Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”




Thailand – Japan Student Science Fair 2020


Princess Chulabhorn Science High Schools are proud to organize the
th
Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020) on February 24 to
26 , 2021 at Princess Chulabhorn Science High School Trang.
th

This collaborative international event is under the motto of TJ-SSF 2020
“Seeding Innovations Through Fostering Thailand-Japan Youth Friendship”
which will be graciously presided by Her Royal Highness Maha Chakri
Sirindhorn who has been the driving force in the promotion of education to all
Thai youths, especially in learning of science, mathematics and technology.

The goal of this Thailand – Japan Student Science Fair 2020 is to bring
together groups of talented students in science and mathematics, from Thailand
and Japan to share and exchange their research finding and to build closer and
stronger collaboration between the two countries.

There will be thirty-four schools from Thailand, seventeen schools from
Super Science High Schools and twelve colleges from KOSEN participating in
TJ-SSF 2020.

The Thailand – Japan Student Science Fair 2020 will be a cordial bilateral
relation between Japan and Thailand. The event will not only provide a platform
for the exchange in scientific knowledge between these like-minded young
scientists but also will act as a springboard for a sustainable development and
promotion of many more 21 century skills which range from communication,
st
collaboration to long lasting friendships among youths.

Thank you all for your participating on this auspicious cooperation. We
hope you will have a pleasant stay in Trang, Thailand.

















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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Table of contents


Message from Minister of Education ........................................................... III
Message from Ambassador of Japan to Thailand ....................................... IV
Message from Deputy Minister of Education ................................................ V
Welcome Message from Secretary General of the Office of The Basic
Education Commission ................................................................................. VI
Keynote Speakers Biography ...................................................................... VII
Abstracts of Contributed Papers: Oral and Poster Presentation .................. 1
Section A : Mathematics ................................................................................. 2
Section B : Biology ....................................................................................... 19
Section C : Chemistry ................................................................................... 62
Section D : Physics ....................................................................................... 87
Section E : Computing and Information Technology .................................. 110
Author Index ................................................................................................ 127
Contributors for TJ – SSF 2020 .................................................................. 133


































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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Message from Minister of Education

Nataphol Teepsuwan

I would like to express my congratulations to Princess Chulabhorn Science
High Schools of Thailand, Super Science High Schools and KOSEN Institutes of
Japan, on their successful academic cooperation that leads to the Thailand-Japan

Student Science Fair 2020, (TJ-SSF 2020) which this year will be held during
February 24 to 25, 2021. Due to growing concerns over the coronavirus (COVID-
19), and with the well-being of participant, as our top priority, we have decided
that rescheduling the event is the only responsible action at this time and
organizing online participants of in Japan as well as students in Thailand.
It is a great pleasure to see Thai students and Japanese students learn
together which will definitely lead to the discovery of each other cultures for

fostering stronger friendship among the new generations of Thailand and Japan.
TJ-SSF2020 will also ensure the collaborative teaching and learning, including
building closer and stronger collaboration in education in in Science,
Mathematics and Technology between the two countries, as the motto of TJ-SSF,
“Seeding Innovations through Fostering Thailand-Japan Youth Friendship”.




Mr.Nataphol Teepsuwan,
Minister of Education
Ministry of Education, Thailand



















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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Message from Ambassador of Japan to Thailand


On behalf of the Government of Japan. It is a great pleasure to see the
Thailand-Japan Student Science Fair 2020, TJ-SSF2020 again, the first one, TJ-
SSF2015, was held 3 years ago then the second event, TJ-SSF2018, in 2018. It
clearly confirms the cooperation in Science, Mathematics and Technology

education between Thailand and Japan. I am pleased to see a significant progress
in having participation not only students from Super Science High Schools of
Japan, but also students from KOSEN Institutes of Japan.
I hope this TJ-SSF2020 will further encourage both Japanese and Thai
students to expand their opportunities in their further education in science and
technology to the development of our countries. May I express my appreciation

to an excellent arrangement and hospitality of the Thailand-Japan Student
Science Fair 2020, TJ-SSF2020, at Princess Chulabhorn Science High School
Trang.






H.E. Mr. Kazuya Nashida,
Ambassador of Japan to Thailand























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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Message from Deputy Minister of Education


Dr Khunying Kalaya Sophonpahnich

I would like to extend my warmest welcome to Japanese participants of
Super Science High Schools and KOSEN Institutes of Japan, and Thai
participants of Princess Chulabhorn Science High Schools as well as of other Thai
High Schools to the online Thailand-Japan Student Science Fair 2020 (TJ-
SSF2020). Thank you much indeed for your effort and cooperation.
I would like to sincerely thank the committee for organizing this academic
that provides opportunities for Thai and Japanese students to share knowledge of
their successful science projects.
As we are now living in a modern world where sciences, mathematics and
technology are profoundly embedded in our daily lives, educating young
generations in sciences, mathematics technology will give positive impacts on
their future.
The Thailand-Japan Student Science Fair 2020 with emphasis on sciences,
mathematics technology is expected that students will strengthen their knowledge
and skills through collaborative learning together.
The science projects in diffrent areas, such as Physics, Chemistry, Biology,
mathematics and Technology, I am sure that students will gain benefits from new

initiatives presented at this Science Fair. Also, I strongly believe that participating
in activities will enrich students’ learning experiences and sharing resources
among schools, enhance teaching methodologies and practices, and address
students’ needs and interests.
The organisation of Thailand-Japan Student Science Fair 2020 could not
have been possible without collaborative cooperation and support from Thailand
and Japan, especially our hosts, Ministry of Education, Thailand and Princess
Chulabhorn Science High School Trang. Thank you once again for your hard
work.
I hope that everyone of you will have fruitful and meaningful exchanges
during your stay and participation in the programme. Welcome and enjoy your
time in Trang.




Dr Khunying Kalaya Sophonpahnich
Deputy Minister of Education, Ministry of Education, Thailand


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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Welcome Message from Secretary General of the Office of The

Basic Education Commission


As the Secretary General of the Office of The Basic Education Commission
and the Chairman of the Steering Committee of Princess Chulabhorn Science
High Schools, I would like to welcome all participants, especially, Japanese
participants from Super Science High Schools and KOSEN Institutes of Japan to

the Thailand-Japan Student Science Fair 2020 (TJ-SSF 2020).

With the Coronavirus is still spreading around the world and recent
resurgence of Covid-19 cases in Thailand the Office of The Basic Education
Commission have shifted the Thailand-Japan Student Science Fair 2020 (TJ-SSF
2020) to the online platform.


The Thailand-Japan Student Science Fair emphasizes on the applications
of Science, Mathematics and Information and Communications Technology for
inspiring students to be young leaders with the evolution of technology for
becoming promising forces in driving economy and quality of life of the people
in both countries to the modern world.


I would like to take this opportunity to express our sincere thanks and
appreciation to Ministry of Education, Culture, Sports, Science and Technology
(MEXT) of Japan, Japanese and Thai Principals and Teachers special thanks PTT
Public Company Limited for supporting this academic cooperation of TJ-SSF
2020.







Dr. Amporn Pinasa
Secretary General of the Basic Education Commission










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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Keynote Speakers Biography


Takaaki Kajita is the Special University

Professor at TheUniversity of Tokyo, and also
the Director of the Institute for Cosmic Ray
Research (ICRR) of The University of Tokyo.
Kajita received his Ph.D. from The
University of Tokyo School of Science in 1986,
and has been researching at Kami-okande and
Super-Kamiokande detectors at the Kamioka

Observatory in central Japan.
In 1998, at the Neutrino International
Conference held in Takayama, Gifu, he showed
the analysis results which provided strong
evidence for atmospheric neutrino oscillations.

In 2015 he shared the Nobel Prize in
Physics for his role in discovering atmosphe-ric
neutrino oscillations.
Currently, he is the project leader for KAGRA Project, aiming to explore
the gravitational wave astronomy.


