2017 MONAT Products & Clinical Studies Catalog

EYE WONDER LASH EYE WONDER LASH & BROW SERUM
& BROW ENHANCING SERUM

10 ml /.33 fl. oz.
Retail Price: $ 59.00 (USD) / $79.00 (CAD)

Prepare to Be EYEdolized!

Eye Wonder is a high-performance serum formulated with clinically
proven ingredients such as Capixyl™, which helps increase the
strength and condition of brows and lashes. It also contains Fision
KeraVeg18, a vegetable-based alternative to animal keratin which
helps strengthen and improve the luster of lashes and brows.

MP Price VIP Customer Price
$41.00 (USD) / $55.00 (CAD) $50.00 (USD) / $67.00 (CAD)

DIRECTIONS KEY INGREDIENTS

LASHES CAPIXYL™

Apply a thin layer of product twice FISION KERAVEG 18
daily on makeup-free skin along the
base of the upper and lower lash line INGREDIENTS LISTING:
(where eyelashes meet the skin). For Water, Hydroxyethylcellu-
maximum results, apply in the lose, Butylene Glycol, Trifoli-
morning and in the evening during 28 um Pratense (Clover) Flower
consecutive days. Extract, Acetyl Tetrapep-
tide-3, Tocopherol, Wheat
BROWS Amino Acids, Soy Amino
Acids, Arginine HCl, Serine,
Apply twice daily on makeup-free skin Threonine, Dextran, Benzyl
using short upward diagonal strokes Alcohol, Ethylhexylglycer-
on the eyebrow and use tip of finger to in.nus Officinalis (Rosemary)
dab in excess and aid absorption. For Leaf Oil, Salvia Hispanica
maximum results, apply in the Seed Extract, Tocopherol,
morning and in the evening during 28 Cinnamidopropyltrimonium
consecutive days. Chloride, Dextran, Butylene
Glycol
Allow product to dry for 7-10 minutes
before applying makeup.

OUR NOS • Phthalates • Parabens
• PEG • Harsh salt systems
Does Not • DEA/MEA • Harmful colors
Contain • Sulfates • Harmful fragrances

To learn more about Eye Wonder Lash & Brow Enhancing Serum, please contact your MONAT Market Partner.

SKU: 1017200CC0

S3 BY MONAT S3 SUPPLEMENT SUPPORT SYSTEM

Advanced nutrition for beautiful hair, because it’s yours!

60 capsules

Retail Price: : $59.00 (USD) / $79.00 (CAD)

PREMIUM INGREDIENTS+PREMIUM QUALITY = PREMIUM RESULTS
Get ready to take your hair growth to the next level
with our unparalleled Supplement Support System – S3 by MONAT

WHY S3? MONAT has raised the bar in hair nutrition with the supreme proprietary
blend of S3. Each of the clinically proven ingredients work together synergistically
to replenish the system of possible nutrient deficiencies that could be contributing
to hair loss. S3 provides a holistic hair regrowth treatment that works to promote
enhanced hair growth from the inside out.

Unleash the power of S3 with 1, 2, 3:
1. Exclusive Proprietary Blend
2. Clinically Proven Ingredients
3. 100% Drug Free

MP Price VIP Customer Price
$41.00 (USD) / $55.00 (CAD) $50.00 (USD) / $63.00 (CAD)

BENEFITS AND KEY INGREDIENTS SUPPLEMENT FACTS

An all-natural hair growth supplement. Amount per serving % Daily Value
OptiMSM®
A rich source of sulfur and a key component of collagen and keratin that Vitamin A (as beta-carotene) 8,000 IU 160%
supports the structural integrity of the hair and skin. 100%
ACTIVAloe® Vitamin C (as ascorbic acid) 60 mg 133%
Improves the elasticity of both the hair and skin. Helps to improve the 118%
appearance of aging skin and hair. Vitamin B1 (as thiamine hydrochloride) 2 mg 100%
EVNol™ 100%
May promote hair growth and increase the amount of hair in individuals Riboflavin (vitamin B2) 2 mg
experiencing hair loss. 50%
Biotin Niacinamide 20 mg 833%
provides structural support to the hair shaft and follicle. 1,667%
Vitamin A (as beta-carotene) Vitamin B6 (as pyridoxine hydrochloride) 2 mg 100%
Counteracts Vitamin A deficiency which is common in people who are
experiencing hair loss. Folate (as folic acid) 200 mcg 67%
Zinc 36%
Contains anti-aging properties which are beneficial to hair health. Vitamin B12 (as cyanocobalamin) 50 mcg 50%

SUGGESTED USE Biotin 5000 mg *

Take (2) capsules daily, preferably with a meal or as Pantothenic acid (as d-calcium pantothenate) 10 mg
directed by your healthcare practitioner.
Zinc (as amino acid chelate) 10 mg

Selenium (as selenium amino acid chelate) 25 mcg

Copper (as copper amino acid chelate) 1 mg

Proprietary Blend: 900 mg

OptiMSM® (methyl sulfonyl methane)
ACTIValoe® (Aloe Vera extract 200:1) (Aloe barbadensis) (leaf)
EVNol™ Natural d-Mixed Tocotrienols (d-alpha, beta gamma & delta tocotrienols)

* Daily Value not established

Other ingredients: Hypromellose, rice flour, silicon dioxide and vegetable magnesium stearate.

