RNI No. DELENG/2004/13061 Published on 28th of previous month PCW/193/2020-2022
Posting Date: 30th of every month Posted at Ex Servicemen Colony PO, Pune-38
Spheroids vs. Organoids
What’s the Difference?
Spheroids and organoids both offer you the opportunity to create more complex three-dimensional models with
the power to more accurately recreate in vivo-like tissue and organ conditions. Each one offers advantages and
disadvantages. Which model works best for you? It largely depends on the type of application you’re working on
and the ultimate goal of your research. In either case, both spheroids and organoids bring incredible promise and
potential to many critical areas of research.
OVERVIEW Spheroids are a simple, Organoids have become an
inexpensive, easy way to model cells in 3D. increasingly popular option for scientists in
The ability of spheroids to replicate solid disease modeling, cancer research, and
tumors helps to accelerate drug discovery drug screening because they resemble
programs and improve our understanding the composition and functionality of organs.
of cancer biology. These can lead to more in vivo-like results.
ORIGIN Spheroids may be generated from Organoids may be generated from
a broad range of cell types resulting pluripotent stem cells and/or organ
in tumor spheroids, embryoid bodies, progenitors from normal or diseased
hepatospheres, neurospheres, and adult tissue-specific stem cells.
mammospheres, among others.
They may be made up of one cell Photo Credit: MDI Laboratory, ME
type or multiple cell types.
Organoids exhibit polarity, cell migration, self-organizing into mini-organs.
Spheroids may or may not exhibit polarity.
POLARITY Proliferating zone
Necrotic core
Quiescent zone
APPLICATION AREA Spheroid Research Areas Organoid Research Areas
• Cancer biology • Organogenesis from stem cells
HOW TO GENERATE • Tumor modeling • Disease modeling including cancer
• Stem cell research • Patient-specific therapies also
• Immuno-oncologyADVANTAGES
• Liver toxicity modeling known as personalized medicine
• CRISPR
Spheroids may be generated and cultured • Immuno-oncology
in suspension or in a low attachment
culture environment such as Photo Credit: Lancaster, MA, et al., 2013
Corning spheroid microplates
or Corning® Elplasia® plates. Organoids may be generated by mixing cells
with an ECM such as Corning Matrigel® matrix
Sample Request for and culturing in media containing specific
Elplasia Plates growth factors to generate mini-organs of
the kidney, thyroid, liver, brain, lung, intestine,
Valid until 31st May prostate, and pancreas.
Spheroids can develop metabolic gradients that create heterogeneous cell Organoids are more complex and in-vivo-like, resembling cell structures and
populations with superior cell-to-cell and cell-to-ECM interactions. They can also microenvironments for more precise and targeted animal research and cell
successfully mimic the microenvironment of a variety of diseased tissue types. therapies. They are used for modeling cancer and organ development. Along
• High reproducibility with CRISPR, they allow for better genetic and drug screening disease models.
• Scalable to different plate formats • Patient-specific
• High throughput screening (HTS) capability • In vivo-like complexity
• Co-culture ability • In vivo-like architecture
• HTS-enabled formats available
India
t 91 124 4604000
f 91 124 4604099
[email protected]
The words you are searching are inside this book. To get more targeted content, please make full-text search by clicking here.
#MAKEINDIACOVID-19FREE
Multiplying efforts to beat COVID-19
The government along with the industry is exploring every other possibility to combat the COVID-19 pandemic whether in the form of vaccines, drugs, diagnostic tests, medical equipment or therapeutic interventions. The major objective is to develop a long term solution by pooling proper resources, sharing knowledge and collaboration.
Discover the best professional documents and content resources in AnyFlip Document Base.
Search