Education Graduated from Kawagoe High School 1977


Bachelor of Science, Saitama University 1981


Master of Science, The University of Tokyo 1983


Doctor of Science, The University of Tokyo 1986


Specialized Cosmic Ray Physics
Field
Career Research Associate, International Center for 1986-
History Elementary Particle Physics 1988
Research Associate, Institute for Cosmic Ray 1988-
Research, The University of Tokyo 1992
Associate Professor, Institute for Cosmic Ray 1992-
Research, The University of Tokyo 1995


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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Associate Professor, Kamioka Observatory, 1995-
ICRR, The University of Tokyo 1999
Professor, Research Center for Cosmic Neutrino, 1999-
ICRR, The University of Tokyo Present

Director, Research Center for Cosmic Neutrino, 1999-Mar.
ICRR, The University of Tokyo 2016
Director, Institute for Cosmic Ray Research, The 2008-
University of Tokyo Present
Special University Professor,The University of Jan. 2016-
Tokyo Present

Distinguished Professor,The University of Tokyo Mar.2017-
Present
Council Member of Science Council of Japan 2017-
(SCJ) Present
Director, Next-generation Neutrino Science Oct. 2017-
Organization Present
Membership of the Japan Academy 2019


President, Science Council of Japan 2020

Awards and Asahi Prize (As a member of the Kamiokande 1988
Prizes Collaboration)
Bruno Rossi Prize (As a member of the 1989
Kamiokande Collaboration)
Asahi Prize (As a member of the Super- 1999
Kamiokande Collaboration)
th
The 45 Nishina Memorial Prize 1999

W.K.H. Panofsky Prize (with Drs. Masatoshi 2002
KOSHIBA and Yoji TOTSUKA)
st
The 1 Yoji Totsuka Prize 2010

Japan Academy Prize 2012


Julius Wess Award 2013

2016 Breakthrough Prize in Fundamental Physics 2015
(with Super-Kamiokande collaboration)


The Order of Culture 2015




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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Person of Cultural Merit 2015


The Nobel Prize in Physics 2015 2015


Chunichi Cultural Award 2016

2016 Harvard Foundation Scientist of the Year 2016


The 37 GITAM Foundation Annual Award 2017
th
2017
Berkeley Japan Prize 2017


IUPAP-TIFR Homi Bhabha Medal and Prize 2019

Honors Fellow – Saitama University 2015


Honorary Member – The Physical Society of 2016
Japan
Honoris Causa, Doctor of Science: Universidad 2016
Mayer de San Andres
Honoris Causa, Doctor of Science: Padova 2016
University
Honoris Causa, Doctor of Science: Aligarh 2016
Muslim University

Honoris Causa, Doctor of Science: The 2017
University of Naples Federico II
Honoris Causa, Doctor of Science: The 2017
University of Perugia
Honorary Professor: The University of Delhi 2017

Honoris Causa, Doctor of Science: Veltech Dr. 2017
RR & Dr. SR University
A member of the Paseo de las Estrellas de la 2017
Ciencia de La Palma

Honoris Causa, Doctor of Science: The 2017
University of Bern
Honoris Causa, Doctor of Science: Amity 2018
University
Honorary Professor: University of Electronic 2018
Science and Technology of China
Honorary Professor: Sichuan Normal University 2018



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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Honorary Professor: Chengdu University of 2018
Technology
Special University Professor,Toyama University 2019


Honoris Causa, Doctor Philosophiae: The 2019
National Academy of Sciences of Ukraine

Honorary Professor: Wuhan University 2019


Wang T.C. Lecture Professorship: Wuhan 2019
Institute of Physics and Mathematics, the Chinese
Academy of Sciences
Foreign Member of the Russian Academy of 2019
Sciences
Honoris Causa, Doctor of Science: University of 2019
Dhaka
Selected “Evidence for an oscillatory signature in 2004
Papers atmospheric neutrino oscillations” Phys. Rev.
Lett. 93
“Observation of Atmospheric Neutrinos” Rev. 2001
Mod. Phys. 73


“Evidence for Oscillation of Atmospheric 1998
Neutrinos” Phys. Rev. Lett. 81

“Atmospheric Muon-Neutrino/Electron-Neutrino 1994
Ratio in the MultiGeV Energy Range” Phys. Lett.
B335
“Observation of B-8 Solar Neutrinos in the 1989
Kamiokande-II Detector” Phys. Rev. Lett. 63


“Experimental Study of the Atmospheric 1988
Neutrino Flux” Phys. Lett. B205


“Observation of a Neutrino Burst from the 1987
Supernova SN1987A” Phys. Rev. Lett. 58










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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Keynote Speakers Biography




Profile


Assoc. Prof. Dr. Anchana Prathep
Faculty of Science, Prince of Songkla University
E- mail : [email protected]

EDUCATION
Year of graduated Degree University
2001 Ph.D. Biological Science The University of Liverpool, United
Kingdom
1994 B.Sc. Biology Prince of Songkla University,
Thailand

SCHOLARSHIP:
: Thai Government Scholarship for Ph.D. (1997-2001)

: JICA Training course: Conservation of Wetland Ecosystems and Their
Biological Diversity (July 2002- August 2002)
: NRCT-KOSEF: Research Collaborations at Sungkyukwan University and
Wongkwang University (October 2005, 10 days)
: Global Taxonomy Initiative (GTI) Belgium: Ghent University, Belgium (July

2006- September2006)
: Higher of Education Commission: Research collaborative Initiative,
SeagrassNet University of New Hampshire, USA and Smithsonian Institute,
Panama (October 2009, November 2009)

CAREER HISTORY:
: Dean of Faculty of Science: June 2019- current

: Direct of Excellence Centre for Biodiversity of Peninsular Thailand: 2017-
current
: Vice Dean in Research and Postgraduates: 20012-2016
: Deputy Head of Biology Department: 2011-2012
: Technical Expertise: Biodiversity, Ecology, Seaweed and Seagrass




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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Abstracts of Contributed Papers: Oral and Poster

Presentation




Oral and Poster Presentation
Section A : Mathematics

Section B : Biology

Section C : Chemistry

Section D : Physics

Section E : Computing and Information Technology




































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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
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Oral and Poster Presentation

Section A : Mathematics

Calculating the parallels distances using a moiré patterns and a rotated
patterns

Nonnaphat Natpanyaporn , Ronnakon Nisaensat , Wasuphon Moonmanee
1
1
1
Advisor: Phiphat Chuamuangphan
1

Princess Chulabhorn Science High Shool Chiang Rai
1


Abstract


Moiré pattern or Moiré fringes is the phenomenon that occurs when two parallel patterns of
parallel lines have the same width of a gap for each line. The pattern is superimposed, causing the light
to intervene which creates light and dark shaded areas. This phenomenon can be used to find the
number of lines in the parallel pattern by placing a different size of lunormeter on the threads that we
want to measure using the rotated pattern formula to calculate.

The condition of the rotated pattern formula is the parallel patterns have to have the same width
of a gap for each lines to create the moiré pattern and can conclude that the number of lines in a parallel
pattern are equal to the number of lines in the lunormeter .Lunormeters are expensive, so the creators
have an idea to apply the rotating pattern relativity formula by using the gap width fixed parallel
pattern to calculate the unknown width gap parallel pattern. From that, we’ll get




From testing the formula to calculate the parallel lines compared to the reality occurred
by the parallel lines with the frequency 50, 80 and 100 lines per 100 square centimeter and the angle
between two parallel patterns equal to 2.5, 5.0 and 7.5 degrees.
Have an error between the formula and the reality equal to 0.320033586 line.


Keywords: Moiré pattern, rotated pattern, and lunormeter.














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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Increasing Flow Rate Model via Helicoid Equations for Removing
Blood Clots in Medical Treatments


Patcharapol Rujipornpong , Pittinan Harnsinghkun
1
1
Advisors: Pitsinee Kongsukon , Wasan Akarathanawat
1
2

Princess Chulabhorn Science High School Phetchaburi
1
Faculty of Medicine, Chulalongkorn University
2

Abstract


Mechanical Thrombectomy, an efficient Venous Thrombosis treatment, is the interventional procedure
of removing blood clots from blood vessels in patients with Venous Thrombosis. This removal
is achieved by using helicoid to transfer the blood clots. The removal of blood clots increases
the blood flow rates when patients have had effective helicoid treatments. This project aims to use
helicoid equations to increase the flow rate of blood clot removal. We studied 3 types of helicoids
(circular, conical and paraboloidal helicoids) with surface integrals to derive new formulas.
These formulas were related to both the pitch and surface area of the helicoids, so that we could then
design treatment device models. Once the treatment devices were designed, we used Solidworks
Simulations to model, simulate and evaluate the flow of blood clots in the helicoid in both the head and
the body. The results show that in helicoids which had the same pitch, the blood flow rates
of paraboloidal, conical and circular helicoid are 1.006, 0.961 and 0.911 ml/s,
respectively. Furthermore, in helicoids which had equal surface area, the blood flow rates of
paraboloidal, conical and circular helicoid are 1.098, 1.012 and 0.911 ml/s, respectively. To
summarize, our results demonstrate that paraboloidal helicoid with equal surface area is the most
appropriate helicoids for their capabilities to increase the flow rate of removing blood clots, at a
significance level of .01.

