11th International Conference

11th International Conference on Nutrition and
Physical Activity in Ageing, Obesity and Cancer

(NAPA2022)

“Power of Personalized Health and Wellness”
December 15-17, 2022

The Empress Chiang Mai Hotel

Organized by
Faculty of Pharmacy, Chiang Mai University, Thailand

Hosted by

Table of Contents

Welcome Message ....................................................................................................................4
Welcome Message ....................................................................................................................5
Welcome Message ....................................................................................................................6
Welcome Messages...................................................................................................................7
Advisory Committee ................................................................................................................8
Scientific Program .................................................................................................................10

DAY 1: Thursday, December 15, 2022................................................................................ 10
DAY 2: Friday, December 16, 2022 .................................................................................... 11
DAY 3: Saturday, December 17, 2022 ................................................................................ 12
ABSTRACTS – INVITED SPEAKERS ..............................................................................13
ORAL PRESENTATION .....................................................................................................37
List of On-site Oral Presentation.......................................................................................... 38
List of Online Oral Presentation........................................................................................... 39
POSTER PRESENTATION .................................................................................................40
List of On-site Poster Presentation....................................................................................... 41
List of Online Poster Presentation........................................................................................ 42
Venue.......................................................................................................................................44
Sponsorship ............................................................................................................................45

Welcome Message

Dear Friends, Colleagues, Distinguished Speakers and all Participants,
As a host and a co-chair of this meeting, we are delighted to welcome you all to the 11th
International Conference on Nutrition and Physical Activity 2022 (NAPA2022), during
December 15-17, 2022 in Chiang Mai, Thailand.
The theme of the conference is “Power of Personalized Health and Wellness.” Due to the global
pandemic of COVID-19, the NAPA2022 will be delivered in a hybrid event format, combining
an in-person conference experience with a virtual component. We are working with our venue
partner, the Empress Hotel Chiang Mai, to ensure a safe and secure environment for on-site
participants.
The conference provides a premier forum for scientists, pharmacists, physicians, nutritionists,
biologists and other specialists, both locally and abroad to exchange and share their experiences
and research results about all the aspects of health care, nutritional and food science. We assure
to bring you a stimulating scientific program, which will include an outstanding line-up of
speakers and oral, poster presentations.
In the meantime, I would like to express many sincere thanks to the organizing committee and
other co-chairs of NAPA2022: Professor Malyn Ungsurungsie, President of Thai
Environmental Mutagen Society; Professor Yong Sang Song, College of Medicine, Seoul
National University; and Professor Young-Joon Surh, College of Pharmacy, Seoul National
University for their devotion and constant effort to make this conference a success. I would
also like to thank all the speakers for making great effort of sharing ideas, knowledges and
research results and to all the participants, the academic communities. We are looking forward
to your presence and wish you a pleasant stay in this amazing city of Thailand.
We look forward to welcoming everyone in Chiang Mai!

Associate Professor Supat JIRANUSORNKUL
Dean, Faculty of Pharmacy
Chiang Mai University

4

Welcome Message

Dear Friends and Colleagues,
It gives us great pleasure and pride to announce that the 11th annual conference of Nutrition
and physical activity in aging, obesity, and cancer 2022 (NAPA2022) will be placed on
December 15-17, 2022, in Chiang Mai, Thailand.
NAPA meeting has held since December 2009 in Jeju Island, Korea. In November 2022, NAPA
society was established with 12 executive committee members and more than 100 board
members from 30 countries. This could be possible with the help of many members.
Due to the Corona epidemic, a number of activities have been hosted virtually, making it
challenging to meet members personally. The NAPA 2022 will, however, be presented in a
hybrid event style that combines an actual conference with a virtual component. We are
working diligently to ensure the safety of all participants of NAPA 2022.
“Power of personalized Health and wellness” is our theme for this year’s meeting. To promote
public health through aging-related disease prevention including cancer, the NAPA2022 has
focused on personalized Health. Nobody can refute the reality that we are on the cusp of
entering a new era that will be characterized by more individualized approaches to the
management of our health.
At the international conference this December, we would like to discuss the most updated
research and the future perspective relevant to the role of nutrition and physical activity in the
prevention and management of aging, obesity, and cancer. Based on the success of past NAPA
conferences, NAPA 2022 will continue to provide members with the most recent research
information and a place for communication.
Finally, we would like to express our appreciation for the contributions of various sponsors and
the organizing committee. Moreover, we would also like to express many sincere thanks to the
young scientists and all the speakers for sharing their outstanding findings.
We look forward to welcoming you at the 11th annual meeting of the NAPA in Chiang Mai in
2022.

Professor Yong-Sang SONG

Department of Obstetrics & Gynecology
Gynecologic Cancer Center
College of Medicine, Seoul National University

5

Welcome Message

As one of the co-chairs of the 11th International Conference on Nutrition and Physical Activity
in Aging, Obesity and Cancer or NAPA2022 which will take place in Chiang Mai from 15th to
17th December 2022, it gives me much honor and great pleasure to convey my hearty and
warm welcome to all distinguished speakers, guests and attendees to NAPA2022.
Due to the COVID pandemic issue, some participants cannot join the event onsite but thanks
to the advanced technology to make us be able to hold a hybrid meeting, both onsite and online,
to reach a wider audience from around the world to actively participate in this event.
NAPA2022 is being hosted by our dear friends at Faculty of Pharmacy, Chiang Mai University.
This upcoming Conference will be held under the theme “Power of Personalized Health and
Wellness” to promote people’s desire to live healthier and ultimately to have a longer, higher-
quality life. We are certain that this Conference will gather and disseminate the latest
knowledge from diversified fields of expertise through several scientific presentation sessions.
During the conference I rather ensure that brilliant ideas and results from outstanding
experiences and research will be shared and discussed.
I would like to thank our active host, the Faculty of Pharmacy, Chiang Mai University. Also, I
wish to express my sincere appreciation to other co-chairs of NAPA2022: Associate Professor
Supat Jiranusornkul, Dean of Faculty of Pharmacy, Chiang Mai University; Professor Yong
Sang Song, College of Medicine, Seoul National University; and Professor Young-Joon Surh,
College of Pharmacy, Seoul National University.
Additionally, I would like to recognize and thank the NAPA2022 organizing committee, non-
committee member staff and volunteers as well as sponsors for all of their supports to make
this Conference happen.
In closing, I want to emphasize the importance of NAPA’s mission and the broad aim of this
year’s meeting, which is expected to bring the successive and successful conferences of
NAPA in the future.
Please have a very enjoyable time in Chiang Mai. We look forward to welcoming you in
Thailand for NAPA 2022.

Professor Malyn UNGSURUNGSIE

President, Thai Environmental Mutagen Society

6

Welcome Message

On behalf of our host, Chiang Mai University Faculty of Pharmacy, I would like to express
my great pleasure welcoming you to the 11th International Conference on Nutrition and
Physical Activity (NAPA) in Aging, Obesity and Cancer. The endeavors undertaken by the
NAPA 2022 Organizing Committee in gathering the multitude of world-class speakers will
contribute to our better understanding of the most recent forefront insights into the molecular
basis of health benefits of nutrition and physical activity. NAPA 2022 represents a golden
opportunity to strengthen the networking among the participants to share the most recent
scientific discoveries, technologies, research findings and exciting innovations.
The balanced nutrition and regular exercise are recognized as the most reliable and
convincing strategies. As NAPA 2022 participants, your active involvement, interaction and
contributions are important and valuable, permitting us to achieve the goals of this hybrid
international conference. It is our deepest desire that the selected oral and poster presentations
will be rewarded and will subsequently be published in globally accredited journals.
In closing, I am grateful for the numerous efforts taken by Associate Professor Dr. Supat
Jiranusornkul, Dean of the Faculty of Pharmacy, Chiang Mai University and faculty
members, staffs, and students in organizing this international conference. I would also like to
express my sincere appreciation to the sponsors who have provided generous financial
support for the successful organization of the conference and awards for our next generation
researchers.
With best wishes for an outstanding conference.

Professor Young-Joon SURH
Tumor Microenviroment, Global Core Research Center,
College of Medicine, Seoul National University

7

Advisory Committee

Supat JIRANUSORNKUL Chiang Mai University, Thailand

Malyn UNGSURUNGSIE Thai Environmental Mutagen Society, Thailand

Yong Sang SONG Seoul National University, Korea

Young-Joon SURH Seoul National University, Korea

Aroonsri PRIPREM Khon Kaen University, Thailand

Banleu SUNGTHONG Mahasarakham University, Thailand

General Organizing Committee

Chadarat AMPASAVATE Chiang Mai University, Thailand

Kang-Yell CHOI Yonsei University, South Korea

Hun Taeg CHUNG University of Ulsan, South Korea

Penkarn KANJANARAT Chiang Mai University, Thailand

Kantaporn KHEAWFU Chiang Mai University, Thailand

Chalermpong SAENJUM Chiang Mai University, Thailand

Pawitrabhorn SAMUTRTAI Chiang Mai University, Thailand

Kasirawat SAWAENGRAT Chiang Mai University, Thailand

Buntitabhon SIRICHANCHUEN Chiang Mai University, Thailand

Ornusa THAMSERMSANG Chiang Mai University, Thailand

Scientific Committee

Sasitorn AUEVIRIYAVIT National Nanotechnology Center, Thailand

Siripat CHAICHIT Chiang Mai University, Thailand

Tanpong CHAIWARIT Chiang Mai University, Thailand

Wantida CHAIYANA Chiang Mai University, Thailand

Sunee CHANSAKAOW Chiang Mai University, Thailand

Takron CHANTADEE Chiang Mai University, Thailand

Chuda CHITTASUPHO Chiang Mai University, Thailand

Aekkhaluck INTHARUKSA Chiang Mai University, Thailand

Pensak JANTRAWUT Chiang Mai University, Thailand

Periyanaina KESIKA Chiang Mai University, Thailand

8

Ruttiros KHONKARN Chiang Mai University, Thailand

Ruttiros KHONKARN Chiang Mai University, Thailand

Kanokwan KIATTISIN Chiang Mai University, Thailand

Ornanong KITTIPONGPATANA Chiang Mai University, Thailand

SubramanianThanga LEELA Chiang Mai University, Thailand

Bharathi MURUGANANTHAM Chiang Mai University, Thailand

Rungsinee PHONGPRADIST Chiang Mai University, Thailand

Sirivipa PIYAMONGKOL Chiang Mai University, Thailand

Worrapan POOMANEE Chiang Mai University, Thailand

Somjing ROONGJANG Chiang Mai University, Thailand

Mathukorn SAINAKHAM Chiang Mai University, Thailand

Phennapha SAOKHAM Chiang Mai University, Thailand

Sudarshan SINGH Chiang Mai University, Thailand

Sasithorn SIRILUN Chiang Mai University, Thailand

Bhagavathi Sundaram SIVAMARUTHI Chiang Mai University, Thailand

Singkome TIMA Phetchaburi Rajabhat University, Thailand

Pratchaya TIPDUANGTA Chiang Mai University, Thailand

Karnkamol TRISOPOL Chiang Mai University, Thailand

Prakairat TUNIT Chiang Mai University, Thailand

Wipawadee YOO-IN Chiang Mai University, Thailand

9

Scientific Program

DAY 1: Thursday, December 15, 2022

Time Activities

08:00-17:00 Registration

08:45-09:00 Opening ceremony
Welcome remark
Supat JIRANUSORNKUL (Chiang Mai University, THAILAND)
Opening remark
Yong Sang SONG (Seoul National University, KOREA)

