14 MALAYSIAN SYMPOSIUMOF BIOMEDICAL SCIENCE 2024 TH ' Bridging the Gap Breaking Boundaries ’ (14 MYSYMBIOS) 1-2 JUNE 2024 FACULTY OF MEDICINE AND HEALTH SCIENCES, UNIVERSITI PUTRA MALAYSIA (SERDANG CAMPUS) FAKULTI PERUBATAN DAN SAINS KESIHATAN FACULTY OF MEDICINE AND HEALTH SCIENCES Kelab Bioperubatan Jabatan Sains Bioperubatan Fakulti Perubatan dan sains Kesihatan Universiti Putra Malaysia Organised by: Sponsored by: PROGRAMME BOOK TH
TABLE OF CONTENT Participating Universities................................................................................. Message from the Vice Chancellor, Universiti Putra Malaysia (UPM)............. Message from the Dean, Faculty of Medicine and Health Sciences, UPM........ Message from the Head of Department of Biomedical Sciences, UPM.............. Message from the Programme Advisor, 14 MYSYMBIOS............................ About The Program.......................................................................................... Past Symposiums............................................................................................. Organising Committee..................................................................................... Plenary Speakers Professor Ts. Dr. Cheah Yoke Kqueen FASc......................................... Professor Dr. Ahmad Rohi Ghazali........................................................ Forum Speakers Professor ChM. Dr. Nor Hadiani Ismail................................................. Associate Professor Dr. Wan Amir Nizam Wan Ahmad......................... Dr. Mohd Redza Abd Rahman............................................................... Invited Speakers Dr. Rebecca Tay...................................................................................... Mrs. Nur Adani Mohd Hasran................................................................ Mr. Mohd Nazmi Harun......................................................................... Symposium Agenda....................................................................................... Poster Presenters............................................................................................ Abstracts........................................................................................................ Acknowledgement......................................................................................... 2 3 4 5 6 7 8 9 11 12 13 14 15 16 17 18 19 31 38 122 TH 1
PARTICIPATING UNIVERSITIES 2
Assalamu'alaikum warahmatullahi wabarakatuh. It is my distinct honor and privilege to extend a warm welcome to you on the occasion of the 14 Malaysian Symposium of Biomedical Sciences 2024. As we gather to celebrate the remarkable achievements and advancements in biomedical research, I am filled with a profound sense of pride and optimism for the future of our university. This symposium represents a convergence of intellect, innovation, and ingenuity as scholars and researchers from diverse backgrounds come together to exchange ideas, collaborate on projects, and inspire one another towards greater heights of scientific discovery. It serves as a testament to our university's commitment to fostering a culture of excellence and scholarly inquiry in the pursuit of knowledge. At the heart of this symposium lies a shared vision of advancing human health and well-being through cutting-edge research and interdisciplinary collaboration. As we confront the complex challenges of our time, it is imperative that we leverage our collective expertise and resources to address pressing issues in healthcare and biomedicine. I am particularly proud of the contributions made by the Department of Biomedical Science, Faculty of Medicine and Health Sciences along with the students, whose unwavering dedication and commitment to excellence continue to propel us towards new frontiers of discovery. The symposium provides a platform for showcasing their groundbreaking research and fostering collaborations that will shape the future of healthcare delivery in Malaysia and beyond. I extend my sincere gratitude to all participants, organizers, and sponsors for their invaluable support and dedication towards making this event a resounding success. May the discussions and insights gained during the symposium pave the way for transformative breakthroughs that will have a lasting impact on society for generations to come. Warm regards, Dato’ Professor Dr. Ahmad Farhan Mohd Sadullah Vice Chancellor of Universiti Putra Malaysia MESSAGE FROM THE VICE CHANCELLOR Universiti Putra Malaysia 3 th
MESSAGE FROM THE DEAN, FMHS Assalamu'alaikum warahmatullahi wabarakatuh. It brings me immense joy to extend a warm welcome to all attendees and commend the organizing committee at UPM for their efforts in hosting the 14 Malaysian Symposium of Biomedical Science. I acknowledge that this endeavor is entirely student-driven, with the aim of gathering students and researchers from across Malaysia involved in the field of Biomedical Science to share their research findings. Biomedical science stands at the forefront of this endeavor, striving to develop efficient prevention methods, rapid and accurate diagnostics, monitoring tools, and safe therapeutic interventions. This symposium, being both timely and significant, provides a valuable platform to not only reflect on past accomplishments but also to delve into the exploration of future advancements in the field of biomedical science. To the student organizing committee, I extend my heartfelt congratulations once again. Your exemplary teamwork and leadership have been instrumental in ensuring the seamless organization of this vital event. I have full confidence that your hard work will contribute to making this symposium a resounding success. I wish each participant a rewarding and enriching learning experience, and I am optimistic that the collaborative spirit demonstrated here will pave the way for continued advancements in Biomedical Science. Warm regards, Professor Dr. Normala Ibrahim, Dean of Faculty of Medicine and Health Sciences, UPM Universiti Putra Malaysia th 4
It is with great pleasure and anticipation that I extend my warmest greetings to you on the occasion of the 14 Malaysian Symposium of Biomedical Science 2024. As we embark on this exciting journey of scientific exploration and collaboration, I am reminded of the unwavering commitment and dedication that our department has consistently demonstrated towards advancing the frontiers of biomedical research. This symposium serves as an excellent platform for researchers, scholars and practitioners from across the nation to converge, exchange ideas and foster meaningful partnerships. It is a testament to our collective pursuit of excellence and innovation in the field of biomedical sciences. As we navigate the intricate landscape of modern healthcare challenges, it is imperative that we continue to push the boundaries of knowledge and harness the power of interdisciplinary collaboration. The symposium provides us with a unique opportunity to engage in dialogue, share insights, and chart a course towards transformative breakthroughs that will shape the future of healthcare delivery. I am deeply proud of the pioneering work being conducted within our department, and I am confident that the symposium will serve as a catalyst for further advancements in our quest to improve human health and well-being. I extend my heartfelt gratitude to all participants, organizers, and sponsors for their invaluable contributions towards making this event a resounding success. Together, let us embrace the spirit of inquiry, innovation and collaboration as we embark on this enriching journey at the 14 Malaysian Symposium of Biomedical Sciences 2024. Warm regards, Professor Dr. Ling King Hwa, Head of Department of Biomedical Sciences, UPM MESSAGE FROM THE HEAD OF DEPARTMENT Biomedical Sciences, UPM 5 th th
MESSAGE FROM THE PROGRAMME ADVISOR Alhamdulillah. Praise to Allah, for allowing this symposium to be realized. Greetings on behalf of UPM and part of the organizing committee, extending a heartfelt welcome to the 14 Malaysian Symposium of Biomedical Science 2024. This year's thematic essence, "Bridging the Gap and Breaking Boundaries" holds profound significance in propelling scientific advancements and nurturing a fervor for science among the upcoming generation. Biomedical science has consistently been a trailblazer, leading innovations in medical equipment, diagnostic tools, and life-saving treatments. Each passing year sees the field grow in importance and complexity, unveiling new discoveries to address significant challenges faced by society, ranging from global epidemics to chronic illnesses and debilitating conditions. We are privileged to have distinguished experts in biomedical science joining us at this event. Throughout the symposium, they will share their latest research, provide insights into important developments, and discuss the challenges that lie ahead. This symposium serves as a poignant reminder of the indispensable role that biomedical science plays in society, and we eagerly anticipate the discoveries and progress that will emerge from our discussions today. Thank you for being part of this endeavor, and let us collectively explore the exciting possibilities that biomedical science holds for us all. In closing, I extend my sincere wishes for a fulfilling and enlightening experience at the 14 Malaysian Symposium of Biomedical Science 2024. May this symposium be a catalyst for new ideas and discoveries in the ever-evolving field of biomedical science. Warm regards, Dr. Armania Nurdin, Programme Advisor, 14 Malaysian Symposium of Biomedical Science 2024 Biomedical Sciences, UPM th 6 th th