Keywords: Helicoid Equation, Pitch, Surface Area, Blood Clot, Mechanical Thrombectomy




















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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Safety vault with security sharing algorithm using matrix


Natthanan Phutthason , Patipan Nillaphant
1
1
Advisor: Sumalee Klaimuang
1

Princess Chulabhorn Science High School Loei
1



Abstract


Mathematics Project on the topic of “Safety vault with security sharing algorithm using matrix”
aims to create a safe and create a password with Security sharing system by applying knowledge of the
matrix, the Geometer's Sketchpad (GSP) version 5.06 program and programming on the Raspberry pi
3. It has been summarized and discussed the results as follow;
From the study of how to create a security system Pattern for sharing secret passwords and
creating safes with security systems by using mathematical knowledge about matrix was found that the
secret t out of n then t, n I+, and has 5 patterns. There are a format for sharing
the secret 2 out of n, 3 out of n, 4 out of n, 5 out of n and 6 out of n. In calculation and build a safe with
a secret-sharing security system using the raspberry pi 3 board to control the mechanism of the safe by
having guidelines for creating security systems. The format for sharing the secret t out of n is as follows:

If is password and divide the information related to the secret into n parts, then give each section
to relevant parties. When taking any part of the confidential information t in this n part together. It will

be able to find . But if there is less confidential information than t, it will not be able to know
with the following steps.

Then t I+ and

n I+ and

1. Select function and if is password then

.

2. Choose n points that are on the curve that from

relevant parties then n is .
3. Substitute at least t points to create linear equations and get a system of linear equations,
t equations, variables as follows:

From

From



From



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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”




Then are unknown variables.
4. Change the system of linear equations t variable equations into the form of matrices as
follows:











5. Solving systems of linear equations Using Cramer’s rule as follows:






6. Substitute in and find then

.

Keywords: Security sharing system, Polynomial function, Matrix, System of linear equations,
Raspberry Pi 3





























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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Finding a relationship between the number of vertices of a regular polygon
inscribed in a circle and the number of regions made
By its perimeter and diagonals



Supakorn Thepchan , Samonrach Sutthiboon ,
1
1
Advisors: Maitree Somboon , Detchat Samart
1
2

Princess Chulabhorn Science High School Chonburi
1
Department of Mathematics, Burapha University
2


Abstract

In 1998, B. Poonen and M. Rubinstein established a correct relationship between the number of vertices
of a regular polygon inscribed in a circle and the number of regions formed by its perimeter and
diagonals using advanced techniques from algebra and geometry. The aim of this research is to explain
this relationship using an easy approach. In our study, we use GSP program to illustrate and count the
number of regions made by the perimeter and diagonals of a regular polygon. Based on our observation,
the relationship between the number of regions and the number of vertices, say n, of a polygon can be
separated into 2 main cases depending on parity of n. When n is odd, we find that the number of regions
is n4-6n3+23n2-18n+2424, which agrees with the result of Poonen and Rubinstein. When n is even, we
consider the two subcases, depending on whether n is a multiple of 4. If n is a multiple of 4, we
hypothesized that the number of regions is 9n3-72n2+176n 16. If n is not a multiple of 4 (except n=2),
the number of regions becomes 9n3-72n2+188n16. However, the last two formulas are valid only for
even number n from 4 to 14. To obtain a correct formula for larger even number n, one might need to
consider more possible cases.


Keywords: vertices, a regular polygon, diagonals, circle, the number of regions





















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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The rounded shape with sides were formed by Hypocycloid curve


Chanikarn Detchsong , Piyachat Wongnurak
1
1
Advisors: Kanda Kaosumpun , Kittipol Nualtong 2
1

Princess Chulabhorn Science High School Trang
1
Prince of Songkla University
2



Abstract

The propose of this project is to create the rounded shape with sides which were formed by
Hypocycloid curve , find the area and the perimeter formula by creating geometry from Geometer’s
Sketchpad (GSP). We can prove that , the area formula of the rounded shape with sides is









and the perimeter of the rounded shape with sides is



where is the number of sides of the rounded shape and
is the number of spire of hypocycloid or sides of hypocycloid ,


Keywords: Hypocycloid





















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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Proving the product of four consecutive natural numbers is not a perfect

square by greatest common divisor and modulo
Witchaya Wittayasetthakun , Naphat Promprasert
1
1
Advisors: Supamit Wiriyakulopast , Nopporn Thamrongrat
2
1

Princess Chulabhorn Science High School Nakhon Si Thammarat
1
Walailak Univerisity
2



Abstract


In this mathematical project we study the product of four consecutive positive integer numbers is not a
perfect square . We separate proof in two cases are (n - 1 , n + 2) = 1 and (n - 1 , n + 2n) = 3 where n
2
2
2
2
∈ Z for n ≥ 2. In case (n - 1 , n + 2) = 1 we use n ≡ 0,1 (mod 4) to solve. In case (n -1 , n + 2) = 3 is
+
2
2
2
2
2
divided into two cases, for gcd (p, 9p + 18p + 11p +2) = 1 we use n ≡0,1,4,9(mod16) to solve and case
3
2
2
gcd (p, 9p + 18p + 11p + 2) = 2 is proved by comparative inequality. So, we can conclude that the
3
2
product of four consecutive positive integer numbers is not a perfect square.

Keywords: Non-perfect square






























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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Study of the Relationship of Spheres at overlapping that are packed in a

Cylinder

Sudarat Prakongsuk , Suphitchaya Khamanek ,
1
1
Advisor: Chatchadaporn Prachanpol
1

Princess Chulabhorn Science High School Buriram
1




Abstract

Mathematics Project Subject name is "The Study of the Relationship of Spheres at overlapping that are
packed in a Cylinder" is a project created with the objective to study the relationship between spheres
and cylinders and to find formulas that are used for calculate the size of a cylindrical cylinder that can
fit a large number of spheres. The researcher will study the relationship of spheres and cylinders, in
which the researcher will draw a spherical image that is packed in a cylinder in 2D. Define variables
from the drawing and the study method is divided into 7 cases, in which the researcher determines the
diameter. And the researcher finds the formulas that are used to calculate the size of a cylindrical
cylinder that can fit a large number of spheres. The researcher will draw a spherical image that is packed
in a cylinder in 2D and define variables from the drawing. After that, studying and collecting data. The
experimental results are the relationships of the diameter and the cylinder between the height of the
cylinder in the ratio which is in the form of a circle diameter. To get the formula that is used to calculate
the size of a cylindrical cylinder that can fit a large number of spheres. The method of operation is
applied the knowledge of the Geometry and the Pythagorean theorems; To solve everyday problems,
To make it more convenient and easier. This project will be a small point to create a new innovation in
quality of life in the future.

Keywords: Relationship of Spheres, Cylinder






















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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Finding the relationship of a curved pattern from the substitution of x
by the positive integer in the Fibonacci polynomial function


Kwanchanok Siritan , Chananchida Sudsa-ard
1
1
Advisors: Chitpong Neuakorwai , Daoruang Butsap
1
1

Princess Chulabhorn Science High School Pathumthani
1





Abstract

The purpose of this study is to create a curved pattern relationship by substituting x with a
positive integer in the Fibonacci polynomial function. To be applied in the pattern design and building
as a guideline in the design of products or decorations. Beginning with the study of the form of the
Fibonacci polynomial and analyze the relationships of each number sequence that result from
substituting x values with positive integers in the Fibonacci polynomial function. After that, take the
resulting numbers and create a relationship in the form of curves associated with the number sequence
generated by the Fibonacci polynomial function. By creating curves based on the arrangement of
isosceles and equilateral triangles, which have side lengths in the order of number sequence produced
by the Fibonacci polynomial function and considered the ratio of the side lengths of each of the
connected triangles. And create the equation for the length of a curve in a general form. Which is come
from a circle surrounds an equilateral triangles n triangle, which has lengths from each number
sequence, which is caused by substituting x with the integer into the Fibonacci polynomial function by
determine the range of the numbers from 1 to n, it is found that the ratio of the lengths of each triangle
caused by substituting x with one positive integer in the Fibonacci polynomial function will be
following one of the Metallic means produced by the x order, and the general curve length can find
th
from the equation Ls=239(1na) by a is the length of equilateral triangles that follow the Fibonacci
sequence and n is the number of equilateral triangles. And able to use the resulting curves to create a
further image.

Keywords: Fibonacci polynomial function, Fibonacci sequence, Metallic means

















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Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Study of the Relationship between Return and Investment Risk
by Using Game Theory.


