In Vitro Systems for Cell Culture - Greiner Bio One

In Vitro Systems miniPERM p. 2
for Cell Culture
lumoxTM dish p. 8
www.gbo.com/bioscience
quadriPERM p. 10
flexiPERM p. 11

lumoxTM multiwell p. 12

miniPERM

miniPERM Lab Scale Protein Production

A step forward in cell culture Applications
miniPERM is an economical easy-to use bioreactor which simplifies miniPERM is appropriate for use in a wide spectrum of research and
high density cultivation of hybridoma and other cell types. The main laboratory applications. In addition to cultivation of hybridoma for
focus is laid on cost efficient production of antibodies and other antibody production and transfected cells for protein production,
desired extracellular products. miniPERM is also applicable for virus production and general
proliferation of biomass.
miniPERM is suitable for cultivation of a variety of cell types. Depending on the cells used, cell densities up to 108 cells/ml and
High densities of both suspension cells and anchorage dependent cells product concentrations of several mg/ml can be achieved.
can be cultivated in the miniPERM. miniPERM is an effective alternative Special adaptation of culture techniques to miniPERM is not necessary.
to static culture, roller bottles and fermentor systems because high miniPERM is suitable for serum-containing and serum-free culture.
concentrations of cell products can be obtained even in small volumes.

Construction and Principle

miniPERM construction The system
miniPERM is a two-compartment bioreactor. The production module The miniPERM is available in two versions:
(with culture volumes of 35 ml) is separated from the nutrient module s The assembled miniPERM bioreactor, sterile:
(400 ml working volume) by a dialysis membrane (Fig. 1).
The production and nutrient modules are delivered assembled
Basic principle and sterilized. This represents a single-use product.
When the production and nutrient modules are assembled, s The reusable miniPERM:
the semipermeable dialysis membrane forms the dividing wall between The nutrient module can be autoclaved and reused.
the two modules. Neither the cells, nor high molecular weight products The production module is sterile and can be used only once.
released by the cells are able to pass through the membrane (Fig. 2).
However, nutrients and dissolved gases can diffuse from the nutrient nutrient module
module into the production module.

nutrient module
production module

production module

glucose Figure 1
lactate
Cultivation
NH+ In the miniPERM the nutrient medium as well as the cell suspension
must be rotated constantly, preferably on the Universal Turning Device
4 (Fig. 3). Then cells are optimally supplied and their metabolic waste
products are removed. The Universal Turning Device provided for use
CO2 cell dialysis membrane with miniPERM enables up to four bioreactors to be simultaneously
O2 cell product rotated. In order to achieve optimal gas-exchange, the miniPERM
bioreactor is rotated on the Universal Turning Device inside a CO2
silicone membrane incubator with a relative humidity of at least 70 %.

Figure 2: Principle of miniPERM

At the same time, low molecular weight metabolites secreted by the
cells can leave the production module via the dialysis membrane where
they are diluted in the medium of the nutrient module. The nutrient
module offers a large pH buffering capacity.
The outer side of the production module is sealed with a thin, gas-
permeable silicone membrane for an optimal gas exchange (O2 and
CO2).
The screw cap of the nutrient module with an integrated membrane
enables equilibration of pressure between the bioreactor and the outer
environment.

Figure 3: Universal Turning Device

IVSS 2 www.gbo.com/bioscience

miniPERM

miniPERM - Lab Scale Protein Production

miniPERM
Lab Scale Protein Production

Advantages Applications
s High cell densities s Production of proteins/
s High product concentrations
s Continuous harvest monoclonal antibodies
s Long term cultivation s Hybridoma
s Ease of use s Mammalian cells
s Wide application spectrum s Production of biomass
s Transfected cells
s Production of viruses
s Insect cells
s Plant cells
s Microorganisms

Selecting the Production Module

s miniPERM classic module medium. In applications for the proliferation of biomass, the
Research and lab-scale stationary matrix enables a simple biomass harvesting at the end
The miniPERM classic production module has a culture volume of a run as the cells are retained upon the matrix ( Fig. 1).
of 35 ml which is ideal for use in research and lab-scale
applications. Suspended cells or adherent cells on microcarriers
can be cultivated in the miniPERM classic. Whilst the miniPERM
is optimized for the cultivation of hybridoma cells, many other
cell types (e.g. CHO, BHK, SF, tumour cells etc.) can be
successfully cultured at high densities as well.

