The 16th CU-TU MT Student's Research Conference

Optimization of Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for
discrimination of human steroid hormones isoforms analysis.

Jaruda Witoonsaridsin, Panisara Kaenkong, Siriporn Sangsuthum
Program of Medical Technology, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University

Human steroid hormones analysis plays an important role in diagnosis and monitoring of endocrine
disorders such as Cushing’s disease, renal failure, and congenital adrenal hyperplasia (CAH). Therefore, the
analysis of individual steroid hormone is medically important as it can help to understand the process and
severity of the diseases. The gold standard method for steroid hormones analysis is liquid chromatography-
tandem mass spectrometry (LC-MS/MS). Many studies using reverse phase column for steroid hormones
analysis. However, some groups of steroid hormones are challenging in separation using such stationary phase
including Aldosterone and Cortisone, Corticosterone and 11-Deoxycortisol and 21-Deoxycortisol, and
11-Deoxycorticosterone and 17-OH-Progesterone. Instead of reverse phase column, biphenyl column was also
used and gave effective result in separation of steroid hormones isoforms. However, the chromatography time
for steroid hormones analysis using biphenyl column was longer than C18 column. This study, biphenyl column
and C18 column will be used for steroid hormones isoforms analysis by LC-MS/MS. The optimization of
LC-MS/MS system for discrimination of 15 human steroid hormones will be targeted. Human steroid hormones
were extracted from serum using liquid-liquid extraction, then analyzed by LC-MS/MS using either C18 column
(Kinetex® 3.1x100 mm, 2.6 µm) or biphenyl column (Kinetex® 150x2.1 mm). Acetonitrile and water mobile
phase system were used for both columns. The expected result is biphenyl column could be used for steroid
hormone isoforms analysis with a shorter chromatography time than C18 column. Thus, this optimized
LC-MS/MS system would be a promising tool for MS based steroid assays including various isobaric
substances.

Keywords : Liquid chromatography with tandem mass spectrometry (LC-MS/MS), Biphenyl column,
C18 column, Steroid hormones

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Page 101

Hematological parameters in Sarcopenia elderly: Systematic Review and Meta-Analysis

Apichaya Yartsamer, Jutarmard Juntartip and Khaimuk Changsri
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University

Sarcopenia is a progressive and generalised skeletal muscle disorder commonly found in elderly that
is associated with increased adverse outcomes which affects quality of life. When sickness occurs,
hematological values may change and indicate physiological abnormalities in the body. We expect the
association of hematological parameters and sarcopenia to be explored. The purposes of this study were to
show hematological values of the elderly patient that compare between sarcopenia group and non-sarcopenia
group and to determine the hematological value that can classify the severity of sarcopenia. We systematically
searched for published articles from accessible databases such as Medline, ScienceDirect and Springer in
2016 to present and also an unpublish data from the specific sarcopenia field. We assessed the research
using Newcastle Ottawa quality assessment scale. Meta-analysis of eligible studies were performed using
Meta-mar program (Meta-Mar free online meta-analysis service) by choosing random effect model. A total of
1,587 articles were initially identified, then after Preferred Reporting Items for Systematic Reviews and Meta-
Analyses (PRISMA) was applied 17 articles were included in this study. The results revealed that the most
commonly hematological parameters reported to show differences between sarcopenia group and non-
sarcopenia group were hemoglobin level, white blood cell count, and platelet to lymphocyte ratio (PLR). The
forest plot of mean differences of hemoglobin level (SMD= -1.67, 95%CI: -2.655, -0.692, P<0.001, I2=99.1%,
Tau2=3.209) showed clearly different, while white blood cell count (SMD= 3.28, 95%CI: -0.210, 6.774, P=0.065,
I2=99.7%, Tau2=12.636) and PLR (SMD= 0.07, 95%CI: -0.271,0.421, P=0.671, I2=46.7%, Tau2=0.045) were not
clearly different. In conclusion, the hematological values such as hemoglobin level, white blood cell count and
PLR may not use for identifying sarcopenia elderly form non-sarcopenia elderly but may be used for follow up
the occurrence of sarcopenia in elderly patients.

Keywords: Elderly, Sarcopenia, Hematology

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Page 102

Updated prevalence of thalassemia in Thailand

Kunlanat Yimnoi, Kenika Sukrong, Kamonlak Leecharoenkiat
Programs of Medical Technology, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn
University

Thalassemia is a hereditary blood disorder that has become a major public health issue in Thailand.
The thalassemia genes are widely distributed in Southeast asia region. The prevalence of thalassemia in
Thailand had been reported for many decades however the comprehensive prevalence data is need to be
updated. A systematic review of the prevalence of thalassemia in Thailand published from year 2011 to 2021
were analyzed. Of this, the prevalence of thalassemia analyzed by this current study tends to be lower than in
previous studies. The thalassemia traits was found in range 7.17 % to 31.58 %, while Hemoglobin H
disease was found in 0.15 % to 1.23%. The 3.7 deletion type was revealed to be the most common
thalassemia genotype detected in the Thai population, followed by the SEA type. The prevalence of
thalassemia, Hemoglobin H disease was ranged from 1.62 % to 2.86%, and -thalassemia with Hemoglobin
E disease was detected in 0.06% to 0.14%. This study gives an update on the prevalence of thalassemia in
Thai population. The thalassemia is still prevalent in the community, an efficient thalassemia prevention and
control program should be maintained in Thailand.
Keywords: prevalence, thalassemia, -thalassemia, -thalassemia

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Page 103

Anti-leukemic activity of rutin on human acute lymphoblastic cells.

Jantima Nanongkhai, Niparat Seehabut, Supantitra Chanprasert
Program of Medical Technology, Faculty of Allied Health Sciences, Chulalongkorn University

Background: Rutin is a citrus flavonoid glycoside found in many plants. It has various effects including anti-
inflammation, neuroprotection and anti-cancer. Currently, there are few studies involving anti-leukemic activity
of rutin. However, mechanism of rutin mediated apoptosis of leukemia cells remain unclear.
Objective: We aimed to investigate whether rutin has anti-leukemic effects and induce apoptosis in human acute T
lymphoblastic cells (Molt-4).
Methods: In cytotoxic testing, using trypan blue exclusion method and microscopic analysis. Apoptotic induction
was studied by annexin V and PI staining and apoptotic cells were determined by flow cytometer.
Results: We found that rutin inhibited Molt-4 cell growth with IC50 values of 217.12 µM.Also, by microscopic analysis,
blebbing pattern of cell apoptosis was found in cells treated with rutin (130 µM) for 48 hours. In order to confirm
apoptotic activity of rutin, we plan to analyze apoptotic cells by flow cytometry, and we expect rutin to induce Molt-
4 cell death through apoptosis.
Conclusion: Our preliminary results indicated that rutin has anti-leukemic activity mediated apoptosis. However,
further study is certainly needed.
Keywords: Rutin, Acute Lymphoblastic Leukemia (ALL), Molt-4, Apoptosis

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Page 104

The in vitro anticoagulant effect of red rice bran extract on human blood

Chonlada Chueameerang1, Kamollux Kumwang1, Sudarat Phanphong1, Nunthawadee Niamnuy1, Narongsuk Munkong2,
Kevalin Vongthoung1
1Deparment of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University
2Deparment of Pathology, Faculty of Medicine, University of Phayao

Blood coagulation is a rapid and proficient process that produces clot formation and requires
regulation Blood coagulation disorder is a feature of many diseases The present study investigated the in vitro
effects of red rice bran extract on blood coagulation of healthy human volunteers Oryza sativa L was extracted
with 50 methanol and the concentrated crude extract was subjected to phosphate buffered saline Varying
extract concentrations 5, 10, and 20 mg mL were tested in vitro on blood coagulation profiles, including
prothrombin time PT and activated partial thromboplastin time aPTT of healthy human volunteers Oryza
sativa L ethanol extract was found to prolong the prothrombin and activated partial thromboplastin times in the
blood obtained from the volunteers Red rice bran extract at a concentration of 5 mg mL represents PT and
aPTT of 26 53 and 121 23 seconds, respectively, while 10 mg mL represents PT and aPTT 39 77 and 164 20
seconds, respectively, and 20 mg mL represents PT and aPTT 67 30 and 174 90 seconds, respectively The
highest prolongation effect was recorded at a concentration of 20 mg mL These results show that Oryza sativa
L possesses anticoagulant properties which may be exploited in the treatment of blood coagulation disorders
Keywords Red rice bran extract, coagulation, anticoagulant, prothrombin time, activated partial thromboplastin
time

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Page 105

CU Parasite E-learning for practicing on anatomy of Nematode, Trematode and Cestode

Nusara Pusomta, Penyupha Woraditsakulchai, Attakorn Palasuwan, Duangdao Palasuwan
Department of Medical technology, Faculty of allied Health Sciences, Chulalongkorn university

Since the epidemic situation of COVID-19 all teaching class including Clinical Parasitology Laboratory have
been transformed to online platform. Medical technology student would not allowed to practice in the laboratory.

