Abstractbook SCIfair 2021_No3

Preface

As John Holt, American teacher and writer once said Learning is
not the product of teaching. Learning is the product of the activity of learners.
Our Faculty of Science at Srinakharinwirot University likewise strives to
provide the greatest science education possible, and in order to do so, learners'
participation, particularly in research, is critical.

The seventh SCI Fair-Senior Project Presentation for 2021 allows
Bachelor degree students to share their scientific research, educational experiences,
and network with other participants. This year, we have participants from 13
curricular and 155 research projects. All of the research processes were the
result of students' hard work and perseverance, as well as the mentors who
guided and mandated students to complete their senior projects. Furthermore, in
order to improve students' soft skills in English communication, both writing
and speaking, students were encouraged to write an abstract for their seniors
project and present their works in the form of a three-minute thesis.

I would like to thank all of the students and faculty members at the
Faculty of Science for their dedication and management of this project. They
have put forth a lot of effort reviewing papers and offering helpful comments to
students on how to enhance their works. Finally, we hope that everyone will
find this abstract book to be a useful resource for future research.

Prof. Parin Chaivisuthangkura, Ph.D., SFHEA

Dean, Faculty of Science,

Srinakharinwirot University

Program Committee

Advisor Committee

Prof. Dr.Parin Chaivisuthangkura
(Dean of the Faculty of Science)
Asst. Prof. Dr.Nalena Praphairaksit
(Associate Dean for Academic Affairs and International Relations)
Assoc. Prof. Dr.Apinya Chaivisuthangkura
Assoc. Prof. Dr.Siritron Samosorn

Organization Committee Assoc. Prof. Dr.Nattahapong Phinichka
Asst. Prof. Dr.Maneekarn Namsa-aid
Asst. Prof. Dr.Siri Sirininlakul Asst. Prof. Dr.Surawut Wicharn
Asst. Prof. Dr.Chantri Polprasert Asst. Prof. Dr.Nissara Sirasuntorn
Assoc. Prof. Dr.Kriangsak Songsrirote Asst. Prof. Dr.Thitarie Rungratgasame
Asst. Prof. Dr.Puenisara Limnonthakul Dr.Nopnithi Thonghin
Asst. Prof. Dr.Thapana Chontananarth Dr.Thanit Siriboon
Dr.Kulvadee Dolsophon Mrs.Vanida Pasipol
Dr.Pornthip Boonsri
Ms. Yupawadee Kosa
Mr.Chanachai Thongyoo

Abstract Publication Committee

Assoc. Prof. Dr.Achariya Rangsiruji Assoc. Prof. Dr.Apinya Chaivisuthangkura

Assoc. Prof. Dr.Kriangsak Songsrirote Assoc. Prof. Dr.Nattahapong Phinichka

Assoc. Prof. Dr.Ratchanok Thongnum Assoc. Prof. Dr.Siritron Samosorn

Asst. Prof. Dr.Akarin Boonsombuth Asst. Prof. Dr.Areeya Aeimbhu

Asst. Prof. Dr.Chira Lumduanhom Asst. Prof. Dr.Nuttika Suwannasai

Asst. Prof. Dr.Panyawat Ha-ar-sa Asst. Prof. Dr.Patamas Bintachitt

Asst. Prof. Dr.Pisuttawan Sripirom Sirininlakul Asst. Prof. Dr.Prapakorn Tantayotai

Asst. Prof. Dr.Puenisara Limnonthakul Asst. Prof. Dr.Sasivimon Sukaphat

Asst. Prof. Dr.Sirinun Kaenthong Asst. Prof. Dr.Sirisup Laohakiat
Asst. Prof. Dr.Somkiat Phornphisutthimas Asst. Prof. Dr.Suchao Donpudsa
Asst. Prof. Dr.Sukhumaporn Krajangsang Asst. Prof. Dr.Supphachai Thaicharoen
Asst. Prof. Dr.Suwan Plaipichit Asst. Prof. Dr.Thanawan Tejangkura
Asst. Prof. Dr.Wasutep Luangtip Dr.Jamaree Amonkosolpan
Dr.Jitsopa Chaliewsak Dr.Kongkeat Jampasri
Dr.Jittinart Rattanamoong Dr.Karnchana Panichakarn
Dr.Natee Ampai Dr.Nopmanee Supanam
Dr.Pirapan Polburee Dr.Ponlkrit Yeesin
Dr.Prawat Aungpraphapornchai Dr.Suchada Pongprasert
Dr.Sukanya Intarapak Dr.Sukanya Suksak
Dr.Supakan Ratanakon Dr.Thitirat Charoonsuk
Dr.Uthoomporn Mato Dr.Weena Thongrod
Dr.Wiphusinee Worrachottiyanon Ms. Montawan Chorchomkasem
Mr.Kiattisak Phutamee Mrs.Vanida Pasipol

Management Presentation Committee

Asst. Prof. Dr.Nalena Praphairaksit Asst. Prof. Dr.Chaninan Pruekpramool
Asst. Prof. Dr.Puenisara Limnonthakul Asst. Prof. Dr.Nissara Sirasuntorn
Asst. Prof. Dr.Surawut Wicharn Asst. Prof. Dr.Suwan Plaipichit
Asst. Prof. Dr.Thapana Chontananarth Asst. Prof. Dr.Thitarie Rungratgasame
Dr.Thanit Siriboon Ms. Yupawadee Kosa
Mrs.Vanida Pasipol Mr.Chanachai Thongyoo

Oral Presentation Committee Assoc. Prof. Dr.Onanong Pringsulaka
Assoc. Prof. Dr.Siwaporn Longyant
Assoc. Prof. Dr.Numphon Koocharoenpisal Asst. Prof. Dr.Anitthan Srinual
Assoc. Prof. Dr.Pichapak Sriyapai Asst. Prof. Dr.Charttirot Karaveg
Assoc. Prof. Dr.Walaikorn Nitayaphat Asst. Prof. Dr.Nuttapon Apiratikul
Asst. Prof. Dr.Anusit Thongnum
Asst. Prof. Dr.Nahathai Rerkruthairat

Asst. Prof. Dr.Panarat Arunrattiyakorn Asst. Prof. Dr.Panitarn Wanakamol
Asst. Prof. Dr.Rakchanok Koto Asst. Prof. Dr.Rossaporn Jiamjariyatam
Asst. Prof. Dr.Ruangvarin Sararnrakskul Asst. Prof. Dr.Siriruk Sarawareeyaruk
Asst. Prof. Dr.Songsak Phonghirun Asst. Prof. Dr.Suphot Musiri
Asst. Prof. Dr.Supitcha Supansomboon Asst. Prof. Dr.Thunyakorn Chuaytukpuan
Asst. Prof. Dr.Yanin Kongthip Dr.Angkana Kokaew
Dr.Apirada Sathapattayanon Dr.Atchariya Watthanawisut
Dr.Bongkoj Boonburapong Dr.Itthithep Navarasuchitr
Dr.Kem Pumsa-Ard Dr.Kulvadee Dolsophon
Dr.Manoch Hengwattana Dr.Montree Maneepark
Dr.Napa Sae-Bae Dr.Nopnithi Thonghin
Dr.Phieraya Pulphol Dr.Piyapong Sitthison
Dr.Pornthip Boonsri Dr.Ruangsak Trakunphutthirak
Dr.Sophon Mongkolluksamee Dr.Subhorn Khonthapagdee
Dr.Suthamat Niyompanich Dr.Teerasak Chaladgarn
Dr.Thanit Siriboon Dr.Thunwa Theerakarn
Dr.Wanlapa Lorliam Dr.Witsarut Pho-On
Dr.Wittaya Pakum Dr.Wuttipong Tongbai

