Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Development of Molecular Markers to Identify Thai Herbs at
Species Level Authentication


Kornvarun Tirananmongkol , Atsuki Matsuzawa , Mingkwan Rattanasiwamok
1
1
1
Advisors: Supanan Sucharit , Pimpen Tiansittipong
1
1

Mahidol Wittayanusorn School
1


Abstract
Using appropriate species of herbs is essential for producing effective herb products because some
active ingredients can be found only in specific species. But identifying species by morphological
characteristics requires expertise and can be confusing. We decided to find a more precise way to
identify herb species by analyzing DNA sequences to find species specific sites. In this study, we chose
4 species of herb which are Stevia rebaudiana, Carthamus tinctorius, Mentha spicata and Gynostemma
pentaphyllum as our species of interest. We extracted DNA from plant tissues (leaves), performed PCR
amplification of ITS2 region, sequenced the PCR product, and analyzed the sequence by comparing
with closely related species in the same genus. For the result, we found 7 to 9 sites in
S. rebaudiana, C. tinctorius and M. spicata that have 70% or higher specificity. But in contrast, in
G. pentaphyllum we only found 3 sites with specificity of 65.22%. We concluded that ITS2 region can
be used to specify S. rebaudiana, C. tinctorius and M. spicata, but might not be suitable for specifying
G. pentaphyllum.

Keywords: DNA sequences analyzing, Molecular marker, ITS2 region, Thai herb






















39

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The study of the efficiency of the absorption of the essential oil on plants
extended in automatic aromatherapy lamp


Jularat Singkaew , and Techit Jabplang
1
1
Advisors: Khwanjai Karnchanasrimek and Anis Manmanah
2
3

Wichienmatu School
1



Abstract
This project aims 1) to study the absorption efficiency of extracted oil from cellulose in different type
of plants. 2) to study the efficiency of extracted oil from cellulose in automatic aromatherapy
lamp. Firstly, extract the cellulose fibers from corn silk, coconut fiber, and pineapple fiber. Secondly
bleach the cellulose fibers with sodium hydroxide and hydrogen peroxide. Thirdly, dry the fibers in the
sun. Lastly, test the absorption efficiency of 3 types of oil which was extracted from corn silk, coconut
fiber, and pineapple fiber. The efficiency of those 3 types of oil is 61.53%, 70.58% and 75%
respectively. The cellulose sheet that absorbs the most essential oils were pineapple fiber. The cellulose
will be put in the lamp the has ESP8266 command broad kit which can enable/disable the emission of
the oil via Blynk mobile application. The sensors will be installed for measuring the moisture of
cellulose sheets. When the humidity level reaches the preset value, the command board kit will
automatically stop the aromatherapy pump. The cellulose pad will be diffused the soothing aroma of
the essential oils by pressing the command button in the mobile application.

Keywords: Cellulose, Automatic aromatherapy lamp, Absorption
























40

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Efficiency of concrete materials made with waste foundry sand
treated with fungi


Nobtham Jirajarus , Phutanate Pisutsin , Sarunwich Juntramin
1
1
1
Advisor: Pattaraya Klintong
1

Mahidol Wittayanusorn School
1



Abstract
Nowadays, solid waste is the critical problem in the world. Waste foundry sand (WFS) is one of the
most common waste generated by metal casting industries. Due to high cost and lack of land filling
space, Waste foundry sand is one such material which can be used as an alternative to natural sand in
concrete. This study aimed to evaluate the effects of fungal treated WFS on concrete properties. A total
of 10 fungi were isolate from area of Mahidol Wittayanusorn School. They were analyzed for acid
production. The result showed that isolate I9 was the best acid producer. The identification results found
that isolate I9 was belonged to genus Aspergillus by colony and morphological features. Fungal
inoculums (2%) were used for the treatment of WFS for 10 days. Fungal treated WFS and untreated
WFS were utilized for the production of concrete cubes (15x15x15 cm) mixtures with a substitution
rate of 0,10 and 20% by volume. The efficiency of concrete was evaluated through compressive strength
property. The test results showed that the compressive strength of the concrete made with fungal treated
and untreated WFS (10% and 20%) was relatively equal to the control concrete. Therefore, WFS with
a substitution rate of 20% can be effectively used as partial replacement of sand in concrete production,
however, concrete containing fungal (Aspergillus sp.) treated WFS (10% and 20%) didn’t show the
increase in compressive strength as compared to concrete made with untreated WFS. From the test
results, it is suggested that further studies are necessary to seek other appropriate fungal strains and
suitable conditions treatment for waste foundry sand.

Keywords: Waste foundry sand, Fungi, Concrete


















41

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Cellulose-Chitosan Absorbents for Water Treatment

Jiratchaya Karavapong , Sirabhadra Naraballobh
1
1
Advisor: Thanasan Nilsu
1

Kamnoetvidya Science Academy
1




Abstract
Heavy metals in water have become more concerned as a major environmental issue due to its hazardous
effect on humans and other organisms. Cellulose and chitosan biopolymers were chosen as heavy metal
absorbents considering their high potential in decontaminating heavy metal ions in wastewater. A
cellulose-chitosan hydrogel composite was prepared as a biodegradable bio-absorbent through structure
modification and cross-linking. To enhance ion absorbability, the functional groups in cellulose and
chitosan polymers were chemically modified before modeled as hydrogel beads in cross-linking
process. The hydrogel was formed by ionotropic gelation of alginate in CaCl2 solution. In hydrogel
formation, alginate hydrogels from cellulose, carboxymethylated chitosan, and a combination of
cellulose and carboxymethylated chitosan were prepared successfully. Then, the cross-linked hydrogels
were tested for their water and salt retention capability to evaluate salt absorbability. As a result, the
cellulose hydrogel performed the highest ion absorbability, followed by cellulose and
carboxymethylated chitosan hydrogels. Moreover, the heavy metal absorption was carried out with
CuCl2 aqueous solution in various pH conditions (pH 2-6) to represent wastewater. The concentration
of Cu was measured using a colorimetric method. The results revealed that, at pH 4, every type of
2+
hydrogels showed the highest absorption of Cu . In addition, hydrogel beads from carboxymethylated
2+
chitosan and a combination of cellulose and carboxymethylated chitosan could absorb Cu more
2+
efficiently than cellulose hydrogels. The absorption mechanism would be further investigated using
scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR). These
experiments can be concluded that hydrogel beads from modified biopolymers could potentially absorb
heavy metal ions in wastewater.

Keywords: Cellulose, Chitosan, Heavy metal ion, Hydrogel, Cross-linking


















42

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Super Water Absorbent Foam from Rubber Crystalline
Bacterial Cellulose


Kewalin Sukkwai , Chariya Noonoi
1
1
Advisors: Suwaree Pongtheerawan , Chalermporn Pongtheerawan
1
1

Suratpittaya School
1



Abstract
Super Water Absorbent Foam from Rubber Crystalline Bacterial Cellulose was created for solve the
problem of soil water shortage and reduce water in agriculture. Our inspiration came from the observe
of the Sponge foam from rubber, that has a lot of porous. It can absorbed and retains the water well
same as Hydrogel. So, we interesting in Gelatinous bacterial cellulose because of it can 60 -700 precents
water retention. it can growth in waste water from old coconut juice and wastewater from fruit peel. In
Gelatinous bacterial cellulose has high Hydrogel and has more Alpha cellulose than plant cellulose. So,
we modify the structure of Gelatinous Bacterial Cellulose in to Crystalline by Alkaline degradation and
Acid hydrolysis. After that we make the nature foam by mixed the Crystalline with the Para Rubber. In
this study, we found that the optimum ratio for make the smaller porous from rubber foam was using
para rubber crystalline bacterial cellulose ratio 25:0.5 by mass. After that we test the Mechanical
properties, Physical properties and Biological properties test. We found that the rubber Crystalline
Bacterial have 39.5 µm size porous average, Small porous average 28.02 um, big porous average 61.66
um, Small porous can absorbs water by capillary action. Meanwhile, Super Water Absorbent Foam
from Rubber Crystalline Bacterial Cellulose can be retention water well. Because of the properties of
crystalline cellulose has a lot of surface area and a lot of hydroxyl groups that can form hydrogen bonds,
So It well for absorb and retain water. The property of this invention were 380 percents absorption, The
cool of this inventions were super absorbent polymers (SAPs). Its biodegradation in 90 days in soil. It
can reduce water for plantation. it has low price because in bacterial cellulose culture it used less time,
less space and high cellulose product than other plants above all it more Crystalline Cellulose 92-100
% higher than other raw materials and higher than all plants. we can reduce water for 4 days in mung
bean plantation, at relative humidity 65-75 percent. SAPs had cost 0.05 baht/cm. So this invention is
the best for save the water in planting Because of agriculture as the most important job in the world
wide.

