The 16th CU-TU MT Student's Research Conference

Contents 2
3
Message from Dean of the Faculty of Allied Health Sciences, Chulalongkorn University 4
Message from Dean of the Faculty of Allied Health Sciences, Thammasat University
Message from Dean of the Faculty of Allied Health Sciences, Burapha University 5
Message from Dean of the Faculty of Science and Technology,
Bansomdejchaopraya Rajabhat University 6
Message from Dean of the Faculty of Science and Technology, 7
Huachiew Chalermprakiet University 8
Conference Schedule 29
Abstract Contents 59
79
Clinical Microbiology and Parasitology (MB) 101
Clinical Immunology and Transfusion Medicine (lM) 118
Clinical Chemistry and Toxicology (CC) 136
Clinical Hematology and Microscopy (HM)
Miscellaneous (MS)
Organizing Committee

Page 1

Message from Dean of the Allied Health Sciences,
Chulalongkorn University

Dear Dean of Faculty of Allied Health Sciences, Thammasat University, Dean of Faculty of Allied
Health Sciences, Burapha University, Dean of Faculty of Science and Technology,
Bansomdejchaopraya Rajabhat university, Distinguished guests, Staff members, and medical
technology students

On behalf of the Faculty of Allied Health Sciences, Chulalongkorn University, I am delighted to
welcome everyone to the 4th CU–TU-BUU–BSRU Medical Technology Student’s Research
Conference (The 16th CU-TU MT Student’s Research Conference). This has become a
meaningful traditional conference which gather all medical technology students from 4
universities. The aim of this conference is to facilitate students to have a perspective on research
and innovation for diagnostic area. We are into the next new normal after we face with the
pandemic of COVID-19. Role of MT professional in healthcare system are undoubtedly. I hope
you will gain knowledge and learn new innovation and technologies which will help you all to
initiate our research project together.

I would like to take this opportunity to express my sincere thanks to invited speakers, students
and organizing committees.

Finally, I wish you have a successful the 4th Chulalongkorn- Thammasat- Burapha
Bansomdejchaopraya Rajabhat university University Medical Technology Student Research
Conference and empower networking in the near future.

Thank you very much
Palanee Ammaranond
Associate Professor Palanee Ammaranond, Ph.D.
Dean of Faculty of Allied Health Sciences, Chulalongkorn University

Page 2

Message from Dean of the Faculty of Allied Health Sciences,
Thammasat University

Dear Deans of Faculty of Allied Health Sciences, Chulalongkorn
University, Burapha University and Bansomdejchaopraya Rajabhat University, distinguished
guests, faculty members and students from all the universities. On behalf of the Faculty of
Allied Health Sciences, Thammasat University, I would like to congratulate Chulalongkorn
University for organizing the 4th Chulalongkorn-Thammasat-Burapha-Bansomdejchaopraya
Rajabhat University Medical Technology Student Research Conference 2021.

The conference had been first originated as “Chulalongkorn-Thammasat University
Medical Technology Student Research Conference” with the collaboration between
Chulalongkorn University and Thammasat University in 2005. The conference had been
focused on creating cultural academic interactions among medical technology students and
academic staff of the two institutes. Since 2018, the conference has been expanding with
our closed collaborations, Burapha University (2018), and Bansomdejchaopraya Rajabhat
University (2019), in which making the 16th Joint Conference participated by participants from
the four universities.

This annual event features many opportunities for learning and networking with
colleagues from several institutes in scientific and friendly atmosphere. It will also allow
students to learn from leading experts with backgrounds that vary from corporate executives
to individual practitioners on some of the most relevant topics related to the professions.
Furthermore, this joint conference also provides a precious opportunity for students to share
their research experiences, to build pride in their professions, to be confident to further
develop their professions in the future.

On behalf of the Faculty of Allied Health Sciences, Thammasat University, I would
like to take this opportunity to express my heartfelt thanks to all invited speakers, students
and the organizing committees from all the four universities. Thank you to everyone who is
lending their time and expertise to ensure a very meaningful and productive TU-CU-BU-BSRU
conference. The next conference will build on the success of this conference and my very
best wishes.

Warm Regards,
Assoc. Prof. Plaiwan Suttanon, PhD.
Dean of the Faculty of Allied Health Sciences, Thammasat University

Page 3

Message from the Dean
The Faculty of Allied Health Sciences, Burapha University

Dear Dean of Faculty of Allied Health Sciences, Chulalongkorn University,
Thammasat University, and Dean of Faculty of Science and Technology,
Bansomdejchaopraya Rajabhat University, all faculty members, and
students from the four Universities

This is the fourth year of the academic conference “4th Chulalongkorn-Thammasat-Burapha-
Bansomdejchaopraya University Medical Technology Student Research Conference.” Although this 2021
year will be the second year disrupted by the COVID-19 global pandemic. Among these pandemic,
medical technology and laboratory professionals play a vital role. We have still encouraged to continually
this conference in virtual meeting that is not only sharing our research experiences and knowledge on
medical technology but also strongly collaborating between teachers and students.

On behalf of Dean of Faculty of Allied Health Sciences, Burapha University, I am proud to be part
of a university to have the valuable opportunities to work with all participants from various academics.
Clearly, this virtual conference and academic affairs will inspire the creativity to our students and
professional advising to our students in Medical Technology. Finally, I wish you success this academic
ambiance and the sustainable development of knowledge in the field of medical technology in the
present and future.

Regards,

Asst. Prof. Marut Tangwattanachuleeporn, Dr.rer.nat
Dean of Faculty of Allied Health Sciences, Burapha University

Page 4

Message from the Dean of Faculty of Science and Technology,
Bansomdejchaopraya Rajabhat University.

Dear Dean of Faculty of Allied Health Sciences, Chulalongkorn University, Thammasat University,
Burapha University and all students.

It is my great pleasure and honour to join this meeting again. We are very happy to be the
member of this traditional conference as our students have a very good opportunity to learn
and gain experiences. Last year, we have to participate online as the COVID-19 situation
problem. The problem remain till this year so we have to meet online again. Anyway ,I hope
that our students still can learn and gain experiences from this conference.
I would like to express my sincere thanks to the committee who organized the conference this
year even there are many problems on the way as the COVID-19 situation still spread every
where in our country.
Next year, we will be the host to organize this conference. I hope that the COVID-19 situation
will be settled soon so that next year we can welcome all of you to meet onsite at BSRU
campus. Then, we will try our best to arrange and manage the conference as good as the
previous hosts have done it. So, I’m looking forward to seeing all of you at BSRU campus.

Yours sincerely,

Associate Professor Dr.Boonmee Kavinseksan
Dean of Faculty of Science and Technology,Bansomdejchaopraya Rajabhat University.

Page 5

Message from the Dean
The Faculty of Medical Technology, Huachiew Chalermprakiet University

Dear Dean of Faculty of Allied Health Sciences, Chulalongkorn University,
Thammasat University, and Dean of Faculty of Science and Technology,
Bansomdej Chaopraya Rajabhat University, all faculty members, and students from the
four Universities

On behalf of the Faculty of Medical Technology, Huachiew Chalermprakiet University and
our students, we appreciate the opportunity to participate in the 4th annual CU, TU, BUU, BSRU
Medical Technology Student Research Conference. It was our honor and privilege to present and
share our research results with students and faculty from Chulalongkorn, Thammasat, Burapha,
and Bansomdet Chaopraya Rajabhat Universities. This event served as an excellent forum for our
undergraduate students to disseminate knowledge acquired during their research while enhancing
their presentation skills and interacting with their peers. It's my hope that we can use this and
future interuniversity events, to improve the quality and standards of research as well as enhance
the undergraduate experience.

Finally, The Faculty and Students of Medical Technology, Huachiew Chalermprakiet
University, would like to Thank you. We are most grateful to have attended this event. We sincerely,
hope that the Medical technology students in attendance, will be inspired by this experience and
continue to study and apply new knowledge as they move forward on their journey of self-
development.

Regards,

Asst. Prof. Suwanna Semsri, Ph.D.
Dean of Faculty of Medical Technology, Huachiew Chalermprakiet University

Page 6

Conference Agenda
The 4th CU–TU-BUU–BSRU Medical Technology Student’s Research Conference
(The 16th CU-TU MT Student’s Research Conference)

December 23, 2021 (8 AM – 5 PM)

Online Platform by ZOOM

...............................................

08.00 – 08.30 Registration

08.30 - 08.40 Report by Program Chair of Medical Technology,

Faculty of Allied Health Sciences, Chulalongkorn University

08.40 – 08.55 Welcoming and Opening Remarks by Dean of Faculty of Allied Health

Sciences, Chulalongkorn University

08.55 – 09.00 Photo Group Shot via ZOOM
09.00 – 10.00 Special Lecture “Disease X and Medical Technologists’ roles”

(Supaporn Wacharapluesadee, MT, Ph.D)

10.00 – 10.10 Morning break

10.10 – 11.30 Oral Presentations by Student Delegates (BRSU, BUU, TU and CU)

11.30 – 12.30 Lunch break

12.30 – 14.10 Research Presentations by Medical Technology Students (Session 1)

14.10 – 14.20 Afternoon break

14.20 – 16.00 Research Presentations by Medical Technology Students (Session 2)

16.00 – 16.20 Closing Ceremony and Certificate Presentation Ceremony

16.20 – 16.30 Closing Remarks by Dean of Faculty of Science and Technology,

Bansomdejchaopraya Rajabhat University

16.30 – 17.00 Recreational Activities by the Medical Technology Students

Page 7

Page 8

Poster Poster titles Uni Page
no. CU 30

Clinical microbiology and parasitology CU 31
CU 32
MB1 Direct detection of Escherichia coli producing extended CU 33
spectrum beta lactamase in pig feces by Recombinase CU 34

polymerase amplification (RPA) BUU 35
BUU 36
MB2 Rapid and naked eye nucleic acid detection for bacterial
contamination in platelet products BUU 37
BUU 38
MB3 Study of Antimicrobial peptides in Reptiles
BUU 39
MB4 Effect of Roasted and Green Arabica Coffee Bean Extract in
antimicrobial activity CU 40

MB5 In vitro Antifungal Susceptibility Profile: Is there any difference in
MIC values among mycelial form and yeast form of Sporothrix
schenckii?

