PROGRAMME & ABSRACT BOOK
1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024
CONTENTS PROGRAMME ABSTRACT 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 W1 - Yatavee Teyton (KU) : The application of machine learning in aquaculture for water quality prediction W2 - Phaczarun Tungsomkid (KU) : Adoption on Good Aquaculture Practices (GAP) of Nile tilapia farmers in Samut Prakan Province W3 - Sofiyudin Maae (PSU) : Plastic ingestion by three species of Scylla (Brachyura) from the coastal areas of Thailand W4 – Rendiansyah (UNHAS) : Study of physico-chemical parameters in natural habitats seaweed (Asparagopsis taxiformis) W5 - Dedy Muhammad Iqbal (UNHAS) : Developing new tehchnique for seaweed cultivation: zkappaphcus sp culturing at ponds W6 - Anirut Klomjit (KU) : Species diversity and distribution pattern of brown seaweed genus Lobophora from the Gulf of Thailand B1 - Arfiani Rizki Paramata (UNHAS) : DNA barcoding and eDNA metabarcoding in one of the National Critical Lake Waters, Lake Limboto, Sulawesi, Wallacea B2 - Ihsan Hani binti Radzi (PSU) : Phenotypic variation of Thenus spp. (Decapoda, Scyllaridae) in the waters of southern Thailand and Malaysia using multivariate morphometric analysis B3 - Pawida Prasopsook (KU) : Conservation efforts for cephalopod resources involve the establishment of octopus banks, the monitoring of this species in their natural habitats around Chao Sam Ran Beach, Phetchaburi Province B4 – Gusti Afiz Gusti Ruslan Noor : Morphometric and genomic evaluation of local and imported penaeid shrimp from Malaysia in determining the potential local broodstock candidate. B5 - Huang Lei Heng (BGU) : Potential application of horseshoe crabs and microbenthic community structure to indicate anthropogenic impacts on mangrove wetlands in northern Beibu Gulf, China PAGE 1 4 5 6 7 8 9 10 11 12 13 14
CONTENTS 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 B6 - Amirul Ashyraf Mohd Roshini : Identification of Growth-Associated Genes By Genomic-Wide Association Study (GWAS) For The Production of Green Tiger Shrimp, Penaeus semisulcatus (De Haan, 1844) Quality Broodstock B7 - Sri Ayu Lestari (UHNAS) : Analysis bioecological study of the largetooth flounder (Pseudorhombus arsius Hamilton, 1822) in Spermonde C1 - Wang Wei (BGU) : Does food affect Tri-spine horseshoe crab distribution in nursery habitats: findings from northern Beibu Gulf, China C2 - Abdul Hafiz Ismail (UMT) : Interactive impact of temperature and salinity on physiological adaptability of Pacific whiteleg shrimp Litopenaeus vannamei C3 - Hafeezee Chema (PSU) : Producing berried female mud crab (Scylla paramamosain) without eyestalk ablation in water recirculation system C5 - Hanan Hayimadeeyoh (PSU) : Embryonic development of Khela Mahseer Tor douronensis (Valenciennes, 1842) in Southern Thailand P1 - Dong Yaxin (BGU) : Deciphering the molecular landscape: FpVgR and its significance in ovarian development of Fenneropenaeus penicillatus P2 - Sylvia Ng Sin Ee (UMT) : Characterisation of the androgenic gland, full sequence cloning of the Insulin-like androgenic gland (IAG) gene, and its spatial and temporal expressions in the orange mud crab, Scylla olivacea P3 - Saleh Ahmed (UMT) : Understanding the sex differentiation process in mud crab Scylla olivacea and the effects of methyl testosterone and estradiol P4 - Yosapon Adisornprasert (KU) : Effects of dietary supplementation of estradiol-17β on Monosexual all-female in long- whiskered catfish, Mystus gulio (Hamilton, 1822) PAGE 15 23 24 16 17 18 19 20 21 22
Date Time Activity 28 Feb 2024 0900-1000 Registration 1000-1030 Tea break 1030-1300 Ice-breaking activity at AKUATROP 1300-1400 Lunch 1400-1410 Speech from UMT Vice Chancellor 1410-1420 Speech from the Director of AKUATROP 1420-1430 Speech from the Organising Director of IPSOS 1430-1450 UMT-BGU MoU Exchange Ceremony & the Opening Ceremony of IPSOS 1450-1500 Group photo 1500-1530 Tea break 1530-1700 Keynote sessions and introduction to each respective universities (1 keynote speaker from each university, 15 min) 1530-1545 UMT (Dr. Waiho) 1545-1600 BGU (Prof. Billy Kwan) 1600-1615 UNHAS (Prof. Yushinta) 1615-1630 KU (Dr. Chontida) 1630-1645 PSU (Dr. Sofiyuddin) 1700 onwards Free activity 29 Feb 2024 0830-0930 Presentation from 6 participants (each participant is allocated 10 min) 6 participants 0830-0840 W1 - Yatavee Teyton (KU) : The application of machine learning in aquaculture for water quality prediction 0840-0850 W2 - Phaczarun Tungsomkid (KU) : Adoption on Good Aquaculture Practices (GAP) of Nile tilapia farmers in Samut Prakan Province 0850-0900 W3 - Sofiyudin Maae (PSU) : Plastic ingestion by three species of Scylla (Brachyura) from the coastal areas of Thailand 0900-0910 W4 – Rendiansyah (UNHAS) : Study of physicochemical parameters in natural habitats seaweed (Asparagopsis taxiformis) 0910-0920 W5 - Dedy Muhammad Iqbal (UNHAS) : Developing new tehchnique for seaweed cultivation: zkappaphcus sp culturing at ponds 0920-0930 W6 - Anirut Klomjit (KU) : Species diversity and distribution pattern of brown seaweed genus Lobophora from the Gulf of Thailand 0930-1030 Discussion and sharing session PROGRAMME 1