Program Schedule SCIFAIR 2021

Important Date Period
February – 15 March 2022
Detail 17 March – 17 April 2022
Abstract Registration 20 April – 25 April 2022
Abstract Submission 25 April – 29 April 2022
Abstract Acceptance 25 April – 30 April 2022
Abstract Full Paper Submission
Oral Presentation by VDO Clip 17 May 2022
SCIFAIR 2021 Conference

May 17, 2022, Room 19-903, Building 19, Faculty of Science, SWU

Time Detail
08.30 A.M – 09.00 A.M Registration
Opening Ceremony by
09.00 A.M – 09.15 A.M Dean of the Faculty of Science
Prof. Dr.Parin Chaivisuthangkura

Meeting ID: 977 5203 2234
Passcode: 209310

09.15 A.M – 12.00 A.M Announcement of Presentation Award
12.00 A.M – 12.15 A.M. Close Ceremony

Table of Contents

Abstract Page

1. Bachelor of Science Program in Mathematics 1
2
MATH01 Transposition and inversion on Fibonacci numbers 3
4
MATH02 On the sums of reciprocal Lucas numbers

MATH03 Dividing the perimeter of a triangle into unequal proportions

MATH04 On solutions to the Diophantine equations and

MATH05 On nilpotent matrices satisfying a binomial-like matrix equation 5
MATH06 Measuring political gerrymandering by district average wasted votes 6
MATH07 The gambling dice game made fair 7
MATH08 Magic cubes and linear algebra 8
MATH09 Face colorings of triangular bipyramids and cubes with colors 9
The edge-sum game 10
MATH10
11
2. Bachelor of Science Program in Statistics
12
STAT01 Prediction of the number of Covid-19 confirmed cases in Thailand by 13
Box-Jenkins and Holt's exponential smoothing methods
14
STAT02 Factors affecting fertility rate of Thailand 15

STAT03 Factors influencing the drug offense cases rate in Thailand in the year 16
2020
17
STAT04 Depression structural equation model with empirical data

STAT05 Factors affecting satisfaction of students toward the Faculty of Science,
Srinakharinwirot University

STAT06 Forecasting model for the number of deceased from road accidents in
Thailand

STAT07 A study on factors affecting online football gambling behaviors of
undergraduate students Srinakharinwirot University

Abstract Table of Contents Page
STAT08 18
Forecasting model of the closing price of Bangkok Life Assurance
Public Company Limited

3. Bachelor of Science Program in Chemistry

CHEM01 Development of a high-performance liquid chromatography method 19
for determination of mangiferin 20
21
CHEM02 The synthesis Carbon Dots from mangosteen peel as a fluorescent 22
probe for irons determination

CHEM04 Development of colorimetric method for the measurement of cysteine

CHEM05 Design of novel coumarin derivatives as angiotensin converting enzyme
inhibitor

4. Bachelor of Science Program in Biology

BIO01 Effects of zinc sulfate on the production of lutein and canthaxanthin 23
in Chlorella zofingiensis 24
25
BIO02 Leaf blade, petiole and wood anatomy of some species in the genus 26
Annona and Goniothalamus (tribe Annoneae (Annonaceae)) in Thailand 27
28
BIO03 Effect of drought stress on photosynthesis efficiency and the quantity 29
of bioactive compounds of Mentha cordifolia Opiz ex Fresen 30
31
BIO04 PCR-based detection of hepatotoxic mushrooms in the genus Amanita

BIO05 Effect of noise exposure on cognitive performance in college students

BIO06 Identification of metabolic toxin-containing mushrooms based on
molecular techniques

BIO07 Isolation of biosurfactant-producing bacteria from oil wastewater in
Bangkok

BIO08 Characterization and expression analysis of Interferon regulatory
factor (IRF) gene from Macrobrachium rosenbergii

BIO09 Characterization and expression analysis of Toll interacting protein
(Tollip) from Macrobrachium rosenbergii

Abstract Table of Contents Page
BIO10 32
BIO11 Production of polyclonal antibodies against recombinant S8 structural 33
BIO12 protein of Tilapia lake virus (TiLV) 34
BIO13 Generation of polyclonal antibodies against Salmonella enterica 35
serovar Virchow and S. enterica serovar Hadar
Detection of a fish pathogen Aeromonas veronii using polyclonal
antibodies
Influence of culture medium on in vitro seed germination and seedling
development of orchids

5. Bachelor of Science Program in Microbiology

MICRO01 Antimicrobial activities of fungal crude extracts obtained from 36
mycelium and culture broth 37
38
MICRO02 Antimicrobial susceptibility and genetic characterization of 39
Enterobacteriaceae isolated from environment and food samples 40
41
MICRO03 Kombucha beverage from coffee cherry pulp tea (cascara tea) 42
43
MICRO04 Inhibition of Staphylococcus aureus in household materials by culture 44
filtrate of Pseudomonas aeruginosa SWUC02 45

MICRO05 Biodegradable plastic degradation by co-culture of Actinomadura
keratinilytica (T16-1) and Laceyella sacchari (LP175) in shake flask

MICRO06 Optimization condition for Bacillus subtilis strain OK1101 production
for the elimination of weedy rice

MICRO07 Mechanism of DNA repair by homologous recombination

MICRO08 Identify and select psychrotolerant yeast for use as starter cultures in
MICRO09 the fermentation step of wet processing

An in vitro study of neuropathogenic fungi in neuroblastoma cells
(SH-SY5Y)

MICRO10 Scale-up of Pichia kluyveri YML1-1 production for arabica coffee
fermentation in airlift fermenter

Table of Contents

Abstract Page
46
MICRO11 Biosafety investigation of Wickerhamomyces anomalus YWP1 -3 for 47
MICRO12 use as starter culture in Arabica coffee production 48
MICRO13 49
MICRO14 Distribution of CD28 gene polymorphisms in Thai population 50
MICRO15 51
MICRO16 Correlation of medically important parasites and cockroach species 52
MICRO17 diversity in Srinakharinwirot University 53
MICRO18 54
MICRO19 Co-culture of Trichoderma asperellum and PGPR for enhancing the 55
MICRO20 antifungal activity against Pyricularia oryzae 56
MICRO21 57
MICRO22 Optimization of seed culture production for arabica coffee fermentation 58
MICRO23 in shake flask 59
MICRO24 60
MICRO25 Determination of root exudates factor affecting the inhibition of weedy 61
MICRO26 rice growth 62
MICRO27
Screening of probiotics for the development of honey jelly gummy
formulation

Screening of oleaginous yeast for lipid production using agro-industrial
waste

Screening of psychrophilic yeasts for improvement of arabica coffee
quality

Development of dog snack impregnated with probiotic bacteria

Detection of antibodies to enterovirus 71 and coxsackievirus A16 in
adult serum by virus neutralization assay

Determination of Leptospira spp. concentration for microscopic
agglutination test (MAT)

The selection of oleaginous yeasts for lipid production using sodium
acetate as a carbon source

Isolation and characterization of bacteriophages for controlling
Pseudomonas in milk

The diversity of yeasts isolated from flowers in Thailand

Decolorization of industrial effluent by white-rot fungus Microporus
xanthopus PP17-17 and ligninolytic enzyme study

Fungal pigment extraction for natural dyeing of cotton fabric

Table of Contents

Abstract Page
63
MICRO28 Amazake production from different varieties of Thai rice and their 64
MICRO29 antioxidant activities
MICRO30 65
MICRO31 Pathogenicity of Alternaria sp. on Capsicum annuum cv. ‘yellow pepper’ 66
MICRO32 and controlling of the pathogen by cell-free culture of Pseudomonas
aeruginosa SWUC02 67

The prevalence of Mycobacterium abscessus in Phramongkutklao
Hospital

Pathogenicity of Colletotrichum truncatum causing anthracnose disease
on Capsicum cultivars and controlling of the pathogen by cell-free
culture of Pseudomonas aeruginosa SWUC02

Development of a healthy drink from cashew apple (Anacardium
occidentale L.)

