Honeywell Fluka™

NEW BRANDS! Discover the perfect formula Honeywell Research Chemicals แบรนดผูŒผลิตสารเคมีคุณภาพสูง (High-Quality Products) และรีเอเจŒนทสําหรับการ วิเคราะห (Analytical reagents) ซึ่งมีแหล‹งผลิตที่เมือง Seelze ประเทศเยอรมนี และดŒวย ประสบการณการผลิตและการควบคุมคุณภาพของสารเคมีมากว‹า 200 ป‚ ตั้งแต‹ ค.ศ.1814 โดยนักวิทยฯ ชาวเยอรมัน Johann Daniel Riedel ผูŒใชŒสามารถมั่นใจไดŒเลยว‹าเราสามารถ รักษามาตรฐานและคุณภาพของสินคŒาไดŒเปšนอย‹างดี ป˜จจุบันมีสินคŒาต‹างๆ ที่น‹าสนใจ เช‹น Hydranal, HPLC, LC-MS, GC-HS Solvents Advanced Manufacturing Capabilities สารเคมีความบริสุทธิ์สูง สําหรับใชŒงานเฉพาะทาง รวมถึงงานดŒานเทคนิคโครมาโตรกราฟ‚ เช‹น LC-MS, UHPLC, headspace และ GC เปšนตŒน สารเคมีอนินทรียและรีเอเจŒนทสําหรับใชŒงานดŒาน การวิเคราะหที่หลากหลาย เช‹น กรด, เบส, เกลือ, pH Buffersและสารมาตรฐาน รวมทั้ง Hydranol สําหรับใชŒวัดปริมาณนํ้า Overview Strength Capabilities and personnel - The site covers more than 485,000 m2 - Over 600 employees, with many in specialist R&D positions - Distillation units from lab scale (2 L) up to 6,000 L - Fully automatic filling line under laminar flow box to ensure quality - All high-purity grades filtered at the point of filling - High-quality packaging technology - Exceptional lot-to-lot consistency - High-purity hydrogen fluoride production - Organic and inorganic bulk production of fine chemicals for the pharmaceutical and agricultural industries - Wastewater treatment - A fully-equipped, in-house analytical department - Operates at the highest standards - TS 16949, ISO 9001, ISO 14001, OSHA 18001, and ISO 50001 accredited

NEW BRANDS! Code Name CAS No. Pack 33209-2.5L Acetic acid glacial 99.8% puriss. p.a. ACS Reag. ISO Reag.Ph.Eur (Glass bottle) (Fluka) 64-19-7 2.5 L 49199-50ML Acetic acid eluent additive for LC-MS (Fluka) 64-19-7 50 ml 32201-2.5L Acetone 99.5% puriss. p.a. ACS Reag.Ph.Eur (Glass bottle) (Riedel-de Haën™) 67-64-1 2.5 L 31146-1KG Boric Acid 99.8% puriss. p.a. ACS Reag. (Fluka) 10043-35-3 1 Kg 34741-1L Karl Fischer Hydranal™ Methanol Dry (Fluka) 67-56-1 1 L 31494-100G Silver sulfate, 99.5% puriss. p.a., ACS Reag. (Fluka) 10294-26-5 100 g 31434-1KG Sodium chloride 99.5% puriss. p.a., ACS Reag. (Fluka) 7647-14-5 1 Kg 30620-1KG Sodium hydroxide pellets 98-100.5% puriss. p.a., ACS Reag. (Fluka) 1310-73-2 1 Kg 239313-1KG Sodium sulfate anhydrous >99% Granular, ACS Reag. (Fluka) 7757-82-6 1 Kg 32249-2.5L Toluene 99.7% puriss. p.a. ACS Reag. Ph.Eur (Glass bottle) (Riedel-de Haën™) 108-88-3 2.5L 30743-2.5L Sulfuric acid 95-97% puriss. p.a. for determining Hg, ACS Reag. (Glass bottle) (Fluka) 7664-93-9 2.5 L 30702-2.5L Nitric acid 69-70% puriss. p.a.ACS Reag. ISO (Glass bottle) (Fluka) 7697-37-2 2.5 L 31404-500G Potassium permanganate 99% puriss. p.a., ACS, Reag.Ph.Eur (Fluka) 7722-64-7 500 g 32203-2.5L Diethyl ether 99.8% puriss. p.a.ACS Reag.Ph.Eur (Glass bottle) (Riedel-de Haën™) 60-64-1 2.5 L 30721-2.5L Hydrochloric acid 37% puriss. p.a.ACS Reag.ISO, Reag.Ph.Eur (Glass bottle) (Fluka) 7647-01-0 2.5 L 34805-1L Karl Fischer "Hydranal" Composite 5-Free from pyridine Single component (Fluka) 67-56-3 1 L 34807-500ML Karl Fischer Hydranal™ Coulomat A (Fluka) - 500 ml 34836-500ML Karl Fischer Hydranal™ Coulomat AG (Fluka) - 500 ml 34840-50ML Karl Fischer Hydranal™ Coulomat CG (Fluka) - 10x5 ml 34446-40ML Karl Fischer Hydranal™ Water Standard 0.1 mg/g (Fluka) - 10x4 ml 34843-500ML Karl Fischer Hydranal™ Coulomat AG-H (Fluka) - 500 ml Product List * มีสต็อคสินคŒาจําหน‹ายตั้งแต‹ เดือน ตุลาคม พ.ศ.2561 เปšนตŒนไป

Sample COA 1 CERTIFICATE OF ANALYSIS Wunstorferstrasse 40 Honeywell Specialty Chemicals Seelze GmbH Production date: 15.Mar.2018 Best before: 26.Aug.2021 Page: Product: 33209 Seelze, 30926 Lot No: I0740 Telefon: +49 5137 999-0 Brand: Honeywell Fluka™ www.lab-honeywell.com Parameter Specification Result Units Acetic acid min. 99,8 %, puriss. p.a., Reag. ACS, Reag. ISO, Reag. Ph. Eur. Units assay 99.8-100.5 % 100.0 % assay (GC) min. 99.8 % 99.8 % density (D 20/20) 1.052-1.053 1.052 - 1.053 boiling range 117 - 119 °C 117 - 119 °C congealing - freezing point min. 16.3 °C >16.3 °C non-volatile matter max. 0.0005 % <0.0005 % water (Karl Fischer) max. 0.4 % <0.01 % silver (Ag) max. 0.000001 % <0.000001 % aluminium (Al) max. 0.000005 % <0.000005 % arsenic (As) max. 0.000001 % <0.000001 % barium (Ba) max. 0.000001 % <0.000001 % beryllium (Be) max. 0.000001 % <0.000001 % bismuth (Bi) max. 0.00001 % <0.00001 % calcium (Ca) max. 0.00002 % <0.00002 % cadmium (Cd) max. 0.000002 % <0.000002 % cobalt (Co) max. 0.000001 % <0.000001 % chromium (Cr) max. 0.000005 % <0.000005 % copper (Cu) max. 0.000001 % <0.000001 % iron (Fe) max. 0.00002 % <0.00002 % germanium (Ge) max. 0.000005 % <0.000005 % potassium (K) max. 0.00001 % <0.00001 % lithium (Li) max. 0.000001 % <0.000001 % magnesium (Mg) max. 0.00001 % <0.00001 % manganese (Mn) max. 0.000001 % <0.000001 % molybdenum (Mo) max. 0.000002 % <0.000002 % sodium (Na) max. 0.00005 % <0.00005 % Page : 1

Sample COA Page: 2 Parameter Specification Result Units Acetic acid min. 99,8 %, puriss. p.a., Reag. ACS, Reag. ISO, Reag. Ph. Eur. Units nickel (Ni) max. 0.000005 % <0.000005 % lead (Pb) max. 0.000002 % <0.000002 % strontium (Sr) max. 0.000001 % <0.000001 % titanium (Ti) max. 0.000001 % <0.000001 % thallium (Tl) max. 0.000005 % <0.000005 % vanadium (V) max. 0.000001 % <0.000001 % zinc (Zn) max. 0.000005 % <0.000005 % zirconium (Zr) max. 0.00001 % <0.00001 % heavy metals (as Pb) max. 0.00005 % <0.00005 % chloride (Cl) max. 0.00005 % <0.00005 % phosphate (PO4) max. 0.00005 % <0.00005 % sulfate (SO4) max. 0.00005 % <0.00005 % acetaldehyde max. 0.0002 % <0.0002 % acetic anhydride (GC) max. 0.01 % <0.01 % mixable with H2O complying complying titrable base max. 0.0004 meq/g <0.0004 meq/g APHA max. 10 <10 appearance of the substance complying complying K2Cr2O7 red. matter complying complying KMNO4 red. substances complying complying Tel. 02-138-3720-3, 061-823-3761-3, 083-959-9593 Fax. 02-138-3724-5 E-mail : [email protected] Website : www.apexchemicals.co.th APEX CHEMICALS CO., LTD. บร�ษัท เอเพกซ เคมิเคิล จำกัด Page : 2 CERTIFICATE OF ANALYSIS

Research Chemicals HYDRANAL™ CRM Water Standards for Karl Fischer Titration Honeywell Fluka™ is pleased to present a new generation of Hydranal Certified Reference Material (CRM) Water Standards. These CRMs are produced and certified under ISO Guide 34 in combination with ISO/IEC 17025, using volumetric or coulometric Karl Fischer Titration. The products are supplied with a printed certificate containing a certified value, comprehensive documentation, proper uncertainty calculation, lot-specific values, expiration dates, and storage information. Karl Fischer Titration is the most widely used method for measuring water content in products and substances. Today, highly accurate measurements are a requirement for many end users. This change, which is driven by regulatory authorities, is forcing many laboratories to acquire ISO/ IEC 17025 accreditation. To help laboratories meet this requirement and obtain highly-accurate measurements and traceability in their results, lab managers can now turn to Honeywell’s new product line of Hydranal CRMs. Hydranal CRMs serve as a reference point. They are traceable to high-purity materials of well-established purity and/or SI units. Additionally they are verified against internationally accepted standards from national metrological institutes such as NIST (National Institute of Standards & Technology, USA) or NMIJ (National Metrology Institute of Japan). In addition to traceability to accepted references, Hydranal CRMs have a well-defined mass fraction and highly accurate measurement of uncertainty which can be found in the Certificate of Analysis (CoA). Hydranal CRMs are tested under ISO/IEC 17025 and comply with ISO Guide 34 requirements. Bulk Production Filling in Final Containers Accelerated Stability Test (AST) Minimum Sample Amount Long-term Stability Test (LTS) Sampling Content Determination Verification Against External Reference Materials e.g. NIST SRM 2890, NMIJ 4222-a Traceability either directly to SI unitor to high purity materials Homogeneity Testing Certified Value & Uncertainty Review and Certification Certified Reference Material according to ISO/IEC 17025 and ISO Guide 34 General flowchart for the certification process of Hydranal™ CRM