s miniPERM SM Figure 1
Stationary matrix for adherent cells
The SM production module is ideal for the cultivation of anchorage
dependent cells (e.g. HEK or COS cells). These modules contain
two polyester matrices upon which many cell types attach and
divide. The SM production modules have a growth surface area of
240 cm2. Cell products are normally released into the production

Cat.No. 9600 1059 9607 7009 9600 1055
Description miniPERM classic Kit miniPERM classic Test Kit Production Module classic
NMWCO* of Membrane [kD] 12.5 12.5 12.5
Sterile + + +
Quantity per case 12 miniPERM bioreactors 4 miniPERM bioreactors 12
1 Start-up Support Kit

Cat.No. 9607 7618 9607 7609 9607 7616
Description miniPERM SM 2105 Kit miniPERM SM 2105 Test Kit Production Module SM 2105
NMWCO* of Membrane [kD] 12.5 12.5 12.5
Sterile + + +
Quantity per case 12 miniPERM bioreactors 4 miniPERM bioreactors 12
1 Start-up Support Kit

* nominal molecular weight cut-offs

www.gbo.com/bioscience IVSS 3

miniPERM
miniPERM Accessories

9607 7041

9607 7320

9600 1054

9607 7137

9607 7134

9607 7135

9607 7136

9607 7138

The following accessories are offered for convenient handling during cellPROTECT
cultivation: cellPROTECT increases the viscosity of the culture medium and thereby
protects the cells against shear stress. cellPROTECT is added to the
Sterile single-use 50 ml Luer lock and 2 ml Luer syringes cell suspension in the production module at concentrations of 0.05 to
For simple and safe cell suspension inoculation, sampling and harvesting. 0.1% w/w.

Sterile filling tube 5'' antiFOAM a
The sterile filling tube offers a simple solution to cell suspension transfer When serum-containing medium is used, significant quantities of foam
(e.g. from centrifuge tubes to single-use syringe). The filling tube is may develop in both modules of the miniPERM. By adding 0.5 to 1 ml
slightly flexible, connects directly to a 50 ml Luer syringe and has a antiFOAM a to the nutrient module medium, this foam build-up is reduced.
sufficiently large inner diameter to minimize shear-stress induced cell
damage. Start-up Support Kit
Accessories to inoculate, sample and harvest. Consists of:
Luer syringe needles (25Gx 5/8'') s Filling tube 5", Luer, sterile (8x)
For sampling and supplementing the medium in the production module s Disposable hypodermic syringe 50 ml Luer, sterile (8x)
via septum ports. s Disposable hypodermic syringe 2 ml Luer, sterile (20x)
s Luer syringe needles (25G x 5/8"), sterile (20x)
Septum ports s Septum ports, sterile (6x)
To simplify sampling and supplementing procedures and to further s miniPERM stand (1x)
reduce contamination risks, a replacement of two of the three standard s antiFOAM a, 5 ml, sterile (1x)
screw caps on the production module with sterile septum ports is s cellPROTECT, 1ml, sterile (1x)
possible.
The rubber septum ports offer the possibility for addition of fluids to or
removal of samples from the production module using sterile single-use
syringe needles of small diameter. There is no need to open the module.
The septum ports should be replaced after they have been pierced
up to 10 times.

miniPERM stand
For mounting the miniPERM bioreactor during inoculation, sampling
and harvest.