Keywords: Water absorbent foam, Crystalline cellulose













43

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



A Study about an Efficient and Low Cost Closed Recirculating System
(For the Future of Aquaculture)


Misawa Haruka , Kato Misaki , Hirata Saeko , Otaki Hiroko
1
1
1
1
Advisors: Osawa Ryo , Ushizawa Honoka
1
1

Fukushima Prefectural Fukushima High School
1

Abstract
The Great East Japan Earthquake occurred in 2011. The marine products industry in Fukushima was
badly damaged by the Fukushima nuclear power plant accident. Therefore, we have focused on a closed
recirculating system. This type of farming enables us to farm fish on land. We have studied it from the
viewpoints of filtration, water and food. It has been successful. However, if we applied all these results
for the aquaculture system, it would cost a lot. Especially, conventional filter material is expensive.
Then we thought that we should use another filter material to reduce the cost. This is now under
evaluation. We will report this result in the full paper. Also, from February 2020, we are constructing a
new aquaculture system, which is efficient and low cost. From April, we will start farming mainly eels
with the system that we make. The initial cost of our system on a 15 ton tank scale is 6 million yen,
while the initial cost of aquaculture systems used by ordinary companies on a 15 ton tank scale is 7
million yen. Comparing our system with the ordinary system, our system could be said to be a low-
cost-system. From the year 2021, we would start providing our system to people who want to start fish
farming as a business with our system. In this way, we would like to boost the marine products industry
in Japan, and spread this aquaculture system to the world.


Keywords: aquaculture, closed recirculating system, low cost, fish, filter material





















44

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Steps toward Making Artificial Culture of Plant Worms Efficient


Kosuke Yamada , Rikito Yamatsu
1
1
Advisor: Teruyoshi Yamaguchi
1

Meijo University Senior High School
1




Abstract
There are some illnesses that cannot be healed by medicine. Plant worms have unlimited potential to
heal illnesses that cannot be cured by conventional medicine. Plant worms have been used as herbal
medicine since ancient times and it is possible for the development of new medicine, but a stable supply
would need to be made by artificial cultures. There are two types; anamorphs and teleomorphs, the latter
is formed by synthesizing two spores of anamorphs. Also, previous research suggests that teleomorphs
can be divided into anamorphs, which means we could develop a way to stabilize and sufficiently obtain
them.


Keywords: plant worms, teleomorphs, anamorphs




























45

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Using Low Allergenization of Wheat by Aspergillus


Nozomi Umeda , Mizuki Watanabe
1
1
Advisor: Teruyoshi Yamaguchi
1

Meijo University Senior High School
1




Abstract
In Japan, disasters often happen such as heavy rain, typhoons, and earthquakes. Emergency food which
is provided for disaster victims contains a lot of contents of wheat. It is well known for causing serious
problems to patients with wheat allergies. Previous research suggests that aspergillus could decompose
wheat gluten because of an enzyme in it. So, we researched whether aspergillus could decompose wheat
globulin and wheat albumin.

Keywords: aspergillus, low allergenization, wheat allergies
































46

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Difference between Microbial Fuel Cell and Other Renewable Energy


Yuki Oba , Yusei Ito , Futo Yamazaki
1
1
1
Advisor: Teruyoshi Yamaguchi
1

Meijo University Senior High School
1



Abstract
In recent years, energy problems are one of the many serious global issues; the method of generating
power by the use of renewable energy, particularly microbial fuel cell has been in the limelight. One of
the biggest advantages using the microbial fuel cell is that it can generate electricity anywhere by merely
using only an organic solution, though the electric power is minuscule. So, we conducted an experiment
in order to raise the electric power. Our method of the microbial fuel cell is that it should be laid on an
organic solution that can alter the mud of a river, which in turn causes electric power to rise. Our goal
is to raise electricity power strong enough to start an engine.


Keywords: microbial fuel cell, renewable energy, organic solution






























47

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Extermination of Aphids by Allelopathy


Hinano Yamagishiand , Nodoka Nishikawa , Noa Sagawa
1
1
1
Advisors: Teruyoshi Yamaguchi , Yasuhiro Yoshikawa
1
1

Meijo University Senior High School
1


Abstract
Food short age is a global issue around the world, which is particularly caused by harmful insects
damaging crops. A lot of damage is caused by aphids, leading to respiratory infection and other diseases.
Aphids are parasitic on plants which can be seen throughout the year. We researched on how to decrease
damage caused by aphids. Also, we used allelopathy effects to exterminate aphids. Plants that have
allelopathy effects can exterminate harmful insects. So, we studied which plants have the most
allelopathy effects. Plants that have allelopathy are marigold, mint, Chinese chive and rosemary.

Keywords: allelopathy, food shortage, aphid
































48

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Effects of Blue Light and UV-A Give Plants


Iori Oie , Inori Oie
1
1
Advisor: Yoriko Ikuta
1

Nara Prefectural Seisho Junior and Senior High School
1




Abstract
According to previous research, irradiation of plants with UV-B or UV-A cause’s growth inhibition.
Specifically, previous studies made use of radish sprouts which were irradiated with Blue and UV-A
light, had significantly longer stems and larger amounts of polyphenol than radish sprouts which were
irradiated with Blue light alone. In addition, researchers were able to confirm the presence of a
polyphenol which absorbed the UV wavelength. We hypothesized that anthocyanin was generated as a
defensive substance when irradiation with Blue Light and UV-A occurred, and that stomatal opening is
promoted by Blue light. In conclusion, this study confirms the existence of a substance which absorbs
short wavelengths when irradiated with Blue or Blue and UV-A light. Finally, it was shown that there
was no substance generated when irradiated with Blue and UV-A light. Furthermore, the current study
illustrated that it is not possible to use a spectrophotometer to distinguish between light absorbing
substances in radish sprouts using a spectrophotometer.


Keywords: irradiation of plants, UV-B or UV-A, Blue light, a spectrophotometer, radish sprouts


























49

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Inhibition of active behavior of stink bugs


Ichiro Machida , Rina Yamanaka ,Hidenori Nakano , Yositeru Watanabe
1
1
1
1
Advisors: Takayuki Fukuda , Rina Iwaki
1
1

Ritsumeikan Keisho High School
1

Abstract
Our research theme is the inhibition of active behavior of stink bugs. At Ritsumeikan Keisho High
School, to which we belong, by stink bug infestations occur every summer and autumn. We thought
students could possible to remedy the problems caused by stink bugs. Therefore, the research theme
"Inhibition of active behavior of stink bugs" was chosen. Insects display phototaxis behavior, movement
in response to ultraviolet rays emitted from light sources. Stink bugs also have this behavior. In addition,
some light sources emit ultraviolet rays and others do not. For example, light sources such as the sun,
moon, and fluorescent lamps emit ultraviolet rays, but light sources such as LEDs do not. All the light
sources used in our school buildings are fluorescent lights. One of the causes of stink bugs invading
may be the ultraviolet rays emitted from the fluorescent lamps inside the school. It is suspected that
stink bugs gather because of the ultraviolet rays emitted from light sources near the windows of our
school. If stink bugs are confirmed to be repelled by LEDs, the long-standing damage caused by stink
bugs would be resolved. Therefore, we will focus on the phototaxis of stink bugs and clarify how
invasions of stink bugs change depending on the light sources in the school buildings. We will prepare
a bug cage and partition it with a wall with enough holes for stink bugs to readily pass through. One of
the two spaces created will be considered "inside the school building", and the other "outside the school
building". Fluorescent lamps or LEDs will be installed "inside the school building". Stink bugs will be
released "outside the school building” adequate food and water. We will observe the movement of the
stink bugs and confirm what kinds of behaviors they exhibit. We also will compare the behavior of stink
bugs when either fluorescent lights or LEDs are installed in the school building to see how the light
sources in the school buildings affect the behavior of stink bugs.