PRODUCT NUMBER PRODUCT NAME DESCRIPTION PACKAGING 34424 Solid CRM standard, water content ~ 15.66% 10 g 34425 Liquid CRM standard, water content 10.0 mg/g = 1.0%10 x 8 mL 34426 Liquid CRM standard, water content 1.0 mg/g = 0.1%10 x 4 mL © 2017 Honeywell International Inc. All rights reserved. To learn more about Hydranal, visit hydranal-honeywell.com AMERICAS Honeywell Corporate Headquarters 115 Tabor Road Morris Plains, NJ 07950 Manufacturing Facility 1953 South Harvey Street Muskegon, Ml 49442 EUROPE Honeywell Specialty Chemicals Seelze GmbH Manufacturing Facility Wunstorferstrasse 40 30926 Seelze, Germany ASIA/PACIFIC Asia Pacific Headquarters Honeywell (China) Co. Ltd. 430 Li Bing Road Pudong New Area Shanghai 201203 Honeywell Specialty Chemicals New Pier Takeshiba, South Tower Building Minato-ku, Tokyo, Japan 1050022 Fluka and Hydranal are trademarks of Honeywell Specialty Chemicals Seelze GmbH. All statements and information provided herein are believed to be accurate and reliable, but are presented without guarantee, warranty or responsibility of any kind, express or implied. Statements or suggestions concerning possible use of our products are made without representation or warranty that any such use is free of patent infringement, and are not recommendations to infringe any patent. The user should not assume that all safety measures are indicated herein, or that other measures may not be required. User assumes all liability for use of the information and results obtained. 02-138-3720-3,061-8233761-3 หรอื 083-959-9593 @ [email protected] www.apexchemicals.co.th APEX CHEMICALS CO., LTD. (Head Office) 2034/87, Italthai Tower 19th Floor, New Petchburi Road, Bangkapi, Huaykwang, Bangkok 10310, Thailand Tel. 02-038-9999 Fax. 02-038-0099 @ [email protected] f ApexChemicals Line @ApexChemicals www.apexchemicals.co.th

HydranalTM Karl Fischer titration Original Accuracy Hy = KFt Ori xAcy Discover the perfect formula HYDRANAL™ Product Overview Guide Reagents for water determination by Karl Fischer titration Research Chemicals

HYDRANAL Product Line at a Glance HYDRANAL™ Reagents from Honeywell Research Chemicals Product Line Product Description HYDRANAL-Composite The most flexible and commonly used reagents for one-component volumetric titration HYDRANAL Special Media Special reagents like Methanol Rapid, E-Types and K-Types HYDRANAL-Titrant/Solvent Reagents for two-component volumetric titration HYDRANAL-Coulomat Reagents for coulometric titration for samples with low water content HYDRANAL-Water Standards Standards with verified water content for titer determination, monitoring precision, accuracy, validation and inspection of Karl Fischer titrators HYDRANAL-CRM Water Standards Certified Reference Materials for titer determination, monitoring precision, accuracy, validation and inspection of Karl Fischer titrators Honeywell is your partner for reliable and easy-to-use pyridine-free Karl Fischer reagents Advantages of HYDRANAL Reagents: • High titration speed • Stable end points • Accurate results • Long shelf life • Wide applicability • World leading technical support 1 Water content can affect product quality, texture, shelf life, chemical stability and reactivity. Karl Fischer titration is a universally accepted method for measuring water content in all types of substances, including chemicals, oils, pharmaceuticals and food. In 1979, researcher Dr. Eugen Scholz improved Karl Fischer titration by replacing noxious pyridine with imidazole. This innovation became the foundation of Hydranal™, the world’s leading pyridine-free reagents for Karl Fischer titration. From Dr. E. Scholz’s pioneering research to the ongoing product improvements of today, Honeywell offers a wide range of Karl Fischer reagents for both volumetric and coulometric titrations for nearly all types of samples, completed by a broad range of standards. With the inclusion of Fluka™ into Honeywell Research Chemicals, Hydranal became an important part of the overall product portfolio. Hydranal reagents and water standards have always been developed and produced in our plant in Seelze, Germany, meaning you can be sure to enjoy the same composition, quality, service and technical support you always have.

The Chemistry of Karl Fischer Titration The Karl Fischer technique for water determination, invented in 1935 by Karl Fischer, is a titration based on the Bunsen reaction. In 1979 it was postulated by Dr. E. Scholz as a two-step equation: The oxidation of alkylsulfite to alkylsulfate in reaction (2) consumes water, which ideally comes only from the sample. Since water and iodine are consumed in a 1:1 stoichiometric ratio, the amount of water in the original sample is calculated by the amount of iodine required to complete the reaction. The iodine is measured either volumetrically or coulometrically. How the Base Affects Reaction Kinetics The type of base (R’N) and its concentration affect the overall reaction rate. Traditionally, pyridine was used as the base. However, because of its weak basicity, pyridine cannot completely neutralize the alkyl-sulfurous acid intermediate. As a result, reaction (1) is slow, does not go to completion and the end point is not stable. Because of this lack of stability, the repeatability of the results is often very poor. In addition, pyridine has a noxious odor. Imidazole and 2-Methylimidazole as Alternatives to Pyridine Dr. E. Scholz and his research team sought to replace the pyridine with a stronger base with a higher affinity for the alkylsulfite. Imidazole was found to have even more benefits than pyridine besides not having the noxious odor. Imidazole allows reaction (1) to go to completion rapidly and provides a stable end point. Later on researchers found that adding a second base, 2-methylimidazole, to the imidazole, enhances stability and reduces the appearance of undesired crystallization. 2 (2) [R’NH]SO3 R + H2 O + I 2 + 2R’N→2[R’NH]I + [R’NH]SO4 R (1) ROH + SO2 + R’N ↔ [R’NH]SO3 R ROH = alcohol, typically methanol R’N = base

Volumetric One-Component Titration - Titrating Agents HYDRANAL-Composite Hydranal-Composite is the world‘s most frequently used pyridine-free Karl Fischer reagent. This one-component reagent has been proving its capabilities in volumetric titration for more than thirty five years in a large range of applications in the most diverse fields of research and industry. Ongoing development work has achieved significant improvements to this reagent. Improved Composition Hydranal-Composite contains all the reactants including iodine, sulfur dioxide, and the bases imidazole and 2-methylimidazole, dissolved in diethylene glycol monoethyl ether (DEGEE). Adding 2-methylimidazole in addition to imidazole improves the stability and eliminates the formation of crystals which can interfere with the titrator’s performance. The crystallization of the reagent was occasionally observed under the influence of airborne moisture and also after prolonged residence of the reagent in the Karl Fischer titrator‘s tube system. This effect is prevented by a new and improved formulation. Enhancement of Titer Stability When comparing the old and new formulation it becomes obvious that the new formulation is significantly more stable with a loss of concentration less than 5% per year vs. approx. 10% for the old formulation. Hydranal-Composite is additionally stabilized with DEGEE as a solvent. The results of the tests into titer decline are shown in Figure 1. Advantages of HYDRANAL One-Component Reagents: • Unlimited water capacity • Convenient and easy to use • The greatest flexibility in working media selection • Suitable for methanol-reacting compounds, e.g. ketones and aldehydes • Long shelf life (three years) Product Number Product Name Description Packaging 34827 HYDRANAL-Composite 1 One-component reagent, titer ~1 mg/mL 500 mL; 1 L 34806 HYDRANAL-Composite 2 One-component reagent, titer ~2 mg/mL 500 mL; 1 L; 2.5 L 34805 HYDRANAL-Composite 5 One-component reagent, titer ~5 mg/mL 500 mL; 1 L; 2.5 L 34816 HYDRANAL-Composite 5 K One-component reagent for titration of ketones and aldehydes, titer ~5 mg/mL 500 mL; 1 L; 2.5 L 5.6 5.5 5.4 5.3 5.2 0 30 60 90 120 150 180 Time [days] New Formulation Old Formulation Titer [mg/mL] Figure 1. Results of the titer stability tests HYDRANALComposite Media 3

Volumetric One-Component Titration - Media With one-component reagents the medium (i.e. the solvent required) is chosen according to the dissolution properties of the sample substance being analyzed. The most commonly used medium is dry methanol. Product Number Product Name Description Packaging 37817 HYDRANAL-Methanol Rapid Medium containing accelerators 1 L; 2.5 L 34741 HYDRANAL-Methanol Dry Medium for general use 1 L; 2.5 L 34734 HYDRANAL-CompoSolver E Ethanol-based medium containing accelerators 1 L; 2.5 L 34697 HYDRANAL-Solver (Crude) Oil Working medium containing methanol, xylene and chloroform for titration in oils 1 L; 2.5 L 37855 HYDRANAL-LipoSolver CM Working medium containing methanol and chloroform for titration in non-polar samples 1 L 37856 HYDRANAL-LipoSolver MH Working medium containing methanol and 1-hexanol for titration in non-polar samples 1 L 34698 HYDRANAL-Medium K Less toxic working medium containing chloroform for ketones and aldehydes 1 L 34738 HYDRANAL-KetoSolver Working medium free of halogenated solvents for ketones and aldehydes 500 mL; 1 L 34817 HYDRANAL-Working Medium K Working medium containing chloroform and 2-chloroethanol for ketones and aldehydes 1 L HYDRANAL-Methanol Rapid The speed, time taken and accuracy of the Karl Fischer reaction is influenced by the medium used in the titration vessel. The HydranalComposite, one-component reagents, are already buffered to an optimum of pH by using imidazoles. Thus the performance of the titrating agent is optimized to ensure a rapid Karl Fischer titration, however, there is still room for improvement on the use of the solvent. Methanol is the most commonly used medium in the titration vessel, however it is an unbuffered solvent. When using Hydranal-Methanol Rapid, you will see a clear improvement in speed and accuracy of the titration. This is due to the accelerators in the medium, which are unique to HydranalMethanol Rapid and enable an optimal Karl Fischer titration (see Figure 2). HYDRANAL-CompoSolver E In case a less toxic solvent is preferred, Hydranal-CompoSolver E, an ethanol based medium, has a similar performance to Hydranal-Methanol Rapid. HYDRANAL-Solver (premixed) Many non-polar samples (e.g. oils, fats, organic components) appear with a poor solubility in methanol and require the addition of a solubilizer. To overcome these challenges, a series of specially designed media has been developed based on the most suitable solvent mix. HYDRANAL-K Media For compounds reacting with methanol, like ketones and aldehydes, three different media have been developed. Comparing the three media based on their toxicity and capacity to suppress side effects, we recommend the use of Hydranal-Medium K as the first choice. Figure 2. Titration of 20 mg water 4.0 3.0 2.0 1.0 0.0 0 100 200 300 Time [s] Volume [mL] Methanol Rapid Methanol Dry ▼ ▼ 4 Advantages of HYDRANALMethanol Rapid: • Much shorter titration time • Rapid end point • High accuracy of the analysis

