. 9-1 Anti-allergy effect of nobiletin from citrus peel and
development of functional food
Dr. Takuya Sugahara, is currently a Professor at Faculty of Agriculture, Ehime
University as well as a Director of Food and Health Sciences Research Center, Takuya Sugahara1,*, Kosuke Nishi1, Momoko Ishida1
Ehime University, Japan. He received his PhD degree in Agriculture from
Graduate School of Agriculture, Kyushu University, Japan and joined as an 1 Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan;
Assistant Professor at Faculty of Agriculture, Ehime University and eventually
become Associate Professor and Full Professor. His research interest is evaluation ABSTRACT
of food functions (from Citrus fruits), and development of functional foods using
animal cell culture technique as well as pre-clinical (animal) and clinical trial Some food ingredients are expected to provide alternative and complementary
(human). His research team have evaluated health functions of many kinds of effects to prevent and alleviate the allergic symptoms to maintain and improve
foods, and published more than 140 papers. His research team recently developed the quality of life. We have screened an array of food extracts and ingredients for
the anti-allergy yogurt containing citrus peel in collaboration with food their ability to prevent degranulation using rat basophile leukemia RBL-2H3 cells
companies. He has joined several International and National conferences/ and found nobiletin, citrus peel flavonoids and β-lactoglobulin. RBL-2H3 cells
congress and presented his research data. He has guided several graduate students sensitized with IgE antibody were treated with nobiletin, and degranulation of the
during his 25 years of academic career. Dr. Takuya Sugahara, is in the Editorial cells was induced by challenging with the antigen. Enzyme released β-
Board member of several international Journals related to Food Science and hexosaminidases along with degranulation was determined as a degranulation
Nutrition. marker. The molecular mechanisms underlying their degranulation-inhibitory
activities were evaluated by immunoblot analysis. In vivo activities were
evaluated using mouse models of Japanese cedar pollinosis. As a result, nobiletin
suppressed degranulation of RBL-2H3 cells in a dose-dependent manner without
cytotoxicity. The synergistic effect of nobiletin and β-lactoglobulin on anti-
degranulation was evaluated, because we thought to develop functional yogurt
containing citrus peel. As a result, concurrent effect was clearly observed.
Immunoblot analysis showed that nobiletin and β-lactoglobulin interact with the
phosphoinositide 3-kinase and Syk pathways, respectively. Both concurrent
administration significantly suppressed pollinosis symptoms in vivo.

Keywords:
Nobiletin, β-lactoglobulin, pollinosis, degranulation, basophile leukemia RBL-
2H3 cells.

O. 9-2 Development of functional foods from edible seaweeds: moving forward
approval for functional health claim
Dr. You-Jin Jeon, is a Professor at the Faculty of Marine Biomedical Science at
Jeju National University in Korea, South Korea. He has received his B.S., M.S. You-Jin Jeon
and Ph.D. degrees from Pukyong National University, Busan, Korea and
conducted his postdoctoral research studies at Memorial University of Department of Marine Life Science, Jeju National University, South Korea
Newfoundland and University of Alberta in Canada. Dr. Jeon served as the
director of Marine and Environmental Research Institute in Jeju National ABSTRACT
University. He is working with marine biomass to develop functional food
materials, biomedical materials and marine cosmeceutical materials, and then he Recently consumers interested in the health enhancing role of specific foods with
has published around 300 international and 100 Korean domestic manuscripts, physiologically- active components, so-called functional foods, are increasingly seeking to
and about 35 patents regarding those research areas. He has been appointed to a control their own health and well-being. The Ministry of Food and Drug Safety in Korea has
member of the Korean Academy of Science and Technology. And he won many introduced "Health Functional Food Act", which is the policy to approve functional foods or
prizes from Jeju National University, including the best research, teaching and food ingredients with health claims based on sound scientific evidence with safety and human
project funding awards as well as Korean governments, the Ministry of Science intervention study being reliable, randomized and placebo-controlled. Geographically, Korea
and Technology and industry awads from Sajo Fisheries Company and Oddoogi has access to diverse marine bio-resources that need to be managed and utilized sustainably.
Food Company. The number of his lab members is currently 20 including five With a part of the project funded by the Ministry of Ocean and Fisheries in Korea, we are
post-doc, ten Ph.D. candidate students. During his academic career, he has guided working on marine algae and their components which are known to have different biological
many students/scholars. activities. In this research, the standardized marine derived resources with their specific
component are investigated for their effects on the molecular mechanisms responsible for their
biological activity in in-vitro and in-vivo studies, as well as their safety in these models. This
presentation would like to introduce the potential of marine resources as functional foods or
food ingredients as well as the blood glucose control effect of the extract from Ishige okaemura,
a brown edible seaweed to obtain health functional food claim from KFDA.