Thittawat Unta , Chatpakorn Tuncharorn
1
1
Advisor : Angsananun Densathan
1

Princess Chulabhorn Science High School Phitsanulok
1



Abstract


The objective of this project is to study the relationship between return and investment risk in
the common stock on the Stock Exchange of Thailand by using game theory to explain the relationship.
The results showed that there are important factors that affect on return and investment risk such as the
company's performance, related industry conditions and economic conditions which will be able to
measure the company's performance from complete net company profits, industrial conditions from the
number of competitions and economic conditions from the SET Index. If the return is high and the risk
is low, then the net profit will be complete, the number of competitions will be lower and the economy
will be good for that company. The return on investment in the common stock in the Stock Exchange
of Thailand, is dividend and capital gain from the sale of the stock, and defines the risk of investing in
the common stock in the Stock Exchange of Thailand which is capital gain in loss from the sale of
stock. The relationship of return and risk to invest in equation when the equation is analyzed together
with the definitions of both parts and transformed into equations along with mathematical operations,
then will find that when the return is combined with the risk will come out as a dividend definition. If
using in the calculation, replace the dividend yield variable in percentage and when using game theory
to analyze the relationship between return and risk in competitive investment. The result of the analysis
is that if Rt-Rk is greater than -Rt, it means that you should invest in the common stock of that company.
Unless if the result is less or equal, then it should not be invested with the common stock of that
company which will be able to help guide the decision to buy the common stock in the Stock Exchange
of Thailand.
Keywords: Return on Investment, Risk on Investment, Common Stock, Game Theory



















11

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



A study of equations for showing double cone movement on the plane that
increase the slope

Pornpit Wasuwan , Phatsamon Wannakan
1
1
Advisor: Sunsanee Boonsanit
1


Princess Chulaborn Science High school Mukdahan,
1




Abstract

This project is the study of equations for double cone that can move automatically on rails with
increasing slope. Most of 3D geometry shapes usually move following gravity but double cone has
different shape from them. We change radius of double cones to find the relationship between the double
cones and apply the general formula. We create the project to find the relationship of double cone by
using trigonometry and consume the result to improve creativity and imagination. We determine the
angle between the rail and ground, 2β for angle of rail and γ for angle inside the cone.

After the calculated conducting, the relationship of equation is tan <tan⁡(β) tan⁡(γ). The
value of radius that can find the value of tan ,tan , tan⁡(γ) are between 2-13 centimeters. Moreover,
the value of 3 angles must according to the relationship in order to moving on the increasing rail.


Keywords: double cone, radius, tan ,tan , tan⁡(γ)



























12

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”




A Mathematical Model of Dengue hemorrhagic fever in Lopburi province
Kanokporn Paleeta , Itchaya Boonnak
1
1
Advisors: Watcharee Chanpuak , Amornrat Uantrai
1
2

Princess Chulabhorn Science High School Lopburi
1



Abstract

Dengue is a viral infection which is carried by Aedes mosquitoes that are found in tropical and warm
areas. Because of a tropical climate, the disease has become a widespread problem in Thailand.
According to the research, there are patients with dengue every year in Lopburi province. Each year,
the number of patients is not stable, so the researchers have created A Mathematical Model of Dengue
hemorrhagic fever in Lopburi province to analyze the factors affecting the dengue epidemic. Using
mathematical models as a tool leads to solutions to the dengue epidemic. The analysis showed the
factors that affect the dengue outbreak related to the self-care behavior of people in Lopburi province
and environmental factors. The dengue epidemic data are analyzed using the value of and .

Keywords: Dengue hemorrhagic fever































13

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



X-intercept Distance for the Tangent Line of Circle and Ellipse



Shoko Yothee , Husna Aujeh
1
1
Advisor: Anan Chantarat

Princess Chulabhorn Science High School Satun
1
National Center for Genetic Engineering and Biotechnology, National Science and Technology
2
Development Agency, Pathumthani





Abstract

The purposes of this study were to find out the relationship between X-intercept distance of
tangent line with the distance from the center of the circle to the projection of the tangent points on the
circle and the radius of the circle. The relationship between X-intercept distance of tangent line with
the distance from the center of the ellipse to the projection of the tangent points on the ellipse and the
length of major axis and minor axis of the ellipse.

Performance shows that

1. For any circle, that there are the length of radius equal to r, the center is (0,0) , the tangent line
intersects the X-axis at (x2,0) and the projection of the tangent point on X-axis at (x1,0), we can conclude
that r = x1x 2
2
2. For any ellipse with parallel axes parallel to an X-axis, that there are the length of major axis equal
to r and the length of minor axis equal to s, the center is (0,0) , the tangent line intersects the X-axis at
(x2,0) and the projection of the tangent point on X-axis at (x1,0), we can conclude that r = x1x2
2
3. For any ellipse with parallel axes parallel to an Y-axis, that there are the length of major axis equal
to r and the length of minor axis equal to s, the center is (0,0) , the tangent line intersects the X-axis at
(x2,0) and the projection of the tangent point on X-axis at (x1,0), we can conclude that s = x1x 2
2

Keywords: X-intercept, Tangent line
















14

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Ingenuity of Krathong Designs Using Polygons and Pascal's Triangle


Sirikorn Chanvilainakorn , Tantai Ruenruay
1
1
Advisors: Janjira Khamnan , Dino Alfaro Molano
2
1

Takpittayakhom School
1
Abstract


The purposes of “The Ingenuity of Krathong Designs Using Polygons and Pascal's
Triangle”project are: 1) To study the relationship between forms regular polygons constructed on a
circle and Pascal triangle by using the Geometer’s Sketchpad program (GSP). 2) To design the patterns
and creating a decorative Krathong Kala. By integrating mathematical knowledge on binomial theorem,
combination rules and Pascal’s triangle in order to find patterns which were made by creating regular
polygons and all points on the circle from 1 to 7 points by using Geometer’s Sketchpad program then
to design patterns.
1. In finding the relationship between form of regular polygons which constructed on a circle and
Pascal triangle by using Geometer’s Sketchpad program, when n is an element of counting number
result that:
 The number of points on circle has a relation in the second diagonal of Pascal’s triangle,



starting from 1, 2, 3, …, , ...
 The number of line segments has a relation in the third diagonal of Pascal’s triangle,



starting from 1, 3, 6, 10, 15, …, , …
 The number of triangles has a relation in the fourth diagonal of Pascal’s triangle, starting


from 1, 4, 10, 20, 35, …, , …
 The number of quadrilaterals has a relation in the fifth diagonal of Pascal’s triangle,


starting from 1, 5, 15, 35, …, , …
 The number of pentagons has a relation in the sixth diagonal of Pascal’s triangle, starting


from 1, 6, 21, 56, …, , …
 The number of hexagons has a relation in the seventh diagonal of Pascal’s triangle,


starting from 1, 7, 28, 84, …, , …
 The number of heptagons has a relation in the sixth diagonal of Pascal’s triangle, starting


from 1, 8, 36, 120, …, , …
2. In designing the patterns and decorating Krathong Kala by using worked from design, we gained
5 different patterns of Krathong Kala.
Keywords: Polygons, Pascal's Triangle


15

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Some properties of inscribed relation in regular n-gons

Supakit Krailert , Pimlaput Olrankijanun
1
1
Advisor: Nattawoot Doomluck
1

Phuketwittayalai School
1





Abstract


Some properties of inscribed relation in regular -gon begins with considering regular
polygon inscribed in regular polygon. We use knowledge in Mathematics and Technology in order
to find the theories and prove some properties of regular polygon inscribed in regular polygon
from number theorem. We also use DEV-C++ program to calculate for finding duplicated vertices.
From considering regular polygon inscribed in regular polygon, we bring about the conditions of

regular polygon inscribed in regular polygon if and only if and find that any regular
polygon inscribed in regular polygon has some duplicated vertices if and only if

when is the first vertices, is the interval between the vertices

of any and the number of duplicated vertices is equal to . Furthermore, we can
also find how to calculate the number of areas that result from the blockage of regular polygon or


regular polygon. If the different vertices of regular polygon that are supported by any sides of

regular polygon are equal to where , the number of areas that result from the blockage of
regular polygon or regular polygon where that side of regular polygon passes is equal

to areas. In addition to these results, we can find that if we color the areas that result from the
blockage of regular polygon or regular polygon, the least number of colors is equal to 2.

Keywords: regular polygon, duplicated vertices, the number of duplicated vertices,
congruence equations, DEV-C++ program, face, chromatic number















16

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Extension of the Pizza Theorem to Any Regular N-polygon

Proof Using Internal 2N-Polygon and External Symmetry


Mai Nozaki
1
Advisors: Hiroki Iwakura , Rick Mabry
1
2

Bunkyo Gakuin University Girls’ Senior High School
1
Louisiana State University
2



Abstract

The pizza theorem states that if a circular pizza is divided into 8, 12, 16 or more slices by
making cuts at equal angles from an arbitrary point, then the sum of the area of alternating slices (every
other slice) is equal. Here, I prove that the pizza theorem can be extended from a circle to determine the
area of any regular N-polygon. The demonstration was performed in four steps.
In the first step, a 2N polygon (hereinafter referred to as "Cutting Polygon") is created inside
the regular N polygon by a certain operation. Then, in the next step, prove the joint definition in the
pizza theorem in Cutting Polygon. In the third step, the area between the regular N-sided polygon and
the 2N-sided polygon (hereinafter referred to as "External Cutting Polygon") is cut by a certain
operation, and a pair of pieces named based on the symmetry used in proof are created. Then, in the
final step, demonstrate the congruence definition in the pizza theorem for a pair of External Cutting
Polygon pieces using "symmetry" and "rotation of the cut line".
As a result, it was proved that the pizza theorem can be extended from a circle to a regular N-
polygon in the case where N is even.

