12th UeCHS UNISEL Colloquium 2022 Program Book

ORGANISED BY:

STUDENTS OF MEDICAL LABORATORY SCIENCES (HONS) & PROGRAM
BACHELOR OCCUPATIONAL SAFETY HEALTH (HONS) PROGRAM

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ORGANISED BY:
Scientific Communication (MLS 3243) students

(Semester November, 2021) from
Bachelor in Medical Laboratory Science (Hons) and
Bachelor of Occupational Safety and Health (Hons),

Universiti Selangor.

PROGRAM SCHEDULE

12TH UNDERGRADUATE E- COLLOQUIUM ON HEALTH SCIENCES 2022

TIME ACTIVITIES
8.30 am
8.45 am Online registration for poster presenters

9.00 am - Online registration for Internal Research Poster Evaluators — Lecturers from the
10.00 am MLS and OSH programmes

Opening ceremony “12th UeCHS 2022”:
▪ Online arrival of the honoree
▪ Singing of National Anthem, Selangor Anthem and UNISELsong
▪ Do’a recitation by Muhammad Shahir bin Mohd Redzuan
▪ Welcoming speech by the Director of Programme: Muhammad Nadzwan
Aizad Bin Ismail
▪ Launching of 12th UCHS E-Colloquium 2022:
▪ Live video session of speech and greeting by; Prof. Dato’ Dr. Mohammad
Redzuan Othman (President & Vice Chancellor UNISEL)
▪ Montage Multimedia presentation by FYP poster presenter 12th UeCHS

10.00 am – ▪ Final Year Research Presentation (FYP) by Student representative of group
10.20 am 1, Bachelor of Medical Laboratory Science (Hons) program.
Name : Nurul Ashikin bt Mohamed Shahrehan
10.20 am – Research title : Characterization Of Mouse Models for The Study of
10.40 am
Met Metastasis in Nasopharyngeal Carcinoma (NPC)
11.00 am Supervisor : Madam Nurkhairul Bariyah bt Baharun

12.30 pm ▪ Q & A session (5 minutes)
2.30 pm
5.00 pm ▪ Final Year Research Presentation (FYP) by Student representative of group
2, Bachelor of Occupational Safety Health (Hons) program.
Name : Nur Feriza Bt Mohamad Fozi
Research title : The Relationship Between Work-Family Conflict and Stress

Lev Among Primary School Teachers During Work from Home at A Primary
zzzzSchool In Klang, Selangor.

Supervisor : Madam Erna Haryani Amaludin
Co – supervisor : Madam Noormaliza Hamdan
▪ Q & A session (5 minutes)

▪ Video Presentation from committee 12th UeCHS 2022
▪ Online quiz competition by committee 12th UeCHS 2022
▪ FYP Poster Evaluation Session Part 1 by:
▪ Internal Research Poster Evaluators Lecturers from MLS and OSH

programs.
Break and Lunch

▪ FYP Poster Evaluation Session Part 1 by:
▪ Internal Research Poster Evaluators Lecturers from MLS and OSH programs.

End of program

WELCOME

SPEECH

MESSAGE FROM

DEAN OF
FACULTY

12TH UNDERGRADUATE E- COLLOQUIUM
ON HEALTH SCIENCES 2022

Assalamualaikum W.B.T and Greetings to All.
Alhamdulillah, all thanks to Allah S.W.T with His mercy and grace, the 12th Undergraduate e-Colloquium on
Health Sciences (12th UeCHS) held today. Congratulations to registered students' Scientific Communication
semester November 2021 and the Faculty of Health Sciences on organizing this significant programme.
Due to the current COVID-19 pandemic, this is the fourth time for the e- Colloquium on Health Sciences
ceremony to be held online instead of face-to-face. Despite this challenging condition, we still have over 500
guests and students joining our Ceremony online by FB live.
Today's world has evolved from employing human resources to utilizing automation and technology. Human
capital is still considered important in our effort to develop a strong background in science and technology
and should be given extra attention, particularly at the workplace. Thus, the occupational safety and health
field is vital to ensuring the organization's safe and efficient operation.
Today's program should be used preferably by sharing expertise and knowledge of science and looking for
alternatives to enhance this field through continuous research. The program is also able to be the best
platform for students and lecturers to present their research findings to improve the quality of healthcare
through scientific research carried out, in line with this program tagline, which is "A true devotion to a new
finding".
Finally, I hope that today's programme will provide as enormous exposure as possible to all the lecturers and
students of Health Sciences of UNISEL, eventually contributing to the development of the university, state
and country.

MR MOHD SHARIKH DALI BIN MAHAD
Dean
Faculty of Health Sciences
Universiti Selangor

MESSAGE FROM

THE PROGRAMME
ADVISOR

12TH UNDERGRADUATE E- COLLOQUIUM
ON HEALTH SCIENCES 2022

Assalamualaikum W.B.T and Greetings to All.
All praise is due to Allah, Faculty of Health Sciences (FHS) once again is organizing the “12th Undergraduate
Colloquium on Health Sciences” with the theme “A True Devotion To A New Finding”
I, on behalf of the Organizing Committee of 12th UCHS would like to humbly welcome all participations,
internal and external examiners to this important colloquium. This colloquium is aiming to provide a platform for
Final year students’ projects and research committee members to present their findings as well as share valuable
experiences and knowledge to further enrich the field of Health Sciences.
The 12th Undergraduate Colloquium on Health Sciences provided opportunity to oral presenters to present
cutting-edge work and significant challenges currently being addressed in the Health Sciences research. Through
12th UCHS also invited external poster evaluators together for evaluate our final year project findings.
The success of this colloquium depends on the tireless efforts of committee members. Therefore, on behalf of
the management committee, we would like to extend our heartfelt gratitude to Universitimanagement
committee, all sponsors, lecturers and students and all parties who have been involved directly or indirectly in
this 12th UCHS 2022. In addition, I would like to take this opportunity to thank the oral presenters, internal
poster evaluators, and poster presenters for generously sharing their knowledge and research discoveries.
I sincerely hope that this colloquium will be beneficial to all participants and other interested readers
scientifically and they are encouraged to continue striving to reach excellence in Health Sciences research in
the process. Finally, I would like to wish best of luck to all poster presenters in their research project. Once again,
congratulations and well done to the organizers who have worked hard to make this 12th UCHS successful.

With warmest regards,

DR. YUSMI BIN MOHD YUNUS
Program Advisor
12th Undergraduate Colloquium on Health Sciences
Faculty of Health Sciences
Universiti Selangor

MESSAGE FROM

THE PROGRAMME
DIRECTOR

12TH UNDERGRADUATE E- COLLOQUIUM
ON HEALTH SCIENCES 2022

Assalamualaikum W.B.T and good day everyone.
First of all, I would like to welcome our honourable guest, Y. Bhg. Prof. Dr. Md Sidin Bin Ahmad Ishak Vice
President & Deputy Vice Chancellor (Academic, Research and Industrial Engagement). To our respected
Dean Faculty of Health Sciences, Mr. Mohd Sharikh Dali bin Mahad, The Deputy Dean, Student Development
and Resources, Ts. Sawal bin Marsait. The Deputy Dean Academic, Madam Izreen binti Supa’at, Dr. Yusmi bin
Mohd Yunus, beloved lecturers, staffs and to all of you.
Alhamdulillah, even with the movement control order, we are able to participate virtually for the inaugural
ceremony of the 12th Undergraduate e-Colloquium on Health Sciences. I am delighted and pleased by the
honored guest, lecturers, and employees of the Faculty of Health Sciences, as well as those who follow us live
on our official page. It’s a fantastic experience.
Every year, we hosted the UCHS for undergraduate students in their last year of study in the Scientific
Communication subject. This colloquium allows us to create a polished piece of original work based on our
scientific knowledge, oral communication, information literacy, critical thinking, organisational abilities, and
much more.
The 12th UeCHS tagline is “A TRUE DEVOTION TO A NEW FINDING,” which emphasises that devotion
denotes a commitment or dedication to any objective in which all members work together hand in hand to
make this endeavour a success. Dedication and teamwork aid in the discovery of new experiences, knowledge,
and memories. It is also the result of enthusiastic and devoted research aimed at providing constant scientific
breakthroughs, such as new model analysis and unlocking new strategies to battle disease for a better future.
Nevertheless, our team, members of the 12th UeCHS Committee, and I would like to apologise for any flaws or
limitations that occurred during the event. We hope everyone had a good time and hope to see everyone again
soon. Hopefully. Take precautions and stay safe, as the number of COVID-19 cases continues to rise and new
variants emerge. Have a wonderful day.