Keywords:
functional foods, marine algae, biological activity, glucose control, Ishige okaemura.

O. 9-3 Mushrooms as functional food and dietary supplement - type of extracts
and what is the right purification level
Dr. Miomir Niksic, is Professor of Food, Industrial Microbiology and Mushroom
Science, Chief of the Department of Food and Industrial Microbiology, Faculty Jovana Vunduk1, Dunja Miletić1, Danka Matijašević1, Anita Klaus1, Maja Kozarski1, Dragica
of Agriculture, University of Belgrade, Serbia. He received his masters and Jakovljević2, Željko Žižak3, Miomir Nikšić*1
Doctorate at the University of Belgrade. He has over 35 years of teaching
experience working with undergraduate, graduate and postgraduate students with 1Institute for Food Technology and Biochemistry, Faculty of Agriculture, University of
microbial control of food, mushrooms science and applied mycology and over 30 Belgrade, Nemanjina 6, Belgrade 11080, Serbia 2Institute of Chemistry, Technology and
years of professional experience in solving technological troubleshooting trace Metallurgy, University of Belgrade, Njegoseva 12, Belgrade 11001, Serbia 3Institute of
back problems in food and pharmaceutical companies. Dr. Miomir Niksic,
research group involved in developing a novel mushroom products Oncology and radiology of Serbia, Pasterova 14, Belgrade 11000, Serbia
(nutraceuticals) for food and pharmaceutical industry and also an active
consultant in cultivation of medicinal and industrial mushrooms. He is also doing ABSTRACT
training and evaluation of specialist in food microbiology, food hygiene, food
safety and hygienic engineering & design. He is senior international consultant More than 2000 mushrooms have antimicrobial, antioxidative, antitumor,
of UN World Bank and IFC in Food Safety, in policy development, in drafting immunomodulatory and other activities and fruiting body and mycelia are
legislation as well in supporting institutional reforms. He has published more than recognized as potential functional food and dietary supplement. The antibacterial
250 research articles in Peer-reviewed Journals. He is an active member of activity of methanol extract obtained from fruiting body of Coriolus versicolor
various society/Association including Microbiological Society of Serbia, The was examined. Mushroom expressed bactericidal activity against Gram (+) and
Serbian Association of Food Technologist. He is a General Secretary of Serbian Gram (-) bacteria and new approach in understand the mechanisms were involved.
Society for Nutrition and was a President of Serbian Mycological Society. Water polysaccharide extracts of two mushrooms, Auricularia auricula-judae
and Sparassis crispa, were subjected to the hydrolysis of α-D-glucoside bondages,
and dialyzed for further purification. The pure β-glucans were subjected to a
several in vitro biological activity tests, inhibition of angiotensin I-converting
enzyme (ACE), antimicrobial and cytotoxic activity. Susceptibility of 12
pathogenic bacteria was tested and appears to be significantly reduced or
completely lost when the purification of polysaccharides was performed. ACE
inhibition potential was reduced almost two times after α-glucans from the
extracts of both mushrooms were removed. All samples showed some different
activity toward selected tumor cell lines.

