10 Years of Cooperation between Japan and Princess Chulabhorn Science High Schools

Appendix II Academic Cooperation with KOSEN Institutes
1. Toyota KOSEN
2. Nagano KOSEN
3. Sendai KOSEN
4. Kumamoto KOSEN
5. Tsuyama KOSEN
6. Akashi KOSEN
7. Hachinohe KOSEN
8. Ibaraki KOSEN
9. Nagaoka KOSEN
10. Sendai KOSEN

49

Academic Cooperation between Toyota KOSEN
and Princess Chulabhorn Science High School Chiang Rai

Summary the Academic Cooperation
In 2020, 13 representative students in grade 9 from PCSHSCR joined a project
of a video contest on the topic of ‘What can we do for a sustainable society?’ with
students from Toyota KOSEN. Students had the opportunity to use scientific
knowledge to promote how to create a sustainable society and they could improve
their English language in conveying the messages as a means of communication in
making the video together.
School’s Information
PCSHSCR Address Princess Chulabhorn Science High School Chiang Rai
345/ 2 Robwieng Sub-District, Muang Chiang Rai District, Chiang Rai 57000
Website : https://www.pcccr.ac.th/
KOSEN Address 471-8525 National Institute of Technology, Toyota KOSEN
2-1 Eiseicho, Toyota, Aichi, Japan, 471-8525
Website : https://www.toyota-ct.ac.jp

50

Academic Cooperation between Nagano KOSEN
and Princess Chulabhorn Science High School Phitsanulok

For the academic cooperation between PCSHS Phitsanulok and Nagano KOSEN,
the first visit at PCSHS Phitsanulok was started in August 2019 by the visit of Dr.
Nobukazu Doi, the president and Mr. Frank Kabel, the international coordinator of
Nagano KOSEN to observe the school, and to discuss about an MOU. On 1 October
2019, the MOU signing was held in Nagano KOSEN in Japan. Then, the academic
cooperation started by invitation of Nagano KOSEN one student and one teacher of
PCSHS Phitsanulok to participate in KOSEN Robot Contest in Tokyo (ROBOCON).
They had a good experience in attending the classes at Nagano KOSEN, as well as,
in visiting the learning resources of Nagano City. Later on, students and teachers
of Nagano KOSEN came to participate in the academic activities in Thailand-Japan
Student ICT Fair 2019 in Mukdahan. Academic cooperation activities between
PCSHS Phitsanulok and Nagano KOSEN has good cooperation.

The COVID-19 pandemic has impact on student exchange. However, the academic
cooperation activities were carried out online.

School’s Information
Princess Chulabhorn Science High School Phitsanulok
86 Moo 4, Makhamsung, Mueang, Phitsanulok 65000 Thailand
Website : http://www.pccpl.ac.th/
Tel: 055-245115

Nagano KOSEN
716 Tokuma, Nagano City, 381-8550 Japan
Website : https://www.nagano-nct.ac.jp/english/
Tel: +81-26-295-7003

51

Academic Cooperation between Sendai KOSEN
and Princess Chulabhorn Science High School Lopburi

The academic cooperation between PCSHS Lopburi and Sendai KOSEN started
with the first visit of contact person and representatives of Sendai KOSEN to PCSHS
Lopburi in November 2019. They had observation of the school and we had discussion
about the MOU. Due to the COVID-19 pandemic, the MOU signing ceremony
between Sendai KOSEN and PCSHS Lopburi was held online on 4 June 2021. It is
hoped that the academic cooperation activities will be carried out when the situation
of the COVID-19 pandemic becomes better.
School’s Information
Princess Chulabhorn Science High School Lopburi
216 Village No.1 Huai Pong Sub-District, Khok Samrong District Lopburi 15000
Website : https://pccl.ac.th/pccl/
Sendai KOSEN, Hirose Campus
4-16-1 Ayashi-Chuo, Aoba-ku, Sendai-shi, Miyagi 989-3128, Japan
Website : https://www.sendai-nct.ac.jp/english/
Sendai KOSEN, Natori Campus
48 Nodayama, Medeshima-Shiote, Natori-shi, Miyagi 981-1239, Japan
Website : https://www.sendai-nct.ac.jp/english/

52

Academic Collaboration between Sendai KOSEN
and Princess Chulabhorn Science High School Satun

The MOU of academic cooperation between PCSHS Satun and Sendai
KOSEN was signed by online on 4 June 2021. The cooperation agreement is covered:
exchange of students, lecturers, and faculty members; co-operative research activities,
exchange of information and materials in those fields which are of interest to both
institutions; or other activities agreed upon by both institutions. However, due to
the COVID-19 pandemic, cooperation on academic activities cannot yet be started.
School’s Information
Princess Chulabhorn Science High School Satun
138 Moo. 12 Chalung, Mueang, Satun 91140
Website : https://www.pccst.ac.th/
Sendai KOSEN 4-16-1 Ayashi-Chuo, Aobaku (5,398.03 km)
9893128 Sendai, Miyagi, Japan
Website : https://https://www.sendai-nct.ac.jp

53

Academic Cooperation between Kumamoto KOSEN
and Princess Chulabhorn Science High School Loei

Since 2016, Office of Basic Education Commission had signed MOU with National
Institute of Technology (KOSEN) to promote academic cooperation on exchange of
students, teachers, academic information and materials, joint research and symposia
o n d eveloping of engineering and innovative mind for junior high school students.
In 2018, Kumamoto KOSEN was invited to participate in the Thailand-Japan
Students Science Fair 2018 in Phitsanulok and PCSHS Loei met teachers and students
from Kumamoto KOSEN for the first time. The cooperation at this event was
very fruitful and beneficial to the development of students in various aspects
including enhancing scientific mind and competences with better interpersonal and
communication skills as well as broadening their global outlook.

In 2019, at the Thailand – Japan Student ICT Fair in Mukdahan, PCSHS Loei had
another opportunity in inviting Kumamoto KOSEN to participate in the Thailand-Japan
Student ICT Fair in Mukdahan. Students had a great time and friendship to get to
know each other. At this event, Kumamoto KOSEN and PCSHS Loei discussed about
an MOU for academic cooperation. The signing ceremony of the MOU was held
online on 18th February, 2021.

School’s Information
Princess Chulabhorn Science High School Loei
129 M.5 That Sub-district, Chiangkhan District, Loei 42110
Website : http://pcshsloei.ac.th

Kumamoto KOSEN
Kumaoto Campus, 2659-2 Suya, Koshi, Kumamoto, 861-1102 Japan
Yatsushiro Campus, 2627 Hirayama-shinmachi, Yatsushiro, Kumamoto, 866-8501
Japan Post code: 861-1102 Telephone: +81-96-242-3798
Website : https://kumamoto-nct.ac.jp/th

54

Academic Cooperation between Akashi KOSEN
and Princess Chulabhorn Science High School Phathum Thani

The academic cooperation between PCSHS Pathum Thani and Akashi KOSEN
started in March 20 when the MOU was signed at Akashi KOSEN for developing
academic cooperation in science, engineering and technology knowledge for students
and teachers. The cooperation agreement is covered: exchange of students, lecturers,
and faculty members; co-operative research activities, exchange of information and
materials in those fields which are of interest to both institutions; or other activities
agreed upon by both institutions.
School’s Information
Princess Chulabhorn Science High School Pathum Thani
51 Village Number 6 Bor-ngoen Sub-district, Ladlumkaew District,
Pathum Thani 12140
Website : https://www.pccp.ac.th
Akashi KOSEN
679-3 Nishioka, Uozumi-cho, Akashi, Hyogo, 674-8501 JAPAN.
Website : http://www.akashi.ac.jp/english/kosen.html

55

Academic Cooperation between Akashi KOSEN
and Princess Chulabhorn Science High School Trang

On February 19, 2019, the MOU for academic cooperation was signed at Akashi
KOSEN by Dr.Hideaki Kasai and Dr. Suwanee Unungwarakorn for developing
academic cooperation in science, engineering and technology knowledge for students
and teachers. The cooperation agreement is covered: exchange of students, lecturers,
and faculty members; co-operative research activities, exchange of information and
materials in those fields which are of interest to both institutions; or other activities
agreed upon by both institutions.
School’s Information
Princess Chulabhorn Science High School Trang
196 Village Number 4, Trang-Sikao Road, Bang-rak Sub-district, Muang District,
Trang 92000
Website : https://www.pcshstrg.ac.th
Akashi KOSEN
679-3 Nishioka, Uozumi-cho, Akashi, Hyogo, 674-8501 JAPAN.
Website : http://www.akashi.ac.jp/english/kosen.html

56

Academic Cooperation between Hachinohe KOSEN
and Princess Chulabhorn Science High School Chonburi

The academic cooperation between PCSHS Chonburi and Hachinohe KOSEN
started on 20 November 2018 with signing of the MOU at Hachimohe KOSEN by
Dr. Wittaya Arunsaengchan, Principal of PCSHS Chonburi, and Dr. Shigenao
Maruyama,President of Hachinohe KOSEN. The aims of the cooperation are to
promote academic exchange and cooperative education and research, focused on
exchange students, exchange teachers, academic staff and administrative staff and
researchers, exchange of academic materials, publications and information and also
joint research and symposia.

