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• Alternative to UF/alcohol • New prospecting strategies
UPCK™ Kit NuGel™ NRicher™ Mx
Urine Protein Enrichment & Concentration Low Abundance Proteome Enrichment
Linearly scaleable, unlike ultrafiltration Enriches & normalizes sub-proteomes
Alternative to solvent/ alcohol precipitation Compress proteome concentrations
On-bead digestion Species and tissue agnostic
protocols
Composite of the NRicher™ 6 mixed mode beads
<60 min. bind, Suitable for small volume mg scale preps
wash, elute
protocol Non-reduced SDS-PAGE Mouse Human
profiles show that major Plasma Serum
Applicable to high abundance bands are
>1 & 2 DE greatly reduced. The low
>LC-MS abundance bands are
>microarrays enriched relative to their
high abundance bands,
The eluted fractions visually estimated to be 5-
retain their enzymatic 10X for most bands.
and biological activity Because of its
heterogeneity, the IgG
variants co-localize to the
same region.
NRicher™ Mx
Eluates
AlbuVoid™ HemoVoid™
Selectively Voids Albumin, Binds Low Hemoglobin Depletion For Erythrocyte
Abundance Proteome Proteomics
Albumin voids in flow-through, >95% Hemoglobin voids in flow-through, applicable to red
cells, heavily hemolyzed serum, whole blood and
<30 minute protocol dried blood spot (DBS) card
Low abundance enrichment equivalent or better Low abundance protein and enzyme enrichment
than hexa-peptides or antibodies
Consumable, cost-effective
On-bead digestion protocols, efficient LC-MS
workflows Mild elution maintains native structure with
retained enzymatic, functional and bio-
Disposable, cost-effective, no column activities
regeneration or cross-contamination
Compatible with LC-MS, activity-probe profiling and
Mild elution maintains native structure with virtually all proteomic analyses
retained enzymatic, functional & bio-activities
Species agnostic
Species agnostic
AlbuVoid™ LC-MS On-Bead HemoVoid™ Blood Card Kit
Selectively Voids Albumin, Binds Low The HemoVoid™ Blood Card kit substantially reduces
Abundance Proteome before LC-MS use hemoglobin interference interference from dried blood
spot card protein analytes
Seamless workflows
Label, label free & glyco- compatible
Unique proteolytic efficiencies
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