Thank you.

MR MUHAMMAD NADZWAN AIZAD BIN ISMAIL
Programme Director
12th Undergraduate Colloquium on Health Sciences
Faculty of Health Sciences
Universiti Selangor

LIGHT BLUE

Symbol of communication, which is the primary goal of UCHS: to disseminate new scientific
discoveries. Blue also represents the brain, which is striving to come up with ideas to make this
colloquium operate as planned.

PINK

Symbol of optimism and hope. It also symbolises the DNA cycle

PINK

The theme colour Orange has a crucial connotation in that it represents “We are Uniselian,”
indicating that it is the Unisel University’s Universal colour. The colour orange also represents the
bright future we may have as Uniselians.

BRAIN DNA CIRCLE

The brain reflects as a sign of Surround the brain: depicts Symbolizes the circle of
a “cip,” which has the highest the ongoing research that will the scientific community
potential of storing a ton of continue until the end for the that serves as a source of
information. benefit of all. knowledge to society.

OBJECTIVE

Sharing skills and Exchange of ideas
knowledges in and fundamental
concepts of health
current scientific
research science

TA G L I N E

“ATRUE DEVOTION TO A NEW FINDING”

Devotion means a commitment or dedication to some purpose where all the member
are working together hand by hand to make this project a success. The dedication
and teamwork help to find a new experience, knowledge and memory together. It is
also the passionate and committed research aiming to provide continuous scientific

discoveries including the new model analysis and unique new ways to fight disease for
a better future.

N ATIONAL Negaraku
ANTHEM Tanah tumpahnya darahku,
Rakyat hidup
bersatu dan maju,

Rahmat bahagia
Tuhan kurniakan,
Raja kita
selamat bertakhta,

Rahmat bahagia
Tuhan kurniakan,
Raja kita
selamat bertakhta.

Duli Yang Maha Mulia SELANGOR
Selamat di atas takhta ANTHEM

Allah lanjutkan usia
Tuanku Rakyat mohon restu

Bawah Duli Tuanku
Bahagia selama-lamanya

Aman dan sentosa
Duli Yang Maha Mulia

UNISEL Disini kemahiran jadi budaya
ANTHEM Kepakaran sendi kerjaya
Ilmu dihayati sepanjang usia
Amal jariah tunjang jasa
Dipayungi langit tanpa sempadan
Bumi Subur Darul Ehsan
Disinari suria alam Tuhan Yang Esa
Dibuai bayu ilmu peradaban

UNISEL…

Kaulah sinar bestari
Pancaran ilmu serta amali
Pengisi minda pengasuh budi
Pelopor utama insan mithali
Kau umpama pohon nan rimbun
Tumbuh subur membawa tamadun
Jati diri hidup berukun
Tahan uji turun menurun

UNISEL…

Kaulah sinar bestari
Pancaran ilmu serta amali
Pengisi minda pengasuh budi
Pelopor utama insan mithali

ORAL PRESENTERS

ABSTRACT

C H ARAC TERI Z ATI O N O F M O U S E
MODELS FOR THE STUDY OF
METAS TAS IS IN N A S O P H A RY N G E A L
CARCINOMA (NPC)

Nurul Ashikin Mohamed Shahrehan1,2, Nurkhairul Bariyah Baharun1

1Faculty of Health Sciences, Universiti Selangor
2Molecular Pathology Unit, Institute for Medical Research

ABSTRACT

The burden of disease from NPC is disproportionately large for Malaysia compared to the world. In Malaysia
the data show a very high incidence and mortality. Metastasis is a highly complicated biological phenomena
that continues to be a clinical concern in the treatment and management of NPC. Over the years, several
attempts have been made to construct a mimetic model for the study of metastasis. A metastatic model
system for cancer is an important research tool that may be adjusted to look at different elements of
NPC. Cancer models based on mice are well-known and regularly utilized in cancer research. For a better
knowledge and medication development in this condition, clinically appropriate mice models are required.
In this study, Nod Scid Gamma Null was chosen as model and two (2) nasopharyngeal carcinoma cell lines,
stably expressing green fluorescent protein (GFP), Xeno C666-GFP and Xeno B110-GFP were
orthotopically injected into the nasopharynx of the mice. After the injection, the whole-body fluorescence
imaging was used to monitor the growth of the primary tumor. As the result, the in vivo and ex vivo imaging
shows the metastasis to the mice organs and hematoxylin and eosin (H&E) staining was done to verify
the tumor region which conclude that the metastasis can exist by tumor cells spread naturally from the
primary tumor site to secondary locations the when the NPC cells was orthotopically injected to the mice
models. With this result, the imageable orthotopic model should be useful in explaining the processes
behind nasopharyngeal cancer development, progression and metastasis, as well as evaluating new drugs
with potential effectiveness

KEYWORDS: Nasopharyngeal carcinoma, metastasis, mouse model, imaging

THE RELATI O N S H I P B E T W E E N
W O R K - FA M I LY C O N F L I C T S A N D
STRESS LEVEL AMONG PRIMARY
S C H O O L T E A C H E R S AT A S C H O O L IN
KLANG, SELANGOR

Nur Feriza Mohamad Fozi, Erna Haryani Amaludin, Noormaliza Hamdan

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

Work and family conflict arises when an individual’s job and family responsibilities become
conflicting. The conflict must be handled with caution because they are linked to bad outcomes
especially among primary school teachers during working from home. The aim of this study is
to determine the relationship between work-family conflict and stress level among primary
school teachers during work from home. This study has taken placed at a primary school in Klang,
Selangor. The study applied Work and Family Conflict Scale (WAFCS) and Perceived Stress Scale
(PSS) to the 36 respondents of primary school teachers. The result had showed that there is no
significant relationship between work and family conflict (work-family conflict, p=0.166) with
stress level. In conclusion, the hypothesis for relationship between work-family conflict and
stress level among primary school teachers was rejected.

KEYWORDS: work-family conflict, stress, work from home, work and Family Conflict Scale (WAFCS),
Perceiver Stress Scale (PSS-10)

POSTER PRESENTERS

ABSTRACT

RAPI D AUTO MATED BAC TERI AL
I D EN TI F I C ATI O N TES TI N G A N D
ANTIBIOTICS SUSCEPTIBILITY
T E S T I N G D IREC T LY F R O M P O S I T I V E
B L O O D C ULTUR E

Angelyn Ann Yeo1,2, Norhatiah Md Lias1

1 Faculty of Health Sciences, Universiti Selangor
2 Hospital Pulau Pinang

ABSTRACT

Manual bacteria identification method required 24 hours incubation in order to identify the
bacteria and antibiotics sensitivity testing using standard disc diffusion method required 24 hours
of incubation. The purpose of this study are to compare bacteria identification by using manual
identification method, Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF)
mass spectrometry (MS) and IVD Sepsityper kits and to compare the significance of the antibiotics
sensitivity testing from the positive blood culture between standard disc diffusion and Rapid
Antibiotics Sensitivity Testing (RAST). 100 aerobic blood culture bottles which flagged positive are
cultured and identified by using 3 different method; manual identification method, MALDI- TOF
and IVD Sepsityper kits. At the same time, antibiotics sensitivity testing will be performed on the
organisms isolated by using 24 hours standard disc diffusion method and 8 hours RAST method.
As a result, there is no significant difference between the bacteria identification between manual
identification and MALDI-TOF but there is a significant difference when using IVD Sepsityper
kits. There is no significance difference for the antibiotics susceptibility testing between standard
disc diffusion and RAST for gram positive cocci in cluster but there is a significance difference for
gram positive cocci in chain and gram negative rod. In conclusion, manual identification method
and MALDI-TOF have the same ability and performance to identify the same pathogen whereas
IVD Sepsityper kit unable to identify some pathogens. Antibiotics susceptibility testing for gram
positive cocci in cluster have the similar interpretation but for gram positive cocci in chain and
gram negative rod have different interpretation between standard disc diffusion and RAST.