Volumetric Two-Component Titration HYDRANAL-Titrant / Solvent Composition In two-component reagents the Karl Fisher reactants are separated into two solutions: the titrant and the solvent. Hydranal-Titrant contains iodine dissolved in an alcohol with a precisely defined concentration. Hydranal-Solvent is an alcoholic solution of sulfur dioxide and imidazole. The alcohol is either methanol for standard reagents or ethanol for E-type reagents. Titrant Solvent Product Number Product Name Description Packaging Methanol Based 34811 HYDRANAL-Titrant 2 Two-component reagent, titer ~2 mg/mL 500 mL; 1 L; 2.5 L 34801 HYDRANAL-Titrant 5 Two-component reagent, titer ~5 mg/mL 500 mL; 1 L; 2.5 L 34800 HYDRANAL-Solvent Working medium for two-component titration 1 L; 2.5 L Ethanol Based 34723 HYDRANAL-Titrant 2 E Two-component reagent, titer ~2 mg/mL 1 L 34732 HYDRANAL-Titrant 5 E Two-component reagent, titer ~5 mg/mL 500 mL; 1 L; 2.5 L 34730 HYDRANAL-Solvent E Working medium for two-component titration 500 mL; 1 L; 2.5 L Special Media 34812 HYDRANAL-Solvent CM Working medium for two-component titration, containing methanol and chloroform for titration in non-polar samples 1 L; 2.5 L 34749 HYDRANAL-Solvent Oil Working medium for two-component titration, containing methanol and 1-hexanol for titration in non-polar samples 1 L 34697 HYDRANAL-Solver (Crude) Oil Working medium containing methanol, xylene and chloroform for titration in oils 1 L; 2.5 L 5 Advantages of HYDRANAL Two-Component Reagents: • High titration speed • Ideal accuracy for small amounts of water • High buffer capacity • Exact and stable titer • E-type reagents: reduced toxicity compared to methanol • Long shelf life (three years for titrants, five years for solvents)

Coulometric Titration HYDRANAL-Coulomat Coulometric Karl Fischer titrations normally require two reagent solutions: an anolyte (the solution in the anodic compartment) and a catholyte (the solution in the cathodic compartment). Hydranal-Coulomat A-type or E-type reagents are used as anolytes. The anolytes contain iodide and a sulfur dioxide/imidazole buffer in suitable solvents. Hydranal-Coulomat CG reagents are used as catholytes. Coulometric reagents based on different solvent compositions serve to support the broad variety of samples analyzed, i.e. Hydranal-Coulomat Oil contains methanol, xylene and chloroform for titration in oils or methanol-free HydranalCoulomat AK for titration of ketones. Furthermore, some working techniques are supported by special reagents i.e. the use of Karl Fischer oven by HydranalCoulomat AG-Oven or the use of a cell without diaphragm by HydranalCoulomat AD. Coulometric Cells There are two different types of coulometric cells: those with and those without a diaphragm. The diaphragm separates the anode chamber from the cathode chamber. Oxidation of I⁻ to I² occurs at the anode, whereas the reduction of protons to H² occurs at the cathode. For cells without a diaphragm the anodic and cathodic compartments are not separated and only one reagent, the anolyte, is needed. Though the latter coulometric cell may seem more convenient to use, the cell with diaphragm achieves the highest accuracy (down to a trace range of water). * In theory all Hydranal-Coulomat anolytes may be used with either type of generator electrode: with or without a diaphragm. However, the anolytes which contain a co-solvent in addition to methanol show increased recoveries when used with a diaphragmless generator electrode. Therefore we recommend using a diaphragm generator electrode when working with a co-solvent containing anolyte. This will require the use of the appropriate catholyte. Advantages of HYDRANAL Coulometric Reagents: • Easy to use • High accuracy for trace amounts of water • Stable conditions of the titration vessel • Broad product range • Long shelf life (up to five years) A C HYDRANALCoulomat (Catholyte) HYDRANALCoulomat (Anolyte) Product Number Product Name Description Packaging 34807 HYDRANAL-Coulomat A Anolyte preferred for cells with diaphragm* 500 mL 34836 HYDRANAL-Coulomat AG Anolyte suitable for cells with and without diaphragm 500 mL; 1 L 34843 HYDRANAL-Coulomat AG-H Anolyte for titration of long-chained hydrocarbons, preferred for cells with diaphragm* 500 mL 34739 HYDRANAL-Coulomat AG-Oven Anolyte for determination with Karl Fischer oven, suitable for cells with and without diaphragm 500 mL 34820 HYDRANAL-Coulomat AK Anolyte for titration of ketones, preferred for cells with diaphragm* 500 mL 34868 HYDRANAL-Coulomat Oil Anolyte for titration of oils, preferred for cells with diaphragm* 100 mL; 500 mL 34726 HYDRANAL-Coulomat E Anolyte based on ethanol, suitable for cells with and without diaphragm 500 mL 34810 HYDRANAL-Coulomat AD Anolyte preferred for cells without diaphragm 500 mL 34840 HYDRANAL-Coulomat CG Catholyte 10 x 5 mL 34821 HYDRANAL-Coulomat CG-K Catholyte for titration of ketones 10 x 5 mL 6

Titer Standardization and Instrument Inspection HYDRANAL-Water Standards Quality management plays an important role in Karl Fischer titration. Calibration, validation and inspection of analytical instruments and reagents is performed with a specific amount of water, either pure water or water standards. The challenge with pure water is the low amount required (10-50 mg for volumetry, and 0.1-1 mg for coulometry), which is difficult to handle and weigh. We therefore recommend Hydranal-Water Standards with an exactly confirmed water content for: • Titer determination • Monitoring precision and accuracy • Validation and inspection of Karl Fischer titrators according to ISO, GMP, GLP and FDA guidelines Traceability to a national standard or to a SI unit is often required in these guidelines. All Hydranal-Water Standards are tested against the NIST (National Institute of Standards and Technology, USA) standard reference material SRM 2890, Water Saturated Octanol. Liquid standards consist of a solvent mixture with specific composition and precisely determined water content. They are packaged in glass ampoules under argon. Each box contains ten single-use ampoules which are easy to open (pre-notched). Solid standards contain defined amounts of chemically bound water suitable for both general use as well as for the Karl Fischer oven. These standards are packed in amber glass bottles. HYDRANAL-CRM Water Standards In 2014, Hydranal Technical Service in Seelze completed its combined accreditation according to ISO/IEC 17025 and ISO Guide 34, the so-called “Gold Standard Accreditation”, which is the highest achievable quality level for producers of Certified Reference Materials (CRMs). With the double accreditation, Hydranal introduced the very first commercially available CRM Water Standards for Karl Fischer titration. Advantages of HYDRANAL-Water Standards: • Broad product range for volumetric and coulometric Karl Fischer applications • Manufactured according to current ISO requirements • Tested against NIST SRM 2890 • Long shelf life (up to five years) • Convenient packaging • Supplied with detailed instruction for use • Report of Analysis showing the exact water content is included 7

Product Number Product Name Description Packaging 34425 HYDRANAL-CRM Water Standard 10.0 Liquid CRM standard, water content 10.0 mg/g = 1.0% 10 x 8 mL 34426 HYDRANAL-CRM Water Standard 1.0 Liquid CRM standard, water content 1.0 mg/g = 0.1% 10 x 4 mL 34424 HYDRANAL-CRM Sodium Tartrate Dihydrate Solid CRM standard, water content ~15.66% 10 g 34849 HYDRANAL-Water Standard 10.0 Liquid standard, water content 10.0 mg/g = 1.0% 10 x 8 mL 34828 HYDRANAL-Water Standard 1.0 Liquid standard, water content 1.0 mg/g = 0.1% 10 x 4 mL 34847 HYDRANAL-Water Standard 0.1 Liquid standard, water content 0.1 mg/g = 0.01% (shelf life 2 years, to be stored at 2-8°C) 10 x 4 mL 34446 HYDRANAL-Water Standard 0.1 PC Liquid standard water content 0.1 mg/g = 0.01% (improved stability compared to 34847: shelf life 3 years, to be stored at room temp.) 10 x 4 mL 34694 HYDRANAL-Water Standard Oil Liquid standard based on mineral oil, water content <50 ppm (0.005%) 10 x 8 mL 34696 HYDRANAL-Standard Sodium Tartrate Dihydrate Solid standard, water content ~15.66% 25 g 34693 HYDRANAL-Water Standard KF Oven 140-160°C Solid standard for control of Karl Fischer ovens, water content ~5%, based on lactose 10 g 34748 HYDRANAL-Water Standard KF Oven 220-230°C Solid standard for control of Karl Fischer ovens, water content ~5.55%, based on potassium citrate 10 g 8

Solubilizers Karl Fischer titration is applied to multifarious substances. The nuances in sample properties influence the Karl Fischer titration differently. There are a number of ways to adjust the working conditions in order to enable a direct titration of the sample and avoid complicated and error-prone pre-dissolution and pre-extraction steps. In some cases the addition of solubilizers is recommended. Buffers The Karl Fischer reaction is pH dependant, with pH 5-7.5 being the ideal range. Strongly acidic samples slow the reaction and must be neutralized without inducing an alkaline reaction of the working medium prior to starting the titration. Strong bases can increase the pH of the working solution if the basicity exceeds the buffering capacity of the reagent. A titration end point will not be reached. Strong bases also must be neutralized prior to starting the titration. HYDRANAL-Moisture Test Kit For rough measurements without a titrator, special test kits for visual water determination according to Karl Fischer can be used. The set contains syringes, titration vessel and reagents: 2 x 500 mL Hydranal-Solvent E (34730), 100 mL Hydranal-Titrant 5 E (34732) and 100 mL Hydranal-Standard 5.0 (34813). Refills can be ordered separately. Product Number Product Name Description Packaging 34724 HYDRANAL-Formamide Dry Solubilizer, max. 0.02% water 1 L 37863 HYDRANAL-Chloroform Solubilizer, max. 0.01% water 1 L 37866 HYDRANAL-Xylene Solubilizer, max. 0.01% water 1 L 34804 HYDRANAL-Buffer Acid Liquid buffer medium, based on imidazole 500 mL 37859 HYDRANAL-Buffer Base Liquid buffer medium, based on salicylic acid 1 L 32035 HYDRANAL-Benzoic Acid Buffer substance 500 g 37865 HYDRANAL-Salicylic Acid Buffer substance 500 g 37864 HYDRANAL-Imidazole Buffer substance 500 g 34813 HYDRANAL-Standard 5.0 Test solution for volumetric titration, water content 5.00 mg/mL 100 mL; 500 mL 34803 HYDRANAL-Sodium Tartrate Dihydrate Test substance for volumetric titration, water content ~15.66% 100 g 34802 HYDRANAL-Water-in-Methanol 5.0 Reagent for volumetric back titration, water content 5.00 mg/mL 500 mL; 1 L 34788 HYDRANAL-Humidity Absorber Drying agent for air and gases with indicator 500 g; 1 kg 34241 HYDRANAL-Molecular Sieve 0.3 nm Drying agent for air and gases 250 g 37858 HYDRANAL-Moisture Test Kit Test kit for the visual water determination according to Karl Fischer without titrator 1 kit 9 Auxiliaries for Karl Fischer Titration