Due to the COVID-19 pandemic, we cannot visit each other school, but our academic
cooperation still goes on online. In academic year 2021, we discussed and planned our
academic program through working on Online Workshop, with the topic of introduction
of students of both schools who would like to do the science research together.

All in all, the collaboration program between both schools provided our students
and teachers to gain precious experiences via the scientific research and cultures.
These experiences motivate students and teachers to conduct scientific research that
benefits both themselves and others.

School’s Information
Princess Chulabhorn Science High School Chonburi
695 Moo3, Banbueng-Klaeng Rd., Nongchak Sub-District, Banbueng District,
Chonburi 20170
Website : https://www.pccchon.ac.th/

Hachinohe KOSEN
16-1 Uwanotai, Tamonoki, Hachinohe City, Aomori Prefecture, Japan 039-1192
Website : http://www.hachinohe-ct.ac.jp

57

Academic Cooperation between Ibaraki KOSEN
and Princess Chulabhorn Science High School Phetchaburi

In 2021, 17 representative students, grade 12, from PCSHS Phetchaburi and
10 students from Ibaraki KOSEN participated in “The 1st Ibaraki KOSEN- PCSHS
Phetchaburi Student Conference 2021”. It was held by using an online platform dated
October 11th, 2021. National Institute of Technology (KOSEN), Ibaraki College
and Princess Chulabhorn Science High School Phetchaburi conclude following
understanding for academic exchange and cooperation between parties. This MOU is
based on the idea of agreement on academic exchange both institutions agrees. The
parties agree to develop the activities in areas of education and research of mutual
interest through the followings; exchange of students, teachers and staffs, exchange of
academic information and materials, class activity to enhance communication skills
for students using internet, joint research project with mutual visit of students and
communication using internet, hosting the students, teachers, staffs to do individual
projects in each party, and the program specification such as the number of participating
students, teachers, staffs, the length of the periods, and the content of the program
will be determined through mutual consultations between the parties.
School’s Information
Princess Chulabhorn Science High School Phetchaburi
Khao Yai, Cha-am District, Phetchaburi 76120, Thailand
Website : https://www.pccphet.ac.th
Ibaraki KOSEN
866 Nakane, Hitachinaka-shi, Ibaraki-ken 312-8508 Japan
Website : https://www.ibaraki-ct.ac.jp

58

Academic Cooperation between Nagaoka KOSEN
and Princess Chulabhorn Science High School Nakhon Si Thammarat

The academic cooperation between PCSHS Nakhon Si Thammarat and Nagaoka
KOSEN started in 2019 with the aims focused on academic exchange and coopera-
tive education and research. On May 19th -21st 2019, President Dr Motomu Takeshi-
ke and 2 teachers with 2 students from Nagaoka KOSEN visited PCSHS NST and
participated in academic activities. The MOU was on May 21st, 2019.
On September 2nd -5th 2019, two teachers and ten students from Nagaoka KOSEN
joined the academic exchange activity at PCSHS NST. Students from both schools
joined robotic lectures by the professor from our mentor university. They also learned
to produce some local products in Nakhon Si Thammarat.
On 19th-26th January 2020, ten students with 2 teachers from PCSHS NST
went to Nagaoka KOSEN. The students met the president, had a campus tour and
participated in English and computer (AI) classes. They also visited industrial
factories and city tours. The relationship will take a significant role in the building
of a strong bond between Thailand and Japan.
School’s Information
Princess Chulabhorn Science High School Nakhon Si Thammarat
120 Moo 1 Sun-a-nant Road, Bangjak Sub-District, Muang District,
Nakhon Si Thammarat 80330
Website : https://www.pccnst.ac.th/
Nagaoka KOSEN
888 Nishikatakai-machi, Nagaoka, Niigata 940-8532, Japan
Website : http://www.nagaoka-ct.ac.jp/

59

Appendix III Co-Research Projects
between Super Science High Schools
and Princess Chulabhorn Science High Schools

60

PCSHS Phetchaburi with Bunkyo Gakuin University Girls’ High School
Year 2014 : 1. Comparing the Taste and Constituents of Thai Rice and
Japanese Rice
2. Content of Gamma Aminobutyric Acid (GABA) in Selected
Japanese and Thai Rice.
Year 2015 : 1. Does music affect the increase in GABA?
2. Physical Properties Characterization of Pre-gel Flour from
Japanese and Thai Rice.
Year 2017-2018 : 1. Extraction and Analysis of Amino Acids in Green Robusta
and Arabica Coffee Beans
Year 2018-2019 : 1. Polyphenol Extraction from Thai and Japanese Green Tea

61

Comparing the Taste and Constituents
of Thai Rice and Japanese Rice

Sarocha Suevaja1, Chanoknan Nontakaew1, Sastra Prom-arak1, Apichart Vanavichit2
Ayame Ohashi3, Mika Kanazu3, Ikumi Asai3, Kenjiro Sugiyama4

1Princess Chulabhorn Science High School Phetchaburi, Cha-Am, Phetchaburi, Thailand
2Director of Rice Science Center & Rice Gene Discovery Unit, Kasetsart University
Kamphaengsean Campus, Nakhon Pathom, Thailand
3Bunkyo Gakuin University Girls’ High School, Tokyo, Japan
4Department of Applied Chemistry, Faculty of Engineering, Kogakuin University,
Tokyo, Japan

Abstract
About Japanese and Thai rice, we did the experiment of taste test, and decided the
ranking of each rice. We divided the experiment, and measured the amount of free
amino acid in Japan, the amount of amylose and tenderness in Thailand. Then, we
compared those results to the ranking of taste test, and put together in this poster up
to now. About Japanese rice, there were little differences among the 3 species, on the
other hand there were some differences among the Thai rice, in point of the kinds of
free amino acid and the amount. We thought that result causes the differences of rice
taste, and we could analyze the tendency of favorite. We will continue our experiment
at Kogakuin University in the future.
Keywords : Thai rice, Japanese rice, Tase

62

Content of Gamma Aminobutyric Acid (GABA)
in Selected Japanese and Thai Rice

Hiroko Tanaka1 Tomoyo Taniguchi1 Ikumi Asai1 Kenjiro Sugiyama2
Artitaya Khlambu3 Patcharaporn Pitakchonlasab3 Manas Sittishocktram3

Nattaporn Klinput3 Amonrujee Nakphlaiphan4 and Thanit Pewnim4
1Bunkyo Gakuin University Girls’ High School, Tokyo, Japan

2Department of Applied Chemistry, Faculty of Engineering, Kogakuin University, Tokyo, Japan
3Princess Chulabhorn Science High School Phetchaburi, Cha-Am, Phetchaburi, Thailand

4Department of Chemistry, Faculty of Science, Silpakorn University,
Nakhon Pathom, Thailand

Abstract

ɤ-aminobutyric acid (GABA) produced in organisms from glutamic acid by a bi-
ochemical reaction catalyzed by glutamate decarboxylase. The function of GABA in
promoting good health is well established, thus prompting widespread attempts to
search for foods rich in GABA. We have examined GABA contents in selected Jap-
anese and Thai rice that were subjected to different types of treatments. It was found
that even though normal rice do contain substantial amount of GABA, germinated
rice had higher GABA levels. Germinated Koshihikari rice had about 1.50 folds high-
er level of GABA over the normal Koshihikari rice. The levels of GABA did increase
even further when germination was done under anaerobic condition. The anaerobic
germinated Hom Mali Doi had 2.31 folds higher level of GABA than the aerobic ger-
minated one. The anaerobic germinated Purple Sticky Rice had higher level of GABA
over the aerobic germinated one by 1.47 folds.