6. Bachelor of Science Program in Physics

PHY01 Synthesis and characterization of graphene oxide-titanium dioxide 68
nanocomposites 69
70
PHY02 Study of titanium dioxide nanotubes coated with gold particles 71
72
PHY03 The preparation of TiN nanorods by magnetron sputtering for surface- 73
enhanced Raman scattering 74
75
PHY04 The study of properties of cotton fabric modified with titanium dioxide
PHY05 coatings

A prototype development of triboelectric – electromagnetic hybrid
nanogenerator

PHY06 Nonlinear model selection for accurate monthly global solar radiation
prediction in Bangkok, Thailand

PHY07 Estimating global solar radiation from common meteorological data
in Prachinburi, Thailand

PHY08 Simple smart environmental signage for air pollution monitoring in
urban residential areas

Table of Contents

Abstract Page

7. Bachelor of Science Program in Materials Science 76
77
MAT01 Effect of gum additives on nickel electroplating 78
79
MAT02 Effect of inorganic dyes on the charge controlling and output efficiency
of the cotton-based triboelectric nanogenerator 80
81
MAT03 Phosphorescence and tarnish resistance properties of copper with 82
strontium aluminate addition
83
MAT04 Enhancing the output efficiency of Polydimethylsiloxane by adding 84
85
colossal dielectric materials for flexible triboelectric nanogenerator 86
(F-TENG) 87

MAT05 Bacterial cellulose-based triboelectric nanogenerator enhanced
performance with two-dimensional dielectric oxide nanosheet

MAT06 Triboelectric-piezoelectric hybrid nanogenerator based on natural
polymer/gamma-glycine composites

MAT07 The production of bacterial cellulose skin masks synthesized by
Komagataeibacter nataicola TISTR 975 bacteria using coffee grounds
as a culture medium

MAT08 Influence of tannin from Indian almond leaves on the curing behavior
of sucrose-citric acid adhesive

MAT09 Production of binderless particleboard from rice straw mixed with
banana pseudostem

MAT10 The influence of supplements on the production of bacterial cellulose
by Komagataeibacter nataicola TISTR 975

MAT11 The production of particleboard from oil palm fiber using lignin
formaldehyde synthesized from oil palm kernel shell

MAT12 Fabrication of silk fibroin and silk fibroin fluorescent nanofibers via
electrospinning

8. Bachelor of Science Program in Computer Science

COM01 Face, age and gender identification system for application 88
89
COM02 Text sentiment analysis from GoEmotions

Abstract Table of Contents Page

COM03 Improving digestive organ classification from wireless capsule 90
endoscopy images using deep learning
COM04 Development of a flood forecasting system in the lower Chao Phraya 91
River area
COM05 Autonomous simulated vehicles for steering angle prediction 92
COM06 Development of mobile application for daily air quality assessment 93
in Bangkok
COM07 2D environment mapping and self-position estimation with ultrasonic 94
range sensor array
COM08 Vacant parking slots detection using deep learning 95
COM09 Thai music mood analysis 96
COM10 READ2U: A text-to-speech mobile application for visually impaired 97
people
COM11 Trashy: A smart chatbot for sustainable trash management 98
COM12 Performance evaluation of face encoding techniques: a case study 99
COM13 Certificate verification using Ethereum blockchain system 100

9. Bachelor of Education Program in Mathematics 101
102
MATHED01 Creation of a regression equation for predicting a change in coastal 103
area in Samut Prakan
104
MATHED02 The relationships between the weight of the egg and the volume of the 105
albumen and the yolk 106

MATHED03 The mathematical model for determination of an appropriate number of
cars on each road lines at Srinakharinwirot University, Prasarnmit
campus

MATHED04 The relationship between the-15 puzzle and the permutation group

MATHED05 Geometric shapes obtained from swinging a simple pendulum

MATHED06 A mathematical model for COVID-19 pandemic controls

Table of Contents

Abstract Page

MATHED07 Study of parabolic chute propeller turbines 107
108
MATHED08 Investigating well-known football players’ foot placement angles for
taking free kicks

10. Bachelor of Education Program in Chemistry 109
110
CHEMED04 Learning about COVID-19 with “Defense against COVID-19” board 111
game

CHEMED07 Mangiferin structure improvement to increase the efficiency of water
solubility for reducing blood sugar levels

CHEMED08 Development of paper-based colorimetric device for detection of
proline content in honey

11. Bachelor of Education Program in Biology

BIOED01 Effect of herbal plant extracts and plant growth regulators on the 112
growth and development of Caladium bicolor Vent. 113
114
BIOED02 Development of a simple PCR-RFLP technique to determine genetic 115
relationship of some plants in Lamiaceae 116
117
BIOED03 Transcribing lab-modified DENV2 genome-mimicking construct via 118
in vitro transcription 119

BIOED04 Designing and synthesizing molecular constructs to mimic dengue
viral genome

BIOED05 DNA fingerprints of myotoxin-containing mushrooms in the genus
BIOED06 Russula
BIOED07
BIOED08 Anatomical and histological studies of the sand worm Perinereis
nuntia (Phyllodocida: Nereididae)

Comparative anatomy of leaf blade, petiole and wood of some species
of Bignoniaceae in Thailand

Comparative anatomy of some medicinal plants of the family
Sapindaceae in Thailand

Table of Contents

Abstract Page
120
BIOED09 Comparison of protease reaction efficiency from fruits and vegetables 121
BIOED10 affecting meat tenderness 122
BIOED11 123
BIOED12 Molecular identification and cluster analysis of edible boletes in Ubon 124
BIOED13 Ratchathani Province 125
BIOED14 126
BIOED15 Anatomical studies on selected Annonaceae medicinal plants in Thailand 127
BIOED16 128
BIOED17 Development of a simple PCR-RFLP marker technique to determine the 129
BIOED18 genetic relationship of some species in Cucurbitaceae 130
BIOED19 131
BIOED20 Anatomy of leaf blade and stem of the genus Trapa, Ammannia and
Rotala in Thailand

Molecular identification of myotoxin-containing mushrooms

Diversity of MSDIN family members in a hepatotoxic mushroom
(Amanita brunneitoxicaria)

Comparative relation between reproductive success and rarity of
orchids in Khao Soi Dao Wildlife Sanctuary

Comparison of leaf and wood anatomical characteristics of some
species of the tribe Uvarieae in Thailand

Anatomical investigation of vegetative parts of the tribe Miliuseae
(Annonaceae) in Thailand

Comparative in vitro seed germination and seedling development of
orchids in Khao Soi Dao Wildlife Sanctuary

Verification of somaclonal variation by RAPD marker and SCoT
marker of Dendrobium draconis Rchb.f. from tissue culture

12. Bachelor of Education Program in Physics

PHYED01 Wireless-power transfer experimental kit for undergraduate Physics 132
laboratory 133
134
PHYED02 The study of simple harmonic motion and damped oscillation by
using Phyphox application

PHYED04 Misconceptions in Torricelli's law applying in teaching-learning
activities in fluid mechanics

