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ز ن و fl م د ffi ن ffl "!رfi ff #$ ٥ ١(٠٢&رد- | ر- , . دور / م، !1ی1 3 :Feline Infectious Peritonitis fl ffiffl ff fi ! " . #fl$ fl% % & '( fi # ,-$ . /ff 0- /fl *fl( +# ff -fl7 '( 6,2$ *" /12-fl34 /52 fl . 28# 9- ,#fl:. fi$fl1 ;< ff = -# 1#fl:. #fl$ : 628 . - 2:fl 0 ? & 08/>fl5 ff = -# @flA 4: 6@fl -flB flC /fl fi ;$ E1 .# ,-$ 1 -# 02fl1 D5flfl . #fl$ " '( 8 = -# 2 D5flfl fl fl2 F &G '( ffflH :Canine Babesiosis -# 0- - I -# H>flB -# J I #fl$ 8 #fl$. = *$ 3 -flB flC 12ff ?E :NeosporaCaninum fi 8 -# fiL ff B fi -# fiL ff 0# fiL ff :fl< / -# *M fiL ff 0# fiL ff NM .fi ,2$ $ /H -# 28 2. AP *$ 8fiL ff 0# 0- #M ffO NflC 8fiL ff ,#fl:. 0QR S. T7 *:2 UflA( N" .28 fiflA( 28# . 9- 8= fl ,2$ ;O# 08fi 6 <U 6 DZ(- 7( VWX 2fl = -# fffl2-flB flC 8 . 2 #I fl5 0fl- : 08 />fl5 [34 *5 /2 - \2M 0 0ff ,#. - ]" 4 8 fffl8 flA( *fl( fi 0- X *\ 2:fl *fl( fi?$ .2-# &Efl 08@# -# *\ 2:fl - -# -# - <\ 2:fl VA< 2fl ^<R fiflA( ff 01fl< 0 . 28# ]8 VflM : 1) Failure of Pregnancy in dogs and cats – Robert Foster , Clinic 2) al therlogenology 2022 : 14;67 3) Infectious causes of embryonic and fetal mortality – MD Givens ,marley MSD . theriogenology 2008 ; 270-285 4) Infectious Causes Of Abortion In The CatDonald H. Lein- 1998 5) Infertility in Toms- sylwiaProchowska and WojciechNizanski- Journal of Feline Medicine And Surgery 2022; 837-846 6) Canine Babeesiosis A disease rarely considered in the context of male infertility- Irish Veterinary Journal 2020; 73;72 7) Small Animal Internal Medicine- Richard W Nelson – Fifth edition 8) Johnson SD, Root Kostritz MV, infectious causes of infertility, abortion and stillbirths in cats – marrow DA, editor. Current therapy in theriogenology. 2nd edition, Philadelphia: W.B. Saunders Company ; 1986 p834 9) Smith KC .Herpesviral abortion in domestic animals. Vet J 1997;153;253-68 10) Anvik JO – clinical considerations of canine herpesvirus infection. Vet med 1991;86;394- 403 11) Carmichael LE ,schlafer DH ,hashimoto A. pathogenicity of minute virus of canines for the canine fetus . cornell vet 1991;81:151
ز ن و fl م د ffi ن ffl "!رfi ff #$ 2 دور+ 1/ی/، !م -, ر+ | رد%٠٢'١ 3 ... : 1) Freire DAC. Abortion and fetal death in bitches with infectious anemia (Dissertation). Brazil: Universidade Federal Rural do Semi‐Árido; 2016. 49 p. 2) Givens MD, Marley MSD. Infectious causes of embryonic and fetal mortality. Theriogenology. 2008;70:270–285 3) Graham EM, Taylor DJ. Bacterial reproductive pathogens of cats and dogs. Veterinary Clinics Small Animals. 2012;42:561–582 4) Greene CE, Carmichael LE. Canine brucellosis. In: Greene CE, editor. Infectious Diseases of the Dog and Cat. WB Saunders Co. Rio de Janeiro, Brail; 2006. pp. 369–381. 5) Dubey JP. Toxoplasmosis in dogs. Canine Practice. 1985;12:7–28. ISSN: 1057–6622. 6) Dubey JP, Lindsay DS. TransplacentalNeosporacaninum infection in dogs. Am J Vet Res 1989;50:1578–9. 7) Greene CE, Carmichael LE. Canine herpesvirus infection. In: Greene CE, editor. Infectious diseases of the dog and cat. 3rd ed., St. Louis: Elsevier; 2006. p. 47–53. 