Keywords : ɤ-aminobutyric acid (GABA), germinated rice

63

Does music affect the increase in GABA?

Yurika Iwai1 Anna Hanabusa1 Kaede Hosokai1 Suzuka Mizutani1
Ikumi Asai1 Kenjiro Sugiyama2

Chorpaka Nuanyaisithong3 Jurarat Sawangpan3 Manas Sittishocktram3
Amonrujee Nakphlaiphan4 and Thanit Pewnim4

1 Bunkyo Gakuin University Girls’ High School, Tokyo, Japan
2Department of Applied Chemistry, Faculty of Engineering, Kogakuin University, Tokyo, Japan
1Princess Chulabhorn Science High School Phetchaburi, Cha-Am, Phetchaburi, Thailand
2Department of Chemistry, Faculty of Science, Silpakorn University, NakhonPathom, Thailand

Abstract
Germinated brown rice is an innovation that is gaining much attention because it
is a process to increase nutritional value of rice. Normally, rice contains many nutrients
and when brown rice soaked in water to germinate biochemical changes will take place.
Water penetrates the grain will stimulate the enzymes within the grain. Nutrients are
stored in the grain are decomposed by biochemical processes resulted in the formation
of substances such as oligosaccharide and reducing sugar. Moreover, proteins within
the grain are digested to form amino acids and peptides including bioactive compounds,
such as gamma-oryzanol, tocopherol, tri-tocopherol and especially gamma-amino but
triacid, which is also known as “ GABA “. GABA is a neurotransmitter found in the
central nervous system and has important functions in the brain. Recently, many studies
have been reported that GABA provides beneficial effects for human health such as
decreasing blood pressure, helping the recovery from alcohol-related symptoms and
controlling stress. The production of GABA in commercial jasmine rice has been
widely studied, but only few studies reported on the effect of music which actually
consists of sound waves to the amount of these substances in local germinated brown
rice. So in the present study, the amount of GABA in local germinated brown rice and
after musical stimulation in germination process were investigated.
Keywords : ɤ-aminobutyric acid (GABA), germinated rice, music

64

Physical Properties Characterization
of Pre-gel Flour from Japanese and Thai Rice
Santisuk Kansuwan1 Montra Patumasut1 Manas Sittishocktram1

Nattaporn Klinput1 Thanit Pewnim2
1Princess Chulabhorn Science High School Phetchaburi, Cha-Am, Phetchaburi, Thailand
2Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom,

Thailand

Abstract
This project aims to compare the physical properties of normally flour and
pregelatinized flour (Pre-gel flour) from Japanese (Akitakomachi and Koshihikari) and
Thai (HomNil and SangYod) rice. The moisture, swelling power and peak viscosity
were determined and comparison. The results indicated that the moisture content of
all normally and Pre-gel flour were below 13% which were standard for using in food.
The temperature about 55oC-65oC was the best temperature for Pre-gel flour swollen
but normally flour. Swollen at higher than 75oC. Pre-gel flour from SangYod and
Akitakomachi rice showed the highest swelling power about 1476.67 and 1334.00 g/g
of Thai and Japanese rice, respectively. This indicated to the molecule of flour
would be disperse and untidy lead to absorption. So the flour two kinds of nice were
appropriate for making best quality of bakery and other.
Keywords : Pre-gel flour, swelling power

65

Extraction and Analysis of Amino Acids
in Green Robusta and Arabica Coffee Beans

Siriganda Teingtung Saowarot Meeneam
Advisor : Sastra Prom-arak

Princess Chulabhorn Science High School Phetchaburi, Cha-Am,
Phetchaburi, Thailand

Abstract

In Thailand, there are two coffee varieties, coffee Arabica and coffee Robusta. In
the process of producing coffee, green coffee beans are heat roasted. Many substances
are lost when they are heated, especially protein and amino acids as other forms of
Protein. The project developer is interested in finding out how to extract protein in
green coffee beans to obtain the highest protein content. The purpose is to analyze the
significant quantities of green coffee beans from Robusta and Arabica, extracted by
different methods. The experiment was carried out with the green coffee beans peeled
and baked in a hot air oven at 40°C for 48 hours. The experimental group was divided
into 5 groups, submersed in distilled water, immersed in 0.1 M HCl solution, immersed
in 0.1 M NaCl, 70% ethanol, boiled with distilled water at 60°C for 60 minutes. Then
all were shaken. At a speed of 120 rpm for 60 minutes. Next all were shaken at 120
rpm. There were 13 amino acids that had a higher, extraction rate than that of other
methods. The green coffee extract of Robusta was immersed in distilled water and
shaken at 120 rpm for 60 min. This method extracted 11 amino acids at a higher rate
than other methods. This extraction method can be applied in the processing of coffee
beans.

Keywords : Arabica, Robusta, green coffee beans, amino acids

66

Polyphenol Extraction from Thai and Japanese Green Tea

Monsicha Yorddamnurnkun1 , Thananya Phupha1 , Thatsaneeya Matukarak2 ,
Miho Kudo2 , Haruka Yanagisawa2

1Princess Chulabhorn Science High School Phetchaburi, Cha-Am, Phetchaburi, Thailand
2Bunkyo Gakuin University Girls’ High School, Tokyo, Japan

Abstract
Green tea is the most widely consumed beverage in the world. The consumption is
widespread due to its aroma, taste, beneficial effects and also highly nutritional which
is Polyphenols. It is an antioxidant that can be found in Cocoa, wine and in particular
green tea. This project studied the amount of Polyphenols in Thai and Japanese green tea
which are separately extracted in raw and processed states of both kinds with methanol
and brewed with distilled water in 60˚C and 80˚C by Folin-Ciocalteu’s phenol
reagent process and finding absorbance at 767.5 nm. The results, the amount of
Polyphenol in raw Thai and Japanese tea leaves which extracted with methanol was
120.359 and 1772.728 µg/mL respectively. On the other side, the amount of Polyphenol
in processed Thai and Japanese tea leaves which extracted with methanol was 688.716
and 414.003 µg/mL respectively. In another experiment, finding amount of Polyphenol
by brewing both Thai and Japanese Processed tea with distilled water in 60˚C and
80˚C degrees. The result, at 80˚C, the amount of Polyphenols in green tea was higher
than 60˚C, both Thai and Japanese green tea. Then, Thai and Japanese green tea are
compared by t – test independent that showed the amount of Polyphenol form both
experiments, there was significance difference. Different amounts of polyphenols of
dried and fresh green tea due to different fermentation and heat processes of tea drying.
There are also other factors, such as the type of tea, planting method and the different
environment that affect to different amounts of Polyphenols in tea.

Keywords : green tea, polyphenol

67

PCSHS Loei with Shizuoka Kita Junior and Senior High School
Year 2017 : A study of the diversity of freshwater fish and the physiology
of some freshwater fish in Thailand : case study of Mekong fish in Chiang Khan
District, Loei Province
Year 2018 : 1. A study of natural disaster and planning to cope with
the earthquake and Tsunami in Shizuoka.
2. A study of natural disaster and planning to cope with
the land slide in Loei.
Year 2019 : 1. A study of a simple water purification technique.
2. A study of the diversity in Loei river Thailand

68

A Study of the Diversity of freshwater fish and the physiology of some
freshwater fish in Thailand: case study of Mekong fish
in Chiang khan District, Loei province.

