Microbiological Test Report

LAB TEST REPORT
MRSA





LAB TEST REPORT
MRSP

BACTERIOLOGY TEST REPORT O
O
Lab Case Number : B2050/2012
Date samples completed : 04/ 12/2012 UniVGISity of Pretoria
Date of report : 07/12/2012
Attention : John Temperley
Client / Owner :
Health and Hygiene

Niethicillin Resistant S.pseudintermedius laboratory isolate B892/2012 sensitivity against

F10 SC veterinary disinfectant: Batch nr 141018.

Dilution Plate 1 (mm) Plate 2 (mm) Average (mm)

1/ 125 9.89 9.89 9.89

1/250 8.11 8.67 8.39

1/ 500 7.93 8.43 8.17

1/ 1000 6.64 7.33 6.99

1/2000 0 0 0

1/4000 0 0 0

1/8000 0 0 0

1/ 16000 0 0 0

Control (Plain filter paper 0 0 0
disk)

Comments:

* The results provided in the report are only relevant to the laboratory samples as received and analysed by the
laboratory.

* This test report shall not be reproduced except in full without written approval of the laboratory.

* Opinions and interpretations of test results fall outside the scope of accreditation. Should you have enquiries,
please do not hesitate to contact us for more information / on the above-mentioned results.

ro Michel B2050/2012

pecialist

Page 1 of 2

LAB TEST REPORT
Aspergillus

TEST REPORT 8 88

MICROBIOLOGY DEPARTMENT 7218

Health & Hygiene U verw/Your ref: O/N SO60
Attention: Mr JP Temperley ons verwidur ref: 17/37/9
P.O.Box 347 Navrae/Enquiries: 428-6087
SUNNINGHILL
Datuminate: 2oos-o2-o4
2157
1733791/04-2186/X 316280
Bladsy/page 1 vaulof 2

DISINFECTANT EFFICACY TEST - EN 13697-2001 THIS TEST HAS BEEN [
CARRIED OUT USING A
I

FIO "SUPER CONCENTRATE"

SAMPLE

1. DESCRIPTION OF SAMPLE

One sample labelled "F10SC B/N 211" was received on 6/7/2004 and tested on 9/7/2004.

2. TESTS REQUESTED

Fungicidal activity of the chemical disinfectant using Aspergillus niger spores as test organism.

3. METHOD OF TEST
The sample was tested in accordance with EN 13697 - 2001 Specification for Disinfectants.

3.1 A test suspension of fungal spores in solution of interfering substance, simulating clean conditions,
added to a preparation sample of the product under test diluted in hard water.

3.2 The mixture is maintained at 200C + loC for 5 minutes and 15 minutes + 10 seconds.

3.3 At this contact time an aliquot was taken and the fungicidal action is immediately neutralized using a suitable
neutralizer.

3.4 Test spore suspension Aspergilhts niger ATCC 16404.

Spore suspension requirement:

The number of spores in the test suspension adjusted to 1,5 x 10 ' to 5 x 10 * efu/ml .

Suspension maintained in a water bath at 200C + loC (use within 2 hours)

4 Results / ..

1 Dr Lategan Road Greenkloof, Private Bag XI91 Pretoria 0001, Tel: + 27 (012) 428-7911, Fax: + 27 (012) 344-1568.
This test was performed by Testing and Conformity Services (Pty) Ltd, an affiliate of the SABS.
This report relates only to the specific sample(s) tested as identified herein. It does not imply SABS approval of the quality and/or performance of
the item(s) in question and the test results do not apply to any similar item that has not been tested.
(Refer also to the complete conditions printed on the back of the official test reports.)

SABS test report 1733791/04-2186/X31628c Page 2

REPORT No.

3.5 Hard Water - anhydrous magnesium chloride, anhydrous calcium chloride and sodium bicarbonate.
3.6 Interfering substance - 1% Skimmed milk

4. RESULTS

Fungicidal activity of F10SC (B/N 211)

Sample Dilution Contact Direct Aspergillus niger spores

time

F10SC 1/50 5min Disc 111 64 99,99

B/N211

(X31628) 77 - 65 99,99

1/50 15 min Disc

1/ 100 5 min Disc 88 69 99,99

1/100 15min Disc 60 67 99,99

Spore suspension:

Inilial spore suspension count - 1,6 x 10 * efu /ml (within the requirement)

Conclusion:

Quantitative non porous surface test (disc) for the evaluation of fungicidal activity of chemical disinfectants used in
the food , industrial, domestic and institutional areas - without mechanical action for which a 10 (99,99%) or more

reduction in viability is demonstrated under required test conditions.