KEYWORDS: bloodstream infections, matrix-assisted laser , Desorption/Ionization–Time of Flight Mass
Spectrometry (MALDI-TOF MS), IVD Sepsityper Kit, antibiotics susceptibility testing, agar disc diffusion,
Rapid Antibiotics Sensitivity Testing (RAST)

C O RRELATI O N O F FERRITIN L E V E L
AND IRON PROFILE AMONG LOW
HEMOGLOBIN PREGNANT MOTHER
IN TA N G K A K PO PULATI O N

Aqilah Nabilah Suliman1,2, Asma Harun1, Rahidatul Fairuz Ibrahim3

1Faculty of Health Sciences, Universiti Selangor
2Pejabat Kesihatan Tangkak
3Hospital Pakar Sultanah Fatimah, Muar.

ABSTRACT

Iron deficiency is the most prevalent single nutritional deficiency, affecting approximately 20% of
the world’s population (William, 2000). The purpose of this study is to identify correlation of iron
storage based on Serum Ferritin level and Iron profile among low hemoglobin pregnant mother
attending Klinik Kesihatan in Tangkak between 2019 until 2021. A cross-sectional study was
done over a two-year period and 90 pregnant mother were enrolled in this study. Low hemoglobin
pregnant mother is identified and later tested with serum ferritin and iron profile as a screening
tool for detecting absolute iron deficiency anemia. Blood sample was drawn from apparently low
hemoglobin pregnant mothers at antenatal booking and sent for laboratory assessment serum
ferritin and iron profile (serum iron, UIBC, TIBC and transferrin saturation. The results showed
that 65 out of 90 participant (72.2%) was absolute Iron deficiency Anemia (IDA) only by referring
serum ferritin. Approximately 76% are Malay followed with Indian and Chinese came to Klinik
Kesihatan were diagnosed as IDA as they have low Hb. The serum ferritin level was significantly
associated with the Iron Profile (p value = <0.001). Serum ferritin is positive correlation with
serum iron and transferrin saturation. Hence if Serum ferritin decrease, serum iron and transferrin
saturation also decrease. However, Serum ferritin with UIBC and TIBC are negative correlate
where when Serum ferritin increase, UIBC and TIBC will decrease. It is important to detect iron
deficient erythropoiesis during the first antenatal check-up, as it is an early manifestation of iron
deficiency anemia. In conclusion, screening for iron deficient using serum ferritin alone is reliable
and cost efficient rather than sending both serum ferritin and iron profile in diagnosing IDA
among low Hb pregnant mother.

KEYWORDS: ferritin, iron profile, iron deficiency anemia , low hemogblobin pregnant mother

A N A LY S I S O F V I TA M I N D IN
METABO LIC S Y N D R O M E USING
I M M U N O A S S AY METH O D

Asvini Manikian1,2, Yusmi Mohd Yunus1

1 Faculty of Health Sciences, Universiti Selangor
2 Hospital Putrajaya

ABSTRACT

Vitamin D is a fat-soluble vitamin that is usually found in two forms, which are known as
vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). vitamin D can be easily obtained
from sunlight, but certain environmental conditions, genetic factors and lifestyle can influence
this. Factors that affects the levels of vitamin D are including sun exposure, age, race, adiposity,
metabolic syndromes and drug usage. The aim of this study is to measure the level of vitamin D
deficiency in metabolic disorder among adult peoples in Peninsular and West. Vitamin D test
run by using UniCel DxI 800. Access 25(OH) Vitamin D Total assay uses a competitive two-step,
binding immune-enzymatic assay process. In this study, there was a total of 370 patients that has
been diagnose with Hypertension, Diabetes Mellitus and obesity. In the total of 370 patients,
there were 114 male patients and 256 female patients. There are 67 males (18.11%) and 148
female (40%) selected from East Malaysia while 47 males (12.70%) and 108 females (29.19%) from
West Malaysia. From this study, it shows there is no statistically significant difference between
metabolic group syndrome group and the vitamin D level test (p&gt;0.05). This study did not
provide conclusive evidence that vitamin D deficiency is associated with metabolic syndrome,
due to limited sample size obtained amidst Covid-19 pandemic.

KEYWORDS: Vitamin D, hypertension, diabetes mellitus, obesity, metabolic syndrome, UniCel DxI 800

DETECTION OF TICK-BORNE
PAT H O G E N ( R I C K E T T S I A S P P ) IN
TICKS USING NESTED PCR A S S AY

Canedy Jacob ,1,2 Ahmad Norasidi Mohd Raffie1 , Foo Phiaw Chong2,
Ernieenor Faraliana Che Lah2, Mariana Ahamad2
1 Faculty of Health Sciences, Universiti Selangor
2 Institute for Medical Research

ABSTRACT

Tick-borne rickettsiae is a Gram-negative obligate intracellular bacteria belonging to the
spotted fever group (SFG) under the genus Rickettsia, which many of them possessed
significant medical importance that causes febrile diseases. Rickettsioses have been
documented in Southeast Asian countries and identified as emerging infections in Peninsular
Malaysia. The aim of this study is to identify the presence of Rickettsia spp. in ticks collected
from Pahang and Terengganu. A total of 184 archived tick's samples collected from forests of
Pahang and Terengganu from 2018 until 2019 were identified and proceeded for detection of
Rickettsia spp. using nested PCR. Two specific primer sets were designed targeting the ompA
gene and 17-kDa gene for the detection of Rickettsia bacteria, focusing on the spotted fever
group. A validated dual-target one-step Nested PCR assay was used to detect the presence of
Rickettsia spp. in the extracted DNAs. In the 184 samples, 67 (36.4%) samples were collected
from Pahang locality while 117 (63.6%) samples were from Terengganu locality. Among the
184 samples tested, only one (0.5%) sample was detected positive for Rickettsia spp based on
the 17-kDa gene. The tick was identified as Ixodes granulatus and was collected from Pahang.
Sequence alignment obtained from the sequencing showed 100% identical to one of the
Rickettsia spp. gene found in NCBI GenBank. The result suggested that the positively amplified
sequence belonged to Rickettsia sp. ATT 17-kDa gene.
Keywords: Tick-borne pathogens, Spotted Fever Group Rickettsia (SFGR), Rickettsioses, Dual-
target one-step Nested PCR Assay.

USE OF IN VIVO MODEL
FOR RESEARCH IN
D E V E L O P M E N TA LT H E R A P E U T I C S O F
NASOPHARYNGEAL CARCINOMA

Christine Ricky1,2, Nurkhairul Bariyah Baharun1

1 Faculty of Health Sciences, Universiti Selangor
2 Molecular Pathology Unit, Institute for Medical Research

ABSTRACT

Nasopharyngeal carcinoma (NPC) is a cancer that has a distinct biology and clinical features,
highly associated with Epstein-Barr virus (EBV) infection. As the cancer is rare in the West, there
are very limited new therapeutic agents developed for this cancer. Before use in humans, drugs
need to be tested in pre-clinical models, hence xenograft models are used as models for drug
testing. The aims of this study were to compare the effect of carfilzomib on tumor volume and
body weight both treatment and control group in Nod Scid Gamma (NSG) mice. PDX B110 were
inoculated subcutaneously into the right lower flank of mice, monitored and measure the tumor
size until it reached 80 - 120 mm 3 . The mice were randomizing into three groups, two treatment
and a control both with different regime. Treatment was delivered through intraperitoneal (IP)
injections until week three. Throughout the weeks, tumor measurement was done thrice a week
and body weight were measured daily. Tumor were harvested when it reaches humane end point.
Data were then analyzed, showed no significant effect of carfilzomib against B110. There was
some reduction for both treatment groups but when compared to control, the reduction was not
statistically significant (p&gt;0.05). The use of carfilzomib as a treatment option in PDX B110
can be achieved if larger sample size with a lower passage number of PDX B110 were used, and
treatment prolonged until week four.

KEYWORDS: Nasopharyngeal carcinoma, drugs, treatment, tumor

E VA L U AT I O N O F H E A LT H Y
LIFESTYLE PROGRAM ON STUDENT’S
H E A LT H STATUS IN I L K K M K U A L A
LUMPUR

Edmond Ubil Zulkifli1,2, Sharifah Izwan Tuan Othman1

1Faculty of Health Sciences, Universiti Selangor.
2Institut Latihan Kementerian Kesihatan Malaysia (ILKKM)
Kuala Lumpur.