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platform for practicing on anatomy of Nematode, Trematode and Cestode for students to compare the development
of human parasite shapes. Sixty Chulalongkorn University's 3rd year medical technology students were recruited
in this study. A group of volunteers (30 students), studied online platform and CU Parasite E-learning, were
compared to a control group who studied regular online platform from instructors only (30 students). This
comparative study was conducted while Clinical Parasitology Laboratory was taught during September-October
2021.The learning scores between pre-test and post-test from 2 groups were compared. This study showed that
the learning scores were significantly increased (P<0.001) in both groups regardless the multimedia tools. However,
the average scores of multimedia group had significantly higher compared to non-media group (interaction effect
between media vs. classical learning, P<0.001). We concluded that CU parasite E-learning media could contribute
to the development of better teaching of parasitology and this study suggests that the use of CU parasite E-learning
media should be further developed to support the teaching and learning of Clinical Parasitology Laboratory subjects
in the COVID-19 situation.

Keywords : Coronavirus disease (COVID-19), Chulalongkorn University (CU), Electronic Learning (E-learning)

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Page 106

Hemolytic effects of herbs in G6PD-deficient erythrocytes

Napassorn Satayapat, Pimsiri Onsaad, Duangdao Palasuwan
Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University

Glucose-6-phosphate dehydrogenase (G6PD) is a very important enzyme that regulates various
biochemical reactions in the body. It’s also responsible for keeping red blood cells function properly and live a
normal life span. G6PD enzyme is an inherited condition. It’s affecting more than 400 million people worldwide.
G6PD enzyme helps red blood cells work correctly. A lack of this enzyme can cause hemolytic anemia. Most
persons with G6PD are asymptomatic but exposure to oxidant drugs and certain substances may induce
hemolysis. However, the association between herbal and hemolysis is less clear. The objective of this study
was to evaluate the association between herbal and hemolytic effects in G6PD deficient individuals.
We searched 3 electronic databases from July until December 2021 for articles describing the use of herbal in
G6PD deficient individuals. All study designs were included as long as they contained clinical information.
These gathered findings were summarized narratively. Four publications met inclusion criteria. These reported
on 4 herbal. Overall evidence linking hemolysis to herbs were found for Moringa (Moringa oleifera Lam.) and
Ginkgo (Ginkgo biloba L.). No evidence of harm was observed for Huanglian (oral administered). The extract
of Cornflower (Centaurea cyanus L.) does not induce hemolysis. The reviews showed that the extract from
Moringa leaves has a stimulating effect on hemolysis of red blood cells in G6PD deficiency individual, which
occur only in condition with high oxidization but has no effects on hemolysis of normal red blood cells. Huanglian
is safe to be consumed oral administered in appropriate dosage. However, the intravenous and intraperitoneal
injections is not recommended. In general, Ginkgo does not cause hemolysis. But in some cases, the temporal
relationship between acute hemolysis and Ginkgo injection has been found. Therefore, more studies on
mechanistic effects of the Ginkgo should be performed in vivo to evaluate the safe and proper of use.

Keywords: glucose-6-phosphate dehydrogenase (G6PD), Moringa oleifera Lam., Rhizoma Coptidis, Ginkgo
biloba L., Centaurea cyanus L.

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Page 107

Effect of Pinocembrin on the level secretion of Interleukin-2 (IL-2) in T-cell lymphoblastic leukemia
(T-ALL)

Nichapat Wiangha1, Akira Wongvichien1, Sirikalaya Brimson1
1Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn University

T-cell lymphoblastic leukemia (T-ALL) is a type of acute leukemia (ALL) which is rapidly progressive and
aggressive. Normally, Adult and/or adolescent patients respond to current treatments. But some patients have
leukemia relapse after treatments which is a major therapeutic problem. Interleukin-2 (IL-2) is identified as T-cell
growth factor which is necessary for the growth, proliferation, and differentiation of naïve T cells into effector T cells.
In this study, Jurkat cells were used representing leukemic T-call. As IL-2 bound to IL-2 receptor on Jurkat cell
surface, it affects T-cell leukemia cells to overgrowth and over-proliferate. Over produce of IL-2 then causes
Leukemic T cell cancer. Pinocembrin is one of primary flavonoids isolated from Honey, Pinus heartwood,
Eucalyptus, Propolis, etc. It is a major flavonoid molecule with multiple usages in the pharmaceutical industry. Its
vast ranges of pharmacological properties have been discovered including anti-allergic agent, anti-microbial, anti-
inflammatory, antioxidant, and anti-cancer.

The objective of this study is to investigate the effect of Pinocembrin on the secretion level of IL-2 in T-cell
lymphoblastic leukemia (T-ALL). Jurkat (clone E6-1) representing T-cell lymphoblastic leukemia (T-ALL) are treated
with Pinocembrin (200 -800 µM) or without Pinocembrin as controls in 5% CO2 and 37๐C for 48 hours. The level
secretion of IL-2 is measured using ELISA assay. Finally, level of IL-2 is also normalized using total protein content
in each sample measured by Bradford protein assay. The IL-2 level will be analyzed statistically after three
independent experiments using One-way ANOVA. Due to anticancer property, we expect that Pinocembrin could
reduce the level secretion of IL-2 resulting in inhibiting cell growth and proliferation in does dependent manners.
Pinocembrin could cause apoptosis in Jurkat cells in high does in which further studies will be needed. Moreover,
the pinocembrin effects and usages may provide alternative treatments for leukemia.

Keywords: T-cell Acute lymphoblastic leukemia (T-ALL), Interleukin-2 (IL-2), Pinocembrin, ELISA

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Page 108

Effect of Pinocembrin on the secretion of pro-inflammatory and apoptotic cytokine; Tumor
necrosis factor (TNF-α) in Jurkat cells

Kanjanaphan Santiworanart, Napreechaya Mogmued, Sirikalaya Brimson
Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University

T-cells acute lymphoblastic leukemia (T-ALL) causes 25 percent of ALL cases in adults. T-ALL could be
diagnosed with cell morphology. This causes many immature white blood cells, known as T-cell lymphoblasts
in the bloodstream and the bone marrow. Immunophenotyping is useful in T-cell lineage classification which
expresses CD3 T-cell marker. The diagnosis with various methods could evaluate the prognosis and
approach appropriate treatment.

In this research, we study on the effect of pinocembrin on the secretion of pro-inflammatory and apoptotic
cytokine, known as tumor necrosis factor (TNF-α). We notice that TNF-α affect Jurkat cells (T-cells acute
lymphoid leukemia (T-ALL) which represent T-cells acute lymphoid leukemia (T-ALL). According to the
previous research, we found that pinocembrin could activate apoptosis of cancer cells. Therefore,
pinocembrin could inhibit cancer cells.