MA KERR RA ROOS
TEST OFFICER: MICROBIOLOGY MANAGER: MICROBIOLOGY

Fax: (011) 474 1670

Confidentiality Notice:
Trd1eh9eli9ilasi3vn,emcrietienscgsooanntghteaitshineisms eciinnostfsneoatnregmdneetastdotioootnhnfeltythhinaifsotterisnctdohsemetrdmiucrstueleynciiocpcaoifetntinhoftein,d,eyinonidsutiivasaildtrreIuicfahttlehlyeroeprrberyeoanhdnteioibtrytiitofeitefodtd.hwitIshhfaimcytheoasunistyahigsdaeivsaiecsdldonrresoeuctsresteheiv,ededrdaiesncttdrhipibiisineuntcittoeo,nrmom,rmscthouoepnfyieciSnmaegtpicoltooinorynetihne3e2oetrarorakofgirnt,ehgneptolerSfeatsaaspennoydnnaarsodcitbstiifloeyAnfcouitns,r

immediately by telephone to arrange for the return of the documents to us.

This report relates only to the specific sample(s) tested as identified herein. It does not imply SABS approval of the quality and/or performance
of the item(s) in question and the test results do not apply to any similar item that has not been tested. (Refer also to the complete conditions
printed on the back of official test reports.)

LAB TEST REPORT
Avian Flu

















LAB TEST REPORT
Bacillus

TH Hd 88

TEST REPORT

MICROBIOLOGY DEPARTMENT 7218

Health & Hygiene U verwfYour ref: e-M 4/ 10/2004
Attention: Mr JP Temperley
P.O.Box 347 ons verw/otr ref: 17/37/9
SUNNINGHILL
2157 Nayme/Enquiries: 428-6087

Datum/Date: 2005-o2-o4

1733791/04-3294/X38022-3
Bladay/page 1 van/of 2

DISINFECTANT EFFICACY TEST - EN 13704 - 2002 THIS TEST HAS BEEN
1. DESCRIPTION OF SAMPLES
CARRlED OUT USING A
FIO "SUPER CONCENTRATE"
SAMPLE

Two samples labelled "F10SC B/N 050506 & F10SC B/N 211" were received on 7/10/2004 and tested
on 13/10/2004 and 19/10/2004.

2. TESTS REQUESTED

Sporicidal activity of the chemical disinfectant using Bacilh<s subtilis spores as test organism.

3. METHOD OF TEST
The samples were tested in accordance with EN 13704-2002 Specification for Disinfectants.

3.1 A test suspension of bacterial spores in solution of interfering substance, simulating clean conditions,

added to a preparation sample of the product under test diluted in hard water. (Dilution 1/100 )

3.2 The mixture is maintained at 20aC & loC for 5 minutes & 10 seconds.

3.3 At this contact time an aliquot was taken and the sporicidal action is immediately neutralized using a suitable
neutrahzer.

3.4 Test spore suspension Bacillus subtilis ATCC 6633.

Spore suspension requirement:

The number of spores in the test suspension adjusted to 1,5 x 10 * to 5 x 10 'cfu/ml .
Suspension maintained in a water bath at 200C & loC (use within 2 hours)

4 Results / ..

1 Dr Lategan Road GroenIdoof, Private Bag X191 Pretoria 0001, Tel: + 27 (012) 428-7911, Fax: + 27 (012) 344-1568.
This test was performed by Testing and Conformity Services (Pty) Ltd, an affiliate of the SABS.
This report relates only to the specific sample(s) tested as identified herein. It does not imply SABS approval of the quality and/or performance of
the item(s) in question and the test results do not apply to any similar item that has not been tested.

(Refer also to the complete conditions printed on the back of the official test reports.)

SABS test report 1733791/04-3294/X38022-3 Page 2

REPORT No.

3.5 Hard Water - anhydrous magnesium chloride, anhydrous calcium chloride and sodium bicarbonate.
3.6 Interfering substance - 1% Skimmed milk

4. RESULTS

Sporicidal activity of F10SC (B/N 050506 and BIN 21 1)

Sample Dilution Contact Serial Percentage kill of
time dilution II. subtilis spores

Fl0SC 1/ 100 5min Direct 99,74 99,80
B/N 050506 10-1 99,86 99,98
10-2 99,99 99,99
(X38022) 10-3 99,99 99,99

FlOSC 1/100 5min Direct 99,97 99,97
B/N 211 10~ 99,99 99,99
10-2 99,99 99,99
(X38023) 10- 99,99 99,99

Spore suspension:

Initial spore suspension count - 2,85 x 10 * efu Iml (within the requirement)

Conclusion:
Sporicidal activity for specific purpose is characterized by the concentration of the test product for which criteria

are met for which a 103 (99,9%) or more reduction in viability is demonstrated under required test conditions.

MA KERR 1 RA ROOS
TEST OFFICER: MICROBIOLOGY MANAGER: MICROBIOLOGY

Fax: (011) 474 1670

Confidentiality Notice:
This message is intended only for the use of the individual or entity to which it is addressed and in terms of Section 32 of the Standards Act,
1993, it contains information that is strictly confidential. If the reader of this message is not the tweipient, or the employee or agent responsible for
delivering this message to the intended recipient, you are hereby notified that any disclosure, distribution, copying or the taking of any action in
reliance on the contents of this communication, is strictly prohibited. If you have received this communication in error, please notify us
immediately by telephone to arrange for the return of the documents to us.