MB6 The efficiency of essential oils from herb against spoilage
bacteria in fresh shrimp.

MB7 Novel development of differential medium to accelerate the
isolation of Lodderomyces elongisporus and Candida
parapsilosis yeast, which are difficult to isolate using
conventional media

MB8 Prevalence of Lodderomyces elongisporus in pigeon dropping
from Chonburi province, Thailand

MB9 The effect of bitter bean (Parkia speciosa Hassk) peel extracted
to inhibit Pseudomonas aeruginosa , Serratia marcescens ,
Bacillus cereus and Plesiomonas shigelloides

MB10 The comparison of the sensitivity of recombinase polymerase
amplification and conventional polymerase chain reaction for
rotavirus detection

MB11 Detection of efflux pump genes in clinical isolates of
Acinetobacter baumannii

Page 9

Poster Poster titles Uni Page
no.

MB12 Direct detection of Escherichia coli producing Extended CU 41
Spectrum beta- lactamase in pigs’ feces by polymerase chain

reaction

MB13 Development of droplet digital PCR for detection of CU 42
Plasmodium falciparum histidine-rich protein 2 deletion

MB14 Making perfect fungal slide by a novel and simple modified agar CU 43
block smear technique

MB15 Screening of fluconazole susceptibility of Candida spp. from a CU 44

direct hemoculture using molecular detection methods

MB16 The characterization of Cyt2Aa toxin from Bacillus thuringiensis BUU 45

for the binding to various cell membrane

MB17 Anti-bacterial activity of local rice extracts of Sakaro province BUU 46

MB18 Antibacterial activity of Artemisia lactiflora extract against BUU 47
Staphylococcus aureus TU 48
TU 49
MB19 Bioinformatics analysis of the Morganella morganii in response
to cadmium toxicity based on proteome data TU 50
TU 51
MB20 Detection of Colistin gene and Carbapenemase gene in TU 52
Extended Spectrum Beta-Lactamase producing Escherichia coli
and Klebsiella pneumoniae TU 53

MB21 Comparison of COX1 gene sequences of Strongyloides
stercoralis in Thailand with Genbank database

MB22 Analysis of protein-protein interaction network of malaria
parasite during intraerythrocytic development cycle

MB23 Detection of SARS-CoV-2 and Bacteria on Highly Contaminated
Surfaces in Thammasat University Rangsit Campus and Nearby
Areas

MB24 Investigation of 12 novel compounds for antimicrobial effect on
Streptococcus suis

Page 10

Poster Poster titles Uni Page
no. TU 54
TU 55
MB25 Plasmodium knowlesi genetic diversity and susceptibility of
human infection. A systematic review CU 56
BSRU 57
MB26 Study of mechanism of Ca2+ mobilization between ER-
mitochondria induced by nonstructural 2B protein of enterovirus BSRU 58
A71
BUU 60
MB27 Development of droplet digital PCR for detection of BUU 61
Plasmodium falciparum Kelch mutations CU 62
CU 63
MB28 A survey of Toxoplasma gondii oocyst and other intestinal CU 64
65
parasites from cat’s feces in Bang Sai Kai community and nearby 66
areas 67

MB29 Molecular Identification of Carotenoid-producing yeasts;
Rhodotorula mucilaginosa

Clinical immunology and transfusion medicine

IM30 Effect of Phyllanthus amarus Supplements on Interferon-
Gamma Production by T Cell in Obese Subjects

IM31 Prevalence of latent tuberculosis infection (LTBI) of foreign

workers in Chonburi province

IM32 Oxidized High-density Lipoprotein Concentration in Type 2

Diabetes Mellitus

IM33 Antioxidant and anti-inflammatory activities of edible mushroom
Astraeus Asiaticus

IM34 Recombinase Polymerase Amplification of RhD subtype DEL

IM35 Detection of predicted Coa and Cob antigens using molecular TU
techniques TU
TU
IM36 Factors Affecting Continuous Donations among Thammasat
University Hospital Donors

IM37 An approach to blood utilization and blood preparation at
Pakkred Hospital: 3 years experience (2018- 2020)

Page 11

Poster Poster titles Uni Page
no. 68
69
IM38 The optimization of nanoparticle encapsulation for drug delivery TU 70
system 71
72
IM39 Development and evaluation of quality control material for TU 73
hematocrit testing with hematocrit centrifuge 74
75
IM40 Development of Paper - Based Immunoassay with Immunogold CU 76
77
Silver Enhancement for Oxidized LDL detection. 78

IM41 Antitumor activity of Pleurotus Sojor-Caju on triple negative CU 80
breast cancer cell line
81
IM42 The Immunomodulatory effect of Pleurotus sajor-caju crude CU
extract in human monocyte

IM43 Characterization and limitation study of simultaneous paper- CU
based by Rh blood typing device

IM44 Effect of Lysiphyllum strychnifolium (Craib) A. Schmitz extract on BSRU

reducing proinflammatory cytokine in THP-1 cell line

IM45 Study on T follicular helper cell response to SARS-CoV-2 TU
vaccination

IM46 Transcriptome profiling of macrophages response to TU
Cryptococcus neoformans infection

IM47 Delivery routes of tumor antigen in Dendritic cells TU

IM48 Proteomic analysis of plasma proteins in human TU
immunodeficiency virus-infected patients under antiretroviral
therapy CU
CU
Clinical chemistry and toxicology

CC49 Association between DPYD gene polymorphisms and clinical
toxicity in Thai colorectal cancer patients treated with 5-
Fluorouracil-based regimen

CC50 Development of pyocyanin sensors for point of care testing
(POCT)

Page 12

Poster Poster titles Uni Page
no. CU 82
CU 83
CC51 The underlying mechanism of bisphenol-A on dysregulation of CU 84
gene expression associated with autism spectrum disorder (ASD)
CU 85
CC52 Bioinformatics analysis of ferroptosis-related genes and their BUU 86
transcriptional expression levels in cholangiocarcinoma BUU 87
CU 88
CC53 Optimization of Liquid chromatography-tandem mass
CU 89
spectrometry (LC-MS/MS) for discrimination of human steroid
hormones isoforms analysis. BUU 90
BUU 91
CC54 Comparison of Antioxidant capacity in Kaempferia parviflora TU 92
TU 93
Extracts,using Ethanol, Acetone and Hexane TU 94

CC55 The mutagenic effect of the ethanol extract from Parkia

speciosa empty pods.

CC56 Effects of Phyllanthus amarus Capsule Supplements on Lipid
Profile and Blood Glucose in Obese Subjects

CC57 Production and characterization of cell-penetrating antimicrobial
peptide from yeast for treatment of S.aureus and MRSA
infection

CC58 The effectiveness of artificial intelligence (AI) analysis on
screening and subtyping of autism spectrum disorder (ASD)
patients for precision medicine

CC59 The mutagenic effect of the Paper longum ethanol extract

CC60 Product design of salbutamol test kit using chemical reaction for
salbutamol contamination in meat

CC61 Applications of dried blood spot for diabetes follow-up program
via telemedicine

CC62 Study of lead-induced oxidative stress in human renal epithelial
cells.

CC63 Effects of long-term ketogenic diets on cardiovascular risk in
type 2 DM: A systematic review

Page 13

Poster Poster titles Uni Page
no. TU 95
TU 96
CC64 The suitable non-invasive markers for advanced hepatic fibrosis HCU 97
in type 2 diabetic patients (systematic review) CU 98
CU 99
CC65 Study of control material matrices containing red blood cells for CU 100
finger-stick blood glucose meters
TU 102
CC66 Effects of Boiling and Storage on Antioxidant Activity and Total CU 103
CU 104
Phenolic Content of Ginger Juice BSRU 105
CU 106
CC67 Protective role of natural products against oxidative stress in CU 107
Caenorhabditis elegans Part 1 CU 108
CU 109
CC68 Protective role of natural products against oxidative stress in
Caenorhabditis elegans Part 2

CC69 Optimization of liquid-liquid extraction method for human
steroid hormones analysis using LC-MS/MS

Clinical hematology and microscopy

HM70 Hematological parameters in Sarcopenia elderly: Systematic
Review and Meta-Analysis

HM71 Updated prevalence of thalassemia in Thailand

HM72 Anti-leukemic activity of rutin on human acute lymphoblastic
cells.