**These statements have not been evaluated by the Food and Drug Administration.
This product is not intended to diagnose, treat, cure or prevent any disease.
***Pregnant or lactating women should consult a physician before taking this product.

OptiMSM® is a registered trademark of Bergstrom Nutrition.
ACTIValoe® is a registered trademark of Aloecorp Inc.
EVNol™ is a trademark of ExcelVite Inc. and protected by US Patent No-7,211,274.

OUR NO’S • Gluten • Sulfates Our clinically proven 64% of subjects showed
• GMOs • Parabens ingredients have
Does Not • Animal byproducts • Harsh salt systems demonstrated the regrowth of between 10-35%
Contain • Phthalates • Harmful colors
• PEG • Harmful fragrances following outcomes: 27% had 50% greater
• DEA/MEA
hair regrowth

To learn more about S3 by MONAT, please contact your MONAT Market Partner.

HAIR QUIZ No hair treatment is complete without MONAT's
REJUVENIQE™ Oil Intensive and S3 Supplement Support
CHEAT SHEET System!

1. Choose your desired outcome REJUVENIQE™ helps reduce hair thinning and prevent oxidative
from the RESULTS column. stress while adding long-lasting shine.
2. Moving across the chart, line up the
desired result with the CONCERN you S3 provides advanced nutrition for your hair and promotes enhanced
wish to address. hair growth from the inside out.
3. Get your ideal four-product combo,
from the RECOMMENDED columns. Make sure to include these two essential products as part of your
everyday hair care regimen!

RECOMMENDED CONCERN RECOMMENDED CONCERN RECOMMENDED CONCERN RECOMMENDED CONCERN RECOMMENDED CONCERN RECOMMENDED CONCERN RESULTS VOLUME HYDRATION SMOOTH SHINE TEXTURE

DRY SCALP IRT Shampoo IRT Shampoo
IRT Conditioner IRT Conditioner
IRT Shampoo IRT Shampoo IRT Shampoo Intensive Repair Treatment Intensive Repair Treatment
IRT Conditioner IRT Conditioner IRT Conditioner Restore Leave-In Conditioner Black Groom Styling Clay
Intensive Repair Treatment Intensive Repair Treatment Intensive Repair Treatment
Restore Leave-In Conditioner Restore Leave-In Conditioner Restore Leave-In Conditioner Renew Shampoo Renew Shampoo
Replenish Masque Replenish Masque
DAMAGED HAIR Revitalize Conditioner Revitalize Conditioner
Restore Leave-In Conditioner Restore Leave-In Conditioner
Revive Shampoo Renew Shampoo Renew Shampoo
Replenish Masque Replenish Masque Replenish Masque IRT Shampoo IRT Shampoo
Revitalize Conditioner Revitalize Conditioner Revitalize Conditioner IRT Conditioner IRT Conditioner
Restore Leave-In Conditioner Restore Leave-In Conditioner Restore Leave-In Conditioner Intensive Repair Treatment Intensive Repair Treatment
Black Groom Styling Clay Black Groom Styling Clay
THINNING HAIR
Revive Shampoo Revive Shampoo
IRT Shampoo IRT Shampoo IRT Shampoo Replenish Masque Replenish Masque
IRT Conditioner IRT Conditioner IRT Conditioner Revitalize Conditioner Revitalize Conditioner
Intensive Repair Treatment Intensive Repair Treatment Intensive Repair Treatment Restore Leave-In Conditioner Restore Leave-In Conditioner
Black Groom Styling Clay Black Groom Styling Clay Black Groom Styling Clay
Renew Shampoo Renew Shampoo
HAIR BREAKAGE Revitalize Conditioner Revitalize Conditioner
Restore Leave-In Conditioner Restore Leave-In Conditioner
Revive Shampoo Revive Shampoo Revive Shampoo ReStyle Instant Sculpting Taffy ReStyle Instant Sculpting Taffy
Replenish Masque Replenish Masque Replenish Masque
Revitalize Conditioner Revitalize Conditioner Revitalize Conditioner Renew Shampoo IRT Shampoo
Restore Leave-In Conditioner Restore Leave-In Conditioner Restore Leave-In Conditioner Revitalize Conditioner IRT Conditioner
Restore Leave-In Conditioner Tousled Texturizing Mist
FRIZZY HAIR ReFinish Control Hairspray Black Groom Styling Clay