Date Time Activity 1030-1040 Tea break 1040-1200 Presentation from 7 participants (each participant is allocated 10 min) 7 participants 1040-1050 B1 - Arfiani Rizki Paramata (UNHAS) : DNA barcoding and eDNA metabarcoding in one of the National Critical Lake Waters, Lake Limboto, Sulawesi, Wallacea 1050-1100 B2 - Ihsan Hani binti Radzi (PSU) : Phenotypic variation of Thenus spp. (Decapoda, Scyllaridae) in the waters of southern Thailand and Malaysia using multivariate morphometric analysis 1100-1110 B3 - Pawida Prasopsook (KU) : Conservation efforts for cephalopod resources involve the establishment of octopus banks, the monitoring of this species in their natural habitats around Chao Sam Ran Beach, Phetchaburi Province 1110-1120 B4 – Gusti Afiz Gusti Ruslan Noor : Morphometric and genomic evaluation of local and imported penaeid shrimp from Malaysia in determining the potential local broodstock candidate. 1120-1130 B5 - Huang Lei Heng (BGU) : Potential application of horseshoe crabs and microbenthic community structure to indicate anthropogenic impacts on mangrove wetlands in northern Beibu Gulf, China 1130-1140 B6 - Amirul Ashyraf Mohd Roshini : Identification of Growth-Associated Genes By Genomic-Wide Association Study (GWAS) For The Production Of Green Tiger Shrimp, Penaeus semisulcatus (De Haan, 1844) Quality Broodstock 1140-1150 B7 - Sri Ayu Lestari (UHNAS) : Analysis bioecological study of the largetooth flounder (Pseudorhombus arsius Hamilton, 1822) in Spermonde 1150-1300 Discussion and sharing session 1300-1400 Lunch 1400-1500 Presentation from 6 participants (each participant is allocated 10 min) 5 participants 1400-1410 C1 - Wang Wei (BGU) : Does food affect Tri-spine horseshoe crab distribution in nursery habitats: findings from northern Beibu Gulf, China PROGRAMME 2
Date Time Activity 1410-1420 C2 - Abdul Hafiz Ismail (UMT) : Interactive impact of temperature and salinity on physiological adaptability of Pacific whiteleg shrimp Litopenaeus vannamei 1420-1430 C3 - Hafeezee Chema (PSU) : Producing berried female mud crab (Scylla paramamosain) without eyestalk ablation in water recirculation system 1430-1440 C5 - Hanan Hayimadeeyoh (PSU) : Embryonic development of Khela Mahseer Tor douronensis (Valenciennes, 1842) in Southern Thailand 1440-1550 Discussion and sharing session 1550-1600 Tea break 1600-1650 Presentation from 5 participants (each participant is allocated 10 min) 4 participants 1600-1610 P1 - Dong Yaxin (BGU) : Deciphering the molecular landscape: FpVgR and its significance in ovarian development of Fenneropenaeus penicillatus 1610-1620 P2 - Sylvia Ng Sin Ee (UMT) : Characterisation of the androgenic gland, full sequence cloning of the Insulin-like androgenic gland (IAG) gene, and its spatial and temporal expressions in the orange mud crab, Scylla olivacea 1620-1630 P3 - Saleh Ahmed (UMT) : Understanding the sex differentiation process in mud crab Scylla olivacea and the effects of methyl testosterone and estradiol 1630-1640 P4 - Yosapon Adisornprasert (KU) : Effects of dietary supplementation of estradiol-17β on Monosexual all-female in long- whiskered catfish, Mystus gulio (Hamilton, 1822) 1650-1730 Discussion and sharing session 1730 onwards Free activity 1 Mac 2024 0900-1000 Roundtable reflection on IPSOS 1000-1020 Tea break 1020-1040 Award ceremony 1040-1100 Closing ceremony 1100-1110 Group photo 1110-1300 Lunch END PROGRAMME 3
ABSTRACT 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024
The application of machine learning in aquaculture for water quality prediction Author: Yatavee Teyton, Kasetsart University Advisor:Asst. Prof. Roongparit Jongjaraunsuk,Assoc. Prof. Wara Taparhudee, Kasetsart University Abstract: In the present, machine learning has a wide range of applications in both personal and industrial domains, including aquaculture. The aim of this study is to study the application of machine learning in aquaculture, specifically focusing on its application for water quality. Machine learning would entail the prediction of critical water quality parameters essential for aquatic animal husbandry, which are challenging and/or costly to measure directly, including dissolved oxygen (DO), total ammonia nitrogen (TAN), nitrite-nitrogen (NO2-N), and chlorophyll-a (Chl-a); as well as readily leverage measurable and cost-effective water quality variables such as water temperature, pH, transparency, and alkalinity by predicting the mean values of the targeted parameters through its extensive algorithms. This study expects to develop a machine learning model capable of accurately predicting water quality parameters with the predictive efficacy comparable to that of conventional measurement techniques. Furthermore, it should demonstrate the capability in forecasting prospective events relevant to aquaculture, such as the average concentration of Chl-a, and the occurrence of harmful algal blooms (redtide) which has happened in sea cage facilities within the Sriracha Reading Area in Chonburi Province. Keywords: Machine Learning, Water Quality, Water quality prediction. 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 4
Adoption on Good Aquaculture Practices (GAP) of Nile tilapia farmers in Samut Prakan Province Author: Phaczarun Tungsomkid, Kasetsart University Advisor: Asst. Prof. Sansanee Wangvoralak, Kasetsart University Abstract : Nile tilapia is a freshwater fish that is easy to raise, and has a high growth rate, delicious taste and affordable price. These factors have contributed to its widespread cultivation throughout Thailand. In 2004, the Ministry of Agriculture and Cooperatives initiated a policy to promote high-quality and standardized agricultural production, with the aim of building consumer confidence through voluntary applications for safe product certification by farmers. Also, the Department of Fisheries has been continuously implementing this policy, aligning it with the 20-year National Strategy and the Sustainable Development Goals; in order to develop safe products for both consumers and producers through Good Aquaculture Practices (GAP) with environmental considerations. However, this policy has not been as successfully as expected. To date, there are 7,540 Nile tilapia farms that have applied for GAP certification, and out of 352, 655 Nile tilapia farms, only 2,745 farms (0.84%) have certified for safe products (data as of February 16, 2024). This study therefore aims to investigate the level of adoption and the factors influencing the adoption of GAP in Samut Prakan Province, the largest tilapia farming area and production in the central region of Thailand. Data will be collected using an interview with structured questionnaires. Information and knowledge from this study will be useful for relevant government agencies in determining guidelines for setting and promoting policies, and encouraging more farmers to apply for safe product certification. Keywords : Nile tilapia farming; Good Aquaculture Practices (GAP); Food safety standard, Sustainable development. 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 5
