BT STAR '20
Module catalogue
BT STAR ‘20
BT STAR
A Story Telling on Advanced research
The Inception
A Tlmost a year back, a great he journey was not as smooth
initiative was taken by Dr.as this sounds. Providing
KPC Life Sciences Pvt. Ltd. To hands on training to 100 of stu-
disseminate basic, necessary and dents by two or three resource
relevant technologies in Molec- persons in a day or two, was not
ular Biology, we were moving actually an easy job. We were
in different parts of India. First very much focused on giving
roadblock, instruments were not hands on training to each and
available or ready to use in some every individual student partic-
of the institutes. So, We decid- ipating. Tough to believe, but
ed to bring not only the reagents, true, one of the training module
but the required instruments too, covered gene expression studies
with us. We approached sever- by real time PCR. From each and
al Universities throughout the every training program, we select-
country. This was a brand new ed few students with whom we
proposal to the institutes, a bio- are going to conduct a National
tech company is coming with all Symposium in our Kolkata cam-
the instruments and reagents to pus. We named this program BT
provide hands on training. We re- STAR (Biotech Training-Story
ceived an overwhelming response. Telling on Advanced Research).
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BT STAR ‘20
“Moonlight
drowns out
all but the
brightest
stars”
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BT STAR ‘20 From the
chairman’s
desk
“Knowledge is not attained“
by chance, it must be
sought for, with ardour
and attended to, with
diligence.
- Dr. K.P. Chaudhuri
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BT STAR ‘20
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BT STAR ‘20
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BT STAR ‘20
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BT STAR ‘20 meet
our
traiNers
Dibyajnan
Aritrika We are training specialists and
our trainers are authoritative,
Sriparna focussed and inspirational edu-
cators. Our creative, interactive
Maitrayee and relevant sessions ensure par-
ticipants are actively engaged
“Moumita from the outset, ready to apply
what they have learnt as soon
Equipping the best and brightest as they are back at their desks.
minds with the right skills and Each training programme uses
knowledge is crucial to success in a combination of instruction,
our increasingly competitive and worked examples and group dis-
complex industry. Playing a part cussion which embeds the prac-
in that is both immensely satisfy- tical application of our course
principles in their workplace.
ing and incredibly motivating.
-Dibyajnan Chakraborty,
Senior Research Scientist
7 ‘‘
BT STAR ‘20
Modules
for
BT STAR
‘20
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BT STAR ‘20 Module 1
MOLECULAR DIAGNOSTICS 1:
A closer look into your genes
Hands on training: Discussion and
theory sessions:
• eco’ Blood DNA Isolation
• gDNA quantification and • DNA isolation proto-
col principle
quality check by agarose
gel electrophoresis • ARMS PCR Principle
• ARMS PCR Set-up for • Brief overview of
Sickle cell anemia muta-
tional analysis sickle cell anemia
• Agarose Gel Electropho-
resis of PCR products
• Data Analysis
Duration: 3 days Certificate: Yes
Location: Anywhere Minimum no. of
in India students: 48*
Course fee: Rs 3000
*For number of students> 48, additional instrumentation
support will be required from participating institute.
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Module 2 BT STAR ‘20
MOLECULAR DIAGNOSTICS 2:
Analysis by RFLP
Hands on training: Discussion and
theory sessions:
• eco’ Blood DNA Isola-
tion • DNA isolation protocol
• gDNA quantification principle
and quality check by • RFLP Principle
agarose gel electropho-
resis • Brief overview of Lac-
tose intolerance
• Lactose intolerance by
RFLP analysis
• Agarose Gel Electro-
phoresis of digested
products.
• Data Analysis
Duration: 3 days Certificate: Yes
Location: Anywhere Minimum no. of
in India students: 48*
Course fee: Rs 3000
*For number of students> 48, additional instrumentation
support will be required from participating institute.
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BT STAR ‘20 Module 3
RFLP: A molecular tool
for identifying variations
Hands on training: Discussion and theory
sessions:
• eco’ Bacterial genomic
DNA Isolation • DNA isolation protocol
• gDNA quantification and principle
quality check by agarose
gel electrophoresis • PCR principle
• PCR amplification • RFLP Principle
• RFLP analysis
• Agarose Gel Electro-
phoresis of digested
products.