ABOUT THE PROGRAMME The 14 Malaysian Symposium of Biomedical Science 2024, organized by the Biomedical Club UPM in collaboration with the Department of Biomedical Sciences UPM, is dedicated to fostering collaboration, sharing insights, and catalyzing innovation in the field of biomedical science. With its theme "Bridging the Gap & Breaking Boundaries," the symposium encourages young scientists from various universities to present their research, engage with external communities, and overcome challenges by transcending disciplinary boundaries. The legacy of past hosts, including Universiti Tunku Abdul Rahman (UTAR) in 2023, University of Nottingham Malaysia Campus (UNMC) in 2022, International Medical University (IMU) in 2021, Universiti Sains Malaysia (USM) in 2019, the University of Malaya in 2018, and International Islamic University Malaysia in 2017 has significantly contributed to the success and continuity of this prestigious event. This symposium serves as a crucial platform for professional advancement, offering attendees opportunities to refine their skills, broaden their networks, and stay updated on emerging trends. It facilitates the exchange of knowledge and ideas among students and academicians, consolidating comprehensive research through keynote lectures, technical talks, and oral presentations. By propelling translational research forward, the symposium amplifies the influence of biomedical science in enhancing health outcomes and addressing societal challenges. Regardless of their backgrounds—whether aspiring scientists, dedicated researchers, or committed students—the symposium provides an unparalleled opportunity to remain at the forefront of advancements, glean insights from esteemed experts, and actively contribute to unraveling further research secrets in the global biomedical science community. 7 th
PAST SYMPOSIUMS 2021 IMU (ONLINE) 2020 - 2019 USM 2018 UM 2017 IIUM 2016 UPM 2015 UTAR 2014 UKM 2013 USM 2012 UM 2011 IIUM 2010 UPM 8 2022 UNMC (ONLINE) 2023 UTAR 2024 UPM
STUDENT ORGANIZING COMMITTEE PROGRAMME ADVISOR Dr. Armania Nurdin DIRECTOR Nur Amalin Elysha Muhammad Yusri VICE DIRECTOR Arfa Atiqah Zulkeflee Nurul Farah Nasuha Yusne TREASURER Yu Kah Hui Puteri Caymeleia Mohd Shukor PROTOCOL & PROGRAMME Ariesyah Iman Azan Tee Sui Yang Nur Sakinah Jefri Farah Ezizi Mohd Kamuruddin Aisyah Afiqah Abdul Mutalib PUBLICITY Eunice Beh Khoo Jin Ying Vandy Michelle Choong Sook Foon Ang Phei Su LOGISTIC Nur Afrina Esrifairuz Tai Yu Tian Tai Ji Rong Tan Li Ying Neo Yong Qian REGISTRATION Aisyah Iman Ahmad Soalahuddin Al-Thani Nur Hamizah Azziddin Nur Ain Nadia Khairul Zuzaidy Sarah Sa’adah Suhaimi SPONSORSHIP & MARKETING Noor Dini Hannani Azhar Nur Tasnim Amani Zamri Nur Syamimi Muliady Naufa Azzahra Mohd Rashid SECRETARY Mishalini David Ng Qi Wei SCIENTIFIC Shajitra A/P Subramaniam Tiang Ying Ying Siow Bi Xuan Leong Shu Ei PUBLICATION Putri Maisara Khairul Nizat Lye Kemin Emily Ang Uen Yii INVITATION Low Yean Theng Chuah Hui Shan Goh Bin Cheng Sek Jun Cheng FOOD & ACCOMODATION Muhammad Imran Hakim Safiai Nur Rifqah Shamila Mohd Sazali Kamal Hafizi Kamarulizam Aisya Maliha Ummar Jai Kumar DELEGATION Danisha Safia Rustandi Ng Yong Yee Jimmy Loh Zi Qing Rachel Tan Sheen Wei MULTIMEDIA & TECHNICAL Nur Balqis Sabri Ning Ann Gee Kelly Moh Shi Yung Yeong Wei San 9
ADVISORS PROGRAMME ADVISOR Dr. Armania Nurdin PROTOCOL & PROGRAMME Dr. Nur Fariesha Md Hashim Dr. Noraina Muhamad Zakuan Mrs. Hasnijah Alias @ Yakuub Mrs. Nor Aishah Norsabarudin Mrs. Norshazana Nordin REGISTRATION Dr. Seri Narti Edayu Sarchio Dr. Sharifah Sakinah Syed Alwi PUBLICITY Prof. Ts. Dr. Mohd Nasir Mohd Desa Dr. Armania Nurdin INVITATION Dr. Melati Khalid Mrs. Nurul Najwa Manan MULTIMEDIA & TECHNICAL Assoc. Prof. Dr. Tham Chau Ling Assoc. Prof. Dr. Mok Pooi Ling YM Dr. Tengku Azam Shah Tengku Mohamad Dr. Elysha Nur Ismail Mrs. Nurul Syahira Haryani Md Israry Mr. Zulkhairi Zainol DELEGATION Prof. Dr. Muhammad Nazrul Hakim Abdullah Dr. Manraj Singh Cheema SCIENTIFIC Prof. Dato’ Dr. Mohd Roslan Sulaiman Prof. Dr. Latifah Saiful Yazan Assoc. Prof. Dr. Norshariza Nordin Assoc. Prof. Dr. Abdah Md Akim Assoc. Prof. Dr. Roslida Abd Hamid @ Abd Razak Dr. Armania Nurdin Dr. Nurul Akmaryanti Abdullah Dr. Zulkefley Othman PUBLICATION Prof. Ts. Dr. Cheah Yoke Kqueen Dr. Armania Nurdin Dr. Suhaili Abu Bakar @ Jamaludin Dr. Siti Farah Md Tohid Dr. Fezah Othman SPONSORSHIP & MARKETING Dr. Hasni Idayu Saidi Dr. Nurshahira Sulaiman Dr. Hanis Hazeera Harith Dr. Hasiah Ab Hamid FOOD & ACCOMODATION Assoc. Prof. Dr. Thilakavathy Karuppiah Mrs. Nurul Munirah Manan Mrs. Fariza Zainul Abidin Mrs. Hazlen Saleh Mrs. Nora Asyikin Mohd Salim LOGISTIC Dr. Mohd Khairi Hussain Mr. Muhammad Amirul Hakim Malek Mr. Nasrul Ridzal Zainal Abidin Mr. Mohamad Syahlan Shaari Mr. Shamsul Qamar Alias Mr. Muhammad Sabri Mohd Nawawi Mr. Zainal Hassan Mr. Izarul Hakim Rahmad Mr. Mohd Fadzlee Mohd Noh ICT & INFRASTRUCTURE En. Mohd Norazli Che Kob En. Mohammad Norazmie Tarmisan En. Mohd Esham Husain En. Mohd Muazammil Azman En. Muhammad Amir Saad En. Muhammad Qayyum Alemilhadie Sulaiman 10
Prof. Ts. Dr. Cheah Yoke Kqueen is the Deputy Dean, (Graduate Studies, Industry & Community Relations and Income Generation), Faculty of Medicine and Health Sciences, UPM. He is a Fellow of Academy of Science, a registered Medical Laboratory Scientist (Malaysia), a Chartered Scientist, Fellow of Institute of Biomedical Science, UK and Chartered Biologist, Fellow of the Royal Society of Biology, UK and Fellow of the Royal Society of Chemistry, UK. Prof. Dr. Cheah contributes immensely to nongovernmental organisation. He holds the position as the Advisor for Malaysian Biomedical Science Association (MyBiomed), President for BiomedKL and Korean Government Scholarship Alumni. He is the first the Malaysia Allied Health Profession Council for Biomedical. He is also a certified professional in Biorisk Assessment and Biosecurity. Prof. Ts. Dr. Cheah is an established scientist with H-index of 43, more than 280 scientific publications, 5 patents, copyrights and won numerous awards in national and international research exhibitions. He has successfully led 17 grants in the area of Molecular Diagnostics, Genetic Engineering, Drug Discovery, Molecular Biology, Molecular Microbiology, Medical Biotechnology, Molecular Medicine, Genetics, Cancer Biology and Bioinformatics. Prof. Dr. Cheah was awarded as the Top Research Scientist Malaysia in 2017 and was listed in the Malaysia Book of Records in 2022. Professor Ts. Dr. Cheah Yoke Kqueen, FASc Deputy Dean, Graduate Studies, Industry & Community Relations and Income Generation, Faculty of Medicine and Health Sciences, UPM PLENARY SPEAKER BIOGRAPHY 11
PLENARY SPEAKER BIOGRAPHY Professor Dr. Ahmad Rohi Ghazali, is a prominent professor in the Biomedical Science Programme at the School of Diagnostic & Applied Health Sciences, Faculty of Health Science, Universiti Kebangsaan Malaysia (UKM). He specializes in pharmacology and toxicology, where he has made significant contributions to teaching and research. Prof. Dr. Ahmad Rohi coordinates various courses in these fields, integrating MOOCs and blended learning approaches, and serves as the Coordinator of the Health Education Unit and UKM Citra Fellow. Prof. Dr. Ahmad Rohi’s research is focused on cancer chemoprevention, exploring the beneficial effects of local foods, natural products, and their active compounds. He also engages in community research to promote health and occupational safety among Malaysian farmers and students in Tahfiz schools. Prof. Dr. Ahmad Rohi has published around 45 articles in high-impact journals with H-index of 21. He also contributed to a chapter in the third edition of Encyclopedia of Toxicology which was also published. He has received multiple awards for his excellence in teaching and research, including the prestigious Anugerah Bitara Pengajaran UKM in 2021. His academic career is marked by a strong commitment to advancing biomedical sciences and improving health outcomes through education and innovative research. Professor Dr. Ahmad Rohi Ghazali Professor, Faculty of Health Science, Universiti Kebangsaan Malaysia 12
FORUM SPEAKER BIOGRAPHY Professor Dr. Nor Hadiani Ismail obtained her PhD in natural product chemistry from Universiti Putra Malaysia in 1999. She is currently serving as the president of Malaysian Natural Product Society. She was the former director of Atta-ur-Rahman Institute for Natural Products Discovery at the Universiti Teknologi MARA, Malaysia. Recognized as Top Research Scientist Malaysia, she is a fellow of Academy of Science Malaysia and the Malaysian Institute of Chemistry. Professor ChM. Dr. Nor Hadiani Ismail Director of Atta-ur-Rahman Institute for Natural Product Discovery, UITM Puncak Alam Campus, Universiti Teknologi MARA 13
