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NAPA Book- 63位講者 12.10

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Published by youcheng524, 2018-12-10 19:13:50

e-NAPA2018 BooKlet

NAPA Book- 63位講者 12.10







DATE: DEC 17 – 19, 2018

Hotel National, Taichung, Taiwan

NAPA 2018


Monday - December 17, 2018

Session-1: Cancer (Precision Medicine) (10:00-12:00)

Chairs: Dr. Young-Joon Surh & Dr. Chin-Kun Wang

1. Dr. Young-Joon Surh, Collage of Pharmacy, Seoul National University (Republic of
Modulation of tumor microenvironment by bioactive natural products

2. Dr. Johji Inazawa, Kyoto Prefectural University of Medicine and Tokyo Medical and
Dental University (Japan)
Exploring novel cancer-related microRNAs and their diagnostic and therapeutic
potentials in Personalized Cancer Medicine (PCM)

3. Dr. Akira Murakami, University of Hyogo (Japan)
Phytochemicals may exhibit bioactivities via hormesis: roles of proteo-stress and

4. Dr. Danny N. Dhanasekaran, Stephenson Cancer Center (USA)
Targeting Cancer Metabolism for Ovarian Cancer Therapy---Nutrition-based
intervention for metabolic vulnerabilities

5. Dr. Chinthalapally V. Rao, University of Oklahoma Health Sciences Center (USA)
Targeting Precursor Lesions of Pancreatic Cancer Prevention-targeting PanINs and

1! 3

NAPA 2018

Dr. Jian-Bo Xiao,

Professor, Institute of Chinese Medical Sciences,
State Key Laboratory of Quality Research in Chinese Medicine,
University of Macau, Macau, China.
Email: [email protected]

Metabolites of polyphenols in cancer cells

Jian-Bo Xiao*

1 Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese
Medicine, University of Macau, Taipa, Macau, China


Dietary polyphenols are most important phytochemicals in our diets. The function,
bioactivities, and pharmacology of polyphenols have been widely investigated. We have
comprehensively explored the structure-stability relationship of natural polyphenols in
DMEM medium without cells. Polyphenols with catechol or pyrogallol structure were
evidently instable in DMEM medium without cells. We further investigated the metabolites
of quercetin and luteolin when incubated with several cells (cancer and normal) at 37 °C in
5% CO2 for 48 h. The metabolites of quercetin were found to be the glucuronide, sulfate and
methylate of its oxidized quinone. The metabolites of luteolin were found to be its
glucuronide, sulfate and methylated form. The metabolites were quite different from different
cells. We further investigated what really happened for polyphenols in A549 cell, HepG2 cell
and Caco-2 cell.

Acknowledgements: This work was financially supported by Multi-Year Research Grant of
University of Macau (MYRG2018-00169-ICMS) and Start-up Research Grant from
University of Macau (SRG2015-00061-ICMS-QRCM).

Keywords: Polyphenols, metabolites, quercetin and luteolin, cancer cells.


NAPA 2018

Dr. Chin-Kun Wang,

Professor, Chung Shan Medical University,
Taichung, Taiwan
President of ISNFF/NST, Fellow of IUFOST,
Email: [email protected]

Helicobacter pylori-induced cancer and food bioactives

Chin-Kun Wang

Former President, Chung Shan Medical University (CSMU)
Honorary President, Nutritional Society of Taiwan


Burdock complex constituted by burdock (Arctium lappa), angelica (Angelica sinensis),
gromwell (Lithospermum erythrorhizon) and sesame (Sesamum indicum) oil, which are
commonly used in Chinese medicine. The inhibitory and preventive effects of these herbs on
H. pylori remain unknown. The aim of this study was to use clinical trial to investigate the
inhibition of H. pylori infection by the supplement of burdock complex and cell model to
understand the detailed mechanism. H. pylori were measured by C13- urea breath test (UBT).
The H. pylori positive subjects (UBT> 10‰, n= 36) were recruited. Burdock or placebo was
taken for 8 weeks and 2 weeks of follow-up period after administration. The UBT, blood
sample, antioxidant capacity, total phenol, and inflammatory markers were analysed at the
initial, 4th, 8th and 10th weeks. In cell model, the AGS cell was treated with burdock complex
extract and H. pylori to investigate minimum inhibition concentration, cell viability and anti-
adhesion. UBT value and ulcer problems were significantly improved in burdock group as
compared with base line. Moreover, anti-oxidant activity and inflammatory markers (CRP,
IL- 8, TNF- α, and IL- 1β) were greatly reduced. In the cell model, burdock complex could
not suppress the growth of H. pylori but inhibit the adhesion of H. pylori to AGS cell and
interfered the Cag A secretion and IL-8 formation. The other food bioactives including
propolis, noni, Ixeris chinensis also showed excellent function on gastrointestinal tract,
especially on the prevention and treatment of digestive cancer.