Plastic Ingestion by Three Species of Scylla (Brachyura) from the Coastal Areas of Thailand Author: Sofiyudin Maae, Prince of Songkla University Advisor: Assoc.Prof.Dr. Sukree Hajisamae, Dr. Pornpimon Chuaduangpui, Prince of Songkla University Abstract: This study marked the first investigation into the presence of plastic particles in the stomachs of three mud crab species (Scylla olivacea, S. paramamosain and S. tranquebarica) collected across the Andaman Sea and the Gulf of Thailand. The highest number of plastic particles in the stomach of crab samples was polyethylene (PE) that contributed 88.5 %; while green was the predominant colour (60.3 %). Ingested particles recovered from the stomachs of crabs differed significantly between species and sites (p < 0.001). The average number of plastic particles per individual was 2.3 ± 8.6 in Scylla olivacea, 7.2 ± 16.9 in S. paramamosain, and 13.5 ± 48.9 in S. tranquebarica. Satun, revealed the highest number of plastic particles recovered from mud crabs, while the lowest number of plastic particles were from Pattani. To conclude, species of crab and site of collection plays a crucial factor in the propensity of plastic particles ingested by the genus Scylla mud crabs. Keywords: Andaman Sea, Anthropogenic marine debris, Gulf of Thailand, Mud crab 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 6
Study of physico-chemical parameters in natural habitats seaweed (Asparagopsis taxiformis) Author: Rendiansyah, Hasanuddin University Advisor: Prof. Dr. Ir. Joeharnani Tresnati, DEA, Dr. Ir. Gunarto Latama, M.Sc. Hasanuddin University Abstract: Indonesia is one of the countries that has abundant natural resources so that these resources must be managed properly in order to be sustainable. Asparagopsis taxiformis seaweed is one of the potential seaweeds to be developed because it has many benefits in terms of land animal health, Asparagopsis taxiformis seaweed is seasonal, where in certain months this seaweed is present and there are times this seaweed disappears in its natural habitat. This study aims to assess physico-chemical parameters in the natural habitat of Asparagopsis taxiformis seaweed. Both parameters will be analyzed in the natural habitat and in the laboratory of FIKP Hasanuddin University. Physical parameters analyzed were current speed, water brightness, light intensity, turbidity, temperature, and salinity. While the chemical parameters observed are pH, dissolved oxygen, ammonia, nitrate, and phosphate. Physical and chemical parameters are closely related to seaweed growth therefore it is necessary to conduct research on both parameters in the natural habitat of Asparagopsis taxiformis seaweed. Both parameters need to be analyzed as an initial rarity in terms of cultivation of Asparagopsis taxiformis seaweed. Keywords: Asparagopsis taxiformis, Physico-chemical parameters, Natural habitat 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 7
Developing new tehchnique for seaweed cultivation: Kappaphcus sp culturing at ponds Author: Dedy Muhammad Iqbal, Hasanuddin University Advisor: Prof,Dr,Ir,Ambo Tuwo, DEA, Hasanuddin University Abstract: The development of seaweed both in the world and in Indonesia is increasing but also facing various problems, therefore this research aims to overcome two main problems in cultivating Kappaphycus sp in the sea, namely the limited planting period and limited seedlings, by applying new cultivation techniques in ponds. The research method involves analyzing the availability of pond land, water quality, and environmental suitability for the growth of Kappaphycus sp. Apart from that, experiments were also carried out to study the effect of new cultivation techniques on the growth and production of seaweed. The results of the research showed that new cultivation techniques in ponds were able to overcome the limitations of planting and seedling periods that are often experienced at sea. By utilizing available pond land, farmers can significantly increase the production of Kappaphycus sp. Regulation of water quality and lighting in ponds are important factors that influence seaweed growth. Apart from that, the use of new cultivation techniques can also increase the efficiency of seed use and reduce the risk of losses due to environmental factors. This research makes an important contribution to the development of more sustainable and efficient Kappaphycus sp cultivation techniques. By implementing new cultivation techniques in ponds, it is hoped that it can increase seaweed production and provide a better alternative for seaweed farmers who face problems at sea. Apart from that, this research also opens up opportunities for further development regarding seaweed cultivation in various types of ponds with diverse environmental characteristics. Keywords: economic value, ecological value, environmental factors, biological factors, diversity 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 8