ABSTRACT

Healthy Lifestyle Program (HLP) is conducted in ILKKM Kuala Lumpur to help students maintain
and improve their overall health status as they are seen to be gaining weight throughout their
three years of training. About 20% are overweight, and after several years into working life, they
become obese and starts having health problems mostly related to cardiovascular diseases (CVD).
A total of 123 students included in the study run for 3 months that includes 10000 steps activity,
healthy diet challenge, and healthy lifestyle awareness including mental health, emotional health,
and general health such as healthy sleep and learning about food nutrition. Their body mass
index (BMI), waist to hip (WTH) ratio, and body fat percentage (BF%) measurements as well as
blood samples for total cholesterol (TC), and fasting blood sugar (FBS) level were recorded pre-
program and then recorded again on post-program. Student’s parameters data differences
between pre-program and post-program were calculated using paired samples t-test method to
determine the effectiveness of the program. Out of 123 students, results after 3 months HLP
shows 29.3% increase of normal BMI (92.7% vs 63.4%), 6.5% improvement of low risk WTH ratio
(33.3% vs 26.8%), 5.7% better BF% (93.5% vs 87.8%), excellent in both normal TC level by 38.2%
(100% vs 61.8%) and FBS level by 8.9% (100% vs 91.1%). The mean differences of all parameters
between pre-program and post-program are also highly significant at p < 0.001. Therefore, the
study validates that the HLP conducted for 3 months is effective on improving the overall health
status of ILKKM Kuala Lumpur’s students. However, further study focusing on targeted sample
population such as age, ethnicity, and socioeconomic variables for more accurate validation on
the program’s effectiveness across all demographics.

KEYWORDS: healthy lifestyle, obesity, cardiovascular diseases, intervention, effectiveness, evaluation,
body mass index, total cholesterol, fasting blood sugar.

A N A LY S I S O F E S T I M AT E D
G L O M E R U L A R FILTRATION R AT E
A M O N G H Y P E RT E N S I V E PATIENTS
F O R E A R LY D E T E C T I O N O F K I D N E Y
FAI L U R E AT K L I N I K K E S I H ATA N
KULAI BESAR

Indriani Indar1,2 , Asma harun¹, Hamidah Abu Bakar1

1 Faculty of Health Sciences, Universiti Selangor
2 Klinik Kesihatan Kulai Besar, Johor

ABSTRACT

Renal failure is a global public health issue that frequently leads to kidney failure and end-stage
renal disease. Adults who have hypertension, diabetes, or both are more likely to develop chronic
kidney disease (CKD) than those who do not. Hypertension has been linked to a faster progression
of CKD. Many studies have shown that treating hypertension patients with CKD can slow the
rate of glomerular filtration rate decline (GFR). This study was conducted to evaluate creatinine
level as early detection in changes of glomerular filtration rate among hypertensive patients.
The creatinine level was measured using Jaffe’s method in biochemistry analyser. The repeated
ANOVA calculation method was used to measures the glomerular filtration rate parameter. This
study was an analytical study that measures the calculation of eGFR patients for 3- year
reading. Samples (n=140) were collected in tubes of a plain tube. The plasma is analysed using
biochemistry analyser, Beckman Coulter AU 480. Data were collected and analysed using SPSS
version 25. As a result, no statistically significant (p>0.112) between the year 2019 to 2021
measurement. In conclusion, the value of eGFR in serum creatinine of hypertensive patients has
no significant difference between 3 years, thus making no necessity to send for further test such
as cystatin C, to detect early kidney disease. This allows medical practitioners to only monitor the
eGFR value in serum creatinine.

KEYWORDS: creatinine,estimated glomerular filtration rate,hypertension,kidney failure

PREVA L E NC E O F D ENGUE C A S E S
DURING RESTRICTION MOVEMENT
O R D E R AT K L I N I K K E S I H ATA N M A S A I

Ismadi Surry Lisius 1,2 , Izreen Supa’at1

1 Faculty of Health Sciences , Universiti Selangor
2 Klinik Kesihatan Masai

ABSTRACT

Prevalent cases of dengue fever are on the rise during movement-controlled order (MCO).
Dengue causes a substantial burden to the patients not only physical pain but also economic
hardship to them and their family. Due to the spread of Covid19, not only health workers but
all sections of society are more focused on breaking this chain than continuing to rise. This is
because the symptoms reported have similarities with Covid19. This causes the investigation to
identify dengue to be slow compared to the investigation for Covid19. This study was conducted
to analyse the trend of dengue cases during the MCO from March until June 2019 ( before MCO )
and March until June 2020 ( during MCO period). Specifically, this study was conducted to analyse
the demographic data of dengue patients, to measure the frequency of dengue cases from March
until June 2019 & March until June 2020 and also to compare the frequency of dengue cases in
2019 and 2020. A total of 345 of dengue patient were involved in this study mainly lives in the
district of Masai Health Clinic in Johor Bahru. The research design was quantitative, descriptive
design and non - experimental. Method used is non - probability of convenience sampling. The
instrument used is a census from laboratory of Masai Health Clinic. The data done analyse using
the Statistic Package for Sosial Science (SPSS) version 21.0. The statistical test used in this study
is frequency test. The result show that increasing in total cases of dengue fever during restriction
movement order for two years in March until June 2019 and 2020. Therefore, collective and
integrated efforts by all parties are essential to ensure the success of dengue prevention on an
ongoing basis. In fact, if we are concerned, various methods can be done to prevent the spread
of dengue.

KEYWORDS: dengue fever , dengue cases trend , restriction , frequency dengue cases

THE AS S O C I ATI O N O F HbA1C A N D
T H Y R O I D H O R M O N E STATUS IN T Y P E
2 D I A B E T E S M E L L I T U S AT H O S P I TA L
RAJA PERMAISURI BAINUN, IPOH

Kishaanthan Balakrishnan1,2, Asma Harun1

1Faculty of Health Sciences, Universiti Selangor
2 Hospital Raja Perempuan Bainun

ABSTRACT

Diabetes Mellitus (DM) is a multi-factorial disorder. Mainly Type 2 Diabetes mellitus (T2DM)
considered by hyperglycaemia which results commencing from defective insulin secretion or
insulin action or both due to social influence and changes in lifestyle. Patients with DM are at
increased risk of thyroid diseases especially those with poor glycaemic control. Various studies
have indicated that T2DM may influence Thyroid Dysfunctions (TD). The prevalence and degree
of various thyroid dysfunction between T2DM can be assess by correlating these two tests which
is HbA1c and Thyroid Function Test (TFT) at Hospital Raja Permaisuri Bainun, Ipoh. T2DM was
detected by identifying each peak, plus the relative percent and retention times of each peak
utilizes the principles of ion exchange high performance liquid chromatography (HPLC). TFT is
includes Thyroid Stimulating Hormone (TSH), Free T4, and Free T3. The TFT test was analysed
using electrochemiluminescence immunoassay (ECLIA) method and d the results were classified
into three distinct level such as low, normal and high. A total of 277 out of 384 patients; 145 males
and 132 females were diagnosed with T2DM. For patients with T2DM, 268 patients show normal
thyroid function, 7 patients show possible sign of hyperthyroidism and there are 2 patients have
sign of hypothyroidism. Furthermore, The Chi-square test was used to determine the relationship
between diabetes and normal group. This study did not provide conclusive evidence that HbA1c
had associated with thyroid dysfunction in T2DM patients in Hospital Raja Permaisuri Bainun,
Ipoh, due to lack of resources among the physicians about the correlation between HbA1C and
thyroid functions test to monitor patients with T2DM. However, supportive findings have shown
that majority T2DM patients has thyroid dysfunction

KEYWORDS: Diabetes Mellitus (DM), Electrochemiluminescence Immunoassay (ECLIA), High Performance
Liquid Chromatography (HPLC), Type 2 Diabetes mellitus (T2DM), Thyroid Dysfunctions (TD), Thyroid
Function Test (TFT), Thyroid Stimulating Hormone (TSH)

DENGUE FEVER TREND OF DENGUE
INFECTION IN GOVERNMENT HIGHER
EDUCATION C E N T E R

Muhamad Nazri Md Nasir1,2, Yusmi Mohd Yunus1 , Hairani Mohd Khalid2

1 Faculty of Health Sciences, Universiti Selangor
2 International Islamic Universiti Malaysia Health And Wellness Center
(IIUM HWC)

ABSTRACT

Dengue infection is a life-threatening in our country hence need better understanding for
prevention and management. NS1 dengue test and combo immunoglobulin G (IgG) and
immunoglobulin M (IgM) were analysed. NS1 is detectable during acute phase whereas IgG and
IgM are primarily used to help diagnose a current or recent infection of dengue fever infection.
The dengue trend infection in government higher education centre in Gombak was measured
and the trend was analysed. A total of 351 patients’ samples from January 2020 to April 2021
were taken and analysed. There were 7,4,3,5 and 8 positive cases for NS1 in January, February,
October, November and December respectively. For IgG and IgM, there were 5,11,2,3 and 5 cases
positive in January, February, October, November and December respectively. The aim of this
research is for better understanding of an effective engagement strategy of dengue prevention
and increase public awareness about life-threatening of dengue to human. The trend of dengue
infection in this study is unique. This prevalence study did not show a clear cut about the trend
of dengue infection. However, this trend of dengue infection is necessary and useful to increase
public awareness of how to curb the dengue cases so that less cases for dengue infection in the
future. It is also giving a significant understanding to public for better prevention, management
and treatment about dengue infection. In conclusion, Further studies should be conducted with
a larger sample size by using other method and testing with a modern technologies to carry out
an established researched study.