• Data Analysis
Duration: 2 days Certificate: Yes
Location: Anywhere Minimum no. of
in India students: 48
Course fee: Rs 2000
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Module 4 BT STAR ‘20
DNA & PROTEIN ISOLATION:
Delving into E.coli cells
Hands on training: Discussion and theory ses-
sions:
• eco’ Bacterial genomic • DNA isolation protocol
DNA Isolation principle
• gDNA quantification and
quality check by agarose gel • 16S PCR principle
• SDS PAGE principle
electrophoresis
• 16S rDNA PCR from isolat-
ed gDNA
• Agarose gel electrophoresis
of PCR product
• Protein isolation from bac-
terial culture & loading on
SDS PAGE
• Gel staining, de-staining &
Data analysis
Duration: 2 days Certificate: Yes
Location: Anywhere Minimum no. of
in India students: 48
Course fee: Rs 1750
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BT STAR ‘20 Module 5
THE EXCEPTION OF CENTRAL DOGMA:
Reverse Transcription
Hands on training: Discussion and
theory sessions:
• eco’ Bacterial RNA Isolation • RNA isolation protocol prin-
• RNA quantification and
ciple
quality check by agarose gel
electrophoresis • Reverse transcriptase princi-
ple
• cDNA synthesis from isolated
RNA • PCR principle
• PCR from prepared cDNA
• Agarose gel electrophoresis
of PCR product.
Duration: 3 days Certificate: Yes
Location: Anywhere Minimum no. of
in India students: 48*
Course fee: Rs 3000
*For number of students> 48, additional instrumentation
support will be required from participating institute.
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Module 6 BT STAR ‘20
MOLECULAR SCISSOR AND GLUE (Intermediate):
Restriction Digestion and Ligation
Hands on training: Discussion and
theory sessions:
• Restriction digestion of • Brief introduction
vector
about restriction en-
• Vector digestion check zymes
and quantification by
agarose gel electropho- • How to set up a restric-
resis tion digestion
• Calculation for setting • Ligation ratio and li-
up ligation gation set up
• Setting up of ligation
reaction
• Agarose gel electropho-
resis of ligated product
Duration: 2 days Certificate: Yes
Location: Anywhere Minimum no. of
in India students: 50
Course fee: Rs 1500
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BT STAR ‘20 Module 7
MOLECULAR SCISSOR AND GLUE
(Expert):
Restriction Digestion, PCR and Ligation
Hands on training: Discussion and theory
sessions:
• PCR amplification of insert
• Restriction digestion of insert • Brief introduction about re-
striction enzymes
and vector
• Vector and insert digestion • Brief theory of PCR
check and quantification by • How to set up a restriction di-
gestion
agarose gel electrophoresis
• Ligation ratio and ligation
• Calculation for setting up li- set up
gation
• Setting up of ligation reaction
• Agarose gel electrophoresis
of ligated product
Duration: 3 days Certificate: Yes
Location: Anywhere Minimum no. of
in India students: 50
Course fee: Rs 2000
15
Module 8 BT STAR ‘20
DATA ANALYSIS (Beginner):
The power of interpretation
Discussion and theory sessions:
• Gel Visualization and data analysis
• ARMS PCR data analysis in molecular
diagnostic
• Real time PCR data analysis
• DNA sequencing data analysis
• Sequencing data alignment
• Phylogenetic tree formation
• Bioinformatic tools: BLAST, MSA,
CLustalW, in-silico digestion, Primer
BLAST, Plasmid drawing
Duration: 1 day Certificate: Yes
Location: Anywhere Minimum no. of
in India students: 75
Course fee: Rs 1500
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BT STAR ‘20 Module 9
DATA ANALYSIS (Expert):
The Power of Interpretation
Discussion and theory Discussions and theory
sessions: sessions:
• Gel Visualization and data • Bioedit
analysis • Snapgene
• Image Quant
• PCR data analysis in mo- • Biorad CFX Manager
lecular diagnostic • EXPASY translate tool
• NEB Cutter V2
• Real time PCR data analy- • MEGA 4.0
sis
Duration: 2 days Certificate: Yes
• DNA sequencing data
analysis Location: Anywhere Minimum no. of
in India students: 75
• Sequencing data alignment Course fee: Rs 1800
• Phylogenetic tree forma-
tion
• Bioinformatic tools:
BLAST, MSA, CLustalW,
in-silico digestion, Primer
BLAST, Plasmid drawing
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BT STAR ‘20
BT STAR ‘19
A Retrospective...
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BETmiaSnnTednAtiRnsdp‘ 2ueas0tkreyr,s gfrraomcedactahde eemveiant.