This report relates only to the specific sample(s) tested as identified herein. It does not imply SABS approval ofthe quality and/or performance
of the item(s) in question and the test results do not apply to any similar item that has not been tested. (Refer also to the complete conditions
printed on the back of official test reports.)

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LAB TEST REPORT
Stability

(2 years RTU sample tests)

TEST REPORT THHa81a

MICROBIOLOGY DEPARTMENT 7218

Health and Hygiene U verw/Your ref: Verbal o/n 0059
Attention: Mr JP Temperley ons venviour ree 17/37/9
PO Box 347 Nayme/Enquiries: 428-6087
SUNNINGHILL Datum/Date: 2005-04-12
1733791/03-4526/W11421-6b
2157 Bladsy/page 1 van/of 3

STABLITY TESTS (37oC) - READY TO USE 710 SOLUTION

1. DESCRIPTION OF SAMPLES

Four samples labelled "710 B/N 061A; FIO B/N 061B; £ 10 061C and Retention sample £ 10 B/N 061" were
received for bactericidal efficacy testing on the 18 February 2003 and tested on 7, 14, 35 days then at 3 months,
after which monthly to 24 months. A retention sample was tested at 24 months only.
The test samples were incubated at 370C for the duration of the test.

2. TESTS REQUESTED

To determine the bactericidal efficacy of the samples subject to the following conditions:

a) Dilution of samples: "As is"

b) Diluent: Sterile hard water of 250 ppm hardness containing 1%

skimmed milk.

c) Temperature of test: 22 oC

d) Test organisms: Pseudomonas aeruginosa SATCC Pse 16

e) Test organism load: Appmximately 10" organisms per 10 m! of test solution
f) Exposure time:
2 min and 5 min

g) Inactivator: A suitable fluid inactivator

h) Medium: Nutrient Agar

i) Storage Temperature: 370C

3. METHOD OF TEST

The samples were tested in accordance with the method described in SABS 1615-1994, and subject to the conditions

stated in Paragraph 2 above.

1 Dr Lategan Road Greenkloof, Private Bag XI91 Pretoria 0001, Tel: +27 (012) 428-7911, Fax: + 27 (012) 344-1568.

This test was performed by Testing and Conformity services (Pty) Ltd, an affiliate of the SABS.
This report relates only to the specific sample(s) tested as identified herein. It does not
imply SABS approval of the quality and/or performance of
the item(s) in question and the test results do not apply to any similar item that has not been tested.
(Refer also
to the complete conditions printed on the back of the official test reports.)

SABS test report THHa81a

REPORT No. 173379ilo3-04526NV11421-26b Page 2

4 RESULTS - STABILITY TESTS - READY TO USE SOLUTION
Bactericidal efficacy

o

Day 7 99.9 99.9 99.9 99.9 99 9 99.9
Day 14 99.9 99.9 99.9 99.9 99.9 99.9
Day 21 99.9 99.9 99.9 99.9 99.9
Day 35 99.9 99.9 | 99.9 99.9 99.9 99.9
99.9 99.9 99.9 99.9 99.9
Month 3 99.9 99.9 99.9 99.9 99.9 99.9
Month 4 99.9 99.9 99.9 99.9 99.9 99.9
Month 5 99.9 99.9 99.9 99.9 99.9 99.9
Month 6 99.9 99.9 99.9 99.9 99.9 99.9
Month 7 99.9 99.9 99.9 99.9 99.9 99.9
m Month 8 99.9 99.9 99.9 99.9 99.9 99.9
99.9 99.9 99.9 99.9 99.9 99.9
8 Month 9 99.9 99.9 99.9 99.9 99.9 99.9
99.9 99.9 99.9 99.9 99.9 99.9
Month 10 99.9 99.9 99.9 99.9 99.9 99.9
Month 11 99.9 99.9 99.9 99.9 99.9 99.9
Month 12 99.9 99.9 99.9 99.9 99.9 99.9
0 Month 13 99.9 99.9 99.9 99.9 99.9 99.9
2 Month 14 99.9 99.9 99.9 99.9 99.9 99.9
e Month 15 99.9 99.9 99.9 99.9 99.9 99.9
Month 16 99.9 99.9 99.9 99.9 99.9 99.9
Month 17 NT NT 99.9 NT 99.9 99.9
Month 18 NT NT 99.9 NT 99.9 99.9
Month 19 NT NT NT NT 99.9 99.9
Month 20 NT NT NT NT 99.9 99.9
Month 21 NT NT NT NT 99.9 99.9
Month 22 NT NT NT NT 99.9 99.9
Month 23 NT
Month 24 NT
Retention sample

Note: NT = Not Tested
REMARKS:

When tested in accordance with Section 6.3 of SANS 1615-1994, the relevant dilution of the product shall, within 5 min, kill at least 99.9% of the
organisms indicated.

This report relates only to the specific sample(s) tested as identified herein. It does not imply SABS approval ofthe quality and/or performance
ofthe item(s) in question and the test results do not apply to any similar item that has not been tested. (Refer also to the complete conditions
printed on the back ofofficial test reports.)



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