Session 1 Chair: Hun Taeg CHUNG

09:00-09:30 Janus-Faced Role of NRF2 in Multistage Carcinogenesis
Young-Joon SURH (Seoul National University, KOREA)

09:30-10:00 Impact of dietary nutrients (functional foods/nutraceuticals) and micronutrients on COVID-19
pathologies
Chin-Kun WANG (Chung Shan Medical University, TAIWAN)

10:00-10:30 Effect of bacteria-derived extracellular vesicles on adipogenesis and lipogenesis using human
adipose derived stem cells
Yong-Sang SONG (Seoul National University, KOREA)

10:30-11:00 Coffee break & Poster presentation

Session 2 Chair: Malyn UNGSURUNGSIE
11:00-11:30 Dietary recommendations for DNA damage prevention – an update (Online)

Michael FENECH (University of South Australia, AUSTRALIA)

11:30-12:00 Precision Cancer Medicine Strategies in Targeting Tumor Metabolome
Danny N. DHANASEKARAN (University of Oklahoma Health Sciences Center, USA)

12:00-12:30 Application of Probiotic in non-communicable disease (NCD)
Chaiyavat CHAIYASUT (Chiang Mai University, THAILAND)

12:30-13:30 Lunch

Session 3 Chair: Aroonsri PRIPREM

13:30-14:00 Cancer-associated miRNAs and their therapeutics potential
Johji INAZAWA (Tokyo Medical and Dental University, JAPAN)

14:00-14:30 Blood Brain Barrier and Ethanol: From Basic Research to Applied Science (Online)
Marlyn Dianne LAKSITORINI (Universitas Gadjah Madah, INDONESIA)

14:30-15:00 Epigenetic regulation of Autophagy in Cancer by Natural Products
Ciro ISIDORO (University of Eastern Piedmont, ITALY)

15:00-15:30 Coffee break & Interactive Poster presentation (Online)
Session 4 Chair: Chalermpong SAENJUM
15:30-16:00 Halofuginone Regulates Keloid Fibroblast Fibrotic Response to TGF-β Induction

Gwenaël ROLIN (University of Bourgogne Franche Comté, FRANCE)

16:00-16:30 Salsolinol inhibits growth of human liver cancer cells through regulation of HO-1/NF-kB signaling
16:30-17:00 Hye-Kyung NA (Sungshin Women's University, KOREA)

17:00-18:00 Mechanisms of anti-neurodegenerative and anti-aging herbal extracts and their constituents for
potential innovative health products

Tewin TENCOMNAO (Chulalongkorn University, THAILAND)

Committee meeting

10

DAY 2: Friday, December 16, 2022

Time Activities

08:00-17:00 Registration

Session 1 Chair: Gwenaël ROLIN

09:00-09:30 Toward Personalized Healthcare Through Pharmacogenomics in Thailand

Chonlaphat SUKASEM (Mahidol University, THAILAND)

09:30-10:00 Capitalizing the cancer dynamic re-wiring: Evolution of the cancer precision

treatment paradigm

Siwanon JIRAWATNOTAI (Mahidol University, THAILAND)

10:00-10:30 Health Benefits of Probiotics as New Integrative Treatment Strategies for Polycystic

Ovary Syndrome (PCOS)

Sepideh ARBABI BIDGOLI (Islamic Azad University Tehran Medical Sciences,

IRAN)

10:30-11:00 Coffee break & Poster presentation session

Session 2 Chair: Kang-Yell CHOI

11:00-11:30 Phytochemicals repressing methylmercury risk

Yoshito KUMAGAI (University of Tsukuba, JAPAN)

11:30-12:00 The double-edged sword of immunogenic cell death

Marc DIEDERICH (Seoul National University, KOREA)

12:00-12:30 Updates on the medicinal mushroom Ganoderma neo japonicum Imazeki: an elixir

from Malaysia's indigenous population (Online)

Umah-Rani KUPPUSAMY (University of Malaya, MALAYSIA)

12:30-13:30 Lunch

Oral presentation - Onsite

Chair: Pratchaya TIPDUANGTA Chair: Ruttiros KHONKARN

13:30-13:45 OS-07 OS-01

Suchanan BEZUIDENHOUT Kankanit YEERONG

13:45-14:00 OS-08 OS-02

Kiattisak DUANGMAL Panipak POTHIRAT

14:00-14:15 OS-03 OS-05

Thasang THAVANAPONG Sudarshan SINGH

14:15-14:30 OS-04 OS-06

Piyatida AMNUAYKAN Pattaraporn PANRAKSA

14:30-14:45 OS-12 OS-09

Jaenjira ANGSUSING Pawarisa MANEECHAI

14:45-15:00 OS-14 OS-10

Khin Khin GYI Pichchapa LINSAENKART

15:00-15:30 Coffee break & Poster presentation

15:30-15:45 OS-15 OS-11

Tanakarn CHAITHEP Pimjai DOUNGSAARD

15:45-16:00 OS-16 OS-13

Zélie DIRAND Yeonsoo JOE

Oral presentation – Online (Zoom meeting ID: 674 901 4710)

Chair: Takron CHANTADEE and Chuda CHIITASUPHO

13:30-13:45 OO-01

Natthawat YODSURANG

13:45-14:00 OO-02

Han YU

14:00-14:15 OO-03

Nantakan AMONSUSAWAT

14:15-14:30 OO-04

Siriporn POTPROMMANEE

14:30-14:45 OO-05

Nuttapong TOCANITCHART

14:45-15:00 OO-06

11

Time Activities
Vissuta SIRIRUNGSEE
15:00-15:30 Coffee break & Poster presentation
15:30-15:45
OO-07
15:45-16:00 Treethip SUKKHO

18:00-20:00 OO-08
Neungreuthai CHOMCHOEI

Conference Banquet

DAY 3: Saturday, December 17, 2022

Time Activities

Session 1: Chair: Buntitabhon SIRICHANCHEUN

09:00-09:30 Molecular pharmacology for targeted cancer therapy: novel lead compounds targeting

cancer cell signals (Online)

Pithi CHANVORACHOTE (Chulalongkorn University, THAILAND)

09:30-10:00 Impact of aging and dietary nutrients on memory formation using model animals

(Online)

Ayako TONOKI (Chiba University, JAPAN)

10:00-10:30 Microencapsulation based on pectin and poloxamer: a way to module the curcumin

delivery
Odile CHAMBIN (Universit ́e de Bourgogne Franche-Comt é , FRANCE)

10:30-11:00 Coffee break

Session 2: Chair: Kantaporn KHEAWFU

11:00-11:30 Aspects of Sustainability in Amorphous Drug and Dosage Form Design

Thomas RADES (University of Copenhagen, DENMARK)

11:30-12:00 Oral Delivery of Therapeutic Peptides by Self-Nanoemulsifying Drug Delivery

System

Anette MULLERTZ (University of Copenhagen, DENMARK)

12:00-12:30 Awarding Ceremony and Closing Remarks

Young-Joon SURH (Seoul National University, KOREA)

12:30-13:30 Lunch

12

ABSTRACTS – INVITED SPEAKERS

13

NAPA2022 SESSION 1 (December 15, 2022)

Janus-Faced Role of NRF2 in Multistage Carcinogenesis

Young-Joon SURH

College of Pharmacy, Seoul National University, Seoul 0826, SOUTH KOREA
Email: [email protected]

Redox homeostasis is essential for the maintenance of normal physiological functions
in response to oxidative stress and other noxious stimuli. However, altered redox balance and
deregulated redox signaling are implicated in malignant progression and resistance to chemo-
and radiotherapy. The transcription factor, nuclear transcription factor erythroid 2p45 (NF-E2)-
related factor 2 (NRF2) is a master switch in the cellular antioxidant signaling and plays a vital
role in cellular protection against ROS-induced oxidative stress. The antioxidant mechanism
mediated through NRF2 activation is often usurped by cancer cells, provoking uncontrolled
amplification of antioxidant signaling. The resulting reductive stress may facilitate the
remodeling of the tumor microenvironment in a way advantageous for the autonomic growth
of cancer cells, metastasis, angiogenesis, tolerance to anticancer therapy, and self-renewal
activity of stem-like cells. Some metabolic pathways altered due to reductive stress have been
identified as major contributors to tumorigenesis. The differential effects of NRF2 on multi-
stage carcinogenesis have raised a concern about the use of NRF2 activators for cancer
chemoprevention. This presentation will highlight the opposite functions of NRF2 in normal
vs. cancerous/transformed cells with a focus on the therapeutic validity of targeting this
versatile transcription factor in the management of cancer.