HM73 The in vitro anticoagulant effect of red rice bran extract on
human blood

HM74 CU Parasite E-learning for practicing on anatomy of Nematode,
Trematode and Cestode

HM75 Hemolytic effects of herbs in G6PD-deficient erythrocytes

HM76 Effect of Pinocembrin on the secretion of Interleukin-2 (IL-2) in
T-cell lymphoblastic leukemia (T-ALL)

HM77 Effect of Pinocembrin on the secretion of pro-inflammatory and
apoptotic cytokine; Tumor necrosis factor (TNF-α) in Jurkat cells

Page 14

Poster Poster titles Uni Page
no. TU 110
TU 111
HM78 Identification of ETV6-RUNX1-like subtype in children with B-cell
precursor acute lymphoblastic leukemia TU 112
TU 113
HM79 Study on the correlation between SNPs on DNA mismatch repair CU 114
gene and Signaling pathway gene with colorectal cancer risk in CU 115
Thais in the Lower Northeastern Region BUU 116
HCU 117
HM80 Correlation of dysmorphic red blood cells and hematuria for the
prognosis of glomerulonephritis BUU 118
BUU 119
HM81 Assessment of coagulopathy for effectively evaluate severity of BUU 120
BUU 121
patients with COVID-19 infection: A systematic review BSRU 122

HM82 An in vitro study for hemostatic property of oxidized bacterial

cellulose

HM83 Anti-cancer activity of Quercetin on human leukemic cells

HM84 Oxidative stress and antioxidant responses in elderly with
anemia and thalassemia carriers

HM85 The Study and Development of White Blood Cell Differential
Application

Miscellaneous

MS86 Extraction collagen from tilapia fish (Oreochromis niloticus )
scale for tissue engineering

MS87 Role of Durio zibethinus (durian rind extract) for developing
bioactive compounds

MS88 Physical and chemical contamination and biological hazard in
fresh seafood from the local fish market in Chonburi province

MS89 Study of Saccharum sinense Roxb. extract against anti-oxidant
and anti-microbial activities.

MS90 Effect of Phyllanthus amarus Schumach. & Thonn. on Hydrogen
peroxide-induced oxidative stress in the human Keratinocyte

Page 15

Poster Poster titles Uni Page
no. BSRU 123
MS91 Effect of red sticky rice (Oryza sativa L.) bran extract (RRBE) on BSRU 124
MS92 cell viability of H2O2-treated Huh7 cells BSRU 125
MS93 The Detection of Antibiotics in Pacific white shrimp in the TU 126
MS94 Thonburi region, Bangkok TU 127
MS95 The Detection of Antimicrobial agent in Chicken meat in TU 128
MS96 Thonburi region, Bangkok
TU 129
MS97 The latent fingerprint analysis by VeriFinger 12.0 Software TU 130
MS98
Assessment of information system requirement of medical TU 131
MS99 laboratory equipment and material management TU 132
MS100 Mini Data-mining: Discriminating Iron overload, Hypochromic BSRU 133
MS101 microcytic anemia and Normal individual using RBC Indices in BSRU 134
MS102 Pra-Intaracha populations
Evaluating the effectiveness of Lac dye for developing Bloody
fingermark
The development of questionnaire on knowledge and attitudes
among Thai adolescents towards the ban on blood donation
from gay and bisexual men
Development of questionnaire of knowledge, attitude, and
willingness in blood donation among Thai gay and bisexual men
Age-associated DNA methylation in colorectal cancer: A
systematic review
The Detection of Antimicrobials agent in Pork in the Thonburi
region, Bangkok
The Study of the diversity of the genetic code and the chemical-
physical properties of peroxidase enzymes.

Page 16

Oral presentation

Uni. Poster Title Time

no.

1 BSRU MS92 The Detection of Antibiotics in Pacific white shrimp in the 10.10-

Thonburi region, Bangkok 10.30

2 BUU MB10 The comparison of the sensitivity of recombinase 10.30-
polymerase amplification and conventional polymerase 10.50

chain reaction for rotavirus detection

3 TU MB20 Detection of Colistin gene and Carbapenemase gene in 10.50-
Extended Spectrum Beta-Lactamase producing 11.10

Escherichia coli and Klebsiella pneumoniae

4 CU CC67 Protective role of natural products against oxidative 11.10-
stress in Caenorhabditis elegans 11.30

Page 17

Zoom Poster Title Time
ROOM Uni
CU no.

CU MB1 Direct detection of Escherichia coli producing 12.30-
CU
CU extended spectrum beta lactamase in pig feces by 12.50
CU Recombinase polymerase amplification (RPA)

Zoom 1 MB2 Rapid and naked eye nucleic acid detection for 12.50-
BUU
BUU bacterial contamination in platelet products 13.10

BUU MB3 Study of Antimicrobial peptides in Reptiles 13.10-
BUU 13.30

MB4 Effect of Roasted and Green Arabica Coffee Bean 13.30-
Extract in antimicrobial activity 13.50

MB5 In vitro Antifungal Susceptibility Profile: Is there any 13.50-
difference in MIC values among mycelial form and 14.10
yeast form of Sporothrix schenckii?

Coffee break

MB6 The efficiency of essential oils from herb against 14.20-
spoilage bacteria in fresh shrimp. 14.40

MB7 Novel development of differential medium to 14.40-
accelerate the isolation of Lodderomyces 15.00
elongisporus and Candida parapsilosis yeast, which

are difficult to isolate using conventional media

MB8 Prevalence of Lodderomyces elongisporus in 15.00-

pigeon dropping from Chonburi province, Thailand 15.20

MB9 The effect of bitter bean (Parkia speciosa Hassk) 15.20-
peel extracted to inhibit Pseudomonas aeruginosa, 15.40

Serratia marcescens, Bacillus cereus and
Plesiomonas shigelloides

Page 18

ROOM Uni Poster Title Time
CU no.
CU
MB11 Detection of efflux pump genes in clinical isolates 12.30-
CU of Acinetobacter baumannii 12.50

CU MB12 Direct detection of Escherichia coli producing 12.50-
CU Extended Spectrum beta- lactamase in pigs’ feces 13.10
Zoom 2 by polymerase chain reaction
BUU
MB13 Development of droplet digital PCR for detection 13.10-
BUU of Plasmodium falciparum histidine-rich protein 2 13.30
BUU deletion
TU
MB14 Making perfect fungal slide by a novel and simple 13.30-
modified agar block smear technique 13.50

MB15 Screening of fluconazole susceptibility of Candida 13.50-
spp. from a direct hemoculture using molecular 14.10
detection methods

Coffee break

MB16 The characterization of Cyt2Aa toxin from Bacillus 14.20-
thuringiensis for the binding to various cell 14.40

membrane

MB17 Anti-bacterial activity of local rice extracts of Sakaro 14.40-
province 15.00

MB18 Antibacterial activity of Artemisia lactiflora extract 15.00-
against Staphylococcus aureus 15.20

MB19 Bioinformatics analysis of the Morganella morganii 15.20-
in response to cadmium toxicity based on 15.40
proteome data

Page 19

ROOM Uni Poster Title Time
TU no.

TU MB21 Comparison of COX1 gene sequences of 12.30-
Strongyloides stercoralis in Thailand with Genbank 12.50
TU
database
TU
TU MB22 Analysis of protein-protein interaction network of 12.50-
Zoom 3 malaria parasite during intraerythrocytic 13.10
TU
development cycle

MB23 Detection of SARS-CoV-2 and Bacteria on Highly 13.10-
Contaminated Surfaces in Thammasat University 13.30

Rangsit Campus and Nearby Areas

MB24 Investigation of 12 novel compounds for 13.30-
antimicrobial effect on Streptococcus suis 13.50

MB25 Plasmodium knowlesi genetic diversity and 13.50-
susceptibility of human infection. A systematic 14.10

review

Coffee break

MB26 Study of mechanism of Ca2+ mobilization between 14.20-

ER-mitochondria induced by nonstructural 2B 14.40
protein of enterovirus A71

CU MB27 Development of droplet digital PCR for detection 14.40-

of Plasmodium falciparum Kelch mutations 15.00

BSRU MB28 A survey of Toxoplasma gondii oocyst and other 15.00-
intestinal parasites from cat’s feces in Bang Sai Kai 15.20

community and nearby areas

BSRU MB29 Molecular Identification of Carotenoid-producing 15.20-
yeasts; Rhodotorula mucilaginosa 15.40

Page 20

ROOM Uni Poster Title Time
BUU no.