Renew Shampoo Renew Shampoo Renew Shampoo
Revitalize Conditioner Revitalize Conditioner Revitalize Conditioner
Restore Leave-In Conditioner Restore Leave-In Conditioner Restore Leave-In Conditioner
ReStyle Instant Sculpting Taffy ReStyle Instant Sculpting Taffy ReStyle Instant Sculpting Taffy

OILY SCALP/HAIR

BLACK 2-in-1 Shampoo + Conditioner Renew Shampoo IRT Shampoo
Replenish Masque Revitalize Conditioner Revitalize Conditioner
Reshape Root Lifter Reshape Root Lifter Restore Leave-In Conditioner
ReFinish Control Hairspray Black Groom Styling Clay Black Groom Styling Clay

MONAT
HAIR CARE TIPS

1.Brush your hair vigorously before you 6. When using a towel to dry your hair, press
shower to prevent tangles and to stimulate onto wet hair and scalp gently. Hair is most delicate
the scalp.
when wet so don’t use rough rubbing actions to dry with a
Stimulating your scalp increases blood flow and circulation, towel. Wrap well and allow water to be absorbed.
which benefits the hair follicles and hair. Increased blood
flow will make for a healthier scalp, healthier hair and 7. Get the full benefits of your styling products
support hair growth. by applying them 2 minutes before you style
to let them fully absorb into your strands.
2. Wet hair thoroughly when washing.
After combing your hair out of the shower, towel dry as
Hair should be thoroughly wet before adding shampoo, like needed then apply your products right away and let them
MONAT Renew Shampoo. Warm water will open the absorb into your strands for about 2 minutes before you pick
cuticle, which will help remove any dirt or build-up trapped up your hair dryer and other styling tools. Special note:
in the hair. Rinse out conditioner with cool water which When using TOUSLED Texturizing Mist in your hands, rub
helps close the hair cuticle. together then distribute throughout your hair.

3. Shampoo your hair twice for best results. 8. Dry before you style.

The first wash removes excess dirt and build-up. The Before using your curling iron or flat iron, make sure you hair
second wash gets your hair clean. Use the second shampoo is completely dry. Placing a heating tool on wet or damp hair
to work shampoo into the roots and create a proper lather. can cause slow damage to your hair.
Keep in mind that MONAT shampoos are formulated to
remain on the scalp for at least two minutes to allow 9. Tame flyaways by using some MONAT
ingredients to be absorbed. RESTYLE Instant Sculpting Taffy or
REJUVENIQETM Oil Intensive.
4. To prevent hair breakage, only use a
wide-tooth comb or wet brush when hair is Combat those little hairs that won’t that won’t stay flat by
wet to detangle your hair. using RESTYLE Instant Sculpting Taffy on dry hair. You can
also put a few drops of REJUVENIQETM Oil Intensive in your
The comb and wet brush don’t tug and pull at your hair like hands, rub together and distribute on those flyaways.
a regular brush so they safely and easily remove tangles
with less damage. 10. What is dry shampoo and how do I use it?

5. Shake it! The CHAMP Conditioning Dry Shampoo by MONAT works
by absorbing oil from your scalp and hair, making it look and
Because MONAT products contain many natural feel freshly washed. Spray at the roots as needed, massage
ingredients, it is best to shake the products well before it in with your fingers and then brush. Tip: Always shake the
using. Especially the REFINISH Control Hairspray, shampoo well before utilizing.
RESHAPE Root Lifter, TOUSLED Texturizing Mist and THE
CHAMP Conditioning Dry Shampoo.

MONAT TREATMENT SYSTEMS
CLINICAL STUDIES

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MONAT TREATMENT SYSTEMS CLINICAL STUDIES

These independent clinical studies were conducted at top manufacturing and research
centers. These facilities approach hair care from a fundamental science perspective; closely
examining the basic structure of hair, of hair care products, and interactions between the two
from an ingredient position. Examiners within the facilities look to gain knowledge and
understanding of the principles of hair and hair product interactions.

The results of these studies support MONAT’s proprietary blend of naturally-based active
biofunctional ingredients. No serious adverse side effects were reported from the
treatments; instead, the clinical studies show that MONAT ingredient users experienced the
following benefits:

• Significant hair growth with an average increase in hair count per sq. in.
• Decrease of the thinning process
• Increased density and fullness
• Substantial decrease in hair fallout
• Increased rate of hair growth
• More manageability of the hair
• Overall better shine and condition of hair

MONAT TREATMENT SYSTEMS CLINICAL INGREDIENTS.

