Understanding Your Analyzer 2-26 NOTE More than one check box can be chosen at the same time in one setting option. Text box Click the text box, and then you can start editing when the cursor appears. You can enter the characters from the location of the cursor and the cursor moves to the right at the time. A hospital name is entered into the text box as follows: You can also proceed with the following operations in the text box: Move the cursor to the left or right by using the [←], [→] keys on the keyboard. Move the cursor to the left of the initial character or the right of the end character by pressing the [Home], [End] keys on the keyboard. Delete the character on the right of the cursor by using the[Delete] on the keyboard. Delete the character on the left of the cursor by using the[Backspace] key on the keyboard. Switch to other text box by using the [Tab] key on the keyboard. NOTE Text boxes of different use require different input characters. You don't have to enter the separators in the date box and the IP box. The scroll bar (horizontal/vertical) will appear if the content of the text box can not be displayed in one sight. You can scroll or use the[↑], [↓] keys on the keyboard to view the information fully. Combo box The combo box consists of a text box and an arrow button, which is shown below: See "Arrow button of the combo box" for details about selecting. See "Text box" for details about editing if it is editable.
Understanding Your Analyzer 2-27 Date Text box The date text box is shown below: See "Arrow button of the date text box" for details to complete date selection, or see "Text box" for details to edit date in the date text boxes. Form/Table The form contains several cells and check boxes (sometimes). Click a cell, it is chosen as shown below: Then, you can proceed with the following operations: Select the cell by using the [↑], [↓], [←], [→] keys on the keyboard. Select the initial or end form unit of the current row by using the[Home], [End] keys on the keyboard. For an editable form unit, a cursor will appear in it if it is double clicked. You can enter the characters from the location of the cursor and the cursor moves to the right at the time. See the following figure for the cell under editing status.
Understanding Your Analyzer 2-28 You can proceed with the following operations in the cell: Move the cursor to the left or right in the form unit by using the[←], [→] keys on the keyboard. Move the cursor to the left of the initial character or the right of the end character by pressing the [Home], [End] keys on the keyboard. Delete the character on the right of the cursor by using the [Delete] on the keyboard. Delete the character on the left of the cursor by using the [Backspace] key on the keyboard. Hide the cursor and quit editing by using the [Enter] key on the keyboard. In some forms/tables, you may perform the following operations: Adjust the position of a column Click and hold the title of the column, and then drag the column horizontally to the desired position. Adjust the width of a column Click and hold the boundary line between two columns, then drag the line to adjust the width of the columns.
Understanding Your Analyzer 2-29 Sort Click the title of a column, the rows will be sequenced by the condition of the column in ascending/descending order, click the title again to change to descending/ascending order. Select more than one row If you want to select some contiguous rows: 1. Select the first row. 2. Press and hold “Shift” on the keyboard, and select the last row. All rows between the first and last rows will be selected. If you want to select some discontiguous rows: 1. Select the one row. 2. Press and hold “Ctrl” on the keyboard, and select other row(s). Dialog box There are many types of dialog box with varied functional buttons, which are the "OK" dialog box, the "OK/Cancel" dialog box, the "Yes/No" dialog box, the "Yes/No/Cancel" dialog box, and other dialog boxes of special indication. A dialog box consists of the title area, information area and function button(s). Take the following "Yes/No/Cancel" dialog box for example: After changing the settings, click to close the dialog box and save the change; or click "No" to close the dialog box without saving the change. Click the button on the right of the title area to close the dialog box without performing any operation.
Understanding Your Analyzer 2-30 2.6 Help Click the icon to view the software help information.