8) Foley JE, Pedersen NC. The inheritance of susceptibility to feline infectious peritonitis in purebred catteries. Feline Pract 1996;24:14–22. 9) BulginMS,Ward AC, Sriranganathan N, Saras P. Abortion in the dog due to Campylobacter species. Am J Vet Res 1984;45: 555–6. flffifflff fi # $ flffifflff fi !" 01 « fi !" fl*"+(fl, -. % » % & ffi '(" 8 '" 7 $ 5 0, 6 # %-4 23 !" .%- 9 3 <$+ + " => & ? @ :ffl; $ flffifflff fi !" fl*"+(fl, -. ..# A flffifflff fi !" & ( D *% 0, 6 + C1 B !- $ (,+ + F5 GH$ fl. # E'" '.+K@ + ffi J # I3 #5 & 1 . + ( # .+K@ G" #!" 01 #%-' %" .GH$ . ( L fl. + E'" (9 . + # . (M" %-4 L 23 (,+ + F5 .%--4 8 ( word +) 9 0, 5 0, 6 (ffi & 1 <R9 , 0, 6 =Q D fi P W$ - 4X -V.*7 .%-ffi $ ( )10 S4%1 flffifflff + (Y W$ - (1 + D ffl, &%ffi B 0, 6 . ffi & ( , R . I3 2P R 8 + (' *$ 2 3> 0M4 $ %- 3 [email protected] fl*"+(fl, DM@ $ %-"L %-. . @*fi
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ز ن و fl م د ffi ن ffl "!رfi ff #$ 4 دور- 13ی1! ،م /. ر- | رد'٠٢)١%& 9-1- fl ffi fflff ffi fi ffi !" ffi +,$-. ( /0 1 *23 &' (ff !) !* .!! #$ffl # ff ffi2 8 -, ! .!! 7,/$.45, /,6 ff . 9: ( 1 * ; . !! / ff +,$-. 10-1- #@ @)ffi 70 #06 ffi $,3 15 & 25 1 *23 @ @ 1 @*23 A@ +2@ #06 /ff B C) .!! . !!@ ffiDE -* fiffi fi ! / # 2$ 8 @F@B G@ (fiffi 1 @*23 A&H2@ 8 (!@ F@" !@* 10 CFU/g *23 .!! ffi 3 # F'ff 5 ff @ @ $,3 30 1! 1 *23 !* -J .ffi ! +,$-. I ffi ffi @ @*23 3 ffi ,. .!! F" 56,fi0, . ! L I &fi0, & ffi C) 1 *23 &MB 11-1- !!@ 7,@/$. fi / fi 7 & MB C) 1 *23 @@ !@@3 L @@ff O@@fflff fi@@ ( @@ffl* Ofi fi@@ 1 .( Ofi!@@)) 2% A ( @,ff 7-,@L @ !@ MB Qfi fi . &MB 5/2 % fi @@J 2 % ( @@,ff 7-,@@L @@ !@@ M@@B Q@@ fi@@ 7-,L ! MB Qffi C' fi A , T (,/6 7Uffl@B fi@ A ,@ T (,/6 5 % fi J 2 % ( ,ff T @ (,/6 4 % fi J 2 % ( ,ff 7-,L ! MB 2 % ( @,ff 7-,@L @ !@ M@B 7@ fi@ AT,- ffi WE (,@@/6 4 % fi ,@@ @@ T @@ (,@@/6 5/2 % fi @@J 5 % fi @J 2 % ( @, ff 7-,@L @ @ff 7@X fi@ fi T,@- ffi @WE T,@- ffi @WE (,@/6 fi4 % ,@ @ T @ (,@/6 @fiffi ffi &fi0, & ffi C) ffi ,. TY . ! &MB @@@)ffi 4 @@@ ffi ffi !@@@C ffifi O@@@: 0 A4 A8 A12 fi16 ffi . !! ffi !C , Table 1 List of treatments in the present study. NO Treatment Description 1 Con Samples without any coating solution 2 CH Samples coated with Chitosan solution 3 CH+BPNE1 Samples coated with Chitosan solu%on containing 2.5% BPEO 4 CH+BPNE2 Samples coated with Chitosan solu%on containing 5% BPEO 5 CH+MONE Samples coated with Chitosan solu%on containing 4% MOE 6 CH+BPNE1+MONE Samples coated with Chitosan solu%on containing 2.5% BPEO and 4% MOE 7 CH+BPNE2+MONE Samples coated with Chitosan solu%on containing 5% BPEO and 4% MOE &fi0, 4,6 12-1- ffi @ @ @ Q 25 45, /,6 \ffi (5@ fi ^@-_ 1/0 % @YB 8 @,6&-, 225 # ] @ @,6 &@-, .1/0 !@ ,C. I ffi 3ffi ( `3 * fi ! . !@ @ff ffi @ &a W" ff ],H +2 fiffi 3ffi @@ff ],@@H 45,@@ /,6 ff @@ \ffi @@ @@C) ffi ff !* .! X (( 6 Ab) ffi Q 06 B &a W" ffi c@ @ " Q@ E @ 24 1!@ @ , )ffi 37 .! (TBARS) !, Fffi,,ffi ,. , ! 13-1- ffi !@@,d!6 (6 @@ Q@@&@@-, 1ffi@@W TBARS @@, !@@ @,6 &@-,90 @ Q .10 ! ( , Q-,ff O @$ @,2$. e6ffi #a J ^-_ .! ^-_ 2$ 8 f@ff !; O 4 Fffi!-ff !, ,6&-, ! .5/2 #@afi @,2$. @ @ e6ffi fi L ; A^-_ \) !; fi T@B ! ,2$. ,6 &-, 50 fi ! 1ffi J ^-_ ! . @,2$. @ @,6&-, .5 ! ffifig) ^-_ \) ( F,@ !,@ @,6&@-, )10 TBARS 9@* @,6&@-, 5 fi !@ ffi ffi @ @66 @ (TBARS ffi@B Q@ /0 2883 90 % fi O , h \@) 8 ffi @$,3 35 1!@ @ # ff ( ( 0. TB fi #$ffl .!! ffi 3