Ketklao Thanasoot1, Autchariya Suksomboon1, Supapich Wongpanya1, Chaiwat
Khampamuan1, Ariyaporn Sudkaew1, Jakkree Somwong1, Sukhita Phuchathong1

Hiroyasu Nakano2, Hatsuki Shirai2, Shuhei Ogawa2
Advisor : Weerayut Supiwong3

1Princess Chulabhorn Science High School Loei
2Shizuoka Kita Junior and Senior High School

3Khon Kaen University

Abstract
The objectives of this study are
1. To survey the fish diversity in the river at Mekong River.
2. To study about the principles of classification and identification of freshwater fish.
3. To study the chromosome preparation in fish and determine the chromosome
diversity in freshwater fish in Thailand.
Twenty kinds of fish were observed to identify of species following by the hand
guidebook ‘Rainboth 1996’ by measuring the scale, the morphology, shape, size, body
plan, the character, length, number and modify of fin. A Chromosome diversity of
freshwater fish was conducted by cell division inhibition at metaphase step to count
chromosome of each fish by using a tissue of kidney. Twenty figures of sample were
count for the number of chromosomes to confirm the diploid number which is the
most frequency of the number found is 2n in that species.

Keywords : Mekong fish, Biodiversity

69

A study of a simple water purification technique.
Thanawit Thongsaen1, Phachara Makarajaroenkul1, Peeraphat Thanasoot1,

Nattaphon Naewtan1, Nanthikan Maraming1, Siriyakorn Theprat1
Shunki Nayasaka2, Shiki Iwamoto2, Yuki Sasaki2
1Princess Chulabhorn Science High School Loei
2Shizuoka Kita Junior and Senior High School

Abstract
Water is necessary for the lives of humans, animals, and plants. This often uses
water from groundwater or surface water. From natural changes and human activities
may cause water to have suspended solids, turbidity, or residue in water. Therefore,
we have guidelines for filtering water from groundwater or surface water that contains
suspended solids, turbidity, and sediment to become clean water that can be used for
consumption. By making water filters from natural materials that can be found locally.
From the idea of making water filters, we have the idea to choose a variety of natural
materials such as volcanic soil, sand, charcoal, and rock to study which materials can be
mixed for the best filter.
The result of this project shows the filters consisting of sand, charcoal and rock can
filter the water to get the cleanest water.
Keywords : water filters, groundwater, natural materials

70

A Study of the Diversity of Aquatic animals in Loei River, Thailand.
Pimpaporn Sunatho1, Pimchanok Khonkwang1, Pichamon Thaibpet1, Naphasakit Chaiyota1

Ryuki Tonooka2, Kyosuke Miyata2
Advisor : Aniwat Pengsawang3

1Princess Chulabhorn Science High School Loei
2Shizuoka Kita Junior and Senior High School
3National Science and Technology Development Agency

Abstract
Aquatic animals that live in any area can indicate the quality of the water in the
area. Therefore, the purpose of this project is to study small aquatic animals in the
Ban Kaeng Mi which is a water source area, to assess the water quality from the ob-
servation of the aquatic animals, and to identify whether each aquatic animal indicates
the quality of the water as clean or dirty. The River Detective manual was used to be
a reference methodology for this study. The study concluded that the water source in
the Ban Kaeng Mi area has the ideal water quality for the livelihoods of animals and
aquatic plants.
Keywords : Aquatic animals, biodiversity, River detective.

71

PCSHS Pathum Thani with Tennoji Senior High School attached to
Osaka Kyoiku University

Year 2018 : 1. Physical properties of bread-crumb and the study sand
comparison of young’s moduli, case study

2. The study relationship between angle from x axis, slope of the line
and coordinate ( x,y ) of r-unit – radius circle
3. A comparative study on ability to maintain condition and inhibit
the contamination of fungus on fruit that is coated with abelmoschus
Esculentusokro(okra) gel and extract from cassia fistula (golden
rain tree)
4. The application of nuclear technique for measuring volume of
potassium, thorium and uranium in rice field’s soil.
5. Developing system for recording student activities with Ionic
framework 4 for teacher and student of Princess Chulabhorn
Science High School Pathumthani.
6. A comparison of efficiency of heat insulation produced from water
hyacinth fibers and jute fibers by using natural rubber as binder.

Year 2019 : 1. Nursery bag from rice stubble, tapioca starch and natural rubber
2. Comparison about effective of Thai herb and Japanese herb to
decrease stress
3. Study of the effects of the ratio between mass’ weight and string’s
length in Simple Harmonic pendulum
4. Oil filter sheet from natural material as a wastewater treatment
5. Gas and power outage alarm system (ICT fair 2019)
6. Thai foods calories scanner application with object detection (ICT
fair 2019)

72

Gas and Power Outage Alarm System
Napat Sirijaitam1 , Panchita Meksakulwong1

Ayana Hata2, Moeka Wada2
Advisor : Thanabath Kongkaew1, Pracha Khamphakdi2
1Princess Chulabhorn Science High School Pathum Thani
2Tennoji Senior High School attached to Osaka Kyoiku University

Abstract
The objective is to detect LPG gas, Natural Gas and alert when voltage has
dropped by module Mq-5 and Single-Phase Voltage Sensor Module AC 220V notify
via WiFi, we found that the program able to detect when have LPG Gas leak or Voltage
dropped and notify. That useful when have not any people or have only child and
elderly person at home. That can detect gas and power outage to alarm.
Keyword : Arduino, module, programming language

73

Thai & Japanese Foods Calories Scanner Application with Object Detection
Chayangoorn Sonsena1

Kotaro Kanazawa2, Yuma Bono2
Advisor : Thirakan Pakorn Sanso1
1Princess Chulabhorn Science High School Pathum Thani
2 Tennoji Senior High School attached to Osaka Kyoiku University

Abstract
The objective is to develop applications to measure calories of Thai food from
pictures and evaluate the accuracy of the model. This application can tell the calories
of food from pictures. Either from taking one or several foods at a time, and notifying
when the calories in that day exceed the TDEE (Total Daily Energy Expenditure).
Development parts divided into two parts:
1) Model development using Object Detection system from Google AutoML
2) Application development using Ionic Framework 4 which will benefit those who
want to control weight. They which need to see the amounts of calories eating each
day. Planning the next meal or the next day when the model has been completed, the
accuracy of the model is 85%.
Keyword : Deep Learning, Application, Object Detection, TDEE

74

PCSHS Pathum Thani with Ritsumeikan Keisho Junior
and Senior High School

Year 2017 : 1. Indio dying
2. Monk fruit
3. Water filter from bio-fiber
4. The way which can drink tap water in Thai
5. Bio organism related to acidification

Year 2018 : 1. Developments of paper-based microfluidics analytical devices for
Phenolic compound and Antioxidants Potential in fruit Beverages
2. Monk Fruit (Siraitia grosvenorii) Sweetener
3. The study of the effect of the trigonometric pattern of the music
chords on human relaxation: Electroencephalogram (EEG)
4. Jelly adding Paracetamol Syrup for Children
5. Transfiguration Shockproof Material made from Pineapple Leaves
and Hydrogel
6. Development of low fat coconut milk from konjac powder and soy
protein milk

Year 2019 : 1. CEFR words learning game (ICT fair 2019)
2. The study of produced sound from bamboo’s tube. (TJSSF 2020
Online)
3. The study of antibacterial activity of radish against Staphylococcus
aureus.
4. The study of produced sound from bamboo’s cylindral tube.
5. The studies of anti-fungal Activity of Scallop Shell Powder against
Aspergillus Niger
6. The Comparison of Properties between Thai and Japan Nepenthes

Year 2020 : Online
1. Optimal Pretreatment of Rice Bran as Plasticiser in Making
Flexible Bioplastic
2. The repeatedly heated oil

75

The study of produced sound from Bamboo’s Tube
Thunchanok Watthapornsiri1, Pimmada Sawangwong1,

Yuika Sakaguchi2, Yukino Watanabe2,Minori Ishii2, Kotaro Meguro3
1Princess Chulabhorn Science High School Pathum Thani
2Ritsumeikan Keisho Senior High School,
3Sapporo Minami High School

Abstract
In this experiment, researchers studied the characteristics and properties of sound
produced from bamboo tubes. For this experiment processed by preparing bamboo
cylindrical tubes of different lengths, radius, and hole’s position in reference to the
tube’s mouth have been prepared. Then 10 N of force was applied to the tube’s mouth by
using a metal rod. After that, the sound was recorded and analyzed using “Audacity”.
The first experiment is used to establish the relationship between sound produced and
length, 15, 20, 25, 30 cm. The length of the tubes was investigated. The result shows
that the longer the tube, the lower the frequency, causing the higher the period. For
the second experiment concerning the hole’s position, a hole was drilled 5, 10, 15, 20,
25 cm away from the tube’s mouth. The result shows that the further the hole, the
higher the frequency, and the lower the period. In the last experiment, the radius is
observed. The result shows that the bigger the tube, the lower the frequency, and the
higher the period.
Keywords : Bamboo, Sound frequency, Period

76

The Designing And Developing Of English Learning Game
Based on CEFR Standard.