BUU IM30 Effect of Phyllanthus amarus Supplements on 12.30-
CU Interferon-Gamma Production by T Cell in Obese 12.50
CU
CU Subjects

Zoom 4 TU IM31 Prevalence of latent tuberculosis infection (LTBI) of 12.50-
TU foreign workers in Chonburi province 13.10
TU
IM32 Oxidized High-density Lipoprotein Concentration in 13.10-
TU Type 2 Diabetes Mellitus 13.30
TU
IM33 Antioxidant and anti-inflammatory activities of 13.30-

edible mushroom Astraeus Asiaticus 13.50

IM34 Recombinase Polymerase Amplification of RhD 13.50-

subtype DEL 14.10

Coffee break

IM35 Detection of predicted Coa and Cob antigens using 14.20-

molecular techniques 14.40

IM36 Factors Affecting Continuous Donations among 14.40-
Thammasat University Hospital Donors 15.00

IM37 An approach to blood utilization and blood 15.00-
preparation at Pakkred Hospital: 3 years experience 15.20

(2018- 2020)

IM38 The optimization of nanoparticle encapsulation for 15.20-
drug delivery system 15.40

IM39 Development and evaluation of quality control 15.40-
material for hematocrit testing with hematocrit 16.00
centrifuge

Page 21

ROOM Uni Poster Title Time
CU no.
IM40 Development of Paper - Based Immunoassay with 12.30-
CU 12.50
CU Immunogold Silver Enhancement for Oxidized LDL
CU detection. 12.50-
IM41 Antitumor activity of Pleurotus Sojor-Caju on triple 13.10
TU negative breast cancer cell line 13.10-
Zoom 5 IM42 The Immunomodulatory effect of Pleurotus sajor- 13.30
caju crude extract in human monocyte 13.30-
BSRU IM43 Characterization and limitation study of 13.50
simultaneous paper-based by Rh blood typing
TU device 13.50-
TU MS99 Development of questionnaire of knowledge, 14.10
TU attitude, and willingness in blood donation among
TU Thai gay and bisexual men 14.20-
14.40
Coffee break
IM44 Effect of Lysiphyllum strychnifolium (Craib) A. 14.40-
15.00
Schmitz extract on reducing proinflammatory 15.00-
cytokine in THP-1 cell line 15.20
IM45 Study on T follicular helper cell response to SARS- 15.20-
CoV-2 vaccination 15.40
IM46 Transcriptome profiling of macrophages response 15.40-
to Cryptococcus neoformans infection 16.00
IM47 Delivery routes of tumor antigen in Dendritic cells

IM48 Proteomic analysis of plasma proteins in human
immunodeficiency virus-infected patients under
antiretroviral therapy

Page 22

ROOM Uni Poster Title Time
CU no.

CU CC49 Association between DPYD gene polymorphisms 12.30-
CU and clinical toxicity in Thai colorectal cancer 12.50

CU patients treated with 5-Fluorouracil-based regimen

Zoom 6 CC50 Development of pyocyanin sensors for point of 12.50-
CU care testing (POCT) 13.10

CU CC51 The underlying mechanism of bisphenol-A on 13.10-
dysregulation of gene expression associated with 13.30
BUU autism spectrum disorder (ASD)
BUU
CC52 Bioinformatics analysis of ferroptosis-related genes 13.30-
and their transcriptional expression levels in 13.50
cholangiocarcinoma

Coffee break

CC53 Optimization of Liquid chromatography-tandem 14.20-

mass spectrometry (LC-MS/MS) for discrimination of 14.40
human steroid hormones isoforms analysis.

CC54 Comparison of Antioxidant capacity in Kaempferia 14.40-

parviflora Extracts,using Ethanol, Acetone and 15.00
Hexane

CC55 The mutagenic effect of the ethanol extract from 15.00-

Parkia speciosa empty pods. 15.20

CC56 Effects of Phyllanthus amarus Capsule 15.20-

Supplements on Lipid Profile and Blood Glucose in 15.40
Obese Subjects

Page 23

ROOM Uni Poster Title Time
CU no.
CC57 Production and characterization of cell-penetrating 12.30-
BUU antimicrobial peptide from yeast for treatment of 12.50
BUU CC59 S.aureus and MRSA infection
CC60 The mutagenic effect of the Paper longum ethanol 12.50-
TU extract 13.10
MS100 Product design of salbutamol test kit using 13.10-
Zoom 7 TU chemical reaction for salbutamol contamination in 13.30
TU CC61 meat
TU CC62 Age-associated DNA methylation in colorectal 13.30-
CC63 cancer: A systematic review 13.50
TU
CC64 Coffee break 14.20-
TU Applications of dried blood spot for diabetes 14.40
CC65 follow-up program via telemedicine 14.40-
Study of lead-induced oxidative stress in human 15.00
renal epithelial cells. 15.00-
Effects of long-term ketogenic diets on 15.20
cardiovascular risk in type 2 DM: A systematic
review 15.20-
The suitable non-invasive markers for advanced 15.40
hepatic fibrosis in type 2 diabetic patients
(systematic review) 15.40-
Study of control material matrices containing red 16.00
blood cells for finger-stick blood glucose meters

Page 24

ROOM Uni Poster Title Time
HCU no.
CU
CC66 Effects of Boiling and Storage on Antioxidant 12.30-
CU Activity and Total Phenolic Content of Ginger Juice 12.50
CU
Zoom 8 CC58 The effectiveness of artificial intelligence (AI) 12.50-
TU analysis on screening and subtyping of autism 13.10
CU spectrum disorder (ASD) patients for precision
CU
BSRU medicine

CC68 Protective role of natural products against oxidative 13.10-
stress in Caenorhabditis elegans Part 2 13.30

CC69 Optimization of liquid-liquid extraction method for 13.30-
human steroid hormones analysis using LC-MS/MS 13.50

Coffee break

HM70 Hematological parameters in Sarcopenia elderly: 14.20-
14.40
Systematic Review and Meta-Analysis

HM71 Updated prevalence of thalassemia in Thailand 14.40-
15.00

HM72 Anti-leukemic activity of rutin on human acute 15.00-

lymphoblastic cells. 15.20

HM73 The in vitro anticoagulant effect of red rice bran 15.20-
extract on human blood 15.40

Page 25

ROOM Uni Poster Title Time
CU no.
CU
CU HM74 CU Parasite E-learning for practicing on anatomy of 12.30-
CU Nematode, Trematode and Cestode 12.50

Zoom 9 TU HM75 Hemolytic effects of herbs in G6PD-deficient 12.50-
TU erythrocytes 13.10

TU HM76 Effect of Pinocembrin on the secretion of 13.10-
TU
Interleukin-2 (IL-2) in T-cell lymphoblastic leukemia 13.30
(T-ALL)

HM77 Effect of Pinocembrin on the secretion of pro- 13.30-

inflammatory and apoptotic cytokine; Tumor 13.50
necrosis factor (TNF-α) in Jurkat cells

Coffee break

HM78 Identification of ETV6-RUNX1-like subtype in 14.20-
children with B-cell precursor acute lymphoblastic 14.40
leukemia

HM79 Study on the correlation between SNPs on DNA 14.40-
mismatch repair gene and Signaling pathway gene 15.00
with colorectal cancer risk in Thais in the Lower

Northeastern Region

HM80 Correlation of dysmorphic red blood cells and 15.00-

hematuria for the prognosis of glomerulonephritis 15.20

HM81 Assessment of coagulopathy for effectively 15.20-
evaluate severity of patients with COVID-19 15.40

infection: A systematic review

Page 26

ROOM Uni Poster Title Time
no.

CU HM82 An in vitro study for hemostatic property of 12.30-
oxidized bacterial cellulose 12.50

CU HM83 Anti-cancer activity of Quercetin on human 12.50-
leukemic cells 13.10

BUU HM84 Oxidative stress and antioxidant responses in 13.10-

elderly with anemia and thalassemia carriers 13.30

HCU HM85 The Study and Development of White Blood Cell 13.30-
Differential Application 13.50

Coffee break

Zoom BUU MS86 Extraction collagen from tilapia fish (Oreochromis 14.20-
10 BUU niloticus ) scale for tissue engineering 14.40

MS87 Role of Durio zibethinus (durian rind extract) for 14.40-
developing bioactive compounds 15.00

BUU MS88 Physical and chemical contamination and biological 15.00-
hazard in fresh seafood from the local fish market 15.20
in Chonburi province

BUU MS89 Study of Saccharum sinense Roxb. extract against 15.20-
anti-oxidant and anti-microbial activities. 15.40

BSRU MS102 The Study of the diversity of the genetic code and 15.40-
the chemical-physical properties of peroxidase 16.00
enzymes.

Page 27

ROOM Uni Poster Title Time
no.

BSRU MS90 Effect of Phyllanthus amarus Schumach. & Thonn. 12.30-
on Hydrogen peroxide-induced oxidative stress in 12.50

the human Keratinocyte

BSRU MS91 Effect of red sticky rice (Oryza sativa L.) bran 12.50-
extract (RRBE) on cell viability of H2O2-treated 13.10

Huh7 cells

BSRU MS93 The Detection of Antimicrobial agent in Chicken 13.10-
meat in Thonburi region, Bangkok 13.30

BSRU MS101 The Detection of Antimicrobials agent in Pork in the 13.30-
Thonburi region, Bangkok 13.50

Coffee break

Zoom TU MS94 The latent fingerprint analysis by VeriFinger 12.0 14.20-
11 TU Software 14.40
14.40-
MS95 Assessment of information system requirement of
medical laboratory equipment and material 15.00
management

TU MS96 Mini Data-mining: Discriminating Iron overload, 15.00-
Hypochromic microcytic anemia and Normal 15.20
individual using RBC Indices in Pra-Intaracha

populations

TU MS97 Evaluating the effectiveness of Lac dye for 15.20-

developing Bloody fingermark 15.40

TU MS98 The development of questionnaire on knowledge 15.40-
and attitudes among Thai adolescents towards the 16.00

ban on blood donation from gay and bisexual men

Page 28

Page 29

Direct detection of Escherichia coli producing extended spectrum beta lactamase in pig feces by
Recombinase polymerase amplification (RPA)

Kodchamon suthidee, Weeraya Panyameesamer, Nuntaree Chichanawongsaroj
Program of Medical Technology, Faculty of Allied Health Sciences, Chulalongkorn University

The dissemination of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is now an
important global health issue. ESBLs is an enzyme which is produced from antimicrobial resistance genes that can
be transmitted to humans through their food consumption. Food-producing animals, including pigs, are significant
reservoirs of antimicrobial resistance (AMR).