Technical Support For more than 35 years, the Hydranal Technical Service Team has been gathering extensive and unmet experience and insights into Karl Fischer titration and its related challenges. If you are looking to improve your Karl Fischer titration performance, the team of Hydranal experts can support you with: • Selecting the most suitable Karl Fischer reagents for your samples • Recommending application methods • Troubleshooting technical problems (solubility, side reactions, etc.) • Technical Karl Fischer seminars and trainings • Comprehensive literature To learn more about Hydranal reagents, visit hydranal-honeywell.com Please, do not hesitate to contact us at [email protected] or contact our Hydranal specialists directly: Europe and Global Market Thomas Wendt HYDRANAL Center of Excellence Seelze, Germany Tel. +49 (0) 5137 999-353 E-Mail: [email protected] Europe and Global Market Agnieszka Kossakowska HYDRANAL Technical Specialist Warsaw, Poland Mobile: +48 512 355 628 E-Mail: [email protected] USA and Canada Doug Clark HYDRANAL Technical Center St. Louis, MO Toll free +1 800 493-7262 E-Mail: [email protected]

AMERICAS Honeywell Corporate Headquarters 115 Tabor Road Morris Plains, NJ 07950 Manufacturing Facility 1953 South Harvey Street Muskegon, Ml 49442 EUROPE Honeywell Specialty Chemicals Seelze GmbH Manufacturing Facility Wunstorferstrasse 40 30926 Seelze, Germany ASIA/PACIFIC Asia Pacific Headquarters Honeywell (China) Co. Ltd. 430 Li Bing Road Zhang Jiang Hi-Tech Park Pudong New Area Shanghai 201203 Honeywell Specialty Chemicals New Pier Takeshiba, South Tower Building 20th Floor, 1-16-1 Kaigan Minato-ku, Tokyo, Japan 1050022 Fluka and Hydranal are trademarks of Honeywell Specialty Chemicals Seelze GmbH. HYD-001-0004-ENG | 02/17v_14 © 2017 Honeywell International Inc. All rights reserved. All statements and information provided herein are believed to be accurate and reliable, but are presented without guarantee, warranty or responsibility of any kind, express or implied. Statements or suggestions concerning possible use of our products are made without representation or warranty that any such use is free of patent infringement, and are not recommendations to infringe any patent. The user should not assume that all safety measures are indicated herein, or that other measures may not be required. User assumes all liability for use of the information and results obtained. To learn more about Honeywell’s Research Chemicals Portfolio, visit lab-honeywell.com or email us at [email protected]

Advance your analysis Honeywell Fluka™ analytical standards offers a growing range of high quality inorganic and organic reference materials produced according to ISO 9001, and suitable for a wide range of industries and applications. Every vial comes complete with a printed Certificate of Analysis (CoA) and is eligible for free expert technical support - ensuring you have the information you need to quickly start your analysis, and pass relevant audits. Each CoA includes: 1. Contact and technical support details 2 . Expiry date 3. Product number, name and batch 4. General product information 5. Lot specific analytical results 6. Purity test by HPLC, GC, LC-MS, GC-MS and Titration 7. Identity test by NMR, FT-IR or Mass Spectrometry Research Chemicals Fluka™ Analytical Standards Advance your analysis with expert technical support 7 1. General Information Molar mass: 617.73 Recomm. storage temp.: roomtemp. Usage : CAS-No.: 2. Batch Analysis [84759-11-5] Reference Material (RM) Formula: C33H31D9N2O9 CERTIFICATE OF ANALYSIS Honeywell Specialty Chemicals Seelze GmbH D-30918 Seelze phone: +49 5137 999-150 Seelze, 27.Apr.2018 27.Apr.2021 05.06.2018 Production Date: Expiry Date: Reserpine-(3,4,5-trimethoxy-d9), reference material Article/Product: 0001R Batch: X711I / COAH_00377196/ GE61/18/09694 Date of Analysis: 27.Apr.2018 g/Mole CERTIFICATE OF ANALYS )RMN( ytitnedi gniylpmoc )CLPH( yassa 7.99 % aera ytniatrecnu tnemerusaem 5.0 ± % D yassa 99> % egnar gnitlem 572 - 372 C° Honeywell • The expiry date is based on the current knowledge and holds only for proper storage conditions in the originally closed flasks/ packages. • Whenever the container is opened for removal of aliqout portions of the substance, the person handling the substance must assure, that the integrity of the substance is maintained and proper records of all its handlings are kept. Special care has to be taken to avoid any contamination or adulteration of the substance. • We herewith confirm that the delivery is effected according to the technical delivery conditions agreed. • Particular properties of the products or the suitability for a particular area of application are not assured. • We guarantee a proper quality within our General Conditions of Sales. Quality Management This document was produced electronically and is valid without a signature 3. Advice and Remarks 6 1 2 3 4 5 APEX CHEMICALS CO., LTD. (Head Office) 2034/87, Italthai Tower 19th Floor, New Petchburi Road, Bangkapi, Huaykwang, Bangkok 10310, Thailand Tel. 02-038-9999 Fax. 02-038-0099 @ [email protected] f ApexChemicals Line @ApexChemicals www.apexchemicals.co.th

All Pharmaceutical & Veterinary Compounds ACE inhibitors Anesthetics Anthelmintics, Flukicides Antibacterial agents Antibiotics Anticoccidial Antiemetics Antiprotozoal agents Benzimidazoles beta-Agonist, Antagonists Coccidiostat Hydantoins Imidazoles Neuroleptics NSAIDs Polypeptides Retinoides Steroids, Hormones Sulfonamides Tranquilizers Pesticides & Metabolites Pharmaceutical & Veterinary Compounds All Pesticides & Metabolites Acaricides, Insecticides Fungicides, Bactericides Herbicides Molluscicides Plant growth regulators Rodenticides APEX CHEMICALS CO., LTD. (Head Office) 2034/87, Italthai Tower 19th Floor, New Petchburi Road, Bangkapi, Huaykwang, Bangkok 10310, Thailand Tel. 02-038-9999 Fax. 02-038-0099 @ [email protected] f ApexChemicals Line @ApexChemicals www.apexchemicals.co.th

Limit expensive delays with free expert technical support If you have a critical question that is not addressed by our documentation, Honeywell Fluka provide fast technical support from experts who have been developing, producing and testing standards for decades, ensuring you get timely, effective help with even the trickiest application questions – potentially saving you hundreds of Euros, and hours of lab-work*. • We s olve the majority of customer product enquiries after one email or phone call. • Mo re complex application troubleshooting is normally resolved within 5 working days. Save valuable lab-time using Honeywell custom standards Producing a standard in-house can be a technically challenging, time consuming and expensive process, requiring you to source materials, purify, and validate your standard. Our extensive experience enables us to efficiently produce and test custom standards to your requirements for less than it would cost to produce in-house**. • With all our facilities on one site, our expert team can develop, test and manufacture a simple custom standard within a week, and a more complex custom standard within two. • Be cause our custom standards are guaranteed to be the correct specification and come with full documentation, you can be sure your analysis is reliable and accurate. To browse our complete reference material range, or to request a quote for a custom standard visit lab-honeywell.com/standards. APEX CHEMICALS CO., LTD. (Head Office) 2034/87, Italthai Tower 19th Floor, New Petchburi Road, Bangkapi, Huaykwang, Bangkok 10310, Thailand Tel. 02-038-9999 Fax. 02-038-0099 @ [email protected] f ApexChemicals Line @ApexChemicals www.apexchemicals.co.th

1. General Information Molar mass: 304.30 Recomm. storage temp.: 2-8 °C Usage : Herbicide CAS-No.: 2. Batch Analysis [36001-88-4] Reference Material (RM) Formula: C11H17N2O4PS CERTIFICATE OF ANALYSIS Honeywell Specialty Chemicals Seelze GmbH D-30918 Seelze phone: +49 5137 999-150 email: [email protected] Seelze, 11.Jun.2018 11.Jun.2021 20.07.2018 Production Date: Expiry Date: Amiprofos methyl, reference material Article/Product: R1311 Batch: I162X / COAH_00394557/ GE61/18/15118 Date of Analysis: 19.Jul.2018 g/Mole identity (NMR) complying assay (GC) 99.9 area % measurement uncertainty ± 0.5 % melting range 66.2 - 67 °C water (Karl Fischer) <0.2 % Honeywell • The expiry date is based on the current knowledge and holds only for proper storage conditions in the originally closed flasks/ packages. • Whenever the container is opened for removal of aliqout portions of the substance, the person handling the substance must assure, that the integrity of the substance is maintained and proper records of all its handlings are kept. Special care has to be taken to avoid any contamination or adulteration of the substance. • We herewith confirm that the delivery is effected according to the technical delivery conditions agreed. • Particular properties of the products or the suitability for a particular area of application are not assured. • We guarantee a proper quality within our General Conditions of Sales. Quality Management This document was produced electronically and is valid without a signature 3. Advice and Remarks Uncertainty of Measurement มีการรายงานค‹าความไม‹แน‹นอนในการวัด เปšนสารมาตรฐานที่เปšน Reference Materials

ppm 0 2 4 6 8 10 12 14 16 Amiprofos methyl reference material #R1311 Lot: I162X Sample Name: 18_15118 Data Collected on: Seelze-NMR-vnmrs400 Archive directory: /home/vnmr1/vnmrsys/data Sample directory: 18_15118_20180719 FidFile: 18_15118_PROTON_02 Pulse Sequence: PROTON (s2pul) Solvent: cdcl3 Data collected on: Jul 19 2018 Temp. 26.0 C / 299.1 K Sample #3, Operator: vnmr1 Relax. delay 5.000 sec Pulse 45.0 degrees Acq. time 2.281 sec Width 7183.9 Hz 64 repetitions OBSERVE H1, 399.8812274 MHz DATA PROCESSING FT size 32768 Total time 7 min 46 sec

C H R O M A T O G R A M M 5 10 15 20 25 30 min pA 0 20 40 60 80 12.220 12.517 20.676 Area Percent Report # Meas. R Height Area Area % 1 12.220 841.638 5728.92 99.90 2 12.517 1.582 2.63 0.05 3 20.676 0.985 2.95 0.05 ------------------------------------------------------ Honeywell Specialty Chemicals Seelze GmbH Analytical Department Article : Amiprofos methyl Article-No : R1311 Lot : I162X Column : Rtx-5, 30m, 0,25mm i.D., 0,25µm Film Inj.-Temp. : 300°C Det.-Temp. : 320°C - FID Oven-Temp. : 100°C to 300°C (10°C/min) hold 15min Split : 1:100 Flow : 1ml He/min Inj.v. : 1µl solution (~5% in Dichloromethane) Evaluation : Normalisation (uncorrected) Operator : Florie