KEYWORDS: Dengue, Selangor, Infection, and Dengue NS1.

ANTI-BIOFILM ACTIVITY OF
LACTOBACILLUS AGAINST
ESCHERICHIA COLI
INFECTIOUS: A REVIEW

Muhamad Syamim Roslan , Norhatiah Md Lias

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

Biofilms have been described in many systems since Van Leeuwenhoek examined the
“animalcules” in the plaque on his own teeth in the seventeenth century, Biofilm formation
begins when free-floating microorganisms such as bacteria come in contact with an
appropriate surface and begin to put down roots. Biofilm are complex microbial communities
established on a wide range of a surface that are generally encapsulated by an extracellular
protective matrix compose of various types of biopolymers. EPS is called 'black matter of
biofilms. EPS can differ significantly between biofilms depending on the type of the
microorganism, the shear forces experienced, the temperature and the availability of nutrients.
EPS was originally referred to as 'extracellular polysaccharides' but was changed when it
became apparent that the matrix also includes enzymes, nucleic acids, lipids and other
biopolymers such as humic substances. Extracellular bacterial structures such as flagella, pili
and fimbriae can also stabilize the matrix. Biofilm forming Uropathogenic E. coli are associated
with persistent and chronic inflammation leading to complicated and or recurrent UTIs.
Biofilms provide an environment for poor antibiotic penetration and horizontal transfer of
virulence genes which favors the development of Multidrugresistant organisms. Used of
Lactobacillus as Anti-biofilm by used their probiotic properties can help against infectionous
disease from Escherichia coli. From the result, and L. acidophilus has stronger ability to against
Escherichia coli infection.

KEYWORDS: Bio-film, anti-biofilm, Lactobacillus, Escherichia coli

T H E H EAT S T R A I N S C O R E I N D E X
(HSSI) A M O N G STALL A N D
P E T R O L STATION W O R K E R S IN
KLANG,SELANGOR

Muhammad Ammar Abdul Rahman, Noormaliza Hamdan, Amir Arshad

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

Petrol station workers and stall workers are greatly potential to get excessive hot temperature
while working especially during the middle of the day. Thus, this study was embarked with the
objective to identify the comparative result of heat strain score index (HSSI) between petrol
station and stall workers in Klang, Selangor. An expected result was taken as data collection which
is a total of 25 sample size for each, equal to 50 total sample size was taken. A questionnaire
known as Heat Strain Score Index (HSSI) was used to determine the heat strain index among
petrol station and stall workers that was adapted from previous research for the purpose of this
study. Hence, in previous research HSSI can be used as the heat strain screening tool in the warm
workplace when other heat stress evaluation methods are not available. A chi square analysis is
being used to determine the p-value of HSSI. There was statistically significant between green
zone and yellow zone of heat strain among the workers due to the p-value is less than 0.05
(p<0.05). The findings indicate that there is significance for this study where difference of the p-
value is .006738, which (p<0.05). It can be concluded that the hypothesis is accepted, since
there is an association of heat strain score index due to p-value is less than 0.05.

KEYWORDS: petrol station, stall, comparative, Heat Strain Score Index (HSSI).

STRESS AND ANXIETY
A M O N G H E A LT H S C I E N C E S
STUDENTS,UNIVERSITI SELANGOR
DURING MOVEMENT CONTROL
ORDER

Muhammad Nadzwan Aizad Ismail, Izreen Supa'at, Noormaliza
Hamdan

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

There is a lot of news heard on social media and also television that mentioned the public mental
health of Malaysian citizen are getting more worse during Movement Control Order. By getting
the statistics on how the current mental condition of people especially young adult UNISEL
students, it might be a help for certain organizations like the Student Welfare Unit to provide
some help to those in need. This study aimed is to determine the mental status among Faculty of
Health Sciences UNISEL during Movement Control Order. Survey-based research which involved
Faculty of Health Sciences UNISEL using Google Form questionnaire to obtain demographic,
details of programme, mental health and stress status, distributed online and data analyzed in
SPSS software. The inclusion criteria of respondent must be adult male and female UNISEL
students. Able to understand Malay or English, not smoking and drinking alcohol The exclusion
criteria include students on 1st semester and undergoing internship. 75 students out of 77 were
chosen after data cleaning and mental health status of UNISEL students were classified by using
verified GHQ-12 and PSS-10 questionnaire and the outcome was about 24% of respondents are
having high perceived stress and psychological distress. By using the Pearson Chi-Square at α =
0.05 level of significance, the association of psychological distress and level of stress were resulted
statistics value 24.142 with the p-value of 0.000 which considered significant. In conclusion,
this study managed to reveal mental health status of Faculty of Health Sciences UNISEL during
Movement Control. Prolonged stress can lead to setbacks, which can hinder students’ academic
performance and increase the likelihood of mental health problems at UNISEL’s Faculty of Health
Sciences.

KEYWORDS: stress, anxiety, movement control order (MCO), General Health Questionnaire (GHQ-12),
Perceived Stress Scale (PSS-10)

E VA L U AT I O N O F D I F F E R E N T
C EN TRI F U G ATI O N S ETTI N G S O N
CLINICAL CHEMISTRY PROCEDURE,
H O S PI TAL TEN GK U A M P U A N
RAHIMAH, KLANG

Muhammad Nur Hidayat Mohd Janny1,2, Sharifah Izwan Tuan Othman1,
Hanisah Abdul Hamid2

1Faculty of Health Sciences, Universiti Selangor
2 Hospital Tunku Ampuan Rahimah

ABSTRACT

In laboratory specimen throughput, centrifugation time is a major bottleneck procedure. In
most circumstances, centrifuge samples on a swing-out rotor centrifuge for 10 minutes is
recommended. But it seem too time consuming to utilise this centrifugation setting in crowded
hospital. Alternate procedure was applied in Hospital Tengku Ampuan Rahimah Klang as
replacement to manufacture’s setting recommendation, which using shorter timing and faster
RPM, 5 minutes 4000RPM. This study conducted to compare the present of haemoglobin in
serum and result of clinical chemistry aspartate transferase (AST), creatinine kinase (CK), Total
Bilirubin (Tbil), alanine transferase (ALT) and Potassium (K+). Clinical chemistry analytes are
chosen for each level severity from slightly, medium and severe. Sample were collected from 25
volunteers with consent informed. Next, the sample take to centrifugation process with 2
setting and the serum of the sample were analysed with analyser Siemens XPT Chemistry and
Sysmex XN3100. The result were collected, validated and plotted using SPSS application. The
data in independent t-test show there is no significant different between two centrifugation
settings (P > 0.05 for all analytes). We successfully determine the reliability of the centrifugation
setting 5 minutes/ 4000 RPM compare to CLSI/ manufacture’s recommendation 10 minutes at
1500xg/ 3400 RPM. The current laboratory’s centrifugation setting maybe applied for clinical
chemistry testing. Centrifugation setting 5 minutes/ 4000 RPM produce same quality of serum
compare to 10 minutes at 1500xg/ 3400 RPM CLSI/ manufacture’ s recommendation setting.
This laboratory’s current setting should be apply in same laboratory to improve turn around time
without compromise serum quality.