Understanding Your Analyzer 2-31 2.7 Reagents, Controls and Calibrators As the analyzer, reagents (diluent, rinse, lyses, probe cleanser and E-Z cleanser), controls, and calibrators are components of a system, performance of the system depends on the combined integrity of all components. You should only use the Mindray-specified reagents (see Appendix B Specifications), which are formulated specifically for the fluidic system of your analyzer in order to provide optimal system performance. Do not use the analyzer with reagents from multiple suppliers. In such use, the analyzer may not meet the performance specified in this manual and may provide unreliable results. All references related to reagents in this manual refer to the reagents specifically formulated for this analyzer. Each reagent package must be examined before use. Product integrity may be compromised in packages that have been damaged. Inspect the package for signs of leakage or moisture. If there is evidence of leakage or improper handling, do not use the reagent. NOTE Store and use the reagents as instructed by instructions for use of the reagents. When you have changed the diluent, lyses or cleansers, run a background to see if the results meet the requirement. Pay attention to the expiration dates and open-container stability days of all the reagents. Be sure not to use expired reagents. After installing a new container of reagent, keep it still for a while before use. 2.7.1 Reagent M-68DS DILUENT The M-68DS DILUENT applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It participates in the measurement of parameters related to RBC, PLT, WBC, RET and NRBC. The M-68DS DILUENT is an electric conducting solution formulated to dilute samples and form sheath fluid in the process of blood cell measurement. It participates in cell differentiation, counting and HGB measurement which are accomplished by using the impedance method, colorimetric method, and SF Cube cell analysis technology (3D analysis using information from scatter of laser light at two angles and fluorescence signals). M-68DR DILUENT The M-68DR DILUENT applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It
Understanding Your Analyzer 2-32 participates in the measurement of RET-related parameters together with M-68FR DYE. The M-68DR DILUENT is formulated to lyse red blood cells in blood cell measurement, facilitate the dyeing effect of reticulocytes by M-68FR DYE. It participates in the measurement of RET-related parameters which is accomplished by using SF Cube cell analysis technology (3D analysis using information from scatter of laser light at two angles and fluorescence signals). M-68FR DYE The M-68FR DYE applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It participates in the measurement of RET-related parameters together with M-68DR DILUENT. The M-68FR DYE is formulated to dye reticulocytes with the assist of M-68DR DILUENT. It participates in the measurement of RET-related parameters which is accomplished by using SF Cube cell analysis technology (3D analysis using information from scatter of laser light at two angles and fluorescence signals). M-68LD LYSE The M-68LD LYSE applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It participates in WBC differentiation in the DIFF channel together with M-68FD DYE. The M-68LD LYSE is formulated to lyse red blood cells and process white blood cells in the blood cell measurement, amplify the differences among WBC sub-populations and facilitate the dyeing effect of white blood cells by M-68FD DYE. It participates in the measurement of WBC-related parameters which is accomplished by using SF Cube cell analysis technology (3D analysis using information from scatter of laser light at two angles and fluorescence signals). M-68FD DYE The M-68FD DYE applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It participates in WBC differentiation in the DIFF channel together with M-68LD LYSE. The M-68FD DYE is formulated to dye the white blood cells with the assist of M-68LD LYSE. It participates in the measurement of WBC-related parameters in the DIFF channel which is accomplished by using SF Cube cell analysis technology (3D analysis using information from scatter of laser light at two angles and fluorescence signals). M-68LN LYSE The M-68LN LYSE applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It participates in the measurement of NRBC-related parameters together with M-68FN DYE. The M-68LN LYSE is formulated to lyse red blood cells in blood cell measurement, facilitate the dying effect of NRBCs by M-68FN DYE It participates in the measurement of NRBC-related parameters which is accomplished by using SF Cube cell analysis technology
Understanding Your Analyzer 2-33 (3D analysis using information from scatter of laser light at two angles and fluorescence signals). M-68FN DYE The M-68FN DYE applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It participates in the measurement of NRBC-related parameters together with M-68LN LYSE. The M-68FN DYE is formulated to dye the NRBCs with the assist of M-68LN LYSE It participates in the measurement of NRBC-related parameters which is accomplished by using SF Cube cell analysis technology (3D analysis using information from scatter of laser light at two angles and fluorescence signals). M-68LB LYSE The M-68LB LYSE applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It participates in WBC counting and measurement of basophil-related parameters. The M-68LB LYSE is formulated for lysing of red blood cells and differential processing of white blood cells in blood cell measurement; it amplifies the differences between the basophils and other WBC sub-populations. It participates in the measurement of basophil-related parameters which is accomplished by using flow cytometry combining with laser scatter method. M-68LH LYSE The M-68LH LYSE applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It is formulated to measure the hemoglobin-related parameters. The M-68LH LYSE is formulated to lyse red blood cells, release hemoglobin in red blood cells and transform it into methemoglobin, which enables the measurement of hemoglobin-related parameters by using the colorimetric method. PROBE CLEANSER The PROBE CLEANSER applies to Mindray BC-6800/BC-6600 Auto Hematology Analyzer. It is used to clean the analyzer regularly. The PROBE CLEANSER is a cleaning solution which has sodium hypochlorite. It can clean the fluidic channels and tubing, and get rid of blood albumin and sediment.
Understanding Your Analyzer 2-34 2.7.2 Controls and Calibrators The controls and calibrators are used to verify accurate operation of and calibrate the analyzer. The controls are commercially prepared whole-blood products used to verify that the analyzer is functioning properly. They are available in low, normal, and high levels. Daily use of all levels verifies the operation of the analyzer and ensures reliable results are obtained. The calibrators are commercially prepared whole-blood products used to calibrate the analyzer. Read and follow the instructions for use to use the controls and calibrators. All references related to controls and calibrators in this manual refer to the controls and calibrators specifically formulated for this analyzer by Mindray. You should buy those controls and calibrators from Mindray or Mindray-authorized distributors.