ز ن و fl م د ffi ن ffl "!رfi ff #$ 2! ، م 0 دور. -+ی+ / ر. | رد%٠٢'١ ٣٢ fl ffiffl fffi 35 % )$1 %#$ % fi& ' ( $ fffi 10 ffi . (. 538 * +, #ff × 8/7 % fi& = (120fi3 fi456 75 12)0fi )TBA pH %fi2( 14-1- ' 2 7@ Aff B6 fi5)0fi 90 ( 12 10 . %fi2( pH 7Efi +fiF pH C$D % 6 #fi0GD ' EFD 15-1- . (O$ SPSS16 EN1 M Efi56 KLfl H'I J JfiQfi & ff . 2 1F D H 6 ) D 3 p<0/05 ) ) D . 1F ANOVA 7 6 0fi$' . N2 T %)R ffl 7S 2- (fi$ ( $ ) fifi ffiVfi3D 2-1- % 2 )N2D '3 (Q$ C$D $ (#fiWD ffiVfi3D $ Zfi3D ' 25 2 )$ ) fl M$ Yfi, #X +'fl $ ( #fiWD ffiVfi3D 3 ) $ (fi$ ( ( #fiWD ffiVfi3D J L )'N +'fl . J K$ ( 6 2 . K$ ( 2 [\W ffiVfi3D J % $fl 7 ' $ fi56 Jfi 3 : DVS $ ( #fiWD )0] ffiVfi3D -7- + 7 % 7 )99/19 (% `1-4- Jfi D O56 ` )37/24 (% bc. dffl R5A M )71/9 (% . J $ ' )96/18 ((% . J KffA Q JfiffffG C$D ( 1F ffiR5A e KGD D) L6 (f ( 7Efi ' ) fi ffiVfi3D bc. K& `J$ ` 7 `) fiNgfl ffA #fiV )A . 3 fifigD %fi2 $ (G ' (fi2 (O$ )39/38(% ` fi56 J 3 `(2020) 7 ' %'&\fi3 ffiVfi3D J Wfi 7R )44/4 (% Jfi D 2 ' )23/5 (% J $ (2017) 7 ' )V5, ]28 .[ 3 7fi (fi$ ( $ #fi 2- + ' (fi$ ( $ (#fiWD ffiVfi3D J Wfi )37/12(% +Dfi ' )23/18 (% J $ ` )08/34(% #fiN 3- – Jfi D 2 `(2010) 7 ' 7fi0S *'S ]29 .[ 3 E2 -7- +)5/10 (% Jfi D 2 ' )6/19 (% fi56 Jfi 3 ` )2/44(% fiff ]30 .[ffl 3 )NR (fi$ ( $ ffiVfi3D J Wfi 7R (fi$ ( $ ffiVfi3D J Wfi `(1393)7 ' ffi&5 ' )28/17 (% fi56 J 3 ` )86/21(% Jfifi D 2 ]31.[ 3 7fi )21/6 (% Jfi$ Table 1 Composition of Buniumpersicumessential oil. NO Constituents Concentration (%) RT β 0.28 8.24 -Thujene 1 2 Cyclohexane, (1-methylethylidene)- 0.26 8.38 3 Camphene 0.18 9.16 β 0.49 10.07 -Phellandrene 4 β 5.28 10.28 -Pinene 5 β 0.52 10.73 -Myrcene 6 α 1.32 11.46 -Phellandrene 7 α 0.14 11.91 -Terpinene 8 9 p-Cymene 9.71 12.33 10 D-Limonene 6.29 12.49 11 Eucalyptol 0.48 12.62 0.10 12.75 trans-β-Ocimene 12 γ 19.99 13.84 -Terpinene 13 14 Terpinolene 0.50 14.98 15 4-Terpineol 0.31 19.49
ز ن و م د ffl ffi ن fl "!رfi ff #$ 3 دور, 02ی0! ،م .- ر, | رد&٠٢(١%% 16 Dill ether 0.13 19.76 α 0.14 20.20 -Terpineol 17 18 Estragole 0.24 20.37 19 Cuminaldehyde 24.37 22.62 20 Phellandral 0.18 24.07 21 Carbamodithioic acid, formyl-, methyl ester 8.75 24.56 22 1,4-p-Menthadien-7-al 18.96 24.82 23 Caryophyllene 0.16 30.07 24 Myristicine 0.12 34.47 25 Heptasiloxane, hexadecamethyl- 0.98 59.57 Total 99.88 MBC MIC 2-2- #$ff%&' fl ffifflfffi #$ff%%'" fl ffifflfffi ! & 3 (ff) * MBC MIC #. ! . ff, ** 25/0 % 5/0 % 3 4 4 0. 12! ! / !'. 0 fl ffifflfffi (2020) ;<= / : ' .ff* $ 91$ 0. #$ff%&' fl ffifflfffi #$ff%%'" /=0 /*ff@4 0 A,* B . ; 9 ffifflfffi Dff* ' # .12! ! ff ! !C< C< 0 #$ff%%'" fl 1 mg/ml 2 mg/ml 3 4 4 0.12! ! ff ! ! 