Table of Contents

Abstract Page
135
PHYED05 Viscosity measurement by underwater variable length pendulum
technique 136
PHYED06
An observation of the terminal velocity of a falling sphere in liquid by 137
PHYED07 using a mass scale
138
PHYED08 Learn how to determine the natural frequency of coupled pendulum 139
PHYED09 using a magnetic field sensor via a mobile application 140
PHYED10 141
PHYED11 Application of force sensors for stroke patient risk detection
PHYED12 142
PHYED13 Online experimental suite by the Internet of Things: IoT
143
Harmonograph simulation with Python

Investigating reflection and refraction of light by Arduino Wi-Fi
controller

Energy band gap of titanium dioxide nanotubes coating with gold
nanoparticles

Wetting properties of titanium dioxide nanotubes coating with gold
nanoparticles

13. Bachelor of Education Program in General Science

GSCED01 Development of online problem-based learning lessons to practice 144

environmental education process for lower secondary students topic 145
“The paper cycle from waste to benefits” 146
147
GSCED02 Development of multimedia to enhance learning on “using scientific 148
GSCED03 equipment” for 7th grade students 149

Development of the multimedia to enhance science learning on “How
to learn science” for 7th grade students

GSCED04 Development online lessons to practice scientific communication skills
topic “Stingless bee” for lower secondary students

GSCED05 Development of science lab experiments for lower secondary school
students topic transport substances in and out a cell

GSCED06 Development of online science lab experiment for lower secondary
school student topic Photosynthesis

Table of Contents

Abstract Page
150
GSCED07 The study of learning achievement of 7th grade students by using web- 151
GSCED08 based lessons on the topic of human beings and climate change
152
GSCED09 Learning management based on the STEAM education using the 153
GSCED10 seesaw application on electrical circuits to enhance product creation
and communication for upper primary students 154
GSCED11 155
GSCED12 Development of online science activity packages for lower secondary
school students topic junior doctor fight COVID-19

The development of grade 6 students’ problem-solving skills and
learning achievement in the topic of natural disasters using blended
learning

The development of online lesson on Weather Phenomena by using of
a Google Classroom for 7th grade students

Problem-based learning with questioning techniques to enhance learning
on matter in daily life of 5th grade students

SCIFAIR 2021
Transposition and inversion on Fibonacci numbers

Suwapat Inthrakumheang, Norramon Sawatchookawe, Narissara Benjasin and
Ruangvarin Intarawong Sararnrakskul *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
In this project, we studied the relationship between the musical note and the
Fibonacci numbers of how they were related through the transposition ( ) and
inversion ( ) functions on ℤ21. Moreover, we constructed the table of and
of {1, 1, 2, 3, 5, 8, 13, 21} on ℤ21 for investigation of hidden Fibonacci
numbers in some music.
Keywords: Fibonacci numbers, Inversion, Musical note, Transposition

1

SCIFAIR 2021
On the sums of reciprocal Lucas numbers

Kanokphon Khonsue, Methiya Traganpinitpong, Lapatsarada Iambenjasub and
Jittinart Rattanamoong *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
In this work we consider infinite sums derived from the reciprocals of the Lucas
numbers and infinite sums derived from the reciprocals of the square of the Lucas
numbers. Applying the floor function to the reciprocals of these sums, we obtain
equalities that involve the Lucas numbers.
Keywords: Floor function, Infinite sum, Lucas numbers, Reciprocal

2

SCIFAIR 2021
Dividing the perimeter of a triangle into unequal proportions

Nawinda Amadtohed, Phonthakorn Racha-in, Thitiphon Chaidee and Thunwa Theerakarn *

Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract

We classify the envelope of all line segments that divide the perimeter of a
triangle into the ratio α : (1 - α) as α varies from 0 to 1. If α is larger than the ratio

2

of the longest side length to the perimeter, then the envelope is a 12-sided closed

curve consisting of six line segments and six parabolic arcs. For other values of
α, the envelope is the union of one to three parabolic arcs and possibly a

5 or 9-sided non-closed curve consisting of line segments and parabolic arcs. This

work extends Berele and Catoiu's result in the case α = 1 where the envelope is
2

a 6-sided closed curve consisting of three line segments and three parabolic arcs.

Keywords: Bisecting-deltoid, Envelope, Partition, Perimeter, Triangle

3

SCIFAIR 2021
On solutions to the Diophantine equations + ( + 1) = 4
and + ( + ) = 2

Kanittanat Saowapaklimkul, Jinnawat Thaewthintong, Pattharapa Petcharat and
Sermsri Thaithae *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
In this project, we study the solution in positive integers of the Diophantine
equation + ( + 1) = 4 for primes ≥ 2 where , and are positive
integers persuading the condition that + = 2, 3, 4 and the solution in non-
negative integers of the Diophantine equation + ( + ) = 2 for primes
≥ 2 when = 2 and = 3 which , and are non-negative integers.
Keywords: Diophantine equations

4

SCIFAIR 2021
On nilpotent matrices satisfying a binomial-like matrix equation

Sadsawad Intrasod, Arif Doloh and Thitarie Rungratgasame *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
It is known that if two matrices satisfy the binomial-like matrix equation

then they have the same characteristic polynomial. This result leads to an open
problem to find a characterization for matrices satisfying the above equation.
In our work, we study the binomial-like matrix equation over nilpotent matrices.
We show that the result holds if 2 − 1 ≤ . However, it is not always true
for the cases where 1 ≤ ≤ 2 − 2. We then give some characterization only
for some cases. We also apply the Jordan canonical form of nilpotent matrices
to give some conditions where the binomial-like matrix equation holds.
Keywords: Binomial-like matrix equation, Jordan canonical form, Nilpotent
matrix.

5

SCIFAIR 2021
Measuring political gerrymandering by district average wasted
votes

Kannada Anan, Thararat Kaewkhamsai, Hathairat Khanthachan and Witsarut Pho-on *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
In this project, we introduce a new method to measure political gerrymandering
called district average efficiency gap ( ̅ ̅̅ ̅ ̅ ) for a two-party election with
districts. The ̅ ̅̅ ̅ ̅ is the average of (λ) (1 ≤ ≤ ) where (λ) is the
proportion of the difference between each party’s wasted votes to the total votes
in district .
Keywords: Efficiency gap, Political gerrymandering, Wasted votes

6

SCIFAIR 2021
The gambling dice game made fair

Padchareeya Ruangrujee, Ratchanon Phothipong, Sasithon Suksroem and
Nahathai Rerkruthairat *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
The objective of this research is to study about the average payoff of each player
in gambling dice game in case of fair and bias games. The player's payoff depends
on the money amount in the pot before rolling the dice. In this work, we will use
the product of the return that will occur in each play and the probability of rolling
the dice on each side to analyze the expected return of each player and determine
the value that will make this dice gambling game fair.
Keywords: Average payoff, Dice game

7

SCIFAIR 2021
Magic cubes and linear algebra

Praphon Kanokladarom, Siriyakorn Duangmoon, Natsima Srithaisong and
Suchada Pongprasert *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
A magic cube of order 4 is an arrangement of {1, 2, . . . , 64} in a 4 × 4 × 4 cube
such that the sums of the numbers in 16 rows, 16 columns, 16 pillars and 4
triagonals are the same. Using finite-dimensional vector spaces over the field 2,
we introduce the notion of affine magic cubes of order 4 and study their
properties.
Keywords: Affine magic cubes, Linear Algebra, Magic cube, Vector spaces

8

SCIFAIR 2021
Face colorings of triangular bipyramids and cubes with colors

Panjapon Boonsai, Theeraya Boonserm and Nissara Sirasuntorn *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Colorings of the edges of a tetrahedron with 3 colors have been counted. This
motivates us to determine colorings of faces of triangular bipyramids, cubes and
their variations. In this project, we study the rotational symmetries of these shapes
and count the number of distinct face colorings of them with colors.
Keywords: Colorings, Rotation symmetric