Keywords: The pizza theorem, geometry, N-polygon, symmetry,























17

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Buffon’s leaf problem
Approximating pi using fallen leaves


Takano haruka
1
Advisors: Kida Hideaki , and Matsuda Keisuke
1
2

Hiroshima University High School, Hiroshima, Japan
1
Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
2
Osaka University Hospital, Osaka, Japan
2





Abstract

“Buffon’s needle problem” is one of the most famous math problems. Buffon proved that when needles
of length l are randomly thrown on parallel lines placed at regular intervals of d, the probability of the
needles to cross any of the lines is equal to 2l/πd. The theory can approximate the value of π by counting
the number of needles that cross the parallel lines. Based on this theory, we have been trying to build
a theory which can approximate the value of π by fallen leaves on a brick pavement. To reach our goal,
there are two tasks. One is that leaves have diversity in their shapes. To find how our theory can deal
with the diverse shapes, we have formulated an equation to approximate the value of π by throwing
needles of varied lengths, and we confirmed the validity of the equation based on experiments and
simulation programs. The other is that a leaf is a more complex figure than a needle is. To find the
probability for leaves to cross a line when thrown on parallel lines, we proved that the convex hull of a
concave polygon crossing a line is the necessary condition for the concave polygon to cross a line and
found the probability for regular n polygons to cross a line based on a previous study.


Keywords: Buffon’s needle problem, probability, approximate, π, fallen leaves, convex hull, concave
polygon




















18

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”




Oral and Poster Presentation
Section B : Biology


Test of the efficacy of crude extracted from betel leaves to the
control
rice blast disease in rice field.


Chonlada Chaiyotha , Jirathip Saeoui
1
1
Advisor: Nurhidayah Kahong
1

Princess Chulabhorn Science High School Buriram
1




Abstract
Rice is an important economic crop and is a staple food for 2.7 billion people in Asia. But the problem
is the epidemic of rice that is heavily caused by rice blast disease by Pyricularia oryzae. Prevalent in
many countries such as Japan, Vietnam, Korea and many countries in Asia. Including Thailand, has
spread in every area throughout the country causing not being able to control the spread of the infection
as well as it should. From the comparison of the efficacy of crude extracted from between betel and
guava leaves to inhibit the growth of the rice blast disease the findings indicated that betel leaves had
more effective inhibition than guava leaves. Which can inhibit 100% of fungus of rice blast disease at
concentrations of 1000 ppm and 1500 ppm. And crude extracted of betel leaves use 95% ethanol as a
solvent, with the lowest concentration that can inhibit the infection 100% is 100 ppm is more effective
in inhibiting the growth of rice blast disease better than the crude extracted from betel leaves use water
as a solvent with the lowest concentration that can inhibit the infection 100% is 500 ppm. And when
tested in the control of rice blast disease in the field can reduce the incidence of disease from level 3 to
level 1. And the storage of crude extracted from betel leaves at 4°C and 28°C can remained effective
and the physical characteristics remained unchanged. And the chemical composition of the crude
extracted from betel leaves is Eugenol. From the above information, it can be developed into a product
to the community. And to encourage farmers to use local herbs to maximize benefits. It also helps to
reduce the import of chemicals and protect the environment contamination in another way.


Keywords: rice blast disease, crude extracted from betel leaves, Pyricularia oryzae












19

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Development of Bacterial Nano Cellulose Wound Dressing Sheet Coated
with Peka Leaf Extract for Prevention and Treatment of Infected Wounds


Pichsinee Rattanaphan , Phetlada Rungruang
1
1
Advisors: Kuntida Khansuk , Pornvichai Temboot
1
2

Princess Chulabhorn Science High School Nakhon Si Thammarat
1
Thaksin University
2



Abstract
The objective of this study was developed and evaluated the bacterial nano cellulose wound dressing
sheet coated with Peka leaf extract for prevention and treatment infected wounds. The effect of different
carbon sources, including old coconut water, young coconut water, marinade-citrus fruits, marinade-
sweet fruit and water from Nypa palm, on forming of bacterial nano cellulose were also determined.
Then, the physical properties, strength, and water absorption property, of bacterial nano cellulose sheets
were tested. We founded that the bacterial nano cellulose cultured from marinade-citrus fruit exhibited
the highest tear resistance at 14.89 newtons per 30 square centimeters. Furthermore, the bacterial nano
cellulose cultured in from marinade-citrus fruit showed the highest water absorption property with an
average of 60.59 percent. Then, we selected the bacterial nano cellulose cultured from marinade-citrus
fruit mixed with gelatin to develop the wound dressing sheets coated with Peka leaf extract and test
their antimicrobial activity to Staphylococcus aureus by disc diffusion technique. We founded that the
concentration at 50% (w/v) of Peka leaf extract coated on this sheet showed an average inhibition zone
of 12.22 ± 0.22 mm.


Keywords: Bacterial nano cellulose wound dressing sheet, Peka leaf extract, Antimicrobial activity















\





20

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Micro-plastic Degradation by Bacteria from Intestines of Mealworms


Chayaphol Laotaweerungreung , Pasit Soongying , Parin Jatesiktat
1
1
1
Advisor: Nattapon Klinput
1

Princess Chulabhorn Science High School Phetchaburi
1



Abstract
Seventy years ago, plastic waste has increased more than 200 times of each year. Improper disposal of
plastic waste has caused micro-plastics to spread in the environment and to harm ecosystems. The
project aimed to study the micro-plastic degradation by bacteria in intestinal mealworm, and to store
bacteria in moving bed biofilm reactor (MBBR) for decomposition of the micro-plastics. Mealworms
are fed by polyurethane foam (Polystyrene, PS) for fourteen days. Incubated intestines’ mealworms by
using Liquid carbon-free basal medium (LCFBM) and PS film at 24 C, then shook for fourteen days in
o
order to get PS degradable bacteria. Cultured bacteria with the agar medium to observe the colonies.
After that, incubated with MBBR at 24 C and shook for 7 days. Finally, froze dry so that it can be
o
stored for a long time. In order to examine the stored bacteria could decompose PS, the moving bed
with stored bacteria for 7 days have been tested for PS film decomposition. The results showed that the
average values of contact angle and surface tension in PS film of experimental group and control group
were decreased by 4.33 and 0.2 degrees, respectively. This result indicated that the bacteria in the
intestinal tract of mealworms could decompose Ps film even after being freezing dry and long-term
storage in the MBBR at -20 C for seven days. This study could be used to decompose micro-plastics in
o
water source that could not directly eliminated by mealworms

Keywords: Bacteria, Mealworms, Contract angle, Micro-plastic





















21

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Mapping of Seagrass Meadows Using Aerial Photography
at Pak Klong Bay in Trang Province, Thailand


It Jubprung , Rattanakon Phlunang
1
1
Advisors: Patchara Pongmanawut , Anantanit Chumsri
2
1

Princess Chulabhorn Science High School Trang
1
Rajamangala University of Technology Srivijaya Trang Campus
2






Abstract
There are several factors behind reduction of dugong numbers, and one of the responsible factors is
reduction of seagrass bed. More research should be conducted to know the present status of seagrass
bed in Thailand, as well as to conserve them. Researchers usually observe seagrass beds by sampling
information directly from the field, which is very difficult and time consuming. The aims of this study
are to (1) compare the seagrass coverage between field observation and aerial photography and (2) to
make seagrass mapping at Pak Klong Bay, Trang Province, Thailand. We divided it into three study
areas. Each study area was 30 x 30 meters and divided into 9 points. We surveyed seagrass using field
observation and using drones. Afterwards, calculated the percentage of each seagrass using Wolfram
Mathematica 11.3 program and analyzed the seagrass coverage (%) data using SPSS 22. We found three
seagrass groups in this study: Enhalus acoroides, long flat leaves and short oval leaves. In aerial
photography method using drones, we found 29.04% seagrass (19.48% of long flat leaves, 8.56% of
short oval leaves, and 0.99% of E. acoroides) and in field observation method using quadrats, we found
25.03% seagrass (16.43% of long flat leaves, 8.46% of short oval leaves, and 0.14% of E. acoroides).
There was no significant difference in seagrass coverage between aerial photography and direct field
observation methods. So, drone can be used for checking seagrass bed. This method is easier because
it needs few people, one drone and few hours to finish the study. After that, we surveyed seagrass by
aerial photography using drones to map seagrass at Pak Klong Bay, Trang Province, Thailand. We
found 11.84% seagrass in an area of 22,500 square meters (9.59% of long flat leaves, 2.21% of short
oval leaves, and 0.05% of E. acoroides).

