1°-6° C 20°-24° C ≤ -18° C AABB TEMPERATURE STANDARDS BLOOD PRODUCTS BY TEMPERATURE GROUP: STORAGE AND TRANSPORT (AABB)
1°-6° C 20°-24° C ≤ -18° C AABB TEMPERATURE STANDARDS BLOOD PRODUCTS BY TEMPERATURE GROUP: STORAGE AND TRANSPORT (AABB)
Component Storage Transport Whole Blood 1°-6° C 1°-10° C Whole Blood, Irradiated RBCs (Red Blood Cells) RBCs, Deglycerolized RBCs, Irradiated RBCs, Leukocytes Reduced RBCs, Rejuvenated RBCs, Deglycerolized Rejuvenated RBCs, Washed RBCs, Apheresis RBCs, Apheresis Leukocytes Reduced Plasma, Fresh Frozen Plasma FFP (after thawing) Plasma, Frozen Within 24 Hours After Phlebotomy (after thawing) Plasma, Thawed Plasma, Cryoprecipitate Reduced (after thawing) Plasma, Liquid AABB TEMPERATURE STANDARDS BLOOD PRODUCTS BY TEMPERATURE GROUP: STORAGE AND TRANSPORT (AABB)
Component Storage Translport Platelets 20°-24° C Continuous Gentle Agitation 20°-24° C Platelets, Irradiated Platelets, Leukocytes Reduced Platelets, Pooled Leukocytes Reduced Platelets, Pooled (or open system) Platelets, Apheresis Platelets, Apheresis Irradiated Platelets, Apheresis Leukocytes Reduced Apheresis Granulocytes Apheresis Granulocytes Irradiated AHF, Cryoprecipitated AHF, Thawed Cryprecipitated AHF, Plasma Thawed Cryoprecipitated
Component Storage Translport AHF, Plasma Cryoprecipated ≤ -18° C MAINTAIN FROZEN STATE AHF Pooled Cryoprecipitate d (before freezing) Plasma, Frozen Within 24 Hours After Phlebotomy Plasma, Cryoprecipitate Reduced FFP – Plasma, Fresh Frozen ≤ -18° C OR ≤ - 65° C ≤ -18° C OR ≤ - 65° C Reference: “AABB Standards for Blood Banks and Transfusion Services,” 32nd Edition – Effective July 1, 2020
S. No. PRODUCT STORED IN TEMPERATURE EXPIRATION 1. Whole Blood Refrigerator 1-6 °C 35-Days 2. Packed Red Blood Cell Refrigerator 1-6 °C 35-Days* Open systems: 24 hours (e.g. washedrbc’s) 3. Fresh-Frozen Plasma (FFP) Freezer -18 °C or below 12-months 4. FFP-Thawed Refrigerator 1-6 °C 24 hours 5. Platelets and/or Apheresis units (a) Platelet incubator with agitation(b) Platelet incubator without agitation 20-24 °C 5-Days 24 hours 6. Cryoprecipitated Anti-hemophilic factor (AHF) Freezer -18 °C or below 12 months 7. Cryoprecipitated AHF (Thawed) Room Temperature (20- 24°C) 20-24°C (a) 4-hours open system(b) 6-hours closed system STORAGE CONDITIONS OF BLOOD PRODUCTS Anticoagulant = CPDA-1
CDC Recommends Digital Data Logger or DDL A DDL should have the following features: •A ‘buffered temperature probe’ as they measure the actual vaccine temperature. thermometers will only measure the temperature of the air. •alarm for out-of-range temperatures •Low-battery indicator •Current, min and max temperature display •An accuracy reading of +/-0 .5°C (+/-1° F) •Logging interval (or reading rate) that you can program to measure and record temperatures at least every 30 minutes •Current and valid Certificate of Calibration Testing
Best Practices Guide for Selecting Blood Bank Refrigerators Storage devices shall have the capacity and design to ensure that the proper temperature is maintained. (AABB Standard 3.6.1) Whole blood and red blood cell components should be stored from 1ºC to 6ºC (AABB Reference Standard 5.1.8A). ▢ The refrigerator is designed to support a set point of 4ºC ▢ The refrigerator is designed with a heavy-duty, forced-air refrigeration system ▢ The temperature uniformity of the refrigerator is +/-1ºC Storage temperatures of refrigerators, freezers, and platelet incubators shall be monitored. (AABB Standard 3.6.2) ▢ The refrigerator"s controller monitors the temperature inside the cabinet and high or low temperature alarms activate if the temperature exceeds or falls below the alarm limits ▢ Other factors that could impact the temperature, such as door openings and power failure, should also be monitored with alarm For storage of blood or blood components, the temperature shall be monitored continuously and recorded at least every 4 hours. (AABB Standard 5.1.8.1.3) ▢ The refrigerator's controller constantly monitors the temperature inside the cabinet ▢ The refrigerator has a chart recorder that records the temperature inside the cabinet ▢ The refrigerator should be designed with remote alarm contacts and a probe port to enable monitoring and recording with 3rd party systems Reference: AABB, Standards for Blood Banks and Transfusion Services, 30th Edition
Harmful effects of Improper Storage. If blood is stored or transported outside of these temperatures for long, it loses its ability to transport oxygen or carbon dioxide to and from tissues respectively upon transfusion. Other factors of serious concern are the risk of bacterial contamination if blood is exposed to warm temperatures. Conversely, blood exposed to temperatures below freezing may get hemolysed and can lead to a fatal transfusion reactio Safe storage of blood A. Whole blood : Whole blood and red cells must always be stored at a temperature between +2 °C and +6 °C. If blood is not stored at between +2 °C and +6 °C, its oxygencarrying ability is greatly reduced. The anticoagulant/preservative solution in the blood bag contains nutrients for the blood during storage and stops the blood from clotting. The red cells can carry and deliver oxygen only if they remain viable. C. Platelet concentrates Platelet-rich plasma (PRP)/ Platelet concentrate (PC) must be separated from whole blood by centrifugation within 6 hours of collection. whole blood should be kept at between +20 °C and +24 °C until it is processed into platelet concentrates and other blood components. Platelet concentrates should be stored at a temperature of between +20 °C and +24 °C i.e 22±2 °C with continuous gentle agitation. This is essential to prevent platelet aggregation which results in loss of viability
Length of Time Permitted For The Storage And Transportation of Platelet Concentrates Within The Temperature Range +20 °C To +24 °C