9

SCIFAIR 2021
The edge-sum game

Kanokwan sinsai, Waroot Tekitchamroon and Varanoot Khammanee *
Department of Mathematics, Faculty of Science, Srinakharinwirot University Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
The edge-sum labeling of a graph G with n vertices consists of labeling the
vertices of G with distinct integers from 1 to n such that, when each edge is
assigned as induced label the sum of the labels of its vertices, all induced edge
labels are distinct. The edge-sum game was introduced by Tuza in 2017 as a two-
players game on a nontrivial connected graph in which Alice and Bob take turns
labeling the vertices with distinct integers from 1 to n. Alice’s goal is to edge-
sum label the graph as Bob’s goal is to prevent it from happening. In this project,
we study winning strategies for Alice and Bob in fans, complete bipartite graphs,
cycles and paths.
Keyword: Edge label, Edge-Sum label, Vertex label

10

SCIFAIR 2021
Prediction of the number of Covid-19 confirmed cases in Thailand
by Box-Jenkins and Holt's exponential smoothing methods

Kunlarat Sapsermsong, Peticha Chavanangkool, Chananchida Wongpirapat and
Atchariya Watthanawisut *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
This paper compares the performance of ARIMA and Holt's exponential
smoothing models in terms of forecasting the number of Covid-19 cases in
Thailand. The data used is from the period of 1 January 2021 until 31 December
2021 reported by the World Health Organization (WHO) in Thailand. The results
show the ARIMA (0,1,1) model by the Box-Jenkins method has the lowest
MAPE values. However, Holt’s exponential smoothing model is an appropriate
fit for the data based on the lowest RMSE and MAE values. The forecast period
in this paper is from 1 January 2022 until 30 January 2022.
Keywords: Box-Jenkins, Covid-19, Holt’s exponential smoothing

11

SCIFAIR 2021
Factors affecting fertility rate of Thailand

Sirikorn Aeurfeur, Sitanan Darasuwannasri, Aphatsara Khangkhet and Karnchana Panichakarn *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
The objective of this research was to study the factors affecting the fertility rate
of Thailand. The data used in the research were secondary data of 77 provinces
in 2020, collected from the National Statistical Office. There were 21 studied
variables, consisting of one dependent variable, the fertility rate of Thailand and
20 independent variables. Multiple regression analysis was used to analyze the
data. The results showed that factors significantly influenced the fertility rate of
Thailand were male population rate, average expenditure per household, marriage
registration rate, female population graduating from pre-elementary school,
female population graduating from high school, farmer rate and southern region.
These factors can approximately explain 75.1% of the variability of the fertility
rate of Thailand. The multiple linear regression model can be written as follow:
̂ = −25.2 + 0.0834 − 0.932 + 0.104

−0.04492 − + 0.0426 −
0.023340

+1.103 ℎ
Keywords: Fertility rate of Thailand, Multiple regression analysis

12

SCIFAIR 2021
Factors influencing the drug offense cases rate in Thailand in the
year 2020

Pakjira Butkaew, Laksika Rattanamethawong, Sasicha Jirapiyasuwan and
Atchariya Watthanawisut *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
The purpose of this research was to study the factors influencing the drug offense
cases rate in 77 provinces of Thailand in the year 2020. The data used in the
analysis were secondary data from National Statistical Office. Multiple
regression analysis was applied to construct the predictive equation on factors
influencing the drug offense cases rate.
The result of the research shows that factors influencing the drug offense cases in
Thailand in the year 2020 are divorce registration rate (X1), alcohol addiction rate
(X2), bipolar disorder rate (X3), and the dummy of the region: north (D1), south
(D2), east (D3), west (D4), northeast (D5), and central (D6). These factors can
explain 45.57% of the variability of drug offense cases in Thailand. The multiple
regression equation is:

Ŷ = - 79.069 + 0.885Xdivore registration + 4.968Xalcohol dependence pateint rate
- 12.527Xbipolar diorder pateint rate + 336.204Dnorth + 245.208Dsouth
+ 265.044Deast + 141.356Dwest + 155.320Dnortheast + 128.968Dcentral

Keywords: Drug abuse cases, Multiple linear regression analysis

13

SCIFAIR 2021
Depression structural equation model with empirical data

Cheevanan Dangjanthuk, Preeyaporn Tarapoom, Phichita Srijan and Sukanya Intarapak *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
The purposes of this study were to examine the consistency of the developed
depression structural equation model with empirical data and to study the direct
effect, indirect effect, and the collective effect of social support, self-esteem,
stress, and resilience. The researchers collected data from 167 undergraduate
students of the Faculty of Science at Srinakharinwirot University. The statistical
analysis results of the structural equation model and the examination of the model
consistency showed that the developed depression structural equation model
conformed to the empirical data and self-esteem and resilience provided the
negative effects to depression directly while social support and stress provided
the positive effects to depression in direct and indirect ways but the stress had the
negative effects on depression indirectly. Moreover, self-esteem, resilience,
social support, and stress have interaction effects on depression.
Keywords: Depression, Resilience, Self-esteem, Social support, Stress

14

SCIFAIR 2021
Factors affecting satisfaction of students toward the Faculty of
Science, Srinakharinwirot University

Jutarat Muenpakdee, Teeranan Jawana, Metinee Nuengmatcha and Karnchana Panichakarn *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
This study aimed to investigate the factors affecting satisfaction of students
toward the Faculty of Science, Srinakharinwirot University. The sample group of
83 science students were required to complete an online questionnaire via google
form. The statistical analysis used to analyze the data were percentage, mean and
multiple regression analysis. The finding revealed that instructors, landscape and
environment, being fourth-year student, and building no.19 influenced
satisfaction of students toward the Faculty of Science. These factors affected the
score of satisfaction by 49.90%. The multiple linear regression model is

̂ = 15.668 + 1.322Xinstructors + 1.612Xlandscape and environment
+ 3.742Dfourth-year + 7.890Dbuilding19

Keywords: Faculty of Science, Multiple regression analysis, Srinakharinwirot
University, Satisfaction

15

SCIFAIR 2021
Forecasting model for the number of deceased from road accidents
in Thailand

Pasupol Pulros, Pitchayakorn Tongthumchat, Kanokwan Kritsadakornkul and
Sukanya Intarapak *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
This study aimed to create the suitable predictive model for forecasting the
number of deceased from road accidents in Thailand, by using monthly data total
of 126 months from January 2011 to June 2021. The statistical methods were
used; the Box-Jenkins method and the Winters exponential smoothing method,
both additive and multiplicative. The suitable forecasting model was considered
from the smallest value of Mean Square Error (MSE) and Mean Absolute
Percentage Error (MAPE). The results of the study show that Box-Jenkins
method with the model ARIMA(0,1,1)(2,1,0)12 excluding constants was the most
appropriate predictive model for forecasting the number of deceased from road
accidents in Thailand.
Keywords: Box-Jenkins, Road accidents, Winters exponential smoothing

16

SCIFAIR 2021
A study on factors affecting online football gambling behaviors of
undergraduate students Srinakharinwirot University

Wacharawit Phomul, Sakuna Chimmanee, Nirarat Muadkhum, Anan Thongnuam and
Atchariya Wattanavisut *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
This work was a study about the factors affecting online football gambling
behaviors of undergraduate students at Srinakharinwirot University. The
objectives of the study were: 1) to study the student’s online football gambling
behaviors, 2) to study the factors affecting student’s online football gambling
behaviors considering 5 factors: personal factors, self-control factors, social
bonding factors, social learning factors, and factors in online football gambling
channels, and 3) to create a regression equation showing the relationship of
variables in factors affecting students’ online football gambling behavior. This
study used a quantitative approach which collecting data from 183 undergraduate
students at Srinakharinwirot University by a questionnaire as a collecting tool.
Multiple regression analysis was used for data analysis. The study found that self-
control, social bonding, and social learning factors affect online football
gambling behavior among students. All three factors have a negative effect. It is
suggested that we should promote social bonding, self-control, and social
learning factors about online football gambling has declined to prevent and solve
online football gambling problems of undergraduate students at Srinakharinwirot
University. The researchers recommend that family institutions and educational
institutions are the main institutions for preventing and solving the problem of
online football gambling among undergraduates at Srinakharinwirot University.

