BSHI Conference 2017

StemCell Technologies [Stand 3]

STEMCELL Technologies provides optimized cell separation products and
systems for HLA and chimerism analysis, to facilitate high-volume sample
processing and clear, reliable test results. EasySep™ and RosetteSep™
are fast, gentle and can be used at room temperature. RoboSep™, the
fully automated cell separator, isolates cells with no cross-contamination.

SystemLink [Stand 6]

SystemLink offers HistoTrac - a complete, customizable HLA laboratory
management system. The software uses a flexible design that will meet
the needs of your laboratory. It manages samples and orders on
patients and donors from test requests through reporting of results.
Interfaces provide seamless data entry. Our effort is to provide a system
that uses a single database for all data entry, data lookup, and
reporting.

Takara Bio Europe [Stand 13]

Takara Bio Europe is a wholly owned subsidiary of Takara Bio Inc., who
develops, manufactures, and distributes a wide range of life science
reagents under the Takara™, Clontech®, and Cellartis® brands.

Key products include SMART cDNA synthesis kits for a variety of samples
and applications, including NGS, high-performance qPCR and PCR re-
agents (including the Takara Ex Taq®, Takara LA Taq®, Titanium®, and
Advantage® enzymes), Cellartis stem cells and stem cell reagents, RT
enzymes and SMART library construction kits, the innovative In-Fusion®
cloning system, Guide-it gene editing tools, Tet-based inducible gene
expression systems, and Living Colors® fluorescent proteins.

Recently, Takara Bio acquired Rubicon Genomics and WaferGen Bio-
systems. As part of the Takara Bio family, they expand our NGS portfolio
as well as add R&D, manufacturing, and support for automation sys-
tems for NGS and qPCR applications. Takara Bio’s portfolio supports ap-
plications including NGS, gene discovery, regulation, and function
studies, as well as genetic analysis, protein expression and purification,
gene editing, stem cell studies, and plant and food research. For more
information, visit takarabio.com.

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VHBio [Stand 1]
VH Bio Ltd is a leading supply and distribution company for the life
sciences industry within the United Kingdom and Republic of Ireland with
novel and innovative products sources from our global partners. VH Bio
is the preferred choice for transplant diagnostics, molecular biology, cell
biology and Immunology.
Please visit stand 1 to collect your BSHI mug and discuss all the new
products launched in 2017 from One Lambda which include:
AllType™ offers a complete NGS solution that delivers unambiguous,
high-resolution genotyping results in less than three days. The AllType
workflow offers platform-specific barcoding to allow for added flexibility
and sequencing on your system of choice.
FlowDSA-XM™ advances the science of crossmatch testing by
combining traditional flow cytometry with microbead technology.
Together, these proven technologies selectively distinguish leukocyte
antibodies from autoantibodies, allowing you to obtain selective
detection in the first run.
You can also find out about complement and cell isolation related
products from our long term partner Cedarlane, as well as the new
magLEAD® 6gC & magLEAD® 12gC systems from Precision System
Science Co. (PSS) which offer an easy to use bench top solution for fast,
high-quality, low cost nucleic acid extraction from up to 6 or 12 samples
respectively.

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Posters 1 - 14

Kidney Transplantation in the Presence of
1 Alloantibodies against HLA-DPB1*04:01 in a Patient Mrs Gemma Cutland

with a Null Variant of HLA-DPB1*04:01 Allele

Investigation into the Significance of Antibodies to Mr Waqidul Islam
2 Denatured HLA Class I Antigen on Renal

Transplantation

3 A Cross-Locus Antibody Reactivity Pattern in Miss Victoria Iveson
Antigens Expressing the HLA-Bw4 Epitope

4 Development of a Rapid Protocol HLA antibody Mrs Katherine Cullen
screening method

5 A Novel Biomarker Assay in Post Renal Transplant Mrs Katherine Cullen
Patients

6 Transplanting against donor directed antibodies in Mrs Emma Goulding
a cord blood transplant.

False homozygous HLA typing results due to a loss Mrs Sarah Peacock
7 of heterozygosity in a patient with acute myeloid

leukaemia

Identification of Optimal Short Tandem Repeat

8 (STR) Panels for Post-Transplant Monitoring Dr Leigh Keen
(Chimerism) of Related and Unrelated Donor Stem

Cell Transplants.

