Calibrating Your Analyzer 9-6 9.3.2 Manual Calibration ("Menu" > "Calibration" > "Manual") NOTE If you log in at the operator access level, you can only view the calibration factors. To perform calibration, please log out and then log in at the administrator access level. Do as follows to calibrate the analyzer: 1. At the "Manual” calibration screen, check the calibration factors and calculate the new factors per the following equation. Mean Current calibration factor Reference value New calibration factor ＝ For example: Suppose the WBC reference value of a calibrator is 8.4, and the current calibration factor of the whole blood mode is 98.9％. Run the calibrator under the whole blood mode for 10 consecutive times and take the WBC results of the 10 runs to calculate: 8.1, 8.0, 8.1, 8.1, 8.3, 8.3, 8.2, 8.0, 8.1, 8.3. The obtained CV is 1.5% and Mean is 8.16, which meet the requirements. The new calibration factor is obtained: 101.81% 8.16 98.90% 8.4 New calibration factor＝ ＝ The calculated calibration factors shall be between 75.00％ - 125.00％.In case of an invalid calibration factor, try to find out the reason (e.g. calibration material not thoroughly mixed, misoperation, etc.).Then recalibrate the analyzer and recalculate the calibration factors.
Calibrating Your Analyzer 9-7 NOTE The entered calibration factors shall be between 75.00％～125.00％ (accurate to the second decimal place). 2. Enter the new calibration factors into the factor cell of the parameter that requires calibration. 3. When you switch screen after entering the new calibration factor, a prompt will display. If the entered calibration factors are valid, a dialog box will pop up when you are exiting the screen. Tap “Yes” to save the new calibration factors and the calibration date of the corresponding parameter changes to current system date and is recorded in the calibration history; then, close the dialog box and switch to another screen. If the entered calibration factors are invalid, a dialog box will pop up when you are switching to another screen. Tap "Yes" to close the dialog box and switch to another screen without saving the changes; the original calibration factors and dates will remain the same.
Calibrating Your Analyzer 9-8 9.3.3 Calibration with Calibrator (Administrator) ("Menu" > "Calibration" > "Calibrator") All the samples, controls, calibrators, reagents, wastes and areas contacted by them are potentially biohazardous. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them and the contacted areas in the laboratory. NOTE The calibrator calibration can only be performed under whole blood mode. Only Mindray-specified calibrators shall be used. Mindray will not be responsible for any erroneous result caused by using other calibrators. Do as follows to calibrate the analyzer with calibrators. 1. Enter the lot No. of the calibrator into the "Lot No." box. NOTE See the instruction for use of the calibrators for the lot No., expiration
Calibrating Your Analyzer 9-9 date and the target. The lot No. must be entered. 2. Enter the “Expiration date”. NOTE The expiration date defaults to the current system date. The expiration date shall not be earlier than the current system date. 3. Enter the targets into the "Target" cells. NOTE The entered expiration date should be either the expiration date printed on the labeling or the open-container expiration date, whichever is earlier. The open-container expiration date is calculated as follows: the date that container is opened + the open-container stability days. 4. Prepare the calibrator as instructed by instructions for use of the calibrators. 5. Press the aspirate key to start calibration, the message box will close automatically, and then a progress bar will pop up. 6. After the analysis, the calibration progress bar will close automatically and the analyzer will have different responses to different analysis results. When the current running is done, if there is a parameter whose calibration data is out of its linearity range but still within the display range, then the calibration data will be displayed in the list and a message box will also pop up. Tap “OK” to close the message box, and the data will be deleted from the table without saving automatically. When the running is done, if there is a parameter whose calibration data is out of the display rage, then the non-numeric parameter values "***" will be displayed in the list and a message box will pop up.