Keywords: Chemoprevention; NRF2; Oxidative stress; Phytochemicals; Redox signaling

14

NAPA2022 SESSION 1 (December 15, 2022)

Impact of Dietary Nutrients (Functional Foods/Nutraceuticals)
and Micronutrients on COVID-19 Pathologies

Chin-Kun WANG

Chung Shan Medical University, 110, Sec. 1, Jianguo North Rd., Taichung, TAIWAN
Email: [email protected]

Coronavirus disease (COVID-19) in 2019 has caused global destruction and significant
loss of life. Many researchers have shown great interest in various functional
foods/nutraceuticals and dietary supplements to improve immune function and overall health,
thereby reducing the risk of COVID-19 infection. A balanced diet rich in various nutrients,
especially micronutrients, restores and prevents COVID-19-related health problems. Such as
vitamin C, vitamin D, omega-3 polyunsaturated fatty acids, probiotics and zinc, all of which
are currently undergoing clinical research. A healthy lifestyle and balanced nutrition play an
important role in the immune system. The potential and effective food bioactive substances
may be used as support or complementary intervention to reduce the risks and comorbidities
associated with COVID-19. Electrolyzed water shows innovative effects for health promotion
and also suppressing the growth of respiratory bacteria and viruses. Acid-electrolyzed water
clearly blocked the respiratory bacteria and virus, and acid-electrolyzed water greatly reduced
the risk and improved the recovery and showed an ORP-dependent manner.

Keywords: COVID-19; Immune; Balanced nutrition; Electrolyzed water

15

NAPA2022 SESSION 1 (December 15, 2022)

Bacteria-derived Extracellular Vesicles on Adipogenesis and Lipidogenesis
Using Human Adipose-Derived Stem Cells

HyunA JO1; Yong Sang SONG1,2

1 Cancer Research Institute, College of Medicine, Seoul National University, Seoul 03080,
KOREA

2 Department of Obstetrics and Gynecology, College of Medicine, Seoul National University,
Seoul 03080, KOREA

Email: [email protected]

Obesity is a major risk factor for a variety of diseases, such as type 2 diabetes,
cardiovascular diseases, and cancer. The gut microbiota appears to be a key environmental
factor contributing to obesity by affecting the host’s energy harvest and storage. Bacteria-
derived extracellular vesicles (BEVs) are lipid bilayer structures released by both gram-
negative and positive bacteria in the gut microbiota. Recent evidence has revealed that gut
microbiota can transport genetic materials and proteins from bacteria to their hosts through
BEVs. Also, BEVs play a role in the etiology of a variety of diseases, including obesity. Thus,
we discuss personalized management using BEVs for the treatment of visceral obesity. We
isolated human visceral adipose-derived stem cells (vASCs) from the visceral adipose tissues
of human donors (n=2). Meanwhile, four different BEVs (Staphylococcus aureus,
Bifidobacterium breve, Acinetobacter baumannii, and Jeotagalicoccus halotolerans) were
treated on ASC being treated with ADM (adipogenic differentiation medium). Treatment with
Staphylococcus aureus-derived EVs and Bifidobacterium breve-derived EVs inhibited lipid
accumulation in parallel with downregulation of mRNA and protein expression of the
adipogenic and lipogenic markers in vASCs. On the other hand, Acinetobacter baumannii-
derived EVs and Jeotagalicoccus halotolerans-derived EVs showed a pro-adipogenic effect on
vASC. Understanding the mechanism underlying the adipogenic differentiation of vASCs may
provide a novel insight into the therapeutic strategies for the prevention and treatment of
visceral obesity. These findings suggest that BEVs might have the potential to be used for
personalized obesity treatment.

16

NAPA2022 SESSION 2 (December 15, 2022)

Dietary Reference Intakes for DNA Damage Prevention

Michael FENECH

University of South Australia and Genome Health Foundation, AUSTRALIA
Email: [email protected], [email protected]

DNA is the fundamental genetic code that enables the existence and proliferation of all
life forms on Earth. In humans DNA is wound and packaged on histone proteins to form 23
pairs of chromosomes. Starting from a single cell at conception, the number of cells grows
exponentially and differentiates to form the various organs of the body leading to the formation
of the fetus, the birth of a child and the growth and development into an adult. These very
complex events are dependent entirely on the capacity of the cells in the body to replicate and
repair DNA accurately and to express the genes encoded by DNA appropriately to optimize
cellular health and function. A wide range of nutrients is required as substrates or cofactors of
enzymes involved in DNA replication and repair and the fine-tuning of gene expression
epigenetically. Nutrients also play an important role in defense mechanisms to prevent DNA
damage caused by endogenous and exogenous genotoxins. In my presentation, I shall discuss
the current technologies that can be used to measure DNA damage and how these genomics
tools can be implemented to determine Dietary Reference Intakes for DNA damage prevention
in humans at all life stages.

Keywords: Nutrients; DNA damage; DNA replication; DNA repair; Dietary Reference
Intakes; Human

17

NAPA2022 SESSION 2 (December 15, 2022)

Precision Cancer Medicine Strategies in Targeting Tumor Metabolome

Danny N. DHANASEKARAN

Stephenson Cancer Center, University of Oklahoma Health Sciences Center
Oklahoma City, Oklahoma, USA
Email: [email protected]

Tumorigenesis requires metabolic reprogramming of the cells for meeting their higher
energy demands as well as for the production of anabolic intermediates. With the tumor-
specific generation of specific metabolites, defining tumor metabolome and identifying tumor-
specific-oncometabolites have a high potential for the development of effective targeted
therapy. Our metabolomic analysis of ovarian cancer cells has identified novel oncometabolites
that can be utilized for ovarian cancer therapy. Metabolomic profiling of two ethnic groups of
patients from Oklahoma and Seoul has also revealed ethnic variations that can be attributed to
genetic variations, dietary composition, and microbiota composition. Precision cancer
medicine strategies based on metabolomic profiling, cognizant of these differential ethnic
factors will be discussed.

Keywords: Tumorigenesis; Oncometabolites; Tumor specific-oncometabolites

18

NAPA2022 SESSION 2 (December 15, 2022)

Application of Probiotics in Non-Communicable Diseases (NCDs)

Chaiyavat CHAIYASUT

Innovation center for holistic health, nutraceuticals, and cosmeceuticals
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University,

Chiang Mai, THAILAND
Email: [email protected]

Probiotics are live microorganisms, which, when administered in adequate amounts,
confer a health benefit on the host. Due to the proven health benefits, the rate of probiotics
consumption has gradually increased in past decades. Meanwhile, the incidence of non-
communicable diseases (NCDs) is increasing partly due to the urbanized lifestyle. NCDs,
including diabetes, heart diseases, cancers, and chronic lung diseases, are responsible for more
than 70% of mortality worldwide. The refining of food habits and a diet rich in vegetables and
fermented foods that may contain probiotics are served to prevent and manage NCDs. Probiotic
supplementation positively regulates the metabolic activities of the host, improves the host
microbiota, and influences the gut-brain axis through several mechanisms. Obesity and
hypercholesterolemia are the major factors associated with several heart diseases. The studies
proved that the consumption of probiotic formulations improved the cholesterol profile,
inflammatory markers, and intestinal permeability in obese subjects, thereby nullifying
obesity-associated co-morbidities. Lactobacillus spp. could improve the blood lipid profile and
reduce oxidative stress in hypercholesterolemic subjects. Similarly, the consequences of
diabetes are managed through probiotics. For example, the supplementation of Lactobacillus
paracasei HII01 improved the glycemic and metabolic biomarkers and modified the gut
microbiota positively in type 2 diabetic subjects. Even though further studies are required to
turn probiotic supplements into pharmacological procedures to manage NCDs.

19

NAPA2022 SESSION 3 (December 15, 2022)

Cancer-associated miRNAs and Their Therapeutic Potential

Johji INAZAWA

Director/ Specially-appointed Professor, Research Core Center, Tokyo Medical and Dental
University (TMDU); Emeritus Professor, TMDU, JAPAN
Email: [email protected]

MicroRNAs (miRNA; miR) are small protein non-coding RNAs of approximately 22
nucleotides negatively regulating target-gene expression. In the last decade, we identified over
20 novel tumor suppressor (TS)-miRs in various cancers using function-based screening with
miR-libraries. Among those, miR-634 activates the apoptotic pathway by directly concurrent
targeting genes associated with mitochondrial homeostasis, anti-apoptosis, antioxidant ability,
and autophagy. Overexpression of miR-634 strongly induced apoptosis by directly targeting
multiple genes associated with mitochondrial biogenesis and cytoprotective processes against
apoptosis in cancer cells. The enforced expression of miR-634 using chemically modified
double-strand miR-634 mimics markedly enhanced chemotherapy-induced cytotoxicity in
cancer cells in vitro and in vivo. We investigated the therapeutic potential of miR-634 ointment
in subcutaneous xenograft tumors in nude mice and a mice model of cutaneous squamous cell
carcinoma (CSCC). In xenograft tumors of A431 cells, a human skin SCC cell line, the topical
treatment of ointment incorporated double-strand miR-634 mimics significantly reduced tumor
growth. Furthermore, the topical treatment of miR-634 ointment also inhibited tumor growth
in the carcinogen-induced mouse skin papilloma model. These findings suggest that topical
treatment of miR-634-ointment may be useful as a noninvasive and effective treatment for
CSCC as well as other types of skin-invasive cancers. Malignant melanoma (MM) is one of
the most common tumors in both humans and dogs. Recently, we have tried therapeutic
implementation of intra-tumoral miR-634 administration to spontaneous canine (CMM) and
obtained antitumor effects without any adverse events (AEs) in some CMM cases, suggesting
that possible application of topical treatment of miR-634 replacement might be effective for
human MM and other age-related tumors.