Keywords:
Mushrooms, Coriolus versicolor, Auricularia auricula-judae, Sparassis crispa,
glucans

O. 9-4 Bioactive components and health benefits of black garlic

Dr. Hao Jing, is currently working as a Professor at the College of Food Science Hao Jing
& Nutritional Engineering China Agricultural University (CAU) Beijing, P.R. College of Food Science and Nutritional Engineering, China Agriculture University,
China. Dr. Hao Jing, has graduated from Nanjing Medical University in 1982
with M.D. degree; graduated from the Academy of Military Medical Sciences Beijing 100083, P.R. China
with his master's degree in 1987; graduated from the Beijing Medical University
with Ph.D. degree in 1993; graduated from the University of British Columbia ABSTRACT
(UBC), Canada, with his second Ph.D. degree in 2003. Dr. Hao Jing then worked
as a senior research fellow of the Japan Society for the Promotion of Science Garlic (Allium sativum L.) has been used as food, spice, and traditional Chinese
(JSPS) in the Shimane University and the Shinshu University, in 2004 and 2005, medicine. Its characteristic pungent flavor limited its application in food product
respectively, in Japan. Dr. Jing has been a full professor China Agriculture development. Japanese scientist first, then followed by Korean scientist reported
University since 2006. His field of interest are find a novel functional food against the transformation production from garlic to black garlic under certain
various metabolic syndrome especially cancer. He also published numerous temperature and relative humidity. We have developed a new production
research papers in high impact journals. Dr. Hao Jing, has supervised many technology based on Maillard reaction, and shorted the processing time of black
students and act as reviewer for various international journals. garlic from 30d to 3d. Black garlic tastes slightly sweet without pungent flavor,
and has extended shelf-life. Black garlic has strong antioxidant activity with high
contents of phenolic-like and SOD-like substances. The organosulfur compounds
of alliin and allicin are very low, and S-allylcysteine is very high in black garlic.
Maillard reaction products have also been identified in black garlic. During the
thermal process, garlic fructan degraded into oligosaccharide, fructose and
glucose, which react with amino acids in garlic through Maillard reaction. The
intermediate products such as Amadori/Heyns products and late stage products
melanoidins were also identified. Bioactive properties of garlic include
antimicrobial, anti-cancer, anti-atherosclerotic, immune-modulatory activities.
Similar effects have also been reported for black garlic. More research needed to
identify the bioactive components of black garlic and associated health benefits.

Keywords:
Black garlic, Maillard reaction, bioactive, health.

