POPE 4023 PRINCIPLES OF ASEPTIC AND STERILE TECHNIQUE (B)

POPE 4023 THE PERIOPERATIVE ENVIRONMENT

PRINCIPLES OF SURGICAL
ASEPSIS AND STERILE
TECHNIQUE

SITI ZAIRINI AHMAD
ILKKM KUBANG KERIAN

LEARNING OUTCOMES

At the end of this learning session, the students will be
able to
1. Explain standards precaution to prevent infection
2. Discuss the sterile technique – level of sterility and

disinfection
3. Describe the principles of aseptic technique

STANDARD PRECAUTION

Protective barriers and personal protective equipment (PPE)
Prevention of puncture injuries
Management of puncture injuries
Oral procedures

STANDARD PRECAUTION

Care of specimens
Decontamination
Laundry
Waste

STANDARD PRECAUTION

Handwashing
No touching of mucous membranes
Prophylaxis

SPAULDING ‘S LEVEL

NONCRITICAL SEMICRITICAL CRITICAL

Contact with Contact with Entering the
only intact intact skin or bloodstream,
body tissues
skin or mucous and mucous
mucous membranes membranes
membranes
Sterility is not Sterility is
Only clean and maintained during compulsory
unsterile
storage or use

PRINCIPLES OF STERILE AND ASEPTIC
TECHNIQUE

1 Only sterile items are used within the sterile field

2 Sterile personnel are gowned and gloved

3 Tables are sterile only at table level

4 Sterile personnel touch only sterile items or areas;
Unsterile personnel touch only unsterile items or area

PRINCIPLES OF STERILE AND ASEPTIC
TECHNIQUE

5 Unsterile personnel avoid reaching over the sterile field;
Sterile personnel avoid leaning over an unsterile area

6 The edges of anything that encloses sterile contents
are considered unsterile

7 The sterile field is created as close as possible to the
time of use

8 Sterile areas are continuously kept in view

PRINCIPLES OF STERILE AND ASEPTIC
TECHNIQUE

9 Sterile personnel keep well within the sterile area

10 Sterile personnel keep contact with sterile areas to
a minimum

11 Unsterile personnel avoid sterile areas

12 Destruction of the integrity of microbial barriers
results in contamination

PRINCIPLES OF STERILE AND ASEPTIC
TECHNIQUE

13 Microorganism must be kept to an irreducible
minimum