Species diversity and distribution pattern of brown seaweed genus Lobophora from the Gulf of Thailand Author: Anirut Klomjit, Kasetsart University Advisor: Assoc. Prof. Narongrit Muangmai, Asst. Prof. Jantana Praiboon, Kasetsart University, Dr. Stefano Giovanni Angelo Draisma, Prince of Songkla University Abstract: Marine brown macroalgal genus Lobophora plays important ecological roles in many marine ecosystems. This group received much attention over the past decade, and a considerable number of new species were identified globally. However, our knowledge of the genus diversity and ecology along coasts of Thailand are still limited. This study investigated the diversity of Lobophora from Gulf of Thailand using morphological data together with DNA sequences from mitochondria (cox3) and plastid (psbA and rbcL) markers. A total of 95 Lobophora specimens was collected from 30 sites in both eastern and western Gulf of Thailand. Our combined morphological and genetic data revealed the occurrence of 12 Lobophora species (L. abscondita, L. asiatica, L. boussoleae, L. challengeriae, L. chumphonensis, L. lamourouxii, L. obscura, L. pachyventera, L. quantriensis, L. thailandensis, Lobophora sp.TH1, and Lobophora sp.TH2). Lobophora chumphonensis, and L. obscura occurred both on western and eastern coasts of the Gulf, whereas L. asiatica, L. challengeriae, L.pachyventera and Lobphora sp.TH1 were confined to the eastern side, and L. abscondita, L. boussoleae, L. lamourouxii, L. quantriensis, L. thailandensis and Lobophora sp.TH2 were restricted to the western side. Our finding highlights the surprisingly high diversity of Lobophora in the Gulf of Thailand, and provides useful knowledge to support ongoing and future research on coral– macroalgal ecological relationships. Keywords: Distribution; Macroalgae; Phaeophyceae; Phylogeny; Taxonomy 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 9
DNA barcoding and eDNA metabarcoding in one of the National Critical Lake Waters, Lake Limboto, Sulawesi, Wallacea Author: Arfiani Rizki Paramata, Hasanuddin University Advisor: Prof. Joeharnani Tresnati, Dr. Nadiarti N. Kadir, Dr. Abigail Mary Moore, Hasanuddin University Abstract: Limboto Lake is one of the natural resource assets currently owned by Gorontalo Province. The problem that needs attention in the waters of Limboto Lake is the decline in the population of economically important native fish, in this case especially the Gobiiformes such as mangobai fish (Glossogobius sp.), Hulu'u, and payangka (Giuris sp.). Gobiiformes or the group of gobies is a fairly large species in the Indo-Pacific region and the Indo-Malay islands. The large number of genera and the number of species of Gobiiformes are distributed in waters, so that in identifying species from previous research or existing scientific information, it can occur that there are similar names for two different species or there are different names for one species that has a species level that is difficult to identify morphologically and strongly influenced by the objectivity of previous research. DNA barcoding and eDNA Metabarcoding to evaluate management strategies for Lake Limboto in an integrated manner looking at the potential of native fish and the diversity of lake water species that support sustainable management. This research aims to identify Gobiiformes in Lake Limboto through DNA barcoding, as well as assessing fauna biodiversity through eDNA Metabarcoding. The laboratory analysis carried out consists of several stages, namely 1) DNA extraction 2) DNA amplification 3) DNA electrophoresis 4) DNA sequencing as well as eDNA. Keywords: eDNA metabarcoding; DNA Barcoding; Gobiiformes; Limboto Lake; 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 10
Phenotypic variation of Thenus spp. (Decapoda, Scyllaridae) in the waters of southern Thailand and Malaysia using multivariate morphometric analysis Author: Ihsan Hani Binti Radzi, Prince of Songkla University and Universiti Malaysia Sabah Advisors: Assoc. Prof. Dr. Sukree Hajisamae and Asst. Prof. Dr. Pochanart Kanjan, Prince of Songkla University, Dr. Chen Cheng Ann and Dr. Audrey Daning Tuzan, Universiti Malaysia Sabah Abstract: Thenus spp. are commercially significant slipper lobsters with good aquaculture potential. This study collected population information on Thenus spp. from selected sites in southern Thailand and Malaysia to inform sustainable fisheries management of the resource. Twenty-five size-adjusted morphometric measurements were analysed using canonical discriminant function and dendrogram cluster analyses to examine patterns of phenotypic variation between sites for T. orientalis and T. indicus, respectively. Significant phenotypic variation with distinct centroids and minimal overlapping cases were observed among four sites for T. orientalis (p<0.01) and supported by cluster analysis, groupings occurring as i) Kota Kinabalu and Kudat, ii) Pattani, and iii) Nakhon Si Thammarat, best discriminated by the width of the third pereiopod’s merus, the sixth abdomen segment, and the carapace’s posterior margin. Significant phenotypic variation was also detected between six sites for T. indicus (p<0.001), with close centroids and overlapping cases forming three groups: i) Ranong and Nakhon Si Thammarat, ii) Kota Kinabalu, Tanjung Sedili and Kuala Terengganu, and iii) Pattani, best described by the widths of the second antenna and the first pereiopod’s merus, and the length of the sixth abdomen segment. Patterns of phenotypic variation observed may be attributable to environmental conditions, local adaptations, and nomadic behaviour. The findings can serve as baseline information for spatial planning in fisheries management and inform regional efforts in the sustainable exploitation of Thenus spp. Keywords: Flathead lobster, fisheries population, discriminant function, cluster analysis. 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 11