O. 9-5 Chemical Composition and Biological Activity of
Processed Vietnamese Ginseng – A Potential Valuable Nutraceutical
Dr. Minh Duc Nguyen, is a Professor and Dean at Faculty of Pharmacy, Ton
Duc Thang University, Ho Chi Minh City, Vietnam. He received his Doctorate Thi Hong Van Le1, Gwang Jin Lee2, Sung Won Kwon2, Ngoc Khoi Nguyen1, Jeong Hill
from Hiroshima University, Japan in Pharmacognosy. Dr. Minh Duc Nguyen, Park2 , Minh Duc Nguyen1,3*
also worked as a Post-Doctoral Fellow at Hiroshima University, Japan. Then
moved back to University of Medicine and Pharmacy, Vietnam and Joined back 1 Faculty of Pharmacy, University of Medicine and Pharmacy Ho Chi Minh City, Vietnam
as lecturer and become Full Professor (1980-2015) and finally moved to Ton Duc 2 College of Pharmacy, Seoul National University, Korea
Thang University. His research interest covers Pharmacognosy & Natural
products like Ginseng & ginseng products as well as its application in 3 Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam
pharmaceutical industry for commercialization of these herbal products. Dr. Minh
Duc Nguyen, is in the Editorial Board of several renowned international Journals. ABSTRACT
He has been honored and awarded by many societies and associations in Vietnam.
He has guided several graduate students and published more than 160 articles in Vietnamese ginseng (VG), Panax vietnamensis Ha et Grushv., was discovered at Kontum
domestic and international journals. province in Middle Vietnam in 1973 as a new Panax species. The underground part of VG
contains a characteristic composition with more than 60 kinds of saponins. Besides
protopanaxadiol (PPD) and protopanaxatriol (PPT) ginsenosides as in other Panax spp., VG
possesses lots of ocotillol-type saponins, of which majonoside-R2 is the major one (yield over
5%). VG showed pharmacological activities similar with those of Panax ginseng such as
physical strengthening, anti-fatigue, CNS stimulating, anti-stress, immuno-stimulating, anti-
hyper glycemic, androgenic/estrogenic effects, etc. In recent studies, chemistry and biological
activities of heat-processed VG were studied. The result revealed steaming VG at 105 oC or
120 oC not only caused changes of its saponin composition but also its biological activities.
Besides the saponins found in VG, a series of newly formed less polar saponins were isolated
and identified including N-R2, 20 (S) & 20 (R) G-Rg3, 20 (S) & 20 (R) G-Rh1. G-Rh4, G-Rk1,
G-Rk3, and G-Rk5. Regarding biological activities, steaming process was proved to enhance
anti-proliferative activity on lung cancer cell A549 and antioxidant activity of VG. From
processed VG, panaxynol was isolated in high yield. The polyacetylene compound was shown
to inhibit Hsp90 function and viability of both non-CSC and CSC populations of NSCLC in
vitro and in vivo. Major ocotillol triterpenes isolated from VG including M-R2, P-RT4 and OCT
also showed inhibitory effects on inflammation in LPS-stimulated macrophages via the NF-κB
signaling pathway and significantly suppressed interleukin (IL)-6/tumor growth factor -
induced differentiation of splenic CD4+ T cells into Th17 cells and expression of IL-17 in vitro.

Keywords:

Vietnamese ginseng, saponins, ocotillol, lung cancer, inflammation.