Studies were scheduled and held independently utilizing one active ingredient per study.
These ingredients included:

• Capixyl™
• PROCATALINE™ ™
• Crodasorb™

CAPIXYL™

Objective
The goal of the study was to evaluate a solution containing Capixyl™ concentration.

It was impartially evaluated by using instrumental measurements (digital trichogram with
TrichoScan™). Measurements from a group of treated volunteers were compared with those

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of Placebo-treated ones. Each volunteer used the product on a daily basis. The volunteers
were chosen using the following conditions: 200 hairs or less on digital trichogram hair
count, and/or less than 70% hairs in the growing phase.

Volunteer Protocol
• Volunteers suffering from hair loss (average age 30-46).
• Volunteers were clinically evaluated and individual case histories were recorded in order to
rule out possible abnormalities due to outside influences affecting ideal results.
• Products were applied in the evening and distributed on the testing area during 4
consecutive months.
• Every week, volunteers were given a plastic bag, where they had to collect all the hairs on
their pillows, combs and clothes on a daily basis; they had to bring the bag to the laboratory
for hairs to be counted.

Methodology of Trichoscan
TrichoScan is appropriate for the analysis of human scalp hair. The most important benefits
are:
• Total hair counts can be analyzed within the same day.
• The same target site can be used to calculate the number of growing and non-growing
hairs.

Determination of total hair density
1. A shaving mask is positioned on the volunteer head
in order to shave a 1.8 cm2 area on the zone or zones
to be studied.
2. Images were recorded with the equipment camera
in order to evaluate the growing and non-growing
phases. Volunteers were asked to not wash their hair
for two days prior to the evaluation with TrichoScan.

Recording the images
After capturing, the digital images are transferred to a specific software for the analysis of the
total hair density (growing + non-growing).

1. Original image
2. Detection of hair
3. Specific discernible hair
4. Detection of hair in growing and non-growing phases:

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• Red: non-growing phase
• Green: growing phase
• Yellow: resting phase

RESULTS
Determination of growing hair count
The TrichoScan software defines growing hair based on the knowledge that hair grows at
approximately 0.3 mm/day whereas non-growing hairs do not grow consistently. During
successful testing, the growing hair count should increase and therefore this approach can be
used to monitor a true response.
Determination of non-growing hair count
The software measures the length of hair and, by statistical analysis, discriminates between
growing versus non-growing hairs.
The study demonstrates a clear 46% increase in the growing hair density of volunteers
having used a solution containing Capixyl™ concentration in comparison with placebo after 4
months application.
The study demonstrates improvement in repair effect improving hair anchoring by 70%.
The study demonstrates a substantial reduction in DHT hormone, resulting from the action of
5a-reductase on testosterone by 48%.
The study also demonstrates a strong reduction in the non-growing hair density in volunteers
having used a solution containing Capixyl™ concentration in comparison with the placebo
after 4 months application.

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Before & After Pictures

Irritation & Sensitization Patch Test
Objective
This test was done to determine the absence of irritation and sensitivity tendencies following
repeated skin applications of a solution containing the ingredient CAPIXYL™ with a patch test.
This test is widely recognized to evaluate skin sensitivity and allergenic reactions.
Study Significance
Skin allergy is an occurrence of immune origin that transpires according to three phases:
• Close contact of a foreign allergenic substance with the skin
• Sensitivity of the immune system following this first contact
• Activation of immune reactions following a second exposure of the skin to the allergen
All 3 steps are required to document the allergenic potential of a given substance
Protocol
Test Group:
• Number of subjects: 112 volunteers, women and men, 18 to 84 years old.
Test application:
• Areas: on the back of each subject.
• Quantity: CAPIXYL™
• Frequency and duration:

o Induction period: 3 weeks
o Rest period: 1 week
o Challenge phase: 1 week

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Test Criteria
After each application, the patch is removed and the clinical examination is performed by the
technician 30 minutes later.

The result of examination is negative if the skin looks normal.

The clinical examination is made on the back using the following criteria and scale (quotation
0 to 4):

Score Response Visible Change

0 Absent None
faint, redness with poorly defined margins
1 Inflammation
vascular Redness with well defined, margins
dilation Redness plus well defined edema

2 Redness plus papules, or vesicles or ulceration

3 Inflammation
infiltration

RESULTS
Initial test:
No responses were noted on any of the 112 subjects who underwent at least one
post-application examination. The absence of responses characterizes the product as one
which is devoid of clinically significant skin-irritating tendencies.

Challenge test:
Original contact sites: no responses were noted on any of the 105 subjects who participated
in this phase of the study. The absence of responses illustrates the product as one which is
devoid of clinically significant skin sensitizing tendencies.

CONCLUSION
No significant dermal reactions were exhibited during either the induction phase or challenge
phase of the study.