0 #$ff%&' fl ffifflfffi 4 mg/ml 3 4 4 0.12! ! ff ! !C< ;<= J 0@K * ]28.[ ff* $ 91$ 2 mg/ml Aff%L 0 #$ff%%'" fl ffifflfffi D(2010) O . D OP O'' Q! 0<) #Mfl 24N ! ff ! ! C< DO157:H7#' & , DOP ! ff* $ 91$ 05/0-03/0 mg/ml 0%* *12! V $ /=/ !'. 0 #. ; ffR ST U . W.K 0@K * Rfi ! . 0' *. #X% V $ YZ MBC MIC #. ! **]30.[ . ff* $ 91$ 1 % 2 % 3 4 4 0.12! ! / !'. 0 #. ; ffR ST . 0K. * /0@K . 0!Q U [S %'4 $ A #' K. $ A #Lffl ffR #. ; ffR ST . [\W /*ff@4 #. ; ffR ( ] 2015) ;<= #<' .** \* ^* 9 0. OP O . / !'. 0 # ; /* $ A 0' ** & ! .ff* ' # . 2 X* ff,.]32[ . # OP . / !'. 0 #.T #. ; ffR ] Table 3 MIC and MBC of Buniumpersicumnanoemulsions (BPN) and MelissaofficinalisL. extract (MOE) against Listeriamonocytogenes by microdilution broth method. Nanoemulsion MIC (%) MBC(%) Buniumpersicum 0.25 0.50 MelissaofficinalisL. 1 2 &! 9 0. ff, 0 "4 /= _ Q #. ; ffR @Q 2-3- ^* /fi ! ' _ Q #. ; ffR ] *X! . #Mfl * #, # !'. 0$ * . . * $ 0' K<= . Q $ ffl `a * 2 X* ^* 9 1 /fi ! ' _ Q ff=*# & ! / !'. / . #. ; ffR ] A !& . 4 % 5% ! ' _ Q # !'. ffR ] A !<' 12! ! 0. \<!fi 0' ff%!,* 5/2 % /fi 2.( (ff) ) ff,.# ff =ffa A ' A %N 4 $ /\. *W ffi *
ز ن و fl م د ffi ffl "!رfi ff ن $# 2 دور+ 1/ی/، !م -, ر+ | رد%٠٢'١ ٣' fl ffi ffl ffl fffi " # "$ % & '% ( )ffl *+, # ! ffl 2!") *+1) ' ' fi /0 # # $ % & . - # # ffi 4% 2 *3 ) . ) ' ' fi /0 fl3" #" 3"7 6"2 . *" ' " 5" " % fl3" <2%": # ";) "fi 830% 9: *+1) ' ' fi /0 *) ?" ">% " ") /"0 ' ' fi fl3 # ) =* '% "( 830% "9: " %A" % " '"- 0 "ff: @ "fffi ") " . fi "9fl <2%": "fi % @B"3?) ' fi # ",3: " *" ># ffi C -D '# ) ' ' fi /0 fl3 # 6+" H") " "G) . ": # ") #" ># "ffi EF" /"0 ' "fi ' " " *+, ) ' ' fi /0 *)% ;) IJ I"J 0 "# "! ffl " & " *"K3D IJ *+1) ) . # " & " "fi " ffl 2% ) *K3D ' fi B3? ) 4L ;) *J . # *"K3D @& " N%# O2 @ 0 &> *3 M * P3"J "fi H8"-2 5 ) fffi % & % !) # 4 +2 " *"7 ffi '# ffl" U " T fi ]33 .[ ) ' "# W"fi EF P3J fi # ffl ' >% ]34.[ * %8) "- " "fl fi (& X J ) ) P3J ' fi @ T fi -) ffl" "%8) " *fi ) * M : *3 . " # " ")M ' "fi %"0 >% *3 * 2U 0Z% Y ffi# ) \J 4> O) % %8) [fl ! *+1) ) @ " P3"J EF" " ] "2 " # "fi' " "# % "O2 ffl 0 ]13.[ '03> / ""G (1389) # ""8fi % ""fl &F""2 "" ""\fl F) ' # ' "fi # ) fl fi / - - _ @*J[: 21 &3 ]3 @3) 45 FX ]a,3 `) 18 '",83JAT @3) 20]a#% % H J @3) . " @3) 8 /#2Afl % 3)163 - @3) @ ffl & cffi 37 Hfl /A OM ]35.[ * M # a flM ) #d( # "$ " " - (2015) # 8fi % fl # f Y # % ]a"",3 '%# "" "" "" ""! &""3) ""8fl ]32 .[ 3) 12 \fl F) # # ffl *\ ) FX fl fi - Table4 Antibacterial activity of chitosan films containing BPNEand MONE against Listeriamonocytogenes by the agar well diffiusion assay Treatment Listeria monocytogenes 11.30±0.17 g CH 13.46±0.18 f CH+BPNE1 15.66±0.18 e CH+BPNE2 14.30±0.15 d CH+MONE 16.47±0.14 c CH+BPNE1+MONE 17.15±0.16 b CH+BPNE+MONE 30.19±0.14a GM Different letters indicate a statistically significant difference (p <.05). P3J , M *fl \J ffl# fi 4-2- n" Ifi m DPPH 3l) # $ % & %J n"J *"Jr T q# *O> D . # ) o[> # 517 m"" fflM ' ""fi ""8 # . *"" # ""$ % & "" ""8 # . " " ") ["D ") # 5 +" D s ' fi ,2 " "- 1 H8" # " , M )fflM _ ] P3"J ",M *fl \J ffl & ) IJ @* ", "M *fl \J - '#f *J IJ #ffi 0 # $ 4 % % ffl & 5 % '% ( ffl P3J `) P3"J " `") ", "M *"7 ffi ) . &3) fl ffi ffl ffl fffi ffl & 5/2 % '% ( ffl % & '% ( ffl *+, 4 "\fl F) . *" # ", "M *7 ffi ) # $ " / G ffl & ,M cffi )ffl # '# , n"J *"3 X L " % ", "M4! ffl ( Tfi * ]36 %37[. * fi 0 ' 8 # ' fi fflM fflM "8 # # O) # ( # 2009)# 8fi % : '\fl F) # # "$ J # &3) 0 8fl % M # $ ' fi # DPPH " # J # # . OM '- fflM 8 # # O) 4# X fl 2 # ", "M *"fl \J 2L " #@wfl ffi 4 +2 ) "?fi " ( Ifi%Z": _ " " ";;l) _ # ) # ]38.[
ز ن و fl م د fflر ff " ! ن fi # $ %٥ ١)٠٢'رد- | ر /. م، ffl1ی13 -دور 4 Figure 1 Antioxidant activity of chitosan films containing BPNE and MONE. fl ffiffi fflflff fi 5-2- *+$, $-( fi $% "& '() ffi fi !"# ., /-( fi 0 /$% !"# 1 2 $fl 4fl5 ffi*+$, $-( fi $% "& ." 3& , < fl ffi 16 1 ffi 5 $, 9ffi 4 fiffi ffi fl fi67 fi $% ffi'? !$7( ffl % ? ffi -@ .4, fl ffiffi /= 2 fl / 5 A 9 ffi '() ffi fi ffi *+$, $-( ? ffi *+$, $-( fi $% ffi'? % ffiffi /= B$ .4, F7 4flffi =*# 16 ffi AC ffi DA $E% fl ffiffi fflflff fi @ A $E% ? ffi fi $% Gfl ? *9 ? .? ffi ffi ?H '() ffi fi !"# 12 ? fi $% Gfl F7 fi67 ffi AC ffi A $E% =*# fi67 16 ffi = % fi67 , ., /-( ٪ 5 fi 0 /$% ? .E$flffi , 4I- fi $= 4J ." 3& ٪4 KL$ fiffi ? ? . 4flffi '() ffi fi ? /$% ? F .(p<0.05) ffi A $E% 5 fiffi E' 3& ٪4 fi 0 /$% ? , /-( ٪ 5 fi 0 B$ .(p>0.05) = ffi fiffi E' KL$ * ." ffiffi /= 0 '() ffi *+$, $-( fi $% "& , ., /-( fi 0 /$% % fi !"# % "& 4J fiffi ." 3& /* O ffi $fl 4fl5 fl P@"? *+$, $-( ffl*# /$% fi !"# 5 Q% ? 4R"S B$ 0 '() B$ . ffl*# 4J F ( D 2020) / U . ffiffi 4@) F@@T 7ffi 1'() "& (ffi $- ., fi 0 /$% !"# 2 ffi fl fi67 5 4fl5 ffi*+$, $-( fi $% , <J# fi67 15 ffi W ffi AXY AC ffi fi $% ffi'? '() F ffi .ffiffi Z , ffi fi 0 fi Gfl .4flffi =*# ffi*ff ffi B$ . ffi A $E% 5 $% fiffi E' ) /$% fi !"# ?H 4R"S fl ffiffi fflflff fi ffi Gfl ffiffi /= $-( fi $% fi fi ? fiZ 2 ., (D 2012) / ffifi6 '() ffi ]39.[ E$flffi *+$, ?%% ES ., -% $_ 2 - $,= % $a% !"# ffi fl ffi 0 ?2 % fi 4R"S 1 2 .4# 5 Z*(_ ffi /$% ffl*# ., 4R"S /ffi *# fiffi E' C -% $_ $-( fi $% fi 2 F $= % fiC #4 . ffi . .$ $ L(, fi $% b F $% *+$, /E& I?%% ES ., /ffi *# % ffiffi /= '() F B$ /$%-$,= $a% !"# Q(Z ffi 'IC c W RE % fi !"# ',? ffi H <-$ff ? / / e ]40.[ fl $flffi A'# fiE$- ffi ffid$, <$ -% % /$% 4a% !"# ffi % D(2017) ffi % g O 4fl5 ffi h 3& F= _ ., fi 0 *(", , ffi O157:H7 "% fl fl *+$, $-( "& $fl ffid$, ffi *+$, $-( fi $% Gfl /* .ffiffi Z /$% !"# A $E% 5 ffi 5 1I% ? fi 0 !"# ffi 1Jffi 4flffi =*# 6E? *(", <$ -% % ffi = % F h 3& F= ., ffid$, ] .41.[ ffi A $E% 5 4I- fi ?Fff Gfl /* fiffi