FORUM SPEAKER BIOGRAPHY Dr. Wan Amir Nizam received his medical degree from the Royal College of Surgeons of Ireland (RCSI). He was the acting head of Anaesthesiology at Hospital Kuala Krai, Kelantan, before joining Universiti Sains Malaysia. He earned his PhD in pharmacology, a postdoctoral fellowship with Professor Robert Jones, and a position as a Visiting Scholar at the University of Strathclyde in Glasgow. He is an Associate Professor in Pharmacology, teaching Pharmacology and Biostatistics in School of Health Sciences, USM. He also runs a biostatistics workshop for students and academics, focusing on the GraphPad Prisms program. He is an Associate of the Akademi Sains Malaysia (ASM) and a member of the Advertisement Board of the Ministry of Health, Malaysia. He is currently the President of the Malaysian Society of Pharmacology and Physiology (MSPP), the vice president of the Malaysian Biomedical Science Association (MyBIOMED), and the Malaysian Laboratory Animal Sciences Association (LASAM). He is also a member of the British Pharmacology Society's International Advisory Board Committee (BPS). Associate Professor Dr. Wan Amir Nizam Wan Ahmad Professor, School of Health Sciences, Universiti Sains Malaysia 14
FORUM SPEAKER BIOGRAPHY Dr. Mohd Redza Abd Rahman is a renowned scientist and entrepreneur with a global impact from Malaysia to the United States, Europe, and the United Kingdom. In Malaysia, he collaborates with local icons like Dato' Seri Siti Nurhaliza, Jovian Mandagie, and Marion Caunter to develop consumer health products. Internationally, Dr. Redza is a prominent speaker at European Unionsponsored nutraceutical conferences and a health expert on Malaysian TV channels like TV Al-Hijrah, TV3, and RTM. He also provides health training for major corporations, including PNB, Tabung Haji, MARA, Datum Corp, Petronas, FELDA, PDRM, 4Life Malaysia, Shaklee Malaysia, and Livewave Malaysia. Dr. Redza is a pioneer in nutrigenomics, nutraceuticals, and nutritional health interventions. He serves as the Chief Scientist at a London-based research company specializing in botanical drugs and patented ingredients from cannabis and other plants. His expertise includes ICH guidelines, QA/QC standards, regulatory compliance, isobolographic analysis, pharmacokinetics, and pharmacodynamics. He excels in branding and marketing, positioning nutraceutical and medical cannabis products in competitive markets. As a Key Opinion Leader in Medical Biotechnology, he provides trusted reviews and advice on social media. Dr. Redza's work focuses on using nutraceuticals and nutritional interventions to improve disease management and human health. Dr. Mohd Redza Abd Rahman Scientist Deligateaux (M) Sdn. Bhd. 15
Dr. Rebecca Tay is a distinguished leader in the field of personalized healthcare, with a strong focus on evidence-based advancements and affordable diagnostics. She holds dual PhDs in Pharmacy and Molecular Medicine from the University of Nottingham, UK, and Universiti Putra Malaysia, respectively, and a Master’s degree in Nutrition Physiology from Universiti Putra Malaysia. Dr. Rebecca is the Chief Executive Officer of Beacon Precision Diagnostics (BPD) since 2021, where she leads a team of experts to provide cutting-edge diagnostic services. Under her leadership, BPD has achieved significant milestones, including the development of PRECISE Pharmacogenomic (PGx) and securing exclusive MOUs with industry giants ThermoFisher Scientific and Siemens Healthineers. Her efforts have earned BPD recognition in the Malaysia Book of Records in 2023. In addition to her role at BPD, Dr. Rebecca co-founded the Precision Medicine & Scientific Wellness Association (PMedS), where she serves as Secretary, fostering communication and conscientiousness in the field. She is also the Director of Healthilite Beautilite S/B, focusing on medical nutrition therapy. Dr. Rebecca's academic contributions include serving as a teaching assistant at the University of Nottingham and numerous publications in esteemed journals. She is recognized as an expert speaker and advisor in healthcare, molecular medicine, and wellness on national and international platforms. Her professional certifications include Good Clinical Practice (since 2017), adhering to stringent ethical guidelines in clinical research. Her work embodies integrity, leadership, and a visionary approach to making personalized healthcare accessible to all. INVITED SPEAKER BIOGRAPHY Dr. Rebecca Tay Chief Executive Officer, Beacon Precision Diagnostic 16
Mrs. Nur Adani Binti Mohd Hasran is a dedicated and experienced professional currently serving as the Senior Assistant Director at the Ministry of Investment, Trade, and Industry (MITI) in the Trade and Industry Related Emerging Issues Division / Industry Environmental, Social and Governance (iESG Division). Since April 2021, she has been at the forefront of addressing critical trade and industry-related issues, with a particular focus on integrating environmental, social, and governance (ESG) factors into industry practices. Her role involves formulating policies, conducting indepth analyses, and spearheading initiatives that aim to align Malaysia's trade and industry sectors with global sustainability standards. With over two years and eight months in this role, Mrs. Nur Adani's contributions have been pivotal in advancing MITI's strategic objectives in the evolving landscape of trade and industry. Prior to her current position, Mrs. Nur Adani spent six years as an Assistant Director in the Multilateral Trade Policy and Negotiations Division at MITI, where she played a crucial role in representing Malaysia in international trade negotiations and shaping multilateral trade policies. Her tenure from April 2015 to April 2021 was marked by her adept handling of complex trade issues and her efforts to strengthen Malaysia's trade relations on the global stage. Ms. Nur Adani's journey in public service began as a Kadet Pegawai Tadbir dan Diplomatik at INTAN from March 2014 to March 2015, where she honed her skills in administration and diplomacy. Her extensive experience and unwavering commitment to her work underscore her significant contributions to Malaysia's trade and industry sectors. INVITED SPEAKER BIOGRAPHY Mrs. Nur Adani Mohd Hasran Senior Assistant Director, Trade & Industry Related Emerging Issues Division 17
Mr. Mohd Nazmi Harun is a distinguished professional with a robust academic and research background in biomedical sciences. He earned his Bachelor in Biomedical Science from Universiti Putra Malaysia (UPM) with his thesis focused on the protein profile in Durio zebenthinus seeds. Following his graduation, Mr. Mohd Nazmi served as a Research Assistant and Lecturer Assistant at UPM from June 2005 to December 2006, contributing to cell culture and animal studies in the Department of Human Anatomy. He also gained practical experience as a Pathology Laboratory Technologist (Trainee) at District Hospital Putrajaya in 2004. From January 2007 to January 2015, he worked at Management and Science University (MSU) in Shah Alam, teaching subjects such as Basic Pathology, Histopathology Technique, Cytology, and Basic Microbiology. Additionally, he was involved in developing marketing strategies, organizing events, and reporting on these activities. Mr. Mohd Nazmi has held several key positions in the healthcare sector. From 2015 to 2022, he served as the Head of PR, Marketing & Health Tourism at KPJ Ampang Puteri Specialist Hospital, where he managed international marketing efforts and oversaw the International Patient Centre. Since April 2022, he has been the Head of Marketing & Communication at KPJ Klang Specialist Hospital, where he develops revenue-generating strategies, builds relationships with corporate clients, and manages the hospital’s social media presence. His career reflects a blend of academic excellence and practical application, showcasing his commitment to both educational and professional growth in the biomedical sciences and healthcare marketing. INVITED SPEAKER BIOGRAPHY Mr. Mohd Nazmi Harun Marketing Manager, KPJ Klang Spealist Hospital 18
Time Agenda Venue 08:00 Registration Foyer, FMHS 09:00 Opening Ceremony and Briefing Dewan Kuliah Utama, FMHS 10:00 Morning Tea Poster Viewing Foyer, FMHS 10:30 Presentation Session by Plenary Speaker 1 Professor. Ts. Dr. Cheah Yoke Kqueen FASc “MicroRNA : Advances in Molecular Diagnostics and Therapeutics of Cancer” Dewan Kuliah Utama, FMHS 11:15 Oral and Poster Presentation (Session 1) Oral Dewan Kuliah Utama (Theraupetic and Diagnostic) Dewan Kuliah 3 (Non-communicable diseases) Dewan Kuliah 6 (Others) Poster Foyer, FMHS SYMPOS 19 IUM AGENDA 1 JUNE 2024 ST