Uncertainty of Measurement มีการรายงานค‹าความไม‹แน‹นอนในการวัด เปšนสารมาตรฐานที่เปšน 1. General Information Reference Materials Molar mass: 532.46 Recomm. storage temp.: roomtemp. Usage : Antifungal CAS-No.: 2. Batch Analysis [67915-31-5] Reference Material (RM) Formula: C26H31Cl2N5O3 CERTIFICATE OF ANALYSIS Honeywell Specialty Chemicals Seelze GmbH D-30918 Seelze phone: +49 5137 999-150 email: [email protected] Seelze, 11.Jun.2018 11.Jun.2021 10.07.2018 Production Date: Expiry Date: Terconazole, reference material Article/Product: R1332 Batch: I162X / COAH_00390342/ GE61/18/14259 Date of Analysis: 10.Jul.2018 g/Mole identity (NMR) complying assay (HPLC) 99.6 area % measurement uncertainty ± 0.5 % melting range 128.1 - 128.4 °C water (Karl Fischer) 0.1 % Honeywell • The expiry date is based on the current knowledge and holds only for proper storage conditions in the originally closed flasks/ packages. • Whenever the container is opened for removal of aliqout portions of the substance, the person handling the substance must assure, that the integrity of the substance is maintained and proper records of all its handlings are kept. Special care has to be taken to avoid any contamination or adulteration of the substance. • We herewith confirm that the delivery is effected according to the technical delivery conditions agreed. • Particular properties of the products or the suitability for a particular area of application are not assured. • We guarantee a proper quality within our General Conditions of Sales. Quality Management This document was produced electronically and is valid without a signature 3. Advice and Remarks

ppm 0 2 4 6 8 10 12 14 16 Terconazole reference material #R1332 Lot: I162X Sample Name: 18_14259 Data Collected on: Seelze-NMR-vnmrs400 Archive directory: /home/vnmr1/vnmrsys/data Sample directory: 18_14259_20180706 FidFile: 18_14259_PROTON_01 Pulse Sequence: PROTON (s2pul) Solvent: cdcl3 Data collected on: Jul 6 2018 Temp. 26.0 C / 299.1 K Sample #7, Operator: vnmr1 Relax. delay 5.000 sec Pulse 45.0 degrees Acq. time 2.281 sec Width 7183.9 Hz 64 repetitions OBSERVE H1, 399.8812283 MHz DATA PROCESSING FT size 32768 Total time 7 min 46 sec

Article-No: Article: Batch: Column: Eluent A: Eluent B: Gradient: Flow: Detection: Injection-Vol: Sample-Preparation: Linearity: Evaluation: Operator: min mAU 0 1 2 3 4 5 6 0 25 50 75 100 PDA Multi 1 220nm,4nm 0,630 1,951 2,210 3,566 4,647 B.Conc <Peak Table> PDA Ch1 220nm Peak# 1 2 3 4 5 Total Ret. Time 0,630 1,951 2,210 3,566 4,647 Area 1372 574 1365552 1809 1327 1370633 Height 315 168 295499 737 217 296936 Area% 0,100 0,042 99,629 0,132 0,097 100,000 <Sample Information> R1332 Terconazole, reference material I162X Poroshell 120 EC C18 2,7μm 3,0*50mm Acetonitrile Water + 0,1% Phosphoric acid time %A %B 1 min 30 70 4 min 70 30 6 min Stop 0,8 mL/min UV - 220nm 10 μL 10 mg / 100mL ACN-H2O 30-70 checked Normalisation (uncorrected) Schowe Honeywell Specialty Chemicals -----Analytical Department-----

Research Chemicals Inorganic Trace Analysis TraceSELECT™ High Purity Reagents for Sample Preparation and Analysis Discover the perfect formula TraceSELECTTM Honeywell Highest purity Reliability Ts + Hw = Py2 + Ry

Sample Preparation for Trace Analysis: TraceSELECT™ Ultra and TraceSELECT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Trace Analysis: High Purity Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Solvents for Trace Metal Speciation Analysis: TraceSELECT solvents for LC-ICP-MS applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Definition of Speciation Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Ultra Pure Reagents for Voltammetry: TraceSELECT reagents for ultra-trace analysis and metal speciation . . . . . . . . . . . . . 10 Contents

1. Feng, X.; Wu, S.; Wharmby, A.; Wittmeier, A. Microwave digestion of plant and grain standard reference materials in nitric and hydrofluoric acids for multi-elemental determination by inductively coupled plasma mass spectrometry. J. Anal. At. Spectrom. 1999, 14, 939–946. 2. Golimowski J.; Golimowska, K. UV photooxidation as pretreatment step in inorganic analysis of environmental samples. Anal. Chim. Acta. 1996, 325, 111–133. 3. Vandecasteele, C.; Block, C. B. Modern Methods for Trace Element Determination; Wiley: Chichester, UK, 1997; pp 1–8. 4. Sampling and Sample Preparation; Stoeppler, M., Ed; Springer: Berlin, 1997. 5. Sah, R. N.; Miller, R. O. Spontaneous reaction for acid dissolution of biological tissues in closed vessels. Anal. Chem. 1992, 64, 230. 6. Inductively coupled plasmas in analytical atomic spectrometry; Montaser, A.; Golightly, D. W., Eds.; VCH: New York, 1992; pp 473–516. 7. Tracy, M. L.; Moeller, G. Continuous flow vapor generation for inductively coupled argon plasma spectrometric analysis; Part 1: Selenium. J. Assoc. Off. Anal. Chem. 1990, 73, 404–410. 8. Pacquette, H. L.; Elwood, S. A.; Ezer, M.; Simeonsson, J. B. A comparison of continuous flow hydride generation laser-induced fluorescence and laser enhanced ionization spectrometry approaches for parts per trillion level measurements of arsenic, selenium and antimony. J. Anal. At. Spectrom. 2001, 16, 152–158. 9. Sun, Y. C.; et al. Combining electrothermal vaporization inductively coupled plasma mass spectrometry with in situ TMAH thermochemolysis for the direct determination of trace impurities in a polymer-based photoresist. J. Anal. At. Spectrom. 2006, 21, 311–316. 10. Jeitziner, M. High-purity TMAH for alkaline digestion of biological samples. Analytix 2008, 1, 17. 11. Jeitziner, M. High-Purity Reagents for AAS. Analytix 2011, 1, 14. Reagents for Inorganic Trace Analysis Analysis you can trust 2 Sensitive trace analysis applications require extremely pure sample preparation reagents. These digestive reagents cannot contain metal ions or other impurities. In addition, complete decomposition of the sample is required to achieve reproducible and accurate elemental results by instrumental analytical methods. Sample wet digestion/dissolution is a method that breaks down the components of a matrix into simple chemical forms. This digestion can occur in three ways: w With the introduction of energy such as heat w By using a chemical reagent such as an acid w By a combination of these two methods Most analytical measurements using highly sensitive methods (AAS, ICP-AES, stripping voltammetry, ion chromatography, etc.) are performed on samples in solution. One of the most effective and economical sample preparation methods is microwave digestion. In most cases, achieving homogeneity and mineralization of the sample is sufficient. UV photolysis using hydrogen peroxide and either potassium persulfate or nitric acid is very often the method of choice for the decomposition of organic impurities in aqueous solutions. The most commonly used reagents for wet decomposition are mineral and oxidizing acids. Wet decomposition has the advantage of being effective on both inorganic and organic materials. It often destroys or removes the sample matrix, thus helping to reduce or eliminate some types of interference. Table 1 provides an annotated overview of the acids and bases used for wet digestion. With Honeywell’s analytical reagents, superior quality and safety standards are a prime focus. We use a comprehensive Quality Assurance System in line with EN 29001 (ISO 9001) and each of our products is manufactured to clear, guaranteed specifications. We employ a large variety of modern analytical methods, such as inductively coupled plasma atomic emission spectroscopy (ICP-OES), inductively coupled plasma mass spectrometry (ICP-MS) , flame atomic absorption spectroscopy (AAS) and graphite furnace AAS. This allows for specifically tailored control and analysis procedures for each product. Sources Atomic Emission Spectroscopy Analysis

3 HNO3 Nitric acid is the most frequently utilized sample dissolution medium. It oxidizes metals not dissolved by HCl and other non-oxidizing acids. Au, Pt metals (except Pd), Nb, Ta, and Zr are not dissolved. Al and Cr are passivated. Sn, Sb, and W give insoluble hydrous oxides. Dissolves most sulfides (except HgS). Unfortunately, the carbon contained in organic materials is only partly converted to CO2 by HNO3 at temperatures up to 200 °C. Nitric acid should never be used for the digestion of highly aromatic compounds because of the potential for the formation of highly explosive compounds. In the case of alcohols, the samples should be pretreated with sulfuric acid. HCl Hydrochloric acid is used for many salts of weak acids, e.g., carbonates, phosphates, some oxides, and some sulfides. H2SO4 Sulfuric acid is used when its high boiling point (300 °C) is an advantage, as in expelling a volatile product or increasing the reaction rate. It provides dehydrating and oxidizing properties at high temperatures. HClO4 Perchloric acid is a very powerful oxidizing agent at fuming temperatures (boiling point 203 °C). It is usually mixed with HNO3 to oxidize easily attacked organic matter that might otherwise react violently with HClO4. H2SO4 (dehydrating agent) increases oxidizing power. Good solvent for stainless steel and for sulfides. HF Hydrofluoric acid is used for digestion of silacaceous samples and as an auxiliary reagent to HNO3 or HClO4 to eliminate fluoride. With HNO3, HF dissolves Ti, W, Nb, and Zr (and their carbides, nitrides, and borides) as a result of formation of complex fluorides. Certain refractory silicates and other minerals are not decomposed; these must be dissolved by fusion. H2O2 Hydrogen peroxide is a very popular oxidizing reagent as it is converted to water and oxygen during the oxidation of biological material. Additional advantages are that there is no acid corrosion of the digestion vessel PTFE walls, no formation of insoluble salts with an acid anion, and no change of the sample matrix by an acid. Because of its strong oxidation power, only small amounts of H2O2 need be used so concentrated sample solutions can be obtained. AcOH Acetic acid is most often used for the extraction of metallic impurities together with sodium acetate. HBr Hydrobromic acid is a non-oxidizing acid. It is used in some special applications because it yields better reductive selectivity in the determination of Se(VI) by Hydride Generation-Laser Induced Fluorescence (HG-LIF) than HCl. H3PO4 Phosphoric acid is commonly used in the semiconductor industry to both clean and etch metal surfaces. The concentration of the phosphoric acid is critical to optimizing these processes. H2O Water ensures the highest accuracy in trace analysis of ppt range by minimizing blank values. TMAH Tetramethylammonium hydroxide [(CH3)4NOH] is an efficient strong alkali used for solubilizing soft tissues and food stuffs. Compared to conventional alkaline sample digestion procedures using NaOH or KOH, using TMAH enables more accurate analysis due to its lower matrix effect. A diluted 0.3 M TMAH solution is also used during the lithographic process in the semiconductor industry. NaOH Sodium hydroxide is frequently used for melting digestions at 500 °C. It attacks platinum and porcelain. Residue is dissolved with 12 M hydrochloric acid. KOH Potassium hydroxide is frequently used for melting digestions at 500 °C. It attacks platinum and porcelain. Residue is dissolved with 12 M hydrochloric acid. K2CO3 Potassium carbonate is the classic reagent for melting digestions at 800 °C. Residue is dissolved with hydrochloric acid. Digestion/Dissolution Acids and Bases Table 1