Keywords: Football online, Gambling, Multiple regression analysis

17

SCIFAIR 2021
Forecasting model of the closing price of Bangkok Life Assurance
Public Company Limited

Nanapas Kongperm, Naphatsorn Suadet, Natnicha Tangsuntornkhan and Sukanya Intarapak *
Department of Mathematics, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
This research aimed to study the optimal forecasting for the closing price of
Bangkok Life Assurance Public Company Limited. The daily data used was from
2 January 2019 to 30 December 2021 reported by the Stock Exchange of
Thailand. Box-Jenkins and Holt’s exponential smoothing methods were
considered. In this research, the model was optimal based on the lowest values of
the mean absolute error and the absolute percentage error. It showed that the most
suitable forecasting model for the closing price of Bangkok Life Assurance Public
Company Limited was by Holt’s exponential smoothing method.
Keywords: Box-Jenkins, Closing price, Holt’s exponential smoothing

18

SCIFAIR 2021
Development of a high-performance liquid chromatography method
for determination of mangiferin

Orawan Naiyasit and Piyada Jittangprasert *
Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Mangiferin (C19H18O11) is a xanthone glucoside that mainly present in various
parts of mango (Mangifera indica L). It has numerous biological activities such
as antioxidant activity, antiviral activity, antidiabetic activity, antimicrobial
activity and anti-inflammatory. In this study, a simple high-performance liquid
chromatography (HPLC) method has been developed for the analysis of
mangiferin in crude mango leaves extract. The optimized separation system was
achieved on C18 column using 0.5 % acetic acid and methanol (75:25, v/v) as a
mobile phase. The wavelength at 316 nm was chosen as a detection wavelength.
The results have shown that mangiferin was separated completely at 5.0 min. The
linear calibration curve was obtained with high correlation coefficient over the
concentration range of 0.025-100 mg/L. The limit of detection (LOD) was 10.0
mg/L. The proposed method showed high intra-day and inter-day precisions with
satisfactory recovery results. The developed method was successfully applied for
determination of mangiferin in mango leaves extract.
Keywords: High-performance liquid chromatography, Mangiferin, Mango
leaves extract

19

SCIFAIR 2021
The synthesis Carbon Dots from mangosteen peel as a fluorescent
probe for irons determination

Kojchakorn Panthaphol, Kooljira Koekon and Itthipol Sungwienwong *
Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Carbon Dots (CDs) are novel nanomaterial that has gained interest due to their
unique properties including multi-color fluorescence, high photostability, high
solubility, low toxicity, and low cost. In this work, we proposed the synthesis of
CDs by hydrothermal method from mangosteen peel which is an agricultural
waste. The optimal conditions for the synthesis of CDs such as temperature, time,
and pH were investigated. The results showed that CDs synthesized at 200 oC, 30
min provided the highest fluorescent intensity. The CDs were also characterized
by spectroscopic methods including UV-visible spectroscopy, fluorescence
spectroscopy, and Fourier-transform infrared spectroscopy (FTIR). These CDs
demonstrated high photostability up to 4 weeks without significantly changed in
fluorescence intensity. In addition, the CDs can be utilized as a fluorescent probe
for selective determination of irons (Fe2+, Fe3+) in a linear range of 10 – 250 ppm.
Keywords: Agricultural waste, Carbon dots (CDs), Fluorescent probe, Mangosteen
peel

20

SCIFAIR 2021
Development of colorimetric method for the measurement of cysteine

Nanthiya Srichandee, Nareerat Thongsuk and Weena Siangproh *
Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Cysteine is an amino acid that contains the thiol group, which plays an important
role in the functioning of many important cellular functions and has been
implicated in many diseases. Therefore, the determination of cysteine in
biological samples is important. This work describes a novel method to determine
cysteine by colorimetry using a potassium ferricyanide ([Fe(CN)6]3-) and
methylene blue (MB) as a new reagent. The study indicates that at pH 7.0 of
phosphate-citrate buffer, Fe(III) is reduced to Fe(II) by cysteine and in situ
formed Fe(II) reacts with MB to give a dark blue soluble. A color change from
green to dark blue can be observed by the naked eye. The quantified concentration
was calculated using color intensity by the ImageJ program. Under the optimal
conditions, the linearity ranges from 0 to 50 ppm. This finding assay shows the
possibility of being an alternative method for the determination of cysteine
instead of using a high-cost instrument.
Keywords: Colorimetry, Cysteine, Methylene Blue, Phosphate-citrate buffer,
Potassium ferricyanide

21

SCIFAIR 2021
Design of novel coumarin derivatives as angiotensin converting
enzyme inhibitor

Kingkan Matwangsaeng, Piyaporn Arunkirirote and Pornthip Boonsri *
Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Angiotensin converting enzyme (ACE) is well known enzyme for its dual actions
in converting inactive angiotensin I to active angiotensin II and further degrade
active bradykinin (BK), which play a vital role in controlling the blood pressure.
Hence, ACE inhibitors become one of the most commonly used drugs for
hypertension such as Lisinopril, but side effects can occur when used for a long
time. Coumarin, a natural substance found in many plants, has been shown to
possess as anticoagulant, anticancer, antibacterial and anti-ACE activities. In the
present work, we are interested in designing a new coumarin derivatives as anti-
ACE agent called coumarin B. Then, binding energies and interactions between
coumarin derivatives and ACE were investigated by using molecular docking.
The obtained results exhibited that all the designed compounds showed good
binding affinities (-8.26 to -11.01 kcal/mol), which better than the reference drug
lisinopril. Interestingly, all docked compounds posed similar mode of binding to
the reference drug, which interacted to the key residues located at S1 pocket via
Ala354 Glu384 Tyr523 residues and S2’ pocket via Gln281 Lys511 His353
His513 Tyr520 and Zn701 residues. This finding was confirmed that our designed
coumarin derivatives can be further developed as the potent ACE inhibitors.
Moreover, in silico study indicated that all compounds pass the drug-like
properties. Finally, the ADME profile indicated that most of the coumarin
derivatives could be proposed as the lead molecule for hypertension.