3-1 3 Understanding the System Principles 3.1 Introduction The principles used by this analyzer for measurement are: sheath flow impedance method, laser scatter and SF Cube cell analysis technology (3D analysis using information from scatter of laser light at two angles and fluorescence signals) for cell differentiation and counting; the colorimetric method for HGB measurement.
Understanding the System Principles 3-2 3.2 WBC Measurement 3.2.1 SF CUBE Cell Analysis Technology SF Cube is a pathbreaking technology for reliable blood cell analysis, including WBC differential, Reticulocytes and NRBC with efficient flagging. After reaction with proprietory reagents, the targeted blood cells undergo 3D analysis using information from scatter of laser light at two angles and fluorescence signals. The 3D scattergram builds the power to better identify and differentiate blood cell populations, especially to reveal abnormal cell population undetected by other techniques. Laser Flow Cytometry In normal peripheral blood, white blood cells can be classified into 5 categories: lymphocytes, monocytes, neutrophils, eosinophils and basophils. Analyzing all types of white blood cells will provide a great deal of useful information for the clinical diagnosis of diseases. Under the influence of certain diseases, the peripheral blood may contain various abnormal cells apart from the five subpopulations of normal cells, such as atypical lymphocytes, immature cells, etc. Most of these abnormal cells are different kinds of immature cells in the cell generation process. But what they have in common is they contain a great deal of nucleic acid (DNA and RNA), the content of which decreases as the cell gets maturer. Therefore, normal cells and immature cells can be differentiated by detecting the content of nucleic acid in the cells. BC-6800 adopts the SF Cube cell analysis technology to recognize and detect the immature cells in blood accurately besides doing WBC 5-part differentiation.
Understanding the System Principles 3-3 SF Cube Technology In the DIFF channel of the auto hematology analyzer (BC-6800), the fluorescent staining technology is adopted after the sample is mixed with DIFF lyse. The RBCs are lysed and the WBC subpopulations are made different in size and complexity by the lyse; the nucleic acid substances in WBCs are marked by the new asymmetric cyanine fluorescent substance. Due to the different content of nucleic acid in different WBC subpopulations, maturity stages or abnormal development status, the volume of fluorescent dye staining the nucleic acid substances can be different; the low-angle light scatter reflects cell size, the high-angle light scatter reflects intracellular granularity, and the intensity of fluorescent signal reflects the degree that the cell is stained. By sensing the difference in signal in three dimensions of the cells processed with lyse, the DIFF channel differentiates the subpopulations of WBCs (lymphocytes, monocytes, neutrophil and eosinophils), as well as identifies and flags abnormal cells like immature granulocytes, abnormal lymphocytes and blast cells. The lymphocytes are smaller in size with the nucleus taking most part of them. Lymphocytes have a high nucleus-to-cytoplasm ratio, but their nucleic acid content is low, therefore they are at a lower position in the direction of fluorescence and side scatter. The monocytes are larger in size, with high nucleus-to-cytoplasm ratio and high nucleic acid content, and less complex in structure, therefore they are at a higher position in the direction of fluorescence, and have stronger side scatter. The neutrophils and basophils are larger in size, and have medium nucleus-to-cytoplasm ratio and low nucleic acid content, therefore they are at a lower position in the direction of fluorescence, but they have stronger side scatter. The characteristics of the eosinophils are similar to those of the neutrophils, but they contain a lot of alkaline grains, so they have very strong side scatter. The blast cells, atypical lymphocytes and immature granulocytes have high nucleic acid content, so they are at a higher position in the direction of fluorescence on the scattergram. Side scatter RNA/DNA content Internal structure of a cell Forward scatter Fluorescence Laser beam Beam splitter
Understanding the System Principles 3-4 3.2.2 Derivation of WBC-Related Parameters Based on the analysis of the BASO channel scattergram and Bas region, the analyzer gets the White Blood Cell count (WBC) and Basophil number (Bas#). The Basophil percentage (Bas%) is then calculated. Based on the analysis of the DIFF channel scattergram and the Lym region, Neu region, Mon region and Eos region, the analyzer gets the percentage of lymphocytes, neutrophils, monocytes and eosinophils (Lym%, Neu%, Mon%, and Eos%). The number of lymphocytes, neutrophils, monocytes and eosinophils (Lym#, Neu#, Mon# and Eos#) are then calculated based on the sub-population percentages together with the White Blood Cell count got from the BASO channel. The cell numbers are all expressed in 109 /L. WBC count WBC Sum of all particlesin BAS channel except those in Ghost region Basophil number Bas# Particlesin Bas region of BAS channel Basophil percentage 100% WBC Bas# Bas% Lymphocyte percentage 100％ Sum of all particlesin Diff channel except those in Ghost region Particlesin Lym region of Diff channel Lym% Neutrophil percentage 100％ Sum of all particlesin Diff channel except those in Ghost region Particlesin Neu region of Diff channel Neu% Monocyte percentage 100％ Sum of all particlesin Diff channel except those in Ghost region Particlesin Mon region of Diff channel Mon% Eosinophil percentage 100％ Sum of all particlesin Diff channel except those in Ghost region Particlesin Eosregion of Diff channel Eos%