Keywords: Aerial photography, drones, field observation, seagrass coverage














22

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Biofilm from Galangal to Protect Aspergillus flavus in Chopsticks


PaphichayaWarapho , Pattawan Ruangdech , Nichada Boonsilp

1
1
1
Advisors: Benyaporn Patjareon , Orathai Lumsan , Prissana Wiriyajitsomboon
2
1
1

Princess Chulabhorn Science High School Lopburi
1
Kasetsart University
2

Abstract
Chopsticks are popular in use, especially for food in Asia. There are many types of chopsticks such as
metal chopsticks and bamboo chopsticks. Bamboo chopsticks are most used because they are cheaper
than the others. However, the survey found that these types of chopsticks tend to have fungus such as
Aspergillus flavus on them because of holes or flaws in the packages. Due to the excessive use of plastic
packages become one of the causes of global warming that affect both life and environment. Therefore,
we should manufacture biological packages instead of plastic packages for the reason that they can
inhibit this type of fungus and protect consumers and the environment from harm. There are 2 methods,
method 1 is galangal extract inhibition test and method 2 is forming biofilm package. There are 2 results,
result 1 is galangal extract at 100,000 ppm has the best result to inhibit Aspergillus flavus and result 2
is biofilm with galangal extract at 100,000 ppm are gauzy, thin, tough and slightly flexible. Our study
demonstrated that the galangal extract could inhibit this fungus. These results would be an initial step
to further study the impact of using the galangal extract on manufacturing sector.

Keywords: galangal, Aspergillus flavus, fungus, biofilm, chopsticks

























23

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



A Comparative study of the effectiveness of crude extracts from fungus
Beauveria bassiana Wild type strain, Overexpression pks14 FH strain,
Overexpression pks14 F30 strain as an inhibitor of amoeba cells


Dalad Rattanakrn , Chanakarn Chalearmmeung
1
1
Advisors: Bussarin Jitseng , Alongkorn Amnuaykanjanasin
1
2

Princess Chulabhorn Science High School Satun
1
National Science and Technology Development Agency
2



Abstract
The objective of this research was to compare the effectiveness of crude extract metabolites from
Beauveria bassiana fungus Wild type strain, Overexpression pks14 FH strain and Overexpression pks14
F30 strain as an inhibitor of amoeba cells. There were 2 experiments: 1) Culturing and extracting 3
strains of Beauveria bassiana by using a simple extraction and use Ethyl acetate as a solvent, 2)
Compare the effectiveness of crude extract as an inhibitor of amoeba cells. We divided the experiments
into five groups. In each group using 1 mL of amoeba cells mixed with crude extract from 3 strain of
Beauveria bassiana, 100 microliters for 1 strain. The experimental control group are the tube 1 and 2.
There are tube combination of amoeba cells, Methanol and PBS, respectively. The result revealed that
the mortality rate of amoeba cells in tube 4 and 5 with a mixture of amoeba cells, crude extract
(Overexpression pks14 F30) and crude extract (Overexpression pks14 FH) is 100%. Followed by the
third tube is the combination of amoeba cells and crude extract (Wild type) which has the mortality rate
of amoeba cells is 28.2%. We can conclude that crude extract metabolites from Overexpression pks14
FH and Overexpression pks14 F30 of Beauveria bassiana are more effective as an inhibitor amoeba
cells than crude extract metabolites from Wild type of Beauveria bassiana.


Keywords: Beauveria bassiana, Wild type strain, Overexpression pks14 FH strain, Overexpression
pks14 F30 strain



















24

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Development of Cold and Hot Gel Pack from Carboxymethyl Cellulose
by Durian Rind

Naphasakit Chaiyota , Pichamon Teabpeach , Apatsara Namsri
1
1
1
Advisors: Namthip Srikaew , Surasuk Boontima
1
1

Princess Chulabhron Science High School Loei
1


Abstract
The objective of this project is to develop a cold and hot medical gel from carboxymethyl cellulose
from durian rind. By studying the preparation of Hydrogel 3 properties as following. Hydrogel ACG,
Carboxymethyl cellulose, Alginate and Guagum as a substrate-binding with double-mechanical via
sodium tetraborate and calcium chloride. Studying the differentiation of substrate ration when the
binding substance is equal to 0.5 and 1.0 percent by volume. The production hydrogel will be tested in
absorption state by testing absorption in different acidity of water and different of temperature. Test the
change in temperature of gel and test the ability to maintain the temperature in the gel. Found out that,
a hydrogel with A1C3G6-10 formula has the highest ability to maintain the temperature. Therefore, the
A1C3G6-10 formula is the most appropriate formula for medical use. Because of the soft texture, high
flexibility, high capacity of absorption, and fast change in temperature.


Keywords: hydrogel, carboxymethyl cellulose, cold and hot gel pack, durian rind

























25

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



NanoFiber Band-Aid mixed with Rind Extract of False Daisy
Helps Blood Clotting faster


Naphat Jakkawong , Suchonkan Khotthakho
1
1
Advisor: Meenarat Wongsanae
2

Princess Chulabhorn Science High School Mukdahan
1
Ubonratchathani University
2




Abstract
The objective of the study is to study NanoFiber Band-Aid mixed with Rind Extract of False Daisy
Helps Blood Clotting faster the purpose of this study were: 1) to study the efficiency of the extract from
the mucous membrane to the hardening of the blood, 2) to measure Flavonoid quantity in Rind Extract
of False Daisy, 3) to study about the efficiency and longevity of the extract of False Daisy, 4) to study
the efficiency of NanoFiber Band-Aid obtained by the proceeding four steps. Firstly, make a
comparison between various concentration levels of Rind Extract of False Daisy affect the blood
clotting, the results appear that 4 %W/V makes the most efficient blood clotting. Secondly, finding the
Flavonoids dose by aluminium nitrate colorimetric method and using quercitin ,as a standard solution,
found that Flavonoids has a volume of 1,089±0.03 mg quercetin per 1g of the extract part 3 the study
of the formation of the nanofiber wound plate protection of the extract are found at a ratio of PVA :
CMC : starch : glycerol: False Daisy extract as 4:3:2:0.1:0.9 made by Electrospinning a
nanotechnology, using 12 kilovolts of electrical difference, the distance between needle and aluminium
foid as metal collector is 15 centimeters, and release rate of 1 milliliter of solution per hour,can be
absorbed at 11.604 as 10 time. You'll get Nanofibers made from composite materials were obtained and
the fourth study of the coagulation effect of the nanofiber wound dressing of Rind Extract of False
Daisy was found to have hemostatic properties and accelerate the Coagulation of the
blood.

Keyword: Rind Extract of False Daisy, Blood Clotting, Electrospinning, Nanofibers, Flavonoid

















26

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Study of the effects of heavy metals on growth and comparison of
heavy metal adsorption efficiency of Large duckweed
(Spirodela polyrrhiza (L.) Schleid.)


Pachara Chaipinitnorachart , Tepprecha Teebunma
1
1
Advisors: Panupong Kampanit , Phakpoom Phraprasert
1
2

Princess Chulabhorn Science High School Chonburi
1
Burapha University
2



Abstract
The objective of this research was to compare the efficiency of heavy metal adsorption and study the
effect of heavy metals on the growth of duckweed (Spirodela polyrrhiza (L.) Schleid.) by using Large
duckweed from the dormitory pool at Princess Chulpabhorn Science High School Chonburi. The
experiment was divided into 2 steps. The first step was to study the adsorption efficiency of heavy
metals (Copper: Cu) of Large duckweed. The experiment was carried out by using 50 Large duckweed
/ experiment set in the copper (II) sulfate (CuSO4) solution at concentrations of 0.2M, 0.4M, 0.6M, 0.8M
and 1M, data were collected for 1, 3, 5 and 7 days. The experimental results of copper metal
continuously decreased. The adsorption of copper metal of Large duckweed was the most effective
adsorption at the concentration of 1M which could absorb copper by 0.00027%. The second step, study
the effects of heavy metals on the growth of Large duckweed from measuring the root length, the
average root length of Large duckweed increased and the average root length correlated with the
concentration of copper (II) sulfate solution over time. Including the determination of chlorophyll, A
and chlorophyll B by extracting with acetone. After that, the absorbance was measured and the amount
of chlorophyll A and chlorophyll B were increased after the experiment was completed. Research has
shown that Large duckweed are effective in absorbing heavy metals. And with a growing rate of growth
Therefore can be used as a prototype plant to absorb heavy metals in the environment.