In the method,first, prepare % Cell viability of Jurkat cells with Trypan blue assay. Notice that % Cell
viability should not be lower than 95%. After that, culture Jurkat cells on 24 well plates and bring Jurkat cells
to test with pinocembrin solution at the concentration of 200, 450, 600, 800, uM. Use Dimethyl sulfoxide
(DMSO) as the solvent at a concentration lower than 1% is the control of the test. Then bring treated Jurkat
cells to incubate at 37℃ and 5% CO2 for 24 hours. Then, add PMA activator at the concentration of 12.5
ng/mL and PHA activator at the concentration of 0.25 ug/mL. Incubate treated cells at 37℃ and 5% CO2 for
12 hours Collect the supernatant of culture media to measure TNF-α level with ELISA assay. Perform the
triplicate test. (3 independent experiments) Finally, analyse TNF-α cytokine levels with One-way ANOVA
which is the statistical method showing correlative outcomes.

Our expected result is that Pinocembrin in different concentrations could deduct Jurkat cells’ TNF-α cytokine
levels. Quantity of TNF-α could reverse with Pinocembrin with highest concentration which stimulate the cell
secrete lowest quantity of TNF-α.

Keywords: T-cells acute lymphoblastic leukemia (T-ALL), Jurkat cells, Pinocembrin, Tumor necrosis factor
(TNF-α), enzyme-linked immunosorbent assay (ELISA)

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Page 109

Identification of ETV6 RUNX1 like subtype in children with B cell precursor acute lymphoblastic
leukemia

Khullakan Phorban, Titirat Namkang, Ekchon Mukda
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University

B cell precursor acute lymphoblastic leukemia BCP ALL is the most common malignancy in children
However, 25 of pediatric patients with BCP ALL still lack defined genetic hallmarks and will be classified as
B ALL other The objective of this study is to identification of ETV6 RUNX1 like subtype and gene expression
profiles in children with B other ALL patients no classifiable by conventional methods We performed a
microarray based gene expression analysis of leukemic cells from patients 15 cases The results showed that
no ETV6 RUNX1 like subtype found in our study because of case number limitation However, our finding
demonstrates genetic up regulations in B other standard risk ALL patients and FLT3 6 7, 86 is the most
common However, TOX, EPOR, CCL17, SFPQ, NRAS, SPTAN1 and TBL1XR1 showed down regulation with
approximately 71 5 7 , each Genetic aberration in B other high risk ALL patients had also identified The
results showed that IKZF1 7 8, 88 down regulation is the most frequent, followed by TOX and TMEM2 6 8,
75 , FLT3, EPOR, MSH6 and BLNK 5 8, 62 The gene expression profiles of leukemic cells are associated
with JAK family activating, ABL class, Ras signaling, and other transcriptional factor or kinase activity which
were 11 9 , 5 8 , 4 3 and 78 , respectively Our study provided an expression profile of childhood B ALL
others that might be helpful for diagnosis and prognosis, and we also identified candidate genes that might be
used for further study of BCP ALL pathogenesis

Keywords acute lymphoblastic leukemia, B others ALL, genetic abnormalities, microarray

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Page 110

Study on the correlation between SNPs on DNA mismatch repair gene and Signaling pathway
gene with colorectal cancer risk in Thais in the Lower Northeastern Region

Waranya Suntornnon, Worasiri Rungrojchaiyaporn and Malinee Pongsavee
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University

Colorectal cancer CRC is the third most common cancer in men worldwide including Thailand
causes of 1 in 5 cancer deaths Single Nucleotide Polymorphisms SNPs can affect gene expression as well
as protein quantity and function depends on the locations of SNPs on DNA strands SNPs that are related with
colorectal cancer as follows MLH1 rs1799977, MLH1 rs1800734, MSH3 rs26279, MSH6 rs3136228 and
SMAD7 rs12953717 This study aims to investigate the correlation between SNPs on DNA mismatch repair
gene and signaling pathway gene with colorectal cancer risk in Thais in the Lower Northeastern Region
One hundred fifty one samples from colorectal cancer patients and one hundred samples from healthy controls
were analyzed Genomic DNA was extracted from white blood cells and the real time polymerase chain reaction
technique qPCR was used to demonstrate genetic polymorphism of MLH1 rs1799977, MLH1 rs1800734,
MSH3 rs26279, MSH6 rs3136228 and SMAD7 rs12953717 in all samples The results showed that the MLH1
rs1799977 homozygous variant GG genotype was associated with an increased colorectal cancer risk to 6 09
folds OR 6 09 , 95 CI 0 32 114 44 , MLH1 rs1800734 homozygous variant AA genotype was associated
with an increased colorectal cancer risk to 1 36 folds OR 1 36 , 95 CI 0 63 2 94 , MSH3 rs26279
homozygous variant GG genotype was associated with a protective factor OR 0 09 , 95 CI 0 00 1 80 ,
MSH6 rs3136228 heterozygous variant TG genotype was associated with an increased colorectal cancer risk
to 1 49 folds OR 1 49 , 95 CI 0 50 4 43 and SMAD7 rs12953717 homozygous variant TT genotype was
associated with an increased colorectal cancer risk to 1 67 folds OR 1 67 , 95 CI 0 80 3 38 but
statistically significant were not found This study demonstrated MLH1 rs1799977 was associated with an
increased colorectal cancer risk in Thais in the Lower Northeastern Region more than other SNPs

Keywords MLH1 rs1799977, MLH1 rs1800734, MSH3 rs26279, MSH6 rs3136228, SMAD7 rs12953717,
Colorectal cancer

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Page 111

Correlation of dysmorphic red blood cells and hematuria for the prognosis of glomerulonephritis

Pawanrat Lansretta, Thidarat Chanpong, Pongpera Rattanaliam, Chollanot Kaset and Sirinart Chomean
Department of Medical technology, Faculty of Allied Health Sciences, Thammasat University

The occurrence of hematuria is associated with glomerulonephritis Patients with glomerulonephritis
presents anemia, fatigue, arthritis and can eventually lead to kidney failure Early diagnosis is important to
properly treatment and prevent kidney failure However, the definite diagnosis requires tissue biopsy which is
invasive and expensive A study of red blood cell RBC morphology or dysmorphic RBCs dRBCs can be
helpful for identifying the glomerulonephritis which can improve the effectiveness of routine urinalysis In this
study, we analyzed and identified RBC and dRBCs according to Nagahama criteria from 100 hematuria
samples A receiver operating characteristic ROC curve was used to illustrate the diagnosis ability of
glomerulonephritis Results showed that the identification of dRBCs increases the specificity to diagnosis of
glomerulonephritis under the optimal cut off value More than 63 of dRBCs provides the 41 sensitivity and
77 specificity Moreover, the presence of dRBCs D1 more than 1 5 showed the 32 sensitivity and 82
specificity The sensitivity and specificity of D2 >5 3 was 38 and 73 , respectively Also, D3 >38 had
76 sensitivity and 47 specificity The results can be concluded that the identification of dRBCs can be used
as a marker for prognosis of glomerulonephritis Since, it gives a high specificity which can be improved the
effectiveness of routine urinalysis

Keywords Hematuria, Glomerulonephritis, Non glomerulonephritis, Dysmorphic red blood cells, Routine
urinalysis

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Page 112

Assessment of coagulopathy for effectively evaluate severity of patients with COVID-19 infection:
A systematic review

Rungthiwa Prathumwan, Nutdanai Deejaroen and Wimolpak Sriwai
Department of Medical technology, Faculty of Allied Health Sciences, Thammasat University

Patients with COVID-19 infection can develop a coagulopathy, especially those with moderate to
severe disease. The pathogenesis of COVID-induced coagulopathy is not fully understood yet. In order to
prognosis of COVID-19 patients with coagulopathy, laboratory examination is used to identify coagulopathy.
However, it remains unknown whether the laboratory examination can be used in order to predict the degree of
coagulopathy or outcome. A systematic review was performed within PubMed and CINAHL databases between
January 2020 to August 2021 using keywords and variants or derivatives related to COVID-19, coagulopathy
and disease severity. The endpoint was the coagulation parameters (D-dimer values, platelet count,
prothrombin time, activated partial thromboplastin time) between non-severe vs severe disease and survivors
vs non-survivors. Furthermore, results on other coagulation factors (thrombin, fibrin, plasminogen activator
inhibitor 2) were also analyzed. The analysis showed that platelet count was significantly decreased, while D-
dimer, activated partial thromboplastin time, prothrombin time and C-reactive protein were significantly
increased in non-survivors than in those survivors. This data suggests a possible application of coagulable and
fibrinolytic profiles to assess the severity of COVID-induced systemic coagulopathy and decide on the
appropriate management.
Keyword: COVID-19, Coagulopathy, Severity