4 Sample Preparation for Trace Analysis TraceSELECT™ Ultra and TraceSELECT Sample preparation for trace analysis requires reagents of the highest purity. Our TraceSELECT Ultra products for ultra-trace analysis at ppb and even ppt levels are produced by sub-boiling distillation. Sub-boiling is recognized as the best way to obtain high purity acids with the lowest blank values for ultra-trace analysis. The technique is based on the evaporation of liquid by infrared heating at the surface. It avoids violent boiling and the formation of liquid aerosols that can be transported with the distillate. To maintain their high purity, TraceSELECT Ultra products are supplied in PTFE PFA (fluoropolymer) bottles. Water and ortho-phosphoric acid are supplied in especially pre-leached HDPE bottles. Recent process improvements have allowed us to reduce our impurity specifications to guarantee the lowest levels of trace impurities in our TraceSELECT Ultra products. The acids, bases, and salts in the TraceSELECT series have been developed for sample preparation and analysis in the ppb (μg/kg) trace range. Purity and composition are guaranteed with our careful preparation, testing, and verification of the final product for metal content and ionic trace impurities using ICP-OES, ICP-MS and ion chromatography. To further guarantee purity and stability, TraceSELECT products are packaged in high-quality containers appropriate for each product. The Honeywell Quality Management System guarantees consistent quality and safety for all TraceSELECT Ultra and TraceSELECT products. The reagents are produced and bottled under clean-room conditions. For more information, visit: lab-honeywell.com/traceselect Ultra-pure acids, bases, and salts for smelting and wet digestion in environmental, water, and food analysis. Honeywell Fluka - Ammonium Acetate TraceSELECT 73432-100G

5 Product Number Brand Product Name Specification Packaging 45727 Fluka Acetic acid ≥ 99.0% (T) 100 mL, 500 mL ,1 L, 2.5 L 09857 Fluka Ammonium hydroxide solution ≥ 25% in water (T) 100 mL, 500 mL 06454 Fluka Formic acid ≥ 88.0% (T) 250 mL 08256 Fluka Hydrochloric acid ≥ 30% (T) 100 mL, 500 mL , 1 L, 2.5L 84415 Fluka Hydrochloric acid ≥ 37% fuming (T) 100 mL, 500 mL 47559 Fluka Hydrofluoric acid 47-51% (AT) 100 mL, 500 mL 84385 Fluka Nitric acid > 69.0% (T) 250 mL, 500 mL, 1 L, 2.5L 77227 Fluka Perchloric acid 67-72% (T) 100 mL, 500 mL, 1 L 79614 Fluka Phosphoric acid ~ 85% (T) 100 mL, 500 mL 30955 Fluka Potassium hydroxide solution ≥ 30% in water (T) 250 mL 13171 Fluka Sodium hydroxide solution ≥ 30% in water (T) 250 mL 84716 Fluka Sulfuric acid ≥ 95% (T) 500 mL, 1 L, 2.5 L 68556 Fluka Tetramethylammonium hydroxide solution 25% in water 250 mL 95305 Fluka Water 250 mL, 500 mL, 1 L, 2.5 L, 10 L TraceSELECT Reagents Product Number Brand Product Name Specification Packaging 07692 Fluka Acetic acid >99.0% (T) 250 mL, 500 mL, 1 L 96208 Fluka Hydrochloric acid >30-35% 250 mL, 500 mL, 1 L 02650 Fluka Nitric acid >65-71% 250 mL, 500 mL, 1 L, 2 L 12415 Fluka Perchloric acid 67-72% (T) 250 mL, 500 mL, 1 L 64957 Fluka Phosphoric acid >85% (T) 250 mL, 1 L 77239 Fluka Sulfuric acid >95% (T) 250 mL, 500 mL, 1 L 14213 Fluka Tetramethylammonium hydroxide solution 25% in water 250 mL TraceSELECT Ultra Reagents Product Number Brand Product Name Specification Packaging 73432 Fluka Ammonium acetate ≥ 99.9995% 100 g 09725 Fluka Ammonium chloride ≥ 99.9995% 25 g, 100 g 09726 Fluka Ammonium phosphate monobasic ≥ 99.9999% 25 g, 100 g 09979 Fluka Ammonium sulfate ≥ 99.9999% 100 g 90033 Fluka Cesium chloride ≥ 99.9995% 25 g, 100 g 16722 Fluka Cesium iodide ≥ 99.9995% 25 g 62462 Fluka Lithium carbonate ≥ 99.998% 25 g, 100 g 60348 Fluka Potassium bisulfate ≥ 99.995% 25 g 60111 Fluka Potassium carbonate sesquihydrate ≥ 99.995% 50g TraceSELECT Salts

Matrix Modifiers In graphite furnace AAS, element determinations are increasingly being carried out with matrix modifiers. Chemical modification should be considered if an analyte is highly volatile, or if the analyte and matrix volatilize at similar temperatures. Such modification would allow ashing at higher (or atomization at lower) furnace temperatures, resulting in elimination of the matrix with no loss of the analyte (or atomization of the analyte, but not the matrix). Depending on the element to be determined, various substances are used. However, palladium nitrate has become one of the most popular. A primary criterion for such substances is the absence of the element to be analyzed. For this reason, the following recommended reagents have been specially tested for their suitability as matrix modifiers. Trace Analysis: High Purity Reagents Product Number Brand Product Name Specification Packaging 95164 Fluka Cesium chloride/Lanthanum chloride matrix modifier solution CsCl and LaCl3 250 mL, 500 mL, 1 L 428868 Fluka Calcium matrix modifier solution 2% Ca²+ in 1% HNO3 100 mL 428884 Fluka Lanthanum matrix modifier solution 5% La³+ in 1% HCl 100 mL 428892 Fluka Magnesium matrix modifier solution 2% Mg²+ in <5% HNO3 100 mL Matrix Modifiers 6 Melting digestion is used for solid samples like ores, rock, metals, alloys, ceramic, and cement, in order to obtain a homogenous residue, which can be dissolved in diluted TraceSELECT acids. Our TraceSELECT salts obtain a very high purity, with metal traces typically below 10 μg/kg (10 ppb). TraceSELECT Salts (continued) Product Number Brand Product Name Specification Packaging 05257 Fluka Potassium chloride ≥ 99.9995% 25 g, 100 g 60216 Fluka Potassium phosphate monobasic ≥ 99.995% 25 g, 100 g 60371 Fluka Potassium hydroxide hydrate ≥ 99.995% 25 g 30533 Fluka Potassium iodide ≥ 99.999% 100 g 60347 Fluka Potassium phosphate dibasic ≥ 99.999% 100 g 60429 Fluka Potassium nitrate ≥ 99.995% 25 g, 100 g 59929 Fluka Sodium acetate ≥ 99.999% 25 g, 100 g 71347 Fluka Sodium carbonate ≥ 99.9999% 25 g, 100 g 38979 Fluka Sodium chloride ≥ 99.999% 25 g, 100 g 01968 Fluka Sodium hydroxide monohydrate ≥ 99.9995% 25 g, 100 g 71752 Fluka Sodium nitrate ≥ 99.999% 25 g, 100 g 71629 Fluka Sodium phosphate dibasic ≥ 99.999% 100 g 71492 Fluka Sodium phosphate monobasic ≥ 99.999% 25 g, 100 g

7 Spectroscopic Buffers for Flame AAS In flame AAS, spectroscopic buffers are often used to suppress physical, ionization, and chemical interferences. The Schuhknecht and Schinkel buffer solution, as used in the determination of alkali elements, has become particularly important. A multi-element standard for lithium, sodium, and potassium is also available for this method. The Schinkel buffer solution enables the method to be expanded to include up to 14 mono- and divalent elements, with simple calibration being all that is required for the analysis. Reducing Agents for Hydride AAS Hydride AAS is used for the analysis (especially traces) of arsenic, antimony, tin, selenium, bismuth, and mercury. It is used to separate and preconcentrate analytes from sample matrices by a reaction that turns them into their hydride vapors. Sodium borohydride is the common reagent of choice for the reduction. Fluka reagents are specifically analyzed to ensure the absence of hydride generating metals. Product Number Brand Product Name Specification Packaging 20980 Fluka Aluminum nitrate-Cesium chloride buffer Buffer solution according to Schuhknecht and Schinkel 500 mL 20982 Fluka Cesium chloride-Lanthanum chloride buffer Buffer solution according to Schinkel 500 mL 95164 Fluka Cesium chloride-Lanthanum chloride buffer CsCl 100 g/L, LaCl3 100 g/L (in water) 250 mL Spectroscopic Buffers for Flame AAS 1. Schuhknecht, W.; Schinkel, H. Fresenius J. Anal. Chem. 1963, 161, 194. 2. Schinkel, H. Fresenius J. Anal. Chem. 1984, 10, 317. Product Number Brand Product Name Specification Packaging 71321 Fluka Sodium borohydride ≥ 99% 25 g, 100 g 30533 Fluka Potassium iodide ≥ 99.999% 100 g Reducing Agents for Hydride AAS

Min 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 8 Honeywell has developed high purity TraceSELECT solvents for speciation analysis by LC-ICP-MS. TraceSELECT solvents undergo rigorous purification procedures. This is followed by UV spectroscopy, IC, and ICP-MS testing to assure high chemical purity and high UV transmittance. The blank values for metal traces in these solvents are in the ppb range or lower. The toxicity of trace metals in food, clinical chemistry, biology, and environmental sciences is an area of increasing interest. In the environment, many diverse species of an element can be present. In addition, different species of the same element can possess very different degrees of toxicity. Monitoring elemental species requires an analytical method that is sensitive and specific enough to resolve and quantify the individual species at ultratrace levels. An individual toxic species may constitute only a fraction of an element’s total concentration in a sample. The coupling of the two well established analytical techniques, HPLC and inductively coupled plasmamass spectrometry (ICP-MS), is straightforward. This is primarily because the flow rates commonly used with LC are compatible to conventional liquid sample introduction systems, such as those based on pneumatic nebulization. Thus, the outlet of the LC column is directly connected to the ICP-MS nebulizer. This technique is especially useful in carrying out automated highthroughput speciation analysis. International trends show speciation of metals as an area of very high research interest. In the future, speciation of biological samples will undoubtedly involve determinations of which organic molecules attract which metals in specific sample types. ICP-MS is a superior detection technique for trace elements in general, but especially for elements of interest, such as arsenic, selenium, cadmium, iodine, and others in chromatographic eluents. TraceSELECT™ solvents for LC-ICP-MS applications Solvents for Trace Metal Speciation Analysis The chromatogram of three mercury species Hg2+, MeHg+ , EtHg+ obtained by HPLC-ICP-MS