Keywords: microRNA; Cancer; miR-634; Topical treatment

20

NAPA2022 SESSION 3 (December 15, 2022)

Ethanol Restriction in Pediatric Formulation: From Concept to Practice

Marlyn D. LAKSITORINI

Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada,
Yogyakarta, INDONESIA, 55281

Institute of Halal and Industry System, Universitas Gadjah Mada, INDONESIA, 55281
Email: [email protected]

Ethanol is frequently used as a cosolvent in both adult and pediatric oral liquid dosage
formulations such as syrups and elixirs. In some cases, even when the drug product is intended
for adult use, indication and dosing information for children 2-6 years is present on the label.
The presence of ethanol as a pharmaceutical excipient is not limited to prescription-based
drugs but is also present in many OTC and herbal medication products. The presence of
ethanol in pediatric formulations is concerning as expression of ethanol metabolizing
enzymes, such as aldehyde dehydrogenase (ADH) and CYP2E1, are incomplete and do not
become fully expressed until two years of age. The incomplete expression of ethanol
metabolizing enzymes was thought to be the underlying cause of the increase in blood alcohol
concentration (BAC) in infants taking ethanol-based formulations. Since December 1994, the
FDA has recommended the maximum ethanol concentration in oral liquid pediatric
formulations to be below 0.5% v/v. In addition, FDA guidance suggested the resulting BAC
should be 25 mg/dL or less after single-dose administration. It is known that the BAC
concentration between 20-50 mg/dL starts causing CNS depression. In line with the FDA,
European countries, through EMEA set an even lower limit requiring BAC of less than 1
mg/dL after a single intake. Currently, the maximum ethanol content in pediatric formulations
has not been regulated in Indonesia. Our studies on ethanol content in cough-cold syrups for
the pediatric market in Indonesia showed a wide range of ethanol concentrations ranging from
0-10% v/v. Using the updated Windmark equation, it was predicted that the ethanol exposure
level in a two-year-old infant taking a cough syrup containing 10% v/v ethanol at the
recommended dose of 5 ml would result in a BAC of 13-15 mg/dL. While this is considered
below the threshold, if the parent accidentally administered the wrong dose, for example, 10
ml instead of 5 ml, it may result in a BAC above 25 mg/dL. Furthermore, if the child
accidentally ingested half or full volume of the syrup, the BAC might be far above the FDA -
suggested limit. These findings suggest the need for both regulatory bodies and the
pharmaceutical industry to initiate ethanol restriction in the pediatric formulation.

21

NAPA2022 SESSION 3 (December 15, 2022)

Epigenetic Regulation of Autophagy in Cancer by Natural Products

Alessandra FERRARESI1; Letizia VALLONO1; Andrea ESPOSITO1; Amreen
SALWA1; Chinmay MAHESHWARI1; Beatrice GARAVAGLIA1; Chiara VIDONI1;

Danny N DHANASEKARAN2; Ciro ISIDORO1

1 Laboratory of Molecular Pathology, Department of Health Sciences,
Università del Piemonte Orientale “A. Avogadro”, Novara, ITALY

2 Stephenson Cancer Center, The University of Oklahoma Health Sciences Center,
Oklahoma City, OK, USA

E-mail: [email protected]

Autophagy is a lysosome-driven catabolic pathway for the degradation of redundant or
damaged and non-functional cellular components. The cargo is recognized by p62/SQSTM1
and sequestered within a double-membrane vacuole named the autophagosome that will
eventually fuse with lysosomes to form the autolysosomes, wherein the cargo is fully degraded
by lysosomal cathepsins and other hydrolytic enzymes. The elementary molecules are then
translocated in the cytosol and reutilized for a new synthesis. The autophagy pathway is under
the control of the PI3KC1-AKT-mTORC1 and the LKB-AMPK- pathways that both impinge
on the ULK1 complex that ultimately triggers the BECLIN1-PI3KC3 autophagy interactome.
Altogether, 34 ATGs have been identified as part of the core autophagic machinery. Genetic
and Epigenetic alterations of ATG genes and as well as that of genes controlling the signaling
pathways have been reported in cancer and have been shown to be mechanistically linked to
the malignant phenotype. Here we present novel non-coding RNAs involved in the epigenetic
regulation of autophagy that impacts on cancer behavior such as proliferation, migration, and
dormancy. More importantly, we report on polyphenol nutraceuticals that can modulate the
expression of such non-coding RNAs, thus opening new avenues for the epigenetic therapy of
cancer.

Keywords: Cancer; Nutraceuticals; micro RNA

22

NAPA2022 SESSION 4 (December 15, 2022)

Halofuginone Regulates Keloid Fibroblast Fibrotic Response to TGF-β
Induction

Pierre MARTY1,2; Brice CHATELAIN2; Thomas LIHOREAU3; Marion TISSOT1;
Zélie DIRAND 1; Philippe HUMBERT1; Clémence SENEZ1; Eleonora SECOMANDI4;

Ciro ISIDORO4*; Gwenaël ROLIN1,3

1 Univ. Bourgogne Franche-Comté, INSERM, EFS BFC, UMR1098, RIGHT Interactions
Greffon-Hôte-Tumeur/Ingénierie Cellulaire et Génique, F-25000 Besançon, FRANCE
2 Service de Chirurgie Maxillo-faciale, Stomatologie et Odontologie Hospitalière, CHU
Besançon, F-25000 Besançon, FRANCE
3 INSERM CIC-1431, CHU Besançon, F-25000 Besançon, FRANCE
4 Laboratory of Molecular Pathology, Department of Health Sciences, Università del
Piemonte Orientale "Amedeo Avogadro", Novara, ITALY

Keloids are characterized by increased deposition of fibrous tissue in the skin and
subcutaneous tissue following an abnormal wound healing process. Although keloid etiology
is yet to be fully understood, fibroblasts are known to be key players in its development. Here
we analyze the antifibrotic mechanisms of Halofuginone (HF), a drug reportedly able to inhibit
the TGF-β1-Smad3 pathway and to attenuate collagen synthesis, in an in-vitro keloid model
using patient-derived Keloid Fibroblasts (KFs) isolated from fibrotic tissue collected during
the “Scar Wars” clinical study (NCT NCT03312166). TGF-β1 was used as a pro-fibrotic agent
to stimulate fibroblasts response under HF treatment. The fibrotic related properties of KFs,
including survival, migration, proliferation, myofibroblasts conversion, ECM synthesis and
remodeling, were investigated in 2D and 3D cultures. HF at 50nM concentration impaired KFs
proliferation, and decreased TGF-β1-induced expression of α-SMA and type I procollagen
production. HF treatment also reduced KFs migration, prevented matrix contraction and
increased the metallo-proteases/inhibitors (MMP/TIMP) ratio. Overall, HF elicits an anti-
fibrotic contrasting the TGF-β1 stimulation of KFs, thus supporting its therapeutic use for
keloid prevention and management.

Keywords: Halofuginone; Keloid; Myofibroblasts; TGF-β; Fibrosis

23

NAPA2022 SESSION 4 (December 15, 2022)

Salsolinol Inhibits Growth of Human Liver Cancer Cells Through
Regulation of HO-1/NF-κB Signaling

Seah PARK1; Hyunjeong OH1; Hongkyung YANG1; Young-Joon SURH2;
Dae-Yong KIM3; Hye-Kyung NA1,4

1 Department of Future Applied Sciences, College of Natural Sciences, Sungshin
Women’s University, Seoul 01133, SOUTH KOREA

2 College of pharmacy, Seoul National University, Seoul 08826, SOUTH KOREA
3 College of Veterinary Medicine, Seoul National University, Seoul 08826, SOUTH

KOREA
4 Department of Food Science and Biotechnology, College of Knowledge-Based Services

Engineering, Sungshin Women’s University, Seoul 01133, SOUTH KOREA
Email: [email protected]

Salsolinol (SAL), 1-methyl-6.7-dihydroxy-1,2,3,4- tetrahydroisoquinoline, is a major

catechol alkaloid which is generated by non-enzymatic Pictet-Spengler condensation or

enzymatic reaction of dopamine with acetaldehyde. Although SAL has been speculated to be
associated with the development of Parkinson’s disease and alcoholism, other pharmacological

effects remain largely unknown. In this study, we found that SAL induced the expression of

heme oxygenase-1 (HO-1) in concentration- and time-dependent manners in human hepatoma

SK-Hep1 cells. Phosphorylation and nuclear translocation of Nrf2, a major transcription factor

responsible for the regulation of HO-1 expression, were induced by SAL. siRNA silencing of

Nrf2 abolished the SAL-induced HO-1 expression. Phosphorylation of Nrf2 induced by SAL
was found to be mediated by Akt. SAL inhibits the phosphorylation of IKK α/β, IκBα and

nuclear translocation of p65 in SK-Hep1 cells. Moreover, SAL suppresses the expression of

cyclooxygenase-2 while the expression of 15-hydroxyprostaglandin dehydrogenase was

increased. SAL also inhibits the colony-forming activity of SK-Hep1 cells, which was

abolished by N-acetly cysteine (NAC). NAC also abrogated the inhibitory effects of SAL on
NF-κB signaling as well as activation effects on Nrf2/HO-1 axis. In addition, intraperitoneal

injection of SAL suppresses tumor growth in mouse xenograft implanted with SK-Hep1 cells.

Further, SAL suppressed the expression of PCNA, Ki-67, and COX-2, while expression of HO-

1 was enhanced in the xenograft tumor. These findings suggest that SAL activates the
Nrf2/HO-1 signaling, but blocks the NF-κB signaling, which may contribute to its anti-

carcinogenic activities in liver cancer development.