Understanding the System Principles 3-5 Lymphocyte number Lym# WBC Lym% Neutrophil number Neu# WBC Neu% Monocyte number Mon# WBC Mon% Eosinophil number Eos# WBC Eos% 3.2.3 Derivation of NRBC Parameters Nucleated Red Blood Cell percentage 100% Sum of all particlesin WBC region of NRBCchannel Particlesin NRBC region of NRBC channel NRBC% Nucleated Red Blood Cell count NRBC # WBC % NRBC
Understanding the System Principles 3-6 3.3 HGB Measurement 3.3.1 Colorimetric Method According to the Lambert-Beer Principle, when a beam of monochromatic light passes through a well-proportioned non-scattering light-absorbing solution, the absorbance A is proportional to the product of the thickness L and the concentration C. The sample in the HGB channel acts as the light absorbing substance after being treated by reagent, therefore the HGB concentration can be measured by measuring the absorbance. 3.3.2 HGB The HGB is calculated per the following equation and expressed in g/L. Sample Photocurrent Blank Photocurrent HGB(g/L) Constant Ln
Understanding the System Principles 3-7 3.4 RBC/PLT Measurement 3.4.1 Sheath Fluid Impedance Method Sheath Fluid Impedance Method A sensor is designed to enable the RBCs and PLTs to pass through the aperture one by one in a queue under the "focusing" effect of fluid, during which process pulses will be generated according to the Coulter Principle. The backend processor amplifies the pulses and compares them with the voltage thresholds of the RBC/PLT channel, and then the number of pulses in the RBC/PLT channel is calculated. That is to say, the pulses collected are sorted per the voltage thresholds of different channels, the number of pulses falling in the range of the RBC/PLT channel is the number of RBC/PLT. The number of cells in each channel defines the volume distribution of cells. The analyzer presents the RBC/PLT histogram, whose x-coordinate represents the cell volume（fL）and y-coordinate represents the number of the cells. Comparing with the common impedance method, the sheath fluid impedance method is featured by higher efficiency, better signal quality, more accurate analysis results and lower consumption of reagents. 3.4.2 Derivation of RBC-Related Parameters RBC RBC is the number of erythrocytes measured directly by counting the erythrocytes passing through the aperture, which is expressed in 1012/L. MCV Based on the RBC histogram, this analyzer calculates the mean corpuscular volume (MCV) and expresses the result in fL. RBC n 10 / L 12
Understanding the System Principles 3-8 HCT, MCH and MCHC This analyzer calculates the HCT (%), MCH (pg) and MCHC (g/L) as follows, where the RBC is expressed in 1012/L, MCV in fL and HGB in g/L. RDW-CV Based on the RBC histogram, this analyzer calculates the RDW-CV (Red Blood Cell Distribution Width – Coefficient of Variation). It is expressed in form of a percentage. RDW-SD RDW-SD (Red Blood Cell Distribution Width – Standard Deviation) is obtained by calculating the standard deviation of RBC distribution, which is expressed in fL. 3.4.3 Derivation of Reticulocyte-Related Parameters Reticulocyte percentage: 100 (Number of Cellsin Mature RBC Region Number of Cellsin RET Region) Number of Cellsin Reticulocyte Region RET % Reticulocyte number: 100 RET % RBC RET # Low Fluorescent Ratio: LFR 100－HFR - MFR Middle Fluorescent Ratio: 100 Number of cellsin reticulocyte region Number of cellsin MFR region MFR 10 RBC MCV HCT RBC HGB MCH 100 HCT HGB MCHC
Understanding the System Principles 3-9 High Fluorescent Ratio: 100 Number of cellsin reticulocyte region Number of cellsin HFR region HFR Immature Reticulocyte Fraction: IRF MFR HFR 3.4.4 Derivation of PLT-Related Parameters PLT PLT is measured directly by counting the platelets passing through the aperture, which is expressed in 109 / L. MPV Based on the PLT histogram, this analyzer calculates the mean platelet volume (MPV, fL). PDW Platelet distribution width (PDW) is the geometric standard deviation (GSD) of the platelet size distribution. Each PDW result is derived from the platelet histogram data and is reported as 10 (GSD). PCT This analyzer calculates the PCT as follows and express it in ％ 10000 PLT MPV PCT Where the PLT is expressed in 109 /L and the MPV in fL. P-LCR Platelet larger cell ratio (P-LCR) is the ratio of the larger platelet (larger than 12fL) count to the total PLT count. The analyzer calculates the P-LCR based on the PLT histogram and expresses the result in %. In the following figure, S2 represents the number of larger platelet cells, and S1+S2 represents the total PLT count. PLT n 10 / L 9
Understanding the System Principles 3-10 P-LCC This analyzer calculates the platelet large cell count (P-LCC) and expresses the result in 109 /L, P-LCC=PLT P-LCR where the PLT is expressed in 109 /L and the P-LCR in %.