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PROCATALINE™
Objective
The goal of the study was to evaluate a solution containing PROCATALINE™ concentration.

It was impartially evaluated by using instrumental measurements (digital trichogram with
TrichoScan™). Measurements from a group of treated volunteers were compared with those
of Placebo-treated ones. Each volunteer used the product on a daily basis. The volunteers
were chosen using the following conditions: 200 hairs or less on digital trichogram hair
count, and/or less than 70% hairs in the growing phase.

Methodology
Efficacy tests performed Ex vivo evaluation

UV damage
Hematoxylin and Eosin staining
Immuno-fluorescence staining

H2O2 damage
Hematoxylin and Eosin staining
Immuno-fluorescence staining

Melanin
Fontana-Masson staining

Material
• 6 mm punch biopsies of scalp skin
• Application: PROCATALINE™ biofunctional
• Stress: 5J/cm² UVA + 200mJ/cm² UVB Method
• Scalp skin treated with PROCATALINE™ biofunctional for 24h, then stressed with UV and
kept in culture 24h again
• Punch biopsies were embedded in paraffin or in OCT and frozen in liquid nitrogen
• Hematoxylin and Eosin staining
• Immuno-fluorescence staining
• Detection by fluorescence microscopy

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PROCATALINE™ biofunctional helps maintain P63 expression in hair follicle under UV
stress, ex vivo.

Control (without UV) UV Control PROCATALINE™
biofunctional 1% + UV

PROCATALINE™ biofunctional treatment before UV exposure significantly maintained P63
expression. This helps to create a favorable environment to promote hair growth.

PROCATALINE™ biofuntional helps maintain high level of anti-oxidative enzyme, ex
vivo.

Control PROCATALINE™
(without STRESS) biofunctional 1% + UV

PROCATALINE™ biofunctional helped boost the expression of catalase enzyme. Hair
follicle appears better preserved when exposed to UV irradiation.

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PROCATALINE™ biofunctional helps limit cell death in hair follicle, ex vivo.

Control (without H2O) H2O CONTROL PROCATALINE™
biofunctional 1% + H2O2

No caspase-3 staining was observed in the PROCATALINE™ biofunctional-treated follicle
suggesting reduced cell death.

RESULTS
As shown by ex vivo studies, PROCATALINE™ biofunctional treatment before UV exposure
may help to:
• Create an environment favorable to hair renewal and growth
• Preserve hair cells and increase the hair follicle strength by 35%
• Preserve hair follicle and structure from H2O2 damage
• Increase collagen production, directly increasing follicle size by 70%
• Preserve melanin in hair follicle under oxidative stress

Irritation & Sensitization Patch Test
Objective
This test was done to determine the absence of irritation and sensitivity tendencies following
repeated skin applications of a solution containing the ingredient PROCATALINE™
concentration with a patch test.
This test is widely recognized to evaluate skin sensitivity and allergenic reactions.

Study Significance
Because human skin functions as a physicochemical barrier, molecules over 500 Daltons of
molecular weight are usually unable to traverse the stratum corneum. Penetration of
proteins/polypeptides through normal skin is extremely low and normally without

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consequence. Proteins/peptides are not expected to interact directly with DNA or other
chromosomal material. The protein extract of pea is not expected to either have genotoxicity
potential or cause systemic toxicity by dermal exposure and no systemic toxicity studies are
needed. Therefore, PROCATALINE™ is safe to use as a cosmetic ingredient at the
recommended use level.

Dermal Irritation
PROCATALINE™, tested as supplied, was non-skin irritants, based on the in vitro Skin
Irritation Test (SIT) Using the Epiderm™ Skin Model.

The skin irritation test using the EpiDerm™ skin model met the requirements of the ŒCD
TG439 guideline and was conducted in full compliance with the GLP (Good Laboratory
Practice) regulations. Three EpiDerm™ skin tissues were topically exposed to the
PROCATALINE™, as supplied, for 60 minutes. After 42-hour post-treatment incubation, the
EpiDerm™ skin tissue viability was determined by MTT
(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) conversion assay. The
relative viability of the test article-treated tissues was calculated and the skin irritation potential
was evaluated. Based upon the mean tissue viability of 94.0%, PROCATALINE™, as
supplied, was predicted to be non-skin irritants according to the prediction model validated
by ECVAM and to the ŒCD Test Guideline 439.

Ocular Irritation
PROCATALINE™, tested as supplied, had minimum ocular irritation potential based on the
Topical Application Ocular Irritation Assay Using the Epiocular™ Human Cell Construct.