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Page 113

An in vitro study for hemostatic property of oxidized bacterial cellulose

Nirawan Wasukree, Supapit Areerob, Yaneenart Suwanwong
Program of Medical Technology (or Department of Medical Technology), Faculty of Allied Health Sciences, Chulalongkorn
University

Wound dressing materials is an important part of wound care .Normally,when we loss blood our body
go through a process called hemostasis. It is a process of slowing and stopping the flow of blood to initiate
wound healing. Hemostasis is insufficient in people who have a blood coagulation disorder .It is possibly life-
threatening. Nowadays,there are numerous materials available that can be used to make wound dressing. The
most common wound dressing materials, such as gauze, are made of oxidized cellulose. Although it is made
from plant cellulose, it still contains contaminants. In recent years, bacterial cellulose has been highlighted in
many reviews as a materials for wound dressing because of its beneficial including high purity
,biocompatibility,high water retention. Moreover,It can accelerate process of epithelialization that can be utilized
in several applications. BC is produced extracellularly by the gram-negative bacterial in Acetobacter genus.
In this study, we aim to study the properties of OBC as a hemostatic material. The objective of this study is
to study the effect of OBC on whole blood coagulation and platelet aggregation. PRP was prepared from 30
healthy subjects then applied to whole blood clotting assay and platelet aggregation test. The addition of OBC
was expected to result in decreased whole blood clotting time and increased platelet aggregation compared
to no addition of OBC.

Keywords: Wound dressing ,Bacterial cellulose(BC),Platelet rich plasma (PRP),Coagulation

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Page 114

Review: Anti-cancer activity of Quercetin on human leukemic cells

Donlawat Wasuanan1, Panithi Ronkhao1, Paweena Pradniwat2

1Program of Medical Technology, Faculty of Allied Health Sciences, Chulalongkorn University
2Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University

Acute myeloid leukemia (AML) is an aggressive hematological malignancy characterized by highly
proliferative accumulation of immature and dysfunctional myeloid cells. The cure for acute myeloid leukemia
(AML) remains a challenge for hematologists worldwide as the outcomes for patients with many leukemia
variants continue to be poor. This scenario indicates the increasing demand for innovative drugs and rational
combinative therapies. Among many kinds of treatments, the conventional methods such as chemotherapy,
radiotherapy, stem cell transplantation, etc., inducing many severe adverse effects, herbal therapy becomes
better accepted, owing to its anti-cancer property and ability to enhance human body's equilibrium with minimal
side effects. Various fruits and plants have been presented as promising sources of natural anticancer agents.
Many of these plant extracts are currently used in cancer therapy and prevention. Flavonoids are ubiquitous
groups of polyphenolic compounds present in most natural products and plants. These substances have been
shown to have promising chemo-preventive and chemotherapeutic properties with multiple target interactions
and multiple pathway regulations against various human cancers. Polyphenolic flavonoid compounds can block
the initiation or reverse the promotion stage of multistep carcinogenesis. Quercetin is one of the most abundant
flavonoids found in fruits and vegetables and has been shown to have multiple properties capable of reducing
cell growth in cancer cells. This review paper will particularly explore and emphasize on quercetin from herbs
and fruits used in the treatment of the acute myeloid leukemia.

Keywords: Acute myeloid leukemia ( AML) , Quercetin , Anti-cancer , Plant extracts , Cancer thetapy

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Page 115

Oxidative stress and antioxidant responses in elderly with anemia and thalassemia carriers

Chalisa Yeunyong, Anuphap Suriyawan, Wanwanuch Luecha, Sanita Singsanan*
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

Oxidative stress is an imbalance of free radicals and antioxidants in the body. The body’s biological
system can detoxify these reactive products. In elderly, oxidative stress has been reported to increase with a
decreasing in antioxidants. Aging can lead to oxidative stress and red blood cells death which cause anemia in
the elderly. As well as, thalassemia carriers are common in Thailand which associated with the oxidative damage
observed in erythrocytes due to the presence of excess globin chains. However, the results of various studies that
investigated oxidative stress and antioxidant status in elderly are still controversial. The objective of this study was
to determine whether the oxidative stress and antioxidant status in elderly with anemia and thalassemia carriers
are altered. All participants were 382 healthy elderly subjects. They were measured the level of lipid peroxidation
expressed as Malondialdehyde (MDA) by Thiobarbituric Acid-Reactive Substances Assay (TBARS) and
antioxidant status expressed that consisted of total antioxidant capacity and Catalase by Ferric Reducing
Antioxidant Power (FRAP) assay and Catalase activity assay. The results demonstrated that the levels of MDA,
FRAP and CAT were 0.67 µM, 570.23 µM, and 122.28 kU/L, respectively. There was no significant difference
between oxidative stress and antioxidant status in elderly with anemia and thalassemia carriers. However, healthy
older do not appear to be exposed to an oxidative stress but there was a decline of antioxidant status.

Keywords: Oxidative stress, Antioxidant status, elderly, anemia, thalassemia

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Page 116

The Study and Development of White Blood Cell Differential Application

Paritporn Jakkaichawadol, Araya Thaipetkun, Tanasan Sirirat
Huachiew Chalermprakiet University

In teaching hematology, White blood cell differential is taught through microscopic practices, which
can only be practiced within the class period. Therefore, researchers are interested in creating a White blood
cell differential application to practice outside the classroom. It can be practiced anywhere anytime which may
enhance the skills of users. By comparing the results of cognitive evaluation before and after using an
application among 50 4th year students of the Faculty of Medical Technology, Huachiew Chalermprakiat
University, it was found that cognitive evaluations before and after application usage were 3.66 and 4.44
respectively. The use of knowledge before and after using the application was 3.63 and 4.41 respectively.
Comparing the results of the assessment by using paired t-test statistics, it was found that after using the
application there was a statistically significantly higher assessment score than before using application (p-value
< 0.05). In addition, it was found that function, application design, content and utilization are 4.27,4.40,4.19 and
4.41 respectively. In conclusion, the application can aid white blood cell differential. It is convenient and
practical which enhances the skills of the user.

Keywords: White blood cell differential, application, enhances skills

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Page 118

Extraction collagen from tilapia fish (Oreochromis niloticus) scale for tissue engineering

Thanakorn Luangpaiboonsri 1, Sasikarn Chanthanuwutkul 2, Supanich Kongsin 3, Chirapond Chonanant
1Program of Medical Technology, Faculty of Allied Health Sciences, Burapha University

This study aimed to extract collagen from the scale of Oreochromis niloticus by acetic acid. The extracted
collagen was acid-soluble collagen (ASC) with appearance was white and fine powder. It was found that 0.42 grams
dry weight of extracted collagen were obtained from 100 grams dry weight of Tilapia fish scale and estimated %
yield collagen was 0.42%. Analyzed the properties of extracted collagen by UV-vis spectroscopy found that It was
high absorbance at 200-220 nm. which attributed to C=O, -COOH, CONH2. The absorbance can be found in
polypeptide of collagen this is part of triple helix structure of collagen. And found the spectra of Amide A, Amide
B, Amide I (C=O), Amide II, C-O-C stretching with PO2 stretching and Amide III (N-H) via FTIR analysis. As a result
this extracted collagen was protein and collagen type I. In summary, This extracted collagen is a source of collagen
is able to applied to Fabrication the scaffold in the future.
Keywords : Acid-soluble collagen (ASC), Fabrication the scaffold, tilapia fish scale, FTIR, UV-vis spectroscopy

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Page 119

Role of Durio zibethinus (durian rind extract) for developing bioactive compounds

Kunita Wonganu, Pimmada Tesana, Tamonwan Saykoon, Chontida Tangsongcharoen
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha university