Understanding the System Principles 3-11 3.5 Wash After each analysis cycle, all elements of the analyzer that the sample runs through are washed to ensure no residue is left.
4-1 4 Installing Your Analyzer 4.1 Introduction WARNING Installation by personnel not authorized or trained by Mindray may cause personal injury or damage your analyzer. Do not install your analyzer without the presence of -authorized personnel. The installation, test, update, and modification of the software associated with the analyzer shall only be performed by personnel authorized by Mindray. The analyzer is checked and packed with care before it is shipped from the factory. Inspect the carton carefully after arrival. If you see any sign of damage is found, contact Mindray or your local distributor immediately.
Installing Your Analyzer 4-2 4.2 Installation Requirements 4.2.1 Space Requirements While installing the analyzer, make sure there is enough space left for service and maintaining operations, as well as for the analyzer to dissipate heat and for the fluidic tubing to be properly placed without extrusion. Specific requirements shown as follows: proper height to place the analyzer; at least 500 mm to each side of the analyzer; at least 600mm above the analyzer; at least 150 mm to each side of the pneumatic unit; at least 250 mm behind the analyzer and the pneumatic unit; the diluent container must be placed within 1.0m above or below the analyzer, while the lyse containers must be placed on the same level with the analyzer or within 850mm below the analyzer. CAUTION When installing or using the analyzer, make sure at least the two inside supporters of the autoloader are on the supporting table of the analyzer.
Installing Your Analyzer 4-3 4.2.2 Power Requirements WARNING Make sure the analyzer is properly grounded. Before turning on the analyzer, make sure the input voltage meets the requirements. CAUTION Using pinboard may bring electrical interference and the analysis results may be unreliable. Please place the analyzer near the electrical outlet to avoid using pinboard. Please use the original electrical wire shipped with the analyzer. Using other electrical wire may damage the analyzer or cause unreliable analysis results. Voltage Frequency Power Analyzer a.c. 110V/115V ±10% 50/60Hz ±2Hz 500VA a.c. 220V/230V ±10% 50/60Hz ±2Hz 500VA a.c. 110V/115V ±10% 60Hz ±2Hz 600VA 50Hz ±2Hz 450VA Compressor a.c. 220V/230V ±10% 60Hz ±2Hz 300VA
Installing Your Analyzer 4-4 4.2.3 General Environment Working Environment Storage Environment Operation Environment Ambient Temperature 15℃-32℃ -10℃-40℃ 5℃-40℃ Relative Humidity 30%-85% 10%-90% 10%-90% Atmospheric Pressure 70kPa-106kPa 50kPa-106kPa 70kPa-106kPa The environment should be as free as possible from dust, mechanical vibrations, loud noises, pollution and electrical interference. It is advisable to evaluate the electromagnetic environment prior to operation of this analyzer. Do not use this analyzer in close proximity to sources of strong electromagnetic radiation (e.g. unshielded intentional RF sources), as these may interfere with the proper operation. Do not place the analyzer near brush-type motors, flickering fluorescent lights, and electrical contacts that regularly open and close. Do not place the analyzer in direct sunlight or in front of a source of heat or drafts. The environment should be well-ventilated. Do not place the analyzer on a slope. 4.2.4 Fuse Requirement Fuse specification of the pneumatic unit: 250V T3A WARNING Only install fuses of specified specification on the analyzer. 4.2.5 Transportation and Installation WARNING Installation by personnel not authorized or trained by Mindray may cause personal injury or damage your analyzer. Do not install your analyzer without the presence of -authorized personnel.