Keywords: stink bug, active behavior


















50

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Changes in plant respiratory volume due to sound


Oyama Shota
1
Advisor: Takayuki Fukuda
1

Ritsumeikan Keisho High School
1




Abstract
The purpose of my research is to see if plants perceive sounds and change their respiratory volume.
Sound has various effects, such as stimulating people to increase their concentration, but some reports
have claimed that it might also affect plants besides humans. In 1968, Dorothy Ria Lak reported that
classical and rock music influence plant growth. According to a research report conducted by Nihon
University on the theme of "the effect of sound on plants," changes in glucose metabolism of plants
were affected by sound, but details of respiratory volume were not reported. So, I wanted to find out if
there was a relationship between breathing volume and sound. The experimental method is to cover the
plant with cardboard to darken it and induce breathing. After waiting for a certain period of time, let
the plant perceive the sound frequency and measure whether there is a change in the amount of carbon
dioxide using an air quality monitor. If this experiment is successful, we can see that sound changes the
respiration rate of plants. Based on the above, my research theme is the change in plant respiratory
volume due to sound.

Keywords : plant respiration, frequency perception


























51

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



What do jell coats have that Steinberg’s solution does not?


Akari Kageoka , Yuri Yasuda
1
1
Advisor: Genkuro Hashioka
1

Notre Dame Seishin Gakuen Seishin Girls’ High School
1




Abstract
We have been researching the differences between Steinberg’s solution (100%) and the jell coats which
protect the eggs of fire belly newts. Steinberg solution (100%), which keeps the same osmotic pressure,
is what we use when we remove the jell coats from eggs. However, in previous research almost all the
eggs put in Steinberg’s solution died, though they did not in jell coats. We thought there was a problem
with Steinberg solution. We hypothesized jell coats have something that Steinberg’s solution does not.
We decided to find out what that was. After we obtained newts’ eggs, we divided them into two groups:
one group for jell coats and the other one for Steinberg’s solution. We observed them for eight days but
only few of them developed and all of the others died. We have discussed why it happened and what
we can do for next. The spawning season is over now so we are trying to do something that does not
require many eggs.

Keywords: Steinberg’s solution, jell coats, osmotic pressure



























52

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The research in the method of reducing stress: A relationship between
classical songs composed by Mozart and grown-up broccoli sprouts


Moeka Nozawa
1

Incorporated School Ichikawa Gakuen Ichikawa Junior & Senior High School
1




Abstract
I researched the influence when I gave the broccoli sprouts Mozart’s music. There are three different
patterns under the conditions of the broccoli sprouts. Firstly, I had the broccoli sprouts hear Piano
Sonata No.11 in A major K.331 (Mozart), which I had played. Secondly, I had the broccoli sprouts hear
reverse reproduction of the music. However, thirdly, I had broccoli sprouts hear no music. My theory
is that the fastest pattern would be these of the first, and the second one would be the third, and the third
one would be the second. I wonder why the second grew as fast as the first. Then, I tried to examine the
score of the music.

Keywords: plant stress, Mozart, broccoli sprouts
































53

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Control of Light Quality to Increase Protein Contained in Euglena


Yuma Bono , Yuta Morikawa , Sawaka Tsusima
1
1
1
Advisor: Fumio Nakaya
2

Tennoji Senior High School
1
Osaka Kyoiku University
2



Abstract
In the near future, protein supply is expected to be deficient due to the rapid population growth and
change in global eating habits by spread of Western diets and increase in meatatarian. As a solution to
this problem, we think euglena can be used as a new protein source. From previous research, euglena
has more than 59 kinds of nutrients and is easy for human beings to digest. Therefore, we focused on
proteins contained in euglena. We hypothesized the amount of those proteins would depend on the
amount of photons or the colors of light that euglena gets. To test this hypothesis, after culturing
euglena, its protein quantification was performed using a spectrophotometer. As a result, we found that
photons and light colors affect the amount of protein contained in euglena.


Keywords: Euglena, Contained Protein, Protein Quantification, Light Quality, Photosynthesis






























54

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Degree of Igusa's Allelopathic Effects on Lettuce
(Make Natural Pesticide from plants)


Funatsu Kanon , Nakata Shion , Mitsuuchi Kou
1
1
1

Tennoji Senior High School
1



Abstract
These days, more and more farmers are using herbicides to protect crops from weeds, which deprive
nutrition that crops need. Using more herbicides, however, might have a negative impact on aquatic,
soil biota and human health in the future. As a strategy for weed management, Allelopathy can be used.
This could eliminate the use of synthetic chemicals for this purpose. Allelopathy is the effect caused by
release of toxic chemical substances when a plant dies and is dried. We regarded Igusa as a material of
a natural herbicide. That does not contain any chemical toxics. That is why Igusa can be used as an
alternative to existing herbicides. This research discusses the power of Igusa's allelopathy on a lettuce.
We hypothesized is that Igusa's allelochemical would be strong enough to scramble over lettuce's
growth. We made floral water from dried Igusa to find to what extent Igusa affects lettuce's species.
Through these experiments, we found that Igusa significantly reduces the growth of lettuce.


Keywords: allelopathy, natural herbicie, Igusa, allelochemical



























55

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Making Antibacterial Sheets by Using Psoralen


Sayui Nakajima , Yu Nakaura
1
1
Advisor: Nanako Takeda
1

Ritsumeikan High School
1




Abstract
In this study, we focused on using the properties of Psoralen to create antibacterial sheets. Most
antibacterial sheets contain alcohol. Also, alcohol can cause inflammation to the skin. Making
antibacterial sheets which are not harmful to the human body help alleviate these problems.
Antibacterial effect was the most found under the condition when concentration of Psoralen was 1/100
and irradiation time was 10 minutes. Also, Psoralen absorbed UV light and combined with DNA extract.
It was found that antibacterial sheets can be made by using Psoralen.

Keywords: Psoralen, antibacterial sheet, UV light, DNA extract
































56

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Why do American Crayfish Prefer Herbal Feed to Animal Feed?
- by analyzing the body changes caused by different feed -


Takafumi Komatsu
1

Tokai University Takanawadai Senior High School
1




Abstract
We started our study with this question: Why do the majority of crayfish which live in a natural habitat
have a red body color? They are supposed to be omnivorous, and the body color of the crayfish is
determined by carotene contained by the food they eat. The purpose of the study is to find any evidence
that the omnivorous crayfish spontaneously eat a lot of carotene-containing food and that it is good for
the crayfish bodies. We divided the crayfish into two groups, one group of crayfish were fed with
anacaris as plant food and the other group was fed with kibinago as animal food. We kept doing this
experiment for a month. As a result, we found the feces of the crayfish which were fed with plant food,
contained calcium carbonate in their feces.

Keywords: crayfish, body color, carotene, food, plant food, animal food, feces






























57

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Stable Method for the Recovery of γ-Poly glutamic Acid in Natto




1
1
Ayana Oshiro , Rin Hagihara
Advisor: Shunji Matsubara
1


1 Nara Women’s University Secondary School






Abstract
γ-Poly glutamic acid (PGA) is a viscose substance contained in natto, a traditional fermented food in
Japan. It exhibits several chemical properties including water-solubility, cohesiveness, and
biodegradability, rendering it useful for applications requiring these properties. However, extracting
PGA is problematic. The amount of PGA that can be extracted is low, and it is unstable. The purpose
of this study was to identify a stable and highly effective method for extracting a large amount of
PGA. We compared the respective PGA yields that were obtained through changing the number of
times the natto was mixed as well as through changing the amount of ethanol that was added. The
results clearly show that when the natto was mixed 300 times and ethanol was added to the PGA
solution at a ratio of 4/5, favorable PGA yields were obtained.



Keywords: natto, γ-Poly glutamic acid (PGA), ethanol, biodegradability






















58

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Finding What Activates White Blood Cells




Ayane Matsutake , Makoto Sako 1
1
Advisor: Kiyoshi Kikezaki
1


1 Nagasaki Minami Senior High School






Abstract
The purpose of our study is to find conditions which activate white blood cells. We did experiments
on the saliva of one person with 0.9% saltwater, Ringer’s solution and temperature. We found that
0.9% saltwater activates white blood cells the best.


Keywords: white blood cell, blood






























59

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Fish Meat Cell Culture : Saving the World with Eggs




Shuta Nishikawa , Moe Shiramizu
1
1
1
Advisor: Akiyoshi Kikezaki


1 Nagasaki Minami Senior High School






Abstract
Our purpose is finding a way to solve the problem of food shortage by cloning fish meat using a cell
culture technique we developed in our school. As a result, we succeeded in growing cells of smallscale
blackfish. We plan to grow more cells in a roller bottle with our medium in the future.



