Knockout vs Overexpression Stable Cell Lines

META: AcceGen shares details about the differences between knockout stable cell lines and
overexpression stable cell lines to help you decide which is best.

(H1) Knockout vs. Overexpression Stable Cell Lines

Stable cell lines are an important research tool used by pharmaceutical companies, for gene
therapy research, and for compounding research. Each stable cell line is constructed using
various methods to determine whether it will be a knockout cell line or an overexpression cell
line.

The process uses do vary but, in general, stable cell lines are constructed by integrating the
exogenous plasmid DNA into a host cell chromosome so that the host cell can express the
protein of interest for a longer period. The exogenous is capable of being stably expressed in cell
division for as long as two weeks.

(H2) What Is the Benefit of Using Stable Cell Lines?

Using stable cell lines helps improve screen processes to determine the appropriate number of
cells required for various compounds and pharmaceutical drugs. In addition, stable cell lines can
help reduce costs since the use of excessive conversion of dyes and virus packaging can be
greatly reduced.

Furthermore, experiments and research are better facilitated. One can also achieve better genetic
interference effects using established stable cell lines. Last, using stable cell lines can further
reduce and effectively eliminate the interference of individual differences in the experimental
results.

(H2) What Are Knockout Stable Cell Lines?

Knockout stable cell lines are where the CRISPR/Cas9 system is used to knock out one or double
genes to generate and create a knockout cell line. Using the CRISPR/Cas9 system has become
one of the most popular methods for stable cell line generation since knockout cell lines require
genome editing.

It is faster, cheaper, and more accurate than previous techniques of editing DNA and has a wide
range of potential applications. These cell lines provide you with a convenient and efficient way
to research and study various gene functions, including potential mutant gene functions.

(H2) What Are Overexpression Cell Lines?

Overexpression cell lines are widely used for protein engineering, drug discovery, recombinant
antibodies development, biology research, and other such research and experimentation.

The gene of interest is integrated into the host cell after it is transduced and permanently passed
to the next cell generations where it expresses the protein of interest. The common method for

stable cell generation of overexpression cell lines requires focusing on the gene of interest, also
called the GOI.

To achieve the desired GOI, plasmid transfection or lentivirus transduction is applied to the cell
line. Lentivirus is used to infect cells because it is a broad tropism that can target most
mammalian species and cell types.

(H2) Which Stable Cell Line Is Best?

Knockout cell lines and overexpression cell lines serve different purposes. As such, you need to
decide what type of research and experimentation you are conducting to determine which cell
line would best meet your needs and requirements.

For example, a knockout CRISPR stable cell line would be better suited when you want to edit
genomes and to study specific gene functions. On the other hand, overexpression cell lines would
be better when you want to focus on a specific GOI.

(H2) About AcceGen

AcceGen is a leading U.S. biotechnological company founded in 2016. Located in Fairfield, NJ,
AcceGen provides complete and authentic cell products and services for pharmaceutical
companies, scientific research studies, compounding research, and more.

We provide access to high-quality Primary Cells, Tumor Cell Lines, MicroRNA
Agomir/Antagomir, Stem Cells, Immortalized Cell Lines, Transfected Stable Cell Lines, and
Nucleic Acid Kits.

We develop innovative technologies for our product lines and services like our stable cell lines
and custom stable cell line services. Utilizing our unique processes, we construct your cell line
based on your criteria.

Next, we perform transfection or transduction on target cells and select the desired stable cell
clones. Finally, we perform a gene expression evaluation to ensure the newly created stable cell
line meets your requirements. We also supply supporting data for our custom stable cell lines.

We are committed to supplying the best products and services to our customers to further
medical, drug, and scientific research for enhancing the overall quality of life. For further
information about our products and services, please feel free to visit our official website at
www.accegen.com or contact us at 862-686-2696 today!