E_IO1 scanvac

EQUIPMENT : FREEZE DRYER, COOLSAFE 110 (SCANVAC)

S/N: 7080037
KOD HARTA: 2009000183

Personal protective Equipment Contact Number
Noor Meliza Jamil
1. Lab coat 019-3744554
2. Proper enclosed footwear
3. Gloves when handling samples
4. Safety Glasses

Potential Hazard & Safety Precaution Location

1. Labogene recommend wearing Biopharmaceutical &
safety glasses when connecting PharmacokineticsLab 2
flask or other equipment to freeze [ 05 C02 009/0 ]
dryer due to risk of implosion.
The freeze drying process is a
2. It is important that free air balance between the heat
circulation is available at the air absorbed by the sample to
inlet and outlet grills (there must vaporize the ice, and the heat
be at least 100mm free space at the removed by the condenser to
rear of the unit). convert the water vapor into ice.

PROCEDURE Sample must be frozen
for at least overnight (in
1. Switch on all plug points.
2. Switch on the vacuum pump. -80 freezer)
3. Mount the chamber and shelf arrangement on the freeze
Valve Valve The efficiency of the freeze
dryer, check all connections are tight and chamber lid is drying process depends
closed (make sure check the ice condenser is free from upward downward upon:
ice/water and that all valves i.e the drain valve is closed
and the chamber rubber valves in upward position.)  The surface area and
4. Switch on the Freeze Dryer by pressing the green button the thickness of the
*Wait about 30 minutes to allow the condenser to reach sample.
operational temperature (below -90°C).
5. Open the chamber and place the samples on the shelves.  The temperature of the
Close the chamber properly. condenser and the
6. Slowly turn the vacuum pump valve upward. vacuum obtained.
7. The freeze drying process is now in progress.
Sample Unload  The eutectic point
1. To stop freeze drying process, turn the vacuum pump (melting temperature)
valve downward. *[Do not switch off the pump yet. Let and the concentration
the pump run for a minimum of 45 minutes after the of the sample solution.
freeze-drying has ended. This is to avoid any possible
condensates remaining in the pump oil.] Freeze Dryer
2. Next, turn slowly one of the chamber rubber valve Temperature
downward to release the vacuum.
3. Remove all samples from the shelves. -110 °C
4. Switch off the freeze dryer (press the green button).
5. The freeze dryer can be de-iced by leaving the ice
condenser open at room temperature, also can pour few
amount of water and leaving the drain valve open.
6. Clean the chamber, shelves and all the accessories.
7. Switch off all plug points.