9 TraceSELECT Solvents Ideal for Speciation Analysis Product Number Brand Product Name Specification Packaging 01324 Riedel-de Haën Acetonitrile ≥99.9% 1 L 04516 Riedel-de Häen 2- Propanol ≥99.9% 1 L 42105 Riedel-de Haën Methanol ≥99.9% 1 L 92328 Riedel-de Haën Ethylene glycol butyl ether ≥ 99.5% 1 L 69508 Riedel-de Haën Acetone ≥ 99.9% 1 L 72781 Riedel-de Haën N,N-Dimethylformamide ≥ 99.99995% 1 L 1. Michalke, B. The coupling of LC to ICP-MS in element speciation; Part II: Recent trends in application. Trends in Analytical Chemistry, 2002, 21(3), 154–165. 2. IUPAC, Pure and Applied Chemistry, 2000, 72, 1453–1470. The International Union of Pure and Applied Chemistry (IUPAC) defines speciation analysis as the “analytical activities of identifying and/or measuring the quantities of one or more individual chemical species in a sample.” A chemical species is a “specific form of an element defined as to isotopic composition, electronic or oxidation state, and/or complex or molecular structure.” In speciation analysis, the objective is usually to determine the identity and/or concentration of one or more chemical species in a sample, often of natural origin and therefore potentially containing many different species. Care must be taken to choose and execute the analysis to maximize sensitivity and specificity. Performing a speciation analysis in a complex mixture involves separation, identification, and characterization of various forms of elements in the sample. The most commonly used strategy for speciation analysis is to perform a separation step before a generic detector. LC separation coupled with ICP-MS The hyphenated technique HPLC-ICP-MS is a robust, sensitive element-selective method capable of giving a complete picture of the elemental species in a solution. The elemental response is usually independent of species, so it is often possible to quantify a species even when its structure is unknown (assuming good HPLC recovery). Identification, however, is based solely on retention time matching. Therefore, a compound in the sample can be identified only by comparison with a standard. Definition of Speciation Analysis Honeywell Riedel de Haën - 2-Propanol TraceSELECT 04516-1L

Product Number Brand Product Name Specification Packaging 94068 Fluka Citric acid monohydrate ≥99.9998% 100 g 05878 Fluka L-Ascorbic acid ≥99.9998% 100 g 12819 Fluka DL-Tartaric acid ≥99.9995% 25 g 39692 Fluka Ethylenediaminetetraacetic acid ≥99.995% 25 g 93722 Fluka Oxalic acid dihydrate ≥99.9999% 100 g High Purity Reagents for Voltammetry 9 10 TraceSELECT reagents for ultra-trace analysis and metal speciation Voltammetry is predominantly used for inorganic trace analysis of anions and cations, but can also be used for the determination of various organic compounds. The most important fields of application of inorganic determinations are in metallurgy, environmental analysis, food analysis, toxicology, and clinical analysis. This technique is also a preferred method for the determination of certain metal speciations, such as Fe(II)/Fe(III) or Cr(III)/Cr(VI). When mercury is used as an electrode in a voltammetric cell, the technique is called polarography. The reduction of metals into mercury is more favourable than reduction to the solid-state electrode. Further on there is always a clean new Hg electrode surface available for each measurement. Ultra Pure Reagents for Voltammetry Multi-mode Electrode (MME) Published with kind permission of Metrohm AG, Switzerland Honeywell Fluka - Nitric Acid TraceSELECT 84385-2.5L

All statements and information provided herein are believed to be accurate and reliable, but are presented without guarantee, warranty or responsibi lity of any kind , express or impl ied. Statements or suggestions concerning possible use of our products are made without representation or warranty that any such use is free of patent infringement, and are not recommendations to infringe any patent. The user should not assume that all safety measures are indicated herei n, or that other measures may not be required. User assumes all liability for use of the information and results obtained. Fluka and TraceSELECT are trademarks of Hon eywell Sp ecialty Chemicals Seelze GmbH. NPI_001_TRACE_ENG | 05/17 v_12 © 2017 Honeywell International Inc. All rights reserved. Honeywell Specialty Chemicals Seelze GmbH Wunstorfer Strasse 40 D-30926 Seelze, Deutschland www.honeywell.com To order, please contact: APEX CHEMICALS CO., LTD. (Head Office) 2034/87, Italthai Tower 19th Floor, New Petchburi Road, Bangkapi, Huaykwang, Bangkok 10310, Thailand Tel. 02-038-9999 Fax. 02-038-0099 @ [email protected] f ApexChemicals Line @ApexChemicals www.apexchemicals.co.th

Research Chemicals Inorganic Trace Analysis TraceSELECT™ High Purity Reagents for Sample Preparation and Analysis Discover the perfect formula TraceSELECTTM Honeywell Highest purity Reliability Ts + Hw = Py2 + Ry

Sample Preparation for Trace Analysis: TraceSELECT™ Ultra and TraceSELECT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Trace Analysis: High Purity Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Solvents for Trace Metal Speciation Analysis: TraceSELECT solvents for LC-ICP-MS applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Definition of Speciation Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Ultra Pure Reagents for Voltammetry: TraceSELECT reagents for ultra-trace analysis and metal speciation . . . . . . . . . . . . . 10 Contents

1. Feng, X.; Wu, S.; Wharmby, A.; Wittmeier, A. Microwave digestion of plant and grain standard reference materials in nitric and hydrofluoric acids for multi-elemental determination by inductively coupled plasma mass spectrometry. J. Anal. At. Spectrom. 1999, 14, 939–946. 2. Golimowski J.; Golimowska, K. UV photooxidation as pretreatment step in inorganic analysis of environmental samples. Anal. Chim. Acta. 1996, 325, 111–133. 3. Vandecasteele, C.; Block, C. B. Modern Methods for Trace Element Determination; Wiley: Chichester, UK, 1997; pp 1–8. 4. Sampling and Sample Preparation; Stoeppler, M., Ed; Springer: Berlin, 1997. 5. Sah, R. N.; Miller, R. O. Spontaneous reaction for acid dissolution of biological tissues in closed vessels. Anal. Chem. 1992, 64, 230. 6. Inductively coupled plasmas in analytical atomic spectrometry; Montaser, A.; Golightly, D. W., Eds.; VCH: New York, 1992; pp 473–516. 7. Tracy, M. L.; Moeller, G. Continuous flow vapor generation for inductively coupled argon plasma spectrometric analysis; Part 1: Selenium. J. Assoc. Off. Anal. Chem. 1990, 73, 404–410. 8. Pacquette, H. L.; Elwood, S. A.; Ezer, M.; Simeonsson, J. B. A comparison of continuous flow hydride generation laser-induced fluorescence and laser enhanced ionization spectrometry approaches for parts per trillion level measurements of arsenic, selenium and antimony. J. Anal. At. Spectrom. 2001, 16, 152–158. 9. Sun, Y. C.; et al. Combining electrothermal vaporization inductively coupled plasma mass spectrometry with in situ TMAH thermochemolysis for the direct determination of trace impurities in a polymer-based photoresist. J. Anal. At. Spectrom. 2006, 21, 311–316. 10. Jeitziner, M. High-purity TMAH for alkaline digestion of biological samples. Analytix 2008, 1, 17. 11. Jeitziner, M. High-Purity Reagents for AAS. Analytix 2011, 1, 14. Reagents for Inorganic Trace Analysis Analysis you can trust 2 Sensitive trace analysis applications require extremely pure sample preparation reagents. These digestive reagents cannot contain metal ions or other impurities. In addition, complete decomposition of the sample is required to achieve reproducible and accurate elemental results by instrumental analytical methods. Sample wet digestion/dissolution is a method that breaks down the components of a matrix into simple chemical forms. This digestion can occur in three ways: w With the introduction of energy such as heat w By using a chemical reagent such as an acid w By a combination of these two methods Most analytical measurements using highly sensitive methods (AAS, ICP-AES, stripping voltammetry, ion chromatography, etc.) are performed on samples in solution. One of the most effective and economical sample preparation methods is microwave digestion. In most cases, achieving homogeneity and mineralization of the sample is sufficient. UV photolysis using hydrogen peroxide and either potassium persulfate or nitric acid is very often the method of choice for the decomposition of organic impurities in aqueous solutions. The most commonly used reagents for wet decomposition are mineral and oxidizing acids. Wet decomposition has the advantage of being effective on both inorganic and organic materials. It often destroys or removes the sample matrix, thus helping to reduce or eliminate some types of interference. Table 1 provides an annotated overview of the acids and bases used for wet digestion. With Honeywell’s analytical reagents, superior quality and safety standards are a prime focus. We use a comprehensive Quality Assurance System in line with EN 29001 (ISO 9001) and each of our products is manufactured to clear, guaranteed specifications. We employ a large variety of modern analytical methods, such as inductively coupled plasma atomic emission spectroscopy (ICP-OES), inductively coupled plasma mass spectrometry (ICP-MS) , flame atomic absorption spectroscopy (AAS) and graphite furnace AAS. This allows for specifically tailored control and analysis procedures for each product. Sources Atomic Emission Spectroscopy Analysis

3 HNO3 Nitric acid is the most frequently utilized sample dissolution medium. It oxidizes metals not dissolved by HCl and other non-oxidizing acids. Au, Pt metals (except Pd), Nb, Ta, and Zr are not dissolved. Al and Cr are passivated. Sn, Sb, and W give insoluble hydrous oxides. Dissolves most sulfides (except HgS). Unfortunately, the carbon contained in organic materials is only partly converted to CO2 by HNO3 at temperatures up to 200 °C. Nitric acid should never be used for the digestion of highly aromatic compounds because of the potential for the formation of highly explosive compounds. In the case of alcohols, the samples should be pretreated with sulfuric acid. HCl Hydrochloric acid is used for many salts of weak acids, e.g., carbonates, phosphates, some oxides, and some sulfides. H2SO4 Sulfuric acid is used when its high boiling point (300 °C) is an advantage, as in expelling a volatile product or increasing the reaction rate. It provides dehydrating and oxidizing properties at high temperatures. HClO4 Perchloric acid is a very powerful oxidizing agent at fuming temperatures (boiling point 203 °C). It is usually mixed with HNO3 to oxidize easily attacked organic matter that might otherwise react violently with HClO4. H2SO4 (dehydrating agent) increases oxidizing power. Good solvent for stainless steel and for sulfides. HF Hydrofluoric acid is used for digestion of silacaceous samples and as an auxiliary reagent to HNO3 or HClO4 to eliminate fluoride. With HNO3, HF dissolves Ti, W, Nb, and Zr (and their carbides, nitrides, and borides) as a result of formation of complex fluorides. Certain refractory silicates and other minerals are not decomposed; these must be dissolved by fusion. H2O2 Hydrogen peroxide is a very popular oxidizing reagent as it is converted to water and oxygen during the oxidation of biological material. Additional advantages are that there is no acid corrosion of the digestion vessel PTFE walls, no formation of insoluble salts with an acid anion, and no change of the sample matrix by an acid. Because of its strong oxidation power, only small amounts of H2O2 need be used so concentrated sample solutions can be obtained. AcOH Acetic acid is most often used for the extraction of metallic impurities together with sodium acetate. HBr Hydrobromic acid is a non-oxidizing acid. It is used in some special applications because it yields better reductive selectivity in the determination of Se(VI) by Hydride Generation-Laser Induced Fluorescence (HG-LIF) than HCl. H3PO4 Phosphoric acid is commonly used in the semiconductor industry to both clean and etch metal surfaces. The concentration of the phosphoric acid is critical to optimizing these processes. H2O Water ensures the highest accuracy in trace analysis of ppt range by minimizing blank values. TMAH Tetramethylammonium hydroxide [(CH3)4NOH] is an efficient strong alkali used for solubilizing soft tissues and food stuffs. Compared to conventional alkaline sample digestion procedures using NaOH or KOH, using TMAH enables more accurate analysis due to its lower matrix effect. A diluted 0.3 M TMAH solution is also used during the lithographic process in the semiconductor industry. NaOH Sodium hydroxide is frequently used for melting digestions at 500 °C. It attacks platinum and porcelain. Residue is dissolved with 12 M hydrochloric acid. KOH Potassium hydroxide is frequently used for melting digestions at 500 °C. It attacks platinum and porcelain. Residue is dissolved with 12 M hydrochloric acid. K2CO3 Potassium carbonate is the classic reagent for melting digestions at 800 °C. Residue is dissolved with hydrochloric acid. Digestion/Dissolution Acids and Bases Table 1