Calibrating Your Analyzer 9-10 Tap “OK” to close the message box, and the data will be deleted from the table without saving automatically. The valid results within the linear range will be displayed directly. NOTE Valid calibration results will be highlighted per the default setting, and will be taken to calculate calibration factors. 7. If the calibration factors have not been calculated but you switch to another screen, then a message box will pop up. Tap "Yes" to switch to another screen while discarding the calibration data and closing the message box. The original calibration factors remain. 8. When calibration count has been performed to a sample for n times (n≥5), the analyzer will calculate the Mean, CV% and calibration factors of all the highlighted calibration data automatically. You can select several data to calculate the calibration factors, but only after at least 5 groups of the data are selected can you get the calibration factors. NOTE The out-of-range CV% does not influence the display of calibration factors. When the amount of valid calibration data in the list reaches 10, a message box "Calibration done!" will pop up. Then, if you press the
Calibrating Your Analyzer 9-11 aspirate key again, the analyzer will beep without starting analysis. 9. There may be two cases when you are switching to another screen: If the calibration factors of any parameter is out of the range [75%-125%] or the CV% of any parameter exceeds the reproducibility standard, then the calculated calibration factors of all parameters will not be saved and a message box will also pop up. Tap "Yes" to close the dialog box and switch to another screen. The calibration factors and date of all parameters will not be changed. If the calculated calibration factors of all parameter are within the range [75%-125%] and the CV% of all parameter are also within the reproducibility standard, then a message box will pop up. Tap "Yes" to save the new calibration factors while closing the message box and switching to another screen.
Calibrating Your Analyzer 9-12 9.3.4 Calibration with Fresh Blood (Administrator) ("Menu" > "Calibration" > "Fresh blood") Do as follows to calibrate the analyzer with fresh blood samples. 1. Prepare 3 to 5 normal fresh blood samples as instructed by 6.6 Preparing Samples. 2. Run each of the prepared samples on the reference instrument (or by the reference method) three times at least. Calculate the mean values and use them as the targets. 3. Select the ID of current sample from the box "Sample 1- Sample 5" on the left of the screen. 4. Enter the targets into the "Target" text boxes. 5. Prepare fresh blood sample. 6. Press the aspirate key to start the calibration and the dialog box will close automatically, then a progress bar will pop up. 7. After the analysis, the calibration progress bar will close automatically and the analyzer will have different responses to different analysis results. If the results are out of the linear range but still within the display range, a dialog box will pop up when the results are displayed in the table.
Calibrating Your Analyzer 9-13 Tap "OK" to close the dialog box and the results will be deleted from the table without saving automatically. If the results are out of the display rage, the non-numeric parameter values "***" are obtained and a dialog box will pop up. Tap "OK" to close the dialog box and the results will be deleted from the table without saving automatically. The valid results within the linear range will be displayed directly. NOTE Valid calibration results will be highlighted per the default setting, and will be taken to calculate calibration factors. 8. When calibration count has been performed to a sample for n times (n≥5), the analyzer will calculate the Mean, CV% and calibration factors of all the highlighted calibration data automatically. You can select several data to calculate the calibration factors, but only after at least 5 groups of the data are selected can you get the calibration factors. NOTE The out-of-range CV% does not influence the display of the calibration factors. When the amount of valid calibration data in the table reaches 10, a
Calibrating Your Analyzer 9-14 dialog box of "Fresh blood calibration done!" will pop up; if you press the aspirate key again, the analyzer will beep without starting analysis. 9. Select other calibration sample ID, analyze other samples according to Step 6-8 above to obtain the calibration factors of all samples. NOTE If some parameters have been selected to be calibrated, the column of the parameters will still be displayed in gray when you switch to another blood sample. 10. There may be several cases when switching to another blood sample: If the calibration factors of the blood sample are invalid or the CV% of any parameter exceeds the reproducibility standard, a dialog box will pop up when switching to another blood sample. Tap "Yes" to empty the entered target of the current sample, all the calibration data obtained and each calculated value including calibration factors, then close the dialog box and switch to another blood sample. If the calibration factors have not been calculated, a dialog box will pop up. Tap “Yes” to empty the entered target of the current sample and all the calibration data obtained, then close the dialog box and switch to another blood sample. If the calibration factors of the sample are valid and the CV% of all the parameters do not exceed the reproducibility standard, you can switch to another blood sample
Calibrating Your Analyzer 9-15 directly. 11. After calibration factors of at least 3 fresh blood samples are obtained, tap the "Calculate" button to enter the screen of calibration calculation. 12. Select or deselect the calibration factors of a blood sample for the calculation of the Mean calibration factors by tapping the corresponding calibration factors. The selected calibration factors will be highlighted, and when 3 or more groups of factors are selected, CV% will be re-calculated automatically base on the selected calibration factors. NOTE The out-of-range CV% does not influence the display of calibration factors. When 3 or more groups of calibration factors are selected, the mean calibration factor will be re-calculated automatically base on the selected calibration factors. The mean calibration factors are regarded as invalid if the deviation of absolute value between the calibration factors included in calculating the mean and the original calibration factors reaches or exceeds 5%; a dialog box will pop up when you exit the current fresh blood calibration screen.