O. 10-1 Effects of different proportions of dietary fat & carbohydrate on gut
microbiota and faecal metabolites and their relationship to cardiometabolic
Dr. Duo Li, currently working as a Chief-Professor at Institute of Nutrition &
Health, Qingdao University, 308 Ningxia Road, Qingdao 266071, China. Dr. Duo risk factors: a 6-month randomised controlled-feeding trial
Li, graduated (doctorate) from RMIT University, Australia. He also currently
working as an Emeritus Professor at Department of Food Science & Nutrition, Duo Li
Zhejiang University, China as well as Adjunct Professor at Nutrition and
Dietetics, Monash University, Australia. His major research field are related to Institute of Nutrition & Health, Qingdao University
vegetarianism, functional lipids and non-communicable diseases, nutrigenetics
and nutrigenomics. Dr. Duo Li have published 355 papers in peer reviewed ABSTRACT
journals with 13 book chapters and 24 inventive patents. Supervised as primary
supervisor 19 PhD and 22 MSc students. Received several awards globally To investigate whether diets differing in fat content (20%, 30% and 40% energy) alter the gut
includes Asia Pacific Clinical Nutrition Society Award, Contributing Award of microbiota, faecal metabolomic profiles, and plasma proinflammatory factors in healthy adults.
Student’s Nutrition and Health Task 2004, Turner A and Sinclair AJ. 1st Prize of In a 6-month randomised-controlled feeding trial, 307 healthy participants were assigned to
Natural Science Papers. Dr. Duo Li, is an Editor-in-Chief of Asia Pacific Journal one of the three isocaloric diets: a lower-fat diet (fat 20% energy, n=101), a moderate-fat diet
of Clinical Nutrition as well as Co-Editor of Asia Pacific Journal of Clinical (fat 30% energy, n=105), and a higher-fat diet (fat 40% energy, n=101) for 6 months. The
Nutrition, Statistics Editor of British Journal of Nutrition, Associate Editor of responses to the dietary interventions on the gut microbiota, faecal metabolomics, and plasma
Food & Function, Associate Editor of Journal of Nutrigenetics and inflammatory factors were investigated. Weight change at 6 months was −1.6 kg (95% CI −1.8
Nutrigenomics, Editor of Vegetarian Nutrition Journal, Editorial board of Annals to −1.4) in the lower fat, higher carbohydrate group; −1.1 kg (95% CI −1.3 to −0.9) in the
of Nutrition and Metabolism and so on. He also organised several conferences moderate fat, moderate carbohydrate group, and −0.9 kg (95% CI −1.1 to −0.6) in the higher
and Symposium. fat, lower carbohydrate group. Reduction in waist circumference, total cholesterol, low-density
lipoprotein cholesterol and non-high-density lipoprotein cholesterol on the lower fat, higher
carbohydrate group were greater than those observed on the other two diet groups. When
compared with the higher fat diet, the lower fat diet was associated with increased α-diversity
assessed by the Shannon index (p=0.03), increased abundance of Blautia (p=0.007) and
Faecalibacterium (p=0.04) at the genus level, whereas the higher-fat diet was associated with
increased Alistipes (p=0.04) and Bacteroides (p<0.001) and decreased Faecalibacterium
(p=0.004). Compared with lower-fat diet and moderate-fat diet, the concentration of total short
chain fatty acids was significantly decreased in the higher- fat diet group (p<0.001). The co-
metabolites p-cresol and indole, the precursor of p-cresol sulfate and indoxyl sulfate known to
be associated with host metabolic disorders, were decreased in the lower-fat diet group. In
addition, the higher-fat diet was associated with faecal enrichment in arachidonic and
lipopolysaccharide biosynthesis pathway as well as plasma proinflammatory factors after the
intervention. A lower fat, relatively higher carbohydrate diet, similar in macronutrient
composition to that traditionally eaten in China appears to be less likely to promote excessive
weight gain and be associated with a lower cardiometabolic risk profile than a diet more typical
of that eaten in Western countries in healthy nonobese Chinese. Higher fat consumption by
healthy young adults whose diet is in a state of nutrition transition appears to be associated
with changes in gut microbiota, faecal metabolomic profiles and plasma proinflammatory
factors with potential adverse consequences to long-term health outcomes.

Keywords:

gut microbiota, faecal metabolomic profiles, proinflammatory factors, p-cresol sulfate.

O. 10-2 Phytochemicals: their effects on diabetes, hyperuricemia and
skin photoaging
Dr. Kazumi Yagasaki, is a Professor (project) at Center for Bioscience Research
and Education, Utsunomiya University, Tochigi 321-8505, Japan and was a Kazumi Yagasaki1,3*, Shin-ichi Adachi1, Shinji Kondo1, Fumiaki Yoshizawa2,3
professor at Graduate School of Agricultural Science, Tokyo University of
Agriculture and Technology, Tokyo, Japan. Dr. Kazumi Yagasaki, studied 1Center for Bioscience Research and Education, Utsunomiya University, Tochigi, Japan 2 School
physiological chemistry and graduated from the University of Tokyo (Faculty of of Agriculture, Utsunomiya University, Tochigi 321-8505, Japan 3Graduate School of
Pharmaceutical Sciences), and completed his Ph.D. in nutritional chemistry at the
University of Tokyo (Graduate School of Agricultural Sciences). He studied as a Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan
visiting scientist at Karolinska Institutet, Stockholm. He was the Ex-president of
Japan Society of Nutrition and Food Science (JSNFS). He received JSNFS Award ABSTRACT
for Excellence in Research (2007), Japan Prize of Agricultural Science (2015),
JSNFS Award for Distinguished Service (2015) and others. Dr. Kazumi Yagasaki, The incidence of type 2 diabetes (T2D) is increasing worldwide. We have found
has guided several number of undergraduate, master and doctorate students. He that some phytochemicals (enterolactone derived from lignans, taxifolin) present
his field of interest are finding a novel nutraceuticals/ Functional food against in strawberry show antidiabetic actions in T2D model mice and increase glucose
various metabolic syndrome like anti-hyperglycation (Anti-diabetic), anti-cancer, uptake by cultured L6 myocytes through activation of AMPK and/or Akt, and
hypolipidemic activities. He has published more than 100 international research hence promote the translocation of glucose transporter 4 to plasma membrane.
papers in high index Journals. Hyperuricemia is well known as an important risk factor for gout. Recently, a
positive correlationship has been reported between the high blood uric acid (UA)
levels and insulin resistance (IR), this leading to aggravation of T2D or vice versa.
We have recently contrived assay systems for screening food and natural
substances that have antihyperuricemic activity: uric acid production in cultured
AML12 hepatocytes and purine bodies-induced hyperuricemic model mice.
Phytochemicals from strawberry and other resources have been found to suppress
UA production in the hepatocytes and hyperuricemia in the model mice by
inhibiting xanthine oxidase, a key enzyme in UA synthesis in the liver. We have
found that T2D model KK-Ay mice show hyperuricemia, and that taxifolin is
capable of suppressing this hyperuricemia as well as IR. Strawberry extract will
be addressed to alleviate skin photoaging induced by UVB irradiation in hairless
mice.