Time Concurrent Oral Session 1 11:15 Theraupetic and Diagnostic (Dewan Kuliah Utama) Non-Communicable Diseases (Dewan Kuliah 3) Others (Dewan Kuliah 6) 11:15 OA01: Screening of Limosilactobacillus reuteri 29B (L29B) Culture Media Against Uropathogens Cheong Shi Qin / UPM OB01: Elucidation of Limosilactobacillus reuteri 29B (L29B) Anti-Inflammatory Properties in HCT 116 Cell Line Loi Yong Xin / UPM OC01: In Vitro and In Silico Analysis of Lawsone and Its Analogue (2-Bromo1,4-Naphthoquinone) as Potential Therapeutic Agents in Wound Healing Muhammad Faiz Bin Abdul Samad / UPM 11:30 OA02: Variant Identification Using Whole Exome Sequencing in Family Suspected with IronRefractory Iron Deficiency Anaemia (IRIDA) Nurul Ain Suraya Binti Noor Alif Wira / UPM OB02: Traditional Chinese Medicine (TCM) Body Constitution, Fasting Blood Glucose and Waist Circumference with CAPN10 (rs2975760) and HNF1a (rs735396) Tan Shu Rou / UTAR OC02: Characterization of Periostin 2 (OSF-2) on the Rosetting Phenomenon by Malaria Parasites Phong Zhi Ying / UM SYMPOS 20 IUM AGENDA 1 JUNE 2024 ST
Time Theraupetic and Diagnostic (Dewan Kuliah Utama) Non-Communicable Diseases (Dewan Kuliah 3) Others (Dewan Kuliah 6) 11:45 OA03: The Prevalence of TrimethoprimResistance-Conferring Dihydrofolate Reductase Genes in MultidrugResistant Bacteria From Clinical Isolates in Malaysia Yu Lee Wen / UTAR OB03: A Study to Investigate Glycolysis and Oxidative Phosphorylation Dynamics in HepG2 Cell-Derived 3D Tumourspheres Keoh Li Qing / UM OC03: Characterisation of Staphylococcus aureus from the Nasopharynx of Healthy Children Attending Nurseries in Klang Valley Chew Man Fei / UPM 12:00 OA04: Biomarkers for MicroorganismLinked Airways Inflammation: A Systematic Review Siti Aisyah Binti Zainal Abidin / UPM OB04: Investigating The Intersection: Demographic Profiles and microRNA Expression in Breast Cancer Norlizawati Binti Abdul Hamed / UPM OC04: Investigation of Microbiota Community in the Adult Stages of Aedes aegypti Mosquitoes Voon Zi Xian / UTAR SYMPOS 21 IUM AGENDA 1 JUNE 2024 ST
Time Theraupetic and Diagnostic (Dewan Kuliah Utama) Non-Communicable Diseases (Dewan Kuliah 3) Others (Dewan Kuliah 6) 12:15 OA05: UltrasoundResponsiveness, Cytotoxicity and Mucopenetration Profiling of PolydopamineCoated Ultrasmall Gold Nanoparticle (PUsG) Liew Win Sing / UM OB05: DRP1 Expression Patterns in Benign and Malignant Breast Lesions: A Malaysian Study Leong Wen Kang / IMU OC05: Characterization of Coagulase-Negative Staphylococcus (Cons) from the Nasopharynx of Healthy Children Attending Nurseries in Klang Valley Tony Khoo Cheng How / UPM 12:30 OC06: Production and Characterization of Chitosan from Mucor circineloides Nur Arisya Qamarina Binti Zamri / USM SYMPOS 22 IUM AGENDA 1 JUNE 2024 ST
Time Agenda Venue 13:00 Break Foyer, FMHS Prayer Hall 14:00 Presentation Session by Plenary Speaker 2 Professor Dr. Ahmad Rohi Ghazali “Harnessing Pharmacokinetics of Natural Products for Anti-Cancer Potential” Dewan Kuliah Utama, FMHS 14:45 Oral and Poster Presentation (Session 2) Oral Dewan Kuliah Utama (Theraupetic and Diagnostic) Dewan Kuliah 3 (Non-Communicable Diseases) Dewan Kuliah 6 (Others) Poster Foyer, FMHS SYMPOS 23 IUM AGENDA 1 JUNE 2024 ST
14:45 Concurrent Oral Session 2 Theraupetic and Diagnostic (Dewan Kuliah Utama) Non-Communicable Diseases (Dewan Kuliah 3) Others (Dewan Kuliah 6) 14:45 OA06: Molecular Detection of Capsular Serotypes in Klebsiella pneumoniae Clinical Isolates from Hospital Sultanah Bahiyah (HSB), Kedah Mahelani Binti Mohd Zaini / UPM OB06: Cytotoxic Effect of Malabaricone C Against Human Oral Cancer Cells Nor Hafiza Binti Mohd Rosli / UM OC07: The Sensitisation Profile of Malaysian Atopic Population to Three Major House Dust Mite Allergens and Its Association with Allergic Diseases and Geographic Location Ng Yong Yee / UPM 15:00 OA07: Podoplanin Blockage in Inflammation Toh Yun Xin / UM OB07: The Effect of Lithium Towards Motor Skills and Neurobehavioural Changes in Adult Mice Goh Bing Cheng / UPM OC08: Expression, Purification and Crystallization of Leptospira Protein Noraishah Binti Hairul Nizam / USM SYMPOS 24 IUM AGENDA 1 JUNE 2024 ST
15:15 Theraupetic and Diagnostic (Dewan Kuliah Utama) Non-Communicable Diseases (Dewan Kuliah 3) Others (Dewan Kuliah 6) 15:15 OA08: Proteomic Analysis of Outer Membrane Proteins from CarbapenemResistant Klebsiella pneumoniae Clinical Isolates Nurulain Nabila Binti Abdul Razak / UPM OB08: Development of HepG2-Derived Spheroid Model for Studying Drug Response in Hepatocellular Carcinoma (HCC) Choe Wee Ling / UM OC09: Prevalence of Microbial Species in Air, Surfaces and Skin Among Children in Taska Cahaya Mata, Fakulti Perubatan dan Sains Kesihatan and Taska Cahaya Mata, Hospital Sultan Abdul Aziz Shah Muhammad Firdaus Bin Abd Rahman / UPM 15:30 OA09: Single Nucleotide Polymorphisms in Dengue Virus Isolate from Clinical Samples in Malaysia Chiew Jun Hao / UPM OB09: The Cytotoxic and Anti-Invasive Effects of Auranofin and Luteolin on Colorectal Cancer Cells Chang Joe Yan / UPM OC10: In Silico and Antibacterial Effects of Andricin 01 Against Staphylococcus aureus Jesther Chong See Mun / UM SYMPOS 25 IUM AGENDA 1 JUNE 2024 ST
15:45 Theraupetic and Diagnostic (Dewan Kuliah Utama) Non-Communicable Diseases (Dewan Kuliah 3) Others (Dewan Kuliah 6) 15:45 OA10: Serotype Distribution of Dengue Virus in Samples Collected from Two Hospitals: Hospital Tuanku Ja’afar Seremban and Hospital Cyberjaya Nur Aida Binti Mohamad Shah / UPM OB10: Cytotoxic Effect of Malabaricone B Against Human Oral Cancer Cells Dhamirah Irdina Binti Mohammad Azzuddin / UM OC11: Exploring the Modulatory Effects of Lawsone and Its Derivative Menadione on Cell Migration in Wound Healing: An In Vitro and In Silico Studies Hariz Syahmi Bin Hazandy / UPM 16:00 OB11: The AntiInvasive Effects of 1- Methylpropyl 2- Imidazolyl Disulfide (Px-12) on HypoxiaInduced Colorectal Cancer Cells and Its Underlying Mechanism of Action Chong Qiao Yi / UPM OC12: Revitalizing Pulp: BMP-7's Influence on Wjmsc and DHDP through Histology and Viability Nur Athirah Binti Ahmad Shuhaimi / UKM Time Agenda Venue 17:00 Evening Tea Foyer, FMHS End Of Day 1 SYMPOS 26 IUM AGENDA 1 JUNE 2024 ST
SYMPOSIUM AGENDA 2 JUNE 2024 Time Agenda Venue 08:00 Registration and Breakfast Foyer, FMHS 09:00 Forum Session 1 “ From Forest to Industry: Unlocking the Potential of Natural Products” Speakers: Professor ChM. Dr. Nor Hadiani Ismail Associate Professor Dr. Wan Amir Nizam Wan Ahmad Dr. Mohd Redza Abd Rahman Moderator: Nisa Hadirah Norazar Dewan Kuliah Utama, FMHS 10:00 Oral and Poster Presentation (Session 3) Oral Dewan Kuliah Utama (Natural Products) Poster Foyer, FMHS 10:00 Concurrent Oral Session 3 Natural Products (Dewan Kuliah Utama) 10:00 OD01: Antimicrobial Activity of Malabaricone C Against Oral Bacteria Nur Fathihah Binti Nordin / UM 27 ND
10:15 Concurrent Oral Session 3 Natural Products (Dewan Kuliah Utama) 10:15 OD02: The Antimalarial Activity of the Peel Extract of Garcinia mangostana and the Compounds, Goniothalamin from Goniothalamus tapis and Acetoxychavicol Acetate from Alpinia conchigera Against the Malaria Parasite, Plasmodium falciparum Ahmad Firdaus Bin Ahmad Sukri / USM 10:30 OD03: Wound Healing Properties of Green Synthesized Zinc Oxide Nanoparticle, Copper Oxide Nanoparticle, and Zinc/Copper Nano Composite from Citrus sinensis Peel Extract on Human Skin Cells Chin Jia Hong / UTAR 10:45 OD04: The Effects of Plukenetia volubilis Against Memory Deficits in Lipopolysaccharide-Induced Female Rat Model of Alzheimer’s Disease Lee Choon Yung / USM 11:00 OD05: Preliminary Investigation on the Immunomodulatory Mechanism of Sang Huang (Phellinus linteus LiGNO-SH02) Extract (xSH ) on Macrophages Phoebe Tee Yon Ern / TAYLOR’S UNIVERSITY 11:15 OD06: Bactericidal Action of Stingless Bee Honey Against Pathogenic Escherichia coli Kang Chiao Sim / UTAR 28 TM SYMPOSIUM AGENDA 2 JUNE 2024 ND
Time Agenda Venue 11:30 Morning Tea Poster Viewing Foyer, FMHS 11:45 Oral and Poster Presentation (Session 4) Oral Dewan Kuliah Utama (Natural products) Poster Foyer, FMHS 11:45 Concurrent Oral Session 4 Natural Products (Dewan Kuliah Utama) 11:45 OD07: Evaluation of Antioxidant Properties and Chemical Composition of Spirulina and Chorella Extracts for Face Cream Formulation Dhanaluxmi Pillay Ramasawmy / TAYLOR'S UNIVERSITY 12:00 OD08: PhytochemicalAnalysis and Antibacterial Activity of Myristica fragrans Fruit Extracts Against Human Pathogenic Bacteria Darren Yong Seow Tien / UTAR 12:15 OD09: The Effects of Plukenetia volubilis on Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome in Sprague Dawley Rats Muhammad Syarif Bin Zainal / USM 12:30 OD10: Biochemical Composition and Antioxidant Properties of Chlorella Strain from Polar and Tropical Regions Chloe Wong Zi En / TAYLOR’S UNIVERSITY SYMPOS 29 IUM AGENDA 2 JUNE 2024 ND
Time Agenda Venue 13:00 Break Foyer, FMHS Prayer Hall 14:00 Forum Session 2 “ Alumni Career Talk” Speakers: Dr. Rebecca Tay Mrs. Nur Adani Mohd Hasran Mr. Mohd Nazmi Harun Moderator: Nur Adeilah Norman Dzulkarnain Dewan Kuliah Utama, FMHS 15:15 Closing Ceremony Dewan Kuliah Utama, FMHS 17:15 Evening Tea Foyer, FMHS End Of Day 2 SYMPOS 30 IUM AGENDA 2 JUNE 2024 ND