Calibrating Your Analyzer 9-16 Tap "Yes" to close the dialog box and exit with the current calibration data emptied, and then switch to another screen. Tap "No" to return to the current screen. Invalid mean calibration factors are displayed in red and followed with a "?". 13. If the mean calibration factors have not been calculated, when you exit the fresh blood screen or switch to another calibration mode, a dialog box will pop up. Tap "Yes" to discard the calibration data, close the dialog box, and switch to another screen or calibration mode. The original calibration factors and date remain the same. 14. If the calculated mean calibration factors are valid, when exiting the fresh blood calibration screen or switching to another calibration mode, a dialog box will pop up. Tap "Yes" to save the current mean calibration factors and refresh the calibration factors and date in the table at the "Calibration Factors" screen. Then, you can switch to another screen or calibration mode. Tap "No" to close the dialog box and switch to another screen or calibration mode without saving the mean calibration factors and all the calibration data.
Calibrating Your Analyzer 9-17 9.3.5 Verifying calibration factors It is recommended that you take the following steps to verify the calibration factors: 1. Run the calibrator at least three times and check whether the means of the obtained results are within the expected ranges. 2. Run the low, normal and high level controls, each for three times at least, and check whether the means of the obtained results are within the expected ranges. 3. Run at least three fresh blood samples with known reference values, each for six times at least, and check whether the means of the obtained results are within the expected ranges.
Calibrating Your Analyzer 9-18 9.4 Calibration History ("Menu" > "Calibration" > "History") The date, operator of calibration, calibration method and calibration mode of the latest 100 calibrations will be displayed in the table on the screen with the latest on the top (No. 1). If more than 100 calibrations were performed, the earliest result will be overwritten. You can click the arrow buttons to view the entire table. You are not allowed to modify or delete contents in the table.
Calibrating Your Analyzer 9-19 9.5 Touch Screen Calibration ("Menu" > "Calibration" > "Touch Screen") CAUTION Do not use anything sharp on the touch screen or strike on it. NOTE Do not click with the mouse to calibrate the touch screen. 1. Tap the “Touch Screen Cal.” button in the middle of the screen. 2. Tap the red dot at the upper left corner of the screen as instructed by the screen display to start the calibration. 3. Then tap the red dot at the lower right corner of the screen. 4. Tap the blue rectangular area in the middle of the screen, and the calibration will be finished and the screen will resume after 1 second.
10-1 10 Servicing Your Analyzer 10.1 Introduction Preventive and corrective maintenance procedures are required to keep the analyzer in a good operating condition. This analyzer provides multiple maintenance functions for this purpose. This chapter introduces how to use the provided functions to maintain and troubleshoot your analyzer. All the analyzer components and surfaces are potentially infectious, take proper protective measures for operation or maintenance. CAUTION Performing unauthorized maintenance procedures can damage your analyzer. Do not perform any maintenance procedures that are not described in this chapter. In case of problems not specified in this manual, contact Mindray Customer Service Department or your local distributor for assistance. Only Mindray-supplied parts can be used for maintenance. For any questions, contact Mindray Customer Service Department or your local distributor. Exercise caution to avoid contact with the sharp sample probe when performing maintenance.
Servicing Your Analyzer 10-2 10.2 Reagent Management All the samples, controls, calibrators, reagents, wastes and areas contacted by them are potentially biohazardous. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them and the contacted areas in the laboratory. WARNING Be sure to dispose of reagents, waste, samples, consumables, etc. according to government regulations. The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor. CAUTION Do not mix the new container of reagent with the residue in the replaced container to ensure accurate measurement. NOTE Please keep the diluent container from severe shock or crashing against other object. Otherwise, the alarming would be unreliable. You need to replace reagents promptly when it is low, depleted, expired, or required to remove an error. Do as follows: Replace with a new container of desired reagent (see 10.8.2 for details); Finish reagent setup on the SPU. (see 5.2.3 for details) You can also check the reagent information (like expiration date, residual volume, etc.) of the reagents at the reagent setup screen of the SPU.