Keywords: cell culture, fish meat, food shortage





























60

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Everybody who wants to study protein come here




Shu Shiramizu , Ryuei Kitagawa 1
1
1
Advisor: Atsutoshi Yokoyama

1 National Institute of Technology, Sasebo College





Abstract

One huge barrier for studying proteins in the biology textbook is a difficulty of
understanding structural constructions. You never get any real images or figures about active site of
enzymes from high school biology textbook. One methodology is proposed in this paper about the
way you study protein structures and those active sites. You can download any protein text data from
Protein Data Bank (PDB) homepage free of charge. The PDB files include the structural coordinate
data of proteins and the data are visualized by QtMG software on your PC. The QtMG is also a free
and safe software as well. You can see and enjoy three-dimensional (3D) structures of your favorite
proteins by rotating, rescaling, coloring and printing. You also can focus on the active site of the
enzymes to get further understanding of enzymatic chemical reactions. You will soon realize the
beauty of any constructions composed of protein framework. You may be able to use your own
pictures of proteins on your academic presentation without any copyright concerns.

Keywords: protein, protein data bank, QtMG
























61

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Oral and Poster Presentation

Section C : Chemistry


Development and investigation on the properties of capsule shell from
tomato cutin and chitosan



Yanit Lunprom , Napussorn Tongpeanpong
1
1
Advisors: Siriporn Nanchai , Thanida Trakulsujaritchok
1
2

Princess Chulabhorn Science High School Chonburi
1
Burapha University
2



Abstract

The objectives of this science project were to develop the biopolymeric capsule shells from tomato cutin
and chitosan and investigate on the properties of capsule shells prepared from different composition
ratios between cutin and chitosan. The capsule shells would be suitable for patients having difficulty in
drinking water after taking the capsule. The development of these capsules from tomato cutin and
chitosan derived from shrimp shell which are the agricultural wastes from food production also provides
the benefit of optimum use of the natural resource. The water resistance and dissolution studies revealed
that the film of cutin and chitosan at the ratio of 1 : 2 exhibited the advantages for capsule shell
application, therefore, this composition ratio was used for capsule shell fabrication. The obtained
capsule shell were compared with the capsule shell from cutin, chitosan and gelatin at the ratio of 1 : 1
: 1 and the commercial gelation product in terms of swelling and dissolution behavior, moisture content,
physical properties and processability. It was found that the biopolymeric blend obtained from tomato
cutin, chitosan and gelatin have strong potential for the application of capsule shell product.


Keywords: Capsule shell, Cutin, Chitosan, Gelatin

















62

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The efficiency of inhibition the growth of weeds by allelopathy extract from
Little ironweed in different solvents


Kasidit Luecha , Manatsanan Kaewmala , Wannida Chueasuriya
1
1
1
Advisor: Suwanna Thima
1

Princess Chulabhorn Science High School Chiang Rai
1



Abstract
The objectives of this project was to compare the efficiency of weed eliminating allelopathy extracts
from Little Ironweed (Vermonia cinerea (L.) Less.) in different solvents. This project was conducted in
2 steps. Step 1, the extraction of allelopathy from Little Ironweed (Vermonia cinerea (L.) Less.) with
solvents which are distilled in water, Hexane and Ethyl Acetate in the concentration of 1:10 ml, 1:20
ml, and 1:30 ml (extract: solvent). The result showed that allelopathy in Little Ironweed was the greatest
extract by distilled water, followed by Hexane and Ethyl Acetate. Step 2, tested the extraction of
allelopathy from Little Ironweed (Vermonia cinerea (L.) Less.) by taking the extract to test the inhibition
of seed germination and see the growing progress. There were two kinds of seed in this experiment
which are Gold Beard Grass (Vermonia cinerea (L.) Less.) and Sensitive Plant (Mimosa pudica L.) Two
kinds of seeds were soaked in the extract that had different concentrations for 12 hours. After that,
researchers planted the weed seeds that had been soaked on Petri dishes that had 10 mg of magic crystal
soil and then added 20 weed seeds per dish to find the percentage of inhibiting germination and left the
dish under fluorescent light for 8 hours per day for 14 days. The result showed that the extract from
Little Ironweed (Vermonia cinerea (L.) Less.) with distilled water in the concentration of 1:10 ml can
inhibit the growth of both kinds of weed on the average percentage of 73.38%Secondly, the extract
from Little Ironweed(Vermonia cinerea (L.) Less.) with Hexane in the concentration of 1:10 ml can
inhibit the growth of both kinds of weed on the average percentage of 64.45%.Lastly, the extract from
Little Ironweed(Vermonia cinerea (L.) Less.) with Ethyl Acetate in the concentration of 1:10 ml can
inhibit the growth of both kinds of weed on the average percentage of 54.09%


Keywords: Allelopathy substance, Vermonia cinerea (L.) Less., Mimosa pudica L., Vermonia cinerea
(L.) Less., distilled water, Hexane and Ethyl Acetate

















63

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The effect of cayenne pepper and white holy basil on eliminating ants and
mealybugs


Pusun Rattanakaew , Panupong Praipurksa
1
1
Advisors: Natrapee Chu-on , Somkiat Kerdlong , Nilubol Nuanjunkong
1
1
2

Princess Chulabhorn Science High School Trang
1
Thaksin University
2


Abstract

The ants and mealybugs are interrelated species, the ant protect the mealybugs from their enemies while
the mealybugs feed the ants with the nectar obtained from the plant. There two individuals cause the
nuisance and annoyance to human. Accordingly, the project was aimed to investigate the solvent type,
the optimal extraction time and the ratio of cayenne pepper and white holy basil on eliminating the ants
and the mealybugs. The results showed that 95% ethanol was the most effective solvent. The most
appropriate time for extraction was 72 hours. The pepper and basil at a ratio of 3:2 was the best
extraction for getting rid of the ants and the mealybugs. This insight knowledge could be applied for
developing the insecticide product.


Keywords: The effect of extraction on eliminate, ants, mealybugs
























64

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Dye wastewater treatment
based banana fiber. cellulose aerogel



Kreeta Chanakarn , Natdanai Prachnakorn
1
1
Advisors: Piyada Guedduaythong , Sujittra Poorahong
2
1

Princess Chulabhorn Science High School Nakhon Si Thammarat
1
Walailak University
2



Abstract

Dye wastewater treatment with cellulose aerogel from banana fiber was an innovation created with the
objectives to study the dye adsorption properties of banana fiber and produce a cellulose aerogel from
banana fiber that can remove the dye contaminated in water and also to study the efficiency of banana
fiber and cellulose aerogel in the reducing amount of dye contaminated in water. The optimum
adsorption conditions were studied in term of the adsorbent amount (0.100 to 0.300 g), initial
concentration of methylene blue (5.00-100 mg/L) and adsorption time (5-90 min). The Ultraviolet-
Visible spectrophotometry technique was used for measuring the absorbance of methylene blue
solution. The study found that the best adsorption capacity of methylene blue dye by using banana fibers
was 0.500 mg/g. Furthermore, the complete adsorption occurred within 60 minute when using 0.200 g
of the adsorbent (banana fiber). The isotherm of methylene blue adsorption was consistent with
Freundlich isotherm (R = 0.9845). From the study, the 1/n value was equal to 0.8191 which was
2
between 0-1, this meant that banana fiber was the single or multiple layer adsorbents. When study the
adsorption efficiency to the methylene blue dye between the banana fiber and the cellulose aerogel, it
was found that the cellulose aerogel provided adsorption capacity (5.40 mg/g) but banana fiber provided
adsorption capacity (0.500 mg/g). In which kinetic of Methylene blue adsorption by cellulose aerogel
conforms the pseudo-second order kinetic model.