Conservation efforts for cephalopod resources involve the establishment of octopus banks, the monitoring of this species in their natural habitats around Chao Sam Ran Beach, Phetchaburi Province Author: Pawida Prasopsook, Kasetsart University Advisor: Assoc. Prof. Charuay Sukhsangchan, Dr. Yaowaluck Monthum, Kasetsart University Abstract: Conservation efforts for cephalopod resources involve the establishment of octopus banks, the monitoring of this species in their natural habitats around Chao Sam Ran Beach, Phetchaburi Province were collected from total 9 boats of octopus trap artisanal fishery around Chao Sam Ran Beach over a one-year period. The participation from descendent and new generation of fisheries community in Octopus Bank conservation as the result of octopus decreasing in the natural, lack of data biological, and quality management for octopus trap fisheries. The octopus bank for conservation and sustainable resources after releases the octopus larvae to the natural after hatching in 36 hours in planktonic stage for increases octopus resource. However, the monitoring of aquatic resources involves the establishment of an Aquatic bank model still lacking accurate results. The aims of research are monitoring to confirm the octopus larvae releasing come back to the fishing area Chao Sam Ran Beach. Keywords: Biological markers, Genetic markers, Monitoring, Octopus Bank, Chao Sam Ran Beach 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 12
Morphometric and genomic evaluation of local and imported penaeid shrimp from Malaysia in determining the potential local broodstock candidate. Author: Gusti Afiz Gusti Ruslan Noor Advisor: Dr Mohd Fazhan Hanapiah, Prof Mhd Ikhwanuddin. Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu Abstract : Penaeid shrimps, a family of twelve diverse species classified under the Order Decapoda, are highly prized in both fisheries and aquaculture. In Southeast Asia region, P. monodon is one of the popular shrimp species after Litopenaeus Vannamei due to its delicacy and high economic value. However, the P. monodon broodstock comes from different sources, such as from the wild or captivity, imported from several countries such as Thailand , Madagascar , Hawaii and Australia in order to initiate the P. monodon culture. The use of nonnative shrimp broodstock may lead to the accidental release of the larvae to the wild might cause alien strain contamination and also natural hybrid occurrence in the wild. Due to this, the comprehensive screening in the wild is needed to characterize and identify different strain of shrimp in Malaysia waters. The use of molecular markers has allowed the comprehensive classification of penaeid shrimp, which provides an accurate classification. The findings of this study will be immensely beneficial in understanding the phylogenetic relationships between local strain, alien strain and hybrid contributing to a better understanding of systematic evolution. Moreover, these findings will serve as a baseline for local shrimp broodstock selection, playing a crucial role in determining gene flow and diversity of both local and imported shrimps in the wild and shed light on potential local broodstock candidates in Malaysia. Keywords: Aquaculture; Shrimp Phylogeny; Penaeid; Shrimp Broodstock. 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 13
Potential application of horseshoe crabs and microbenthic community structure to indicate anthropogenic impacts on mangrove wetlands in northern Beibu Gulf, China Author: Huang Lei Heng, Wang Wei, Beibu Gulf University Advisor: Prof. Kwan Kit Yue, Beibu Gulf University Abstract: Coastal wetlands face increasing impacts from anthropogenic activities like overfishing, aquaculture, wastewater and industrial discharges. Horseshoe crabs are prominent macroinvertebrates in northern Beibu Gulf, China. This study explores using them and other macroinvertebrates as ecological indicators of human influence. The research focuses on the population and community structure of horseshoe crabs and other macroinvertebrates in four mangrove wetlands: Daguansha (wetland park), Ronggenshan (protected area), Zhulin (aquaculture zone), and Poweidi (industrial zone). Findings revealed: (1) There were 69 macrobenthic species with an average density and biomass of 279 individual per square-meter and 174 g per square-meter per site, respectively; (2) Trispine horseshoe crab, Tachypleus tridentatus juveniles were found in all four sites, while mangrove horseshoe crab, Carcinoscorpius rotundicauda juveniles were only found in Daguansha. The average horseshoe crab densities at Ronggenshan and Daguansha sites were both 1.22 individual per 100 square-meter, followed by Poweidi site 0.57 individual per 100 square-meter, and Zhulin site 0.02 individual per 100 square-meter; (3) In terms of age structure, Daguansha showed rapid juvenile growth, while Ronggenshan and Poweidi exhibited negative growth. Zhulin had insufficient data for age structure analysis. (4) Significant differences in Berger-Parker dominance index groups existed among sites, whereas Shannon-Wiener diversity index (H'), Pielou index (J), and Margalef index (d) differences were statistically insignificant; (5) Poweidi displayed the most intense human impact according to ABC curve analysis. These results demonstrate that various human activities affect macrobenthic community structures along outer mangrove fringes, which is useful for future mangrove habitat protection and restoration. Keywords: Macrobenthos; Horseshoe crabs; Community structure; Mangrove; Intertidal zone. 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 14