ز ن و fl م د ffi ن ffl "!رfi ff #$ 2 دور+ 1/ی/، !م -, ر+ | رد%٠٢'١ ٣4 Table5Average reduction rate of Listeriamonocytogenes count (log CFU/g) among treatments when compared together during 16 days of storage. Group I Mean Difference I-J Group J CH CH+BPNE1 CH+BPNE2 CH+MONE CH+BPNE1+M ONE CH+BPNE2+M ONE Con 1.06* 1.33* 1.69* 1.59* 1.91* 2.12* CH 0.26* 0.63* 0.52* 0.84* 1.05* CH+BPNE1 0.36* 0.26* 0.58* 0.79* CH+BPNE2 -0.10 0.21* 0.42* CH+MONE 0.32* 0.53* CH+BPNE1+MONE 0.21* ⁎Indicates a statistically significant difference (p <0.05). Figure 2 Changes of Listeria monocytogenescount of camel meat samples in different treatments during 16 days of storage at 4 °C. TBARS 6-2- 6 ffi ffl fffi fl TBARS *" +*(),, -./ fl% & '() .!" # mg MDA/kg *0 *fl* %* *), %*&( 3 * -.*/ 4( ffi5 6 20/0-22/0 ffi*)78 * *" +(),, 9( , !/ *= *fl . " 77/1 mgMDAlkg , :) +*(),, >( *3 !?*" @*A *B? " ( @ " @ *" 5 % *= )8 CA3 %7ff( 4():, ., C8 *:) -.*/ 4*( * G@*A * B? 4 % " ( IJ)*. fl % . " 88/0 mgMDA/kg , *9fl *: .(P<0.05) , ffi)78 , %7ff - )**"& %8 **. %)(P**8 CA**3 % **Q8 %**) # *O G(1397) %8*8 4*R @ " !"S B? T8 = )8 %*", 21 *:U * *6 !*/ , %(:7 , CA3 V)" & " Q8 %,( G 8 * *, %*7ff *O %fl* @ *" *B? , fl *B? )&, !WAR = !/ " Q8 **fl fi# fi** X9**& !**ff )**&, O** G@ **" %AS , fl )8 CA3 O[ (G 2013) 9fl 48 ]42 .[ !/ %*", * \*. !*/ !V8 , ] ffi73 ffi5 fl % TBARS & '() G 6 %*&( 3 * U) " ffl 4 :U 12 CA*3 *, */ >*/S fl TBARS %fi !/ * * 4(*S %*7ff * , *] ffi73 %AS = )8 *O %*", ( G 2007) *9fl @A) *fflffl ]43.[ *, ffi)78 ffi3*8 -α *)8 G* *B? % *Q8 %*) # * & G U) " ffl 4 \. !/ fl@"" *, *)8 CA*3 *, *Q(^ * B? = fl 8 3CA % *Q8 %) # !_. %fi73 `8 ffl Xfi , %(:7 * *fl * %* *fl fi fi* . /,% )&, %*7ff * , ffi*)78 * TBARS * . *& %&( 3 ffi3*8- α B? = )&, fl ,]44.[ \. !/ @""
زن و flم د ffi ن ffl "!رfi ff #$ 4 دور- 13ی1! ،م /.ر- | رد'٠٢)١%& Table6Average reduction rate of TBARS among treatments when compared together during 16 days of storage. Group I Mean Difference I-J Group J CH CH+BPNE1 CH+BPNE2 CH+MONE CH+BPNE1+M ONE CH+BPNE2+M ONE Con 0.27* 0.36* 0.43* 0.34* 0.43* 0.47* CH 0.09 0.15* 0.06 0.15* 0.20* CH+BPNE1 0.06 -0.02 0.06 0.10 CH+BPNE2 -0.09 0.001 0.45 CH+MONE 0.09 0.13 CH+BPNE1+MON E 0.04 ⁎Indicates a statistically significant difference (p <0.05). Figure 3 Changes of TBARS camel meat samples in different treatments during 16 days of storage at 4 °C. pH 7-2- flffiffl ffifffi ffi ! pH flffi*+, ffi pH . $% & '( fl7 " # flffiffl fffi !-./ ( & 0&1 ! $6ffi7 flffi*+, ffi pH 04 5/ .$& 234 5/ # ffi, ffi ( $ffi/4 04 5ffi/ *ffi3( $8ffi% ffi( ffi4 ffi! ( #* fl14/5 *+, *( pH ' *, ( & 0&1 ! '3 >4* . % (64/6 ffi? ' ffi*, -.ffi/ ffi( ffi& ffi ffi! ffi ffi( $67 24#+ ( . ffi&(2ffi Bffi( pH @ffi. ffiA8 ffi% ffi4 @ ffi% ' ffi* , -.ffi/ ( & 0&1 ! ( 2+ff CD*E -.ffi/ ( & 0&1 ! ( @. 4 % % 4 5 % ? . ffi3 ffi4 @ffi. ffiA8 4 % ffi% ffi4 5/2 % ffi? ' ffi*, 5/2 % ? ' *, -./ ! ( 2+ff CD*E +G! @ ffi. A8 4 % ? ' *, -./ H@. A8 4 % % 4 .( P>0.05) ffi3 ffi4 % 4 5 % ? ' *, -./ +G! 24ffi.L ffi6, ffifi Mffifi ( $& pH 04 5/ 2.K $.8 ' ffiffi& *ffiffi7Q& 2ffiffi& ffiffi! ffiffiN Mffiffi* ffiffi Offiffi N MffiffiP !-7 Q 2Q*fiR1 $fiff/ S% $& !1 ( H 1397) ' ffiQ! Vffi -4ffi,]45 .[ ffi&( 2ffi 2(Q !-45 N $ffi& 'ffi @ ffi% 1 % 2 % ffi? ' *, -./ , '( @ ffi% ' ffi( -.ffi/ flffi*+, ffi ( $67 *,pH Y ffi( ' @ % 2(Q Z [ Mfi ( 2 4 , ( ]ffi^_ ]46.[ ffi&( ffi 2 7/ fi \*fiR1 !*,( 2fiffi+/ 2ffi.1 ffi? ' ffi*, -./ " 5 (2013) ' Q! , $67 # ' pH 0!, a8( OE $& b -.ffi/ (ffi, H(2017) ' Q! '4cE ]43[. & fl*+, ( ffiffiffi? 5fi.ffiffiffi% Mffiffiffi* 2ffiffiffi7,(, ' ffiffiffi*, $ffiffiffi4d, ffiL 2%( *& , eb $& 2ff6ffl ! # 9/5 ffi(6/6 ffi? $& ! 2 pH ' 5 H @ ffi% ffi? 2, ffiE -./ pH ' 5 2fi , 1 04 5/ ffi ( ffi# 14 flffiffl flffi*+, $6ffi7 ffi( ffiA8 ]41.[ ( fl*+, 41 2+ff