Servicing Your Analyzer 10-3 10.3 Maintaining Your Analyzer Using the Service Program 10.3.1 When and Why Procedure When to Clean Why to Clean Reagent Replacing After replacing a new container of reagent Replace the old reagent in fluidics Reagent Priming the reagent is contaminated WBC/RBC bubbles are reported Replace the old reagent in fluidics; Flow Cell Bubble Removal When abnormal distribution is found in scattergrams To remove bubbles from the flow cell Fluidics Cleaning When all the blank count results exceed their limits To clean the fluidic system Probe Cleanser Maintenance (of fluidic system, flow cell, SRV, and/or aperture) As needed To clean and soak specified components Aperture Unclogging When aperture clogging is reported Unclog the aperture Pack-up When the analyzer is to be left idle for a long time (more than 10 days) Drain the residual reagent from fluidics 10.3.2 Replacing Reagent ("Menu" > "Service" > "Reagent" > "Replace Reagent") The operator can use this program to replace the reagent in fluidics after replacing a new container of reagent or as needed.
Servicing Your Analyzer 10-4 WARNING Be sure to dispose of reagents, waste, samples, consumables, etc. according to government regulations. The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor. You can replace the following reagents in the fluidics at this screen: M-68DS DILUENT M-68LH LYSE M-68LB LYSE M-68LN LYSE M-68LD LYSE M-68DR DILUENT M-68FN DYE M-68FD DYE M-68FR DYE
Servicing Your Analyzer 10-5 NOTE Please keep the diluent container from severe shock or crashing against other object. Otherwise, the alarming would be unreliable. Do as follows to replace the reagent(s): 1. Tap the reagent you want to replace to start replacing. 2. A prompt will pop up when the replacing is finished. 3. Tap “OK” to close the dialog box. 4. Perform the procedures above to replace other reagents if necessary. 10.3.3 Reagent Priming ("Menu" > "Service" > "Reagent" > "Reagent priming")
Servicing Your Analyzer 10-6 WARNING The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor. You can prime with the following reagents at this screen: M-68DS DILUENT M-68LH LYSE M-68LB LYSE M-68LN LYSE M-68LD LYSE M-68DR DILUENT M-68FN DYE M-68FD DYE M-68FR DYE NOTE Please keep the diluent container from severe shock or crashing against other object. Otherwise, the alarming would be unreliable. Do as follows to prime the reagent(s): 1. Tap the reagent you want to prime to start priming. 2. A prompt will pop up when the priming is finished. 3. Tap “OK” to close the dialog box. 4. Perform the procedures above to prime other reagents if necessary.
Servicing Your Analyzer 10-7 10.3.4 Cleaning ("Menu" > "Service" > "Cleaning") Do as follows to clean: 1. Tap the button of the component you want to clean. 2. A prompt will pop up when the cleaning is completed. 3. Tap “OK” to close the dialog box. 4. Perform the procedures above to clean other components if necessary.
Servicing Your Analyzer 10-8 10.3.5 Maintenance ("Menu" > "Service" > "Maintenance") Maintenance with Probe Cleanser You can use the probe cleanser to clean and soak the following components: Fluidic system Flow cell Sample rotary valve (SRV) Aperture Do as follows to maintain: 1. At the "Maintenance" screen, tap the icon of the component you want to maintain. 2. Tap "Yes" and operate as prompted by the dialog box until the count down box of maintenance appears.
Servicing Your Analyzer 10-9 3. When the maintenance is finished, the dialog box "Maintaining finished!" will pop up. Tap “OK” to close the dialog box. Maintaining Apertures Unclogging Do as follows to unclog: 1. Tap the "Unclog" button at the "Maintenance" screen. 2. A prompt will pop up when the unclogging is finished. 3. Tap “OK” to close the dialog box.
Servicing Your Analyzer 10-10 10.3.6 Servicing the Fluidics ("Menu" > "Service" > "Fluidics") Pack-up Do as follows to pack up: 1. Tap “Pack-up”. 2. Tap "Yes" to perform the pack-up procedure as instructed by the pop-up screens. 3. When the pack-up is finished, shut down the analyzer as prompted.
Servicing Your Analyzer 10-11 10.4 Checking the Analyzer Status Using the Status Program The Status Screen displays the current status of the analyzer. You can check the status including: Statistics Temperature&Pressure Version Info. You cannot modify but only browse the status information at the Status Screen. To check the status information at the Status Screen is significant for you to locate and remove errors of the analyzer. 10.4.1 Statistics ("Menu" > "Status" > "Statistics") You can check the above statistic information and for some items you can check the details. Checking the details Tap the "Detail..." button after "Runs", "QC Runs" or "Calibration Runs" to check the detailed information.