According to the reasons mentioned, the rapid detection of ESBLs is required for efficient epidemiological
control and treatment. In this study, multiplex recombinase polymerase amplification (RPA) combined with a single-
stranded tag hybridization chromatographic printed-array strip (STH-PAS), as a lateral flow strip assay (LFA), was
established for the rapid and simultaneous detection of multiple bla genes in a single reaction. Visible blue lines,
indicating the presence of the blaCTX-M, blaSHV and blaOXA genes, were observed within 10 minutes by the naked eye.

The multiplex recombinase polymerase amplification (RPA) was carried out using TwistDx basic kit,
specific primers for blaCTX-M, blaSHV, blaOXA and positive control DNA. The reaction was incubated at 37 ˚C for 25 min.
After that, an LFA strip was inserted into a developing solution containing RPA amplicons. The results revealed
three visible blue lines at each position marker. Thus, RPA-LFA could be further tested with the DNA samples
extracted from pigs’ feces and will finally validate the results with PCR-sequencing.
Keywords: Escherichia coli, Extended spectrum beta lactamase (ESBL), Recombinase polymerase amplification

(RPA), Lateral flow strip assay (LFA), blaCTX-M, blaSHV and blaOXA genes

MB

Page 30

Rapid and naked eye nucleic acid detection for bacterial contamination in platelet products

Pichayada Sawasdee, Supphawet Tangjirawattana, Panan Ratthawongjirakul
Program of Medical Technology, Faculty of Allied Health Sciences, Chulalongkorn University

Bacterial contamination in platelet products is currently the major cause of platelet transfusion infections,
which is life-threatening. A bacterial contamination screening of platelet products must be conducted for every unit
before being used to ensure platelet quality and safety. However, diverse bacterial contamination detection
methods are impractical in the blood banking working area and take a long turnaround time, not matching the
platelet's short shelf life. The objective of this study will assess the effectiveness of the Helicase-dependent
amplification combined with the SYBR green I (HDA/SYBR green I) for the detection of bacterial contamination in
platelet products. The examination will be based on 40 platelet products collected from the King Chulalongkorn
Memorial Hospital and the spiked platelet products. All the samples will be aliquoted for BacT/Alert system testing.
HDA/SYBR green I is expecting to demonstrate good sensitivity and specificity when detecting bacterial
contamination in platelet products compared to BacT/Alert system, with no cross-reaction to other microorganisms.
Keywords: Bacterial contamination, platelet products, Helicase-dependent amplification, SYBR green I

MB

Page 31

Study of Antimicrobial Peptides in Reptiles

Pojjananat Sukkhang , Pawarin Aeknam , Khaemaporn Boonbumrung
Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University

Antimicrobial-resistant bacteria (AMR) are a critical issue in medical treatment also affect the
environment, especially in the food chain. The misuse of antibiotics in farming and major in related food
industries was associated with an increase of AMR. The alternative agents are still discovering in parallel to
cope with urgent issues. Unfortunately, the discoveries are not keeping up with the facing situation. It was found
that the last line of antibiotics is reducing in susceptibility to antibiotics, according to AMR reports in many
countries around the world. Natural substances are used as a model for the development of antibiotics due to
their antimicrobial properties. Many types of research are focusing on antimicrobial peptides (AMPs) as the
smaller structure than the conventional agents. The therapeutic function of AMPs in various mechanisms are
receptor-binding peptides, membrane-active peptides, cell-wall inhibit peptides, and other inhibitory
mechanisms. Interestingly, one-third of the AMPs are derived from frogs. Most of the amphibians and reptiles
have been in a long evolution. The immune systems of these animals were interesting to discover in terms of
against infection. In this literature review, we have compiled a list of antimicrobial agents from the past and
present, divided by the Order of reptile which consists of Order Testudinata (Chelonia) – turtles, Order
Crocodilia - crocodiles and allies, Order Rhynchocephalia – tuatara and Order Squamata - snakes and lizards.
The information was presented in the major classes of antimicrobial peptides including defensins, cathelicidins,
liver-expressed peptides, and others. The antibacterial or antiviral activity was identified in some peptides and
provided in genetic information by others. This review is a spotlight on the new and powerful antimicrobial
peptides, with chanced to develop in an antibiotic in the future.

Keywords: Antimicrobial peptides(AMPs) , Reptiles , Antimicrobial-resistant bacteria(AMR) , Antibiotic

MB

Page 32

Effect of Roasted and Green Arabica Coffee Bean Extract in antimicrobial activity

Nutsaraporn Yaiyong, Nichagorn Leekhammong, Nuntanut Meungkrot, Siriporn Chuchawankul, Navaporn Worasilchai
Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University

Coffee arabica is among the most traded commodity, consumed beverages, and active ingredients in
many skin care products worldwide including Thai society. Besides its stimulating effect, antimicrobial and
anti-aging activities caused by its various bioactive compounds especially caffeine (major component of
coffee beans) has been studied for decades in roasted-coffee bean. In addition, green coffee bean became
popular for weight loss. It contains many substances known as chlorogenic acids and one of its byproducts
have strong antioxidant properties which provide medical and wellness benefits. However, there is little
information on the effect of the roasting process (a heating step that bring out the aroma and flavor locked
inside the seed) on its bioactivity, and its safety in cell-based model system. In this research, we aim to
examine the antimicrobial activity and assess its cytotoxicity in vitro in various coffee bean’s roast level: extra
dark roast, dark roast, medium roast, and light roast which were defined by the roasting peak temperature at
475oF, 445oF, 420oF, 400oF, and peaberry seeds which are the unroasted coffee beans. The coffee extracts
were brewed by using 1) an espresso machine, 2) moka pot, and tested against five common strains of
microbes: bacteria (Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922), fungi (Candida
albicans ATCC 90028, Aspergillus flavus ATCC 204304, and Fonsecaea pedrosoi ATCC 46428),
respectively. Modified broth microdilution and agar cultivation were used to test the extracts determining the
minimal inhibitory concentrations (MICs) and minimal microbicidal concentrations (MMCs). By in vitro, the
most antimicrobial activity has been observed in the extract of green coffee bean. It is noted that the higher
roasted temperature of coffee bean shows lower antimicrobial activity. This finding indicates the potential of
the coffee extracts as a novel candidate of disinfectant. However, the compound expressing those activities
need to be studied in the future.
Keywords: Arabica, Green coffee bean, Roasted coffee bean, Antimicrobial activity, Cytotoxicity

MB

Page 33

In vitro Antifungal Susceptibility Profile: Is there any difference in MIC values among mycelial
form and yeast form of Sporothrix schenckii ?

Chutchanok Sonkumharn1, Boossapasorn Boonyasirisri1, Ariya Chindamporn2, Navaporn Worasilchai1
1Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University
2Department of Microbiology, Faculty of Medicine, Chulalongkorn University

Sporothrix schenckii is a common causative agent of subcutaneous mycosis named sporotrichosis.
Based on its morphological characteristic, this microbe is classified as dimorphic fungus, filamentous form
and yeast form (infective form) are presented at environmental temperature (25oC-30oC) and in mammalian
host condition (35oC-37oC), respectively. To control the disease, antifungal drugs remain the recommended
treatment protocol based on in vitro susceptibility profile of filamentous form. So far even there is no standard
susceptibility protocol for dimorphic fungi, using mycelium phase is the common practice in microbiology
laboratory because of the simple obtain. Due to the different form of microbe between outside and inside the
host body, in this study we aim to compare the antifungal activity in vitro between filamentous form and yeast
form against 6 common antifungal drugs: Amphotericin B, Fluconazole, Itraconazole, Voriconazole,
Posaconazole, and Terbinafine. The SensititreTM YeastOne was used for minimal inhibitory concentration
(MIC) values. The agreement percentages of each drug were calculated among MIC values obtained from
filamentous form and yeast form. By the triplicate experiments, itraconazole is the most sensitive agent
against the tested S. schenckii (n=52) whereas fluconazole is the less sensitive one. In term of correlation,
very high agreement percentage of susceptibility profiles among 2 forms were presented. It showed as 88.4%
(Amphotericin B), 76.9% (Fluconazole), 90.1% (Itraconazole), 60.2% (Voriconazole), 77.4% (Posaconazole),
and 88.8% (Terbinafine). Only 2-fold dilution of MICs difference was observed which is acceptable in term of
hand-on practice. Moreover, it was likely that yeast form is more susceptible to antifungal drugs than
filamentous form. In conclusion, susceptible profile obtained from in vitro susceptibility test using mycelium
form can represent the susceptible profile of yeast form (infective form) which presents in mammalian hosts.
Even there is some difference in MIC values obtained among filamentous and yeast forms, no statistically
significant was demonstrated.