IVSS 4 www.gbo.com/bioscience

miniPERM

Cat.No. 9600 1153 9600 1057 9607 7037 9600 1038 9600 1036
Description Nutrient module Screw cap for Screw cap for
nutrient module nutrient module Screw cap for Screw cap for
Sterile - - +
Autoclavable + + - production module production module
Quantity per case 4 30 16
-+
Cat.No.
Description +-
Sterile
Quantity per case 12 6

Cat.No. 9600 1054 9607 7134 9607 7138
Description Stand for miniPERM Septum ports Filling tube 5'' (127mm) Luer
- + +
Sterile 4 100 100
Quantity per case
9607 7136 9607 7137 9607 7135
Cat.No. Disposable hypodermic Disposable hypodermic Luer syringe needles (25 G x 5/8'')
Description syringe 2 ml Luer syringe 50 ml Luer Lock
Volume [ml] + + +
Sterile 100 60 100
Quantity per case
9600 1094 9607 7320 9607 7041
miniPERM Start-up Support Kit antiFOAM a cellPROTECT
- 100 100
+ +
1 1 1

www.gbo.com/bioscience IVSS 5

miniPERM Instruments for Cell Culture
Instruments for Cell Culture
Universal Turning Device Rocking Device
9600 1061
Advantages Advantages
s Flexible application s Simple set-up
s Space saving s Different angles
s Incremental rotational
of inclination
speed control
s Simple installation Applications
s Static cell culture vessels
Applications
s miniPERM bioreactors (e.g. quadriPERM, T-Flasks,
s Roller bottles petriPERM, petri dishes etc.)
s Centrifuge tubes s Protein-/DNA-staining
s Reagent vessels

Universal Turning Device The Rocking Device

A turning device for universal application Rocking Device
In contrast to standard turning devices, the speed of this Universal The Universal Turning Device can be adapted to a Rocking Device
Turning Device is digitally regulated across a rotational speed by using a modification set. This is a simple operation requiring
range of 0.1 to 40 rpm in steps of 0.1 rpm. It is therefore suitable few tools.
for most laboratory applications. Various static cell culture vessels can be mounted on the Rocking
The Universal Turning Device can be used with almost all laboratory Device which provides a work surface of 30 cm x 23 cm. Via the
incubators. The control unit remains outside the incubator and is adapter, two different angles of inclination (3° and 5°) can be set.
connected to the Universal Turning Device by a flat cable that The rocking frequency can be regulated between 0.1 and 40 Hz.
passes through the door seal.
Flexibility is guaranteed because the distance between the rollers 9600 1073
is variable. It is therefore possible to simultaneously rotate different
types of devices, e.g. roller bottles on one side and centrifuge
tubes on the other.

Special applications
The Universal Turning Device is ideal for high density cell culture
in the miniPERM bioreactor because the rotational speed can be
accurately regulated over a wide range. Optimal conditions for
supplying the cells and removing their metabolic waste products
and optimal gas exchange can be achieved.

Cat.No. 9600 1061 9600 1073
Description Universal Turning Device Rocking Device
External dimensions [cm] 45x10x25 45x10x25
Weight [kg] 7 7
Rated voltage [V] 240/120 240/120
Rated fequency [Hz] 50-60/60 50-60/60
Turning speed [rpm] 0.1-40 -
Quantity per case 1 1

IVSS 6 www.gbo.com/bioscience

miniPERM

Accessories for the Universal Turning Device

Accessories
for the Universal Turning Device

Enhanced flexibility
Special adapters are available for the use of centrifuge tubes and
reagent vessels in conjunction with the Universal Turning Device.
Three versions of adapter are available; one for up to 5 centrifuge
tubes or reagent vessels with volumes up to 50 ml, one for
12 devices with volumes up to 15 ml and one with a capacity to
rotate 48 units of 1.5 ml/2 ml tubes.

Cat.No. 9607 7360 9607 7361 9607 7362
Description Adapter for centrifuge tubes, Adapter for centrifuge tubes, Adapter for reagent vessels,
12 x 15 ml 5 x 50 ml 48 x 1.5/2.0 ml
Quantity per case 1 1 1

Spare parts for Universal Turning Device

Cat.No. 9600 1064 9605 5593 9605 5594 9629 0560 9605 5440 9629 1061
Description Motor Drive roller Slave roller Spacer Tray Control unit
240/120 V / 50/60 Hz
Quantity per case 11121 1

www.gbo.com/bioscience IVSS 7

lumoxTM dish

lumoxTM dish - Cell Culture Dish
with Gas-Permeable Base

Cell culture dish with gas-permeable base The lumoxTM dish principle
lumoxTM dish is a cell culture dish with a gas-permeable base made of
a 25 µm thick lumoxTM film (Fig. 1, 2). CO2
The cells grow directly on the border between gas and liquid phase,
where the culture medium does not act as a diffusion barrier. Therefore O2
an optimal transfer of CO2 and O2 is guaranteed. Figure 1: Gas exchange in lumoxTM dish is achieved via the lumoxTM film base.