Keywords: dissolved dye , cellulose aerogel , banana fiber , adsorption kinetic , wastewater treatment
1
2
3
4
5


















65

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The comparison biological performance of Alleopathy extract between
Chromolaena odorata, and Ruellia tuberosa to inhibit germination, and
growth of Echinochloa colona


Saranyaporn sombut , Supitchaya Japa
1
1
Advisors: Watcharaporn Saenna , Kriengkrai Ponhong
2
1

Princess Chulabhorn Science High School Buriram
1
Mahasarakham University
2



Abstract
This work has objective to test biological performance of Alleopathy extract between Chromolaena
odorata and Ruellia tuberosa to inhibit germination, and growth of Echinochloa colona. In parts of
methodology, we have separated into 2 sections which are extraction Alleopathy from Chromolaena
odorata ,and Ruellia tuberosa ,and testing efficiency of inhibition germination and growth of
Echinochloa colona. From the experiment, we have encountered tertiary substance 4 groups which are
Alkaloid, Phenolic, Flavonoid, and Terpenoid of Alleopathy. The Chromolaena odorata extraction has
better efficiency than Ruellia tuberosa extraction. Chromolaena odorata extraction at concentration
2000, 4000, and 6000 ppm are able to inhibit growth of Echinochloa colona for 38.49%, 58.47%, and
70.49% respectively. Moreover, injection of extraction concentration 6000 ppm into the rice farm. We
find both extract do not influence to the germination, and growth of rice when compare with control
condition. Therefore, Chromolaena odorata, and Ruellia tuberosa extract are able to inhibit
Echinochloa colona, and have no side effect to the rice.


Keywords: Alleopathy, Chromolaena odorata, Ruellia tuberosa, and Echinochl





















66

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The Study of Thai Herbs Ability in Growth Inhibition of Cancer Cells



Thanachai Dowsuksri
1
Advisors :Pornpan Chomwong , Boon-ek Yingyongnarongku
1
2
Chopaka Thongbamrer , Attapon Duongsawan
2
2
Onuma Phankhat , Supalak Huayhongthong
3
4

Princess Chulabhorn Science High School Pathumthani
1
Ramkhamheang University
2
The Demonstration School of Ramkhamhaeng University
3
Princess Sirindhorn's College
4

Abstract


The purposes of this research study were to effect of cancer inhibition from thai herbs to develop to be
used as a medicine . In this experiment, dried herbs were soaked in hexane and ethyl acetate
respectively, then filtered and evaporated and using the crude extracts to cancer inhibition test. The
experimental results, it was found that the crude extracts from Artocarpus(mahad), Chromolaena
oderate (euphorbiaceae) and Tectona gradis (teak) in the ethyl acetate layer have the effect of
inhibiting cervical cancer (Hela), prostate cancer (PC3) and lung cancer (H460) by comparison with
the standard cancer drugs. (doxorubicin)
Keywords: herbs, thai herbs, cancer, cancer inhibition test























67

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Comparison of phosphate removal efficiency from wastewater using
modified kaolin prepared from reflux and microwave methods


Jitsupa Intawong , Tudpicha Keawphraserdkul
1
1
Advisors: Piyamas Charoenchai , Wipharat Chuachuad Chaiyasith
1
2

Princess Chulabhorn Science High School Phitsanulok
1
Naresuan University
2



Abstract
The main objective of this research is to study the effectiveness of kaolin which was improved its
surface properties through the reflux and microwave methods using hydrochloric acid at different
concentrations and reaction time in order to use for the development of kaolin as an adsorbent material
for wastewater treatment and to reduce the pollution in the environment. Initially, using the reflux and
3 hours, with the addition of
hydrochloric acid at concentrations of 1, 1.5 and 2 M. To thoroughly determine the effectiveness of the
concentrations, different heating times were examined, and the time at 45, 60, 90 minutes were studied.
In order to determine the adsorption capacity in synthetic wastewater, the ascorbic acid was applied.
The results showed that hydrochloric acid concentrations and the change in the incubation time
increased the efficiency of kaolin and its ability to adsorb phosphate ion. By applying the microwave
method at concentration 2 M of HCl, it was observed that the incubation time of 90 minutes has the
highest adsorption capacity of phosphate (89.37%) and it is higher than the modified kaolin by reflux
method. From the physical characteristics of kaolin, it was observed that kaolin was brittle, contained
small pores and had a rough texture. In addition, by using the reflux and microwave methods on kaolin,
it also improved its efficiency to adsorb phosphate into 6 hours which the microwave method can adsorb
up to 99.41% of phosphate. In addition, kaolin was also applied to the general wastewater treatment. It
was found that the modified kaolin by microwave method can adsorb up to 66.86% with a good result
for using as an adsorbent material.


Keywords : Kaolin, Efficiency, Phosphate, Wastewater, Environment

















68

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Food Additive from Freeze-Dried Nam Dok Mai Golden Mango



1
1
Yanisa Darunsri , Pichaya Srihera , Pongthorn Khaobang
1
Advisor: Nattapon Klinput 1


1 Princess Chulabhorn Science High School Phetchaburi



Abstract

Nam Dok Mai Golden Mango (Barracuda) is an agricultural product of Prachuap Khiri Khan. It is a
golden yellow color, sweet and fragrant which is exported to Japan, China and Malaysia. The shipping
time to each country is about 14 days or longer so it's overripe and has to be discarded. This experiment
chose the leftover mangoes to add value, extend the storage time and process Nam Dok Mai Golden
Mango to be the food additive by freeze-drying. Firstly, Nam Dok Mai golden mangoes were blended
and extracted to be juice. Next, added maltodextrin at 3%, 5%, 7% into mango juice and another one
without maltodextrin follow by freeze-dried for 72 hours. Then tested the mango powder properties and
compared with mango juice afterwards. The results of the study showed that mango powder which
added maltodextrin at 0% 3% 5% and 7% had a percentage of quality at 10.94%, 12.10%, 14.98%, and
12.02%, respectively. After that, the properties of mango powder with 5% maltodextrin was clearly
investigated. Further investigation showed that the sweetness of mango powder was 14.4 brix and the
water activity was 0.25 which indicated that microbes couldn't grow in this mango powder. Moreover,
the total color difference between ripe mango juice and mango powder was 67.71, the solubility of
mango powder was 3.33 minutes and the amount of beta carotene in mango powder was 20.48 mg/g.
After testing an affective test of mango powder and the other, the satisfaction result of mango powder
was 16.60 while the result of additives was 16.36. Mango powder has more satisfaction than products
in the market (95% of confidence level). It is concluded that mango powder added with maltodextrin
5% has the most similar properties to ripe mangoes and the freeze-dried method can preserve and extend
the shelf life of Nam Dok Mai Golden Mangoes.

Keywords: Nam Dok Mai Golden Mango, Freeze-dried, Mango Powder


















69

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The composite rubber flooring tile reinforced with natural fibers and
polyethylene


Krittaya Sukhampa , Suphatsron Churat
1
1
Advisors: Patcharaporn Wichasan , Ukkadate Moonart
1
1

Princess Chulaborn Science High school Mukdahan
1



Abstract

The composite rubber tiles using natural cellulose fibers mixed with rubber and polyethylene. The
objective is to develop an appropriate ratio of rubber and cellulose to make tiles and compare the
mechanical efficiency of composite rubber tiles reinforced with natural cellulose fibers mixed with
natural rubber and polyethylene waste. By mixing rubber using RSS3 sheet and polyethylene at a ratio
of 80: 20 at a temperature of 170 degrees Celsius By using INTERAL MIXED: MX75 for 10 minutes
and mixed with 1.25, 2.5 and 5 gram of cellulose for 5 minutes, then mixed with a two-roller grinder
with stearic acid: ZnO: Mercaptobenzothiazole: Diphenylguanidine: Sulfur At a fixed ratio of 2: 3: 0.5:
0.2: 3 and then pressed by hot pressing at 150 degrees celsius for 3 minutes and 30 seconds. The rubber
sheet is 8 x 8 x 5.5 cm. Composite rubber tiles, RSS3 rubber and polyethylene which is reinforced with
natural cellulose fibers at a ratio of 5 is suitable for flooring due to the highest strength when tested in
accordance with ASTM D 2240ISO7619-1 when testing water absorption, it is found that the rubber
tile does not absorb water and when testing compressive strength according to ASTM D 395 Method
BIO815-1, it is found that the compressive strength can be supported the most. In the acid-base testing,
it is found that the weights did not change but the size of all rubber tiles have changed a little bit.
Therefore the fourth tile is suitable for making floor tiles.