Durian has been recognized as “ the king of fruits “ . It is the most popular of economic tropical fruits
in Thailand. Apart from the consuming durian in Thailand, the export of Thai durian have been increasing
consistently because of a strong demand from other countries. Many durian rind become the wastes. Thus, it
is certainly important to reduce these durian wastes by transforming into a renew product. Previous studies
showed that many fruit peels have many pharmacological activity such as anti-oxidant, anti-inflammation.
Therefore, this study aims to study the activity of durian rind extracts on bacteria and human cancer cells. The
compounds of durian rinds were extract by varying concentration of ethanol (0%, 25%, 50%, 75% and 100%
EtOH) as solvent. After lyophilization process, their durian powder in each solvent were dissolved with media
into different concentration for testing anti-cancer and anti-bacterial activity. The results of MTT assay showed
that 50% and 100% EtOH of durian rind extracts at 4 mg/ml could significantly decrease the cell viability of
Hct116 cells and also reduce cell viability of HepG2 cells. Moreover, anti-bacterial activity by using broth
dilution method showed that 50% and 100% EtOH of durian rind extracts at 20, 10 and 5 mg/ml were able to
inhibit the bacterial growth of S.aureus, E.coli and Salmonella spp. This study indicated that durian rind
extracted with 50% and 100% EtOH would contain the important compounds to inhibit the growth of bacteria
and cancer cells. In the future, durian rinds were extract would be developed as anticancer agents or
antibacterial agents on skin infection for the alternative medical therapeutics.
Keywords: Durian rind extracts, Antibacterial activity, Anti-cancer activity

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Page 120

Physical and chemical contamination and biological hazard in fresh seafood from the local fish
market in Chonburi province

Thanaporn Kumsing1, Chonlada Nopsophon1, Kanokporn Srisucharitpanit
1Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University

Safety assessment including physical, chemical and biological hazard of fresh seafood from Chonburi
market was examined. A total of 11 species, Rastrelliger brachysoma, Portunus pelagicus, Loligo duvauceli,
Cynoglossus macrolepidotus, Liza vaigiensis, Sardinella gibbose, Rastrelliger kanagurta, Alectis indicus, Gerres,
Siganus javus, and Eubleekeria splendens, of fresh seafood were collected from the 3 local fish market as
Won beach, Laem taen, and Ang sila. Microplastic, formalin, Anisakis spp. and Vibrio spp. were determined.
The results showed that the detection of microplastic and Anisakis spp. were found to be 90% and 10%,
respectively. Formalin was not found in any of the samples studied. Most microplastic contamination consisted of
mixing types such as fibers, fragments, and irregular shape. In addition, Anisakis spp. larvae were isolated from
Liza vaigiensis. However, morphology of the larvae is not sufficient to justify. DNA of Anisakis spp. positive sample
were extracted to identify and confirm by using PCR method is needed for further study.

Keywords: food contamination, microplastic, formalin, Anisakis spp., Vibrio spp.

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Page 121

Study of Saccharum sinense Roxb. extract against anti-oxidant and anti-microbial activities.

Nattanan Chuchuenbun, Phakphirom Phomlumphak, Rattiyakun Bunchan, Niramon Thamwiriyasati*
Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University
* Corresponding author: [email protected]

Black Sugarcane (Saccharum sinense Roxb.) has been used for traditional herbal medicine, which
contains polyphenols and alkaloids compounds for providing health benefits. There are some reports about black
sugarcane have low-sugar and low-calorie sweetened beverage. Intake of black sugarcane by elderly people and
DM patients are benefit for health outcome. However, these evidences support still unclear. This study aims to
extract the black sugarcane with water and ethanol, and test for their nutrient compound such as sugar, protein and
calcium. Aqueous and ethanolic extracts of two sugarcane species (sugarcane and black sugarcane) were
evaluated for their antioxidant and antimicrobial activity against gastrointestinal tract pathogens. The results show
that the measurement of glucose by black sugarcane and sugarcane extracts was a concentration of 20.28 mg/dL,
10.32 mg/dL, respectively. The total calcium of black sugarcane and sugarcane extracts measured by
Cresolpthalein Complexone method was a concentration of 1.297 mg/dL, 0.519 mg/dL, respectively. The
antioxidant activity was measured by Ferric Reducing Antioxidant Power assay (FRAP). FRAP value of Sugarcane
juice was 893.15 g/dL, which was higher than its FRAP value of black sugarcane juice as 410.08 g/dL. FRAP value
of Sugarcane bagasse extract was g/dL, which was higher than its FRAP value of black sugarcane bagasse
as 618.54 g/dL. The result of antimicrobial activity against seven pathogens, i.e., Escherichia coli, Pseudomonas
aeruginosa, Salmonella Group B, Shigella dysenteriae, Aeromonas hydrophila, Plesiomonas shigellodies and
Staphylococcus aureus was determined by agar disc/ well diffusion method with concentration of 0.3 g/ml. Their
microbial stains have no inhibition zone occur. While, the inhibition zone of the positive control as Ciprofloxacin
reached 31 mm., and the negative control distilled water showed no inhibition zone. These results suggest that
based on nutrient and variety of sugarcane extracts could lead to further gain in selective sugarcanes consumption.
Consequently, the method of processing on the extract enhances bioactive and value of products that could
improve protective consequence directly on health and goal of treatment.

Keywords: Antioxidant, Antimicrobial, Black Sugarcane, Saccharum sinense, Natural herb extract

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Page 122

Effect of Phyllanthus amarus Schumach. & Thonn. on Hydrogen peroxide-induced oxidative
stress in the human Keratinocyte

Sujinan Kwankaew , Kwanruethai Amnuaikarn , Suttida Anuson , Phornpun Phokrai
Department of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University

Phyllanthus amarus Schumach. & Thonn. is a tropical plant found in Southeast Asia, including
Thailand. Many studies report that it has many chemical properties such as glycosides, saponins, tannins
and flavonoids, which are phytochemicals as antioxidants. In Thailand, P. amarus, also called “Look Tai Bai”,
is well known as a traditional herbal for various physiological treatments including diabetes, and liver disease.
Free radical, which are produced both inside the body and in the environment, can cause the majority of
many diseases. Free radical containing oxygen molecules, defined as oxidative stress, can destroy many cell
types, including skin cell damage. This study aimed to investigate the protective effect of P. amarus extract
on keratinocyte HaCat cells. Cells were treated with P. amarus extract 10 – 200 µg/ml for 24 hours and
cell viability was evaluated by using MTT colorimetric assay. The suitable concentration was examined
for its protective effect on Hydrogen Peroxide-induced oxidative stress. This study showed that cells
were treated with P. amarus extract at 4 0 , 6 0 , and 8 0 µg/ml and 2mM hydrogen peroxide (H2O2)
to significantly (p-value < 0 . 0 5 ) increase cell viability when compared to cells cultured with H2O2 only.
This result relates to the control group, the chemoprotective compound Quercetin, that has been identified
in many plants. Our study indicates that P. amarus extract of 40, 60 and 80 µg/ml can restore cell survival
from oxidative stress induced by Hydrogen Peroxide. This finding suggests that P. amarus can protect
keratinocytes against oxidative stress.
Keywords : Phyllanthus amarus Schumach. & Thonn. , Hydrogen Peroxide , HaCaT cells

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Page 123

Effect of red sticky rice (Oryza sativa L.) bran extract (RRBE) on cell viability of H2O2-treated
Huh7 cells

Banyapon Boonchoo1, Natthanai Sopha1, Nantiya Hongthong1, Narongsuk Munkong2, Atthapan Morchang1
1Deparment of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University
2Deparment of Pathology, Faculty of Medicine, University of Phayao