Identification of Growth-Associated Genes By Genomic-Wide Association Study (GWAS) For The Production Of Green Tiger Shrimp, Penaeus semisulcatus (De Haan, 1844) Quality Broodstock Author: Amirul Ashyraf Mohd Roshini, Universiti Malaysia Terengganu Advisor: Dr. Mohd Fazhan Mohd Hanafiah, Prof. Dr. Muhammad Ikhwanuddin Abdullah, Universiti Malaysia Terengganu Abstract: Aquaculture industries has become a major source of seafood protein and improved global seafood production. Two shrimp species, Litopenaeus vannamei and Penaeus monodon, are commonly used in the aquaculture industry due to their high economic value in domestic and foreign countries. However, Penaeus semisulcatus, also known as the Green Tiger Prawn, also has potential to become one of the alternative species candidates use in aquaculture industries all over the world due to the present of this species in the wild throughout the year. However, lacking information on the life cycles, culture conditions, fecundity, mortality rates, and broodstock and larvae quality, makes this research very important. From the previous report, P. semisulcatus live in 30 ppt salinity with sea depth around 2-300 meter. This species is seen as having the potential to be cultured due to its similarities with L. vannamei and P. monodon. This study will be focused on the selection P. semisulcatus broodstock from different population in Malaysia using population genetic approach. Also, this study will evaluate the quality of broodstock focusing on fecundity, hatching rate and salinity tolerance in culture condition and lastly to identify the Growth-associated genes using Genome-Wide Association Study (GWAS). The findings from this study can serve as a reference for further studies and aquaculture of Penaeus semisulcatus. Keywords: Penaeus semisulcatus; Salinity tolerance; Population genetic approach; Genome-Wide Association Study (GWAS). 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 15
Bioecological analysis of Largetooth Flounder (Pseudorhombus Arsius Hamilton, 1822) in Spermonde Waters Author: Sri Ayu Lestari, Hasanuddin University Advisor: Prof. Dr. Ir. Joeharnani Tresnati, DEA., Hasanuddin University Abstract: Flatfish (Pseudorhombus arsius) is a demersal fish that lives on the bottom of the water, characterized by a symmetrical non-bilateral body shape and belongs to the flatfish group. Bioecology is a field of science that studies various things about the condition of fish, both from the environment and the organism itself, such as ratio and sex, population dynamics, histology and physiology, reproductive biology, and some aspects of the environment, such as biological, physical, and chemical water quality.The findings from this experiment can play an important role in increasing the production value and utilization of next fish in Spermonde Island. In addition, the results of this study are very important to study the management mechanism so that Pseudorhombus arsius can be cultivated and not paradoxical so that it can be managed sustainably. Keywords : Flatfish, Pseudorhombus arsius, Biocology, Population, Reproduction 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 16
Does food affect Tri-spine horseshoe crab distribution in nursery habitats: findings from northern Beibu Gulf, China Author: Wang Wei, Li Ze Xin, Wei Hai Yun, Beibu Gulf University Advisor: Prof. Kwan Kit Yue, Beibu Gulf University Abstract: In this study, the population/community structures of juvenile horseshoe crabs, Tachypleus tridentatus and their potential food sources, macrobenthic invertebrates were investigated in six study sites along the coast of northern Beibu Gulf, Guangxi, China. Among the potential food sources of tri-spine horseshoe crabs, they have selectivity towards microbenthic animals, and research has shown that the distribution of horseshoe crabs is related to phytophagous individuals. Juvenile horseshoe crab primarily depended on sedimentary organic matter and seagrass resource pools. The age stage of juvenile horseshoe crabs, there is no significant difference in their distribution compared to the species of macrobenthic animals. In conclusion, the distribution pattern of juvenile T. tridentatus seemed to be affected by the food sources available on the sandy mudflats. Keywords: Tachypleus tridentatus; Macroinvertebrate; Food; Population structure 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 17
Interactive impact of temperature and salinity on physiological adaptability of Pacific whiteleg shrimp Litopenaeus vannamei ABDUL HAFIZ ISMAIL1 , MAZLAN ABD. GHAFFAR1,2,3, LIEW HON JUNG1* 1 Higher Institution Centre of Excellence (HICOE), Institue of Tropical Aquaculture and Fisheries, 2 Institute of Marine Biotechnology, 3Faculty of Environment and Marine Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. *Corresponding author: [email protected] Abstract: Pacific Whiteleg shrimp farming contributed the most economical important in shrimp farming. However, with the changing environment conditions nowadays putting shrimp farming in a great challenge. It is important to understand how the shrimp remodel their physiological needs to cope with unpredictable environment changing. This study was designed to examine the interactive effect of temperature (28 vs. 34 ⁰C) and salinity (30 vs. 20 ppt) on the physiological responses using osmorespiration assay. All juvenile shrimps were subjected to four interactive conditions as T28°CN-control (28°C + 30ppt), T28°CL (28°C + 20ppt), T34°CN (34°C + 30ppt), and T34°CL (34°C + 20ppt) for 30 days period. Results revealed that both temperature and salinity significantly affected the growth, metabolic oxygen consumption, ammonia excretion rates, and energy store for the shrimp. The condition of the high temperature was found to impose a metabolic strain on the shrimp, resulting in high food consumption and elevated osmorespiration responses relative to control, but no significant different in weight gain of the shrimp. Higher osmorespiration responses was noticed in the shrimp exposed to interactive high temperature and low salinity conditions (T34°CL), followed by high temperature and normal salinity condition (T34°CN) and normal temperature with low salinity (T28°CL) as compared to normal temperature and salinity condition (T28°CN-control). This study indicated that changing environment conditions significantly affect the cost of living which may negatively impact the growth performance as consequence. Keywords: Litopenaeus vannamei, temperature, salinity, respiration 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 18