Keywords: natural cellulose fibers, rubber, and polyethylene





















70

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Effect of eggshell and marl addition on the properties of cruse pot for
hot saltpot application for postpartum women


Kwanchanok Jongpattana , Saowalak Sangkhao
1
1
Advisors: Wipawee Srihanon , Thanakorn Wasanapiarnpong
1
2

Princess Chulabhorn Science High School Lopburi
1
Chulalongkorn University
2



Abstract
The project on Alternative materials to make a convenient pot for postpartum women is intended to
compare the ratio between clay to renewable material and compare the properties of the pot-based
material over salt, since the chicken egg shell is a residual material whose main composition is calcium
carbonate to decompose into some carbon dioxide. This allows for porosity in the terracotta body. The
marl is a white clay with naturally occurring calcium carbonate elements, which also helps to create a
perforation in the terracotta. That's why experiments are mixed in the clay to improve the porosity
properties. The experiment replaced Ratchaburi clay used for pottery with finely ground chicken
eggshells or inflatable pencils in 0 25 and 50 by mass to compare the ratio between Ratchaburi clay and
substitute. The clay is mixed with water in a ratio of approximately 18-22 percent by the mass, which
makes the clay texture to be sticky for molding, then molded in a metal mold to achieve a square shape
of 50x50 mm, approximately 10 mm thick, dried in the air for 1 day, and then burnt at different
temperatures which were divided into 800, 900, 1000°C. Take the samples of the burned specimens to
test the properties, comparable to the ratchaburi clay that does not fill the eggshell or marl used to mold
the pot over salt. 1. Measure color measurements with Konica Minolta Chroma Chroma Meter CR-400
to determine the color found in the material sample. 2. Density Measurement The replacement material
is weighed based on the principle of archimedes. 3. Measure thermal conductivity with transient hot
bridge - Thermal Conductivity Meter to know the timing of the most efficient thermal conduction of
renewable materials. 4. Strength test with 3-point bending strength test to study the strength of the salt
pot substitute. This will be the right ratio and properties to make the substitute for the salt pot.

Keywords: cruse pot, hot saltpot, chicken eggshell, marl
















71

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The wastewater treatment machine nano zinc oxide



Jirart Nganrungreung , Siriyakorn Theprat , Nattiwut Wisedsri
1
1
1
Advisors: Namthip Srikeaw , Surasuk Boontima
1
1

1 Princess Chulabhorn Science High School Loei




Abstract

This project aims to invent the wastewater treatment machine and to study the performance of coliform
bacteria disinfection by mixing clear coating with Nano ZnO coated on a propeller and inside the bucket.
We use ultraviolet light to catalysis Nano ZnO and disinfects coliform bacteria. The activated charcoal
box was used to absorb the waste gas from hydrogen sulfide (H2S) by sucking the air to pass through
the tube then the activated carbon that contains inside the tube will absorb the waste gas. From the
measurement of water quality by measuring pH, DO, hydrogen sulfide concentration and total coliform
bacteria every 30 minutes for 6 times, the results showed that pH increase to 6.02 from 4.22, DO
increase to 4.02 mg /l from 2.31 mg /l, the total of coliform bacteria decreased and the concentration of
hydrogen sulfide decrease after absorption. The information represents that the wastewater treatment
machine can treatment wastewater contamination coliform bacteria and absorb the hydrogen sulfide
effectively.

Keyword: Nano ZnO, Quality of water, The wastewater treatment machine
























72

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Production of Roofing Tiles from Rubber Mixed with SiC from Rice Husk
Ash and Titanium Dioxide-coated


Kaesinee Leesen , Beebeefatimee Muna
1
1
Advisors: Sathit Buadam , Ekwipoo Kalkornsurapranee
1
2

Princess Chulabhorn Science High School Satun
1
Prince of Songkla University
2



Abstract

This project aims to study (1) whether rice husk ash can help increase hardness and thermal insulation
for rubber tiles and (2) whether a coating of titanium dioxide can help reduce the temperature in the
house. From the experiment, the amount of rice husk ash used is 0, 20, 40, 60 and 80 phr. The types of
mechanical property test studied are tensile strength, 100% modulus, hardness and elongation at break.
The results show that roof sheets with 60 phr rice husk ash have the best mechanical properties.
Consistent with the insulation test results. After that, rubber sheets containing 60 phr rice husk ash was
sprayed with 15% of TiO2, were the most effective used for insulation by more than 21% and caused
the air temperature under the tiles to decrease 2.97% compared to the 60 phr roofing sheet.

Keywords: rubber tiles 1, thermal insulation 2, mechanical property 3, temperature decrease 4
























73

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Phosphate absorbing material in washing water


Wittawin Punpeng ,Natthakit Thongchana
1
1
Advisor: Montien Songserm
1

Chonrasadornumrung
1


Abstract
The purpose of this project is to study the suitable materials for phosphate adsorption in washing water
and find a suitable binder to mold the material and absorb the phosphate in waste water with high
phosphate content which causes the plants to grow too much. We studied the materials for using to
absorb phosphate substances by using many materials Group 1 is egg shell, mussel shell and fly ash.
Group 2 is Chamchuri tree bark and Mangosteen shell. Group 3 chicken bones and pork bones. Finding
suitable materials for phosphate absorption is studied by Dissolve the detergent in water 10 grams of
each material were absorbed for 30 minutes. Tested by standard phosphate test kit, found that egg shells,
mussel shells and fly ash can reduce the amount of phosphate in the water. Then took the eggshell
Mussel shells and fly ash to burn by kiln with the temperature of 800 C for 3 hours and tested it again
๐
by dissolving the detergent in water until having a phosphate value of 5 mg / l when using egg shells
to burn. Burned mussel shells and 10 grams of fly ash absorbed for 30 minutes. It was found that the
detergent solution when adding the burned egg shells. Flaming mussel shells and fly ash had the
phosphate values reduced to 0.25, 0.5, 1.0 mg / l respectively. The coagulation of all 3 absorbent
materials with quicklime, plaster and tapioca flour, it was found that mussel shells and fly ash use
tapioca flour to combine well with for the eggshell, the plaster is needed to combine well. The use of
absorbent materials was tested by preparing 6 liters of washing water to measure 2.5 mg / l of phosphate.
When 3 types of absorbent were added in 30 minutes, we found that phosphate in washing water was
reduced to about 0.5 mg/l

Keywords: phosphate adsorption, egg shells, mussel shells, fly ash




















74

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Degradable time reduction of impact resistant material from recycled
polystyrene foam


Phanthira Phumpour , Waritsara Srinuaneiad
1
1
Advisor: Panita Sumanatrakul
2

Paphayomphittayakom School
1
Thaksin University
2




Abstract

The aims of this research were to study the effect and mechanical properties of mixing recycled
polystyrene foam with latex compound to form an impact resistant application and reduce the
degradation time of the material. The characterizations for the mechanical properties of the impact
resistant material were tensile strength, elongation at break, modulus, specific gravity, hardness,
weathering resistance and thermal degradation. The morphologies and functional groups of the mixture
were investigated and also compared to the original polystyrene foam. The results found that the
appearance of the mixture was semi-hard and its specific gravity was less than the original latex
compound and original polystyrene foam. Hence, its weight was very light. The tensile strength and
elongation at break slightly reduced when the amount of recycled polystyrene foam was increased.
When recycled polystyrene foam was added to the mix, 300% modulus and the hardness of the impact
resistant application increased. Herein, the mixture obtained a lower thermal degradation temperature
than the original polystyrene foam. In addition, morphologies before and after a weathering test found
that increasing the amount of recycled polystyrene foam affected the increasing porosity and fracture
at the surface of the specimen, respectively. Furthermore, the weathering of recycled polystyrene foam
resulted in the formation of carbonyl groups in the regions of 1500–1800 cm and hydroxyl at 3000–
-1
3800 cm in the FTIR spectra, as well as high carbonyl index (CI) and hydroxyl index (HI). Therefore,
-1
there was a greater degree of degradation. It can be concluded that the mixture of recycled polystyrene
foam and latex compound resulted in optimum mechanical properties to form an impact resistant
material. Moreover, adding recycled polystyrene foam with latex compound can also decrease the
degradation time of the material.

Keywords : degrade, impact resistance material, latex compound, polystyrene foam, recycle
















75

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The study of the amount of chemical contaminated vegetables in group of
Pyrethroids, Organophosphates and Carbamates from Ban Nong O
community, Mae Chan district, Chiang Rai province.