KEYWORDS: Centrifugation, setting 1, setting 2, AST,Tbil,ALT,K+,CK, HB

C O M PA R I S O N O F LIPEMIC, ICTERIC
A N D H A E M O LY S I S S P E C I M E N
BETWEEN VISUAL BY THREE
O B S E RV E R S A N D AUTO MATED
DETECTION

Muhammad Shahir Mohd Redzuan1,2, Asma Harun1, Nur Aasyirah
Zakariah2

1Faculty of Health Sciences, Universiti Selangor
2Hospital Sultan Abdul Halim

ABSTRACT

Serum indices are a tool that informs laboratory personnel about potential interferences, improves
sample quality, and reduces abnormal test findings. Before the sample is analysed, this procedure
must be carried out at the pre-analytical stage. The three types of serum indices that must be
measured are lipemic, icteric, and haemolysis of the sample. All three are critical in determining
the sample’s condition before it is processed. It is well known that not all laboratories have
machines that can do automated analysis. As a result, to remedy this issue, medical laboratory
technologists (MLT) at the lab must manually analyse the sample by taking readings with their
naked eyes. This is an inescapable issue because everyone’s capacity to judge and interpret colour
varies. When compared to the findings issued by MLT examined with the naked eye, having a
machine that can execute the analysis automatically can provide more accurate and dependable
results of serum indices. As a result, this study was conducted to compare the findings of manual
MLT analysis with the results of automatic machine analysis on the specimen serum indices. So
that the results of this study can help to solve all of the problems that have previously plagued
laboratories.

KEYWORDS: comparison of serum indices, lipemic, icteric, hemolysis

P H Y L O G E N E T I C A N A LY S I S O F
DENGUE TYPE 3 (DENV3) IN KLANG
VA L L E Y

Mulya Mustika Sari1,2, Sharifah Izwan Tuan Othman1, Tan Kim

Kee2

1 Faculty of Health Sciences, Universiti Selangor
2 Tropical Infectious Diseases Research & Education Centre,
Universiti Malaya

ABSTRACT

Dengue is an acute viral disease transmitted by the infected Aedes mosquito, prevalent in most
tropical urban areas of the world. There are four antigenically distinct dengue virus (DENV)
serotypes, namely DENV type 1 (DENV1), DENV type 2 (DENV2), DENV type 3 (DENV3), and
DENV type 1 (DENV4). The present study was aimed to determine the DENV3 genotype
collected in UniversitiMalaya Medical Centre (UMMC) from 2016 to 2019. Complete genome
sequencing of four and six DENV3 isolates collected in 2016 and 2018, respectively, were
determined. A phylogenetic tree was constructed using the Neighbor-Joining method to
elucidate the relationship of DENV3 collected from UMMC with those previously reported. The
phylogenetic tree analysis showed that four isolates in 2016 were grouped within DENV3
genotype I (DENV3/I), and six DENV3 isolates in 2018 clustered into DENV3 genotype III
(DENV3/III). The epidemiological data showed that clade replacement of DENV3 (DENV3/I to
DENV3/III) did not result in upsurge dengue cases. Phylogenetic analysis of DENV3 collected
between 2016 to 2019 provides a better understanding of virus emergence patterns in the local
setting and their effects on dengue cyclical
patterns in Klang Valley.

KEYWORDS: Dengue, phylogenetic, genotype

P E R F O M A N C E E VA L U AT I O N O F
A U TO M AT E D I MPED AN C EPLAT EL ET
COUNTS IN MICROCYTIC ANAEMIA
AT K K P U L A U PA N G K O R

Nor Azi Kamarudin1,2, Nur Khairul Bariyah Baharun1

1 Faculty of Health Sciences, Universiti Selangor
2 Klinik Kesihatan Pulau Pangkor

ABSTRACT

The accurate assessment of platelet count is a critical component in the diagnosis and treatment of
hemostasis disorder. The aim of this study is to evaluate the perfomance of automated impedance
platelet count in red cell microcytosis sample and to evaluate the impact of microcytosis on
platelet count accuracy using platelet impedance method. In this study, 105 samples with mean
corpuscular volume less than 80 fl were examined. The samples were analysed with a Nihon
Kohden MEK-6510, and each sample was manually counted with a hemocytometer. A total
number of 105 blood samples included in this study have the MCV values of less than 80 fl.
There are 20 blood samples (19.0%%) with MCV of 50-59 fl; 38 blood samples (36.2%) with
MCV of 60-69 fl; and 47 blood samples (44.8%) with MCV of 70-79 fl respectively. 25 patients
collected from data were from male patients (23.8%) and 80 patients were collected from female
patients (76.2%). From the data collected, 65 patients were from Malay patients (52%), 25 were
Chinese patients (23.8%), 15 were Indian patients (14.3%). The differences in mean platelet
counts between PLT-I and PLT-M counting methods implicate that spurious platelet count may
have been released by the PLT-I methods in microcytosis samples. PLT-I This implies a significant
influence in the accuracy of platelet counts in microcytosis blood samples. The correlation
between PLT-M and PLT-I methods was calculated at r = 0.914 (P &lt; 0.05). There was a strong
positive correlation between PLT-I and PLT-M which was statiscally significant (r=.0914, n= 105,
p&lt;0.005). This finding indicates that the platelet count in impedance method is overestimated
as compared to the platelet manual method in microcytosis samples.

KEYWORDS: platelet, impedance platelet count, hemocytometer, microcytic anemia

CHANGES OF ERYTHROCYTE
S E D I M E N TAT I O N RATE, R E D C E L L
DISTRIBUTION WIDTH AND
LY M P H O C Y T E C O U N T IN
PULMONARY TUBERCULOSIS
PAT I E N T S

Nur Farahiyah Che Mohd Nor1,2, Sharifah Izwan Tuan Othman¹

¹Faculty of Health Sciences, Universiti Selangor
²Laboratory Unit, Klinik Kesihatan Lenggong

ABSTRACT

Several research have been carried out to determine the link between pulmonary tuberculosis
(PTB) infection and changes in haematology parameters such as red cell distribution width (RDW)
and lymphocyte count, which leads to anaemic inducing factor. PTB infections cause alterations
in the erythrocyte sedimentation rate (ESR), which corresponds to the inflammation that PTB
patients experience. Variety of haematological abnormalities occurred in association with PTB
but may be under diagnosed or even misdiagnosed causing patients unable to receive a proper
and comprehensive treatments. This study included 50 patients diagnosed with PTB (33 males
and 17 females) who were seen in the outpatient department of Klinik Kesihatan Lenggong.
Sputum smears for acid-fast bacilli, FBC, and ESR measures were performed on all patients. All
smears were stained with Auramine O stain according to usual procedure, and blood was drawn
from the patient and placed in EDTA and ESR containers before being evaluated on an
automated cell counter (Sysmex XP-100 haematology analyser) while ESR was done manually
through Westergren method. Statistical analysis was performed with SPSS version 26.0 (SPSS
Inc, IBM). The chi-square test was used to compare categorical variables. Statistical significance
was defined as a two-tailed P value of less than 0.05. For non-normally distributed data, Spearman
correlation coefficients were used to examine the link between haematological markers. The ESR
and RDW values had a slight but significant connection (p = 0.042) according to Spearmans
correlation analysis. Furthermore, no statistically significant relationships between ESR values
and lymphocyte count (p&gt; 0.05) or lymphocyte count and RDW values (p&gt; 0.05) were
found in PTB patients.

KEYWORDS: Pulmonary tuberculosis, haematological changes

ANTI-BIOFILMS ACTIVITY OF
LACTOBACILLUS AGAINST
S TAPHY LO C O C C US INFECTIOUS: A
REVIEW

Nur Filza Wahi, Norhatiah Md Lias

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

Biofilms is broadly documented by the community of public health as a significant source of
pathogens. One of that pathogenic bacteria formation is Staphylococcus aureus which characterizes
a threat for human’s health causing contagious diseases such as sepsis, abscess formation and
in the worst state can leads to death. Lactobacillus is known as probiotics and prebiotics that
effectively used in the wide range for nutritional, cosmetic products and skin treatments. The
positive feedback interactions between beneficial bacteria-bacteria is also directly involved in
host homeostasis. Thus, the elements of Lactobacillus are recognized to be used as anti-biofilm.
The aim of this study is to determine anti-biofilm effect of Lactobacillus against Staphylococcus
infectious. This study review was done by comprehensive searching manually using Google
Scholar, Science Direct and PubMed. The anti-biofilm activity also based on results of fluorescence
microscope. It showed that Lactobacillus has capability as anti-biofilm against Staphylococcus
infectious strain. This concludes that Lactobacillus invention shows an essential role of organism
potentially to prevent Staphylococcus infectious formation.