Producing Berried Female Mud crab (Scylla paramamosain) Without Eyestalk Ablation in Water Recirculation System Author: Hafeezee Chema, Prince of Songkla University Advisor: Dr. Supat Khongpuang, Assoc. Prof. Dr. Sukree Hajisamae, Prince of Songkla University Abstract: Mud crab genus Scylla is known as mangrove crab with high potential for aquaculture. One of the main problems in a mud crab culture is lacking of broodstock and low reproductive performance. In the present study, we investigated technique to produce berried female broodstock in the small-scale broodstock production with water recirculation system without applying eyestalk ablation. The study was conducted from June 2021 to December 2023 and all relevant data was recorded. A total female broodstock 412 crabs were used, data including body weight (BW; g), carapace width (CW; cm), carapace length (CL; cm), number of spawned females, percentage of spawned female from gravid to berried female, latency period (day) and some water parameters were recorded. It was found that, the BW, CW and CL of the initial gravid female crab were 355.58±82.99 grams/ind., 11.81±0.94 cm and 8.24±0.65 cm, respectively. The number of spawned female was 270 crabs (65.53% spawned) and the latency period 27.91±21.76 day. Average water salinity, temperature, DO, pH, alkalinity, and ammonia were 34.20±0.62 ppt, 27.6±0.17 ºC, 5.09±0.29 mg/l, 8.07±0.47, 170±15.74 mg/l, and 0.08±0.11 mg/l, respectively. In summary, a small-scale production of berried female mud crab using a water recirculation system is able to regularly produce broodstock providing to mud crab hatchery in Thailand. Keywords: Mud crab culture, Berried female crab, Water recirculation system 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 19
Embryonic Development of Khela Mahseer Tor douronensis (Valenciennes, 1842) in Southern Thailand Author: Hanan Hayimadeeyoh, Prince of Songkla University Advisor: Dr. Nirattisai Petchsupa, Assist. Prof. Dr. Sarawut Chesoh, Prince of Songkla University Abstract: Tor douronensis is an economically important freshwater fish that can be found only in the Southern part of Thailand. This study examined on the artificial breeding and embryonic development of T. Douronensis inhabiting in the Hala-Bala waterfall, Hala-Bala Park, Yala province. The broodstock males and females were selected and injected with buserelin acetate (30 μg/kg body weight) and domperidone (10 mg/kg body weight) solutions. Then, they were kept in the water at a proportion of 2 females: 1 male. Their eggs fertilization started after 1 hours and 26 minutes. Firstly, their fertilized eggs were spherical in shape and demersal distribution (non-adhesive). Secondly, a blastodisc stage turned into a 2-equal-sized blastomere then divided into 4,8,16,32 and 64 cells after 10 hr and 45 min. Later, morula stage changed into blastula stage after 18 hr and 20 min, then transformed into gastrula stage after 20 hr and 37 min prior to somite stage after 40hr and 6 min. Next, optical vesicles and auditory vesicles developed after 48 hr and 37 min, and 72 hr and 45 min, respectively. After passing through fertilization phase (84 hr and 50 min), their eggs started to hatching. Recently, population of T. Douronensis is declining rapidly in Southern Thailand due to environmental and anthropogenic reasons. Therefore, it is very important to conserve this populations across artificial breeding, that could be conserve and restore their population especially in Southern Thailand. Keywords: Artificial breeding, Brood stock, Fish conservation, Hatching, Laboratory experiment 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 20
Deciphering the molecular landscape: FpVgR and its significance in ovarian development of Fenneropenaeus penicillatus Author: Dong Yaxin, Beibu Gulf University Advisor: Prof. Kwan Kit Yue, Dr. Tan Kian Ann, Beibu Gulf University Abstract: Aquatic animal breeding relies on reproductive biology, which serves as an essential foundation for artificial breeding and gonadal development. Fenneropenaeus penicillatus is a significant breeding species in southern China. The vitellogenin receptor (VgR) plays a specific role in recognizing and taking in vitellogenin during receptormediated endocytosis in the oocyte. This study found that FpVgR was specifically expressed in F. penicillatus ovary and hepatopancreas. FpVgR was localized predominantly in the nucleus in the ovary tissue and in the cytoplasm in the hepatopancreas. Silencing FpVgR led to apoptosis in both hepatopancreas and ovary tissues. FpVgR depletion upregulated the expression of ovarian peritrophin 1, ovarian peritrophin 2, and serine proteinase inhibitor 6, and downregulated that of vitellogenin, delta-9 desaturase and elongation factor 1-alpha. KEGG pathway analysis involved that Focal adhesion, Endocytosis, PI3K-Akt signaling pathway, RNA transport and ECM-receptor interaction were enriched. The findings of this experiment can play a crucial role in enhancing the success rate of shrimp artificial breeding and seedling in the future. Furthermore, the results were very important for the study of spawn maturation mechanism of F. penicillatus and provided a theoretical basis for the study of the molecular regulation mechanism during crustacean oogenesis. Keywords: Fenneropenaeus penicillatus; Vitellogenesis; FpVgR; Ovarian development. 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 21