Keywords: Salsolinol; NF-κB; Nrf2-HO-1; Liver cancer; Anti-cancer

24

NAPA2022 SESSION 4 (December 15, 2022)

Mechanisms of Anti-neurodegenerative and Anti-aging Herbal Extracts
and Their Constituents for Potential Innovative Health Products

Tewin TENCOMNAO

Natural Products for Neuroprotection and Anti-Ageing (Neur-Age Natura) Research Unit,
Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330,
THAILAND

Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn
University, Bangkok 10330, THAILAND
Email: [email protected]

Recognized as one of the fastest-ageing countries in the world, the proportion of the
Thai population aged 60 and over is projected to increase from 13% in 2010 to 33% in 2040.
Aging is a well-known risk for several diseases, such as non-communicable diseases (NCDs)
and neurodegenerative diseases (NDs). NDs are mainly caused by the progressive death of
neurons. The disruption of glutamate gradients is one of the major causes of neuronal apoptosis
in NDs. Excessive extracellular glutamate induces oxidative stress, followed by triggering
mitochondrial dysfunction and mitophagy influx. Elevated glutamate concentration also
induces endoplasmic reticulum (ER) stress, which enhances the phosphorylation of mitogen-
activated protein kinase (MAPK) signaling pathway (extracellular signal-regulated kinase
(ERK) and p38) and a specific mechanism called unfolded protein response (UPR). The
activated pathways induce C/EBP Homologous Protein (CHOP) upregulation, which triggers
neuronal apoptosis. My laboratory aimed to investigate the neuroprotective effect of various
medicinal extracts and their constituents using different approaches such as cell viability,
immunofluorescence staining, flow cytometry, and Western blot analysis. Our group found that
extracts and compounds significantly attenuated glutamate-induced neuronal apoptosis by
reducing reactive oxygen species (ROS) accumulation. Extract treatment also ameliorated
mitochondrial dysfunction and mitophagy overactivation according to the results of
mitochondrial membrane potential (MMP), ATP production analysis, and determining
mitophagy-related features, respectively. We also found that herbal treatment significantly
suppressed glutamate-induced MAPK-activated ER stress regarding calcium influx (Fluo-4
AM), p-ERK/ERK, p-p38/p38 and CHOP expression. Therefore, extracts and compounds were
demonstrated to serve as potential candidates for the development of innovative health products
to fight against glutamate-induced excitotoxicity in hippocampal neurons.

Keywords: Neuroprotection; Anti-aging; Neurodegenerative disease; Natural Product;
Glutamate-induced toxicity

25

NAPA2022 SESSION 1 (December 16, 2022)

Toward Personalized Healthcare Through Pharmacogenomics in Thailand

Chonlaphat SUKASEM

Division of Pharmacogenomics and Personalized Medicine, Department of Pathology,
Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, THAILAND

Pharmacogenomics Section, Bumrungrad Genomics Institute, Bumrungrad
International Hospital, Bangkok, THAILAND
Email: [email protected]

This session will provide an overview of the current pharmacogenomics practices and
research in Thailand, address the challenges and lessons learned from delivering clinical
pharmacogenomic services in Thailand, emphasize the pharmacogenomics implementation
issues that must be overcome, and identify current pharmacogenomic initiatives and plans to
facilitate clinical implementation of pharmacogenomics in Thailand. Ever since the
pharmacogenomics research began in 2004 in Thailand, a multitude of pharmacogenomics
biomarkers associated with drug responses have been identified in the Thai population, such as
HLA-B*15:02 for carbamazepine and ox-carbazepine-induced SJS/TEN, HLA-B*58:01 for
allopurinol-induced SCARs and HLA-B*13:01 for dapsone-induced SCARs and
cotrimoxazole-induced DRESS. Moreover, several pharmacogenomics studies investigating
the relationships of genetic polymorphisms in drug-metabolizing enzymes (DMEs) and
transporter with pharmacokinetic and drug responses in the Thai population have been
conducted such as CYP2D6 (*10) for tamoxifen efficacy, UGT1A1(*6, *28) for irinotecan-
induced toxicity and TPMT (*3C) and NUDT15 (*2, *3) for Thiopurine drugs-induced
myelosuppression and SLCO1B1 with statins-induced rhabdomyolysis etc. Recently, the
Genomics Thailand Initiative (GTI) was established by the Thai government to sequence the
genomes of 50,000 Thai people and build a genomics library to help further research and
service in fields such as precision medicine and pharmacogenomics and developing guidelines
for best practice and a regulatory framework. One of the important goals of the GTI is to
determine the role of genetic variability in the therapeutic response in 14 therapeutic areas and
ensure the generation of the scientific evidence needed to move the concept of precision
medicine into clinical practice. The future of pharmacogenomics-guided therapy in clinical
settings across Thailand appears promising because of the availability of evidence of clinical
validity of the pharmacogenomics testing and support for reimbursement of
pharmacogenomics testing. The successful implementation of pharmacogenomics in routine
clinical practice will improve drug efficacy and reduce adverse drug reactions and decrease the
overall cost of health care with the reduction in adverse drug reactions.

Keywords: Pharmacogenomics; Thailand; Clinical decision support; Electronic health records
(EHR); Precision Medicine

26

NAPA2022 SESSION 1 (December 16, 2022)

Capitalizing the Cancer Dynamic Re-wiring: Evolution of the Cancer
Precision Medicine Paradigm

Siwanon JIRAWATNOTAI

Department of Pharmacology, and Siriraj Center of Research Excellent in Systems
Pharmacology, Faculty of Medicine, Siriraj Hospital, Mahidol University, THAILAND

Email: [email protected]

In the era of personalized medicine, the design and development of anti-cancer drugs
that are specifically targeted to individuals or sets of genes or proteins have been an active
research area. However, after initially conferring beneficial effects, this drug-induced inhibition
in cell function pathways can inadvertently activate drug-induced up- and down-regulation of
feedback loops, resulting in the re-wiring in the molecular network and causing drug resistance.
Hence, the targets or their combined signatures should change in accordance with the evolution
of the network over the course of treatment. This suggests the need for a dynamic targeting
strategy as well. To understand such drug-driven molecular dynamic, we used the acquired
vulnerability screenings to identify the acquired targets of the multi-drug resistant
cholangiocarcinoma cells. We found that under pressure from Gemcitabine/Cisplatin treatment,
the cancer cells re-wired the cancer pathways and acquired vulnerability to the small-molecule
second mitochondrial-derived activator of caspases (SMAC) mimetics LCL161 and
Birinapant. The observed acquired vulnerability was found to be associated with upregulation
of an inhibitor of apoptosis protein 2 (cIAP2), a known target of SMAC mimetics. LCL161 or
cIAP2-shRNA downregulated cIAP2 and restored the sensitivity to GEM/CIS in GEM/CIS-
resistant CCA cell lines and in in vivo GEM/CIS-resistant xenograft models. A strong synergic
effect was observed when LCL161 was added to GEM/CIS. Interestingly, this synergism was
also observed in drug-naïve CCA cell lines, xenografts, and patient-derived.

27

NAPA2022 SESSION 1 (December 16, 2022)

Health Benefits of Probiotics as New Integrative Treatment Strategies for
Polycystic Ovary Syndrome (PCOS)

Sepideh Arbabi BIDGOLI1, Diba AHMADIAN1, Shahla CHAICHIAN2

1 Department of Toxicology and Pharmacology, Faculty of Pharmacy and
Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University
2 Endometriosis research center, Iran University of Medical Sciences, Tehran, Iran

Email: [email protected]

Hormonal and inflammatory mechanisms are both involved in the pathogenesis of
polycystic ovarian syndrome (PCOS), which is one of the most prevalent metabolic disorders
among women of reproductive age. We aimed to evaluate the comparative efficiency and safety
of short-term oral co-administration of lactobacillus probiotics and the standard treatment of
PCOS by focusing on the histopathological parameters and serum levels of luteinizing hormone
(LH), follicle-stimulating hormone (FSH), testosterone, and nuclear factor kappa B (NF-κB)
as well as some herbal supplements e.g., Korean Red Ginseng (KRGE), Curcumin and
Lycopene with confirmed anti-inflammatory or hormonal properties. Evaluation of the
efficiency of study subjects in a PCOS rat model was established by oral gavage of letrozole
(1 mg/kg) for 21 days. The serum levels of LH, FSH, testosterone, and NF-κB were measured,
and the morphological features and differences of the ovaries were examined in each group
using a light microscope before and after 14 days of treatment with oral regimens of study
subjects in the second phase of our study (Systematic Review) efficacy and safety of probiotics
and herbal supplements were assessed using eligible Randomized clinical trials (RCTs). Our
necropsy and histopathological evidence in the animal part of the study suggests the efficacy
of probiotics, Korean Red Ginseng Extract, and curcumin as a novel integrative medicine
against abnormal multiple follicular cysts. However, antiandrogenic and anti-inflammatory
effects were only seen in animals that were administered a combination of supplements and the
standard regimen. The systematic review of human and animal data indicated that consuming
probiotics containing lactobacillus strains (reuteri, acidophilus, gasseri, rhamnosus,casei) can
improve the lipid profile, glycemic profile, inflammatory factors, hormonal profile, clinical
symptoms, and BMI of patients. In addition, these strains were considered safe for human use
and didn’t not induce any harmful properties, due to hematologic, biochemical, histology, and
in vitro safety studies. Adding lactobacillus strains probiotics to the polycystic ovary syndrome
treatment regimen could improve symptoms and increase treatment efficacy and patients'
quality of life. In addition, based on validated human data, probiotics are safe and do not add
any side effects to the standard treatment.

28

NAPA2022 SESSION 2 (December 16, 2022)

Phytochemicals Repressing Methylmercury Risk

Yoshito KUMAGAI

Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai,
Tsukuba, Ibaraki 305-8575, JAPAN
Email: [email protected]

Methylmercury (MeHg) is an environmental electrophile that reacts readily with
protein thiols, resulting in the formation of protein adducts involved in structural change,
inhibition of enzyme activity and toxicity. However, small molecule nucleophiles such as
glutathione (GSH) and reactive sulfur species (RSS) are able to trap MeHg, thereby forming
the GSH and sulfur adducts with less toxicity. This suggests that enhancement of these
nucleophiles is a strategy to reduce MeHg risk. We reported previously that pretreatments with
sulforaphane and 6-methylsulfinyl isothiocyanate contained in broccoli sprouts and wasabi,
respectively, repress MeHg-mediated toxicity in vitro and in vivo through activation of Nrf2
because this transcription factor cooperatively regulates gene expressions of GSH synthesis,
GSH adduct formation and excretion of GSH adduct into extracellular space. We subsequently
found that deletion of CSE catalyzing RSS production increases MeHg toxicity in vitro and in
vivo due to the reduction of sulfur adduct formation of MeHg, suggesting that enhancement of
by a phytochemical including sulfane sulfur atoms would be associated with reduced MeHg
risk. In the symposium, I introduce a recent study regarding the characterization of aliphatic
chemicals to form sulfur adduct of MeHg from garlic and reduce MeHg risk caused by hexane
extract of garlic.