Servicing Your Analyzer 10-12 10.4.2 Temp.&Pressure ("Menu" > "Status" > "Temp.&Pressure") You can check the following temperature information of the analyzer: Reaction Bath Flow cell Sheath fluid bath Optical system Preheating bath Diluent Analyzer You can check the following pressure information of the analyzer: Pneumatic unit pressure (250) Pneumatic unit vacuum (-70) Optical channel pressure (160) Sheath flow impedance pressure (40) Diluent cistern pressure (70) WC 1 pressure (-40) The screen displays the current value of each item and the corresponding normal range, which is significant for you to judge and locate errors of the analyzer.
Servicing Your Analyzer 10-13 10.4.3 Version Info. ("Menu" > "Status" > "Version Info.") You can check the current version information of the analyzer software and hardware.
Servicing Your Analyzer 10-14 10.5 Viewing History Logs The log records the key operations performed on the analyzer. It provides the operators an access to review the operating history and service personnel the facilitation of troubleshooting. The DMU can save logs of the recent two years. If number of logs exceeds the upper limit, the latest log will overwrite the oldest one. You can browse and print logs, but cannot delete them. Click “Log” to enter the following screen. 10.5.1 Viewing Logs Defining the date range Enter or select the starting and ending dates to define a desired date range of logs. Selecting the source of logs Select the desired source of logs from the pull-down list, where you can select all logs, logs on the PC workstation, or logs about the SPUs which used to connect to the DMU. Selecting the log type Select the desired log type from the buttons on the left:
Servicing Your Analyzer 10-15 All Logs Setup Adjustment (administrator) Error Info. (administrator) Other Logs 10.5.2 Exporting Logs (Administrator) 1. Click the "Export" button, and a dialog box will pop up. 2. Select one of the following: Select Date Range All Records Selected Records If you select the first radio button (to export the records of selected date range, you should specify the starting and ending date. 3. Click the “Browse” button to specify the directory to save the exported logs. 4. Click “OK” to start exporting.
Servicing Your Analyzer 10-16 10.6 Adjusting Pressure and Vacuum 10.6.1 Introduction Adjusting pressure and vacuum of the pneumatic unit is important in daily use of the analyzer. Once a pressure or vacuum error is reported, do as instructed by the troubleshooting information to adjust pressure or vacuum. Pressure/Vacuum Function 0.25MPa drives the cylinders and pinch valves of the analyzer. 0.16MPa drives the sheath fluid of optical system 0.07MPa drives the waste emptying process of the analyzer and reagent dispensing from volumetric pump 0.04MPa drives the sheath fluid of the impedance measurement module -0.04MPa drives the waste emptying process of baths The location of the pressure and vacuum regulators: 0.25Mpa
Servicing Your Analyzer 10-17 10.6.2 Adjusting Pressure and Vacuum You need to go to the “Temperature&Pressure” screen before adjusting the pressure or vacuum, Tap "Menu" > "Status" > "Temperature&Pressure" 0.16Mpa 0.07Mpa 0.04Mpa -0.04Mpa
Servicing Your Analyzer 10-18 Adjusting the 0.25MPa pressure 1. Loosen the fixing screw of 0.25MPa pressure regulator with a cross-headed screwdriver. 2. Check the pressure value in the “PS1 (250)” column at the “Temperature&Pressure” screen, and then turn the adjustment knob (clockwise to increase the pressure, counterclockwise to decrease) until the value fall in the expected range ((250±10)Kpa). NOTE If the pressure is too high while adjusting, adjust it to the lowest level before readjusting it to the desired level. 3. Tighten the lock screw of the 0.25MPa pressure regulator after the adjustment is finished. Adjusting the 0.16MPa pressure 1. Pull the 0.16MPa pressure regulator outwards. Fixing screw
Servicing Your Analyzer 10-19 2. Check the pressure value in the “PS2 (160)” column at the “Temperature&Pressure” screen, and then turn the adjustment knob (clockwise to increase the pressure, counterclockwise to decrease) until the value fall in the expected range ((160±1)Kpa). 3. Push the regulator inwards after the adjustment is finished. NOTE If the regulator can not be pushed back, turn it a little bit and then push. Adjusting the 0.07MPa pressure 1. Pull the 0.07MPa pressure regulator outwards. 2. Check the pressure value in the “PS3 (70)” column at the “Temperature&Pressure” screen, and then turn the adjustment knob (clockwise to increase the pressure, counterclockwise to decrease) until the value fall in the expected range ((70±1)Kpa).