Pakprim Suthep , Palita Kuenphet
1
1
Siriphon Apipatthanachai
1
Advisors: Boonnada Yodkeaw , Kanda chuangchai
1
1

Maechanwittayakhom School
1



Abstract
Ban Nong O Community, Mae Chan District, Chiang Rai Province is an organic community
that has an area of farming. It is next to the one using chemical pesticides group of Pyrethroids
Organophosphates and Carbamates, which may cause contamination of vegetables. The
purpose of this research is to study organic pesticides, Carbamate sites and Pyrethroids
contaminated with indigenous vegetables in the Nong Oran community from the area where
the above chemicals are used. The area using chemical pesticides group of Pyrethroids
Organophosphates and Carbamates, it is center to sample the surrounding area to check. The
sample spacing from the area using chemical pesticides 100 meters , 500 meters , 800 meters,
by checking through inspections GT Kit to detect chemical pesticides group of
Organophosphates , Carbamates, and check PY test kit detect chemical pesticides group of
Pyrethroids , tested with Lettuce , Tomato , Eggplant , Local eatable plant(Pak Plung) , Sacred
basil , Chinese cabbage and Cantonese lettuce. The results showed that the test kit PY test kit
to check for chemical pesticides group of Pyrethroids in vegetable samples and area in all. The
test tubes were darker than the controlled ones. Tested kit GT Kit and checked for chemical
pesticides group of Organophosphates, Carbamates in vegetable samples and area in all. The
test tubes were lighter in color than the controlled ones. The local vegetables grown around the
area using chemicals. Undetected chemical pesticides group of Pyrethroids Organophosphates
and Carbamates.

Keywords: Pyrethroids, Organophosphates, Carbamates, GT Kit, PY test kit, Organic















76

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Paraffin vapor flames combust independently at a constant distance caused
by the speed of falling vapor and the local oxygen environment


Kouyou Fu
1
Advisor: Nobuzumi Iwakawa
1

Bunkyo Gakuin University Girls’ High School
1




Abstract

Paraffin is a complex hydrocarbon that is commonly used in candles. Paraffin vapor is known to
combust spontaneously, and I found that it burns twice during the phenomenon, I wanted to find the
reason of the twice combustion. The objective of our research is to better understand the mechanism of
this spontaneous combustion. As a result, we found that the vapor combustion occurs in two stages, and
two flames are produced in quick succession. The distance between the maximum height of the first
flame and the starting point of the second flame was fairly constant. Then we repeated the trial while
suppressing each of the two flames in turn to determine if there was a dependent relationship between
them. We can conclude that the probable reason for the “constant distance between the two flames” is
that the speed of the falling paraffin vapor is constant so that each trial produced similar results. And
we found that the two flame is independence so I consider that the two combustion ignites with different
oxygen and the reason of the two combustion is that the paraffin vapor separate during the dropping,
part of them burn at first, then the other burns the second time.


Keywords: paraffin, vapor, spontaneous, combustion, independence, ignite, separate























77

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



A Study on the Luminous Ability of Vargula Hilgendorfii Luciferin

Kanon Kitazono , Chika Goto , Ito Murakami , Isoda Yuki and Ezumi Yuto
1
1
1
1
1
Advisor: Yuki Kutsunugi
1

Hiroshima University High School
1

Abstract
We have been studying Vargula Hilgendorfii, or the sea firefly, which is a tiny sea creature that emits
beautiful blue light. Our purpose is to find an effective method to maintain their luminescence for an
extended period of time without losing the luminescence intensity. To achieve this goal, we have set
two sub-goals. The first is to identify the optimum conditions for Vargula Hilgendorfii to emit
light. They emit light when their Vargula Hilgendorfii Luciferin (substrate) and Vargula Hilgendorfii
Luciferase (enzyme) are released in the sea and the substrate is oxidized by the oxygen in the sea water,
catalyzed by luciferase. Previous studies have found that the optimum water temperature for the
reaction is 4℃. It is also known that they emit stronger light in a water with the pH of 7.8 than in a
water with the pH of 6.0. In our study, we attempt to find the optimum combination of the temperature
and the pH of the water. The second sub-goal is to quantify the luminescence intensity more
easily. Previous studies used the luminometer or photometer to quantify the intensity, but they are
available to us because of their expensiveness. Instead, we have been trying to establish another method
that uses an ordinary digital camera and a computer software called Makali'i, which is widely used in
the field of astronomy. So far, we have succeeded in the quantification. We are now trying to clarify
the effects of water temperature on the luminescence time and intensity. We also plan to study the
effects of the pH of water on the luminescence time and intensity. In the end, we will compare the
results we have gained with our new quantification method with those gained by the luminometer and
verify the reliability of our method.


Keywords: Vargula Hilgendorfii, luminescence, optimum condition, quantification,




















78

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The time dependent change of redox reaction under various conditions



Kotaro Sato
1
Advisor: Nanako Takeda
1

Ritsumeikan High School
1




Abstract
This research examines how changes in the amount of NaOH or stirring conditions affects the speed
and time of redox reaction using the chemical traffic light experiment. How to mix and change the
substances types and amounts were mainly discussed in this research. In most of the experiments, the
time and speed changed. The mixing method was found to greatly affect the redox reaction of solutions.
These experiments are redox reaction, so having air in the bottle is very important for these experiments.
The method by which to mix the solutions so that they would come in contact with air was determined.
The substances were affected by the reducibility in these experiments. For example, there are many
types of sugar and not all sugars possess reducibility. Furthermore, the amount of substances was very
important, because concentration was affected by it. These factors affected both the speed and time
redux reactions conducted in this research.


Keyword: redox reaction high


























79

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The pigment with the Highest Electricity-Generating potential



Ayu Abe , Ako Sato
1
1
Miori Nambu , Misaki Maita
1
1
Advisors: Michie Nagata , Koichi Mukasa
1
1

Sapporo Kaisei Secondary School
1


Abstract
We are making "Dye Sensitized solar Cell (DSSC) now. DSSC is a kind of solar cell and using TiO2 as
photocatalyst to absorb Ultraviolet Radiation also uses pigment to expand the sensing range and utilize
Visible Radiation.We propose as our hypothesis that chlorophyll is the best pigment. The reason we
focused on chlorophyll was that we were interested in the excitation state or the ground states of the
dye and the wavelength of sunlight. We have an idea that chlorophyll is contained in plants because
chlorophyll can use sunlight most efficiently for exciting electronics.
We found some problems to solve through the process so far. One of those is the shortage of generated
voltage. There are too little to compare to in some kinds of pigment. Therefore, future perspective is
reviewing the experimental method and validity assessment. If the validity of this research is confirmed,
it can boost the spreading of green power. Green power (electricity) is still currently expensive;
however, the DSSC can generate electricity more efficiently at a lower cost.

Keywords:Dye Sensitized Solar Cell, chlorophyll, excitation state, TiO2, wavelength of sunlight























80

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



A New Method of Preventing Mold Using Water Repellent Coating


Rio Kamae
1

Tokai University Takanawadai Senior High School
1




Abstract
Mold can grow easily everywhere. It spoils the look of things and causes various health problems. Mold
is difficult to remove. Typically, we remove mold after finding it. It takes a lot of work. So, it is
important to develop a long-term mold prevention method to reduce cleaning. I hypothesized that we
can do long-term mold prevention by using water repellent. This is because mold needs water to live. I
did two experimental set ups. First, I reproduced conditions for mold in bathrooms. Normally, mold
grows on sealant. I made two samples that were made of sealant. I coated silicone, a kind of water
repellent, on one sample. I wet both with water, sprinkled black mold spores on the sealant and kept the
humidity high. As a result, neither had mold growing on them. Then, I reproduced wear and tear on the
sealant. I painted sealant to the side of the case and scratched the sealant on one half of the box using a
box cutter. I put a petri dish with mold spores and a petri dish of water into the case, to keep the humidity
high. As a result, I found mold in only the scratched sealant. So, I think new sealant is difficult for mold
to grow on, but worn sealant is easy for it to grow in. Next, I tested mold prevention for wood. I
prepared two pieces of Japanese red pinewood. I coated one sample with water repellent. I wet both,
and put both with mold and water into a case. As a result, mold did not grow on the piece of wood
coated with water repellent. So, this shows that mold cannot grow on wood with water repellent.


Keywords: mold, prevention method, water repellent, silicone























81

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The differences in ingredients between Thai and Japanese soy sauce and
fish sauce


1
1
Yumeno Nakajima , Yuri Yoshida
Advisor: Noriyuki Matsuura
1


1 Nara Women’s University Secondary School,



Abstract


This study compared the protein and salt content in two types of soy sauce and two types of fish sauce:
dark soy sauce, light soy sauce, “shottsuru” (Japanese fish soy sauce), and nam pla. Dark and light soy
sauce are typically used in cooking. The amount of protein was determined by measuring Kjeldahl
nitrogen, and salt content in each sauce was determined using the Mohr method. Among the four types,
fish sauce exhibited higher salt content than soy sauce, and the sauces that were higher in protein
contained lower salt content.