Keywords: Sporothrix schenckii, Susceptibility Test, Antifungal Agent, Filamentous form, Yeast form

MB

Page 34

The Efficiency of Essential Oils from Herb Against Spoilage Bacteria in Fresh Shrimp

Salinthip Riangsanthia, Thanathon Siricharoenwattanakorn, Kongkiat Aiamsaart, Palatip Chutoam
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

Currently, Thailand is exporting more seafood due to consumers' preference for seafood, especially
shrimp, which is the country's top exporter. Nevertheless, the spoilage of fresh shrimp by pathogenic bacteria
including Salmonella spp., Pseudomonas spp., Staphylococcus aureus, Bacillus spp., Vibrio spp. and Aeromonas
spp.is the main problem of the exported shrimp. So, to prolonging the life of fresh shrimp the preservation methods
are used such as preservative substances including Nitrate, Benzoate and Sulfite but these substances are often
harmful to health. From that reason, the nature substances are the choice to replacing the chemical substances,
especially essential oils from bergamot, lime and lemongrass, which found to have action against microorganisms
that cause food spoilage. Unfortunately, there no data on the effect of these essential oil to inhibit the pathogenic
bacteria which cause spoilage in fresh shrimp. So, this research aims to test the effect of essential oils from the
types of herbs, include bergamot, lime and lemongrass to inhibit the pathogenic bacteria which cause spoilage in
fresh shrimp. To test the inhibitory effect of essential oils, disc diffusion method, Minimal inhibitory concentration
MIC) and Minimal bactericidal concentration (MBC) were used to study in Salmonella spp., Pseudomonas
aeruginosa., Staphylococcus aureus, Bacillus cereus. By which, mm diameter disc which contains essential oils

microliter per disc were used to test the inhibitory activity of essential oil by dish diffusion method. The results
showed that essential oil from lemongrass showed the best inhibition of all bacteria tested, with the diameters of the
inhibition zone in the range of > mm, followed by the essential oils from lime and bergamot, respectively. Next,
lemongrass essential oils were selected to study the minimum inhibition concentration (MIC) and minimum
bactericidal concentration (MBC) against the tested organisms. The results showed that, the MIC values were equal
to the MBC values at , , and v/v), respectively. This indicates bactericidal activity of the essential oil
from lemongrass against the tested organisms. The data from this research show that lemongrass essential oil is a
natural substance, which has the potential to be developed as an antibacterial substance that causes spoilage of
chilled fresh shrimp.
KEYWORDS: Essential oil/ Chilled Shrimp/ Antibacterial activity

Page 35

Novel development of differential medium to accelerate the isolation of Lodderomyces elongisporus
and Candida parapsilosis yeast, which are difficult to isolate using conventional media

Aroonvadee Chaipasee, Kanokwan Prachongchat, Sahassawadee Sensomsri, Natthapaninee Thanomsridetchai
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

Lodderomyces elongisporus is an uncommon but potentially fatal cause of bloodstream infection in
immunocompromised hosts or in patients with intravenous access devices. In addition, L. elongisporus is distinct
but has phenotypically closely related to Candida parapsilosis making it difficult to identify using conventional
methods. Misidentification of L. elongisporus could have led to inappropriate patient management causing an
increased risk of mortality. To overcome this challenge, we developed a new, simple, and low-cost of differential
culture medium for the preliminary identification of L. elongisporus prior molecular confirmation. The novel
differential medium was developed to differentiate the L. elongisporus from C. parapsilosis based on the
biochemical properties of assimilate sugars and distinct colony morphological characteristics. Three species of
yeasts including L. elongisporus ATCC#11503, C. parapsilosis, and C. glabrata (as a control species) were
cultured on a new culture medium named as Sucrose-Maltose-Lactose-Bromocresol purple agar (or SMLB agar)
containing important components such as sucrose, maltose, and lactose. Upon fungal growth on SMLB agar, the
three species of yeasts showed markedly different characteristics. At 48h post-inoculation, L. elongisporus
ATCC#11503 appeared as green colonies, and culture medium changed from deep purple to partially yellow. C.
parapsilosis appeared as yellow colonies and culture medium changed from deep purple to yellow. C. glabrata
appeared as blue colonies and the color of culture medium did not change. Therefore, a low-cost SMLB agar for
detection and differentiation of L. elongisporus, C. parapsilosis, and C. glabrata has been developed. The utility
of the novel SMLB agar will be further evaluated for identification of L. elongisporus in clinical specimens as well
as in environment samples.
Keywords: Lodderomyces elongisporus, Candida parapsilosis, Candida glabrata, novel differential media

MB

Page 36

Prevalence of Lodderomyces elongisporus in pigeon droppings from Chonburi province,
Thailand

Amonrada Koonjom, Apinya Inrit, Thanyaporn Praditpong, Natthapaninee Thanomsridetchai
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

Lodderomyces elongisporus is an opportunistic fungus that can cause invasive as well as
bloodstream infections particularly in immunocompromised individuals. It formerly was common to misidentify
the L. elongisporus as Candida parapsilosis leading to inappropriate treatments and increased the risk of
disease severity. L. elongisporus can be isolated from free-living wild birds such as pigeons, which live
closely to humans, especially in public areas. Previous studies have highlighted that pigeon droppings in
public areas are great pathogen reservoirs. Therefore, it is an urgent need to determine the potential
environmental health risks of L. elongisporus distribution in pigeon droppings. This study aimed to investigate
the diversity of the fungal pathogens and identify the species of L. elongisporus in pigeon droppings from 11
districts of Chonburi province, Thailand from April to June, 2021. Pigeon dropping samples were collected
and isolated the fungal pathogens by culturing techniques on Sabouraud Dextrose Agar (SDA) plates. The
isolates were then characterized based on colony morphologies. Suspected colonies with a possible
identification of L. elongisporus were selected as white, embossed, smooth-edged yeast colonies with 1-2
mm diameter, which further identified the species as blue-turquoise colonies using CHROMagarTM. The
selected colonies were further identified using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight
(MALDI-TOF). Our results revealed that there was 41 out of 335 isolates (or 12.24%) from 178 pigeon
droppings samples identified as L. elongisporus. The positive isolates were collected from Amphoe Mueang
Chon Buri, Sriracha, Sattahip, Phanthong, Phanat Nikhom, Bo Thong, and Nong Yai, Chonburi province,
Thailand. Therefore, this study provides valuable information on L. elongisporus distribution in pigeon
droppings in Chonburi province, Thailand. Further study is required to understand the pathogenicity of these
isolates using suitable experimental models.
Keywords: Lodderomyces elongisporus, prevalence, pigeon droppings

MB

Page 37

The Effect of bitter bean (Parkia speciosa Hassk) peel extracted to inhibit Pseudomonas
aeruginosa, Serratia marcescens, Bacillus cereus and Plesiomonas shigelloides

Panumas Sakhmpee, Tatiya Udompaiboonlarp, Sopa Sala, Palatip Chutoam
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

Gastrointestinal infections are major mortality cause in children in every year. Nowadays there is
information indicating that bacteria in gastrointestinal disease are continuously increasing to drug resistance and
limited treatment options. The use of natural products is another approach to treatment, as natural products are
more chemically diverse than antibiotics. This makes the infection less likely to be resistant to drugs and cause

fewer side effects on the body. The data from previous study found that bitter bean (Parkia speciosa Hassk) peels
extract can inhibit the pathogenic bacteria in gastrointestinal disease. Therefore, in this study, we were interested
in studying the effect of bitter bean peel extract that inhibits pathogenic bacteria in gastrointestinal diseases, using
the disk diffusion method and determine the minimum inhibitory concentration (MIC) and the minimum bactericidal
concentration (MBC) values. The objective of this study was to study the bacterial inhibition of ethanol extracted
bitter bean peels extract against pathogenic bacteria in gastrointestinal disease including P. aeruginosa,
S. marcescens, B. cereus, and P. shigelloides, and to determine the MIC and MBC values of ethanol extracted
bitter bean peels extract against tested bacteria. The antimicrobial activity of the bitter bean peels extract was

determined by using agar disk diffusion, MIC and MBC techniques. By which, agar disk diffusion technique was
tested by a sterile paper disk containing 20 microliters of bitter bean peels extract per disk. Whereas, broth dilution
method and drop plate method was used to test MIC and MBC, respectively. The result found that, the extract from
the bitter bean peels inhibited all test bacteria The inhibition zone ranged from 13 to 27 millimeters on agar disk
diffusion method The MIC MBC of 95 ethanol extracted bitter bean peels extract against P. aeruginosa,
B. cereus, and P. shigelloides were 25 50 mg ml, 1 6 25 mg ml and 3 125 3 125 mg ml, respectively. Unfortunately,
the MIC and MBC values of S. marcescens could not be determined, which might come from the result that,
S. marcescens produce tannase to cause tannin biodegradation in bitter bean peels extract From the overall results

in this study indicated that the bitter bean peels extract has antibacterial activity to inhibit the growth of
gastrointestinal bacteria, that might be use as replacement material to control the Gastrointestinal infections in
Thailand.