4 Sample Preparation for Trace Analysis TraceSELECT™ Ultra and TraceSELECT Sample preparation for trace analysis requires reagents of the highest purity. Our TraceSELECT Ultra products for ultra-trace analysis at ppb and even ppt levels are produced by sub-boiling distillation. Sub-boiling is recognized as the best way to obtain high purity acids with the lowest blank values for ultra-trace analysis. The technique is based on the evaporation of liquid by infrared heating at the surface. It avoids violent boiling and the formation of liquid aerosols that can be transported with the distillate. To maintain their high purity, TraceSELECT Ultra products are supplied in PTFE PFA (fluoropolymer) bottles. Water and ortho-phosphoric acid are supplied in especially pre-leached HDPE bottles. Recent process improvements have allowed us to reduce our impurity specifications to guarantee the lowest levels of trace impurities in our TraceSELECT Ultra products. The acids, bases, and salts in the TraceSELECT series have been developed for sample preparation and analysis in the ppb (μg/kg) trace range. Purity and composition are guaranteed with our careful preparation, testing, and verification of the final product for metal content and ionic trace impurities using ICP-OES, ICP-MS and ion chromatography. To further guarantee purity and stability, TraceSELECT products are packaged in high-quality containers appropriate for each product. The Honeywell Quality Management System guarantees consistent quality and safety for all TraceSELECT Ultra and TraceSELECT products. The reagents are produced and bottled under clean-room conditions. For more information, visit: lab-honeywell.com/traceselect Ultra-pure acids, bases, and salts for smelting and wet digestion in environmental, water, and food analysis. Honeywell Fluka - Ammonium Acetate TraceSELECT 73432-100G

5 Product Number Brand Product Name Specification Packaging 45727 Fluka Acetic acid ≥ 99.0% (T) 100 mL, 500 mL ,1 L, 2.5 L 09857 Fluka Ammonium hydroxide solution ≥ 25% in water (T) 100 mL, 500 mL 06454 Fluka Formic acid ≥ 88.0% (T) 250 mL 08256 Fluka Hydrochloric acid ≥ 30% (T) 100 mL, 500 mL , 1 L, 2.5L 84415 Fluka Hydrochloric acid ≥ 37% fuming (T) 100 mL, 500 mL 47559 Fluka Hydrofluoric acid 47-51% (AT) 100 mL, 500 mL 84385 Fluka Nitric acid > 69.0% (T) 250 mL, 500 mL, 1 L, 2.5L 77227 Fluka Perchloric acid 67-72% (T) 100 mL, 500 mL, 1 L 79614 Fluka Phosphoric acid ~ 85% (T) 100 mL, 500 mL 30955 Fluka Potassium hydroxide solution ≥ 30% in water (T) 250 mL 13171 Fluka Sodium hydroxide solution ≥ 30% in water (T) 250 mL 84716 Fluka Sulfuric acid ≥ 95% (T) 500 mL, 1 L, 2.5 L 68556 Fluka Tetramethylammonium hydroxide solution 25% in water 250 mL 95305 Fluka Water 250 mL, 500 mL, 1 L, 2.5 L, 10 L TraceSELECT Reagents Product Number Brand Product Name Specification Packaging 07692 Fluka Acetic acid >99.0% (T) 250 mL, 500 mL, 1 L 96208 Fluka Hydrochloric acid >30-35% 250 mL, 500 mL, 1 L 02650 Fluka Nitric acid >65-71% 250 mL, 500 mL, 1 L, 2 L 12415 Fluka Perchloric acid 67-72% (T) 250 mL, 500 mL, 1 L 64957 Fluka Phosphoric acid >85% (T) 250 mL, 1 L 77239 Fluka Sulfuric acid >95% (T) 250 mL, 500 mL, 1 L 14213 Fluka Tetramethylammonium hydroxide solution 25% in water 250 mL TraceSELECT Ultra Reagents Product Number Brand Product Name Specification Packaging 73432 Fluka Ammonium acetate ≥ 99.9995% 100 g 09725 Fluka Ammonium chloride ≥ 99.9995% 25 g, 100 g 09726 Fluka Ammonium phosphate monobasic ≥ 99.9999% 25 g, 100 g 09979 Fluka Ammonium sulfate ≥ 99.9999% 100 g 90033 Fluka Cesium chloride ≥ 99.9995% 25 g, 100 g 16722 Fluka Cesium iodide ≥ 99.9995% 25 g 62462 Fluka Lithium carbonate ≥ 99.998% 25 g, 100 g 60348 Fluka Potassium bisulfate ≥ 99.995% 25 g 60111 Fluka Potassium carbonate sesquihydrate ≥ 99.995% 50g TraceSELECT Salts

Matrix Modifiers In graphite furnace AAS, element determinations are increasingly being carried out with matrix modifiers. Chemical modification should be considered if an analyte is highly volatile, or if the analyte and matrix volatilize at similar temperatures. Such modification would allow ashing at higher (or atomization at lower) furnace temperatures, resulting in elimination of the matrix with no loss of the analyte (or atomization of the analyte, but not the matrix). Depending on the element to be determined, various substances are used. However, palladium nitrate has become one of the most popular. A primary criterion for such substances is the absence of the element to be analyzed. For this reason, the following recommended reagents have been specially tested for their suitability as matrix modifiers. Trace Analysis: High Purity Reagents Product Number Brand Product Name Specification Packaging 95164 Fluka Cesium chloride/Lanthanum chloride matrix modifier solution CsCl and LaCl3 250 mL, 500 mL, 1 L 428868 Fluka Calcium matrix modifier solution 2% Ca²+ in 1% HNO3 100 mL 428884 Fluka Lanthanum matrix modifier solution 5% La³+ in 1% HCl 100 mL 428892 Fluka Magnesium matrix modifier solution 2% Mg²+ in <5% HNO3 100 mL Matrix Modifiers 6 Melting digestion is used for solid samples like ores, rock, metals, alloys, ceramic, and cement, in order to obtain a homogenous residue, which can be dissolved in diluted TraceSELECT acids. Our TraceSELECT salts obtain a very high purity, with metal traces typically below 10 μg/kg (10 ppb). TraceSELECT Salts (continued) Product Number Brand Product Name Specification Packaging 05257 Fluka Potassium chloride ≥ 99.9995% 25 g, 100 g 60216 Fluka Potassium phosphate monobasic ≥ 99.995% 25 g, 100 g 60371 Fluka Potassium hydroxide hydrate ≥ 99.995% 25 g 30533 Fluka Potassium iodide ≥ 99.999% 100 g 60347 Fluka Potassium phosphate dibasic ≥ 99.999% 100 g 60429 Fluka Potassium nitrate ≥ 99.995% 25 g, 100 g 59929 Fluka Sodium acetate ≥ 99.999% 25 g, 100 g 71347 Fluka Sodium carbonate ≥ 99.9999% 25 g, 100 g 38979 Fluka Sodium chloride ≥ 99.999% 25 g, 100 g 01968 Fluka Sodium hydroxide monohydrate ≥ 99.9995% 25 g, 100 g 71752 Fluka Sodium nitrate ≥ 99.999% 25 g, 100 g 71629 Fluka Sodium phosphate dibasic ≥ 99.999% 100 g 71492 Fluka Sodium phosphate monobasic ≥ 99.999% 25 g, 100 g

7 Spectroscopic Buffers for Flame AAS In flame AAS, spectroscopic buffers are often used to suppress physical, ionization, and chemical interferences. The Schuhknecht and Schinkel buffer solution, as used in the determination of alkali elements, has become particularly important. A multi-element standard for lithium, sodium, and potassium is also available for this method. The Schinkel buffer solution enables the method to be expanded to include up to 14 mono- and divalent elements, with simple calibration being all that is required for the analysis. Reducing Agents for Hydride AAS Hydride AAS is used for the analysis (especially traces) of arsenic, antimony, tin, selenium, bismuth, and mercury. It is used to separate and preconcentrate analytes from sample matrices by a reaction that turns them into their hydride vapors. Sodium borohydride is the common reagent of choice for the reduction. Fluka reagents are specifically analyzed to ensure the absence of hydride generating metals. Product Number Brand Product Name Specification Packaging 20980 Fluka Aluminum nitrate-Cesium chloride buffer Buffer solution according to Schuhknecht and Schinkel 500 mL 20982 Fluka Cesium chloride-Lanthanum chloride buffer Buffer solution according to Schinkel 500 mL 95164 Fluka Cesium chloride-Lanthanum chloride buffer CsCl 100 g/L, LaCl3 100 g/L (in water) 250 mL Spectroscopic Buffers for Flame AAS 1. Schuhknecht, W.; Schinkel, H. Fresenius J. Anal. Chem. 1963, 161, 194. 2. Schinkel, H. Fresenius J. Anal. Chem. 1984, 10, 317. Product Number Brand Product Name Specification Packaging 71321 Fluka Sodium borohydride ≥ 99% 25 g, 100 g 30533 Fluka Potassium iodide ≥ 99.999% 100 g Reducing Agents for Hydride AAS

Min 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 8 Honeywell has developed high purity TraceSELECT solvents for speciation analysis by LC-ICP-MS. TraceSELECT solvents undergo rigorous purification procedures. This is followed by UV spectroscopy, IC, and ICP-MS testing to assure high chemical purity and high UV transmittance. The blank values for metal traces in these solvents are in the ppb range or lower. The toxicity of trace metals in food, clinical chemistry, biology, and environmental sciences is an area of increasing interest. In the environment, many diverse species of an element can be present. In addition, different species of the same element can possess very different degrees of toxicity. Monitoring elemental species requires an analytical method that is sensitive and specific enough to resolve and quantify the individual species at ultratrace levels. An individual toxic species may constitute only a fraction of an element’s total concentration in a sample. The coupling of the two well established analytical techniques, HPLC and inductively coupled plasmamass spectrometry (ICP-MS), is straightforward. This is primarily because the flow rates commonly used with LC are compatible to conventional liquid sample introduction systems, such as those based on pneumatic nebulization. Thus, the outlet of the LC column is directly connected to the ICP-MS nebulizer. This technique is especially useful in carrying out automated highthroughput speciation analysis. International trends show speciation of metals as an area of very high research interest. In the future, speciation of biological samples will undoubtedly involve determinations of which organic molecules attract which metals in specific sample types. ICP-MS is a superior detection technique for trace elements in general, but especially for elements of interest, such as arsenic, selenium, cadmium, iodine, and others in chromatographic eluents. TraceSELECT™ solvents for LC-ICP-MS applications Solvents for Trace Metal Speciation Analysis The chromatogram of three mercury species Hg2+, MeHg+ , EtHg+ obtained by HPLC-ICP-MS