Oxidative stress is a condition caused by an imbalance between free radicals and antioxidant within
the cell. If the stress prolongedly occurs without proper responses, it will ultimately cause the disease. Most of
the diseases are chronic such as cancer, Alzheimer's disease and coronary heart disease. The treatment
choice for these diseases is to restore the intracellular balance of free radicals and antioxidants. Red sticky rice
(Oryza sativa L.) is a pigmented rice that is widely cultivated and consumed in the Northern, especially in
Phayao province of Thailand. Our unpublished data discovered that red sticky rice bran extract (RRBE)
contained several antioxidants such as phenolic, flavonoid and proanthocyanidins compounds. To investigate
the effect of RRBE on oxidative stress, the toxicity of RRBE on Huh7 cells was firstly test. Treatment of RRBE up
to 1 ug/ml for 24 hours exhibited non-toxicity to the cells. Secondly, hydrogen peroxide (H2O2) was used as the
oxidative stress inducer. Treatment of 10 mM of H2O2 for 5 hours significantly reduced the cell viability to
66.66%. Finally, the effect of RRBE against oxidative stress induced by H2O2was determined in term of 1)cellular
viability, 2)Intracellular reactive oxygen specie (ROS) production and 3)intracellular glutathione production. Our
preliminary data showed that pre-treatment with 1 ug/ml of RRBE restored the cell viability upon receiving H2O2
by 34.33%. Our present data indicates that treatment of RRBE improved the cell survivability upon oxidative
stress insults. The future experiment is to investigate whether RRBE affected ROS and glutathione productions.
Taken together, our study is not only providing the basic scientific fact to support the consumption of red sticky
rice or RRBE as a health-promote food supplement but also increasing the value of the local product.
Keywords: Oxidative stress, Red rice, Red rice bran extract

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Page 124

The Detection of Antibiotics in Pacific white shrimp in the Thonburi region, Bangkok

Patsinee Monwised 1, Wasin Kornpitak 1, Nunthawadee Niamnuy 1, Phrae Saibuadaeng 1,
Ranida Tuanudom 2, Patsarawadee Paojinda 1
1Program of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University
2Preclinical Health Science Center, Faculty of Medicine, Bangkokthonburi University

Thailand is a country of agricultural societies, in which the pacific white shrimp is widely
cultivated. The white shrimp is a significant export from Thailand. During cultivation, various
antibiotics are used and the practice is becoming increasingly more popular.

Antibiotics have become widely used in animals to prevent and treat diseases. The antibiotic
residue in Pacific white shrimp is a serious public health concern due to its potential harmful effects
on consumer health. Problems that may arise are decreasing sensitivity to antibiotics, allergic
reactions and cell mutations.

The purpose of the present research was to detect the presence of antibiotic residues in the
Pacific white shrimp in markets and supermarkets of Thonburi area, Bangkok. The approach taken
was to test samples by using the semi quantitative RR test kit. A total of 35 samples were collected;
which included 30 samples from markets and 5 samples from supermarkets. The tests were divided
into three groups of antibiotics Group A (Tetracycline), Group B (Macrolide, Aminoglycoside and
Sulfonamide) and Group C (Penicillin). This provided 105 tests and detectable quantities of antibiotic
residues was found in the A, B and C groups.

Overall, in the total of 35 samples 34 of the samples (97.1%) showed antibiotic residues.
The residue rate of the three groups combined group A, B and C had the highest rate of 60.0%, then
28.6% for the B and C combination, and finally 11.4% for the presence of antibiotic residues B alone.

It was concluded that there antibiotics were used in all three groups of pacific white shrimp
sampled with Macrolide, Aminoglycoside and Sulfonamide being the main antibiotics being used.
The high rate of detection of antibiotic residues found in this work indicated that the risks associated
with antibiotic residues could not be ignored given the cumulative risk of antibiotics and in particular
the emergence of bacterial resistance to humans.

Keyword: Antibiotics residue, Pacific white shrimp, Markets, Supermarkets, Test kits

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Page 125

The Detection of Antimicrobial agent in Chicken meat in Thonburi region, Bangkok

Aslinda mansan, Jomkuan Khotjantuek, Patsarawadee Paojinda, Nunthawadee Niamnuy
Program of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University

Chicken meat production is among the most rapidly growing industries around the globe, and
chicken is one of the major sources of meat. An increase in demand has put the farmer under continuous
pressure to produce poultry in the shortest period of time with maximum output. The antimicrobial residues in
chicken meat have been determined in many of the studies globally and are considered one of the possible
causes of antimicrobial resistance in human pathogens. This study aimed to detect antimicrobial residues in
chicken meat of the Thonburi area. A total of 35 samples were analyzed using an RR-test kit screening
method. All of the samples contained Tetracycline (Antibiotic group A), Macrolide, Aminoglycoside, and
Sulfonamide (Antibiotic group B), and Penicillin (Antibiotic group C). Tetracycline was the most common
antimicrobial residues detected in 20 samples, which included 6 (75%) of 8 samples from supermarkets and
12 (44.4%) 27 samples from markets), followed by Macrolide, Aminoglycoside, and Sulfonamide which
included 4 (50%) of 8 samples from supermarkets and 16 (59.3%) 27 samples from markets) and a low
number of penicillin residues, about 13 samples, included 4 (50%) of 8 samples from supermarkets and 9
(33.3%) 27 samples from markets). The results have shown that the highest antimicrobial residues rates were
detected for antibiotic group A: tetracycline. We recommended that antibiotic in chicken meals is still a
problem, and that antibiotic residues in poultry meat should be studied more closely.

Keyword: Antimicrobial residues, Chicken meat, RR-test kit

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Page 126

The Latent Fingerprint Analysis by VeriFinger 12.0 Software

Arthittaya Ainwarit1, Panisa Narawutthiphan1, Siraprapha Klamkliang2, Ratchaneewan Aunpad3, Duangnate Pipatsatitpong1
1Department of Medical Technology and 3Graduated program, Faculty of Allied Health Sciences, Thammasat University.
2Saraburi Provincial Police Forensic Science.

Recently, fingerprint verification by experts combined with The Automated Fingerprint Identification
System (AFIS) is the standard method for fingerprint verification. The Police Forensic Science at Saraburi
Province, the collected fingerprints may be incomplete or have less than 10 minutiae points and the experts'
workload increases accordingly due to the increased number of fingerprints to be analyzed. This study aims to
assess the performance of the VeriFinger 12.0 software for detecting latent fingerprints to use in the initial
fingerprint verification before sending it to the experts for inspection by Mini AFIS. The study population
consisted of 6 volunteers. The fingerprint samples were collected from all five fingers by stamping them on two
materials including smooth surface (glass) and rough surface (wood) and then collecting latent fingerprints with
a dusting method that use clear tape. Then, the latent fingerprint pictures were analyzed with VeriFinger 12.0
and Mini AFIS software and calculated for %Correct, %Missed, and %False to compare the passive fingerprint
verification performance of the standard method (Mini AFIS with experts). The results showed that %Correct
was 84.9, %Missed was 15.1, and %False was 49.4. It indicated that VeriFinger 12.0 defined minutiae point
matching the standard method accounting for 84.9%, 15.1% of minutiae points that were not detected
according to the standard method, and 49.4% of minutiae were misconfigured or different from the standard
method. From the results of the latent fingerprint verification performance comparison between VeriFinger 12.0
software and mini AFIS, the %Correct was 85.0, %Missed was 15.0, and %False was 48.7 respectively. The
correlation between VeriFinger 12.0 versus the standard method and Mini AFIS, the Spearman correlation of
%Correct, %Missed, and %False were 0.553 to 0.907, which were statistically significant (p<0.001). In
standard method and Mini AFIS software.
Keywords: VeriFinger 12.0, Mini AFIS, Latent Fingerprints, Forensic Science, Minutiae

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Page 127

Assessing the requirement for the information systems to manage medical laboratory
equipment and material

Parincha Dampan, Chatchada Butsri and Somchat Taertulakarn
Department of Medical technology, Faculty of Allied Health Sciences, Thammasat University