Isaku Kawanishi1, Sakura Watanabe2, Methawee Konkangplu3, Sornsiri Phathanaprasitchai3
Adviser : Mogi Haruki3, Takayuki Fukuda1, Chutharat Chai-ngam3
1 Ritsumeikan Keisho Senior High School
2Sapporo Kaisei Secondary School
3Princess Chulabhorn Science High School Pathum Thani

Abstract

English language will be more important and people want to be able to speak
English fluently. However, it’s difficult for a foreigner to learn English language by
themselves. Thus, we may find a new way to learn English easier. A game could play
a major role in enhancing and enriching learning for the player since it helped the
player to concentrate on their given task. Accordingly, applying a game on education
would mean interest in learning. Therefore, we have an idea to practice English
anywhere and anytime using game. Our objective is to create a game that help the
player to learn English words by developing their recognition skill. In this game,
CEFR standard was used to divide player’s language ability. There is CEFR word
learning game which is media for education. This software uses vocabulary, helping
the player to recognize the vocabulary easily.

Then play the game by matching the word with the correct picture. C# language
were used to create in-game function on Unity platform. The project results will also
promote the inclusion of serious games as one of the digital resources with educational
opportunities. Promoting its study by future teachers as a reliable tool that their own
students can experience and from which they can learn safely. In each CEFR standard
level, 30 high school students were tried out. The result implied that they enjoy learning
new words with satisfaction level of Good. For further works, this game could be
developed to an online system for collaborative learning.

Keywords : CEFR, words learning game, Unity, C#

77

Transfiguration Shockproof Material made from Hydrogel
and Pineapple Leaves Cellulose

Sawitri Prompinit1, ApichayaThongrat1, Kikuta Daiki3, Rokkaku Natsuki3
Advisors : Chutharat Chai-ngam1, FUKUDA Tayayuki2
1 Princess Chulabhorn Science High School Pathumthani,
2Ritsumeikan Keisho Senior High Scool
3Sapporo Kaisei Secondary School

Abstract

Online spending on products and services by Thai people is likely to increase to
376.8 billion baht in 2017 due to the transportation of goods to the destination. But in
transportation, there are disposable cushioning materials Causing a large amount of
degrading waste. Therefore, the authors aim to study the ratio of hydrogel to cellulose
that affects the forming of cushioning material. By using glutamine aldehyde as a
crosslink between chains in order to obtain a hydrogel with a three-dimensional
mesh structure and extract cellulose from pineapple leaves with a sodium hydroxide
solution for reinforcement. The ratios are 10%, 15% and 20% w/w using cellulose,
respectively. From the experiment, it was found that the ratio of 15% w / w cellulose
can be molded. There is a constant distribution of cellulose compared to the amount
of hydrogel. The resistance to tensile strength which affects the shape and strength
of the cushioning material has been studied too. A tensile strength is 0.4836 MPa.
Shock resistance has been observed, which affects the transmission of impact force to
be passed to the product to decrease with resistance to shock resistance 4.8x10-4 J/cm2,
reduce loss and damage of products. Furthermore, the shape changes have been studied
with a variety of styles regarding the ability of the product to absorb water of the
hydrogel. An average of 55% water absorption has been found, which is the result of
water molecules inserted into the space between the chains causing swelling when
water is absorbed and shrink when losing water. This causes the cushioning material
to change its shape Save storage space, suitable to be used as a cushioning

Keywords : Shockproof Material, Hydrogel, Cellulose, Crosslink, Transfiguration

78

Development of low fat coconut milk from Konjac powder and soy milk

Hosokawa Hana2, Maeda Risa2, Atthawadee Kharabun1, Sasitorn Thanapongpipat1,
Bhornthamarin Sukchuen1

Advisors : Chutharat Chai-ngam1, Fukuda Tayayuki2
1 Princess Chulabhorn Science High School, Pathumthani

2Ritsumeikan Keisho senior High Scool

Abstract
In cooking, coconut milk is often used as an ingredient. Unfortunately, obese
patients cannot consume foods made from coconut milk. Because doctors have
determined the amount of fat that the patient can consume up to 30 percent of the energy.
There are two parts in coconut milk: coconut milk and coconut milk, 93 percent
of the energy in coconut milk is derived from fatty foods. This project therefore aims to
develop coconut milk using konjac powder and soy milk as a fat substitute in coconut
milk. In this research, it was found that the konjac powder obtained from dried
extraction had a viscosity of about 15,000 centimeters and soy milk was colloidal
liquid. When mixing both pairs together to form a homogeneous liquid, similar to
coconut milk which will be measured properties to compare the amount of fat in
coconut milk. Bringing coconut milk to cook fishy dishes such as the green curry and
sweet food, named Chalong, in sensory test compared to the general coconut milk
in the experiment, a comparison of the ratio between konjac powder and soy milk is
1: 50, 1: 25 and 3: 50 mL. To find a suitable rate for developing modified low-fat
coconut milk based on the study of the ratio between konjac powder and soy milk, it
is known that when the amount of konjac powder is less than soy milk, the coconut
milk will be similar to coconut milk in the market regarding the color and texture.
Keywords : coconut milk, konjac powder, soy milk, development

79

Development of microfluidic Paper based analytical device for phenolic
compounds in fruit beverages

Omote Ayuki3, Nakagawa Risa1, Chanuntorn Deeprasert1, Araya Phalang1
Advisors : Petcharee Ruksasri1, Chutarat Chai-ngam1, Takayuki Fukuda2

1Princess Chulabhorn’s Science High School Pathum Thani
2Ritsumeikan Keisho Senior High School

3 Sapporo Intercultural and Technological High School

Abstract
This experiment developed a paper-based measuring device that was created
using a screen-printing method with a resin that has hydrophobic properties is the
condition of the substance that water cannot penetrate, therefore, it is insoluble in
water. In order to determine the extent of the reaction to be easier to analyze results,
the paper-based portable devices was eveloped and the phenolic compounds and
antioxidant were measured by reacting with methods Folin-ciocalteu Down to the
number of paper measuring devices 3 times, then take a picture and then study the
color intensity from the analysis with Image J program and create a graph standard
Gallic acid to compare the concentration of phenolic compounds in samples. The
results show that sample 1 has a concentration of phenolic compounds 1.351 GAE
mg/L. and sample 2 has a concentration of phenolic compounds 0.205 GAE mg / L
and sample 3 has a concentration of phenolic compounds. Alice 0.209 GAE mg / L
Can create paper measuring devices with screen printing methods with resins and
can create a standardized graph (Gallic acid) that is used in comparison to determine
the concentration of phenolic compounds in various fruit juices and confirmed
results by finding% recovery, which in this experiment is in the range of 88% - 104%.
The method that uses paper-based devices developed in this experiment is a convenient
and easier way than testing Find phenolic compounds.
Keywords : Microfluidic paper based, Phenolic compounds, Fruit beverages

80

The Study of the effect of the pattern of Japanese and Thai music on human
relaxation : Electroencephalogram(EEG)

T-touch Pattaravarodom1, Juntrarat Jun-a-nun1, Ohashi Rei2, Shibata Aya3 and Todo hikari4
Advisors : Chaweewan Arunthaworn1, Sunisa Kongkalai1, Somyote Noipailom1,
Chutarat Chai-ngam1, Takayuki Fukuda2
1Princess Chulabhorn’s Science High School Pathum Thani
2Ritsumeikan Keisho Senior High School
3Sapporo Kaisei Secondary School
4Sapporo Intercultural and Technological High School