Servicing Your Analyzer 10-20 3. Push the regulator inwards after the adjustment is finished. NOTE If the regulator can not be pushed back, turn it a little bit and then push. Adjusting the 0.04MPa pressure 1. Pull the 0.04MPa pressure regulator outwards. 2. Check the pressure value in the “PS4 (40)” column at the “Temperature&Pressure” screen, and then turn the adjustment knob (clockwise to increase the pressure, counterclockwise to decrease) until the value fall in the expected range ((40±1)Kpa). 3. Push the regulator inwards after the adjustment is finished. NOTE If the regulator can not be pushed back, turn it a little bit and then push. Adjusting the -0.04MPa vacuum 1. Turn the locknut of the relief valve counterclockwise to loosen it.
Servicing Your Analyzer 10-21 2. Check the pressure value in the “PS5 (-40)” column at the “Temperature&Pressure” screen, and then turn the adjustment knob (clockwise to increase the vacuum, counterclockwise to decrease) until the value fall in the expected range ((-40±1)Kpa). 3. Secure the locknut by turning it clockwise after the adjustment is finished. Locknut
Servicing Your Analyzer 10-22 10.7 Manual Cleaning 10.7.1 When and Why Procedure When to Clean Why to Clean Cleaning the waste collecting trays As needed To remove the buildup in the trays Cleaning the SRV Every 2 months (of operation) To remove blood residue or other buildup in the SRV Cleaning the probe wipe of the open-vial module As needed To remove blood residue or other buildup in the probe wipe Sterilizing the cover of the analyzer As needed To remove the contaminants on the coverr of the analyzer 10.7.2 Opening and Closing the Front Cover You may need to open and close the front cover in some manual cleaning procedures. Be sure to perform strictly according the standard procedures stated below. Opening the front cover 1. Hold the sides of the front cover and lift it upwards. 2. Fix the opened front cover with the stop bar.
Servicing Your Analyzer 10-23 Closing the front cover 1. Hold the front cover steadily, and then release the stop bar. 2. Move the front cover downwards and close it. CAUTION To avoid damage to the components of the analyzer, close the front cover gently. 10.7.3 Cleaning the Waste Collecting Trays Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. Stop Bar
Servicing Your Analyzer 10-24 WARNING Be sure to dispose of reagents, waste, samples, consumables, etc. according to government regulations. The waste collecting trays may contain biohazardous materials. Exercise caution to avoid direct contact with the trays. CAUTION After the analyzer is turned off, wait at least 30 seconds to release the pressure and vacuum accumulated in the pneumatic lines. Do not perform any maintenance or replacing procedure immediately after you turn off the analyzer. Cleaning the waste collecting tray of the open-vial module 1. Open the front cover. WARNING To avoid personal injury, after you lift the front cover, be sure to fix it with the stop bar properly. 2. Pull out the waste collecting tray under the open-vial sample probe.
Servicing Your Analyzer 10-25 3. Wash the tray with clean water. 4. Wipe up the water left in the tray. 5 Install the tray back properly. 6. Close the front cover. WARNING To avoid personal injury, when you close the front cover, be sure to hold it steadily before releasing the stop bar. CAUTION To avoid damage to the components of the analyzer, close the front cover gently.
Servicing Your Analyzer 10-26 Cleaning the SRV waste collecting tray CAUTION When removing the SRV waste collecting tray, do not make the screw thread of the connecting part on top of the sample probe loose, which may bring air in the sample probe and lead to unreliable results. NOTE Reassemble the SRV waste collecting tray by facing the side with the groove up. 1. Shut down the analyzer, and wait several minutes after power-off. 2. Open the front cover. WARNING To avoid personal injury, after you lift the front cover, be sure to fix it with the stop bar properly. 3. Pull out the waste collecting tray under the SRV.
Servicing Your Analyzer 10-27 4. Wash the tray with clean water. 5. Wipe up the water left in the tray. 6. Install the tray back properly.