ز ن و fl م د ffi ن ffl "!رfi ff #$ 2 دور+ 1/ی/، !م -, ر+ | رد%٠٢'١ ٣٨ Table7Average reduction rate of pH among treatments when compared together during 16 days of storage. Group I Mean Difference I-J Group J CH CH+BPNE1 CH+BPNE2 CH+MONE CH+BPNE1+MON E CH+BPNE2+MON E Con 0.14* 0.23* 0.33* 0.32* 0.41* 0.48* CH 0.08* 0.18* 0.18* 0.26* 0.34* 0.10* 0.09* CH+BPNE1 0.17* 0.25* 0.006 0.07 0.15* CH+BPNE2 0.08 0.15 CH+MONE 0.07 CH+BPNE1+MONE ⁎Indicates a statistically significant difference (p <0.05). Figure 4 Changes of pH camel meat samples in different treatments during 16 days of storage at 4 °C. 3- flffiffl ff "#$ % & '( ! &) &# %) fi !ffl #fi 12 34 56 - 7*+, ( - -!#. /0 - fi- /fi ; <ffi 6=17 ./9- 8*) &/; <)/- + 3 ./9- #- ABffl ! #9 <9C /?D "/ >8 <?2 "@ #+ &B) . # &B) E=F /?D G ; <B & '( ! &) &# %) fi !ffl 7%) #) - H #+ ! &/; <)/- % 34 "I'J #) /?D "/ >8 <?2 7C . #C ?6, K# ! 7 <9C 12 4- [1]Dawood AA. 1995. Physical and sensory characteristics of Najdicamel meat. Meat science. 39(1):59-69. [2] Soltanizadeh N, Kadivar M, Keramat J, Bahrami H, Poorreza F. 2010. Camel cocktail sausage and its physicochemical and sensory quality.International Journal of Food Sciences and Nutrition. 61(2):226- 243. [3] Kadim IT, Mahgoub O, Al-Marzooqi W. 2008. Meat quality and composition of Longissimus thoracis from Arabian camel (Camelus dromedaries) and Omani beef: A comparative study. Journal of Camelid Sciences. 1:37-47. [4] Kim SJ, Cho AR, Han J. 2013.Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation.Food control. 29(1):112-120. [5] Bouhdid S, Abrini J, Amensour M, Zhiri A, Espuny MJ, Manresa A. 2010. Functional and ultrastructural changes in Pseudomonas aeruginosa and Staphylococcus aureus cells induced by Cinnamomum verum essential oil. Journal of applied microbiology. 109(4):1139-1149. [6] Packiyasothy EV, Kyle S. 2002. Antimicrobial properties of some herb essential oils.Food Australia. 54(9):384-387. [7] Iaranathan RN. 2003. Biodegradable films and composite coatings: past, present and future. Trends in food science & technology. 14(3):71- 78. [8] Beverlya RL, Janes ME, Prinyawiwatkula W, No HK. 2008. Edible chitosan films on ready-to-eat roast beef for the control of Listeria monocytogenes. Food microbiology. 25(3):534-537. [9] Bourlieu C, Guillard V, Vallès-Pàmies B, Gontard N. 2007. Edible moisture barriers: materials, shaping techniques and promises in food product stabilization. Food Materials Science: Principles and Practice. 547-577. [10] Tavassoli-Kafrani E, Shekarchizadeh H, Masoudpour-Behabadi M. 2016. Development of edible films and coatings from alginates and carrageenans.Carbohydrate polymers. 