Keywords: Large Duckweed, CuSO4, Heavy metal



















27

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Study of the Oral Bacterial Inhibition of Crude Edo Longan Seed
(Dimocarpus longan) Extract


Apirat Wijarn , Ekkapong Jomwong , Kitisak Sawangphanapan
1
1
1
Advisor: Anek Chaiboot
1

Princess Chulabhorn Science High School Chiang Rai
1



Abstract
The study aimed to examine the oral Bacterial inhibition of crude extract from Edo longan seed.
The experimental method had 2 steps. The first step was to prepare crude extract from longan
seed by using water as solvent. Then the solvent was evaporated by Rotary Evaporator. For the
preparation of bacteria, 1 ml of oral secretions was put in Nutrient Broth food in test tubes and
was incubated for 24 hours. The second step was to test the turbidity of the samples that
contained oral secretions from test tubes after 24,48and 72 hours incubation. The bacteria,
prepared in the test tube, were added by crude longan seed extract at the concentration of 0.1
g/ml in the amount of 0.1 ml - 1 ml. After incubation for 24 hours, the experiment was found
that the turbidity of the samples were decreased. Thus, the crude longan seed extract could
inhibit the growth of oral bacteria. The amount of crude longan seed extract at 0.8 ml showed
the highest inhibitory growth ability in oral bacteria with the average turbidity of 55.27 NTU
and the turbidity range of 73.80 – 55.27 NTU. In conclusion, the crude longan extract has
bacterial inhibitory ability in oral bacteria from chemical components such as polyphenol
compounds.


Keywords: Edo Longan, Bacterial inhibition, Polyphenol compounds





















28

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Preparation and Study of Efficacy of Beads Mixed with Fang Herbal
Extract for Medical Application


Benjapohn Khamthapthim , Phithaiporn Saesue
1
1
Advisors: Natpassorn Laonet , Krit Sutjarittangtham ,
1
2
Wipharat Chuachuad Chaiyasith , Worawut Kriangkrai
3
3

Princess Chulabhorn Science High School Phitsanulok
1
Pibulsongkram Rajabhat University
2
Naresuan University
3

Abstract
The objective of the present project was to study the suitable type and amount of polymer mixed with
herbal extract to forming beads that can inhibit the growth of bacteria. First, the effect of bacteria
inhibition of Fang herbal extract was studied. It was found that the extract can inhibit bacteria and had
a MIC value of 3.125 mg/ml. Second, the ratio of soluble polymer and Fang herbal extract in bead
forming for bacteria inhibition of Staphylococcus aureus was investigated. The results showed that the
efficacy of beads with different polymer ratios gave different abilities to store herbal extract. The beads
containing a polymer at a ratio of 40 A: 60 W have the most effective storage of extracts. As for the
study of emulsion stability, it was found that polymer at a ratio of 80 A:20 W and 100 A:0 W gave the
separation of layers. From the study of the properties of the beads at the ratio 40 A: 60 W using Fang
extract at the concentration equal to MIC and for the retention of extract study, it was found that the
amount of Brazilin 0.00189 mg/ml by bead during 0-120 minutes will gradually release but for the 120-
150 minutes, there are large amounts of extracts released. From the study of bead swelling, it was found
that the beads had small swelling in the period of 0 - 15 minutes and there was no further swelling. For
a study of a physical characteristics, it was observed that the beads were spherical in shape, smooth
surface, with an average diameter of 1.75 mm. When the inhibitory activity was tested, it was indicated
that the bead mixed with herbal extract at the concentration equal 6MIC gave the most effective
inhibition of infection with the diameter of the average inhibitory area of 2.18 cm. Finally, to develop
an initial product containing beads from polymer encapsulated herbal Fang extracts for medical uses,
when cream was mixed, the suitable formula ratio of cream: bead was 5:1 with the diameter of the
average inhibitory zone of 2.09 mm. It was also found that when shaking the cream, it will increase the
releasing efficiency of the extract from beads.


Keywords: Fang herbal extract, beads, Polymer, Cream mixed with beads














29

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Study the efficacy of Myrtaceae family’s extraction on the Streptococcus
mutans inhibition for teeth decay prevention and Study of adhesion and

detachment of extracts compare with carbonated water
and anticaries agents at the enamel layer of crown and root
for the enamel coating development



Kittinut Banchajarurat , Tanapat Buranaprasertsuk
1
1
Advisors: Pornpan Chomwong , Warangkhana Thuphakde ,
1
1
Pagakrong wanapaisa , Rangsima Sakoolnamarka
3
2

Princess Chulabhorn Science High School Pathumthani
1
Mahidol university
2
Chulalongkorn University
3

Abstract
In this study, the efficacy of enamel from Myrtaceae family’s: apple, guava and jambul, compared with
chlorhexidine. To extract the crude extract in each plant, the extract was obtained for oral antibacterial
testing by 95% ethanol extraction, filtered and dried under vacuum condition. And test for inhibition of
Streptococcus mutans infection causing caries decayed tooth by disc diffusion on Mueller Hinton agar
at initial concentrations of 1: 2, 1:10, 1: 100 and 1: 1000 over 12, 24 and 48 hours, repeated. 6 times to
find the mean and standard error. It was found that at the initial concentration, chlorhexidine effective
in inhibiting Streptococcus mutans bacteria was the most followed by the guava from the antibacterial
study and rose apple respectively. From the study of inhibition on Streptococcus mutans was found that
Myrtaceae family crude extract had maximun ability to prohibit Streptococcus mutans at 12 h.
Chlorhexidine had the highest inhibitory efficacy at 48 hours and guava extract can inhibit bacteria. The
most common Streptococcus mutans was with 60% inhibition percentage, followed by rose apple with
50% inhibition percentage and jambul was unable to inhibit these bacteria. In part of the dental adhesion
and detachment experiment, the teeth were soaked in extracts from the Myrtaceae family, chlorhexidine,
fluoride and carbonated water coatings to test the adhesion of substances then the teeth that were soaked
in carbonated water were further soaked in various extracts. To test the detachment and adhesion of the
extract compared with chlorhexidine, then it was further developed into tooth enamel.


Keywords: Myrtaceae family, Decayed tooth, Streptococcus mutans, Chlorhexidine, Disc diffusion












30

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Characterization of volatile compounds improving the hydroponic
technique for strawberry


Kensuke Goto , Shuhei Ichiraku
1
1
Advisor: Izumi Sasanuma
1

Oyama College
1




Abstract
Plant diseases are a major cause for yield losses and new strategies to control them without harming the
environment are urgently needed. Companion planting contributes to the plant health in diverse ways,
among which the emission of disease-inhibiting volatile organic compounds (VOCs). Here we report
VOCs emitted by companion plants can be used as chemical signals providing better quality for
strawberries during the hydroponic culture. In a separate paper, we reported that the VOCs of
companion plants caused strong growth stimulation of the strawberries. This work focuses on VOCs
released from companion plants and demonstrates the high in plant protective potential of VOCs, which
prevented gray mold disease in strawberries without phytotoxic effects, in contrast to other fungicides.
This work brings new perspectives for plant protection against the devastating pathogen of strawberries.


Keywords: volatile organic compounds, companion planting, strawberry, β-glucosidase



























31

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Synthesis and Applications of Carboxymethyl cellulose water hyacinth
and bagasse for extend durability and protect agricultural products after
harvest of rice


Puwadet Ketkaew , Pisith Noinu
1
1
Advisor: Sukonthip Kaewla-ead
1

Saparachinee Trang school
1




Abstract
This project has purposes to synthesize Carboxymethyl cellulose from water hyacinth and bagasse, to
study ability in protection against mold and bacteria by using CMC from water hyacinth and bagasse to
coat the rice, and to study the germination index of rice coated with CMC from water hyacinth and
bagasse by the experiment that keep rice with no coating and rice coated with CMC from water hyacinth
and bagasse at 5 degree Celsius and 95 percent humidity for 1 month then check for quantity of mold
and bacteria with spread plate method. The measure of growth of mold and bacteria found that ability
in protection against mold and bacteria, rice without CMC coating had 4.56x103CFU/g of mold
9.0x103CFU/g of bacteria which are greatest, following with rice coated with CMC from water hyacinth
had 5.5x102CFU/g of mold 2.90x103CFU/g of bacteria, and rice coated with CMC from bagasse had
5.0x102CFU/g of mold1.5x103CFU/g of bacteria which are the least respectively. The germination
index measure experiment of rice that coated with Carboxymethyl cellulose from water hyacinth and
bagasse and rice without coating found that there was no significant difference. Consequently,
Carboxymethyl cellulose extracted from water hyacinth and bagasse was able to extend durability and
protect postharvest product of rice.