Servicing Your Analyzer 10-28 7. Close the front cover. WARNING To avoid personal injury, when you close the front cover, be sure to hold it steadily before releasing the stop bar. CAUTION To avoid damage to the components of the analyzer, close the front cover gently. Cleaning the waste collecting tray of the autoloading module 1. Shut down the analyzer, and wait several minutes after power-off.
Servicing Your Analyzer 10-29 2. Open the front cover. WARNING To avoid personal injury, after you lift the front cover, be sure to fix it with the stop bar properly. 3. Pull out the waste collecting tray under the autoloading piercing unit. 4. Wash the tray with clean water. 5. Wipe up the water left in the tray. 6. Install the tray back properly. 7. Close the front cover. WARNING To avoid personal injury, when you close the front cover, be sure to hold it steadily before releasing the stop bar. CAUTION To avoid damage to the components of the analyzer, close the front cover gently.
Servicing Your Analyzer 10-30 10.7.4 Cleaning the SRV Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. WARNING Be sure to dispose of reagents, waste, samples, consumables, etc. according to government regulations. The sample probe and the SRV may contain biohazardous materials. Exercise caution to avoid direct contact with the probe and the SRV when working around them. If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor. CAUTION After the analyzer is turned off, wait at least 30 seconds to release the pressure and vacuum accumulated in the pneumatic lines. Do not perform any maintenance or replacing procedure immediately after you turn off the analyzer. The SRV is so fragile that any knock or drop may damage it. Exercise caution when disassembling/assembling or cleaning the SRV. Do not loosen or bend the tiny steel tubes at sides of the SRV. Do not loosen or deform the sample probe when disassembling, cleaning and reassembling the SRV. 1. Shut down the analyzer, and wait several minutes after power-off.
Servicing Your Analyzer 10-31 2. Open the front cover. WARNING To avoid personal injury, after you lift the front cover, be sure to fix it with the stop bar properly. 3. Remove the SRV waste collecting tray (see 10.7.3) and pull the probe wipe assembly down to the lowest position.
Servicing Your Analyzer 10-32 4. Remove the probe wipe from the sample probe. CAUTION The sample probe should be completely separated from the probe wipe when the SRV is disassembled. Otherwise, the probe may be deformed or the probe wipe may be damaged. 5. Remove the pressure screw by turning it counterclockwise.
Servicing Your Analyzer 10-33 6. Remove the sampling valve (the outer piece) and the rotor valve (the middle piece) of the SRV respectively. CAUTION When the SRV is disassembled, reagent may leak from the tubes.
Servicing Your Analyzer 10-34 Place a dry cloth or tissue under the SRV to absorb any reagent. Do not remove the rear fixed valve of the SRV. Do not use too much force to pull tubes on sides of the SRV when disassembling the SRV. Otherwise, the tubes may be disconnected and the leakage may be caused. 7. Inject some probe cleanser into holes and grooves of the sampling valve, rotor valve and rear fixed valve respectively. You can also clean the holes and grooves gently with a brush dampened with probe cleanser. 8. Use clean lint-free tissues dampened with probe cleanser to wipe the contact surfaces of the valves. Then wash the valves with distilled water. CAUTION Make sure no dust should be found in the holes and grooves and on the contact surfaces after the cleaning. Unclean surface(s) may lead to leakage from the SRV and unreliable analysis results.
Servicing Your Analyzer 10-35 9. Install the rotor valve and sampling valve back properly. CAUTION While installing the rotor valve of the SRV, the plane part shall be placed horizontally on the top, and the metal knob shall be placed between the two stoppers. Otherwise, the SRV will not be able to work properly. NOTE The valve contact surfaces must be damp when the SRV is reassembled. Be sure the sampling valve, rotor valve and rear fixed valve of the SRV attach well after the reassembly. 10. Install the pressure screw by turning it clockwise, and then secure it. 11. Put the sample probe through the center of the probe wipe, lift the probe wipe assembly back to the original position when the analyzer is turned off. CAUTION Make sure the sample probe is properly installed through the probe wipe and the probe wipe is at its original position. Otherwise the wipe may be stuck and unable to work properly. 12. Install the SRV waste collecting tray back properly, and then close the front cover. WARNING To avoid personal injury, when you close the front cover, be sure to hold it steadily before releasing the stop bar. CAUTION After the SRV tray is reassembled, check whether the connection between the SRV and the top of the sample probe is fixed. If not, tighten it to prevent air from coming into the probe and lead to unreliable analysis result. To avoid damage to the components of the analyzer, close the front cover gently.