Cells simply grow better! CO2
The gas permeability of lumoxTM dish is an enormous advantage.
Cells are efficiently supplied with O2 and excess CO2 is effectively
removed. The extremely short diffusion path ensures optimal gas
exchange. lumoxTM dish is available with a diameter of
50 and 35 mm.

Optimal growth conditions
The user can choose between a hydrophilic or a hydrophobic culture
surface. Therefore, both adherent (hydrophilic surface) and suspension
cells (hydrophobic surface) can be cultivated on lumoxTM dish.

lumoxTM dish TC O2
For very sensitive cells we offer lumoxTM dish (with ø 50 mm) with a
special tissue culture treatment which guarantees extra high adherence Figure 2: Gas exchange via the culture surface is not possible in conventional
for cells. petri dishes or culture flasks.

Applications in lumoxTM dish Electron microscopy
lumoxTM dish is routinely used in electron microscopy applications (Fig. 5).
Flexible base One advantage is that the gaspermeable film base can be easily cut
The film base of lumoxTM dish can be cut using a scalpel (Fig. 3). into ultra thin sections. Therefore it is not neccessary to enzymatically
Then the sample can be prepared for further applications, e.g. detach the cells before doing further experiments!
s Electron microscopy
s Inverse microscopy
s Phase-contrast microscopy
s Fluorescence microscopy
s Confocal microscopy

Figure 3: Cutting the lumoxTM film. Figure 5: Transmission electron microscopy image of a kitney epithelium cell
grown in lumoxTM dish hydrophilic.

The combination Growth of dendritic cells
The use of flexiPERM disc, a multipleuse silicone insert, divides lumoxTM Differentiation of human monocytes to dendritic cells within seven days
dish into four cell culture areas and thus allows parallel studies of a under the influence of cytokines (Fig. 6). At the end of the culture
single culture or transfection studies (Fig. 4). period, the cells could, without stress, be removed from lumoxTM film
after a short cold temperature phase. This is not the case with normal
cell-scraping or enzymatic methods.

Figure 4: flexiPERM disc divides lumoxTM dish into four smaller cell culture areas. Figure 6
lumox is a trademark of In Vitro Systems & Services, Germany

IVSS 8 www.gbo.com/bioscience

lumoxTM dish

lumoxTM dish - Cell Culture Dish
with Gas-Permeable Base

Advantages Applications
s Gas-permeable s Macrophages
s Crystal-clear s Stem cells
s Non-autofluorescent s Cardiac muscle cells
s UV-transparent s Skin transplants
s Chemically inert s Hepatocytes
s Microscope friendly s Dendritic cells
s Mycoplasma tests

lumoxTM Technology p. IVSS I 12

Cat.No. 9607 7331 9607 7333
Description lumoxTM dish 35 lumoxTM dish 35
Surface quality hydrophilic hydrophobic for suspension
for adherent cells culture cells
Volume per chamber [ml] 2 - 3.5 2 - 3.5
ø [mm] x height [mm] 35 x 6 35 x 6
Growth area [cm2] 9.6 9.6
Bottom lumoxTM film lumoxTM film
Sterile + +
Quantity per bag/case 5/50 5/50

Cat.No. 9607 7303 9607 7301 9607 7306 9607 7305 9607 7410 9607 7440
Description
Surface quality lumoxTM dish 50 lumoxTM dish 50 lumoxTM dish 50 lumoxTM dish 50 lumoxTM dish 50 lumoxTM dish 50

hydrophilic for adherent cells hydrophobic for suspension hydrophilic and TC treated

culture cells for sensitive adherent cells

Volume per chamber [ml] 5 - 10 5 - 10 5 - 10 5 - 10 5 - 10 5 - 10
50 x 12
ø [mm] x height [mm] 50 x 12 19.6 50 x 12 50 x 12 50 x 12 50 x 12
lumoxTM film
Growth area [cm2] 19.6 + 19.6 19.6 19.6 19.6
5/200
Bottom lumoxTM film lumoxTM film lumoxTM film lumoxTM film lumoxTM film

