P .Process Maturation

Step 1 Disinfection by using Sodium Hydroxide Solution NAOH

A. Fill the new water with every tank.

B. Prepare Sodium Hydroxide solution at pH 12 ( Conc. 0.2% ).

C. Spray the NaOH solution over the walls of the culture tank
and leave it for 1 hour.

D. Soak all the equipment's in the NaOH solution and leave for
1 hour.

E. Switch On the recirculation System and allow the NaOH
solution to pass or circulate in the total system.

F. Spread the solution on the floor and apply in Drain canal,
leave it for 1 hour.

G. Now drain out all the NaOH solution from the tanks and
pipes and clean with fresh water and run the pipes &
recirculation system with fresh water for 4 hours to remove
all the chemical solution.

Step 2 Disinfection by using Acidified chlorine solution

A. Drain out all the chemical water
and pass the system or run by
using fresh water.

B. Stock the culture tanks full with
new fresh water and prepare for
dechlorinating.

C. Take out all the equipment's
soaked and clean with fresh water
thoroughly and dry in the sunlight.

Step 3 Fungus Disinfection

A. Tank , wall and Floor
B. Mix 5 ppm of Treflan in fresh water
C. Spray on the walls and floor of the section.

Step 4 Fumigation

A. Chemical Preparation by using Formalin
and Potassium - permanganate
(KMNO4) in the ratio of 2 : 1

B. Calculate per volume of the room by
using Formalin 43 ml and Potassium
permanganate 22 grams per 1 ton of
the room volume ( Length X Width X
Height )

C. Close the room for 7 days after
disinfection.

Step 5 Disinfection by using Acidified chlorine solution

Preparation for Start the Section

A. Stocking fresh water full in the culture
tanks, washing all the area, walls , floor
etc.. to remove the chemical used.

B. Clean all the equipment's soaked with
fresh water and dry.

C. Prepare equipment and set aeration
system in the Brood stock culture tank.

Step 1 Management Reservoir Tank

A. Put chlorine powder 5-10 ppm. Or
liquid chlorine 15-20 ppm.

B. Open aerations 3-4 points/tank
C. Waiting chlorine 0 ppm. Can pump

next to Ozone machine.
D. Washing tank 1 time/month

Step 2 Disinfect with Ozone

A. Residual 0.4-0.6 ppm. Hours Residual
B. Half-life, Ozone must be 1 1 0.4
2 0.2
times reduced. 3 0.1
C. Normal ozone value 0.4 ppm. 4 0.05
5 0.025
It will take time. to break up 6 0.0125
within 8-10 hours. 7 0.00512
D. Should open the wind to be 8 0.000255
strong, will help the ozone run
out faster.
E. The house should have good
ventilation.

Step 3 HOD (Hydro-Optic Disinfection)

A. The water used must be very transparent.
B. Must control the conductivity at 300 MJ / CM or more
C. Medium pressure UV advantages
D. Broader wave length
E. Higher output per lamp (less lamps)
F. Not sensitive to water temperature
G. No repair effect of the microbes
H. Variable output (LP is ON/OFF)

Configuration - Control Modules Options Atlantium’s 10cm High intensity MP lamp

Step 4 UF (Ultra Filtration) o.o4 μ

A. Can filter the highest resolution at 0.04 microns
B. Filters change every 2-3 years according to the turbidity of the water.
C. The machine will automatically drain the water every 30 minutes.
D. Drain the water for 1-2 minutes at a time.
E. Keep the pressure in the normal state.
F. If the device is not working properly, the filter can be washed with

hydrochloric acid. Or every 6 month.

Step 5 Storage tank adjust new water

Chemical Other
A. DETA 10-15 ppm. (Precipitation A. Temp. 28-29 °C

of heavy metals) B. Turbidity >95%
B. NaHCO3 150-200 ppm. C. DO > 5 ppm.
D. Bacteria GVC = ND
(alkalinity use build shell) E. Bacteria TVC < x 102
C. Salinity 30-32 ppt.
D. pH 7.8-8.5 ppm.
E. Ozone 0.0 ppm.
F. Chlorine 0.0 ppm.
G. On UV

Step 1 Storage tank adjust new water

A. Clean the pond With chemicals according to the specified
concentration

B. Prepare water 500 lite 2 tank. Tank 1 put Providine 5,000 ppm. Tank
2 put freshwater only

C. Prepare water at the level of 40-60 cm. Depending on the
temperature of the water used to accept the breeder.

D. Floating bag adjust temperature in culture tank about 30 minute

Step 2 Check quality

A. Swab Brood stock and sampling water in bag. Cut legs for
checking PCR. Check IHHNV , WSSV, HPV, TSV, EHP.

B. In the morning, check for wound, death and take record.
C. Measure the brood stock length and weight

Step 3 Feeding program

Feed brooder immediately. After 3 day give fresh feed. Feed 7-9 meal/day

Squid feed 10-12%

Polycheataes (worm) feed 8-10%

Matplus feed 0.5-2%

Step 1 Eye talk ablation

A. For inhibition of ovulation-stimulating hormone.
B. For to break Gonad inhibiting hormone, that helps the ovaries

mature and widely used in the hatchery well.
C. For to help with faster spawning cycles.

Prepare equipment

➢ gas stove
➢ hook
➢ Scissors
➢ Povidine
➢ Gloves
➢ Shrimp Scoop Net

12 3

45 6

Step 1 Preparation Squid

A. Defrosting and Cleaning
B. To put it into a squid machine2-3 time.
C. Dissolve Astaxanthin in warm water 50-60°C
D. Squid ready to eat.

Step 2 Preparation Polychaeta

A. Adjust the water temperature to 60-70 °C
B. Leave for 2-3 minutes and filtered to remove the Polychaeta worm off.
C. Polychaeta worm ready to eat.

Step 2 Program feed

Feeding management per day

Time Type %feed

02:00 Broodster(BT) / Matplus(MP) 0.5%

06:00 Squid(SQ) 3%

11:oo Polycheates(PC) 3% % Feed using per day

15:oo Squid(SQ) 4% Squid (SQ) 10-12%
Polychaeta(PC) 8-10%
18:00 Polycheates(PC) 3% Brooder feed(BT) 0.5-2.0%
Mat plus (MP) 0.5-1.0%
21:00 Squid(SQ) 4%

23:30 Polycheates(PC) 3%

Step 1 Sourcing in Maturation

Sorting start 1:00 pm. And 3 pm. Sort 2 time/day. By use head light
select egg stage 4 ,can looking on back side shrimp. Select female have
sperm bag stick in center Thelycum.

Select sperm bag stick in center Thelycum. Big sperm bag give more
nauplius about 160,000-250,000 pcs./ spawn.

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✓ x x x ✓x

Step 1 Sourcing in Maturation

A. Wash females with Iodine 100 ppm. 0.5 minute.
B. Density female 1 pcs : water 250 lite. Temperature 29-31 °C
C. Stirring egg every 30 minute
D. After hatching off air.
E. Harvest nauplius1 and move to stocking room.
F. Stocking 1.5-2.5 mill./tank 500 lite (5,000pc./lite)
G. Transfer Nauplius 2 or 4.

Step 1 Harvest and Late Np.

A. Off air and on light for compile Nauplius.
B. Using hand net harvest Nauplius every 15-20 minute. Total keeping

finish 4-5 time.
C. Density 1.5-2.5 mill. / bucket / <2 hr.

On light for compile nauplius Late nauplius to bucket

Step 2 Counting and Transfer

A. Sampling random nauplius in bucket. By tube 5 ml. take to plate.
B. Counting and calculate nauplius.
C. Bill to nursery (Add 50%)