Keywords: ADME, Angiotensin converting enzyme, Coumarin, Hypertension,
Molecular docking

22

SCIFAIR 2021
Effects of zinc sulfate on the production of lutein and canthaxanthin
in Chlorella zofingiensis

Ponlapat Chueathong, Nootchanath Wongsricha, Ratchadawan Thamrongcholpisit,
Sanyita Horata and Suthamat Niyompanich *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Lutein and canthaxanthin are antioxidant carotenoid pigments produced by
photosynthetic organisms. Under stress condition, the microalga Chlorella
zofingiensis can be able to generate high levels of carotenoids. However, no
previous studies have been reported on the effects of chemicals on lutein and
canthaxanthin production in C. zofingiensis. The aim of this study was to
determine the effects of zinc sulfate (ZnSO4) on microalgal biomass and the
production of lutein and canthaxanthin synthesis in C. zofingiensis cultivated in
mixotrophic conditions. Zinc sulfate was supplied at various doses of 2.5, 5, and
10 mM for 8 days. Microalgal cell growth was measured by a spectrophotometer
and calculated for the biomass concentrations. Subsequently, lyophilized cells
were extracted for pigment analysis of lutein and canthaxanthin using high-
performance liquid chromatography (HPLC). The results indicated that the
highest biomass concentration of 3.60 g/L was obtained at a concentration of 5
mM ZnSO4. When microalgae were grown at a concentration of 10 mM ZnSO4,
the lowest biomass concentration was found (2.73 g/L). All concentrations of
ZnSO4 2.5-10 mM had no effect on stimulating canthaxanthin production in C.
zofingiensis. Nevertheless, all those concentrations of ZnSO4 resulted in greater
lutein production with the highest lutein content of 2.76 mg/g DW at a dose of
2.5 mM ZnSO4. Under mixotrophic conditions, ZnSO4 could increase lutein
production in C. zofingiensis. Furthermore, this cultivation strategy could be used
to enhance the production of high-value compounds from microalgae.

Keywords: Canthaxanthin, Chlorella zofingiensis, Lutein, Zinc sulfate

23

SCIFAIR 2021
Leaf blade, petiole and wood anatomy of some species in the genus
Annona and Goniothalamus (tribe Annoneae (Annonaceae)) in
Thailand

Aratchaporn Prabpracha, Teerapat Panpeng and Anitthan Srinual *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
The anatomical characteristics of foliar, petiole and wood of some species of the
genus Annona and Goniothalamus (tribe Annoneae (Annonaceae)) were investigated.
The epidermis was conducted by peeling and clearing methods, then stained with
safranin. The transverse section of leaf blades and petioles were prepared by
paraffin method and stained with safranin and fast green. The wood specimens
were cut using a sliding microtome and stained with safranin. The results
indicated that the significant characteristics of leaf surface, leaf blade and petiole
in transverse sections for species identification were comprised of the
ornamentations of cuticle, the shapes of epidermal cells and cell walls, and the
presence or absence of trichomes and inclusions in epidermis, the distinctness or
indistinctness of cuticular layers on leaf margins, the curved leaf margin levels,
the presence or absence of secretory cells, closed or open rings of vascular bundle,
the shapes of the midribs and petioles, the shapes of vascular bundles in midribs
and petioles, the presence or absence of trichomes in midribs and petioles, and
the types of inclusions in midribs and petioles. Furthermore, the valuable wood
characteristics for identification were consisted of the grouping type of vessel,
the outline of solitary vessel, the arrangement intervessel pits, the presence or
absence of red stained inclusions in vessel, the type and strand length of axial
parenchyma, the type of inclusions in ray parenchyma cell, width of ray
parenchyma cell, the presence or absence of septate in fiber and thickness of fiber
walls.

Keywords: Anatomical characteristics, Annona, Goniothalamus, Plant identification

24

SCIFAIR 2021
Effect of drought stress on photosynthesis efficiency and the quantity
of bioactive compounds of Mentha cordifolia Opiz ex Fresen

Kamonchanok Chankhot, Phrukphan Niumnim and Sukhumaporn Saeng-ngam *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
The purpose of this study was to investigate the effect of drought stress on
photosynthesis efficiency and the quantity of bioactive compounds of Mentha
cordifolia Opiz ex Fresen. Completely randomized design (CRD) with four
replications was carried out at green house, Faculty of Science, Srinakharinwirot
University. There were two parts of this experiment. The first, drought stress
tolerance levels of plant at 0%, 3%, 6%, 12% and 15%(w/v) polyethylene glycol
6000 (PEG6000) in nutrient solution were used for evaluation of chlorophyll
fluorescence (Fv/Fm). The results showed that Fv/Fm in plants were decreased
when the increasing the concentration of PEG. Plants tolerated at the high level
of drought stress at 3%(w/v) PEG. Therefore, at the concentration of 3%(w/v)
PEG was used for the determination of the photosynthesis efficiency and the
quantity of bioactive compounds in the second part. The results found that Fv/Fm,
performance index (Pi), absorption flux per reaction center (ABS/RC) in drought
stressed plants were decreased at 15 days after drought stress and lower than the
control plants. Moreover, the high content of bioactive compounds in control
plants were D-germacrene, tetradecane, hexadecane and p-cymen-8-ol at 13.76%,
4.91%, 4.18% and 2.97%, respectively. The content of (E)-cubebene/D-
germacene/ β-copaene, D-limonene, caryophyllene and hexadecane at 10.60%,
7.73%, 3.62% and 2.18%, respectively in drought stressed plants. Therefore, this
study revealed that M. cordifolia tolerated drought stress at the 3%(w/v) PEG6000
and drought stress enhanced the quantitative and qualitative improvement of
bioactive compounds.

Keywords: Chlorophyll fluorescence, Drought stress, Essential oil

25

SCIFAIR 2021
PCR-based detection of hepatotoxic mushrooms in the genus
Amanita

Amonrat Lachompoo 1, Artittaya Boonnak 1, Sittiporn Parnmen 2 and Achariya Rangsiruji 1*
1 Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health,

Nonthaburi 11000
* Project Advisor Email: [email protected]

Abstract
The genus Amanita belongs to the family Amanitaceae (Basidiomycota, Agaricales)
which is distributed worldwide. Within this genus, both edible and deadly
poisonous species are found. Amanita brunneitoxicaria and A. exitialis are
hepatotoxic and are responsible for most cases of fatal mushroom poisoning in
Thailand. The lethal constituents of these poisonous mushrooms are cyclic
peptides encoded by the MSDIN family. Therefore, this study aimed to
differentiate A. brunneitoxicaria and A. exitialis from edible species based on the
presence of the MSDIN family. Eleven samples of Amanita were obtained and
identified as A. brunneitoxicaria, A. exitialis, and five edible species (A.
kotohiraensis, A. xanthogala, A. virgineoides, A. princeps, and A. vaginata) using
sequences of 28S rRNA gene and internal transcribed spacer region. Detection of
the MSDIN family revealed the presence of 300-bp PCR products in A.
brunneitoxicaria and A. exitialis, which were useful for discrimination of the
deadly poisonous amanitas.
Keywords: Amanita, Hepatotoxic mushroom, MSDIN family, PCR-based detection

26

SCIFAIR 2021
Effect of noise exposure on cognitive performance in college students

Piyada Hom-on, Metawee Jampawan, Irada Kingthong and Montree Maneepark *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Noise pollution contributes to unhealthy learning environments and has negative
impacts on students’ health and academic performance. These impacts of noise
on university students taking online classes during the COVID-19 pandemic have
often been neglected. This study aimed to investigate the effects of noise
disturbance on cognitive performance. Participants included 12 fourth-year college
students in the B.Sc. Biology Program. The study trial was a randomized, crossover
design. The cognitive performance, including the word list recall task and the
Stroop Color and Word Test (SCWT), was assessed using a remote platform. The
control and intervention subjects performed tasks during exposure to non-
distracting brown noise and polluted noise, respectively. There were no significant
differences between groups in the number of words recalled (mean [SD], 16.5
[3.9] words vs 17.4 [4.4] words; intervention vs control; P=.20) and in the
interference index (mean [SD], 8.2 [3.4] vs 7.6 [3.6]; intervention vs control;
P=.51). This study found that brief disturbing noise did not impair the cognitive
ability of college students. Further study is needed for evaluating the long-term
effects of noise pollution.
Keywords: Cognitive performance, Noise, Stroop Color, Word list recall task
and Word Test