Keywords: Methylmercury; Garlic; Sulfane sulfur; Reactive sulfur species; Sulfur adduct

29

NAPA2022 SESSION 2 (December 16, 2022)

The Double-edged Sword of Immunogenic Cell Death

Marc DIEDERICH

Department of Pharmacy, College of Pharmacy, Seoul National University,
Seoul 151-742, SOUTH KOREA
E-mail: [email protected]

Apoptosis and autophagy were traditionally considered as the most prominent cell
death or cell death-related mechanisms. By now multiple other cell death modalities were
described and most likely involved in response to epigenetic treatments. It can be hypothesized
that necrosis-related phenotypes triggered by various treatments or evolving from apoptotic or
autophagic mechanisms provide a more efficient therapeutic outcome depending on the cancer
type and genetic phenotype of the patient. In fact, the recent discovery of multiple regulated
forms of necrosis and the initial elucidation of the corresponding cell signaling pathways
nowadays are important tools to clarify the immunogenic potential of non-canonical forms of
cell death induction. This presentation will cover the effect of epigenetically active compounds
and highlight their activity leading to non-canonical or immunogenic cell death.

30

NAPA2022 SESSION 2 (December 16, 2022)

Updates on the Medicinal Mushroom Ganoderma neo japonicum Imazeki:
An Elixir from Malaysia's Indigenous Population.

Kuppusamy UR1, 2; Lau Meng FEI1, 2; Sarasvathy SUBRAMANIAM1, 2; Chua Kek
HENG1, 2; Vikineswary SABARATNAM1, 2, 3

1 Department of Biomedical Science, Faculty of Medicine, University of Malaya,
50603 Kuala Lumpur, MALAYSIA

2 Mushroom Research Center, 3Institute of Biological Sciences, Faculty of Science,
University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Email: [email protected]

Mushrooms have been valued as food and medicine for countless generations by people
worldwide. Ganoderma mushrooms, which include over 400 species worldwide, have been
used in Oriental medicine since ancient times. Of these, G. lucidum is among the most widely
used and explored for its medicinal properties including anticancer, anti-hyperglycaemia, anti-
inflammation, antioxidant and antivirus. Ganoderma neo-japonicum is an annual polypore
mushroom found in Malaysia on decaying clumps of Schizostachyum brachycladium (a
tropical bamboo). It has a laccate basidiocarp with a long slender stipe and is considered one
of the less explored Ganoderma sp. The Malaysian indigenous tribes, including the Temuans
and Temiars, use the basidiocarps of G. neo-japonicum to treat various ailments and to improve
body wellness. Our team at the University of Malaya Mushroom Research Centre was a pioneer
in both domesticating and exploring its medicinal properties to validate the traditional claims,
particularly in treating diabetes, cancer, neuronal health, and maintaining overall health, which
may potentially serve as an elixir of health. This presentation will highlight some of our
research findings from our exploration of this mushroom as well as its future prospects.

Keywords: G. neo japonicum; Medicinal properties

31

NAPA2022 SESSION 1 (December 17, 2022)

Molecular Pharmacology for Targeted Cancer Therapy: Novel Lead
Compounds Targeting Cancer Cell Signals.

Pithi CHANVORACHOTE

Center of Excellence in Cancer Cell and Molecular Biology, Faculty of Pharmaceutical
Sciences, Chulalongkorn University, Bangkok 10330, THAILAND

Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences,
Chulalongkorn University, Bangkok 10330, THAILAND
Email: [email protected]

Advance in cancer cell biology and molecular cell signaling offers a new approach to
the drug discovery with the promising link to personalized cancer therapy. So far, a number of
molecularly targeted compounds have been revealed to have a potential benefit for cancer
management via the activities involving specific interaction with the molecular targets in the
cancer cells regulating growth and metastasis. As c-Myc is an important transcription factor
facilitating cell growth, survival, metastasis, and reprograming, that have been found to be
dysregulated or aberrantly expressed in 70% of human cancers, making it one of the most
important human onco-proteins and a promising molecular target for anti-cancer drugs. We
investigate the novel compound EMD (N, N-Bis (5-Ethyl-2-Hydroxybenzyl) Methylamine)
that can interact and induce c-Myc degradation via a ubiquitin-proteasomal mechanism, and
the modifications of molecular moieties of their structure provide the insight of pharmacophore
important for the c-Myc targeting action. In addition, the modification of the compound
structure with the knowledge of SAR offers better activity against the cancer stem cell
population in lung cancer cells. Furthermore, we investigated the effect of several compounds
derived from natural products for their potential in the regulation of other cancer signals
directing cancer growth and metastasis. Norcycloartocarpin, Ovalitenone, and Pongol Methyl
Ether, a plant-derived pharmacological active agent, were demonstrated to interact with Protein
kinase B (Akt) molecule in lung cancer cells and inhibit the protein function. The utilization of
molecular docking offers information on the affinity and binding energy of the compound and
the protein with further information useful for the structural design as a lead compound. As
cancer is a disease depending on the status of gene alterations related to the defected cell
signaling, inhibition of cancer aggressive signals specific to the patient’s gene information
could be a promising way to a personalized treatment designed and benefit the clinical
outcome.

Keywords: Drug discovery; c-Myc; Akt; EMD; Structure-activity relationship (SAR)

32

NAPA2022 SESSION 1 (December 17, 2022)

Impact of Aging and Dietary Nutrients on Memory Formation Using Model
Animals

Ayako TONOKI1; Tong YUE2; Kotomi ONUKI2, Aoi YANAGIHARA2,
Jiang MINRUI2, Motoyuki ITOH2

1 Graduate School of Pharmaceutical Sciences, Chiba University,
1-8-1 Inohana, Chuo-ku, Chiba, JAPAN

2 Graduate School of Pharmaceutical Sciences, Chiba University, JAPAN
Email: [email protected]

Age-related memory impairment (AMI) has been reported in many species, such as
humans, mice, and Drosophila, however the mechanism remains unclear. Although several
genes responsible for AMI have been identified through age-associated expression changes,
few attempts have been made to comprehensively identify AMI-related factors. Drosophila is
suitable for AMI research because of its short lifespan, easy acquisition of aged individuals,
and simple evaluation of memory ability. We performed an integrative analysis of age-related
expression changes obtained from RNA-seq data of young and aged fly heads and memory
regulatory genes obtained from genome-wide comprehensive screening. We identified gycβ, a
subunit of soluble guanylyl cyclase (sGC), an intracellular nitric oxide (NO) receptor. Genetic
or pharmacological inhibition of sGC and NO synthase (NOS) has been shown to partially
reverse memory loss in aged individuals. These results suggest that age-related dysregulation
of the NO-sGC pathway may cause AMI. The risk of age-related memory impairment is also
increased by dietary habits such as a high-fat diet (HFD). We recently established a model of
memory decline induced by HFD in Drosophila. We will discuss how aging and dietary
nutrients impact memory formation.

Keywords: Aging; Memory; Nitric oxide; High-fat diet; Drosophila

33

NAPA2022 SESSION 1 (December 17, 2022)

Microencapsulation Based on Pectin and Poloxamer: A Way to Module the
Curcumin Delivery

Odile CHAMBIN1; Niphattha AODSA2; Pattaraporn PANRAKSA2; Claire-Hélène
BRACHAIS1; Pensak JANTRAWUT2

1 Bourgogne - Franche Comté University, Dijon, FRANCE
2 Chiang Mai University, Chiang Mai, THAILAND
Email: [email protected]

As many bioactive compounds, curcumin is reported to present health benefits in
various domains (anticancer, anti-inflammatory, antioxidant) but its use in therapeutic is not so
easy due to its physicochemical properties, its sensitivity to the environment and its poor
bioavailability. Encapsulation is a means to overcome these problems. Therefore, we developed
an encapsulation process by ionotropic gelation by using pectin and poloxamer P407 in order
to increase curcumin solubility. In this study, we investigated the influence of different
concentrations of poloxamer P407 on curcumin solubility and on the properties of pectin beads
containing curcumin as a drug model (encapsulation efficiency, drug loading, size and shape,
and curcumin release). The solubility of curcumin was increased when the concentration of
poloxamer P407 increased in terms of an exponential relationship. Moreover, by DLS
experiments, this could be explained by an increase in curcumin micelles size. In the curcumin
pectin beads, poloxamer had an influence on the shape, which became more spherical when
the poloxamer concentration increased, but encapsulation efficiency and drug loading
decreased. However, the curcumin dissolution rate was clearly faster with a shorter lag time,
and the release mechanism was controlled by drug diffusion and polymer swelling. Thus, with
the choice of poloxamer concentration, pectin beads properties and curcumin release could be
modulated and be more suitable for the intended therapeutic use.

Keywords: Pectin; Poloxamer; Beads; Curcumin; Release

34

NAPA2022 SESSION 2 (December 17, 2022)

Aspects of Sustainability in Amorphous Drug and Dosage Form Design

Thomas RADES

University of Copenhagen, Department of Pharmacy, Solid State Pharmaceutics Group,
Universitetsparken 2, 2100 Copenhagen, DENMARK
Email: [email protected]

Sustainability has become increasingly important in all aspects of life, and drug
delivery and dosage form design should not be an exception here. One of the main pathways
for more sustainable drugs is to increase their bioavailability since lower drug doses could be
used, and fewer drugs will be excreted into the environment after oral administration.
Moreover, the production of raw materials (drugs and excipients) as well as the technologies
used to prepare dosage forms, should include sustainability as a guiding principle. In this
presentation, examples of such thinking are given on our collaborative work on amorphous
drugs and dosage forms. Specifically, the preparation and use of sustainable polymers to
prepare amorphous solid dispersions and mechanochemical methods for amorphization will be
presented.