KEYWORDS: Keyword: Bio-film, anti-biofilm, Lactobacillus, Staphylococcus infectious

THE AS S O C I ATI O N O F C A R B O N
DIOXIDE LEVELS IN DETECTION OF
METABO LIC ACTIVITY O F B A C T E R I A
IN B L O O D C ULTU R E V I A L S

Nurshafiqah Mohd Sabri , Norhatiah Md Lias

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

Continuous-monitoring automated blood culture system has been found to be used widely in
the Microbiology department. However, after the vials is positive, the time taken for the isolation
of organism from the vials would still takes a long time to be reported. This can cause delayed
process of treatment to the patients. Usually, preliminary report of gram staining will be reported
at the first place, hence, treatment can be started. But this method can cause a slight problem
because gram staining method requires manual analysis where there is possibility of human error
especially during the gram staining procedure and microscopic examination. With the wrong
gram staining result being reported, treatment given to the patients can be mistakenly done. As
we know, septicemia can cause serious complications, as well as fatal. Incorrect treatment can
result in a poor prognosis for the patient. This study is aim to associate the level of carbon
dioxide content with the metabolic activity of bacteria growing in the blood culture vials. It is
done by providing the level content of the carbon dioxide in the positive vials in form of
Relative Fluorescent Unit (RFU) for the BD Bactec FX analyzer and the type of bacteria species
isolated from the positive blood culture vials. This information is useful in the study of bacterial
metabolism and the acknowledgement of a significant content of carbon dioxide to the possible
organisms that is isolated from the positive blood culture vials. It is conclusively proven that there
is a moderate association between the carbon dioxide content and the type of bacteria isolated
from the positive blood culture vials.

KEYWORDS: Blood culture system, bacteremia, gram stain, bacteria metabolism

ESTABLISHMENT OF ESTROGEN RECEPTOR (ER) AND
PROGESTERONE RECEPTOR ( PR ) COLD ISCHEMIC AND
FIXATION TIME OF THE BREAST CARCINOMA AT HOSPITAL
TUANKU JAAFAR, SEREMBAN, NEGERI SEMBILAN

Nurul Ain Ruslan1,2, Asma Harun1, Alizah Ahmad2

1Faculty of Health Sciences, Universiti Selangor
2 Hospital Tuanku Jaafar

ABSTRACT

Proteins known as estrogen receptors and progesterone receptors have been found in breast cancer
tissue cells. Estrogen and related hormones, such as progesterone, are required for cancer cells with
these receptors to proliferate.. Tests must be completed out by an accredited laboratory that complies
to standard testing methods and processes materials consistently.. A standard component of a breast
cancer diagnosis is a test for estrogen and progesterone receptors in the tumor. The accuracy of the
results was important because these are used for the best treatment. Immunohistochemistry, or IHC, is
the most effective method of detecting estrogen and progesterone receptors in a tumor. From a
sample of tissue, IHC testing can reveal estrogen and progesterone receptors in cancer cells. In order to
get the best results, a guideline has been issued by ASCO-CAP to obtain standards in the diagnosis of
these breast cancer cases. Among the factors at the focal point were the pre analytical, which was a
cold ischemic time, and an optimal time for tissue fixation in 10% buffered formalin. This study was
conducted to evaluate the effectiveness of the ischemic time range and tissue preservation time within
10% buffered formalin against the quality and intensity of ER and PR staining results. All of these
articles also attempt to demonstrate whether the ASCO-CAP guidelines affect the quality factors and
results of ER and PR staining. A total of 14 writings from 14 authors were chosen to help with this
study. There are various method conducted for the same purpose. The results showed no significant
differences in results, and the intensity of ER and PR staining on pre analytical factor before the ER and
PR immunohistochemical tests were performed. However, the impact on quality for ER and PR tests
was influenced by the delay in formalin fixation and the prolong time range for tissue preservation in
formalin.

Keywords: fixation, histopathology, IHC, cancer

RHESUS PHENOTYPING AMONG
D O N O R S IN T E L U K INTAN H O S P I TA L

Nurul Izyan Mohamad Anuar1,2, Mohd Jaamia Qaadir Mohd Badrin1

1 Faculty of Health Sciences, Universiti Selangor
2 Hospital Teluk Intan

ABSTRACT

Rhesus phenotypes are the second most prevalent in the blood system and have clinical relevance
for patients. A blood bank’s primary role is to ensure that patients receive safe and compatible
blood and blood components in a timely way. Apart from ABO, the Rhesus (Rh) blood type system
is the major blood group system used in transfusion medicine. The purpose of this study is to
classify Rhesus phenotypes among blood donors using Fisher & Race and Weiner terminologies
and then to determine the most prevalent phenotype. Additionally, this research was undertaken
to see whether there is a substantial correlation between Rhesus phenotypes, ethnicity and
blood group among donors. A total of 100 segmented random blood donor consist of (57 Malay,
32 Chinese, and 11 Indian) were collected. Only rhesus phenotyping were performed on each
sample because the blood bag already printed with representative blood group. The result show
that Malay is the most contributor in blood donation with 57 donors. The most frequent blood
group is O positive with 43% and the probable phenotype is R1R1 with 57%. The relationship
between phenotype and race showed that Malay phenotype is 34 donors. The relationship
between phenotype and blood group showed that O positive is the main blood group. Both
result showed that the statistically not significant between two relationship. The conclusion is
blood groups do play a role in the formation of ethnicity. These blood groups must be linked to
evolution, and it probably shows how close different races are to each other. The need for blood
and blood products is growing, as well as the advancements in transfusion medicine and science,
and the growing trend of getting people from all over the world closer together.

KEYWORDS: rhesus phenotype, blood donor, ABO blood group

PROFILING MUS C ULO S K ELETAL
DISCORMFORT A N D ASSOCI ATE
R I S K FA C TO R S A M O N G WA R E H O U S E
W O R K E R S AT B U K I T BERUNT U N G,
RAWANG, S E L A N G O R

Nurulain Aqilah Dharmawi, Mohd Zubairy Shamsudin , Noormaliza
Hamdan

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

Manual working handlers are particularly vulnerable to risk factors for musculoskeletal disorders.
Malaysia as the one of developing country, must be addressed this issue to ensure do not cause
any damage of the physical and mental of workers. As a result, it is produce safe and health
work environment and can reduce any injuries that relate with manual handling task at work.
Objectives : This study aimed to i) to determine musculoskeletal discomfort among warehouse
workers; ii) to identify the risk factor among warehouse workers; iii) to examine the relationship
between musculoskeletal discomfort and risk factor among warehouse workers. Method : The
data were collected using the self-reported questionnaire, Nordic Musculoskeletal Questionnaire
(NMQ) and ergonomics risk assessment worksheet, Rapid Entire Body Assessment (REBA)
among 38 warehouse workers at Selayang and Rawang, Selangor. Result : The result shows that
the highest prevalence of musculoskeletal disorders within the past 12 months was lower back
(92.1%), followed by shoulders (86.8%) and wrist/hands (78.9%), knee (76.3%), neck (73.3%),
hips/thighs (71.1%), elbow (57.9%), upper back (50.0%) and ankle/feet (26.3%). For REBA scoring
34(89.5%) respondents gained score more than 11, which mean in very high risk score level for
body posture. By using Chi-Square test, it shows that there was a significant relationship between
musculoskeletal discomfort and risk factor among warehouse workers with p-value (&lt;0.05).

KEYWORDS: musculoskeletal discormfort, risk factor, nordic musculoskeletal questionnaire, rapid entire
body assessment.