Prof. Syamal Roy (Dean, Prof. Dhrubajyoti Chatto-
NIPER-Kolkata) present- padhyay (Vice Chancellor,
Amity University, Kolkata),
ing on “DNA Vaccine” during his talk, titled “Bio-ex-
cite”, which dealt with the
newest, cutting-edge technol-
ogies on frontiers of biotech-
Prof. Sujoy Kumar Dasgupta (Sci- Prof. Maitreyee Bhattacharyya
entist, Bose Institute, Kolkata) (Director, IHTM, Medical Col-
delivering his lecture on “TB Re- lege, Kolkata), delivering her
search & Drug Development” talk on “ Beta Thalassemia: De-
tection by Molecular Diagnostic”
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BT STAR ‘20
Mr. Arindam Ghatak
(Co-founder & Head-R&D, Bi-
omoneta, Bangalore) , throw-
ing light on the topic “Elec-
trodynamic removal of
Pathogenic Microorgan-
isms from healthcare en-
vironments: Preventing
Infections, saving lives”.
Dr.Sanjib Dey (Scientist III,Thermo Mr. Priyam Sengupta (Founder,
Fisher), during his talk on “From Bloodmates) creating awareness
Samples till Screening: Solutions on blood donation and premarital
as a Partner in Molecular Diagnos-
tics” thalassemia screening.
Prof. Nirmalya Roy (MD,
Medicine, KPC Medical Col-
lege & Hospital), during his
talk on “Pathogenesis of
Type 2 Diabetes Mellitus”
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BT STAR ‘20
Poster Presentation
by students
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BT STAR ‘20
Mr. Dhruba Chatterjee from BEST
Andragogy, receiving his Posters
award for best poster
Miss Samanwita Mandal of
Adamas University, receiving
her trophy and certificate for
best poster.
Miss Srabani Roy Chowdhury of 22
Techno India University, receiv-
ing her best poster award
Mr. Subhankar Chowdhury,
from Asian Institute of
Management and Technol-
ogy (Assam) being awarded
for best poster.
Mr. Pratik Chatterjee from Techno
India University, being awarded
the prize for best poster
BT STAR ‘20 SomemCoampetnutrsed
Intrigued Audience
Team KPCLS
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BT STAR ‘20
AWARD LIST
Best Organizing Institute: Asian Institute of
Management &
Technology, Assam
Maximum number of ADAMAS UNIVERSITY
participants:
Maximum Module Coverage: A N D R A G O G Y
Best Interaction: Mr. Dhruba Chatterjee
(A n d ra g o g y )
Outstanding support: Rima Roy (Adamas
U n iv e rs i ty )
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Best Poster
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Best Poster 26
Best Poster
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Best Poster 28
Best Poster
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Feedback!!!
I am really grateful to K.P.C.LIFE TwtyvhoeeeeplrtliyacfssnoyoindccmrtugoepIsavreoeelndsers.iiatezuideIrmndntgwewdwesaayraaensmsdipblnovordotest.eriahueydemd
SCIENCES Pvt. Ltd. for giving me -Jinty Paul
an opportunity to participate on AIMT (Assam)
the National Symposium BT STAR'19
The poster session was also very well BT-STAR'20 was an ex-
arranged and it encorages us a lot. quisite, informative and a
The overall symposium was excellent communicative program
and was well organised. providing a lot of knowl-
edge and interesting facts
- Sreenath Dey -Srabani Roy Chowdhury
(Techno India University) (Techno India University)
LaagetTnnicrohdetdnuearatLiee.nelsma.iItiSrinamnyeteenmidtvtpemaopoasrfilnuoomytftweshofwsrooadonrsamsoyurtstrehhaaNeeblriallreye- TsoohisuanwoneImcnletweB,iudlnrplthTtiw-raoadtcoeSantiionsrvsTsnemrilAdaeyc-tvssResoeeeedopSrahspcfyt.murauormbcoaeTmsthhAoghlhwbeaiIdrmoseairaMnaeteusathdrmksbgTxsnyaiwhphliammari(itrbottAnnopiyhiCuzopsddfole.hstrodpadseoowtuiacmuhwvbnehtmmoe)eldhdnlcyhosfoiawyenougltaxrmcstoeieyivshunggpcesiihiouvniccntet-aikategsrinydlnyd.g
(Tech-nPoraItmikdCiahaUtnteivrejreseity)
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Dr. KPC Life Sciences Pvt. Ltd. Block-5, KPC Medical College & Hospital Campus,
1F, Raja S.C. Mullick Road, Jadavpur, Kolkata, India, Pin-700032. Ph: +919830391365
+919007375343 [email protected]