POSTER PRESENTERS 1 JUNE 2024 Research Category Presenter’s ID Name / University Research Title Theraupetic and Diagnostic PA01 Adeline Tiaw Yuen Shan / UTAR Screening for Antibiofilm Effect of Peptide PAM-6 on Pseudomonas aeruginosa (ATCC27853) Theraupetic and Diagnostic PA02 Nafiessa Alya Binti Mohd Najib / UPM Evaluation of Cytotoxicity of Carbon Monoxide-Releasing Molecule 2 (CORM-2) on A549 Lung Epithelial Cells Theraupetic and Diagnostic PA03 Cheng Jia Ai / UTAR Study of Mechanisms of Actions of Antibacterial Peptide PAM-6 on Escherichia coli and Pseudomonas aeruginosa Theraupetic and Diagnostic PA04 Chong Zi Jian / UM Investigating the Activity of CRISPR/Cas9 Ribonucleoprotein Complex (RNP) Targeting CD73 for Cancer Immunotherapy Theraupetic and Diagnostic PA05 Muhammad Fitri Hakimi Bin Zulkifli / UPM Molecular Detection of Microsporidia spp. Among Rohingya School Children in Selangor (Session 1) TIME: 11.15 am - 1.00 pm 31 ST
Research Category Presenter’s ID Name / University Research Title Noncommunicable diseases PB01 Nur Izzah Binti Muhammad Kamil / UPM Exploring the Effects of Zerumbone on the Expression of InvadopodiaRelated Proteins in Colon Cancer Cells: A Molecular Investigation Noncommunicable diseases PB02 Yong Qiao Ru / IMU Melatonin Regulates Pyroptosis by Targeting the Caspase-1/Caspase-4/ Caspase-5/GSDMD/ HMGB1 Pathway in Hep G2 Cell Noncommunicable diseases PB03 Leong Khei Min / UPM Anticancer Properties of Limosilactobacillus reuteri 29B Strain in HeLa Cell Line Noncommunicable diseases PB04 Ashlyn Yau Wen Ning / TAYLOR’S UNIVERSITY Unlocking Therapeutic Insights: Cytotoxic Evaluation of Malaysian Agarwood Leaf Extracts on Melanoma Cells Noncommunicable diseases PB05 Kelly Chin Yu Han / USM The Cytotoxic Effects of Malaysian Tualang Rock Honey and Trigona Honey on MDA-MB-231 Breast Cancer Cell Line (Session 1) POSTER PRESENTERS 32 1 JUNE 2024 TIME: 11.15 am - 1.00 pm ST
Research Category Presenter’s ID Name / University Research Title Others PC01 Nur Tha’iyyah Binti Kamaruzaman / UPM Effects of HCoV-229E on Human Platelet Aggregation Induced by Epinephrine Others PC02 Chew Wing Xin / TAYLOR'S UNIVERSITY Biocomputational-Mediated Screening for Discovery of Antimelanogenic and Antioxidant Agents from Ficus carica L. Others PC03 Farah Syazwanie Binti Mohd Zahiruddin / UPM Effects of HCoV-229E on Human Platelet Aggregation Induced by Collagen Others PC04 Chin Joo Yie / UM Effect of Cytoadherence Properties of Plasmodium knowlesi Others PC05 Wan Muhammad Daud Bin Wan Ali / UPM Prevalence of Intestinal Polyparasitism Coinfected with Microsporidia Among Rohingya School Children in Selangor (Session 1) POSTER PRESENTERS 33 1 JUNE 2024 TIME: 11.15 am - 1.00 pm ST
Research Category Presenter’s ID Name / University Research Title Theraupetic and Diagnostic PA06 Zuliana Anisah Binti Zulkifli / USM Suitability of HighResolution Melt (HRM) Analysis for Single Nucleotide Polymorphism Detection in the Pattani Malay Population Theraupetic and Diagnostic PA07 Lee Huei Yiun / UM The Study of Nudt15 Genotypes in Malaysian Patients with Crohn’s Disease Theraupetic and Diagnostic PA08 Tham Xuan Yao / UPM Characterization of Human Microglial Model Involved in Methamphetamine-Induced Neuroinflammation Theraupetic and Diagnostic PA09 Amar Harris Bin Arifin / USM Immunohistochemistry (IHC) Expression of Endothelial Cell-Specific Molecule-1 (ESM-1) as Predictive Biomarkers in TripleNegative Breast Cancer (TNBC) Patients Noncommunicable diseases PB06 Maizatul Harnani Binti Mazlan / UPM Anti-Obesity Effect of Daidzein on Metabolic Syndrome Sprague-Dawley Rats Accompanied with Benign Prostatic Hyperplasia Noncommunicable diseases PB07 Thiiben A/L Krishnan Sami / TAYLOR’S UNIVERSITY The Therapeutic Potential of Inonotus obliquus, Antrodia camphorata, and Phellinus linteus Mushroom Extracts on Breast Cancer Cells Noncommunicable diseases PB08 Nur Sorfeena Sufi Binti Mara Ridhuan / UPM The Effects of Eczefolia on the Migration of HCT116 Colon Cancer Cells (Session 2) POSTER PRESENTERS 34 1 JUNE 2024 TIME: 2.45 pm - 5.00 pm ST
(Session 2) Research Category Presenter’s ID Name / University Research Title Others PC06 Goh Chia Yee / UTAR Screening for Antibacterial and Antibiofilm Effect of Synthetic Peptide PAM-5 on Multidrug-Resistant Escherichia coli Others PC07 Nadiah Nabilah Binti Mohd Marphy / UPM Effects of Human Coronavirus (HCoV-229E) on Human Platelet Aggregation Induced by ADP Others PC08 Sherly Lam Wai Ying / UM Establishment of Gametcoytes Production in Plasmodium knowlesi In Vitro Culture Others PC09 Nur Amisya Safia Binti Mohd Fadzil / UPM Candida glabrata Gene Expression Response Upon Engulfment by Macrophages Others PC10 Ahnaf Akram Bin Mohamad Khairul Anuar / UM Insecticide Susceptibility Profile of Wolbachia-Infected Aedes aegypti POSTER PRESENTERS 35 1 JUNE 2024 TIME: 2.45 pm - 5.00 pm ST
(Session 3) Research Category Presenter’s ID Name / University Research Title Natural Products PD01 Ng Hui Yee / UPM The Antiviral Effects of Different Types of Stingless Bee Propolis Extracts Against Influenza A Virus Natural Products PD02 Mok Xin Hui / UM Comparative Analysis of Resveratrol from Various Sources in Inhibiting Dengue Virus Replication Natural Products PD03 Narmitha A/P Sathasivam / UPM The Effect of Polymeric Micelles Encapsulating Sesbania grandiflora Leaf Extract on the Antibacterial Activity of Gram-Positive and Gram-Negative Bacteria Natural Products PD04 Nur Dyana Syazwanie Binti Dedy Chandra / USM Optimization in Extraction and Characterization of Chitosan from Aspergillus niger Natural Products PD05 Teoh Chai Wen / UTAR Antioxidant Activity and Cytotoxic Effect of Traditional Chinese Medicine (TCM) Decoction Natural Products PD06 Sitinurshahfika Binti Abdullah / UPM Evaluating the Toxicity of Nanoencapsulated Sesbania grandiflora Ethanolic Extract on Zebrafish Embryos Natural Products PD07 Muizzuddin Bin Jamaluddin / USM The Antioxidant Properties and Toxicity Evaluation of Ostodes pauciflora Merr. Seed Extract (Kop Nuts) POSTER PRESENTERS 36 2 JUNE 2024 TIME: 10.00 am - 11.30 am ND
(Session 4) Research Category Presenter’s ID Name / University Research Title Natural Products PD08 Intan Nurzulaikha Binti Abdul Zahid / UPM Nanoencapsulation of Moringa oleifera Leaf Extract (nanoMOLE) Using Thin Dry Film Method: Synthesis, Characterisation and Toxicity Assessment Using Zebrafish (Danio rerio) Model Natural Products PD09 Tan Jing Ming / UTAR Antioxidant Activity and Cytotoxic Effect of Lemongrass Essential Oil Natural Products PD10 Nur Liyana Binti Daud / UPM Nanoencapsulation of Moringa oleifera Leaf Extract (nanoMOLE) Using Thin Dry Film Method: Synthesis, Characterization and Assessment of Antibacterial Activities Against Methicillin-Resistant Staphylococcus aureus (MRSA) Natural Products PD11 Lam Min Jun / UTAR Impacts of Stingless Bee Honey on the Morphology Intactness of Escherichia coli Natural Products PD12 Evon Koay Yi Wen / USM Effects of Melon Manis Terengganu Peel Powder on Cell Proliferation and Morphological Changes in Human Foetal Osteoblast Cell Line (hFOB 1.19) Natural Products PD13 Phang Chun Zhong / UTAR Larvicidal Bioefficacy of the Methanolic Extracts from Fruit Wastes of Citrullus lanatus Rinds and Lansium domesticum Peels Against Aedes spp. Larvae POSTER PRESENTERS 37 2 JUNE 2024 TIME: 11.45 am - 1.00 pm ND
38 ' Bridging the Gap & Breaking Boundaries’
Presenter’s ID: OA01 Category: Therapeutic and Diagnostic SCREENING OF Limosilactobacillus reuteri 29B (L29B) CULTURE MEDIA AGAINST UROPATHOGENS Cheong Shi Qin , Ang Li Hui ,Melati Binti Khalid , Leslie Than Thian Lung Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. *Corresponding author email address: [email protected] Uropathogenic infections represent a significant global public health concern, affecting individuals of all ages and genders. Lactobacillus, particularly Limosilactobacillus reuteri, has emerged as a promising probiotic strain for mitigating such infections. Previous research has highlighted the potent antimicrobial properties of the supernatant from lactic acid bacteria, which are attributed to the organic compounds they produce. This study aimed to screen and assess the inhibitory effects of L29B postbiotics against uropathogens and determine the minimum inhibitory concentration (MIC) of the L29B CFS. Different culture media formulations were used to culture an isolated vaginal Lactobacillus strain, Limosilactobacillus reuteri 29B (L29B), and the resulting cell-free supernatant (CFS) was collected for further investigation. A microtiter plate assay was used to evaluate the antimicrobial activity of culture media. Three culture media that exhibit excellent inhibitory effects were further treated with proteinase K, catalase, heat and pH neutralization to determine the characteristics of the postbiotics. Lastly, broth microdilution methods were used to determine the MIC of L29B culture media against uropathogens. For the culture media screening, F12, F15 and F18 showed good inhibitory effects against E.coli ATCC53498 and E.faecalis ATCC51299; whereas the F2, F16, and N5 formulations showed good inhibitory effects against C.albicans ATCC14053. In the L29B characterization, catalase and proteinase K treatments did not affect the potency of L29B CFS against all three uropathogens. However, in the first screening, heat-treated CFS showed an increased inhibitory effect against C.albicans but not against E.coli and E.faecalis. Different formulations of the L29B culture medium showed different inhibitory effects against uropathogens. The inhibitory effects of L.reuteri may be attributed to the production of organic acids and metabolic waste compounds. These results suggest that L.reuteri CFS is a potential postbiotic that can mitigate uropathogenic infections. Keywords: Limosilactobacillus reuteri, antimicrobial, uropathogens 39 1* 2 1 2 1 2