Abstract

Music is one thing that makes people feel a lot of emotions. In the past since the
Pythagorean era until now, there are so many researches in mathematics and biology
that related to music. So we would like to study the relationship between trigono-
metric patterns of music chords, study and compare relationships between the bar of
songs along with comparing the changes of the brainwave that indicate the relaxa-
tion of music. And compare the characteristics of each ethnic music, in the form of
mathematics and biology. The music that will be used in this experiment cause both
relaxations, that is Pornpeemai and Mouikutsuneruto Oshougatsu

The results show that the pattern as a period of the intersection of the frequency
of each note in various music chords from using the GeoGebra program. So, the Ma-
jor chords will occur the period of the intersection of the frequency of the 3 notes in
the chord at the same point with a wavelength of 2, 2.5, 3 periods, respectively. The
pattern of the Minor chords also creates the intersection from all 3 wavelengths at the
same point as well. With a wavelength of 8, 9.5, 12 periods, respectively. It also found
that there are patterns of chords in each bar in the form of music and mathematics.
For comparing the changes of the brainwave that indicate the relaxation by using
the EMOTIV Insight 5 Channel Mobile EEG to record the changes while listening
to music from a sample group of students from Princess Chulabhorn Science High
School Pathum Thani for 10 people and students from Japanese high school for 10
people. Assuming that there will be a change of relaxation while students from each
country listening to their homeland song as much as possible.

Keyword : Music chord, Trigonometric pattern, Brainwave, Human Relaxation

81

Monk Fruit (Luo Han Guo) sweetener

Nutthanon Kandet1, Pimmada Pitaksahapoom1, Eriko Horiguchi4, Rio Kitamura3
Advisors : Chutharat Chai-ngam1 and Takayuki Fukuda2
1Princess Chulabhorn Sceince High School Pathumthani
2Ritsumeikan Keisho Senior High School

3Sapporo Kaisei Secondary School 4Sapporo Minami High School

Abstract

The fruit of Luo Han Guo or Monk Fruit (Siraitia grosvenorii) contains the herbal
and non-caloric sweetener called “Mogrosides” which give 250 times the sweetness of
sugar. In this experiment, we have the focusing objectives of 1)to extract the sweetener
from Luo Han Guo with different methods and test the properties of the obtained
substances, and 2)to apply to food products as both traditional food, Thailand and
Japan, to do a sensory experiment using 9-Point Hedonic Scale. We invested 2 ways
of extraction, the first method takes 1 hour to boil the fruit in 100 ºC and another
take 3 minutes with the same process. As a result, we could extract 4.58 %Brix and
4.17 %Brix of the sweetener in order of the first and latter method.

The colorimeter was used to identify the brown color of both crude extracts. The
results of L*, a*, and b* were identified as 11.95, -9.07, and 0.58 for the first, and 11.71, -9.47,
and 0.35 for later. After the calculation, we came out with the replacement ratio of 1 gram
of sugar and 1 milliliter of our extracts. In the sensory experiment, we firstly decided
to make jelly and milk tea as a Thai traditional food products. After we run the satisfaction
test out of 9 points with the sample, the first method Luo Han Guo jelly got 5.68±0.69,
sugar jelly with 7.40±0.53 and second method Luo Han Guo jelly with 5.48±0.69. The
first, sugar and second recipes of milk tea also got 7.08±0.45, 7.32±0.33 and 7.48±0.39
in order. The Japanese traditional food called Teriyaki gotfsfsfgegtt. In conclusion, both
extractions could be used to extract the sweetener from the fruit, although the second
method is better with less spending of time. The Luo Han Guo extract can be used to
replace sugar in the food that contains some other substance giving the main taste and
smell of the food. The pure usage in the food containing a lot of sugar as its taste is
not recommended, otherwise, the extract can be used after separating the Mogroside
IIe which gives the taste of bitter. The crude extracts could be used in order to decrease
the amount of sugar in food as well.

Keywords : Non-caloric Sweetener, Monk Fruit (Siraitia Grosvenorii), 9-Point
Hedonic Scale

82

Jelly adding Paracetamol Syrup for Children
Methaporn Suepa1, Chanaporn Zhang1, Watanabe Sakura2, Noguchi Nanaka2, Okuda Ayana2

Advisors : Chutharat Chai-ngam1, Fukuda Tayayuki2
1Princess Chulabhorn Science High School, Pathumthani

2Ritsumeikan Keisho senioir High Scool

Abstract
Paracetamol is a common home remedy for fever but has a bitter taste. Makes
children difficult to eat. So, we conducted the experiment to combine paracetamol with
jelly. To Test the stability of Paracetamol Syrup in jelly. And Study of sugar content
and physical medicine. Then have stability test of physical medicine including pH, Brix,
Color, Moisture values. It was found that the pH value is not significantly different.
From the sweetness test (Brix), it was found that the amount of sugar increased, the
sweetness will increase. From the color value test found that the amount of sugar
increased, the color is darker. From moisture test, found that the amount of sugar
increased, the humidity will decrease. And the best formula is the formula that
contains 14 grams of sugar.
Keywords : Jelly, Paracetamol Syrup, Stability, pH-value

83

The study of antibacterial activity of radish against Staphylococcus aureus.
Parinporn Chairattana-apirom1, Salisa Kunponmuang1,
Kenta Ohwada3, Reiji Ichinohe3, Kouki Toyama4

Advisor : Tichakorn Satjing1, Warangkhana Thuphakdee1,
Chutharat Chai-ngam1, Takayuki Fukuda2

1Princess Chulabhorn Science High School Pathumthani,
2Ritsumeikan Keisho Senior High School,
3Sapporo Moiwa High School,
4Sapporo Kaisei Secondary School

Abstract
Radish (Raphanus sativus L.) is a rich source of chemical compounds like phenols,
alkaloids, glycosides and sterols. These compounds are believed to have potential
components against several pathogenic microbes. In this experiment, researchers
are studying the antibacterial activity of radish against Staphylococcus aureus.
The experiment is divided into 3 steps: The first step is the process of preparing the
radish extract, the second step is the preparation of the Nutrient Agar and the final
step is testing of the antibacterial activity of the radish crude extracts. The result
shows that from 81.99 g of dried radish to 18.79 g of radish extract can be produced,
which corresponds to a 22.92% yield and when the concentration of radish extract is
increased in steps of 10 mg/ml, diameters of the produced clear zones (mm) increase
in size. It’s confirmed that radish has antibacterial compounds.

Keywords : Radish, Staphylococcus aureus, Antibacterial activity, Clear zone

84

Comparison of Properties between Thai and Japan Nepenthes
Tsubasa Nakayama1, Napatsawan Yousuk1, Minori Ishii2,
Kureha Kurokawa3, Hiyu Tanaka4, Yukino Ando5,

Advisors : Chutarat Chai-ngam1, Takayuki Fukuda2, Keita Masuda5
1Princess Chulabhorn Science High School Pathum Thani
2Ritsumeikan Keisho Senior High School
3Sapporo Moiwa High School
4Sapporo Kaisei Secondary School
5Sapporo Minami High School

Abstract
How does the pH value of the digestive liquid of nepenthes vary between
different species? If openings exist, which allow the release of odors to attract insects,
is there any relation between the pH-value and the size of the opening? In order to
answer these questions, three species of nepenthes were studied and compared their
pH-value and the size of their opening by using pH- meter and microscope. Additionally
due to our curiosity, we put some insects into the nepenthes and measured the pH to
know if the value changes or not. From these experiments, it can be concluded that
each species shows different pH-value. Additionally, the age of each peristome has an
effect on the value, the older it is the higher is the value. This indicates that the ability
of digestive liquid is influenced by various factors.
Keywords : Nepenthes, digestive liquid, pH-value, openings to attract insects

85

The studies of anti-fungal Activity of Scallop Shell Powder against
Aspergillus Niger.

