Hand Sanitiser by The Scientists, KMNS

JALINAN KERJASAMA















Kolej Matrikulasi Negeri Sembilan
Kementerian Pendidikan Malaysia

“THE SCIENTISTS” KOLEJ MATRIKULASI NEGERI SEMBILAN






MOHD ANIS SURYADY BIN MUSTAFHA

(PENASIHAT)









MARK HAUR JYE UMMI KALTHUM BT MANSOR
(KETUA KUMPULAN) (PENOLONG KETUA KUMPULAN)








AMAL HAYATI BT JAMALI

(SETIAUSAHA)




















NOR ASYIKIN BT MD SOM NOOR HIDAYAH BT ZAINAL OTHMAN BIN MOHD NOR NIZAHA BT ZULKEPLY ROZANA BT AHMAD ZUKRI
(AHLI KUMPULAN) (AHLI KUMPULAN) (AHLI KUMPULAN) (AHLI KUMPULAN) (AHLI KUMPULAN)

Objectives of “THE SCIENTISTS”








1. To prevent the spread of COVID-19 pandemic at Kolej



Matrikulasi Negeri Sembilan (KMNS).





2. To save cost of buying hand sanitiser by preparing DIY hand



sanitiser to be used within KMNS.





3. To begin crowd-funding for the continuous preparation of hand


sanitiser.





4. To maintain a self-sustaining hand sanitiser project.





5. To contribute towards the wellbeing of KMNS students.

Composition of Hand Sanitiser by KMNS







Ethanol (CH CH OH)
2
3
is effective in killing microorganisms like bacteria,

fungi and viruses; a common ingredient in many


hand sanitisers.



Hydrogen peroxide (H O )
2
2
is a mild antiseptic used on the skin to prevent


infection of minor cuts, scrapes and burns.



Glycerin@ Glycerol (HOCH CH (OH)CH OH)
2
2
2
is a colourless and odourless liquid that comes from

plant sources; provides protection against skin

irritants and as moisturiser.





Essential oil (EO)

Provides fragrance.

Alcohol Test on Hand Sanitiser by KMNS



























Alcohol content



79.70 % (±80%)



















Measuring the percentage of alcohol


using a Hydrometer

Production Cost of Hand Sanitiser by KMNS






To make 1.0 L hand sanitiser (20 × 50 mL bottle);




Material Cost


Ethanol 95% RM 20.80

Glycerine RM 0.35


H O 30% RM 0.10
2
2
Spray bottle (50 mL × 20 pcs) RM 12.00

Label sticker (20 pcs) RM 11.00


TOTAL COST RM 44.25



Cost to make 1 × 50 mL bottle = RM 44.25 / 20 bottle



= RM 2.20





Proposed selling price of 1 bottle is RM 5.00,


therefore each bottle gets revenue of RM 2.80!

PROCEDURE : VIRUS & BACTERIAL TEST








The bacteria and
Sources of bacteria
viruses will be
and virus are transferred directly

obtained from
from the hand to
contact with toilet
the nutritious agar
door knobs, to observe its

banknotes, toilet
growth.
faucets and the like.







Step 1 Step 2 Step 3 Step 4 The same

procedure is
Virus and Bacterial performed by

testing involving 3 Hand using different
types of hand sanitiser is hand sanitiser.

sanitisers: sprayed on

1) KMNS the hand

2) Safercare before the
3) Dettol virus was

transmitted.

Comparison of bacterial colonies exposed to hand sanitiser


made by Dettol and KMNS












































Dettol KMNS




Both hand sanitisers show similar growth rate of bacterial colonies.

st
MOST OF THE BACTERIA COLONIES of 1 BATCH HAND SANITISER HAVE STOPPED
nd
GROWING AFTER IT WAS EXPOSED TO THE 2 BATCH OF HAND SANITISER ( KMNS)










1-


2 1-
1 2




1






3
4 6


5 3
4
6
5









st
THE BACTERIA WERE EXPOSED TO 1 batch
st
THE BACTERIA COLONIES of 1 batch HAND SANITISER WERE
HAND SANITISER (KMNS) AFTER 3 DAYS
nd
EXPOSED TO 2 batch HAND SANITISER (KMNS) AFTER 3 DAYS

AFTER 3 DAYS THE BACTERIA WERE EXPOSED TO HAND SANITIZER (KMNS)



nd
2 Batch is more effective (less colonies of bacterial)










































BATCH: 1ST BATCH: 2nd

PETRI HAND SANITISER OBSERVATION (BASED ON NUMBER AND SIZE OF COLONY)


DISH DAY 1 DAY 2 DAY 3


A CONTROL 1 (WITHOUT) RAPID GROWTH INCREASED GROWTH GROWTH CONTINUES


B CONTROL 2 RAPID GROWTH + KMNS HAND SANITISER SLOW GROWTH



C KMNS SLOW GROWTH SLOW GROWTH INCREASE CONTINOUS SLOW GROWTH


D SAFERCARE SLOW GROWTH SLOW GROWTH INCREASE CONTINOUS SLOW GROWTH



E DETTOL SLOW GROWTH SLOW GROWTH INCREASE CONTINOUS SLOW GROWTH




DISCUSSION • The white spots on nutrient agar are bacterial colonies.

• Bacteria thrive in petri dish A and B compared to petri dish C, D and E on the first day.

• Rapid bacterial growth occurs on petri dish A on day 2 and 3.
• Bacterial growth was disrupted and slowed on petri dish B after being exposed to HS KMNS on the

second day.

• Bacterial growth persists on petri dishes C, D and E but at a very slow rate.
• The slowest bacterial growth is on petri dish E followed by petri dish C and D.



CONCLUSION The production of KMNS hand sanitiser has been proven through viral and bacterial testing.
Based on the alcohol test, the percentage of alcohol content in KMNS hand sanitiser exceeds 75%.

This is in line with the WHO-recommended formulation.

In conclusion, the use of KMNS hand sanitiser is safe and effective.

Acknowledgment for Hand Sanitiser by KMNS







Formulation by As recommended by Guided by



























DR. MOHD. HAZWAN BIN HUSSIN

Pensyarah Kanan Kimia Industri

&

Alumni Matrikulasi






EN. MOHAMAD SALLEH BIN AHMAD

Pengarah

Kolej Matrikulasi Negeri Sembilan