Sterile + ++ ++

Quantity per bag/case 5/50 5/200 5/50 5/50 5/200

lumox is a trademark of In Vitro Systems & Services, Germany

www.gbo.com/bioscience IVSS 9

quadriPERM

quadriPERM

quadriPERM - Cell Culture
Vessel for Parallel Testing

Advantages Applications
s Parallel testing s In-situ preparation
s Simple handling
s Use of standard of amniotic cells
s Chromosome analysis
microscope slides s Micronucleus assay
s Suitable for centrifuges s Replication studies
s Cytoskeleton staining
s Immunfluorescence tests

(FISH)
s Mycoplasma tests

Cell culture vessel for parallel investigations Application
The rectangular quadriPERM cell culture vessel with its four quadriPERM is particularly suited to routine pre-natal diagnosis,
subdivisions, offers space for slides with universally recognised e.g. for in-situ preparation of amniotic cells for chromosome
dimensions (26 x 76 mm). Cells can be grown simultaneously analysis. Further applications are found in in-situ preparation of
in the four separate compartments under identical conditions, chromosomes for cytogenetic testing, e.g. replication studies,
either on coated or uncoated slides or without slides for immunofluorescence tests (FISH), cytoskeleton staining or the
suspension culture cells. analysis of cell cultures using mycoplasma tests. quadriPERM can
also be centrifuged when used in conjunction with a microtitre
Easy-to-use plate adapter.
quadriPERM is very easy to use. Cells cultivated in quadriPERM
can be supplied quickly with fresh medium and directly examined The combination
under a microscope. Cells can be fixed, stained and immuno The individual culture compartments of the quadriPERM can be
histologically tagged. Cells grown on slides in quadriPERM can subdivided into smaller parallel culture compartments.
be used for all molecular biological and immunological This is achieved by the silicone culture inserts of flexiPERM slide
detection methods. flexiPERM micro12.

Cat.No. 9607 7308 9607 7307
Description quadriPERM quadriPERM
No. Chambers 4 4
Volume per chamber [ml] 10 - 15 10 - 15
Length [mm] x width [mm] per chamber 82 x 30 82 x 30
Height [mm] 15 15
Growth area [cm2] 24.6 24.6
Material polystyrene polystyrene
Sterile + +
Quantity per bag/case 12/192 12/48

IVSS 10 www.gbo.com/bioscience

flexiPERM

flexiPERM

9003 2039 flexiPERM -
9001 1436 Reusable Cell Culture Chamber

9003 4067 Advantages Applications
s Reusable s Nerve cells
9607 7435 s Highly adhesive s Endothelic cells
9607 7434 s For standard microscope s Tumour cells
s Toxicity tests
slides and petri dishes s Co-cultivation
s Non-cytotoxic s Transfection studies
s Slides to all smooth s Parallel testing

surfaces without glue
s Creates multiple growth

areas

flexiPERM - The flexible cell culture chamber flexiPERM disc
flexiPERM, a reusable cell culture insert made of silicone, can be flexiPERM disc splits every 50 mm diameter petri dish
combined with standard microscope slides (26 x 76 mm) and petri (e.g. lumoxTM dish 50 with a gas-permeable base), into four separate
dishes, creating multiple cell culture chambers. flexiPERM is heat cell culture chambers (Fig. 2). In addition to parallel studies with
and cold resistant, hydrophobic and non-toxic to cells. Because of one culture, flexiPERM can also be used for co-culture and
its highly adhesive underside, it sticks to all smooth surfaces, e.g. transfection studies.
glass, plastic and foil.

flexiPERM slide / flexiPERM micro12
flexiPERM slide with 8 and flexiPERM micro12 with
12 subdivisions, are perfect for parallel cell culture studies on
DIN standard microscope slides (Fig. 1). After removing the
flexiPERM, hydrophobicity remains in the zones surrounding each
of the culture areas. Individual staining of the different culture areas
on one slide is therefore easier to perform.