The ocular irritation assay using the EpiOcular™ human cell construct was conducted in full
compliance with the GLP regulations. The EpiOcular™ tissues were topically treated with
PROCATALINE™ , as supplied, for 4, 8, 16, and 24 hours (2 tissues/ time point). The MTT
conversion assay was used to assess cellular viability. The ocular irritation potential was
evaluated by the duration of exposure required to reduce the EpiOcular™ tissue viability to
50% of controls (ET50). PROCATALINE™ showed insignificant cytotoxicity at all exposure
times and the ET50 was determined as greater than the longest exposure time tested (ET50
> 24 hours). The results of the assay indicated that PROCATALINE™, as supplied, had
minimum ocular irritation potential.

RESULTS
Repeated Insult Patch Test (RIPT)
In an RIPT completed by 214 human subjects, PROCATALINE™ demonstrated no potential
for dermal irritation or allergic contact sensitization.

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Each of 214 human subjects received 0.2 ml of PROCATALINE™ as supplied on the upper
back area. Following a 24-hour exposure period, test patches were removed and sites scored
for erythema and edema. A series of nine induction patches was applied three times a week
for three weeks. Following a two-week rest period, challenge patches were applied to a virgin
site on the back and allowed to remain in skin contact for 24 hours. Challenge sites were
scored for erythema and edema at 24 and 72 hours post patching. No significant dermal
reactions were exhibited during either the induction phase or challenge phase of the study.

CONCLUSION
No significant dermal reactions were exhibited during either the induction phase or challenge
phase of the study.

CRODASORB™
Objective
The goal of the study was to evaluate the effect of UV Radiation on hair with CODASORB™
concentration.

CRODASORB UV-283 has been shown to preserve the natural color of the hair. The fact
that brown tresses do not lighten in color indicates that CRODASORB UV-283 prevents
UV-B radiation from degrading melanin, the pigment that gives hair its color. During testing it
was also discovered that both blond and gray tresses treated with CRODASORB UV-283
retained their natural color after UV-B exposure. This same color protection was not
observed in the untreated tresses or in the tresses treated with Octyl Methoxycinnamate;
blond hair tresses turned a brassy gold and gray hair tresses appeared to yellow. Such
adverse color changes are an indication of Tryptophan degradation.

Methodology
Combing Studies

CRODASORB UV-283 prevents the photodamage that causes hair to develop undesirable
combing properties after UV exposure, as seen in studies evaluating its effect on the
combing work of tresses exposed to UV radiation. One study found that CRODASORB
UV-283 reduced the work of combing by 58% in virgin hair and by 33% in bleached hair
compared to the untreated, UV-exposed sample, a clear indication that CRODASORB
UV-283 has prevented photodamage.

The combing study consisted of two parts, an independently run exposure phase and an
in-house testing phase. During the exposure phase, treated and untreated tresses of virgin

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and bleached hair underwent 29 days of UV-A/UV- B exposure at 375.8 Joules/day.
Untreated and unexposed tresses were used as controls. During the second phase of in-
house testing, wet combing measurements for each of the sample tresses were obtained
using the Dia-Stron tensile tester. These measurements were used to compare the combing
work of UV-exposed tresses treated with CRODASORB UV-283 with that of UV-exposed
tresses left untreated. The bar chart shown on the following page depicts these combing
measurements. Values are expressed as total combing work (Joules) and represent absolute
numbers, not percent reductions.

CRODASORB UV-283 Reduces Combing Work After UV Exposure

Additional Studies
Tryptophan analysis, contact angle
measurements, and fiber breakage
testing all confirm that
CRODASORB UV-283 prevents
UV-B damage in hair and helps
maintain the integrity of both the
cuticle and the cortex. Prior to
testing, treated and untreated hair
tresses were placed in a
temperature/humidity-controlled
chamber set at 27°C and 65% RH
and exposed to UV radiation using
UV-B lamps which emitted
0.0025 Watts/cm², which is the
equivalent of 8 times the daily UV
exposure at Mauna Loa, HI.

Treatment:
Hair tresses were grouped into one of four categories and then treated as described below
during the UV exposure period. Twenty hair fibers from each group were withdrawn for
testing at Days 3, 6 and 12.

A. No treatment/No exposure to UV
B. Washed every 24 hours with solution containing CRODASORB UV-283
C. Washed every 24 hours with solution containing Octyl Methoxycinnamate
D. Washed every 24 hours with solution containing no UV absorber

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Spectrofluorometric Analysis of Tryptophan
Tryptophan is an amino acid found in the hair. Since it decomposes on exposure to UV
radiation, Tryptophan can be used as a marker for UV damage. Spectrofluorometric analysis
shows that the peak for Tryptophan in unexposed hair is between 300 and 350nm (Sample
A in the graph shown below). As the curves indicate, CRODASORB UV-283 (Sample B) is
effective in preserving Tryptophan, while the other UV-B exposed tresses (Samples C, D, D1,
D2) show complete degradation of the amino acid.