Presenter’s ID: OA02 Category: Therapeutic and Diagnostic VARIANT IDENTIFICATION USING WHOLE EXOME SEQUENCING IN A FAMILY SUSPECTED WITH IRON-REFRACTORY IRON DEFICIENCY ANAEMIA Nurul Ain Suraya Noor Alif Wira , Asral Wirda Ahmad Asnawi , Noor Haliza Mohamed Ibrahim , Nurul Huda Musa , Shankar Aissvarya , Mandy Yap Yee Yee , Jameela Sathar , Veena Selvaratnam , Karuppiah Thilakavathy Department of Biomedical Science, Faculty of Medicine, Universiti Putra Malaysia, Serdang Selangor, Malaysia. Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Nilai, Negeri Sembilan, Malaysia. Makmal Rujukan Klinikal Hematologi, Department of Hematology, Hospital Ampang, Selangor, Malaysia. *Corresponding authors' email: [email protected], [email protected] Iron-refractory iron deficiency anaemia (IRIDA) is a rare type of anaemia that affects about 1 in 1 million people globally. The inheritance pattern is autosomal recessive, which is caused by a defective Transmembrane Protease Serine 6 (TMPRSS6) gene. Patients with TMPRSS6 variants exhibit microcytic hypochromicity, low serum iron and transferrin saturation (TSAT), relatively low serum ferritin, normal to high hepcidin, and unresponsive to oral iron. Based on our knowledge, no genetic studies have been conducted on IRIDA in Malaysia. This study aims to describe the molecular genetic findings in a family suspected of IRIDA using whole exome sequencing (WES). Two male siblings with persistent and unexplained anaemia and three male family members who were not having symptoms were recruited for this study at the Anaemia Clinic, Hospital Ampang. DNA was extracted from blood samples and sent for WES service. Bioinformatic analysis was performed to identify the presence of gene variants related to IRIDA. Gennext software (Version: 0.1.0) was utilised for variant annotation, and various databases were used for the variant filtering and prioritisation process such as NCBI, OMIM, HPO, etc. Haematological parameters revealed that the two suspected sons had microcytic hypochromic anaemia, low haemoglobin and TSAT, and normal serum hepcidin. The proband had low serum iron and normal ferritin, while the other suspected son had relatively low serum iron and ferritin. All the other three male family members showed normal haematological parameters. Pathogenic variants were not found in the family members; however, common SNPs related to IRIDA were identified. All five family members carried homozygous TMPRSS6/rs855791 (NM_001289000.1:c.2246T>C) and SLC11A2/rs445520 (NM_001174130.2:c.17A>C). The two suspected siblings carried heterozygous TMPRSS6/rs2235324 (NM_001374504.1:c.730A>G) but not the TMPRSS6/rs78174698 (NM_001374504.1:c.1636C>T) that was found in the father and first son. In addition, the proband was found to carry a homozygous splice-site deletion variant, TMPRSS6/rs60484081 (NM_001374504.1:c.1842-6_1842-2delCTGGGG). The haematological parameters of the suspected siblings point towards the IRIDA phenotype. rs78174698 was reported to increase serum ferritin, whereas the absence of this variant probably reduces serum ferritin in the suspected siblings, an indication of IRIDA. rs60484081 could be a modifier SNP that causes reduced penetrance in the proband, showing less severe symptoms compared to his brother. These findings emphasised that WES can be used in diagnosing underlying IRIDA that affect this family. Thus, advancements in diagnostic tools help families in need get better treatment for IRIDA. Keywords: IRIDA, TMPRSS6, SNP 40 1 1 2,3* 1 1 3 3 3 1* 1 2 3
Presenter’s ID: OA03 Category: Therapeutic and Diagnostic THE PREVALENCE OF TRIMETHOPRIM-RESISTANCE-CONFERRING DIHYDROFOLATE REDUCTASE GENES IN MULTIDRUG-RESISTANT BACTERIA FROM CLINICAL ISOLATES IN MALAYSIA Lee Wen Yu, Choy Hoong Chew Department of Allied Health Sciences, Universiti Tunku Abdul Rahman (UTAR), Kampar, Perak. *Corresponding author email address: [email protected], [email protected] Antibiotic resistance has been a global issue since the appearance of drug-resistant microorganisms. Trimethoprim, which is commonly used to treat uncomplicated urinary tract infections in combination with sulfamethoxazole, is losing effectiveness due to increased resistance rates. Gram-negative bacteria typically develop trimethoprimsulfamethoxazole resistance by gaining the dfr and sul genes, frequently found on the gene cassette located on the integron. The purpose of this study was to investigate the prevalence of dfrA genes in 60 clinical isolates, and their antimicrobial susceptibility to seven antibiotics from five classes: β-lactam combination agents, folate pathway antagonists, quinolones, aminoglycosides, and polymyxins. Triplex polymerase chain reaction (PCR) was performed to detect the dfrA genes in total DNA extracted using the fast-boil method. The susceptibility of the isolates was determined using the Kirby-Bauer disc diffusion method. Antimicrobial susceptibility data revealed that 76.67% of the bacterial isolates were resistant to amoxicillin-clavulanic acid, 70.00% to trimethoprimsulfamethoxazole, 68.33% to ciprofloxacin, 61.67% to nalidixic acid, 18.33% to tobramycin, 8.33% to netillin, and 5.00% to polymyxin B. In triplex PCR, eight isolates were positive for dfrA1 (13.33%), nine for dfrA7 (15.00%), and none for dfrA17. Cocarriage of the dfrA genes was not seen. The Chi-square test or Fisher's exact test was used to evaluate the association between dfrA genes and antimicrobial resistance phenotypes, as well as the patient's age and gender. The findings indicated that dfrA7 was positively associated with ciprofloxacin and trimethoprim-sulfamethoxazole resistance. Other dfrA gene variants, along with integron-carried dfr and sul genes, may cause trimethoprimsulfamethoxazole resistance. Additionally, plasmid-mediated quinolone resistance genes on plasmids with integron-carried dfrA genes can confer resistance to both fluoroquinolones and trimethoprim-sulfamethoxazole. Patient’s age and gender showed no significant association with dfrA gene prevalence, but the dfrA-positive isolates were more common in the working-age and old age groups, suggesting increased multidrugresistant infection risk from hospital visits. Keywords: Antibiotic Resistance, Trimethoprim-sulfamethoxazole, dfrA genes 41
Presenter’s ID: OA04 Category: Therapeutic and Diagnostic BIOMARKERS FOR MICROORGANISM-LINKED AIRWAYS INFLAMMATION: A SYSTEMATIC REVIEW Siti Aisyah binti Zainal Abidin , Juliana binti Jalaludin , Suhaili binti Abu Bakar @ Jamaludin Department of Biomedical Science, Faculty of Medicine and Health Science, University Putra Malaysia, Serdang, Selangor, Malaysia. Department of Environmental and Occupational Health, University Putra Malaysia, Serdang, Selangor, Malaysia. *Corresponding author email address: [email protected] Microorganism-linked airways inflammation is a complex condition with significant clinical implications. Identifying reliable biomarkers in the airways inflammation can improve diagnostic accuracy and guide treatment strategies. Thus, this systematic review is to investigate biomarkers associated with the airways inflammation induced by microorganism exposure. The review was conducted following the Preferred Reporting for Systematic Reviews (PRISMA) guidelines. Search strategies were applied to multiple databases, including PubMed, Scopus, and EBSCOhost. The study employed a comprehensive search strategy utilizing relevant keywords such as "Airways," "Biomarkers," "Biomolecular markers," "Biological indicators," "Airways inflammation," and "Respiratory inflammation." Eligible studies were included based on predefined criteria: English language, full-text articles reporting on biomarkers and airways inflammation published from 2019 to 2024. Selected articles underwent full-text assessment after title and abstract screening to ensure alignment with the research question. Data extraction included study year, first authors’ surname, participants’ demographics, biomarkers investigated, study design, interventions, and outcome measures. Downs and Black Checklist has been used to ensure a comprehensive assessment of reporting quality, external and internal validity, bias, confounding, and primary outcome identification in this review. This review identifies a spectrum of biomarkers linked to microorganism-induced airways inflammation, encompassing FeNO, Interferon-gamma (IFN-γ), C-Reactive Protein (CRP), and numerous others. Moreover, they can be classified into several categories of biomarkers, such as immunological and biochemical biomarkers, and these biomarkers possess the potential to enhance diagnostic precision, prognostic evaluation, and therapeutic effectiveness in respiratory conditions influenced by microbial colonization. Biomarkers play a crucial role in the diagnosis and management of microorganism-linked airways inflammation. Further research is needed to validate these findings and establish standardized protocols for biomarker use in clinical practice. Keywords: Airways inflammation, Systematic review, C-Reactive Protein (CRP) 42 1 2 1* 2 1