Keywords: Food bioactives, Helicobacter pylori, C13- urea breath test


NAPA 2018

Dr. Zhimin Xu,


School of Nutrition and Food Sciences,

Louisiana State University, USA.

Email: [email protected]

Phytochemical Compositions and Anti-cancer Activities of Butterfly Pea
(Clitoria Ternatea) Seed and Petal

Zhimin Xu,

School of Nutrition and Food Sciences, Louisiana State University, USA


Butterfly pea (Clitoria ternatea) is a member of Fabaceae family and Papilionaceae sub-
family. It has been reported to possess health promoting functions (owing to rich in
anthocyanins) including anti-diabetes, anxiolytic, anti-convulsant, antipyretic, anti-
inflammatory and analgesic functions. In this study, lipophilic and hydrophilic extracts of
butterfly pea seeds and petals were extracted by non-polar and polar solvents, respectively.
The hydrophilic phenolics, lipophilic tocopherols, phytosterols and fatty acids in seeds and
petals were identified and quantified by HPLC, LC-MS or GC method for exploring and
understanding the potential bioactive composition and distribution in butterfly pea. Fifteen
antioxidant phytochemicals were found in the seeds. Among them, sinapic acid, epicatechin
and hydroxycinnamic acid derivative were above 0.5 mg/g of fresh weight. The petals had a
group of ternatins, flavone glycosides and delphinidin derivatives. Both of the seeds and
petals contain four phytosterols and alpha- and gamma- tocopherol. The level of beta-
sitosterol or gamma-tocopherol in the seeds was much higher than in the petals. Linoleic
acid was the most abundant fatty acid in the seeds and petals, while phytanic acid was only
found in the petals. The capabilities of lipophilic and hydrophilic extracts of the seeds and
petals in inhibiting HEp-2 human carcinoma cell viability were evaluated in this study as
well. The inhibitory capability of seed or petal hydrophilic extract was much higher than
either seed or petal lipophilic extract, while the capability of seed hydrophilic extract was
significantly higher than the petal hydrophilic extract. The results of this study indicate that
butterfly pea seed and petal are rich in different antioxidant phytochemicals and could be
utilized as a health promoting food ingredient or supplement for the prevention of cancer

Key words: phytochemicals; ternatins; tocopherols; phytosterols; lipids; antioxidants


NAPA 2018

Dr. Li-Shu Wang,

Associate Professor,

Department of Medicine,

Medical College of Wisconsin, USA.

Email: [email protected]

Are we ready for precision medicine using berries?

Pan Pan, Hayden Krause, Arielle Baim, Lyndsey Runaas, Laura Michaelis,

Ehab Atallah, Li-Shu Wang

Division of Hematology and Oncology, Department of Medicine, Medical College of
Wisconsin, Milwaukee, Wisconsin, USA