137:360-374. [11] Zhao LM, Shi LE, Zhang ZL, Chen JM, Shi DD, Yang J, Tang ZX. 2011. Preparation and application of chitosan nanoparticles and nanofibers. Brazilian Journal of Chemical Engineering. 28(3):353-362. [12] Maghsoudlou A, Maghsoudlou Y, Khomeiri M, Ghorbani M. 2012. Evaluation of anti-fungal activity of chitosan and its effect on the moisture absorption and organoleptic characteristics of pistachio nuts.International Journal on Advanced Science, Engineering and Information Technology. 2(4):336-340. [13] Shahidi F, Arachchi JK, Jeon YJ. 1999. Food applications of chitin and chitosans. Trends in food science & technology. 10(2):37-51.
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Le effect of chitosan oral film containing Trachyspermum ammi essential oil on some chemical properties of chicken meat.Journal of Medical Science and Nutrition.16(4): 91-101.[In Persian] Antimicrobial and antioxidant effects of chitosan edible film containing nanoemulsion of Melissa officinalis L. extract and Buniumpersicum essential oil on Listeriamonocytogenes inoculated into camel meat Abstract The aim of this study was to investigate the antimicrobial and antioxidant effects of chitosan edible film containing nanoemulsion of Melissa officinalis L. extract and Bunium persicum essential oil on Listeriamonocytogenes inoculated into camel meat. The studied films were prepared using 2% chitosan and 2.5% and 5% of nanoemulsion of Bunium persicum essential oil and 4% of Melissa officinalis L.extract. The antimicrobial and antioxidant effects of coated camel meat during 16 days of storage at 4 °C with a 4-day interval (0, 4, 8, 12, 16) were evaluated. Le coated portions were chemically evaluated. Most of the essential oil compounds include: cuminaldehyde (24.37%), γ-Terpinene (19.99%), and P-cymene (9.71%). Le MIC of Melissa officinalis L. extract and Bunium persicum essential oil against L. monocytogenes were 1% and 0.25%, respectively. The antioxidant effects of films by DPPH method showed that the addition of essential oils and extracts increases the antioxidant properties of films. The antimicrobial effect of films by disk diffusion method, the largest diameter of growth inhibition zone (17.15 ± 0.16) was related to chitosan film containing 5% Bunium persicum essential oil and 4% Melissa officinalis L. extract. The average count of L.monocytogenes in the control treatment was higher than the other treatments. The results of TBARS showed that the antioxidant properties of films containing Bunium persicum essential oil and Melissa officinalis L. extract was higher than the control sample. The pH level in the samples coated with chitosan film containing 5% Bunium persicum essential oil and 4% Melissa officinalis L. extract was lower than the other treatments. In general, the prepared films have good antimicrobial and antioxidant properties against L. monocytogenes, which increase with the addition of plant compounds. Keywords: Listeria monocytogenes, Chitosan, Buniumpersicum, nanoemulsion, MelissaofficinalisL. extract, Camel meat