RESULTS
Laboratory results indicate that CRODASORB UV-283 exhibits significantly higher
substantivity in the absence of anionic surfactants.
• CRODASORB UV-283 Reduces Combing Work After UV Exposure
• CRODASORB UV-283 is effective in preserving tryptophan, which when degraded is an
indicator of UVB damage
• CRODASORB UV-283 Keeps Hair Stronger After UV-b Exposure

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• CRODASORB UV-283 has not only prevented damage to the cuticle, but has also
provided conditioning benefits.
• CRODASORB UV-283 helps protect gray hair from the sun by preventing UV-B damage
at the surface.
• CRODASORB UV-283 means the hair has maintained its elasticity and is less brittle.
• CRODASORB UV-283 protects the structural integrity of gray hair exposed to UV
radiation.

Irritation & Sensitization Patch Test
Objective
An Epi-Derm study was used to assess the acute irritation potential of Crodasorb UV-283.
Composition details: Crodasorb UV-283-LQ-(MH) is a mixture Cinnamidopropltirmonium
Chloride and water.

Toxicological summaries for the components of the mixture.
1. Eye Irritation
2. Skin Irritation/Sensitization: Human Repeat Insult Patch Test
3. Skin Irritation: EpiDerm

RESULTS
Concentration Average Score

Distilled Water (100%) 1.75
Crodasorb UV-283

2. Skin Irritation/Sensitization – Human Repeat Insult Patch Test
An aqueous solution of Crodasorb UV-283 was evaluated to determine whether it would
induce primary or cumulative irritation and/or allergic contact sensitization. The induction
phase consisted of applying a 1 in. by ¾ in. semi-occluded patch containing 0.2 mL of the
test article to the upper back of 56 human test subjects. After a 24-hour exposure period, the
patch was removed and examined for signs of irritation. After a 24-hour rest period, the test
was repeated and continued 3 times a week for 3 weeks. Upon completion of the induction
phase and a two-week rest period, the test subjects were challenged with a single application
of the test article adjacent to the original test site. After 24 hours, the patch was removed. The
test site was examined for signs of sensitization 24 and 72 hours post application.

There were no visible skin reactions on any of the test subjects throughout the testing
interval. Under the conditions of this study, Crodasorb UV-283 aqueous solution did not
indicate a potential for dermal irritation or allergic contact sensitization.

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3. Skin Irritation
Crodasorb UV-283 was evaluated for corrosive potential utilizing the Mat Tek Corporation
EpiDerm in vitro toxicity testing system. After the appropriate tissue preparation, 100 uL of
the negative control article were added to the millicells containing the EpiDerm samples that
were incubated at 37°C 5% CO2 and >90% humidity. After the appropriate exposure periods
(1hr, 4.5hrs, 20hrs), each insert was individually removed from its plate and rinsed with
phosphate buffered saline (PBS) to remove any residual material. Each was then rinsed a
second time. Excess liquid was shaken off and each EpiDerm sample was placed into 300
microliters of MTT solution. The Epiderm samples were then returned to the incubator. After
the 3 hour MTT exposure, each insert was removed and gently rinsed with PBS to remove
any residual MTT solution. Excess PBS was shaken from each of the inserts, which were
then blotted on the bottom using paper towels. The inserts were then each placed into 1 well
of a 24 well extraction plate. Each insert was then immersed in 2 mL of extraction solution
overnight. After the exposure the liquid was decanted back into the original well, agitated and
a 200 uL aliquot was removed for evaluation.

Toxicological Assessment for Crodasorb UV—283
An automatic microplate reader was used to determine the absorbance of each extract at
570nm. The percent absorbance of the test and reference articles that were determined with
the negative control was defined as 100%. According to the MatTek Corp. as a general
guideline the following groups can be used in assuming expected in vivo irritancy response
based on ET-50 results from the EpiDerm.

ET-50 (hours) Expected in vivo irritancy
< 0.5 = Severe, probably corrosive
0.5-4 = Moderate
4-12 = Moderate to mild
12-24 = Very mild
24 = Non-irritating

The Crodasorb UV-283 elicited and ET-50 of approximately 8.0 hours as per the above
chart Crodasorb UV-283 has an in vivo dermal irritancy potential of moderate to mild.

CONCLUSION
No significant dermal reactions were exhibited during either the induction phase or challenge
phase of the study.

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(Extended Version)

REJUVENIQE™
Objective
The goal of the study was to evaluate a solution containing REJUVENIQE™ with Abyssinian
Oil.
Many claims are being made to demonstrate the effectiveness of hair care products. These
include aspects like hair manageability, protection, strengthening of the hair strands and
anti-breaking properties. Furthermore a visible benefit like the enhancement of the natural
shine of the hair is important to achieve customer satisfaction.

