C 2329-9541-InfectiousDiseases-2015_Posters-Accepted Abstracts

323rd OMICS International Conference August 10-12, 2015 London, UK

World Congress on

Infectious Diseases

Accepted Posters

Infectious Diseases-2015
Page 162

Woo Young Choi et al, J Immunol Tech Infect Dis 2015, 4:2
http://dx.doi.org/10.4172/2329-9541.C1.003

World Congress on

Infectious Diseases
August 10-12, 2015 London, UK

Monoclonal antibodies against envelope glycoprotein of yellow fever virus

Woo Young Choi, Seok-Min Yun, Eunbyeol Wang, Young Eui Jeong, Myungguk Han and Won-Ja Lee
Korea Centers for Disease Control & Prevention, Republic of Korea

Yellow fever virus (YFV) belongs to family Flaviviridae and is important mosquito-borne human pathogens. In this study,
we describe the generation of monoclonal antibodies (mAbs) against envelope glycoprotein (E) of 17D Yellow fever
vaccine virus for serologic diagnosis. After immunization of BALB/c mice with recombinant E protein, a total of 5 monoclonal
antibodies were generated from the spleen of mice. Immunoglobulin types of antibodies were found to be 1 IgG2a/kappa,
2 IgG2b/kappa, and 2 IgG1/kappa. Indirect immunofluorescence assay revealed that all of the mAbs were reactive to YFV-
infected cells. These data suggest that 5 monoclonal antibodies can be used for detection of YFV and have the potential for use
in serodiagnosis.

[email protected]

Notes:

J Immunol Tech Infect Dis 2015 Infectious Diseases-2015 Volume 4 Issue 2
ISSN: 2329-9541, JIDIT Hybrid Open Access August 10-12, 2015 Page 163

Won-Ja Lee et al, J Immunol Tech Infect Dis 2015, 4:2
http://dx.doi.org/10.4172/2329-9541.C1.003

World Congress on

Infectious Diseases
August 10-12, 2015 London, UK

Genome sequencing and analysis of SFTSVs isolated from patients in South Korea during 2013

Won-Ja Lee1, Sun-Whan Park1, Mi-ran Yun2, MyunggukHan1, Dae-Won Kim2 and WooYoung Choi1
1Division of Arboviruses
2Division of Biosafety Evaluation and Control
3Korea Centers for Disease Control & Prevention, Republic of Korea

Severe fever with thrombocytopenia syndrome (SFTS) is a new emerging infectious disease in China, Japan, and South
Korea. It is caused by SFTS virus (SFTSV), in the genus of Phlebovirus (family Bunyaviridae). The major clinical symptoms
and laboratory parameters of SFTS are fever, thrombocytopenia, leukopenia, and elevated serum hepatic enzymes, and SFTS
patients usually die due to multiple organ failure. In this study, we conducted the complete or draft genome sequencing of
SFTSVs which were isolated from patients in South Korea during 2013. The single-stranded RNA genome comprised of three
segments: L, M, and S. All coding sequences for five complete SFTSV sequences (L, M and S segment) and ten draft sequences
(M and S) from Korean strains were nearly identical, with sequence similarities of 93.9%–100% for the S segment, 93.2%–99.8%
for the M segment, and 96.2-97.6% for the L segment. These strains were compared to strains from two neighboring Asian
countries, China and Japan. In phylogenetic analysis, the five Korean SFTSV sequences clustered with all of eight Japanese
and three of 102 Chinese (KF374682, KJ597825, JQ670934) strains on the L segment. On the M segment, 12 of 15 Korean
sequences grouped with all of eight Japanese and two of 106 Chinese (KF374684, KJ597824) isolates. All coding sequences will
be studied further to determine the precise phylogenetic features and the transmission pattern.

[email protected]

Notes:

J Immunol Tech Infect Dis 2015 Infectious Diseases-2015 Volume 4 Issue 2
ISSN: 2329-9541, JIDIT Hybrid Open Access August 10-12, 2015 Page 164

Wang Weiming, J Immunol Tech Infect Dis 2015, 4:2
http://dx.doi.org/10.4172/2329-9541.C1.003

World Congress on

Infectious Diseases
August 10-12, 2015 London, UK

Study of the first traditional Chinese medicine on the treating clinically Mycoplasma Pneumoniae

Wang Weiming
Heilongjiang Academy of Chinese Medicine Sciences, China

Qinbaiqingfei pellets is the first Traditional Chinese Medicine clinically used in treating Mycoplasma Pneumoniae
(M. pneumoniae) infections in China and consists of Scutellaria baicalensis, Platycodon grandiflorum and so on that
has long and widely been used in clinic for the treatment of various diseases including respiratory tract infections. Using
Quantitative Diagnostic Kit for Mycoplasma Pneumoniae DNA to illuminate Anti-M. pneumoniae effect of Qinbai in the cell
and experimental animal after M. pneumoniae infection. Using HE stains, MTT to determine the growth recovery effects of
the Qinbai. Our research results showed that Qinbai could significantly inhibit M. pneumoniae and promote cell growth after
anti- M. pneumoniae treatment in the infected cells or mice.

Biography

Wang Weiming obtained her Ph.D. from Heilongjiang University of the Traditional Chinese Medicine and postdoctoral studies from China Academy of Chinese
Medicine Sciences. She has worked in Heilongjiang Academy of Chinese Medicine Sciences as a researcher since 1998.