Keywords: Bitter bean, Parkia speciosa Hassk peels extract, Antimicrobial activity against pathogenic bacteria in

the gastrointestinal disease MB

Page 38

The Comparison of the Sensitivity of Recombinase Polymerase Amplification and
conventional Polymerase Chain Reaction for Rotavirus Detection

Nutsuda Noimeesook , Piyapat Sa-ing , Wachiraporn Khaoluang , Uraiwan Intamaso
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

Food is essential to life; hence food products should be safe and uncontaminated with harmful
microbes or agents. To ensure food safety and prevent foodborne illness, the detection of pathogenic agents
should be rapid and accurate started from farm to forks. The objectives of this study were to evaluate the
sensitivity of the Recombinase Polymerase Amplification (RPA) and conventional Polymerase Chain Reaction
(PCR) assay for the detection of Rotavirus, a common foodborne pathogen associated with acute
gastroenteritis in humans. Template cDNA was cloned into pET-28b(+) and transformed to E. coli DH5 as
competent cells. Recombinant plasmids of Rotavirus-pET28b were extracted and confirmed by restriction
analysis, PCR, and sequencing. Each of the 10-fold serial dilutions of the purified Rotavirus-pET28b plasmid
was used to evaluate the sensitivity of RPA and PCR. Amplification products were visualized by agarose gel
electrophoresis. Results showed that RPA detected Rotavirus-pET28b plasmid diluted up to 10-1, equivalent to
1.458 x 1010 copies per reaction. In contrast, PCR amplification products could not be visualized by agarose
gel electrophoresis. RPA is not sensitive to unpurified genomic templates and can be performed in the
presence of PCR inhibitors. In addition, RPA can be conducted at a constant temperature without the need for
a thermocycler. Therefore, RPA is compatible with the fieldwork or low-resource setting.
Keywords: Food safety, Rotavirus, Cloning, Recombinase Polymerase Amplification (RPA), Polymerase Chain
Reaction (PCR)

MB

Page 39

Detection of efflux pump genes in clinical isolates of Acinetobacter baumannii

Saralchana Buhsaban , Salintip Athiwatthanawong , Rachaneeporn Tiyawisutsri
Program of Medical Technology, Faculty of Allied Health Sciences, Chulalongkorn University

Acinetobacter baumannii infection is a serious public health problem worldwide including Thailand. It
is one of the most important causes of nosocomial infection and has increasing resistant to many antimicrobial
agents. There are many antimicrobial resistances mechanism, efflux pump is a frequent mechanism for
antimicrobial resistance in A. baumannii. The aim of this study to investigated the prevalence of efflux pump
genes in A. baumannii isolates such as Resistance-nodulation-cell division (RND) efflux pumps system (adeB
and adeG), multidrug and toxic compound extrusion (MATE) family (abeM) and major facilitator superfamily
(MFS) efflux pump system (tetB) by polymerase chain reaction. RND efflux pumps system showed to be
associated with decreased susceptibility to a broad spectrum of antimicrobials such as tetracycline,
erythromycin, chloramphenicol, trimethoprim, fluoroquinolones, aminoglycosides and some -lactams. RND
efflux pumps system play an important role in multidrug resistant A. baumannii.
Keyword: Acinetobacter baumannii, Efflux pump, Multidrug resistance

MB

Page 40

Direct detection of Escherichia coli producing Extended Spectrum beta-
by polymerase chain reaction

Natcha Chayaburakul1, Siriyakorn Chiemchit1 , Nuntaree Chaichanawongsaroj 1
Program of Medical Technology(or department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University.

Escherichia coli producing extended spectrum beta-lactamase (ESBLs), a major mechanism of beta-
lactam resistance are an increasing problem worldwide which can be transmitted to human by the consumption
animals.Pigs are one of the most important reservoirs of antimicrobial resistance (AMR) because of misusing
antibiotics in farm. Detection of ESBLs genes are rapid diagnosis for treatment, surveillance and
epidemiological control. The objective of this study was to compare the incidence of blaCTX-M, blaTEM, blaSHV and
blaOXA using multiplex PCR between antibiotic treated and non-antibiotic treated pig farms. The PCR
amplification was carried out at PCR condition. The PCR reaction composed of 0.2 µM of forward and reverse
primer, 200 µM dNTPs, 0.625 U Taq polymerase and DNA template in total volume of 25 µl. The positive control
DNA of blaCTX-M, blaOXA, blaSHV and blaTEM showed the amplicon sizes of 593 bp, 814 bp, 868 bp and ,931 bp,

ected
from antibiotic treated and non-antibiotic treated farms.

Keywords: Extended Spectrum beta- lactamase (ESBL),Pigs,Polymerase chain reaction(PCR) ,
Escherichia coli , blaCTX-M, blaTEM,blaSHV, blaOXA

MB

Page 41

Development of droplet digital PCR for detection of Plasmodium falciparum histidine-rich
protein 2 deletion

Nuttakarn Yaowalak1, Natchanon Khanmasom1, Suttipat Srisutham1
1Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University
2Faculty of Tropical Medicine, Mahidol University

Malaria is still one of the important infectious diseases in Thailand because of Plasmodium spp.
infections (including P. falciparum, P. vivax, P. ovale, P. malariae and P. knowlesi). Rapid diagnostic tests
(RDTs) are the most widely used to identify P. falciparum based on immunochromatographic recognition of P.
falciparum histidine-rich protein 2 protein. However, deletion of Pfhrp2 gene may result in false-negative RDT
leading to misdiagnosis and delayed treatment. Thus, the prevalence of Pfhrp2 gene deletion would be useful
for the malaria diagnostic program. The droplet digital PCR (ddPCR) assay is a novel technique based on the
water-oil emulsion. A reaction is fractionated into 20,000 droplets and PCR amplification of the template
molecules occurs in each individual droplet which can be used for the detection and quantification of DNA
target. The aim of this study is to develop the droplet digital PCR (ddPCR) assay for the detection and
quantification of Pfhrp2 gene. The ddPCR reaction was prepared in total 20 L per reaction containing the
ddPCR reagents (10 L of Bio-Rad 1X ddPCR Supper Mix, 0.9 M of forward and reverse primers, and 0.25
M of probe) and 2 L of the DNA sample. To optimize the PCR annealing temperature, a temperature gradient
PCR of 64°C, 63°C, 62°C, 60°C 58°C, 56°C, and 55°C was used. The preliminary results of ddPCR condition
show that an optimized PCR annealing temperature of the assay was 55-56°C for Pfhrp2 and Pf tubulin, which
provided a clear separation between DNA positive and negative droplets. Further study, we will use the novel
developed ddPCR conditions for detection and quantification of Pfhrp2 gene using the clinical specimen in
Thailand. The prevalence of Pfhrp2 gene deletion will be analyzed.

Keywords: Plasmodium falciparum histidine-rich protein 2 (Pfhrp-2) gene deletion, droplet digital PCR
(ddPCR)

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Making perfect fungal slide by a novel and simple modified agar block smear technique

Natthaphum Saenkhampha1, Pattaraporn Pintawa1, Ariya Chindampor2, Navaporn Worasilchai1
1Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University
2Department of Microbiology, Faculty of Medicine, Chulalongkorn University

Accurate fungal identification is one of the key factors supporting patient management by selecting
the appropriate antifungal drugs. Presently, even a lot of novel technologies have been developed based on
molecular approach, cultivation together with conventional identification based on macroscopic and
microscopic remains the gold standard. To identify the genus and/or species of fungi, complete structure
under microscopy is very crucial. Crushed and/or damaged fungal structure in which often happen during the
process of hyphal teasing (lactophenol cotton blue wet mount technique) and/or alcohol rinsing (conventional
slide culture technique) leading to misidentification or failure of identification. To skip those processes, agar
block smear technique has been developed by using Acanthamoeba agar which is uncommon in mycology
laboratory. Thus, in this study we aim to modified the common fungal media for fungal microscopic study. To
prepare the fungal slide, nineteen strains of filamentous fungi: hyaline septate hyphae (n=12), non-septate
hyphae (n=2), dark septate hyphae (n=3), and thermal dimorphic fungi (n=2) and one strain of fungus-like
organism named pythium insidiosum were cultured on five extremely low-nutrition agar (1:2, 1:5, 1;10, 1:100,
and 1:1,000 water dilution of Sabouraud dextrose medium). The ideal medium for fungal slide preparation is
the one that is able to promote the growth of fungi in the submerged form. Based on the obtained result we
found that 1:10 water dilution of Sabouraud dextrose medium is the ideal one. To evaluate the capability of
this modified medium in fungal structure preservation, the genus and/or species identification among the
same strains obtained by three different methods: conventional slide culture, sequencing, and modified agar
block smear were compared. Among the recruited fungal strains that both genus and/or species were
identified by sequencing, four strain, classified as non-septate hyphae (n=2), dark septate hyphae (n=2) was
unable to be identified even in the genus level by conventional slide culture whereas all of them was able to
be identified, in the genus (n=2) and in the species level (n=2) using modified agar block smear. By
conventional slide culture method, it was shown that eight strains, classified as hyaline septate hyphae, was
able to be identified only in the genus level whereas seven of them was able to be identified in the species
level using modified agar block smear. In conclusion, modified agar block smear is one of the novel and
simple candidate techniques for fungal slide preparation which can be applied for all group of fungi.

Keywords: Fungal identification, Modified agar block smear, Slide culture

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Screening of fluconazole susceptibility of Candida spp. from a direct hemoculture using molecular
detection methods

Apinya Piapong, Sutinan Yokmanee, Panan Ratthawongjirakul
Program of Medical Technology, Faculty of Allied Health Sciences, Chulalongkorn University

The incidence of fungal infections has increased significantly. Candida spp. are the most common causes of fungal
infection. More than 90% of invasive infections are caused by Candida albicans, Candida glabrata, Candida
tropicalis, Candida parapsilosis, and Candida krusei. Fluconazole has been used as a drug of choice for the
systemic treatment of candidemia. However, some Candida spp. are intrinsic resistant to fluconazole that may
cause a treatment failure. Therefore, early identification of Candida spp. infection is essential for predicting the
species-specific primary resistance and variable antifungal susceptibility to influence therapeutic decisions.
Conventional methods for the identification of Candida spp. are based on morphological and biochemical test
attributes. Hence, several manuals and automated prompt commercial systems for identifying Candida spp. have
been developed; some of them may have significant sensitivity concerns. To overcome these limitations, newer
molecular techniques have been developed that allow accurate and rapid identification of Candida spp. The
objective of this review was to give an overview of the advantages and limitations of currently available molecular
methods for the diagnosis of invasive candidiasis.