Keywords: soy sauce, fish sauce, protein, salt, Kjeldahl nitrogen, Mohr method



























82

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



The wastewater treatment machine

1
Jirart Nganrungreung , Siriyakorn Theprat , Nattiwut Wisedsri
1
1
1
1
Advisors: Namthip Srikeaw , Surasuk Boontima

1 Princess Chulabhorn Science High School Loei


Abstract

This project aims to invent the wastewater treatment machine and to studies, the performance of
coliform bacteria disinfection by mixing clear coating with Nano ZnO coated on a propeller and inside
the bucket. Use ultraviolet light to catalysis Nano ZnO and disinfects coliform bacteria. The activated
charcoal box was used to absorb the waste gas from hydrogen sulfide (H2S) by suck the air pass through
the tube then the activated carbon that contains inside the tube will absorb the waste gas. From the
measurement of water quality by measure pH, DO, hydrogen sulfide concentration and total coliform
bacteria every 30 minutes for 6 times, the results showed that pH increase to 6.02 from 4.22, DO
increase to 4.02 mg /l from 2.31 mg /l, the total of coliform bacteria decreased and the concentration of
hydrogen sulfide decrease after absorption. The information representing that the wastewater treatment
machine can treatment wastewater contamination coliform bacteria and absorb the hydrogen sulfide
effectively. Keyword: Nano ZnO, Quality of water, The wastewater treatment machine





























83

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Concrete Carbonation and Our Lives

1
Hane Moe , Fujii Kaho
1
1
1
1
1
Advisors: Takuya Ohata , Tzuzuki Keita , Eguchi Keiko , Kaneko Michiko

1 National Institute of Technology Toyota College

Abstract

We have been learning construction materials. The reinforced concrete is cheap and of high durability.
It is used in many things such as skyscraper, civil structure, but the concrete causes corrosion problems
in reinforced steel. The concrete is important for our lives so we believe that we must solve the problem.
We studied and experimented concrete carbonation (Ca(OH)2+CO2→CaCO3+H2O). Concrete,
CH3COOH and phenolphthalein solution were prepared.

We created concrete as follows.
1: Set water, cement, fine aggregate and coarse aggregate (Tabel.1)
2: Mixed them and let the air bubbles out. (Use iron mold for compressive strength test, Toro boat)
3: Consolidated concrete.
4: Cut concrete (A) and put in CH3COOH.
3
Tabel.1: Mix proportion of concrete (1m )

W/C s/a Water Cement Fine Coarse AE
aggregate aggregate entraining
agent
3
3
3
3
3
(%) (%) (kg/m ) (kg/m ) (kg/m ) (kg/m ) (g/m )
0.52 45.7 194 373 778 965 0
The concrete was put in CH3COOH for a week. CH3COOH pH is 3, originally concrete pH is 14. The
carbonation depth of concrete was 30mm. The right picture (C) shows normal concrete, but the middle
picture (B) shows carbonated concrete. Concrete carbonation proceed quickly, so it is clear to check
concrete carbonation frequently.



Keywords: Concrete, Concrete Carbonation, Corrosion in Reinforced Steel












84

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Identification of volatile compounds improving the hydroponic technique
for strawberry

1
1
Kenta Shimada , Riku Kitajima
Advisor: Izumi Sasanuma
1

1 National Institute of Technology, Oyama College


Abstract

Hydroponic systems for vegetable production are nowadays essential to maximize productions and
increase yields. However, the technical issues concerning control microbial diseases and pests, and the
low level of flavor have not yet been improved. Here we report volatile organic compounds (VOCs)
released from companion plants can be used as chemical signals providing better quality for
strawberries during the hydroponic culture. First, we identified the chemical stimuli emanating from
companion plants with strawberries via spectrometry analyses and found volatile organic compounds
(VOCs) being responsible for the strawberry growth. The strawberries grown with tomato and lavender
as companion plants were significantly higher in growth than non-companion strawberry. Especially,
tomato VOCs stimulated strawberry growth. Therefore, the VOC was identified the chemical structure
by using FT-IR, and NMR spectroscopy analysis. Keywords: volatile organic compounds, companion
planting, strawberry





























85

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Let’s Look at Chemistry Using Color!

1
Nodoka Nakagawa , Mahiru Matsunaga
1
1
Advisor: Atsutoshi Yokoyama

1 National Institute of Technology, Sasebo College


Abstract

When you imagine the “clock reaction”, that must be the “iodine (I2)” clock reaction. Iodine clock
reaction is very well-known and easily prepared to enjoy people by changing solution color. Some high
school uses this reaction in chemistry class to study redox reactions. However, the simple dark iodine
solution color never satisfied us because we wanted to see other “colors” in the clock reaction. Thus,
we found a “formaldehyde” clock reaction and modified the reaction conditions to prepare colors. We
were successfully able to demonstrate the colored clock reaction, and prepared six-colored reactions
(red, orange, yellow, green, blue and purple) to remind people of rainbow. In addition, we joined a
“delivery experiment” as a presenter/organizer and show the rainbow clock reaction to elementary
school kids. Keywords: clock reaction, rainbow color, redox, science show

































86

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”




Oral and Poster Presentation

Section D : Physics




Shape memory composite bag from rubber reinforced with cellulose from
grapefruit peel.

Kanokkorn Permpiumsup , Thipparada Berkban
1
1
Advisors: Kanokrat Singnui , Thorsak Kitttikorn
1
2

Princess Chulabhorn Science High School Nakhon Si Thammarat
1
Prince of Songkla University
2
Abstract

The directors has made of nano-composite bags from rubber, reinforced with cellulose from grapefruit
peel, with cellulose extracted with NaOH, HCl, Acetic acid to obtain cellulose, and then cellulose
extraction by acid method. hydrolysis with Sulfuric acid and ethyl alcohol. Then the fibers were
examined using, and then mixed with rubber using crosslink agent that consist of Potassium hydroxide
solution 0.35% , Sulphur dispersion 1.5% , ZDC dispersion 0.75% , ZMBT dispersion 0.6% , Zinc
oxide dispersion 0.3% at the amount of cellulose are 0%, 1%, 3%, 5%, 10%, 15%, 20% after that test
by using the universal testing machine and test the memory performance at various temperatures with
Dynamic Mechanical Analyzer, then form the bag with Blown Film Extrusion technique. From rubber
and grapefruit peel 5%, the maximum tensile strength was 489 N/m , followed by 10%, 15%, 20%, 0%,
2
respectively, with the statistical significance of ANOVA 0.05 and the results of the performance tests
found. That the composite bag 5% of rubber and grapefruit peel had the highest tensile strength at
various temperatures at 100% stress, 150% stress and 200% stress, which were 98.6%, 98.1%, and 97%
respectively at ANOVA 0.05 statistics, followed by 10%, 15%, 20%, 0%, respectively. From the results,
it can be concluded that the most suitable ratio is 1% cellulose.

Keywords: natural rubber , cellulose , shape memory
2
3
1















87

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”



Designing a Blood Alcohol Analyzer Device by Using an Infrared Sensor

Chinnachot Wettayanont , Chaipat Santivimol
1
1
Advisors: Piyanuch Keawaram , Manas Sittishoktram
1
1

Princess Chulabhorn Science High School Phetchaburi
1



Abstract

According to the World Health Organization, alcohol causes about 1 in 20 deaths worldwide every year,
including the problem of drunk driving, violence and disorders of the body that are affected by alcohol.
The government has controlled by establishing alcohol content checkpoints to solve these problems and
using various methods such as an alcohol breath tester and a direct blood test. Also, there is research

indicated that infrared light could be measure alcohol content. So, this project aimed to study and
determine the alcohol content in both blood and water by using the principle of infrared light to operate.
Two types of infrared sensors, CNY70 (950 nm) and IR LED (940 nm) were used to determine the
amount of alcohol in water and blood at various alcohol concentration from 0% - 100% with 10% increment
in order to create the standard graph. The results from measurement showed that the signal from CNY70
sensor closely related to the amount of alcohol in water with 0.995 coefficient of determination (R²).
Therefore, it is a sensor that can measure the differences exquisitely due to the alcohol in the water is
less disturbed than the alcohol in the blood. From this result, this device can be used to measure the
amount of alcohol in water and beverages accurately. However, it should be continued development.

Keywords: Blood alcohol content, Infrared Sensor, Measuring alcohol content






















88