9 The Cord Blood Selection Advisory Panel: The first Dr Kay Poulton
26 reviews Dr Katy Latham

10 Unrelated donor selection habits in the UK Dr Laura Ford
Mrs Laila Roberts
11 A Cautionary Typing Tale
Miss Sarah Blow
12 Changes in CBU selection practice in the UK over
the last three years Mrs Kathryn Howson

The effect of MICA and HLA-DPB1 mismatching on
13 the incidence and severity of graft versus host

disease

HLA genotyping by Next Generation Sequencing
14 in the clinical setting: a 9 month review of

performance

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Posters 15 - 28

Implementation of qPCR for deceased donor Sejal Morjaria
15 typing: One year on - the first UK laboratory Dr Katherine Mounsey

experience

16 Rapid HLA Typing Using SmartType™ Real-time
PCR Technology

Extending the sequences of HLA class I alleles

17 without full-length genomic coverage in the IPD- Miss Kylara Hassall
IMGT/HLA Database using Single Molecule Real-

Time (SMRT®) DNA sequencing technology

18 A robust and rapid method for KIR typing using Miss Marie Asselin
KIR LinkSēq™ Miss Nicola Brosnan

19 SMRT® Sequencing - Improving & Optimising the
Pipeline

20 An evaluation of alternative methodologies for Ms Emily Harding
purity assessment of specific cell lineages

The Impact of Introducing a Rapid Flow Miss Deborah Cook
21 Cytometry Crossmatch Method to Routine

Service

22 The use of QTYPE® in reducing HLA typing Miss Marie Asselin
turnaround time in deceased donation

23 A further example of the HLA-B*15 family allele -- Mr Christopher Harvey
B*15:237

24 High-throughput data analysis of over 33,000 Mr James Robinson
samples in a clinical registry setting.

25 Validation of Third Generation Sequencing Mr Arthur W Gymer
Software on the Pacific Biosciences RS II

Determining the length of homopolymer regions Dr Gayle Leen
26 in HLA alleles typed on the Pacific Biosciences RS Dr Jane Matthews
Mrs Deborah Pritchard
II

Suspected lung transplant-related graft-versus-
27 host disease: HLA typing can contribute to

diagnosis

28 The accuracy of HLA typing in the UK and Ireland
for EQA samples 2013-2016

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Posters 29 - 42

UK NEQAS for H&I's Interpretative Educational Mrs Deborah
29 Scheme -- 2016 results from the UK and Ireland H&I Pritchard

Laboratories Mr Robert Bradshaw
Dr Vaughan Carter
30 Case study: antibody-mediated rejection caused Mr Khalifa Ait Said
by sensitisation to Kidd blood group antigen Jka
Miss Emma White
31 Cardiothoracic Transplantation - Introduction of
the Cardiothoracic Advisory Group Guidelines Dr David Turner
Dr Louise Nicol
32 Risk Stratification of Microbead Array Defined HLA Mrs Jennifer Lord
Specific Antibodies
Miss Maame-Eso
Successful de-sensitisation and live donor renal Yeboah
transplantation following a positive complement
33 dependent cytotoxic crossmatch (CDC-XM)
potentially due to pregnancy induced antibodies
-- a Barts Health case study

34 HLA specific antibodies post islet transplantation; a
single centre experience

35 Soluble CD30 as a biomarker of rejection in HLAi
transplants

Deceased donor HLA typing by LinkSēq™
36 facilitates virtual crossmatching in solid organ

transplantation

An audit of the potential benefits of crossmatching
techniques on cold ischaemia times in solid organ
37 deceased donor transplants: recommendations
from an H&I perspective.