Rujapar Wongrat1, Suthumma Orachai1, Sakine Nishi3, Karen Makino4,
Kana Takechi4, Jilaphiya Boonmanang5 and Warapon Munikom5

Advisors : Chutarat Chai-ngam1, Tichakorn Satjing1, WarangkhanaThuphakde1,
Takayuki Fukuda2, Arawan Chaiyachat5,

1Princess Chulabhorn Science High School Pathum Thani
2Ritsumeikan Keisho Senior High School
3Hokkaido Sapporo Minami high school

4International Christian University high School
5Debsirinklongsibsam Pathumthani School
4Sapporo Kaisei Secondary School

Abstract
In this project, researchers have studied the anti-fungal of Scallop Shell Powder
from Japan and Thailand against Aspergillus Niger and to compare the anti-fungal
of Scallop Shell Powder in different concentration against Aspergillus Niger. This
experiment is divided to 2 steps. The first process is the process of preparing scallop
shell powder from Thailand, the second process is comparing the scallop shell
powder from Thailand and Japan by poisoned food technique. As a results, the
100%v/v concentration of Japanese scallop shell powder made Aspergillus Niger
grew in the smallest area, has shown that Japanese scallop shell powder and more
concentration impact more anti-fungal actions.

Keywords : scallop shell powder, Aspergillus Niger, anti-fungal activity

86

Natural Sweetener extracted from Monk Fruit (Siraitia grosvenorii)
Tsubasa Nakayama1, Napatsawan Yousuk1, Minori Ishii2,
Kureha Kurokawa3, Hiyu Tanaka4, Yukino Ando5,

Advisors : Chutarat Chai-ngam1, Takayuki Fukuda2, Keita Masuda5
1Princess Chulabhorn Science High School Pathum Thani
2Ritsumeikan Keisho Senior High School
3Sapporo Moiwa High School
4Sapporo Kaisei Secondary School
5Sapporo Minami High School

Abstract
Monk fruit (Siraitia grosvenorii), also known as Lo Han Guo, is being used to
sweeten foods and beverages. Monk fruit sweetener is created by removing the seeds
and skin of the fruit, crushing the fruit, and collecting the juice. In this project, active
compounds (sweet factor) from Monk fruit by two different methods and measure the
sweetness of crude extract using Brix Refractometer and compared to controls. The
results show that sweetener crude extract can be extracted from Monk Fruit using
DI water as solvent. The extract from the powder is more %Brix value than the small
piece because of having more surface areas.
Keywords : Sweetener, Monk fruit

87

Bio organism related to acidification; case study, Ukiusa (Duckweed)
Ito Hiroki2, Tomizaki Hiroki2, Asami Hinako2, Tanu Seursumrit1, Chatbodn
Yingtaweezittikul1, Punharee Lunvongsa1, Ms.Kanpicha Tubwongsin1
Advisor : Ms.Tichakorn Sadjing1, Mr.Kurosawa Yoh2
1Pricess Chulabhorn science high school Pathumthani,
2International Christian University high school

Abstract
One of the causes of fresh water acidification is acid rain. That means rain has
unusually acidic (low pH). Acid rain has harmful effects on plants and animals. So,
acid rain plays a big role in ecosystem that should be aware. Some Organism has
affected on this harsh condition. Therefore, we want to know what kind of organism
can live in that situation and find its cause that why it can survive. Duckweed can be
found both in Thailand and Japan. So, we designed to use it as a organism sample.
Two acids were used in these experiments as a virtue acid rain; HCl and NaCO3.
varying these acid concentrations and set it by 1-7 pH, then put a Duckweed plant
into it and see how its change and have explored the reason why it had changed.
The results are shown that pH below than 4 is caused Duckweed structure and color
changed.
Keywords : fresh water, acidification, organism, pH,

88

PCSHS Phitsanulok with Seishin Gakuen High School
and Junior High School

Year 2018 : Diversity of Mosses at Tat Duean Waterfall, Si Satchanalai National
Park, Sukhothai Province and Seishin Gakuen High School and the Growth of
Taxithelium Mosses and Hypnum plumaeforme Mosses on Different Substrates
Year 2018 : A Study of Polystyrene Foam Dissolving Efficiency of Eucalyptus
Essential Oil, Lemon Grass Essential Oil and Peppermint Essential Oil
Year 2019 : A Study of Inhibition Properties of Mold Starter Against Pathogenic
Bacteria Found in Citrus Plants

89

Diversity of Mosses at Tat Duean Waterfall, Si Satchanalai National Park,
Sukhothai Province and Seishin Gakuen High School;

and the Growth of Taxithelium Mosses and Hypnum plumaeforme Mosses
on Different Substrates

Natthaya Jandahong1, Pimphavee Jandahong1, Haruna Wakuda2, Kano Takasu2,
Sayaka Mukouyama2

Advisors: Phurit Khongniam1, Hideyuki Jumonji2,
Kanjana Thananoppakun3, Kenichiro Ishida4, Masaki Yoshida4

1Princess Chulabhorn Science High School Phitsanulok
2Seishin Gakuen High School and Junior High School
3Pibulsongkram Rajabhat University 4Tsukuba University

Abstract
The objectives are divided into two sections, Section 1, survey community of
mosses at Tat Duean Waterfall and Seishin Gakuen High School and compare the
diversity of mosses between Tat Duean Waterfall and Seishin Gakuen High School.
Section 2, the growth of Taxithelium mosses in Thailand and Hypnum plumaeforme
mosses in Japan on each substrate. At Tat Duean Waterfall 17 genera were found.
They were divided into two groups, i.e. 6 genera of Acrocapus mosses and 11 genera
of Pleurocapus mosses. Fissidens, Taxithelium and Hyophila respectively were the
most common genera. At Seishin Gakuen High School Liverwort, Brachytheciaceae,
Bryaceae and Fissidentaceae families were found. Mosses in the shade, dome and
well-ventilated area were mainly found. Two families were found in both places. Part
2, the growth of Taxithelium moss in Thailand and Hypnum plumaeforme mosses in
Japan on different substrates, namely granite, sponge, polystyrene foam, plastic and
cotton in six weeks period. The result showed that mosses can grow on all substrates
with different characteristics.
Keywords : moss, Tat Duean Waterfall, Seishin Gakuen

90

A Study of Polystyrene Foam Dissolving Efficiency
of Eucalyptus Essential Oil, Lemon Grass Essential Oil

and Peppermint Essential Oil

Kornkanok Kaeowichian1, Thanaree Prasoetpornsri1, Kaho Yamagiwa2, Naoko Osone2,
Sota Shinotsuka2, Yuichiro Kato2, Kodai Yamamoto2
Advisors: Duangnapa Sompong1, Kiyomi Oroku2,
Kenichiro Ishida3, Masaki Yoshida3

1Princess Chulabhorn Science High School Phitsanulok
2Seishin Gakuen High School and Junior High School

3Tsukuba University

Abstract
Foam is a kind of plastic that is produced by the process in which butane (C4H10)
or pentane (C5H12) is used to increase its volume (Expandable Polystyrene Foam,
EPS). Polystyrene has become widely used in industry; for example, it is used for
packaging or shock proof in goods transportation, because it is light-weighted, cheap
and convenient. However, foam took long time to degrade in nature and incineration
causes pollution problem. Previous research showed that the extract of orange peel has
an active compound called d-limonene which can dissolve polystyrene. Eucalyptus
essential oil, lemon grass essential oil and peppermint essential oil are available on
market. In this study, the efficiency of dissolving polystyrene foam of these tree essential
oils was investigated. The result showed that polystyrene form was much more
dissolved in high temperature than at room temperature (25°C). All selected essential
oils gave the better results than essential oil from orange. Peppermint essential oil
has the best result both in foam dissolving ability and tensile strength. This can be
applied to commercial use because peppermint essential oil has low cost.
Keywords : EPS, polystyrene, orange peel essential oil, eucalyptus essential oil, lemon
grass essential oil and peppermint essential oil

91

A Study of Inhibition Properties of Mold Starter Against Pathogenic
Bacteria Found in Citrus Plants

Ariya Bualuang1, Warangkhana Rotsakan1, Masahiro Suzuki2, Ruri Sugiyama2
Advisors : Taweesak Piumtap1, Hideyuki Jumonji2, Jaruwan Thongsanit Okumura2,

Nareeluk Nakaew3 Kenichiro Ishida4, Masaki Yoshida4
1Princess Chulabhorn Science High School Phitsanulok
2Seishin Gakuen High School and Junior High School