At present, the computer and information systems provide important role in the management system.
In the medical laboratories have used information systems to help in the management of medical devices,
medical equipment and reagents, materials/ consumables for the management information system but there
are still many limitations and not complete according to the needs of users. Therefore, this study aimed to
assess the management of medical laboratory equipment and materials, as well on assess knowledge of
management system and the need for equipment and material management systems for medical laboratories.
Initially, we designed questionnaire about the management of reagents/consumables and medical laboratory
instruments and inquire 100 medical laboratories. Data were calculated based on descriptive statistics. From
the collection of assessments,64 medical laboratories questionnaire was responded. 60 medical laboratories
had a system for managing equipment and materials accounted for 93.8%.53 medical laboratories were used
computer systems in the management systems accounted for 82.8%. And 75% of all point the information
systems in management of medical laboratory equipment and materials are necessary. The results of the study,
it indicates that most of the medical laboratories have a need for information systems to manage the equipment
and materials of medical laboratories.
Keyword: Medical Device Management System, Material/Supply Management System and Information System

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Page 128

Mini Data mining Discriminating Iron overload, Hypochromic microcytic anemia and Normal
individual using RBC Indices in Pra Intaracha populations

Panichar seubsooksakulvong, Sumittra jitsuvantaya, Chollanot kasat and Ajchara phu ang1
Department of medical technology, Faculty of Allied Health Science, Thammasat university
1College of Innovation, Thammasat University

Background Nowadays, Machine learning has wildely used in healthcare systems The previous works
had revealed the relevance of data mining and the improving of computational power in various aspects of
biomedical application Objective To apply three machine learning, including Random forest RF , Decision
tree DT and Neural network NN for discriminate Iron overload, Hypochromic microcytic anemia HMA and
Normal individual Methods A 157 sets of laboratory data were obtained from Pra Intaracha population who
had received health checkup in 2019 The rapid miner, Data science software was performed for data
preparation, machine learning, deep learning, and predictive analytics Results Forward selection was used to
discriminating Iron overload and normal individual, The highest accuracy 72 7 achieved using NN and DT
followed by RF 70 9 NN and RF revealed accuracy of 91 3 in differentiating HMA and normal individual
and followed by DT 89 8 For differentiating Iron overload and HMA, the highest accuracy 89 6 produced
using NN followed by DT and RF 79 2 and 76 6 respectively Backward selection was also performed to
discriminating Iron overload and normal individual, the highest accuracy 74 6 performed by NN followed by
RF and DT 70 9 , 63 64 consecutively RF show the highest accuracy 91 3 for classify HMA and normal
individual followed by NN and DT 88 1 and 84 3 respectively For differentiating Iron overload and HMA,
NN achieved the highest accuracy 87 0 followed by RF and DT 81 8 and 80 5 respectively For
classifying Iron overload, HMA and Normal individual, NN Forward selection performed the higher accuracy
73 9 compared with NN Backward selection 72 6 Conclusion Our study shows that a machine learning
predictive model based on routine hematological parameters can be applied to discriminating Iron overload,
HMA and Normal individual Further study into large population is recommended

Keywords Data mining, Anemia, Iron overload, Hypochromic microcytic anemia

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Page 129

Evaluating the effectiveness of Lac dye for developing Bloody fingerprint

Nisachon Siriphan, Chanaporn Boonsong and Chollanot Kaset
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University

Background Many enhancing agents can be used to develop the latent bloody fingermark for example
Amido black protein stain , ninhydrin amino sensitive and Leuco Crystal Violet Heme stain However, some
enhancing agents are toxic and health hazard Thus, the use of natural products for bloody fingermark
enhancement has several advantages and challenges Lac dye, secretion of Lac scale insect, have been used
to enhance bloody fingermarks with high quality fingermark grading on non porous surfaces Objective To
study the correlation between the quality of bloody fingermark grading and the number of minutiae after Lac
dye or Amido Black staining Methods All data were obtained from Lac dye and Amido black stained bloody
fingermark on various types of surface materials An average grading score evaluated by 10 different
fingermark examiners were used to analyzed with the number of minutiae as analyzed by AFIS Results
Overall, there was a strong correlation between grading score and the number of minutiae Conclusion The
grading score of developed bloody fingermarks can be used as a criterion for prediction of number of minutiae
Moreover, Lac dye could be used as an alternative to chemical dye such as Amido Black on non porous
materials
Keywords Lac dye, Amido Black, Bloody fingermark, Non porous surfaces, Porous surfaces

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Page 130

The development of questionnaire on knowledge and attitudes among Thai adolescents
towards the ban on blood donation from gay and bisexual men

Ismail Bunyamin1, Apirati Inprakhon1 and Anek Pootong1,2
1Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University
2Research Unit in Health, Educational, and Social Equity in Sexual and Gender Diversity, Thammasat University

Background: "Simply being a man who has sex with men is not a good enough reason to exclude someone from
donating blood" they said. Objective: In this study, we aimed to develop the questionnaire on knowledge and
attitudes among Thai adolescents towards the ban on blood donation from gay and bisexual men. Methods:
the questionnaire was designed and content validity was then evaluated by by an LGBTQ and blood bank
expert panel. Questionnaire was tested in pilot study (n=35) and reliability was assessed by using the alpha
coefficient according to Cronbach's method. Results: The questionnaire was composed of 44 item in 3 domains
1) basic information of volunteer 2) knowledge and 3) attitudes toward blood donation and ban on blood
donation by gay and bisexual men. Index of content validity in each three domain of the questionnaire was
obtained over 0.7. The Cronbach’s alpha as reliability of questionnaire was 0.790 for knowledge plus attitudes,
0.274 for knowledge and 0.803 for attitude. Conclusion: The results showed that the questionnaire on attitude
toward blood donation and ban on blood donation by gay and bisexual men was a valid and reliable. However,
the knowledge questions still needs to be revised.
Keywords: ban on donating blood, gay and bisexual men, questionnaire

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Page 131

Development of questionnaire of knowledge, attitude, and willingness in blood donation among
Thai gay and bisexual men

Kokeat Kobkoy1, Supichaya Chanachairoj1, Anek Pootong1,2
1Department of Medical technology, Faculty of Allied Health Science, Thammasat University
2Research Unit in Health, Educational, and Social Equity in Sexual and Gender Diversity, Thammasat University

Blood donation rules for gay and bisexual men (GBM) are being relaxed in several countries. The
previous study showed that a change in deferral policy result in a proportional increase in GBM donating
blood. Thus, the study on the willingness to donate blood among in Thai GMB was interesting. In this study,
we aimed to develop a questionnaire of knowledge, attitude, and willingness in blood donation among gay
and bisexual men in Thailand. This study comprises two phases, instrument design and evaluation of validity
and reliability. A questionnaire consisting of 4 domains (demographic data, knowledge, attitudes, and
willingness to donate blood) was designed by our research team. Content validity was examined by an LGBT
and blood bank expert panel. Reliability was evaluated among Thai gay and bisexual men (n=30). Preliminary
versions consist of 42 questions for 4 domains. It was found that 39 questions had high content validity with
IOC range from 0.50 to 1.00. Unfortunately, the internal consistency Cronbach alpha values on knowledge,
attitude, and willingness were -0.03, 0.41 and -0.03, respectively. In conclusion, this questionnaire was not
suitable for data collection. The further revision is still needed.
Keyword: Bisexual men, Blood donation, questionnaire, Gay men

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Page 132

Age-associated DNA methylation in colorectal cancer: A systematic review

Pipisorn Ganhasing, Sunantisa sinthinanan and Pairoa Praihirunkit.
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University.

It has been evident that DNA methylation is associated with human age. A number of research have
applied DNA methylation for age prediction from a variety of specimens, including blood. In addition, various
diseases, including cancer, can also influence the level of DNA methylation. This research needs to collect
relevant research articles to the use of DNA methylation to estimate age from blood samples and research
papers that analyze DNA methylation in colorectal cancer. The aim of this study was to assess whether
colorectal cancer factors affects DNA methylation for age prediction. This study conducted a systematic review
approach based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 (PRISMA
2020) guidelines. We have searched research articles from the Scopus database published between 2010 and
2021. The research articles were divided into two groups: 1) Research paper on DNA methylation for age
prediction from blood samples and 2) Research paper comparing DNA methylation in colorectal cancer
patients and controls from blood samples. We selected 30 research articles on DNA methylation for age
prediction and 51 research articles on colorectal cancer. The results revealed that top five genes that were
commonly used in age predictions were ELOVL2, KLF14, FHL2, TRIM59 and C1orf132, with mean absolute
deviations (MAD) between 3.29-4.18 years. Research articles on colorectal cancer revealed that top five genes
associated with the disease were SEPT9, C9orf50, SDC2, BCAT1 and VIM. From the analysis of the two
research groups (age prediction and colorectal cancer), it was revealed that the genes used to predict age did
not overlap with the genes associated with colorectal cancer. Therefore, it is possible that colorectal cancer
does not affect DNA methylation for age prediction in the blood.