Installing Your Analyzer 4-5 The installation, test, update, and modification of the software associated with the analyzer shall only be performed by personnel authorized by Mindray. CAUTION After the autoloader is installed, do not lay to much pressure to it or transport the analyzer by holding the autoloader. Installation of unrelated software on the DMU or other uses of the computer (e.g. play games, log on the internet, etc.) may cause system damage and/or data error. Therefore, please make sure the computer is used for the analysis system only. The transportation and installation shall be conducted by Mindray-authorized personnel. Do not transport or install the analyzer without contacting Mindray or your local distributor.
4-6 4.3 Connecting the Analyzer System 4.3.1 Pneumatic unit Connect the pneumatic unit to the analyzer as shown in the following figure. The operator shall make sure they are connected properly. Figure 4-1 Connecting the Pneumatic Unit
Installing Your Analyzer 4-7 4.3.2 Reagents WARNING Be sure to dispose of reagents, waste, samples, consumables, etc. according to government regulations. The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor. CAUTION There are two ways to discharge the waste: discharge to a waste container, or to directly to a waste disposal channel. If the waste is directly discharged to a waste proposal channel, make sure the outlet of the waste piping is placed lower than the waste outlet of the analyzer Connect the reagent containers to the analyzer as shown in the following figure. Be sure to keep all connecting lines isolated from anything electrical.
Installing Your Analyzer 4-8 Figure 4-2 Connecting the reagents
Installing Your Analyzer 4-9 4.3.3 Optional Equipment Connect the optional equipment to the analyzer as shown in the following figure, and make sure they are connected properly. Figure 4-3 Connecting the Optional Equipment
5-1 5 Customizing the Analyzer Software 5.1 Introduction The BC-6800 is a flexible laboratory instrument that can be tailed to your work environment. You can use the “Setup” program to customize the software options as introduced in this chapter. For the security of the settings and data, two access levels are provided to the operator of the analyzer. The administrator access level provides the operator with access to more functions or settings, some of which can be configured to be accessible to operators. This chapter introduces how to customize the settings of the analyzer and the PC, some of which will be performed only occasionally.
Customizing the Analyzer Software 5-2 5.2 Setting up the SPU 5.2.1 Date Setup ("Menu" > "Setup" > "Date") Click the corresponding radio button to select a date format. 5.2.2 Auxiliary Setup ("Menu" > "Setup" > "Auxiliary Setup") Operator access level Predilute Mode Prompt In the predilute mode, when you press the aspirate key at the sample analysis screen to start analysis, a dialog box will pop up to remind you that the current analysis mode is predilute. Tap on this option to enable or disable the function. Pop-up Keyboard Open Tap on the edit area of each screen, the pop-up keyboard will display for you to input information. Tap on this option to enable or disable the function. Blood Sensor Open
Customizing the Analyzer Software 5-3 Tap on this option to turn on or turn off the blood sensor. Waste Sensor Open Tap on this option to turn on or turn off the waste sensor. Enable Rotary Scan During the barcode scanning in the autoloading mode, the analyzer can automatically rotate the tube to locate the barcode. Tap on this option to enable or disable the function. Administrator access level Besides the settings at the operator’s access level, an administrator can also set up the following items: M68-FD, M68-FR, M68-FN Fluoreagent Sensor Open Tap on this option to turn on or turn off the sensor of the fluorescent reagents. Set up the alarm volume Tap the “Alarm Volume” pull-down list to select the volume of alarm sound. Set up default test panel (a combination of analysis items) Tap the “Default Test Panel” pull-down list to select the default test panel. For indications of test panel abbreviations, see 6.7.1 Selecting work mode.
Customizing the Analyzer Software 5-4 5.2.3 Reagent Setup ("Menu" > "Setup" > "Reagent") 1. Tap “Setup”; 2. Enter the barcode by typing or scanning, and the reagent information will automatically display; 3. Tap “Apply”; 4. Check the “Replace” box of the reagent in the “Reagent Setup” screen; 5. Tap the “Replace” button to replace the reagent in fluidics.
Customizing the Analyzer Software 5-5 5.2.4 Maintenance Setup (Administrator) ("Menu" > "Setup" > "Maintenance") Standby Tap the text box in the "Standby" area and enter the waiting time before entering the standby status. Probe cleanser maintenance Tap the first text box in the "Probe Cleanser Maintenance" area to enter the time to start time-based probe cleanser maintenance. Tap the second text box in the "Probe Cleanser Maintenance" area to enter a time in the text box. Then when the operator cancels the time-based maintenance, a reminder dialog box will pop up after the defined minutes. NOTE Please make sure the numbers you enter are valid and in the required format.