2Naresuan University
4Tsukuba University

Abstract
This collaborative research studied structures and morphology of fungi in mold
starter using serial dilution technique to separate fungi from the starter. The morphology
of the separated fungi examined with a 400x microscope revealed Aspergillus spp.
Then, temperatures and humidity affecting the growth of fungi in mold starter were
investigated. Among the temperatures studied, i.e., 10°C, 25°C, 30°C, 37 °C and
40°C, the result showed that the most appropriate temperature for the growth of
separated fungi was 37°C and the most appropriate pH value was 3.0. After that, the
fungi grown in culture medium with pH value of 3.0 and incubated in temperature
of 37°C were taken to examine its inhibition properties against pathogenic bacteria
found in citrus plants by disc diffusion method. It was found that the mold starter can
inhibit the bacteria.
Keywords : mold starter, pathogenic bacteria

92

PCSHS Satun with Furukawa Reimei Junior and Senior High School
Year 2017 : 1.Thestudyoftrees’heightandcanopycoverinfrontofPetradormitory,
Princess Chulabhorn Science High School Satun and at Furukawa Shrine

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The study of trees’ height and canopy cover in front of Petra dormitory,
Princess Chulabhorn Science High School Satun and at Furukawa Shrine

Mr. Peerapol Manopak1, Miss Khommapat Wiriyasuk1,
Miss Piyamaporn Pongnarudech1, Miss Sasicha Sadeen1, Mr. Shotaro Mino2,

Miss Karen Ito2, Miss Aya Sasaki2, Miss Yui Sato2
Advisors : Mrs. Soonlaya Saman1, Miss Thanitha Pansawang1

1Princess Chulabhorn Science High School Satun
2Furukawa Reimei Junior and Senior High School

Abstract
GLOBE (Global Learning and Observations to Benefit the Environment) Program
is an international science and education program that provides students and the public
worldwide with the opportunity to participate in data collection and the scientific
process, and contribute meaningfully to our understanding of the Earth system and
global environment. Therefore, we want to contribute to the program by studying the
tree’s height and canopy cover in our area. The canopy cover can affect the density
and amount of light that shines through the forest floor of the forest. In our study, we
measure the canopy cover in the study area by using densiometer and measure the
tree’s height in the study area by using clinometer. From the surveys and calculations,
the tree’s height and circumference can help determine rate of plant growth in the
study area. Percent of canopy cover is 50.54 percent which is greater than 40 percent.
That means the front of Petra dormitory, Princess Chulabhorn Science High School
Satun and Furukawa Shrine near Furukawa Reimei Junior and Senior High School
have a large density of trees.
Keywords : GLOBE, canopy cover, density

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UV LED Generator

Jiratchaya Chanakul1, Natchanon Nirapai1, and Chawin Keyanon1
Tomoaki KATO2 ,Yusuke SAWADA2, Hiroki SUGIYAMA2, Keiji MINAMINO2

Advisors : Siripong Srisuwan1, Yoshio NISHIDA2, Pansak Kerdthogmee3
1Princess Chulabhorn Science High School Nakhon Si Thammarat
2Nara Prefectural Seisho High School
3Walailak University

Abstract
This Science project was to study and test the UV LED generator. The purpose
of the project was to study the UV LED with a wavelength in the range of 395-400
nm by using black light bulbs to generate UV rays. The voltage of each UV LED was
recorded. Then the relationship between the number of UV LED and the model
of circuit were compared to find out the highest average power one. Later, the
experiment of electricity generated from UV rays of sunlight was to compare. And
the examination of the most effective electricity generating of four panels of two
circuit models, the series-connected UV LED and the parallel-connected UV LED
was conducted. The results showed that UV LED could be an electricity generator
from black light bulbs. With the 50 UV LEDs parallel-connected, the average voltage
was 0.68 mW per panel. When the panels were series-connected, of 2, 3 and 4, the
average voltages were 43.68, 47.57 and 58.16 mW respectively. When the panels were
parallel-connected, of 2, 3 and 4, the average voltages were 25.85, 36.36 and 62.08
mW respectively. It can be concluded that the 4 parallel-connected panels is the most
effectiveness in generating electricity of 62.08 mW

Keywords : UV LED, Ultraviolet ray, Average Electricity Power

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The study of morphological and the UV-Visible absorption of
the Sangyod Rice

Kricha Suksangpleng1, Pordee Phetsiri1
Norimichi SHIBATA2, Yusuke SUGIMURA2,

Rei TSUJI2, Sara HASHIZUME2
Advisors : Kanokrat Singnui1, Nahoko TAKEUCHI2, Watthana Rathanaphom3

1Princess Chulabhorn Science High School Nakhon Si Thammarat
2Nara Prefectural Seisho High School
3Suratthani Rajabhat University

Abstract
The study of morphological and the UV-Visible absorption of the Sangyod
Rice varieties in 5 areas of south of Thailand, Paphayom district, Khuan Khanun
district, Khao Chaison district Bang Saphan sub-district Nakhon Si Thammarat and
Nakhon Si Thammarat Rice research center. The absorption of anthocyanin has been
performed by using UV-Visible spectroscopy technique.
The result shows the morphological of the Sangyod rice, the highest Ratio of length
to width is the Nakhon Si Thammarat Rice research center’s seeds equal to 3.73 ±
0.11 and the lowest is the Paphayom district’s seeds equal to 3.31 ± 0.21, all grain
shape were slander. The highest of 100 seeds weight is 1.75 ± 0.04 g. The rice grain
from Nakhon Si Thammarat Rice research center’s seeds has the highest percentage
cracking, 69.93 ± 2.72 %. The Bang Saphan sub-district shows the highest UV-Visible
absorption at 520 nm. is 0.65 ± 0.03 and the lowest is Khuan Khanun district rice
grains equal to 0.49 ± 0.01.

Keywords : Morphological, Sangyod Rice, Anthocyanin

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Contributors for Academic Cooperation
of Japan to Princess Chulabhorn Science High Schools

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Editor

Advisors

Dr. Kosol Petchsuwan Advisor to Steering Committee of PCSHS

Dr. Thongchai Thongchai Chewpreecha Advisor to Steering Committee of PCSHS

Dr. Yuvadee Nakapadungrat Advisor to Steering Committee of PCSHS

Ms. Potchanee Chanpanus Steering Committee of PCSHS

Dr. Phuriwat Kamaikawin Director of Excellence in Science Education

Bureau, OBEC

Mr. Poramet Charoynoot Director of Promotion of Academic

Cooperation Division,

Excellence in Science Education Bureau, OBEC

Principals

Dr. Wansawang Singchai Princess Chulabhorn Science High School

Chiangrai

Dr. Supote Thanwong Deputy Principal Princess Chulabhorn Science

High School Chiangrai

Mr. Jeerasak Janta Deputy Principal Princess Chulabhorn Science

High School Chiangrai

Miss Pudsadee Sommana Deputy Principal Princess Chulabhorn Science

High School Chiangrai

Miss Kanokporn Wongchaleekul Deputy Principal Princess Chulabhorn Science

High School Chiangrai

Mrs. Sureerat Boonchan Deputy Principal Acting for Principal

Princess Chulabhorn Science High School

Phitsanulok

Mr. Kittichai Kruaithong Princess Chulabhorn Science High School Loei

Mr. Prakart Ontam Deputy Principal Acting for Principal

Princess Chulabhorn Science High School

Mukdahan

Dr. Sming Kultee Princess Chulabhorn Science High School

Lopburi

Mr. Sak Rungsang Princess Chulabhorn Science High School

Burirum

Dr. Samorn Pato Princess Chulabhorn Science High School

Pathumthani

Dr. Thaweesak Charoentia Princess Chulabhorn Science High School

Chonburi

Ms.Nipa Udomnak Deputy Principal Acting for Principal Princess

Chulabhorn Science High School Phetchaburi

Dr. Santi Nadee Princess Chulabhorn Science High School

Nakornsri thanmmarat

Dr. Suwanee Uengwarakorn Princess Chulabhorn Science High School

Trang

Mr. Panya Khunritkaew Princess Chulabhorn Science High School

Satun

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