Keywords: DNA methylation, Age-related DNA methylation marker, Age estimation, colorectal cancer

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Page 133

The Detection of Antimicrobials agent in Pork in the Thonburi region, Bangkok

Waraporn Panchit, Linda Wongphan, Suwanan Nawiang, Patsarawadee Paojinda, Nunthawadee Niamnuy
Program of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University

Pork is a great source of protein and has important health benefits. However, pork may be
contaminated with antimicrobial compounds that farmers often use to optimize their farming
processes. which these drugs can cause harm to consumers when eating for a long time. There are
many residues in the body causing an allergic reaction to the drug or resistance to that drug.

The researcher realized the importance of this problem and therefore was interested in
studying the detection of antimicrobial residues in pork in the Thonburi region, Bangkok. Because it
is an old community where people live as large families, prefer to consume pork from markets or
department stores in the area. A total of 35 samples were collected; which included 28 samples from
fresh markets and 7 samples from supermarkets. The RR Test Kit was used to test the residues of
three drug classes: Group A (tetracycline), Group B (sulfonamide), Group C (penicillin).

Overall, in the total of 35 samples, the results showed that 14 samples (40%) contained
Group A antimicrobials and 10 samples (28.6%) were expected to have Group A drug residues. Six
samples (17.1%) contained Group B antimicrobials and 6 samples (17.1%) were expected to have
Group B drug residues. Six samples (17.1%) contained Group C antimicrobials and 19 samples
(54.3%) were expected to have Group C drug residues. There was no statistically significant
difference between the samples from the fresh market and the supermarket (p-values>0.05).

Residues were found in pork samples at high rates. Indicates the potential for harm to
consumers. Therefore, the government and the Ministry of Public Health should focus on controlling
and preventing the use of antimicrobial agents. This is to ensure long-term safety for consumers.
Keyword: Antimicrobial residue, Fresh market, Supermarket, RR Test Kit

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Page 134

The Study of the diversity of the genetic code and the chemical-physical properties of
peroxidase enzymes.

Napat Pimsiri 1, Waranya Imprasittichai 2, Patsarawadee Paojinda 1
1Department of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University
2Department of Basic Medical Science, Faculty of Medicine Vajira Hospital, Navamindradhiraj University

The peroxidase is widely use enzymes and synthesized by plants in response to the natural
environment. Peroxidases are a group of oxidoreductases which mediate electron transfer from hydrogen
peroxide (H2O2) and organic peroxide to various electron acceptors. Found in variety of species ranging from
microorganisms to animals their main functions differ from organism to organism. They possess a broad
spectrum of impact on industry and fungal biology. There are numerous industrial applications using
peroxidases. For example, catalyse highly reactive pollutants and to breakdown lignin for recycling of carbon
sources. Moreover, genes encoding peroxidases play important roles in fungal pathogenicity in both humans
and plants. All of this study, the genetic diversity of peroxidase enzymes using amino acid sequences in
seven different organisms of plants, bacteria, human and yeast by using bioinformatics tools (Clustal W and
ExPASy SIB Bioinformatics SOPMA tool). The amino acid sequences of peroxidases for seven different
organisms were retrieved from NCBI (http://www.ncbi.nlm.nih.gov/protein), UniProt proteomic server
(http://www.expasy.org/). Organisms were selected according to the difference species (plants, bacteria,
human and yeast). The physiochemical data of amino acid sequences were generated from the ExPASy and
multiple sequence alignment and phylogenetic analysis from the Clustal W program. In this study has clearly
worked out prominent differences between seven different organisms of peroxidases in conserved amino acid
residues at several points, percent identity of peroxidase s amino acid sequences in seven different
organisms was found in the range of 11.11 to 65.70, the number of amino acid sequences obtained from the
NCBI database had different lengths, with a length of 58-352 amino acid residues, and in the phylogenetic
grouping of the seven organisms, Glycine max and Ipomoea batatas were grouped together with the closest
genetic correlation. The results of the research will indeed be of great help to get an insight of the peroxidase
enzymes of seven different organisms, to understand the role of amino acids to develop practical strategies in
engineering these peroxidases and their potential use in different industries, their role in biological and in
bioremediation processes.

Keywords: Peroxidase, Amino acid sequences, bioinformatics, genetic diversity

MS

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Organizing Committee

Faculty of Allied Health Sciences, Chulalongkorn University
Coordinator:
Asst.Prof.Dr. Panan Ratthawongjirakul
Asst.Prof.Dr. Kamonlak Leecharoenkiat
Dr. Siriporn Sangsuthum

Faculty of Allied Health Sciences, Thammasat University
Coordinator:
Asst.Prof.Dr. Chareeporn Akekawatchai
Asst.Prof.Dr. Kanyanath Piumngam

Faculty of Allied Health Sciences, Burapha University
Coordinator:
Asst.Prof.Dr. Pornanan Kueakhai
Asst.Prof.Dr.Kanokporn Srisucharitpanit
Dr.Tistaya Semangoen

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Faculty of Allied Health Sciences, Bansomdejchaopraya Rajabhat University
Coordinator:
Asst.Prof.Dr. Chotechuang Panasophonkul
Dr. Atthapan Morchang
Faculty of Allied Health Sciences, Huachiew Chalermprakiet University
Coordinator:
Lect. Nontawit Pirat
Dr.Tanasan Sirirat

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MT Student Staff, Faculty of Allied Health Sciences, Chulalongkorn University

Kodchamon Suthidee Natcha Chayaburakul Napassorn Satayapat
Kantapat Simmalee Nuttakarn Yaowalak Nantakarn Suayong
Kanjanaphan Santiworanart Natchanon Khanmasom Niparat Seehabut
Kantheera Nutchaworada Nirawan Wasukree
Tangwongsupang Thitirattananon Nusara Pusomta
Kunlanat Yimnoi Nathnaree Taebunpakul Boossapasorn Booyasirisri
Kulsiree Sutthirat Natthaphum Saenkhampha Panithi Ronkhao
Kenika Sukrong Nutsaraporn Yaiyong Prattana Khamthakhruea
Kanitta Intorn Nichagorn Leekhammong Paweena Jatuten
Kunanya Homphan Nichapat Wiangha Panchanit Prikchoo
Jantima Nanongkhai Dolaporn Pattarathitinuruk Panisara Kaenkong
Jaruda Witoonsaridsin Donlawat Wasuanan Pojjananat Sukkhang
Chutchanok Sonkumharn Dararatsamee Pornlada Chaijaroen
Chananchida Kamtan Muangmanee Pattapon Kittivithitkun
Chutikarn Mahayosanun Tavinee Unjaroen Pichayada Sawasdee
Napreechaya Mogmued Naphachon Phuangphaga

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Pimploy Kanitsap Vanida Sukvijit Saralchana Buhsaban
Pimsiri Onsaad Waratchaya Poolnoi Salintip Athiwatthanawong
Penyupha Warangkanang Youle Siriyakorn Chiemchit
Woraditsakulchai Weena Montridilok Sutinan Yokmanee
Pensurad Uipanit Weeraya Panyameesamer Saowapark Yahom
Fasai Jainuan Sirisopha Chandrasakha Akira Wongvichien
Pattaraporn Pintawa Siwakorn Seedapalee Anuchit Dathong
Pawarin Aeknam Supphawet Apichaya Mongkolvorawan
Mintra Supcharoenpaisarn Tangjirawattana Apinya Paipong
Rungfa Boonrang Supapit Areerob Ariya Fujii
Wanaketa Komwaing Supapit Uapattanakul

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