Characterisation of the androgenic gland, full sequence cloning of the Insulin-like androgenic gland (IAG) gene, and its spatial and temporal expressions in the orange mud crab, Scylla olivacea Author: Sylvia Ng Sin Ee, Universiti Malaysia Terengganu Advisor: Ts. Dr. Khor Wai Ho, Universiti Malaysia Terengganu Abstract: Orange mud crab, Scylla olivacea stands out as a crucial species in Malaysian aquaculture given to its high commercial value. The pursuit of mono-sex culture in S. olivacea has become imperative due to greater preference for larger-sized males and less aggressive females. This study delves into characterising the androgenic gland (AG) of S. olivacea, a pivotal organ responsible for secreting the insulin-like androgenic gland (IAG) hormone that plays a regulatory role in crustaceans’ sex differentiation. We will identify the location, and characterise the microscopic composition of AG cells in S. olivacea by using histological methods. Subsequently, the complete sequence of the IAG gene will be cloned using the rapid amplification of cDNA ends (RACE) approach. Then, the spatial and temporal expression patterns of the IAG gene across various tissues of S. olivacea are elucidated through quantitative real-time PCR (qPCR). This comprehensive study aims to provide insights into the AG and IAG genes, with potential applications in advancing biomolecular-based mono-sex culturing of S. olivacea. This study is anticipated to contribute as a baseline reference, addressing previously unanswered questions and fostering future developments in mud crab aquaculture. Keywords: androgenic gland, insulin-like androgenic gland hormone, Scylla olivacea, molecular cloning 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 22
Understanding the sex differentiation process in mud crab Scylla olivacea and the effects of methyl testosterone and estradiol Author: Saleh Ahmed, University Malaysia Terengganu Advisor: Prof. Muhammad Ikhwanuddin Bin Abdullah, Khor Wai Ho, Mohd Fazhan Bin Mohd Hananiah, University Malaysia Terengganu, Ma Hongyu,Shantou University Abstract: Scylla olivacea is popularly used for aquaculture because of its availability and hardy nature. But the crab aquaculture industries depend on the availability of wild-caught juveniles, which is threatened by climate change, overexploitation, cannibalism and other man-made hazards. To ensure resource sustainability, mud crab seed production is required to assist commercial farming. In animals, sexual differences emerge during embryogenesis, but the genetic composition of males and females differs slightly. The sex differentiation process varies with species. The effect of administration of steroid hormones to produce monosex culture, improve growth rate and survivability of S. olivacea remains unclear. The main focus of this study is to understand the mechanisms behind the sex differentiation process and the effects of methyl testosterone and estradiol during the early life cycle of the orange mud crab. The sex differentiation process will be performed at the physiological, morphological, and molecular levels. This will help identify the possible environmental and endogenous factors that are involved in the early stages of sex differentiation. Methyltestosterone and estradiol will be used via feeding trials. Monosex mud crab seed production technology will be uncovered through this study. This monosex crab production will provide a source of crab seeds for the enhancement and expansion of the aquaculture industry. This study will provide a more cost-effective, time-efficient, and sustainable source of crab seeds to understand the impact of anthropogenic factors and climate change on sex differentiation of crustaceans, as well as improve the quality of the end products. Keywords: Scylla olivacea; Sex differentiation; Methyltestosterone; Estradiol. 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 23
Effects of dietary supplementation of estradiol-17β on Monosexual all-female in longwhiskered catfish, Mystus gulio (Hamilton, 1822) Author: Yosapon Adisornprasert, Kasetsart University Adivisor: Dr. Phunsin Kantha, Dr. Sahabhop Dokkaew, Kasetsart University Abstract: long-whiskered catfish (Mystus gulio) belongs to the Bagridae family. This brackish water fish, commonly found at the estuary of the Bang Pakong River in Thailand. long- whiskered catfish is also a species that can tolerate changes in salinity well, able to thrive in salinities ranging from 0-30ppt. It's recognized as a promising aquaculture species due to its palatable flesh, ease of culture, and culinary versatility. Female long-whiskered catfish exhibit larger sizes than males, rendering them more economically valuable to fish farmers. This research endeavors to employ 17β-estradiol hormone, a widely-used agent in aquaculture sex reversal, to achieve a monoculture of allfemale long-whiskered catfish. The experiment entails incorporating synthesized hormones into fish feed at varying concentrations 0, 30, 60, and 90 μg The trial is replicated thrice, with 30 individuals allocated per 50-liter glass tank. Feeding commences post yolk sac absorption, administering powdered feed twice daily, at one gram per feeding, morning and evening, spanning 30 days. Sampling is conducted every 5 days throughout the 30 day period, with specimens preserved in 10% formalin for subsequent histological assessment to monitor gonadal changes. Keywords: Mystus gulio; long-whiskered catfish; 17β-estradiol; sex reversal. 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS) Advancing Green Sustainability on Marine Conservation and Aquaculture Universiti Malaysia Terengganu, Terengganu, 28 FEBRUARY - 1 MARCH 2024 24
The 1 st International Postgraduate Symposium on Ocean Sustainability (IPSOS), a groundbreaking initiative by Universiti Malaysia Terengganu (UMT), Malaysia and Beibu Gulf University (BGU), China, aims to foster cutting-edge research and collaboration in marine science, fisheries, and aquaculture. Leveraging the strengths of both institutions in exploring and conserving the marine resources of the South China Sea, IPSOS offers a unique platform for postgraduate students and researchers to enhance research frameworks, engage in effective communication, and produce impactful collaborative publications. This symposium not only signifies the solid partnership between UMT and BGU, as highlighted by their Memorandum of Understanding, but also serves as an essential 'sandbox' for innovative ideas and deep networking among Asia's brightest minds in marine sciences.