Presenter’s ID: OA05 Category: Therapeutic and Diagnostic ULTRASOUND-RESPONSIVENESS, CYTOTOXICITY AND MUCOPENETRATION PROFILING OF POLYDOPAMINE-COATED ULTRASMALL GOLD NANOPARTICLE (PUsG) Win Sing Liew , Yin Yin Teo , Dennis W. Hwang , Lik Voon Kiew Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia. Department of Chemistry, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia. Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan. Department of Pharmacology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia. Department of Biological Science and Technology, College of Biological Science and Technology, National Yang-Ming Chiao Tung University, Hsinchu 30010, Taiwan. *Corresponding author email address: [email protected] The tumour delivery of anticancer drugs in the intravesical therapy (IVT) of bladder cancer is inefficient due to poor penetration of drugs through the mucous layer that lines the inner surface of the bladder. This leads to incomplete tumour annihilation and increased risk of cancer recurrence. Here we report the development of a new polydopamine-coated ultrasmall gold nanoparticles (PUsG) that (i) effectively penetrate a simulated mucous layer and (ii) produce cytotoxic free radicals upon ultrasound irradiation. We hypothesize that the PUsG may overcome the bladder drug penetration barrier and provide continuous supply of cytotoxic free radicals to destroy the cancer tissue when the bladder cancer region is focally irradiated with focus ultrasound. PUsG were synthesized by coating PDA onto ultrasmall gold nanoparticles (UsG) at an optimised PDA:UsG ratio. The average size of PUsG was 23.5 ± 3.5 nm with polydispersity index (PDI) of 0.60 ± 0.05 and zeta potential of -48.6 ± 5.7 mV. The successful synthesis of PUsG were confirmed by (i) the shift of characteristic UsG peak at around 526 nm in UV-Vis spectra, (ii) overall increase in size and change in zeta potential after PDA coating (DLS) and (iii) the presence of functional group vibrations (indicating the presence of PDA on the PUsG) in FTIR spectra. The performance of PUsG was then evaluated based on free radical generation upon ultrasound irradiation, mucopenetration and cytotoxicity as compared to UsG. PUsG had exhibited a 12% higher rate of ROS generation compared to UsG, suggesting enhanced efficiency of PUsG in generating cytotoxic ROS. Furthermore, PUsG was found to show similar mucopenetration rate to UsG in vitro (simulated mucous Transwell model), indicating the need for further improvement. The overall results suggest the potential of PUsG as an ultrasound-activated ROS-generating agent for bladder cancer treatment (via SDT), and support further optimization of PUsG for better mucopenetration. Keywords: Gold nanoparticles, Polydopamine (PDA), Sonodynamic therapy (SDT) 43 1 2 3 4 5 1 2 3 4,5*
Presenter’s ID: OA06 Category: Therapeutic and Diagnostic MOLECULAR DETECTION OF CAPSULAR SEROTYPES IN Klebsiella pneumoniae CLINICAL ISOLATES FROM HOSPITAL SULTANAH BAHIYAH (HSB), KEDAH Mahelani Mohd Zaini , Hazmin Hazman , Siti Norbaya Masri ,Mohd Nasir Mohd Desa , Nurshahira Sulaiman Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia. *Corresponding author email address: [email protected] Klebsiella pneumoniae is a gram-negative bacteria known to colonize the human mucosal membranes. It is associated with community-acquired and nosocomial infections and causes a wide range of diseases, including pneumonia and liver abscess. In recent years, the emergence of carbapenem-resistant K. pneumoniae (CRKP) has increased worldwide. Infections caused by CRKP constitute a major threat to public health and are strongly associated with high rates of mortality, especially in immunocompromised and critically ill patients. This study aims to investigate the associations between K1, K2, K5, and K54 capsular serotypes with hypermucoviscosity and carbapenem resistance in K. pneumoniae isolates. A total of 196 K. pneumoniae isolates were collected from Hospital Sultanah Bahiyah (HSB), Kedah, Malaysia. Isolates were re-identified using a series of biochemical tests. Hypermucoviscosity test was performed on the isolates to detect isolates with a hypermucoviscous phenotype. Subsequently, multiplex PCR was carried out to classify the isolates into K1, K2, K5, and K54 serotypes, respectively. Nineteen out of 196 isolates (9.7%) were detected as CRKP by antimicrobial susceptibility testing. Capsular serotype K2 was the most prevalent [n = 38; 19.4%], followed by K1 [n = 22; 11.2%], K5 [n = 14; 7.1%], and K54 [n = 4; 2%]. On the other hand, 60% of the isolates (118/196) were untypable. Statistical analysis revealed that only the K1 and K2 serotypes showed a significant association with hypermucoviscosity (p = 0.003 and p = 0.049, respectively). However, the study showed that CRKP isolates were not associated with K1, K2, K5, and K54 serotypes. The study found that K1 and K2 serotypes were associated with the hypervirulent strains of K. pneumoniae. However, none of the serotypes were associated with the CRKP. This study revealed a concerning prevalence of CRKP (9.7%). This warrants comprehensive investigations focusing on the common virulence-associated genes to understand the molecular characteristics and mechanisms underlying CRKP. Keywords: Klebsiella pneumoniae, hypermucoviscosity, carbapenem resistance 1 2 1* 1 1 2 1 44
Presenter’s ID: OA07 Category: Therapeutic and Diagnostic PODOPLANIN BLOCKADE IN INFLAMMATION Yun Xin Toh , Won Fen Wong Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia. Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia. *Corresponding author email address: [email protected] Multiple sclerosis (MS) and neuromyelitis optica spectrum disorders (NMOSD) are autoimmune diseases characterized by neuroinflammatory damage modulated by immune cells including microglia/macrophages. Podoplanin (PDPN), a transmembrane protein, has been associated with inflammation caused by infections or autoimmune diseases. PDPN is upregulated particularly in the inflammatory macrophages and functions to control cellular phagocytosis, polarisation, and mobility. Thus, we hypothesized that targeting PDPN may be a potential approach in reducing inflammation. This study aims to (i) examine the inflammatory status of autoimmune demyelinating disease (MS and NMOSD) patients and (ii) investigate the effects of antibody-mediated PDPN blockade in inflamed THP-1 cells. First, the inflammatory status of MS and NMOSD patients at active or remission phase was measured by ELISA to quantitate their serum soluble PDPN (sPDPN) level. Next, THP-1 cells were infected with Helicobacter pylori (H. pylori) at MOI 10 to induce inflammation and subsequently treated with anti-human PDPN antibody (clone NC-08), followed by the measurement of proinflammatory cytokines (IL-1β, IL-6 and TNF-α) concentration. Interestingly, findings showed a significantly higher serum sPDPN concentration in MS patients who were at active disease phase but not at remission phase. For NMOSD patients, no significant differences can be found between the patients at the active or remission phases. Meanwhile, the H. pylori-infected THP-1 cells exhibited a 3-fold increase of cell surface PDPN in flow cytometrical analysis, but PDPN expression was not downregulated with the increasing anti-PDPN treatment. Unexpectedly, the anti-PDPN antibody increased the proinflammatory cytokines in a dose-dependent manner, suggesting that clone NC-08 antibody used in this study acts as an agonist, rather than antagonist, in promoting the secretion of proinflammatory cytokines. In conclusion, the PDPN blockade using clone NC-08 antibody did not limit the inflammation, but further study can be done using different clones of blocking antibody. Keywords: Multiple sclerosis, inflammation, podoplanin 1 2* 1 2 45