Volunteer Protocol
The tests were performed on Mulatto and Caucasian hair. Mulatto hair offers a combination
of characteristics of different ethnic hair types mixed with African origin. Due to the ellipticity
and high degree of curliness hair of African or Mulatto origin has a tendency to be very
sensitive against damage. Therefore, an improved hair quality in this type of hair can
demonstrate the benefit of a treatment with REJUVENIQE with Abyssinian Oil very efficiently.
Caucasian hair can be characterized as having straight hair texture which is resistant to
damage, but has a duller appearance.

RESULTS
Dry Combing Test
A repeated grooming test was performed to quantify the shine of the hair. The hair tresses
were cleansed with a non-conditioning shampoo, dried overnight under controlled humidity
(60%) and afterwards the oils were applied to the hair.

Based on the results of three different test methods we can claim that REJUVENIQE™ with
Abyssinian Oil offers a high benefit in improving the manageability of the hair, enhancing the
shine and strengthening the hair strands. It can be claimed as being a natural replacement to
synthetic ingredients in hair care products like silicones, where shine, luster and a
strengthening effect is needed.

Dry Combing Mulatto and Caucasian Hair

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QuantificationUonftSrehainteed Treated Untreated Treated

Objective

The shine of the hair is a very highly appreciated attribute of healthy-looking hair and is often

a claim of hair care products.

This test is widely recognized to evaluate luster and shine with light reflecting properties.

Protocol
The commercially-available SAMBA device by Bossa Nova was used to quantify the shine
enhancement of REJUVENIQE™ with Abyssinian Oil. This method was developed to
measure the luster and shine of the hair tress by light reflected from a curved hair tress. The
quantification is based on an image analysis by scanning the light distribution of a hair
sample across highlighted and dark areas. The resultant image was evaluated by using
image analysis software with developed macros. In general the hair pigmentation has a
significant effect on the optical properties of hair and on its luster. Hair with low pigmentation
reflects the light which results in a broad light distribution. The luster is calculated as a
function of light intensity and the distance along the hair tress, therefore dark hair has the
narrowest light distribution. This results in increasing luster for increasing pigmentation of the
hair. To ensure statistical relevance eight tresses were evaluated per sample. The Mulatto
Hair that was used was non-chemically modified, the Caucasian Hair was bleached once.

SAMBA device tester
RESULTS
The natural shine of untreated Mulatto Hair is around 165 technical shine units (in Reich-
Robbins units). The effect of using REJUVENIQE™ with Abyssinian Oil to enhance the shine
of the hair is impressive by almost doubling the apparent shine on the Mulatto hair in
comparison to untreated hair up to 289 units. Typical values for bleached Caucasian hair by
this method are around 18 – 20 units, which corresponds to a much duller initial state for this

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MONAT TREATMENT SYSTEMS
CLINICAL STUDIES
(Extended Version)

hair structure.

REJUVENIQE™ with Abyssinian Oil has
the ability to increase the shine of
Caucasian hair by 88%, which is more
than 4.4 fold of the initial shine. Due to
these results it can be claimed, that
REJUVENIQE™ with Abyssinian Oil is a
natural shine enhancer. It can be used
as a natural replacement to synthetic oils
like silicones in Hair Care products where shine and luster is needed.

CONCLUSION
REJUVENIQE™ with Abyssinian oil is a natural hair conditioning agent with numerous
benefits. The great performance of REJUVENIQE™ with Abyssinian oil for hair care
applications have been displayed in the test results presented in this report. It increases the
manageability of the hair, strengthens it and makes is more resistant against external stress
like grooming with an anti-breakage effect. Furthermore it is a very effective shine enhancer
while being non-greasy. These benefits can be seen on different hair structures like Mulatto
and Caucasian Hair. Therefore it can be used as a natural replacement for other oils used in
hair care applications and synthetic ingredients like silicones and mineral oil.

Conditioning Effect
REJUVENIQE™ with Abyssinian oil increased the combability both on Mulatto and
Caucasian hair. The positive effect is even more obvious on the kinky Mulatto hair, which is
known to be more difficult in styling.

Anti-Breakage Effect
The repeated grooming test showed the capability of REJUVENIQE™ with Abyssinian oil to
strengthen the hair and significantly reduce the breaking of hair fibers. Especially on the more
sensitive and easily breaking Mulatto hair, REJUVENIQE™ with Abyssinian oil offers a
dramatic benefit even outperforming the effect on Caucasian Hair.

Natural Shine Enhancer
The shine enhancing capability of REJUVENIQE™ with Abyssinian oil can be impressively
demonstrated on both hair types, which had been evaluated and can almost double the shine
of Mulatto hair, and the shine of initially duller Caucasian hair can be enhanced by 88%