38 The Cytotoxic Crossmatch: No longer a necessity? Mr Sean Carey

18 incompatible friends and family: Tackling a Mr Christopher Burke
39 second kidney transplant for a highly sensitised
Mrs Pamela Hughes
patient. Mrs Maureen
Bloxham
40 Leeds Highly Sensitised Patients' Strategy
Miss Maame-Esi
Performing the pre-transplant crossmatch using Yeboah
41 peripheral blood lymphocytes from deceased

donors-Leicester's experience.

An audit of the Out-of-Hours Deceased Donor

42 Typing Pathway: Recommendations from an H&I
perspective.

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Best Abstract Presentations

BA1 Luminosity -- A Tool for Luminex Antibody Data Dr Mian Chen
Visualisation

BA2 HLA-DP Antibody Incompatible Kidney Dr Adrienne Seitz
Transplantation: A Single Centre Review

BA3 Implementation of A Rapid RT-PCR Technique Dr Deborah Sage
for Deceased Organ Donor HLA Typing

BA4 Review of the NHSBT bowel advisory guidelines: Mrs Sarah Peacock
a single centre experience

Mismatched unrelated donor selection for stem Mrs Sarah Peacock
BA5 cell transplantation: Use of immunogenicity

scores to augment donor selection?

Young Scientist Award

Development of a four-colour cytotoxic flow

YS1 crossmatch assay for the discrimination of Mrs Sarah Peacock
complement and non-complement fixing IgG

antibodies.

YS2 Donor specific antibody production after allograft Dr Gaetano Lucisano
nephrectomy.

An evaluation of PAKLx™ -- a Luminex®-based Miss Jenna Bestwick
YS3 assay for the detection of anti-human platelet

antibodies.

Kidney transplant against high titre allele specific Mr Sean Heveran
YS4 antibodies detected by Luminex, but borderline

positive B cell flow cytometry crossmatch

Physiochemical disparity of mismatched HLA

YS5 alloantigens and outcome following allogeneic Mrs Sarah Peacock
haematopoietic stem cell transplantation: its

application in the clinical laboratory.

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Delegate List

Name Affiliation

Mr Jashan Abraham Anthony Nolan Research Institute
Dr Khalifa Ait Said Manchester Royal Infirmary
Ms Afshan Ali Omixon
Miss Deeya Balgobin Viapath Guy's Hopsital
Dr Anna Barker Manchester Royal Infirmary
Miss Jenna Bestwick Welsh Blood Service, WTAIL

Miss Sarah Blow Imperial College Healthcare NHS Trust

Dr Francisco Boix NHS Blood and Transplant

Mr Robert Bradshaw University Hospitals of Leicester

Professor David Briggs NHSBT

Dr Paul Brookes Hammersmith Hospital, Imperial College NHS
Trust

Mrs Jessica Brookes NHSBT

Miss Nicola Brosnan Anthony Nolan Histocompatibility Labs
Dr Colin Brown NHSBT

Mr Tom Browne NHS Blood and Transplant
Mrs Ana Bultitude NHSBT
Mr Christopher Burke Churchill Hospital, Oxford

Ms Maria Burton WTAIL

Mr Sean Carey Plymouth Hospitals

Ms Barbara Cauwelier AZ St Jan Brugge-Oostende

Dr Nezih Cereb Histogenetics

Dr Mian Chen Oxford Transplant Centre

Dr Brendan Clarke Leeds St James University Hospital

Mr David Clegg NHS Blood and Transplant

Ms Rachael Cole Anthony Nolan

Miss Deborah Cook Manchester Royal Infirmary, CMFT

Mr Eamonn Cudworth Anthony Nolan Histocompatibility Laboratories

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Mrs Gemma Cutland Churchill Hospital, Oxford