[email protected]

Notes:

J Immunol Tech Infect Dis 2015 Infectious Diseases-2015 Volume 4 Issue 2
ISSN: 2329-9541, JIDIT Hybrid Open Access August 10-12, 2015 Page 165

Meng Yan-li, J Immunol Tech Infect Dis 2015, 4:2
http://dx.doi.org/10.4172/2329-9541.C1.003

World Congress on

Infectious Diseases
August 10-12, 2015 London, UK

Establishing the model of proteomic analysis on the M. pneumoniae

Meng Yan-li
Heilongjiang Academy of Chinese Medicine Sciences, China

Qinbaiqingfei pellets are the first effective Traditional Chinese Medicine approved by the chinese government for the
clinical treatment of both drug-sensitive and -resistant strains of Mycoplasma Pneumoniae (M. pneumoniae). We make
use of two-dimensional gel electrophoresis (2-DE) method to establish the model of Proteomic map on the M. pneumoniae
and explored the mechanisms of the Qinbaiqingfei pellets inhibiting Mycoplasma pneumoniae. Total proteins of 800 μg were
extracted from M. pneumoniae with lysis buffer and was dissected with 2-DE (pI 3–11) and visualized by Coomassie blue
staining. Protein 2-DE maps of the M. pneumoniae showed a good result. Using 2-DE method to establish the model of
Proteomic analysis on the M. pneumoniae laid the groundwork for future studies exploring the mechanisms of this drug.

Biography

Meng Yan-li obtained her Bachelor degree from Harbin medical University and Master degree from Heilongjiang University of the Traditional Chinese Medicine. She
has worked in Heilongjiang Academy of Chinese Medicine Sciences as a research assistant since 2008.

[email protected]

Notes:

J Immunol Tech Infect Dis 2015 Infectious Diseases-2015 Volume 4 Issue 2
ISSN: 2329-9541, JIDIT Hybrid Open Access August 10-12, 2015 Page 166

Sahyun Hong et al., J Immunol Tech Infect Dis 2015, 4:2
http://dx.doi.org/10.4172/2329-9541.C1.003

World Congress on

Infectious Diseases
August 10-12, 2015 London, UK

Isolation of Vibrio cholerae O1 and O139 using immunomagnetic separation

Sahyun Hong, Jong Hyun Kim, Sun Jin Lee, Cheon-Kwon Yoo, Shin-Jung Kang and Gyung Tae Chung
Korea National Institute of Health, Republic of Korea

Background: Detection of pathogenic microorganisms in environmental samples and patient stools is a difficult process.
Objectives: The objective of this study was to develop a method combining immunomagnetic separation (IMS) with PCR or
selection media isolation method for a rapid and easily detection of Vibrio cholerae O1 and O139.
Methods: Magnetic bead were covalently bound with rabbit anti-V. cholerae O1 or O139 via protein G. This research is an
attempt to use magnetic beads for isolating V. cholera O1 and O139 from mixed microorganisms in patient specimen, natural
environment and foods.
Results and Conclusions: Isolation of V. cholerae O1 and O139 from V. cholera NAG, V. parahaemolyticus, V. vulnificus was
examined. In terms of sensitivity and specificity, V. cholerae O1 and O139 1~10CFU/ml couldn’t be isolated but isolation
ratio was higher than 95% at 102CFU/ml. The isolation ratio of V. cholerae O1 and O139 from spiked stools was higher than
90% in average. In particular, the PCR was positive in all samples spending for just one hour in IMS method. Such finding
demonstrated that an IMS method is effective in isolation V. cholerae O1 and O139 from specimen. This method will reduce
laboratory labor, cost, and times.

Biography

Sahyun Hong Completed Ph.D. in the Department of Biotechnology from Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
He is a Staff Scientist in the Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health, Republic of Korea.

[email protected]

Notes:

J Immunol Tech Infect Dis 2015 Infectious Diseases-2015 Volume 4 Issue 2
ISSN: 2329-9541, JIDIT Hybrid Open Access August 10-12, 2015 Page 167

Pereira, A et al., J Immunol Tech Infect Dis 2015, 4:2
http://dx.doi.org/10.4172/2329-9541.C1.003

World Congress on

Infectious Diseases
August 10-12, 2015 London, UK

Use of may-grunwald giemsa stain for microscopic evaluation of cell culture inoculated with microsporidia
in vitro

Pereira, A.1,2, Saraiva, A.M.A1and Lallo, M.A.1,2
1Paulista University
2Centro Universitário São Camilo, Brazil

Microsporidia are obligatory intracellular parasites that infect human and other animals. These pathogens have been
recognized as opportunistic parasites of immunodeficient patients and after the advent of HIV and AIDS, interest
in the in vitro culture of them has been increasingly. In our laboratory we have been used in vitro culture to study the
species Encephalitozoon cuniculi and E. intestinalis in monkey and rabbit kidney cell lines (Vero and RK-13). Recently we
investigated the May-Grunwald Giemsa stain for microscopic evaluation of cells inoculated with E. cuniculi in vitro. Cell lines
were incubated in sterile circular glass coverships in 24-well plates and were inoculated with spores of E. cuniculi. After the
inoculation and incubation, the coverslips were fixed with methanol, stained with May-Grunwald Giemsa, mounted on glass
slides and examined with a light microscope. Each entire coverslip was scanned at a magnification of X 1000. May-Grunwald
Giemsa stain permitted the visualization of parasitophorous vacuoles (PV) containing mature microsporidian spores in the
cytoplasm of the infected cells. The stain used in this study are commonly used in hematology laboratory routine but has not
been used to identify microsporidia spores. This result suggests that May-Grunwald Giemsa is adequate for visualize the PV
containing spores of microsporidia inside the infected cell cultures to study this pathogen in vitro.

[email protected]

Notes:

J Immunol Tech Infect Dis 2015 Infectious Diseases-2015 Volume 4 Issue 2
ISSN: 2329-9541, JIDIT Hybrid Open Access August 10-12, 2015 Page 168