Figure 2

flexiPERM conA and conB
The flexiPERM conA and conB have been specially developed for
animal and plant physiology applications. Due to the cone shaped
form, cells grown on microscope slides in flexiPERM conA and
conB are suitable for applications in the field of micro-manipulation.
Cell growth can be examined under microscope and inter- and
intracellular changes can be quantified.

Figure 1

Cat.No. 9003 2039 9001 1436 9003 4067 9607 7434 9607 7435
Description flexiPERM flexiPERM flexiPERM flexiPERM flexiPERM
slide micro12 disc conA conB
No. chambers 8 12 4 1 1
Volume per chamber [ml] 0.88 0.35 1.6 2.7 4.6
ø per chamber [mm] 11 x 8 5 15 12 20
Height [mm] 9 9 9 8 8
Growth area [cm2] 0.9 0.2 1.8 1.0 3.1
Material silicone silicone silicone silicone silicone
Reusable + + + + +
Quantity per case 5 5 5 5 5

www.gbo.com/bioscience IVSS 11

The New lumoxTM Technology

The New lumoxTM Technology

lumoxTM technology The principle of the lumoxTM technology
The new lumoxTM technology offers cell culture systems based on an opti- The lumoxTM film base is permeable for gases, e.g. O2 and CO2. By this
cally clear gas-permeable film. The lumoxTM multiwell plate consists of a means, optimal growth conditions and therefore homogeneous cell
black polystyrene body the external dimensions are conforming to the growth can be achieved (Fig. 2).
recommendations of the Society for Biomolecular Screening (SBS).
Depending on the cell types, a choice of lumoxTM surfaces ensures the
The transparent base of lumoxTM multiwell is made of an ultra thin (50 µm) ideal cultivation environment.
gas-permeable film with very low autofluorescence. The particular
specification of the film results in excellent optical features making the
lumoxTM multiwell plates suitable for microscopy, in particular for
fluorescence microscopy.

Figure 2

Figure 1

Applications

The range of applications for lumoxTM multiwell, from cell culture to Tips and Tricks
lumoxTM film is thinner than cover glass. Therefore we suggest the
automated analysis of fluorescent cell-based assays for drug screening: following:
Conventional light microscopy: If cover glass corrected objectives
s Cell assays s Fluorescence microscopy with a focus above 1mm are used, it might be necessary to use
cover glass caps to achieve maximum image quality.
s Drug Discovery s Phase contrast microscopy
Laser Scanning Microscopy/ Confocal Microscopy:
s High-Throughput Screening s FCS For best performance, the use of non-cover glass objectives is
recommended (focus 0 - ∞).
s Sub-cloning s LSCM
Phase contrast microscopy/ Varel contrast microscopy/
s Cryopreservation s FISH Hoffmann contrast microscopy:
The applications work perfectly with lumoxTM if an objective lens with
s ADME / Tox s FRET a focus of 0 - ∞ is used. Otherwise it is necessary to utilise a cover
glass cap to achieve excellent results.
Cell culture data
A stable cell line of PTK2 cells expressing beta-tubulin construct Oil immersion: Application of oil immersion is not recommended.
(see reference: Nat Cell Biol 2001 Feb; 3(2):140-9) was cultivated and
visualized in lumoxTM multiwell plates, with resultant excellent image Mounting media: Fixation with standard mounting media can be easily
quality. performed (see table 1 p. IVSS l 14 for chemical compatibility).

lumox is a trademark of In Vitro Systems & Services, Germany
IVSS 12 www.gbo.com/bioscience

Technical Information The New lumoxTM Technology

1. Optical data lumoxTM multiwell plate, tissue culture quality

To evaluate autofluorescence, emission spectra were investigated in
empty multiwell plates. lumoxTM multiwell plates show a very low
autofluorescence independent from chosen excitation wave length
resulting in a unique suitability for fluorescence applications (Fig. 2).
Measured background signal was 10 times lower in lumoxTM multiwell
plate compared to polystyrene plates (Fig. 1).

