TECHNICAL PRESENTATION FOR A NEW 5-PART DIFFERENTIATION ...

MYTHIC 22

TECHNICAL PRESENTATION
FOR A NEW 5-PART
DIFFERENTIATION
TECHNOLOGY

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

MYTHIC 22

A new patented
technology for the 5
part differentiation in

haematology

Optical Cytometer
Hydrofocus Free
(OCHF)

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Optical Cytometer Hydrofocus Free
(OCHF)

100% full optical 5-DIFF technology.
Use a Solid State Light Source at a low
wavelength, 460 nm:

Shelf life of the light source: 10 years.
460 nm wavelength: enhances the
determination of the eosinophils without any
staining.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Optical Cytometer Hydrofocus Free
(OCHF):

New Optical Bench

The innovative optical detection system is
covered by two pending patents.

This technology is based on a unique and
innovative concept of an active sample flow
and a passive sheath.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Optical Cytometer Hydrofocus Free
(OCHF):

New Flow Cell

The sample flow is introduced in the flow
cell under pressure and the sheath is only
dedicated to maintain it.

This principle enables to introduce a large
volume of sample and to use a high dilution
ratio, in order to perform the haemoglobin
measurement with the same dilution.

Sample flow

Optical window

Light beam
Injector

Passive Sheath

Sample

Mythic 22 – Flow

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Optical Cytometer Hydrofocus Free
(OCHF):

Principle of Measurement

LIGHT INTENSITY

WBC CELL

OPTICAL DETECTION ZONE TIME

SAMPLE FLOW

Two measurements are performed for each
cell crossing the interrogation zone:

A measurement of Light Scattered in the
forward direction (FSC: Forward Side
Scatter).

A measurement of Light Losses (ALL:
Axial Light Losses).
The use of a low wavelength light source
enhances the scattering efficiency thus
revealing finer cell structures.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Optical Cytometer Hydrofocus Free
(OCHF):

A new Lysing solution, OnlyOne.

The five part diff is obtained by the optical
scattergram analysis after action of OnlyOne,
the new Orphée’s lysing solution (pending
patent).

This reagent destroys the RBC and their
stromas, composes the oxy haemoglobin
chromogen and protects the white blood cell
membrane to keep it in closed native state.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Optical Cytometer Hydrofocus Free
(OCHF):

A new Lysing solution, OnlyOne.

The combination of the lysing action and
the specific optical setup allows:

To move the neutrophils to the right part
of the scattergram and the basophils can
be identified above and between the
lymphocytes and monocytes.

To shift to the left the eosinophils to
ensure a large gap between them and the
neutrophils thus rugged boundary
detection.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Optical Cytometer Hydrofocus Free
(OCHF):

A new Lysing solution, OnlyOne.

This innovative technique avoids the use of
a specific dye or channel measurement for
the eosinophils detection and a specific
channel measurement for the basophils.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Optical Cytometer Hydrofocus Free
(OCHF):

Scattergram Presentation

ALL Neutrophils
Eosinophils
Monocytes
Basophils FSC

Lymphocytes

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22 – General Overview

Dispensing Module Optical Bench
Optical Manifold

Counting Module Syringes Block

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22
Technical Specifications

Hydraulic:

Sample volume < 20 µl.
Only 2 dilutions:

1:170 Ö WBC – HGB – 5-DIFF.
1:10.000 Ö RBC – PLT.
Only 3 reagents : Diluent, Cleaner,
OnlyOne Lytic solution;
All the hydraulic and optical parts heated at
34°C.

Display:

TFT Colour LCD 240*320, Retro-lighted.

Power:

External power supply, 24V.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22
Physical Specifications

Width : 250 mm 11 kg

Height : 350 mm

Depth : 340 mm
Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology

© Orphée S.A. – November 2005

Mythic 22
Technical Specifications

Software:

Embedded software including:
Calibration Menu.
QC Menu: 6 batches, 100 run per lot
with table and Levey-Jennings graph.
Patients archive: up to 500 patients.
Connection:

2 USB ports.
RS232 port and Ethernet port.
Centronics ports.
Barcode reader.
Bidirectional connection to SVM software
(Validation station for Mythic line.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

MYTHIC 22
RESULTS

NEU

M22100,0 y = 0,R9287=80x,+9706,58612
90,0
80,0 100,0
70,0
60,0 80,0
50,0
40,0 60,0
30,0
20,0 20,0 40,0 P120
10,0
0,0
0,0

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22
Comparative Evaluation

The presented results are versus Pentra 120™
(Horiba Abx™).

RBC and associated parameters

RBC HGB

M22 7,00 M22 M22 25,0 y = 0,9791x + 0,21
6,00 20,0 R2 = 0,9941
5,00 y = 0,9356x + 0,2301 15,0
4,00 R2 = 0,9837 10,0 5,0 10,0 15,0 20,0 25,0
3,00 P120
2,00 2,00 4,00 6,00 8,00 5,0
1,00 0,0
0,00 P120
0,0
0,00

M2260,0 HT 140,0 MCV
50,0 120,0
40,0 y = 0,8501x + 5,3347 100,0 y = 0,9631x + 3,5265
30,0 R2 = 0,9721 R2 = 0,8982
20,0 80,0
10,0 10,0 20,0 30,0 40,0 50,0 60,0 70,0 60,0 20,0 40,0 60,0 80,0 100,0 120,0 140,0
40,0 P120
0,0 P120 20,0
0,0
0,0
0,0

™: Pentra 120 and Horiba Abx are Horiba Abx trademark.

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22
Comparative Evaluation

Platelet and associated parameter

PLT VMP

1000 12,0
900
800 10,0
700
600 8,0
500
M22400 6,0
300
M22200
100
0 4,0
0
2,0 y = 0,8015x + 1,7543
R2 = 0,8085
y = 0,9083x + 18,194
R2 = 0,9811 0,0
0,0 2,0 4,0 6,0 8,0 10,0 12,0
200 400 600 800 1000 1200 P120
P120

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22
Comparative Evaluation

WBC and 5-Part differentiation

WBC

30,0

25,0

20,0

M2215,0

10,0

y = 0,9195x + 0,6222
5,0 R2 = 0,9945

0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0
0,0 P120

NEU EOS

M22 100,0 M22 M22 14,0
80,0
60,0 20,0 y =0,9878x + 0,5612 12,0 y = 0,8402x + 0,1817
40,0 R2 =0,9768 R2 = 0,9031
20,0 10,0
0,0 40,0 60,0 80,0 100,0 10,0 15,0
0,0 P120 8,0

LYM 6,0 5,0
P120
60,0 4,0
MON
2,0
50,0
0,0
40,0 0,0

M2230,0 20,0
18,0
20,0 y = 1,009x - 0,2862 16,0 y = 0,9377x + 0,4536
R2 = 0,9788 14,0 R2 = 0,876
10,0 12,0
10,0 20,0 30,0 40,0 50,0 60,0 10,0 5,0 10,0 15,0 20,0
0,0 P120 P120
0,0 8,0
6,0
4,0
2,0
0,0

0,0

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22
Some abnormal cases

1. Leucopenia

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22
Some abnormal cases

2. Leucocytosis

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Mythic 22
Some abnormal cases

3. Eosinophilia

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005

Orphée S.A
19 chemin du Champ des Filles

1228 Plan les Ouates
SWITZERLAND

Phone : +41.(0)22.706.18.40
Fax : +41.(0)22.794.43.91

e-mail : [email protected]
Distributor :

Mythic 22 – Technical Presentation for a new 5-Part Differentiation technology
© Orphée S.A. – November 2005