27

SCIFAIR 2021
Identification of metabolic toxin-containing mushrooms based on
molecular techniques

Kantapong Authaiwan 1, Pimpakarn Laongdee 1, Supichaya Jiravanichkul 1,
Sittiporn Parnmen 2 and Achariya Rangsiruji 1*
1 Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health,

Nonthaburi, 11000
* Project Advisor Email: [email protected]

Abstract
Cantharocybe is a small genus of mushrooms containing only three known
species, C. gruberi, C. brunneovelutina and C. virosa. This genus has a worldwide
distribution in temperate and tropical regions. Cantharocybe virosa contains
coprine which can be induced by alcohol, resulting in metabolic toxicity
mushroom poisoning. Misidentification of this species for edible species in the
same genus as well as in another genus of Russula can occur due to their
morphological resemblance. Therefore, this study aimed to distinguish C. virosa
using molecular techniques. Eight samples of mushrooms were obtained from
the Ministry of Public Health. After DNA extraction, two loci of the internal
transcribed spacer (ITS) regions and nuclear-encoded large subunit ribosomal
DNA (LSU) were amplified and sequenced. Phylogenetic analysis based on a
combined dataset of ITS and LSU sequences confirmed that four samples were
the toxic species of C. virosa, while the other four samples were the edible
species of R. densifolia.
Keywords: Cantharocybe, ITS, LSU, Metabolic toxin-containing mushroom,
Phylogenetics

28

SCIFAIR 2021
Isolation of biosurfactant-producing bacteria from oil wastewater
in Bangkok

Thanarat Chookiattiyot and Somkiat Phornphisutthimas *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110
* Project Advisor Email: [email protected]

Abstract
Nowadays, the environmental crisis is important in the world and profoundly
impacts on human life. As a result, there are restrictions in the field of
agriculture, food, cosmetics and pharmaceutical industries. Industrial
manufacturers find the least cost of production methods, but they provide higher
amounts of chemical residues and have to use more budget to eliminate those
residues. The objective of this research was to reduce the use of chemical
surfactants in various industrial processes by selecting biosurfactant-producing
bacteria from oil wastewater sources in the area of Bangkok including
restaurants, grease traps, food spoilage. In this experiment. The wastewater
samples were diluted to a concentration of 10-3 to 10-5 and bacteria were cultured
with pour-plate technique in a selective medium, Mineral Salt Medium with
Methylene blue (MSM+MB) supplemented with 0.5% olive oil. The sample
plates were incubated at 37oC for 48 hours, and isolated colonies were restreaked
on the MSM+MB agar medium, following incubation at 37oC for 24-48 hours.
The results from 3 sources in Bangkok showed that there were 72 isolates on
the MSM+MB agar medium. It was a primary screening of biosurfactant-
producing bacteria including lipase activity to eliminate lipids and antifoam
activity to use in the industry and environment.

Keywords: Biosurfactant, Bacteria, Oil wastewater

29

SCIFAIR 2021
Characterization and expression analysis of Interferon regulatory
factor (IRF) gene from Macrobrachium rosenbergii

Saranchana Uamkaew and Parin Chaivisuthangkura *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Interferon regulatory factor (IRF) is a transcription factor that plays a pivotal role
in many aspects of the immune response, including immune cell development and
differentiation as well as regulating responses to pathogens. In this study, the IRF
gene from Macrobrachium rosenbergii (MrIRF) was functionally characterized
and identified. The sequence analysis indicated that MrIRF showed highest
identity to IRF from Penaeus vannamei (79.66%). The tissue expression and
distribution analysis using real-time RT-PCR exhibited that MrIRF was expressed
in all examined tissues (gill, hepatopancreas, heart, hemocyte, muscle, stomach,
and intestine) with the highest level in gill tissues and the lowest level in heart
tissues. The bacterial challenge experiment will be further investigated to
determine the role of MrIRF in the innate immune response of M. rosenbergii.

Keywords: Innate immune response, Interferon regulator factor (IRF),

Macrobrachium rosenbergii

30

SCIFAIR 2021
Characterization and expression analysis of Toll interacting protein
(Tollip) from Macrobrachium rosenbergii

Phakjeera Khanngern and Parin Chaivisuthangkura *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
Toll interacting protein (Tollip) is a crucial negative regulator of TLR-mediated
innate immune responses found in many organisms. Although several Tollip
genes have been identified in the crustaceans, none of them has been reported in
freshwater prawn, Macrobrachium rosenbergii. In this study, the Tollip gene
from Macrobrachium rosenbergii (MrTollip) was characterized and its biological
function against bacteria was investigated. The sequence analysis showed that the
partial MrTollip sequence shared 70.31% similarity to Penaeus monodon Tollip.
Real-time RT-PCR revealed a broad expression of MrTollip in all tested tissues,
including gill, heart, hepatopancreas, hemocyte, muscle, stomach, and intestine
with a significantly high level in gill and hepatopancreas. After bacterial
challenge with Aeromonas hydrophila, the relative expression of MrTollip
mRNA in gill was up-regulated from 12-24 hour post-injection (hpi) and reached
the highest level (3.8-fold) at 24 hpi. These results suggested that Tollip may play
an important role in the immune response of M. rosenbergii, especially in
antibacterial activity.
Keywords: Innate immunity, Macrobrachium rosenbergii, Toll interacting protein
(Tollip)

31

SCIFAIR 2021
Production of polyclonal antibodies against recombinant S8
structural protein of Tilapia lake virus (TiLV)

Kittapad Thiamsan and Siwaporn Longyant *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
The culture of Nile tilapia (Oreochromis niloticus) is an important industry in
many countries around the world, including Thailand. There is an outbreak of
tilapia lake virus (TiLV) in sixteen counties around the world causing massive
loss and mortality in tilapia. TiLV is a single-stranded RNA virus with 10
genomic segments. It infected and replicated in the target organs, gill, intestine,
and brain of the infected fish. The objective of this study was to produce the
polyclonal antibodies (PAb) for detecting the TiLV-infected fish. The
recombinant proteins of TiLV-segment 8 (S8) were expressed in E. coli and
purified for immunization in mice to produce the polyclonal antibody. The results
showed that the polyclonal antibodies against TiLV can bind with TiLV-S8 and
TiLV infected-fish proteins of 20 and 18 kDa, respectively. Therefore, this
synthesis polyclonal antibodies can be used to detect TiLV-infected tilapia using
Western blotting, dot blot, and immunohistochemistry.
Keyword: Immunization, Polyclonal antibody, Tilapia lake virus

32

SCIFAIR 2021
Generation of polyclonal antibodies against Salmonella enterica
serovar Virchow and S. enterica serovar Hadar

Patcharaporn Sangsuwan, Thunchanok Nuchphongsai and Siwaporn Longyant *
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110
* Project Advisor Email: [email protected]

Abstract
This research aims to produce polyclonal antibodies against two serovars of
Salmonella for detecting Salmonella Virchow and Salmonella Hadar. The outer
membrane proteins (OMP) of S. Virchow and S. Hadar were extracted and used
as antigens for mice immunization. Then the immune response to OMP of S.
Virchow and S. Hadar was determined by Western blotting. The results showed
that the mouse polyclonal antibodies can bind to S. Virchow and S. Hadar.
Therefore, the polyclonal antibodies generated in this study are expected to be
used as an immunological tool for detecting S. Virchow and S. Hadar pathogens
which are causative agents of salmonellosis to prevent and monitor the spread of
infection.
Keywords: Polyclonal antibodies, Salmonella Hadar, Salmonella Virchow

33