Keywords: Sustainability; Drug delivery; Formulation

35

NAPA2022 SESSION 2 (December 17, 2022)

Oral Delivery of Therapeutic Peptides by Self-Nanoemulsifying Drug
Delivery Systems

Ramakrishnan VENKATASUBRAMANIAN1,2; Thomas RADES1,3; Anette
MÜLLERTZ1,4

1 University of Copenhagen, Department of Pharmacy, Universitetsparken
2, 2100 Copenhagen DENMARK,

2 Physiological Pharmaceutics Group, [email protected]
3 Solid State Pharmaceutics Group, [email protected]
4 Bioneer:FARMA & Physiological Pharmaceutics Group
Email: [email protected]

Oral administration of therapeutic peptides has been desired by patients and the
pharmaceutical industry since the discovery of insulin in the early 1920s. Recently, GLP-1
analogues received increased interest, and several are on the market as injectables. As these
peptides can reduce appetite and, thereby, obesity, and their target population is type-2 diabetes
patients, it is of particular interest to enable their oral administration. This has been attempted
by several companies, however, achieving bioavailability of around 1%. The relatively large
and hydrophilic nature of peptides, as well as the fact that they are nutritional molecules,
resulting in challenges for their intact absorption from the gastrointestinal tract. First, peptides
are prone to degradation by the low pH in the stomach and proteases in both the stomach and
the small intestine. Secondly, peptides have poor permeability across both the intestinal mucus
layer and membrane due to their relatively large size and hydrophilicity. Successful oral
delivery of peptides; thus, includes protection from degradation in the lumen during transit of
the stomach and the upper part of the small intestine. Further, to facilitate absorption across the
epithelium, permeation enhancers, such as decanoic acid or lyso-phospholipids, has to be
included in the delivery system or formed in the intestinal tract. In addition, it is important that
the peptide and the permeation enhancer are co-released from the delivery system and are co-
localized at the site of absorption. Self-nanoemulsifying drug delivery systems (SNEDDS)
have shown the potential to fulfill these criteria. SNEDDS are isotropic systems based on lipids
and surfactants that form nanoemulsion droplets upon dispersion in aqueous media. Recently,
we have shown that it is possible to incorporate the hydrophilic GLP-1 analogue, exenatide,
into SNEDDS, after complexing it with phospholipids or anionic lipids. This way, the peptide
is protected against proteases in the gastrointestinal tract, and more importantly, peptide
permeation is increased. We have increased the bioavailability after oral dosing to rats, to
around 5%, which is higher than what is obtained with the commercial products.

Keywords: Oral administration; Therapeutic peptides; GLP-1 analogue; Self-
nanoemulsifying drug delivery systems

36

ORAL PRESENTATION
37

List of On-site Oral Presentation Presenter

ID Title of Presentation

OS-01 Antioxidant Activity, Cytotoxicity, Irritation Property of Kankanit

Protein Hydrolysate from Acheta domesticus Using Enzymatic YEERONG

Hydrolysis

OS-02 Optimization and Development of Niosomes Containing Red Panipak POTHIRAT
Rice Extract for Cosmetic Application

OS-03 Comparison of Extracts from Various Varieties of Mitragyna Thasang
speciosa (Korth.) Havil Leaf and Their Antioxidant Activities THAVANAPONG

OS-04 Elicitors Enhance Secondary Metabolite Production in Vanda Piyatida

coerulea Cell Culture AMNUAYKAN

OS-05 Antibacterial, Anti-inflammatory, and Anticancer Activities of Sudarshan
Bioactive Fractions Isolated from Eucalyptus camaldulensis SINGH
Leaves with Hemostasis Ability of Hypromellose Fortified
Composite

OS-06 Semi-Solid Extrusion 3D Printing of Personalized Oral Films Pattaraporn

Using Carboxymethyl Cellulose from Durian Rind Wastes as a PANRAKSA
Natural-Based Biopolymer

OS-07 Wound Healing Effects of Oil from Apis mellifera Larvae Suchanan
BEZUIDENHOUT

OS-08 Adzuki Bean – Cooked or Hydrolysate: A Potential Functional Kiattisak

Food DUANGMAL

OS-09 Bouea macrophylla Griff. Peel Extract: A Promising Active Pawarisa
Agent for Antioxidant and Antibacterial Purposes MANEECHAI

OS-10 Inhibitory Effects of Rice Bran Oil and Rice Husk Extract on Pichchapa
Nitric Oxide Production, MMP-2, and 5α-Reductase LINSAENKART

Isoenzymes Expression

OS-11 Anti-aging Cosmeceutical Product Containing of Dimocarpus Pimjai

longan Leaf Extract DOUNGSAARD

OS-12 Development and Validation of HPTLC Method for Jaenjira

Determination of Beta-Amyrin and Stigmasterol in Ya-Ta-Pra- ANGSUSING

Sen Polyherbal Extract

OS-13 Filbertone-induced PERK-TFEB Activation Ameliorates Jeongmin PARK
Neurodegenerative Diseases Via Enhancing the Autophagy-
lysosomal Pathway

OS-14 Crude Fractional Extracts from Jiaogulan (Gynostemma Khin Khin GYI
pentaphyllum) Leaves Exhibit Cytotoxic and Antiproliferative
Activity on EoL-1 Leukemic Cells

OS-15 Antioxidative Activity Screening of Ethanolic Oryza sativa Tanakarn
Extracts from Several Cultivars CHAITHEP

OS-16 Keloid resolution through fibrotic fibroblasts and macrophages Zélie DIRAND
interaction study

38

List of Online Oral Presentation Presenter

ID Title of Presentation

OO-01 Response Rate of Imatinib and Prognostic Risk of Patients Natthawat
with Chronic Myeloid Leukemia in Northern Thailand YODSURANG

OO-02 The Anti-cancer Effects of Mitochondrial-Targeted Triphenyl Han YU
Phosphonium-Resveratrol Conjugate on Breast Cancer Cells

OO-03 Prevalence of Overweight and Obesity Among Undergraduate Nantakan
Students During the Early COVID-19 Pandemic in Thailand: AMONSUSAWAT
A Cross-Sectional Study

OO-04 Phytochemicals and Antioxidant Activity Studies of Cucurbita Siriporn

moschata, Lagenaria siceraria and Benincasa hispida Peel POTPROMMANEE

Extracts with Different Extraction Techniques

OO-05 Solubility and Dissolution Enhancement of Curcumin Aspartic Nuttapong

Acid Cocrystal TOCANITCHART

OO-06 Antioxidant and Antiaging Properties of Mangiferin Rich Vissuta
Extract from Mango “Talapnak” Leaves for A Cosmeceutical SIRISUNGSEE

Application

OO-07 Ethno-diversity of Medicinal Plants Used for Musculoskeletal Treethip SUKKHO
System Disorders in Cha Miang Forest in Mae Kampong
Village, Chiang Mai, Thailand

OO-08 Electrospraying of Mangiferin-loaded Cellulose Acetate Neungreuthai
Matrix for Cosmeceutical Application CHOMCHOEI

39

POSTER PRESENTATION
40

List of On-site Poster Presentation

ID Title of Presentation Presenter
PS-01
PS-02 Potential of Cleistocalyx nervosum Extract Against Acne- Tharadon
PS-03 inducing Bacteria for Cosmeceutical Application KHAMMITHAM
PS-04
PS-05 Development and Implementation of a Hospital-based Kopkan CHOOPAN
PS-06 Intermediate Care Model at Buntharik Hospital, Ubon
PS-07 Ratchathani, Thailand
PS-08
PS-09 Anti-inflammatory Activity of Wannachawee Recipe for Supreeya
PS-10 Psoriasis TANTIPAT
PS-11
Identification of Gallic Acid and Screening Antibacterial Naruemon
PS-12
PS-13 Activity of Terminalia chebula Gall Extract by Ultrasonication PERSTWONG
PS-14
PS-15 Method

New Tellurite Optical Fibers for Multimodal Imagery Claire-Hélène
BRACHAIS

Effect of Basil Seed Mucilage on Satiety and Energy Intake in Supitchlada

Healthy Women PHONGSRIKUN

Cancer Chemopreventive Properties of Thai Purple Rice and Charatda
Its Active Compound in Diethylnitrosamine-induced Rat PUNVITTAYAGUL
Hepatocarcinogenesis

Coffee Cherry Extract Mitigates Oxidative Stress and Protects Weeraya
Human Keratinocytes Against Airborne PAH-Induced PREEDALIKIT
Oxidative Damage
Sirinya TAYA
Chemopreventive Effect of 2',4'-dihydroxy-6'-methoxy-3',5'-
dimethylchalcone Against Diethylnitrosamine-Induced
Hepatocarcinogenesis

Cost-effectiveness Analysis of Pharmacist Interventions in Poukwan
Patients with Heart Failure in Thailand ARUNMANAKUL

The Modulatory Effect of Fermented Perilla frutescens (Linn.) Sorawit UPAKUT
Britton (Nga-mon) Seed Cake with Probiotic Yeast Starter
Culture on Cytokine/Chemokine Homeostasis in Skin
Keratinocyte (HaCaT) Cells

Total Phenolic Contents and Antioxidant Activities of Herbal Chatchanok

Tea following Thai Traditional Elements NUKULKIT

Network Pharmacology-based Approach and Molecular Siripat CHAICHIT
Docking to Explore the Multitarget Mechanism of Passion
Fruit (Passiflora edulis) Seed Extract

Molecular Docking of Phenolic Compounds of Longan Pathomwat
(Dimocarpus Longan Lour.) Seed for Inhibiting Protein WONGRATTANAK
Phosphatase 1 Activity as an Herbal Collagen Enhancer AMON

Cancer Chemopreventive Effect of Black Rice Leaves In In Rawiwan
Vitro And In Vivo Models WONGPOOMCHAI

41

List of Online Poster Presentation Presenter

ID Title of Presentation

PO-01 Anti-adipogenic Activity of Purified Gymnemic acids From Prapaipat
Tea Powder Prepared from Gymnema inodorum (Lour.) Decne. KLUNGSUPYA