Keywords: Candida spp. invasive candidiasis, fluconazole

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The characterization of Cyt2Aa toxin from Bacillus thuringiensis for the binding to various
cell membrane

Supparat Prasatchai, Anantaya Keawsakul, Anutida Singchalad, Chontida Tangsongcharoen
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha university

Cyt2Aa is the bacterial insecticidal proteins that is produced from Bacillus thuringiensis. It is toxic
to different species of mosquito larvae by acting pore-formation or detergent-like action to lipid membrane,
leading to larval death. In addition, the activated Cyt2Aa protein exhibits broadly cytolytic activity against
other cells. Previous studies revealed that Ile150 and Thr144 play a significant role for lipid membrane
binding and their binding capacity depends on the lipid composition and lipid fluidity. Thus, this study aims
to characterize Cyt2Aa proteins from wild-type and mutant proteins (I150K and T144A) for the binding to
bacteria in different strains. The results showed that both wild-type, mutant I150K and T144A proteins
exhibit the inhibitory activity on pathogenic strains of Gram-negative Escherichia coli and Salmonella spp.
And the Gram-positive Staphylococcus aureus. It seems likely that Cyt2Aa protein could inhibit the
bacterial growth of Gram-negative greater than Gram-positive bacteria. It implys that outer membrane
lipopolysaccharide of Gram-negative bacteria promotes the protein lipid membrane binding while thick
peptidoglycan of Gram-positive bacteria could affect to the Cyt2Aa protein binding. Moreover, the mutant
I150K exerts the anti-bacterial activity greater than mutant T144A. It might cause the interaction of mutants
with phosphoglycerol in bacteria. Overall, these results suggest that Cyt2Aa has antibacterial effect on
pathogenic species of bacteria.
Keyword : Bacillus thuringiensis, Cyt2Aa toxin, Bacterial inhibition activity

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Anti-bacterial Activity of local rice extracts of Sakaeo Province

Decha Wattanapraditchai , Vitsarut Surad , Suthiwat Charoenthawonwit , Tistaya Semangoen
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

This study is to study the effect of indigenous rice extracts on inhibiting pathogenic bacteria growth by

extracting cultivars of native brown rice in SaKaeo Province, namely KKK, KK, KD, F , N , N , and other common

types of rice and common rice varieties in the market for comparison testing, namely RD , KDML- by extracting

by ethanol alcohol, dried evaporated and tested for total phenolic compounds by Folin- Ciocalteu assay using

Gallic acid as the standard substance. The results showed that the mean total phenolic compounds were

, ,, , , , g GAE/mg crude extract,

respectively and testing for anthocyanin content by The pH-differential method showed that the average

anthocyanin content was , , , , , , ,

mg/L Equivalent to Cyanidin- glucoside. The antibacterial activity was tested by agar disc diffusion

method. The extracts were prepared at concentrations of , , , , and mg/ml. Tested on

bacteria with a dose adjusted equal to McFarland no. , which is S. aureus, S. agalactiae, S. pyogenes, E.

faecalis, E. coli, K. pneumonia, Salmonella spp, and Shigella spp. penicillin as the positive control. For gram-

positive bacteria, kanamycin was used as a positive control. For Gram-negative bacteria, DMSO was used as a

negative control. The experiment was repeated three times. The results showed that extracts from KKK, KK rice

strains were able to inhibit S. agalactiae. KKK rice at concentrations of and mg had inhibition zone of

, , respectively and KK rice at concentrations of and mg had inhibition zone of ,

KKK rice strains were tested for Minimal inhibitory concentration (MIC) and Minimum bactericidal

concentrate (MBC) with S. agalactiae. It was found that the MIC was mg/ml and MBC was mg/ml. This

study demonstrated that extracts from KKK and KK rice strains were able to inhibit S. agalactiae.

keywords Rice extracts, Anti-bacterial, Antioxidant Sakaeo Province Phenolic compound,
Anthocyanin

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Antibacterial activity of Artemisia lactiflora extract against Staphylococcus aureus

Jedsada Sunun, Pornanan Kuaekhai
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

Artemisia lactiflora or Jing Zhuhai is a plant that has many effects such as
alleviates liver fibrosis, anti-tumor, and also has been used in medicine for thousand years as a drug for
fevers, chills and a cold remedy for blood purification, detoxification. A. lactiflora has been found
Polyacetylene or artemisidiyne A. In this study, the Kirby-Bauer disc diffusion method was tested. The
purpose of this study was to study and test the antibacterial activity (Staphylococcus aureus) of A. lactiflora
extract. The purpose of this study was to test antibacterial activity of A. lactiflora extract with 8 different
concentrations diluted by 1 % DMSO that 1,600 µg/ µl, µg/ µl, µg/ µl, µg/ µl, µg/
µl, µg/ µl, µg/ µl and µg/20 µl by disc diffusion method, and penicillin was used as a positive
control. The results showed inhibition zone only 1,600 µg/ µl as mm. This study exhibited that A.
lactiflora extracts was able to inhibit S. aureus.
Keyword: Artemisia lactiflora, disc diffusion, Staphylococcus aureus, antibacterial activity

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Bioinformatics analysis of the Morganella morganii in response to cadmium toxicity based on
proteome data

Sirinthip Khlaychinda, Jarida Rakjareon and Patcharee Isarankura-Na-Ayudhya
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University

Cadmium (Cd) is a non-essential heavy metal, that toxic and harmful to many organisms and the
environment. At present, cadmium was used in agricultural and industrial development leads to a large amount
of cadmium contamination in the environment and accumulating in plants and animals with a long half-life.
Microorganisms found in soil and water are important components of the ecosystem as they play critical roles
in the earth's biochemical cycle and the biological activity of soil. Most of the bacteria attempted to adapt in the
contaminated environments. Morganella morganeii was reported to tolerate the cadmium ions by an unknown
mechanism. The purpose of this research was to find the main mechanism of cellular adaptation against a sub-
toxic dose of cadmium between Cd-tolerant stain (environmental and clinical isolates) and non-tolerant strain
(laboratory isolate) of Morganella morganeii by using bioinformatics tools and proteome data sets from
preliminary data. STRING (Version 11) and online databases were applied to analyze these proteins data sets,
by focusing on environmental isolate which is a species found mainly in the environment. Our comparative study
of protein expression in three isolates of Morganella morganeii revealed that environmental isolate had mainly
mechanisms to respond to cadmium, consisting of reduced uptake and efflux pump in order to reduce the
intracellular metal concentration. Other mechanisms were used to support this adaptation such as protein
synthesis and glycolysis pathway. Changes in proteins implicated in transportation are believed to be
accounted for cadmium efflux from the cells. This finding gains insights into the underlying fundamental data of
Morganella morganeii in natural adaptation under cadmium contaminated conditions, which can be applied in
the bioremediation of cadmium toxicity in the environment and understanding the microorganism community
for maintaining ecosystem stability in the environment.
Keywords: Morganella morganeii, bioinformatics, cadmium, proteomics, efflux pump

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Detection of Colistin gene and Carbapenemase gene in Extended Spectrum Beta Lactamase
producing Escherichia coli and Klebsiella pneumoniae

Anukrit To, Nantarat Klasuk and Worada Samosornsuk
Department of Medical technology, Faculty of Allied Health Sciences, Thammasat University

Gram negative bacteria are the most important pathogen that can produce Extended Spectrum Beta
Lactamase ESBL enzyme including multidrug resistant Escherichia coli and Klebsiella pneumoniae
Carbapenems and colistin are used to be drugs of choice for treatment resulting in resistant to these drugs
In this study, we examined carbapenems and colistin resistance genes in 175 strains of ESBL producing E coli
159 strains and K pneumoniae 16 strains The test methods were followed 1 Determination of carbapenems
MIC by automated model Phoenix M50 Becton Dickinson 2 Determination of colistin MIC using automated
model Sensititre Thermo Scientific and Broth microdilution CLSI 3 Detection of carbapenemase enzyme
using CarbaNP method 4 PCR assay for carbapenemase genes including blaKPC, blaNDM, blaVIM, and blaOXA 48
and colistin resistance genes including mcr 1, mcr 2, and mcr 3 Phenotypically, all strains didn t produce
carbapenemase enzyme and were susceptible to carbapenems Colistin resistant strains were found in 23
strains 14 5 of E coli MIC range 4 64 g mL and 2 strains 12 5 of K pneumoniae MIC 8 16
g mL For all carbapenems susceptible strains, carbapenemase genes were detected in 11 strains 6 3
including 9 strains of blaOXA 48 E coli, 1 strain of blaOXA 48 with blaNDM E coli and 1 strain of blaOXA 48
K pneumoniae In addition, colistin resistant genes were found in 14 strains 8 including 9 strains of mcr 1
E coli, 3 strains of mcr 3 E coli and 2 strains of mcr 3 K pneumoniae Moreover, 3 strains 1 9 of E coli
were found blaOXA 48 together with mcr 1 genes In conclusion, Carbapenems resistance genes blaOXA 48 and
blaNDM and Colistin resistance genes mcr 1 and mcr 3 could be examined in ESBL producing E coli and
K pneumoniae This study might be a guideline to select the appropriated antimicrobials for the patients
infected with ESBL producing E coli and K pneumoniae

Keywords Escherichia coli, Klebsiella pneumoniae, Extended Spectrum Beta Lactamase ESBL ,
Carbapenemase, Colistin

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