Presenter’s ID: OA08 Category: Therapeutic and Diagnostic PROTEOMIC ANALYSIS OF OUTER MEMBRANE PROTEINS FROM CARBAPENEM-RESISTANT Klebsiella pneumoniae CLINICAL ISOLATES Nurulain Nabila Abdul Razak, Hazmin Hazman, Mohd Nasir Mohd Desa, Nurshahira Sulaiman Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia. *Corresponding author email address: [email protected] Carbapenem resistant Klebsiella pneumoniae (CRKP), constitutes an urgent threat to global public health due to bacterial resistance development and the lack of effective vaccines. Outer membrane proteins (OMPs) are important molecules that interact with the surrounding environment and are involved in many biological processes including transport of nutrients and molecules, playing a significant role in antibiotic resistance and enhancing organism virulence. This preliminary study aims to characterise the OMPs of carbapenemase-producing CRKP (CP-CRKP), non-carbapenemase-producing CRKP (nCP-CRKP) and carbapenem-susceptible K. pneumoniae (CSKP) clinical isolates using 1D-SDS-PAGE and liquid chromatography tandem-mass spectrometry (LC-MS/MS) approach. Three K. pneumoniae representative isolates from the predominant sequence type (ST) ST307 CP-CRKP (n=1), hypervirulent ST23 nCP-CRKP (n=1) and CSKP (n=1) were obtained from previous study. The OMPs were extracted by sarkosyl solubilization method followed by protein separation by 1D-SDS-PAGE and protein identification by LC-MS/MS. Subcellular localisation of the identified proteins were analysed using bioinformatic tools. A total of 121 proteins were identified, consisting of 11 OMPs, 10 periplasmic proteins, 65 cytoplasmic proteins, 30 inner membrane proteins and five unknown proteins. Among these, 11 identified OMPs were associated with transport activity (7 proteins), catalytic activity (3 proteins) and an unknown function (1 protein). OmpK36 and lipoproteins were the common OMPs present in all isolates. However, OmpK35 and OmpK37 were not observed in the CSKP isolate. Only 11 out of 121 (9.09%) proteins were identified as OMPs and OmpK36 were found to be conserved in all isolates. The current findings might provide insights into the antibiotic uptake through the major proteins and their importance in antimicrobial resistance in clinical strains, emphasizing their potential importance in therapeutic strategies. Keywords: Carbapenem resistant Klebsiella pneumoniae, outer membrane proteins, proteomic analysis 46
Presenter’s ID: OA09 Category: Therapeutic and Diagnostic SINGLE NUCLEOTIDE POLYMORPHISMS IN DENGUE VIRUS ISOLATE FROM CLINICAL SAMPLES IN MALAYSIA Jun Hao Chiew , Fadhilah Binti Moh Djamil , Hui Yee Chee , Zulkefley Bin Othman Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia. *Corresponding author email address: [email protected] The escalating number of dengue cases posed a significant public health threat, necessitating a comprehensive understanding of the genetic variations in the dengue virus (DENV) and their correlation with disease dynamics. Within this context, single nucleotide polymorphisms (SNPs) diversity played a pivotal role in shaping viral fitness and pathogenesis. Notably, the poorly annotated genomes of DENV, along with heterogeneity among strains, created a dynamic landscape influencing viral genetic diversity and fitness. Investigating SNPs complexity within (intra-host) and between (inter-host) infections was proposed to offer novel insights into virus evolution and enhance virological surveillance. The research aimed to analyse dengue virus genetic variations isolated from clinical samples. This study used archived clinical samples of dengue-positive patients from Hospital Serdang. Isolation of dengue virus from serum samples was performed on Vero cells. Dengue viral RNA from each passage (P) was extracted using the QIAamp Viral mini kit, and real-time Multiplex RT-PCR was used to identify the serotype of the dengue virus. Purified RNA from P2 viral isolates was submitted for whole genome sequencing. Single-nucleotide variants (SNV) were identified based on the bioinformatics report provided. Genomic analysis revealed that a DENV1 strain isolated from a clinical sample exhibited a total of 734 single nucleotide polymorphisms (SNPs). Substantial variations were predominantly observed within key viral proteins, notably the E (112 SNPs), NS3 (131 SNPs), and NS5 proteins (185 SNPs). The results highlighted the presence of genetic variability, particularly within the E, NS3, and NS5 proteins of DENV-2, which are crucial for viral entry, replication, and immune modulation. This indicated the importance of these three viral proteins in dengue viral infection and pathogenicity. Further functional studies aimed at elucidating the specific roles of these variants are imperative for a comprehensive understanding of dengue virus pathogenicity during the outbreak. Keywords: Dengue virus (DENV), single nucleotide polymorphisms (SNPs), genetic variability 1* 2 2 1 2 1 47
Presenter’s ID: OA10 Category: Therapeutic and Diagnostic SEROTYPE DISTRIBUTION OF DENGUE VIRUS IN SAMPLES COLLECTED FROM TWO HOSPITALS: HOSPITAL TUANKU JA’AFAR SEREMBAN AND HOSPITAL CYBERJAYA Nur Aida Binti Mohamad Shah , Fadhilah Binti Moh Djamil , Hui Yee Chee , Nurshahira Binti Sulaiman Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. *Corresponding author email address: [email protected] Dengue infection due to dengue virus (DENV) transmission through Aedes mosquitoes has emerged as a global health concern over the past decades. Four distinct serotypes; DENV1, DENV-2, DENV-3 and DENV-4 have been reported in Malaysia. The prevailing serotypes correlate with increased case numbers, with the possibility of serotype shifts over time. Monitoring DENV serotype prevalence aids in managing specific clinical manifestations observed in infected patients. This study aims to identify the prevalent serotype of the DENV from clinical specimens collected from the study sites using molecular techniques. Eighty-six clinical samples confirmed with NS1 antigen of DENV were collected from Hospital Tuanku Ja’afar Seremban (HTJS) and Hospital Cyberjaya (HCJ). The dengue viral ribonucleic acid (RNA) was extracted from the samples of plasma or serum using the QIAamp Viral RNA Mini Kit (Qiagen). Subsequently, dengue serotypes were identified using multiplex real-time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Dengue viral RNA was detected from 86 samples where 52 samples (60%) exhibited single infections, 17 samples (20%) presented concurrent infections with dual DENV serotypes and 17 samples (20%) tested negative for dengue virus RNA. Only three serotypes, namely DENV-1, DENV-2 and DENV-4 were identified in the study samples. The distribution of single infection across the two hospitals was similar, with DENV-2 being predominant, followed by DENV-4 and DENV-1. Notably, DENV-2 infected patients were more frequently present with persistent vomiting, loose stool, lethargy and myalgia. The preliminary study indicated the possibility of a serotype swift from DENV-4 in 2022 to DENV-2 in 2023 and 2024. Additionally, serotype distribution is not affected by geographical factors in this study’s cohort. The absence of DENV-3 in the study population may be attributed to epidemiological factors influencing the spread of serotypes and warrants the need for more samples to be screened. Comprehensive and active surveillance is crucial for promptly detecting serotype changes and implementing serotype-specific clinical manifestation strategies to reduce the burden of dengue fever in endemic regions. Keywords: Dengue virus (DENV), real-time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), serotype 48 1 2 1 1* 2 2
Presenter’s ID: OB01 Category: Non-Communicable Diseases ELUCIDATION OF Limosilactobacillus reuteri 29B (L29B) ANTI-INFLAMMATORY PROPERTIES IN HCT 116 CELL LINE Loi Yong Xin , Melati Binti Khalid , Leslie Than Thian Lung Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. *Corresponding author email address: [email protected] Inflammatory disorders in the gastrointestinal tract, especially inflammatory bowel disease, are a global health concern. Probiotics promote gastrointestinal well-being through modulation of factors responsible for inflammatory responses and consequently provide relief to the host. Previous studies have shown that probiotics exhibited antiinflammatory potential through the presence of the viable probiotics cell or production of metabolites that downregulate the expression of prostaglandin E2 (PGE2) in human epithelial, suggesting anti-inflammatory activity. The strain Limosilactobacillus reuteri 29B (L29B) has been proven to possess anti-microbial properties and is safe for human use. In this study, the strain will be evaluated for its anti-inflammatory effects through testing in HCT 116 cells. This study aims to investigate the anti-inflammatory properties of L29B using HCT 116 cell line, which is primarily used as a model of the intestinal epithelial barrier. HCT 116 cells will be cultured and exposed to L29B. HCT 116 cells will be treated with lipopolysaccharide (LPS) which is used to induce inflammation by triggering an immune response through releasing of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). Pro-inflammatory cytokine expression levels will be assessed with real-time PCR assay. Cell viability assay of the cell line upon treatment with L29B will be assessed using MTT assay by measuring the absorbance level. Intracellular ROS production levels will be measured by detecting the fluorescence intensity of the oxidant-sensitive probe 2′, 7′- dichlorofluorescein-diacetate (DCFH-DA). For MTT Assay, HCT 116 cell reveals a significant decrease in cell viability following L29B treatment compared to the control group. This observation suggests a potential therapeutic efficacy of L29B in inhibiting the proliferation of HCT 116 cells, highlighting its promise as a candidate for further investigation in anti-inflammatory therapy. In conclusion, L29B treatment significantly decreased the viability of HCT 116 cells compared to controls. Keywords: Anti-inflammatory, colon cancer cell, probiotic 49 2 1 1 1* 2