Familial adenomatous polyposis (FAP) is characterized by the early onset of colonic
polyposis and a high risk for colorectal cancer. Our group conducted a human clinical study
to determine whether black raspberries (BRBs) might regress rectal polyps in patients with
FAP. Fourteen patients with FAP were treated with BRBs daily for 9 months. Despite the
encouraging finding that a 9-month BRBs intervention decreased polyp burden in 11 of 14
patients with FAP, 3 patients were non-responders. Mechanistic studies suggest that non-
responders were resistant to BRBs-induced hypomethylation. Myelodysplastic syndromes
(MDS) are a group of bone marrow disorders characterized by progressive cytopenias and
progression to acute myeloid leukemia. Hypomethylating agents (HMAs), such as azacitidine
and decitabine, are FDA-approved therapies for MDS patients. Approximately 50% of MDS
patients respond to HMAs. Although both azacitidine and decitabine are effective therapies
for some MDS patients, they are associated with significant toxicities that place a large
burden on MDS patients and affect their quality of life. We conducted a pilot clinical trial to
evaluate the hypomethylating properties of BRBs in patients with low-risk MDS. Study is
still ongoing, we have recruited 16 patients. Data from 7 patients suggested that 3 months of
BRB intervention induced hypomethylation in 6 of 7 patients with MDS, 1 patient was a non-
responder. Therefore, our data suggest that changes of methylation may be used as indicators
for responsiveness to BRBs intervention that warrants investigation in a larger clinical trial.

Keywords: Familial adenomatous polyposis, black raspberries, hypomethylation,
Myelodysplastic syndromes.


NAPA 2018

Session-4: Nutrition/Pharmacology/Physiology

Chairs: Dr. Gow-Chin Yen & Dr. Hye-Kyung Na

1. Dr. Gow-Chin Yen, National Chung Hsing University (Taiwan)
Effect of the camellia oil on the alleviation of aluminum-induced cognitive decline

2. Dr. Hye-Kyung Na, Sungshin Women’s University (Republic of Korea)
An alcohol-derived metabolite salsolinol induces hepatic damage in male mice

3. Dr. Zhizhuang Joe Zhao, Stephenson Cancer Center and University of Oklahoma Health
Sciences Center (USA)
Polyphenolic Antioxidants Sensitize the Inactivation of Tyrosine Phosphatases by UV

4. Dr. Shutao Liu, Fuzhou University (China)
In Vivo Bioactivities of Oral Administration of Superoxide Dismutase and Its Possible

5. Dr. Kuan-Chen Cheng, National Taiwan University (Taiwan)
β-Glucosidase for Mogrosides Deglycosylation from Lo Han Kuo

3! 2

NAPA 2018

Dr. Yong Li,

Associate Professor,

Faculty of Medicine,

UNSW Sydney, Australia.

Email: [email protected]

Isolation and Analysis of Extracellular Vesicles as Circulating Biomarkers
for Prostate Cancer Diagnosis

Duojia Wu1,2, Ying Zhu1,2, Jie Ni1,2, Bairen Pang1,2, Julia Beretov1,2, Joseph Bucci1,2, David

Malouf1,2, Peter Graham1,2, Yong Li,1,2*

1Cancer Care Centre, St George Hospital, Kogarah, NSW 2217, Australia 2St George and
Sutherland Clinical School, UNSW Australia, Sydney, NSW 2052, Australia.


Prostate cancer (CaP) is a major health problem in western countries. Extracellular vesicles
(EVs) contain pathogenic components, such as proteins, DNA fragments, mRNA, non-coding
RNA and lipids, and play a critical role in intercellular communication. EVs hold promise for
the discovery of liquid biopsy-based biomarkers for CaP diagnosis. The objective of this
study is to discover biomarkers from cancer-derived EVs in CaP cell lines, blood and urine
using a number of new techniques for CaP diagnosis. EVs were isolated from precipitation
reagent, ultracentrifugation (UC), polymer precipitation (PP) size exclusive chromatography
(SEC), and confirmed by Cryoelectron microscopy (Cryo-EM) for morphology and
nanoparticle tracking analysis (NTA) for size distribution and concentration. High-throughput
LC-MS/MS-based label-free quantification was performed to reveal protein profiling of
urinary exosomes in CaP patients and normal controls, followed by systematic comparison of
differentially expressed proteins between the groups. We studied EVs by a series of isolation
and analysis techniques. These preliminary results are promising and are the technique
fundamentals for our ongoing research by using EVs for liquid biopsy-based CaP early
detection. Our findings also demonstrate that urinary exosomes harbour informative proteins
that might be used for the detection of CaP or monitoring its progression through a non-
invasive way.

Keywords: prostate cancer, extracellular vesicles, exosomes, diagnosis

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