Customizing the Analyzer Software 5-6 5.2.5 Autoloader Setup (Administrator) ("Menu" > "Setup" > "Autoloader") Autoloader Stop Condition Tap the check box in the "Autoloader Stop Condition" area to select one or more stop condition of the autoloader: No Reagent Waste Container Full Sample ID Reading Error Tube Rack No. Reading Error Blood/Analysis Mode Inquiry Failed Tap a radio button in the "Autoloader Stop Condition" area to select how to deal with the sample when the inquiry of its blood/analysis mode failed in the process of the autoloading analysis: Use the mode of the previous sample or Skip after X second(s) (X can be configured by the operator) Rule of Sample ID Increment You can set the rule of sample ID increment in autoloading analysis. Tap the radio button to
Customizing the Analyzer Software 5-7 select the rule of sample ID increment when there is tube vacancy in the tube rack: increases or does not increase Display summary after autoloading Tap the check box at bottom to set whether to display the summary after autoloading completes. 5.2.6 Barcode Setup (Administrator) ("Menu" > "Setup" > "Barcode") The analyzer supports 6 barcode types: CODE 128, CODE 39, IT, CODE 93, CODABAR and UPC/EAN/JAN. Set barcode type and number of digits 1. Tap one barcode type used on site to go to the corresponding setup screen. 2. Check the “Apply” box to apply the code type. 3. Select the number of digits used on site in the “Digits” area. 4. Check the “check bit” box if the barcode in use has check bit. 5. For CODE39, check “FULL ASCII” if needed. 6. Set other code types if needed. For the length limits and check bit of different code types, see B.19 Barcode Specifications.
Customizing the Analyzer Software 5-8 NOTE If you are using ITF, CODE39 or CODABAR with check bit, the “Digits” shall be the sum of the length of the barcode and the check bit. E.g. If you are using barcodes with 9 digits, and select “check bit”, you shall select “10” as the number of “Digits”. For barcode types supporting check bit, use check bit in barcode labels if possible to reduce the rate of misreading. The code types and length limits set on the analyzer shall be those used in your laboratory. Do not select the code types that are not used, which may increase the rate of misreading. Barcodes longer than 20 digits will not be read correctly. Tap the check boxes to select one or more code systems and select the number of digits in the “Digits” pull-down lists or select “unlimited” for unlimited number of digits. For some code systems, there are “check bit” (for barcodes with check bit) and/or “FULL ASCII” (for full ASCII barcodes) check boxes. You can select as needed based on the barcodes you are using. NOTE Using “Unlimited” number of digits may lead to higher rate of incorrect scanning. When “Unlimited” number of digits is selected, and the number of digits scanned is more than 20, the barcode will be displayed as “Invalid”. 5.2.7 Communication Setup (Administrator) ("Menu" > "Setup" > "Communication")
Customizing the Analyzer Software 5-9 Tap the text boxes to enter the IP address, subnet mask and default gateway of the analyzer. 5.2.8 Gain Setup (Administrator) ("Menu" > "Setup" > "Gain Setup") You can view the gain values of different analyses in the “Gain Setup” screen at the operator access level, and modify the HGB gain at the administrator access level. When you modify the HGB default gain, the “Blank volt.” will change accordingly. If the entry is invalid, there will be a note of invalid entry when you try to save the change. 5.2.9 Algorithm Setup (Administrator) ("Menu" > "Setup" > "Alg")
Customizing the Analyzer Software 5-10 The algorithm setup screen shows the value to trigger the i-message flags. I-message is a collection of the flags with uncertainty to some extent. Administrator can modify the reference values of the i-message flags which indicating the degree of certainty (the higher the more probable). The “i-message” tab at the DMU (see graph area > i-message of 7.3.1) shows whether the values set here are met.
Customizing the Analyzer Software 5-11 5.3 Setting up the DMU The DMU provide the following setup configuration screens to users: General Setup Parameter Setup (Administrator) Access Setup Data Dictionary Reference Range (Administrator) Parameter Unit (Administrator) Reexam Rules (Administrator) Screen Display Click “Setup” to go to the setup screen. Saving your settings In all the setup screens, if you want to save your changes, click the “Save” button in the button area to save, or when you leave the current setup screen a dialog box may pop up. Click “Yes” to save the changes, close the dialog box, and go to the screen if specified.