PO-02 Biological Activities of Stingless Bees Propolis Extracts Ubon RERK-AM

PO-03 Comparison of Chemical Compounds of Cannabis sativa L: Ubon RERK-AM
Hang Suea Sakonnakhon and Hang Kra Rog Phu Phan Leaves
Extracted by Hydrodistillation and Phytonic Techniques

PO-04 Cytoprotective Effect of TISTR Strains Probiotics on Sarunya
Streptozotocin-exposed Pancreatic β cell line (RIN-5F) by
LAOVITTHAYANG
MTT Assay GOON

PO-05 Protective Effect of Ganoderma lucidum Extract Against Prapaipat
Aflatoxin B1 (AFB1) - induced Genotoxicity Evaluated Using KLUNGSUPYA
3D Hepatocyte Spheroid Model

PO-06 In vitro DNA Protection and Non-cytotoxic Activities of Thai Nantaporn PINNAK
Probiotics: L. paracasei (TISTR 2688) and L. rhamnosus
(TISTR 2716)

PO-07 Costs and Outcome of Immune Checkpoint Inhibitors in Lung Buntitabhon
Cancer at University-Affiliated Hospital in Chiang Mai, SIRICHANCHUEN
Thailand

PO-08 Effect of Physical Activities Programs on Muscular Strength Pornthep
Among People with Physical Disabilities RACHNAVY

PO-09 The Effect of Physical Activity on Sleep Duration and Sleep Pornthep
Quality Among People with Physical Disability RACHNAVY

PO-10 Investigation on Rheological Property and Filament Geometry Tanpong
of Buttermilk-mashed Potatoes to be used as a Material for 3D CHAIWARIT
Food Printing

PO-11 Anti-senescence, Antioxidant and Cytotoxicity of Some Edible Kewalin

Plants Cultivated in Lampang INTHANON

PO-12 DNA Analysis of Andrographis paniculata and Its Adulterants Ei Mon CHO
for Investigation of Herbal Medicinal Products

PO-13 Efficacy of Melatonin on Mood Disorders in Cancer: A Jannapas
Systematic Review and Meta-analysis of Randomized THARAVICHITKU
Controlled Trials N

PO-14 Formulation of Ketoconazole in situ Thermosensitive Gel Chutima CHAIWUT

PO-15 Effectiveness and Safety of High Dose Enalapril versus Athichar
Combined Low Dose Enalapril and Manidipine among CHANWUTHINUN
Patients with Type 2 Diabetes, Hypertension and Albuminuria

PO-16 Creating A Complete Nutrition Shake containing Rice, Beans, Khataleeya

and Rice Bran Oil following IDDSI Standard KAEWKOON

PO-17 Efficacy and Safety of Lubiprostone for Constipation in Suthinee
Children: A Systematic Review and Network Meta-analysis TAESOTIKUL

42

QR Code
• Poster Presentation VDO
• Poster Presentation Abstract
• Oral Presentation Abstract

43

Venue

Chiang Mai Meeting Rooms
The Empress Conventional Centre

The Second Floor

44

Sponsorship

At Viatris, we see healthcare not as it is but as it should be. We act courageously and are uniquely
positioned to be a source of stability in a world of evolving healthcare needs.
Viatris “EMPOWERS” people worldwide to live healthier at every stage of life.
We do so via

• Access: Providing high quality trusted medicines regardless of geography or circumstance
• Leadership: Advancing sustainable operations and innovative solutions to improve patient

health
• Partnership: Leveraging our collective expertise to connect people to products and services

“Blackmores Institute is the academic and professional arm focusing on research and education of
Blackmores Group which is the Australia’s leading natural health company.
Based on the vision of our founder Maurice Blackmore (1906-1977), we are passionate about natural
health and inspiring people to take control of and invest in their wellbeing.
We develop high quality products and services that deliver a more natural approach to health, based
on our expertise in vitamins, minerals, herbs and nutrients”.

45

46

N.Y.R. limited partnership Highlight Products

Facebook : N.Y.R. limited partnership / www.nyr.co.th
Tel : 02 886 6360 ต่อ 118 Email: [email protected]

Protein Simple Instruments

• Maurice : เครื่องวิเคราะห์เชิงปริมาณของการแยก การทำให้บริสุทธิ์และความ
หลากหลายในงานชีวเวชภัณฑ์ รองรับเทคนิคการวิเคราะห์ทั้งแบบ cIEF และ CE-SDS
ให้ผล Native Fluorescence ควบคูไ่ ปกบั Absorbance A280nm ตรวจวดั ได้ถงึ ระดับ
0.7ug/ml ให้ผลวิเคราะห์ความบริสุทธ์ิ IgG ภายใน 35 นาที และแยกความแตกต่าง

• MปรFะIจ:ุเพเคยี รงื่อแคงว่ 1ิเค0รนาาะทหเี ์แทลา่ นะถน้ั ่ายภาพอนุภาคหรือตะกอน ขนาด sub visible (1-70
ไมครอน) ในสารละลาย เชน่ Vaccine, liquid drug ดว้ ยกล้องจุลทรรศน์ดิจติ อล ตาม
มาตรฐานองค์การอาหารและยาแนะนำ มีความไวสูง สามารถตรวจได้ทั้ง protein
aggregate, silicone, micro-droplet, air bubble

• Jess : เครื่องวิเคราะห์แยกขนาดโปรตีน 2-440 kDa ด้วยเทคนิค Western blot แบบ
อัตโนมัติ ใช้เวลาเพียงแค่ 3 ชั่วโมงเท่านั้น ไม่ต้องเตรียมเจล ไม่มีขั้นตอนถ่ายโปรตีนลง
เมมเบรน วิเคราะห์โปรตนี แบบ SDS-PAGE, Immunoprobe: Chemiluminescence
+ Fluorescence detection, protein normalization และ Re-probe

Reagents: R&D systems / NOVUS / Tocris reagents

• Antibodies (Research/ GMP) : Flow Cytometry, IHC/ICC/IF,WB, ELISA
IRecombinant Proteins, GMP, Protein

• Cell culture media, supplements, growth factors, Organoid and cellular
matrix for 3D Cultures

Thermo Scientific Microplate Readers

• เครือ่ งอา่ นปฏิกริ ยิ าไมโครเพลทแบบสแกนความยาวคล่ืน UV-Visible พร้อม
ระบบควบคุมอณุ หภมู ิ และระบบเขยา่ รองรบั การอา่ นปฏิกิรยิ าแบบต่อเนอ่ื ง

• ควบคุมผ่านหน้าจอสีระบบสมั ผสั สามารถจดั เกบ็ ผลการอา่ นไว้ในเคร่อื ง หรอื
สง่ ผ่านไปยงั USB memory drive ได้

• มฟี ังกช์ นั่ สแกนสเปคตรมั , DNA at A260 quantitation, โปรตีนท้ังแบบ
colorimetric และ A280 รองรับการใช้งานกบั ไมโครเพลท 6-384 well และควิ
เวต

Thermo Scientific Laboratory Equipments

Centrifuge Speedvac CO2 incubator BSC class II 47

48

N.Y.R. limited partnership Highlight Products

Facebook : N.Y.R. limited partnership / www.nyr.co.th BSC class II
Tel : 02 886 6360 ต่อ 118 Email: [email protected]

Protein Simple Instruments

• Maurice : เครื่องวิเคราะห์เชิงปริมาณของการแยก การทำให้บริสุทธิ์และความ
หลากหลายในงานชีวเวชภัณฑ์ รองรับเทคนิคการวิเคราะห์ทั้งแบบ cIEF และ CE-SDS
ใหผ้ ล Native Fluorescence ควบคู่ไปกับ Absorbance A280nm ตรวจวัดไดถ้ ึงระดับ
0.7ug/ml ให้ผลวิเคราะห์ความบริสุทธิ์ IgG ภายใน 35 นาที และแยกความแตกต่าง

• ปMรFะIจุเ:พเียคงรแื่อคง่ ว1ิเ0ครนาาะทหีเท์แ่าลนะัน้ ถ่ายภาพอนุภาคหรือตะกอน ขนาด sub visible (1-70
ไมครอน) ในสารละลาย เชน่ Vaccine, liquid drug ด้วยกล้องจลุ ทรรศน์ดจิ ติ อล ตาม
มาตรฐานองค์การอาหารและยาแนะนำ มีความไวสูง สามารถตรวจได้ทั้ง protein
aggregate, silicone, micro-droplet, air bubble

• Jess : เครื่องวิเคราะห์แยกขนาดโปรตีน 2-440 kDa ด้วยเทคนิค Western blot แบบ
อัตโนมัติ ใช้เวลาเพียงแค่ 3 ชั่วโมงเท่านั้น ไม่ต้องเตรียมเจล ไม่มีขั้นตอนถ่ายโปรตีนลง
เมมเบรน วิเคราะห์โปรตีนแบบ SDS-PAGE, Immunoprobe: Chemiluminescence
+ Fluorescence detection, protein normalization และ Re-probe

Reagents: R&D systems / NOVUS / Tocris reagents

• Antibodies (Research/ GMP) : Flow Cytometry, IHC/ICC/IF,WB, ELISA
IRecombinant Proteins, GMP, Protein

• Cell culture media, supplements, growth factors, Organoid and cellular
matrix for 3D Cultures

Thermo Scientific Microplate Readers

• เครือ่ งอา่ นปฏิกิรยิ าไมโครเพลทแบบสแกนความยาวคลนื่ UV-Visible พร้อม
ระบบควบคุมอุณหภมู ิ และระบบเขย่า รองรบั การอ่านปฏกิ ริ ิยาแบบต่อเนอื่ ง

• ควบคุมผา่ นหนา้ จอสีระบบสัมผัส สามารถจัดเก็บผลการอา่ นไว้ในเครอ่ื ง หรอื
ส่งผา่ นไปยงั USB memory drive ได้

• มฟี ังก์ช่ันสแกนสเปคตรมั , DNA at A260 quantitation, โปรตีนท้ังแบบ
colorimetric และ A280 รองรับการใช้งานกับไมโครเพลท 6-384 well และ
ควิ เวต

Thermo Scientific Laboratory Equipments

Centrifuge Speedvac CO2 incubator

49

50