Miss Paige Davis BSHI

Dr Katy Derbyshire Barts Health NHS trust

Miss Jenny Douglas NHS Blood and Transplant
Mrs Beth DuBerry IBTS

Mrs Sarah Dyer Welsh Blood Service

Mr Daniel Eggleston Manchester Royal Infirmary

Dr Irina Evseeva Anthony Nolan Research Institute

Miss Nicola Firmin Imperial NHS healthcare trust

Mr Patrick Flynn Manchester Transplantation Laboratory

Dr Laura Ford Manchester Transplantation Laboratory

Miss Jennifer Gauss NHSBT Tooting

Mrs Emma Goulding Transplantation Laboratory, Manchester

Mr Arthur W Gymer Anthony Nolan Research Institute

Dr Emily Halford NHSBT Birmingham

Ms Siobhan Hamilton– NHS Blood and Transplant
Jones H&I, NHS Blood & Transplant

Dr Andrea Harmer

Ms Grainne Harmon H&I Lab, Beaumont Hospital

Miss Kylara Hassall Anthony Nolan

Dr Mark Hathaway NHS Blood and Transplant

Mr James Hayhurst Anthony Nolan Research Institute

Mr Daniel Hayward Anthony Nolan Research Institute

Ms Alice Higgins BSHI

Mrs Kathryn Howson Addenbrooke's

Mrs Pamela Hughes St James' University Hospital

Miss Maria Irvine Royal Liverpool University Hospital

Mr Waqidul Islam Viapath Guy's Hospital

Miss Victoria Iveson NHSBT Newcastle

Miss Sinthujah Jeganathan NHSBT

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Dr Leigh Keen H&I Laboratory, NHSBT Filton

Dr Younis Khan Guy’s Hospital

Dr Katy Latham Anthony Nolan

Ms Nina Lauterbach Omixon

Dr Helena Lee Manchester Royal Infirmary, Transplantation
Dr Gayle Leen Laboratory

Anthony Nolan Research Institute

Dr Ann-Margaret Little Gartnavel General Hospital

Mr Dario Lo Sicco Anthony Nolan Histocompatibility
Mrs Jennifer Lord Laboratories

Manchester Royal Infirmary

Dr Gaetano Lucisano Imperial College Healthcare NHS Trust

Professor Steven Marsh Anthony Nolan Research Institute

Dr Jane Matthews NHSBT Newcastle

Dr Neema Mayor Anthony Nolan Research Institute

Miss Ashleigh McBride H&I laboratory Glasgow

Dr Jessica McCappin Belfast City Hospital

Mr Alasdair McWhinnie Anthony Nolan

Dr Christina Navarrete NHS Blood and Transplant

Miss Aisling O’Brien Beaumont Hospital

Ms Agnieszka Ojrzynska Royal Brompton & Harefield NHS
Foundation Trust

Ms Gillian Orford Royal Liverpool University Hospital

Mrs Hina Patel Sheffield H&I Laboratory

Miss Dhara Patel Royal London Hospital

Miss Neuza Pina Royal London NHS Trust

Dr Kay Poulton Manchester Royal Infirmary

Miss Fiona Powell Hammersmith Hospital

Ms Leonie Powley Cambridge University Hospitals

Mrs Deborah Pritchard UK NEQAS for H&I

Ms Julie Purcell H&I Laboratory, Beaumont Hospital, Dublin

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Dr Tracey Rees Welsh Blood Service
Ms Anjali Reuben Leicester General Hospital
Ms Angela Richardson Gartnavel
Mrs Isla Ritchie Leicester Transplant Laboratory
Mrs Helen Roberts WTAIL
Mrs Laila Roberts Anthony Nolan
Dr Victoria Robertson PCI
Mr James Robinson Anthony Nolan Research Institute
Miss Emma Rowe NHSBT
Dr Deborah Sage NHSBT-TOOTING CENTRE
Dr Adrienne Seitz St James's Hospital
Dr Olivia Shaw Viapath, Guys Hospital
Dr John Smith Royal Brompton & Harefield NHS
Foundation Trust
Dr Nicole Stanley Anthony Nolan
Miss Nikoletta Stoch Beaumont Hospital H&I Laboratory
Ms Sarah Stokes Irish Blood Transfusion Service
Mr Franco Tavarozzi Anthony Nolan
Dr David Turner Royal Infirmary of Edinburgh
Dr Sharon Vivers Anthony Nolan
Mr Robert Whittle NHSBT Sheffield H&I Department
Miss Victoria Wood Leeds Teaching Hospitals NHS Trust
Miss Maame-Esi Yeboah Imperial College Healthcare NHS Trust

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