Customizing the Analyzer Software 5-12 Click “No” to close the dialog box without saving the changes, and go to the screen if specified. Click “Cancel” to go back to the previous screen without saving the changes. 5.3.1 General Setup ("Setup" > "General Setup") You can click the following tabs to go to the corresponding setup screens and complete the desired configurations. Auxiliary setup (“Setup” > “General Setup” > “Auxiliary”) You can configure the following items from this screen: Sample ID Other Color setup Delta Check Setup
Customizing the Analyzer Software 5-13 Sample ID Set how to enter the next sample ID Click the pull-down list after “Entry of Next Sample ID”, and select one of the two ways to enter the sample ID: Auto Increase Manual Entry If you select “Auto Increase”, you only need to enter an ID for the first sample (you can choose not to enter and use the default one), and the subsequent sample IDs will automatically increase by 1 based on the previous one. If you select “Manual Entry”, you need to enter each sample ID manually. Set prefix for sample IDs You can use this function when you want to identify a certain batch of samples with a prefix. Type a desired prefix in the text box, and it will be applied to all sample IDs after the change is saved. Set the sample ID after startup Select “Reset Sample ID after Startup”, and enter the desired ID in the subsequent text box to define the ID you want to start with each time after startup (default to “1”); or Select “Sample ID increases based on the last ID before shutdown”, and the sample ID after start up will increase by 1 based on the last sample ID analyzed before shutdown. Others Check box area
Customizing the Analyzer Software 5-14 Select one or more check boxes to enable the function(s); deselect to disable. Automatically delete analyzed sample records from worklist Auto Entry of Draw Time Auto Entry of Order Time Suspect flag Enter one character in the text box to define the suspect flag (default to “R”). High/low flag Enter one character in each combo box or select from the pull down list to define the high and low flags (default: “H” for high and “L” for low). Color setting Color of high/low flag You can set color for font and background of high/low flag. Click “Text color” or “Background color” radio button, and then click the corresponding “Setup” button to define the color. You can check the preview in the “Display Preview” box. Color of Printed Results Click the “Setup” button to select the background color of printed results. You can check the preview in the “Display Preview” box. Color of Validated Results Click the “Setup” button to select the background color of validated results. You can check the preview in the “Display Preview” box.
Customizing the Analyzer Software 5-15 Delta Check Setup Delta Check (deviation control of patient results) calculates the deviation of two groups of analysis results of a patient. If the state of a patient is stable, the (delta) value shall be small. △ The report of the value △ s exceeding the predefined limit may help remind the doctor of paying special attention to the patient status. 1. Check the “Start Delta Check” check box to enable the delta check function. 2. Define the maximal number of days between the two results of Delta Check in the “Max Days between two Results” box. 3. Select parameters you want to include in the delta check in the “Criteria” table. 4. If needed, edit the “Outlier Threshold” of the selected parameter(s) which defines the maximum deviation permitted for the delta check. After you enable the delta check function and define the criteria, if the deviation of any selected parameter exceeds the defined limit, this sample will be reported as an outlier of delta check in the “Result Compare” tab of the “Review” screen.
Customizing the Analyzer Software 5-16 Print Setup (“Setup” > “General Setup” > “Print”) You can configure the following items from this screen: Printer Template setup Print setup Report Printer Click the pull-down list to select the default printer. Template setup
Customizing the Analyzer Software 5-17 Choose a report type Click the pull-down list to select the report type from the following options: Report Worklist L-J QC Graph L-J QC Table Parameter QC Graph Monthly QC Graph Trend Graph Result Summary Result List General Summary Summary of Positive Samples Workload Summary Microscopic Exam. Annual Summary Monthly Summary Set the print template There is a table below the “Report Type” combo box showing all the templates available for the selected report type, and the default template is ticked. To change the default template, select the desired template in the table, and then click the “Set to Default Template” button to confirm your selection. You can also click “Template Preview” to check the preview of the template. If you want to customize the template, click “Customize”. For detailed instruction of template customization, see 12 Customizing the Print Template. Print setup Specify copies You can modify the number of copies (default to “1”) by clicking the up and down arrows or re-enter the number in the text box.
Customizing the Analyzer Software 5-18 Adjust margins The numbers in the four boxes on top, bottom, left and right defines the margins to the top, bottom, left and right edges of the page respectively. Click the up and down arrows to modify the margins or enter the numbers in the text boxes. Report Define report title Modify the default report title or enter the desired one in the “Title” text box. Enable/Disable Autoprint Click the “On” radio button to enable autoprint once sample results get to the DMU; or click “Off” to disable autoprint. NOTE You cannot enable the autoprint if the “Print after Validation” check box is selected. Check box area You can click the check boxes of the following options to enable the corresponding settings: Half Page Report (A5)
Customizing the Analyzer Software 5-19 Print Flags Print Reference Ranges Print Result Edited Flags Microscopic Report Print Prompt Autoprint after validation Print Suspect Flags Print Out-of-Range Flags Print after Validation Print Analyzer Temperature Abnormal Flags NOTE The “Autoprint after validation” check box can only be selected when the “Autoprint” function is “On”. Communication Setup (“Setup” > “General Setup” > “Communication”) You can configure the following items from this screen: Communicate through Network Setup Serial Port Setup Protocol Setup Transmission Mode Communicate through