Keywords: Carboxymethyl cellulose




















32

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Wastewater Treatment


Kanokkan Thanombun , Sirimat Photisan
1
1
Advisors: Juree Chunban , Kittichon U-taynapun
1
2

Cha uat School
1
Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat Campus
2



Abstract
This project was developed from the last version of our automatic fish feeder, because each time the
fish feed there will be sediment from the fish feed and fish residue in the pond, causing high ammonia
and organic matter in the fish pond. As a result, fish farmer has to change water and use a lot of water,
so our group has an idea to use waste water from fish farming. To make it more useful by creating a
wastewater treatment system based on knowledge of the nitrogen cycle in order to make the water
suitable for use in fish farming. In order to increase the production in the field of agriculture and to
make the wastewater from the fish tank to be able to use more from the project of wastewater treatment
system which corresponds. According to the objectives laid down in order to build a wastewater
treatment system based on the results of the comparison between to 1 with the water treatment system
with oxygen in the water, ammonia, nitrite and nitrate values suitable for farming. Fish more than the
second tank and found that the water in the 1st tank that has color water treatment system, no water, no
residue, can use the water that has been treated to grow vegetables and Each fish can be recycled again
the efficiency of wastewater treatment equipment is working efficiently.


Keywords: fish farming, wastewater






















33

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



A humidity control system in an automatic mushroom house


Tanapon Onsubsai , Piyaporn Jansong
1
1
Advisor: Khwuanchira Chamnankul
1

Yangrak Wittaya School
1




Abstract
Checking and controlling environment in mushroom house is very important because it has an effect
for growing mushroom. If in the mushroom house has a suitably humidity it makes the mushroom
outgrow that we want. The purpose of this project is to increase product and high quality of mushroom
by developing a humidity control system that suitable for people who grow mushrooms. We control
water by using an automatic material with checking humidity in mushroom house. There is a sigh
electronic send to humidity central control for compare relative humidity that we set at the first time. If
the relative humidity is not equal of the first time, the control system will send the sign to water pump
system. After that the water pump system will send water pass water pipe and springer that design it to
spread water around the mushroom house. And the left water from using it we keep it in the clarifier
passing the strainer and we can use it again. Therefore, the agriculturist can reduce equipment cost, save
water and save labor watering the mushroom too.


Keywords: Mushroom house, humidity

























34

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Study of Antibacterial in Chili Pepper and Pepper Extract


Nongnapas Hengpatomwat , Panyawee Phuimart , Suphitcha Komonsai
1
1
1
Advisors: Jansuda Saengsoda , Jesada Karnchanajindanun , Natenapha Kledchine
2
1
1

Loeipittayakom School
1
Loei Rajabhat University
2

Abstract
The project's purpose is to study and to compare the extracted of pepper and chili pepper. The bacteria
that were used are Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Enterobacter
aerogenes and Pseudomonas aeruginosa. The extracts of chili peper, peper, bacteria on the nutrient
agar and 70% Ethanol were cultivated into peper disc. Incubate the mixture in the incubator to evaporate
the solvent. Put the mixture (chili pepper, pepper, 70% Ethanol) on top of each bacteria that was on top
of nutrient agar. Incubated for 18 - 24 hours, then measure the inhibition zone or clear zone of each
paper disc on each bacterium. The goals of methodology were as to see if the pepper extracted can
inhibit Escherichia coli (Clear zone: 1 cm) and Pseudomonas aeruginosa (Clear zone: 1 cm). The chili
pepper can inhibit only Pseudomonas aeruginosa (Clear zone: 0.8 cm) as same as the control disc.


Keywords: Extracted, chili pepper, pepper, Staphylococcus aureus, Escherichia coli, Klebsiella
pneumonia, Enterobacter aerogenes and Pseudomonas aeruginosa

























35

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Growth Inhibitory Activities of Crude Extracts Obtained from Local
Plants against Escherichia coli


Thanachot Boonmak , Satawat Kerdtin , Wiwat Upakarneekit
1
1
1
Advisors: Nutthaya Konklong , Supa Singtookreaw
1
1

Watjuntrawas (Sukprasarnrat) School
1


Abstract
This study was a test to compare the activity against Escherichia coli causing diarrhea or food poisoning
by using coarse extracts in ethanol from 5 local plants in the ratio of 1: 10 grams per volume at room
temperature. From the paper disc diffusion inhibition test, it was found that the crude extract from
Wildbetal Leafbush (piper sarmentosum) had the best inhibitory effect, followed by the lemongrass
(Cymbopogon citratus) with the clear diameter of the inhibitory zone. (Inhibition zone) equals 18.69 ±
1.2 and 11.22 ± 2.59 mm, respectively. And no inhibitory effect was found in Water hyacinth
(Eichhornia crassipes), Guava (Psidium guajava) and Kariyat (Andrographis paniculata) when
considering the efficacy of crude extract from Wildbetal Leafbush to inhibit the growth of Escherichia
coli bacteria. It was found that the efficiency of the extract increased with the clear zone, the diameter
of the clear zone increased from 10.89 mm to 18.69 mm. When increasing the concentration of 80% to
100%. In addition, the team developed a program to control automatic spraying devices from the
Kidbright board to stop the growth of Escherichia coli bacteria in the bathroom by creating a bathroom
model with the installation of a spraying device. And inquire about the opinions of users in 3 areas
which are physical, usage and safety Found that the satisfaction was at a high level with an average of
4.34

Keywords: Crude extract in the ethanol layer, Escherichia coli, paper disc diffusion, Kidbright




















36

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



A study of the effectiveness of the oil blotting sheet from sacred lotus stem


Thanaphorn Wongwattana , Warinthira Jarukarnm , Wuthipong Thepchang
1
1
1
Advisors: Atchariya Parapim , Kesorn Chaiyapet
1
1

Mukdahan school
1

Abstract
The purpose of this experimental science project is to study the production method of oil blotting sheet
from sacred lotus stem, to study the appropriate ratio for forming oil blotting sheet from sacred lotus
stem, and to study the efficiency of oil blotting sheet from sacred lotus stem by comparing the ratio
between the ratios to proper. The statistical average was applied for data analysis. The results showed
as follows. 1. Each ratio of the oil blotting sheets from the sacred lotus stem was appropriate for forming.
2. The oil blotting sheets from sacred lotus stem was able to absorb oil on the face. By the oil blotting
sheet from the 4th ratio was CMC: Tissue pulp: Glycerol: PEG 6000: Talcum: Chitosan solution were
3:2:2:2:3:100 (g: g: ml: g: g: ml) respectively which had an average tensile strength of 16.21 Newton,
an average oil absorption capacity was 1.78 square centimeters, average standard weight was 3.90 g,
the mean pH was 7.2 and there were no samples irritated by the use.

Keywords: Oil blotting sheet, Sacred lotus stem



























37

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Biomaterials based on Alginate and Polyvinylalcohol for Wound Healing
in the Laboratory


Baibua Borkird , Montraluk Prommanee
1
1
Advisors: Adinan Jehsu , Jirat Meesane , Jutakan Thonglam , Yadanar Mya Moe
2
1
2
2

PSU Wittayanusorn School
1
Prince of Songkla University
2

Abstract
In present day, synthetic biomedical material is used for tissue engineering, especially in the wound
healing. The commercial membrane becomes an alternative way, and it has a high ability for new tissue
formation. However, this membrane has high cost for patient implantation. In this study choose alginate
(complex carbohydrate is extracted from brown seaweed) that is common and cheap. The research of
Burapha University and Silpakorn University, Applications of Polymers from Marine Resource, shows
that alginate is able to inhibit inflammation and bacteriostatic action. Polyvinyl alcohol usually is used
for welding substance to be dense. Alginate and Polyvinyl alcohol (PVA) are polymers that can
fabricate into membrane form. Moreover, the biofunctionality of these polymers support for cell
migration and proliferation. In this study, the 5% by mass per unit volume of alginate and PVA is
combined at different ratio including 100:0, 70:30, 50:50, 30:70 and 0:100 3 ml per piece in square
mold that is examined 15 pieces each ratio. The freeze thawing method at -80 degree celsius is selected
for membrane fabrication and Freeze dry is conserved qualification of membrane. Calcium Chloride
was used for soaking the membrane in the last process to improve the mechanical property of the
membrane. This productive process is tested for following cell growth. Photomicroghaphy can be
recorded from using confocal microscope. Finally, overall are utilized to prepare the most effective for
cell growth that is a principal part of wound healing. The 70:30 of Alginate/PVA showed the good
performance for wound healing application. The concentration of solution results in forming. The first
experiment, which is mixed alginate solution with a concentration of 1%w/v and the PVA solution is
concentrated. The mass to volume ratio, which defines the process leading to a freeze thow result is a
mixture of such a change is a gel but has not been used enough in a gel adhesive.


Keywords: Fibroblast, Polyvinylalcohol, Alginate















38