Keywords:

Diabetes, hyperuricemia, photoaging, myocyte, hepatocyte

O. 10-3 Chemopreventive effects of bioactive phytochemicals on chronic
inflammation-associated human diseases
Dr. Min-Hsiung Pan, is currently a Distinguished Professor and Graduate Chair
at Institute of Food Science and Technology, National Taiwan University, Taipei, Min-Hsiung Pan
Taiwan. He received his PhD at National Taiwan University (NTU), Taiwan and
worked as a Postdoctoral Associate in the same University. Later he moved to Institute of Food Science and Technology, National Taiwan University, Taiwan.
National Kaohsuing Marine University (NKMU) and become Assistant and
Associate Professor. In 2007, he has been promoted as Professor at NKMU and ABSTRACT
then finally move back to NTU in 2013 and become a Chair of Institute of Food
Science and Technology, NTU. His major field of interest includes cancer Over the past few decades, inflammation has been recognized as a major risk
biology (Novel chemotherapeutic agents especially against colon and breast factor for various human diseases. Acute inflammation is short-term, self-limiting
cancer), obesity, hyperlipidemia and diabetes mellitus. He received many awards and it's easy for host defenses to return the body to homeostasis. Chronic
such as Outstanding Research Award of National Kaohsiung Marine University, inflammatory responses are predisposed to a pathological progression of chronic
Outstanding Research Award of National Science Council, Outstanding Research illnesses characterized by infiltration of inflammatory cells, excessive production
and Technology Development Award of Health Food Society of Taiwan, of cytokines, dysregulation of cellular signaling and loss of barrier function.
Outstanding reviewer Award of Journal of Agriculture and Food Chemistry. He Targeting reduction of chronic inflammation is a beneficial strategy to combat
has many patents registered in different countries. He also published numerous several human diseases. Phytochemicals are widely present in the average diet in
research papers in National and International Journals. He also guided many such foods as fruits and vegetables, and have been demonstrated to exhibit a broad
graduates (Undergraduate and Master/Doctorate students) during his academic spectrum of biological activities for human health including an anti-inflammatory
tenure. property. Numerous studies have proposed that phytochemicals act through
variety mechanisms to prevent and attenuate inflammatory responses and serve
as possible cardioprotective, neuroprotective and chemopreventive agents. In this
review, we summarize current knowledge and underlying mechanisms on anti-
inflammatory activities of flavonoids and their implicated effects in the
development of various chronic inflammatory diseases.

Keywords:
Chemoprevention, phytochemicals, chronic inflammation