lumoxTM multiwell vs polystyrene

lumoxTM multiwell vs glass Figure 2: Emmission spectra with fixed excitation, lumoxTM multiwell plates,
tissue culture quality vs polystyrene multiwell plate

2. Growth Curves

Cells were seeded in different concentrations in the lumoxTM multiwell
plate. Independent from cell type and concentration, cells grow much
faster in lumoxTM multiwell in comparison to the given reference data
(Fig. 3, 4).

Figure 1: Transmission of lumoxTM multiwell compared to glass and Figure 3: CHO cells in lumoxTM multiwell, tissue culture quality
polystyrene plates

Polystyrene multiwell plate

lumox is a trademark of In Vitro Systems & Services, Germany Figure 4: BHK cells in lumoxTM multiwell plates, tissue culture quality
www.gbo.com/bioscience IVSS 13

The New lumoxTM Technology

Solvent Temperature Time of Exposure Conc. Result
DMSO Room temperature
Ethanol Room temperature 24 h and 72 h 10 %
and 37 ºC
Methanol Room temperature 24 h and 72 h 70 %
and 37 ºC
Formaldehyde Room temperature 24 h and 72 h 70 %
Glutaraldehyde Room temperature
Paraformaldehyde Room temperature 24 h and 72 h 50 %
Acetone Room temperature
24 h and 72 h 50 %

24 h and 72 h 4%

24 h and 72 h 5%

24 h 4 %

15 min. 100 %

(Polystyrene of the plate body affected, membrane slightly arched in)

Tris HCl 37 ºC 24 h and 72 h 100 %
Tween 20 37 ºC
Trypsin 37 ºC 24 h and 72 h 20 %
EDTA-Trypsin 37 ºC
Triton X 100 Room temperature 24 h and 72 h 0.25 %
and 37 ºC
Distilled Water 37 ºC 24 h and 72 h 0.03/0.25 %
PBS 37 ºC
DMEM 37 ºC 24 h and 72 h 20 %
RPMI 37 ºC
Immersion Oil Room temperature 24 h and 72 h 20 %
Room temperature
Glycerol Room temperature 3 weeks 100 %
Table 1: Solvent Compatibility
3 weeks 100 %

3 weeks 100 %

3 weeks 100 %

3 weeks 10 %

3 weeks 100 %

3 weeks 50 %

IVSS 14 www.gbo.com/bioscience

lumoxTM multiwell The New lumoxTM Technology
lumoxTM multiwell
Formats
lumoxTM multiwell plates are available as: Advantages s Optimal growth conditions
s 24 well format s Very low autofluorescence s Homogenous cell growth
s 96 well format s Optical clarity s Different growth surfaces
s 384 well format s Suitable for microscopy s Excellent temperature transfer
All lumoxTM multiwell plates have TC surface for s Gas-permeable base
ultra sensitive cells. s Optimal oxygen supply /

CO2 removal

Cat.No. 9611 0024 9600 00141) 9612 0096 9600 00241) 9613 0384 9600 00341)
Well format 96 well 384 well 384 well
Well profile 24 well 24 well 96 well F-bottom/ F-bottom F-bottom
chimney well
Bottom F-bottom/ lumoxTM film lumoxTM film lumoxTM film
Growth area per well [cm2] 0.34 0.11 0.11
Working volume per well [µl] chimney well 25 - 340 10 - 130 10 - 130
Lid +2) + +
TC surface treatment lumoxTM film lumoxTM film lumoxTM film + + +
Sterile + + +
Quantity per bag/case 1.9 1.9 0.34 1/4 1/20 1/4

5000 - 15000 5000 - 15000 25 - 340

+2) +2) +2)

+++

+++

1/20 1/4 1/20

1) Trial Kit 2) with condensation rings

lumox is a trademark of In Vitro Systems & Services, Germany

www.gbo.com/bioscience IVSS 15

For further information, please visit our website www.gbo.com/bioscience or contact us:

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