FULL B L O O D COUNT PA R A M E T E R
AND C-REACTIVE PROTEIN LEVEL
IN COVID-19 PATIENT IN H O S P I TA L
TENGKU AMPUAN AFZAN

Raja Nor Arfanizam Raja Idris1,2, Asma Harun1, Shariza Ilyana Saupi2

1 Faculty of Health Sciences, Universiti Selangor
2 Hospital Tengku Ampuan Afzan

ABSTRACT

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was a causative agent that
responsible for the pneumonia outbreak or COVID-19 in Wuhan during late December 2019.
Various study has indicated that the infection of COVID-19 not only affect respiratory system
but also altered the haematopoietic system as well. Current study investigated the association
between the parameter of full blood count test particularly for haemoglobin, platelet, white blood
cell count and differential types of white blood cells and evaluation of c-reactive protein level in
two categories of positive COVID-19 patients. A total number of 50 patients were selected from
Pusat Kuarantin Rawatan COVID-19 (PKRC) and ICU ward that represented for mild and severe
category respectively. There are three principles applied to evaluate the values of each parameter
in full blood count test from whole blood samples which includes flow cytometry, direct current
sheath flow and Sodium lauryl sulfate (SLS) -Hemoglobin channel, and the result were tabulated
according to the normal ranges of the variables. C-reactive protein were analyzed based on
Immuno-turbidimetric principle for the quantitative determination of C-reactive protein in serum
samples. The statistical analysis founded that there were significant association between the
values of hemoglobin, white cells count and c-reactive protein in two groups of subjects (p value
<0.05). Nonetheless, there was no significant difference in the values of platelet in both groups
(p value >0.05). The result of each type of white blood cells revealed that only neutrophils and
lymphocytes has meaningful difference against groups of research participants (p value <0.05),
that might be considered as the most affected types of white blood cells in COVID-19 infection.
Finally, due to the limited scope of research in this field, the analysis may provide additional
information as well as ideas for future studies.

KEYWORDS: COVID-19, SARS-CoV-2, Full blood count, C-reactive protein, White blood cells, Hemoglobin,
Platelet, Neutrophil, Lymphocyte, Monocyte, Eosinophils, Basophils, Cytokine storm, Thrombopenia,
Leukocytosis, Lymphopenia

DETECTION OF COMMUNITY
A C Q U I R E D METHICILLIN RESIS TANT
S TAPHY LO C O C C US A U R E U S IN
C LI N I C A L S A M P L E HO SPITAL
P U T R A J AYA U S I N G D I S C D I F F U S I O N
METHOD

Rozihatullaily Ahmad Radzi1,2 , Sarimah Mat Nawi1

1Faculty of Health Sciences, Universiti Selangor
2 Hospital Putrajaya

ABSTRACT

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that causes infections in
different parts of the body. It is tougher to treat than to prevent from most strains of S. aureus
because of its resistant to some commonly used antibiotics. Arguments on misdiagnosis has been
a controversial issue. Molecular typing methods have been produced to define the transmission
routes and the source of infection during epidemiological investigations in order to ward off
these events. The purpose of this study is to assess methicillin-resistant by phenotypic approach
to compare with disc diffusion method and Polymerase Chain Reaction (PCR) outcome. A total
of 1392 samples were obtained from clinical samples at Hospital Putrajaya. 54% of the samples
are from females, whereas 46% come from males. Nine (9) isolates were categorised as true
CA-MRSA based on the CDC criterion of no hospital-associated risk factors and the results of
SCCmec typing. Among them were eight (8) isolates positive for SCCmec type IV.Given the
rising incidence of MRSA infections, the development of reliable, accurate, and speedy assays
for MRSA detection is critical for both antibiotic therapy and infection control measures.This
emphasises the need of diagnosing CA-MRSA using established diagnostic procedures such as
molecular characterisation using polymerase chain reaction (PCR) and antibiotic susceptibility
profiles in order to avoid treatment mistakes.Effective control of MRSA transmission in the
community will very certainly necessitate effective control of nosocomial MRSA transmission.
Handwashing, using gloves, gowns, and masks, isolating MRSA patients, often decolonizing the
hospital environment, and using suitable disinfectants in clinical settings are all likely to help
prevent MRSA spread.

KEYWORDS: methicillin, pcr, MRSA, Staphylococcus aureus

C H ARAC TERI Z ATI O N O F H A REGIO N
O F I N F L U E N Z A B V I R U S I S O LATED
F R O M S E V E R E AC U TE RES P I RATO RY
I N F E C T I O N ( S A R I ) PATIENTS

Sheila Koh Siau Yee1,2, Norhatiah Md Lias1, Tengku Rogayah Tengku Abd
Rashid2

1 Faculty of Health Sciences, Universiti Selangor
2 Virology Unit, Institute for Medical Research

ABSTRACT

Studies found influenza B virus can cause severe illness and mortality in some hospitalized cases.
Therefore, there is the potential involvement of mutation in the HA region of Influenza B virus
isolated from severe acute respiratory infection (SARI) patients’ samples associated with varying
severity or mortality rates caused by Influenza B. This study aims to characterize the hemagglutinin
(HA) region of the influenza B virus isolated from SARI patients’ samples. There were random 62
archived SARI respiratory samples recruited from Virology Unit, Institute for Medical Research
(IMR). 18 out of 62 (29%) SARI samples have shown Ct value ≤30 and selected RNA samples
were proceeded to amplification of HA region. Partial of HA genes in these selected samples
were sequenced using reverse transcription- polymerase chain reaction (RT-PCR) and analyzed
using bioinformatics software (ChromasPro and MEGA 6.0). Based on the phylogenetic analysis,
all the isolates were belonged to B/Victoria lineage, and there were 3 differences in nucleotide
(location 395, 471, and 585) of our isolated partial HA gene compared to B/Idaho/02/2020 strain.
However, it concluded as a silent mutation. Moreover, there were no nucleotides differences
between our isolates and non-severe SARI cases on GenBank. However, few nucleotide changes
were found when our isolates compared to severe SARI cases on GenBank and it also appeared
as silent mutations. Therefore, the variety of severity and mortality among SARI patients believed
not associated with the mutation in the HA region. Fresh samples with a higher yield of RNA
concentration are highly recommended to be included in inclusion criteria and the recommended
sample size by Raosoft® should be achieved for future studies.

KEYWORDS: Influenza B Virus, Hemaglutinin (HA), Charaterization, Mutation, Severe Acute Respiratory
Infection (SARI)

E VA L U AT I O N O F L E U C O C Y T E S
DIFFERENTIAL COUNT USING 3
PA RT S A N D 5 PA RT S H A E M ATO L O G Y
A N A LY S E R A M O N G C A N C E R
PATIENTS IN K L I N I K K E S I H ATA N
DARO

Siti Hanisah Abu Bakar1,2, Yusmi Mohd Yunus1

1 Faculty of Health Sciences, Universiti Selangor
2 Klinik Kesihatan Daro

ABSTRACT

Chemotherapy is a treatment for cancer patient. This treatment is used to kill the damage or kill
cancer cells. Various medicines used as chemotherapy to treat cancer can cause temporary drops
in WBC numbers. White blood cell numbers begin to decline a few days after chemotherapy is
delivered and reach their lowest point in the second or third week. Blood counts are checked
before each chemotherapy round to confirm that they have returned to the normal range. Blood
tests to assess cell counts are performed at regular intervals throughout chemotherapy. Complete
Blood Counts (CBC), or ‘Hemograms,’ are the terms for these tests. Total leukocyte counts, or
TLCs, are a measure of the number of white blood cells in the body. The absolute neutrophils
count is a more precise count (ANC). Neutrophils are a kind of white blood cell, and their numbers
are a good predictor of how effectively the body can handle bacterial infections. Therefore, this
study was done to evaluate the Leucocytes Differential Count (LDC) using 3-parts analyser and
5-parts analyser among cancer patient. The three-part analyzer can distinguish between three
distinct types of WBCs: neutrophils, lymphocytes, and monocytes. Basophils and eosinophils
cannot be distinguished. Meanwhile, all WBC kinds may be distinguished using a 5-part cell
counter (neutrophils, lymphocytes, basophils, eosinophils, and monocytes). In this study, only
three types of white blood cells will be evaluated (Neutrophile, Lymphocyte and Monocytes). The
statistical analysis indicates that there is no significant difference in the Neutrophile, Lymphocytes
and Monocyte cells count in 3-parts and 5-parts analyser which is the p-value > 0.05. Therefore,
it was concluded that the three white blood cells count are same but the 3-parts analyser cannot
used as the analyser to process the samples for cancer patients because it cannot count the other
two white blood cells, eosinophile and basophile.

KEYWORDS: haematology analyzer, leukocytes differential counts, cancer

GENE EXPRESSION PROFILING IN
TH E L I V E R O F G O TO - K A K I Z A K I RAT
TREAT E D W I T H S A G O RES I S TA N T
S TARC H

Siti Mastura Abdul Aziz1,2, Nazif Samat@Darawi1

1 Faculty of Health Sciences, Universiti Selangor
2 Endocrine Unit, Institute for Medical Research

ABSTRACT

Resistant starch (RS) administration has been known able to improve the glucose intolerance
and insulin resistance. In particular, RS Sago (Metroxylon sagu) was reported to be associated
with postprandial hyperglycemia improvement and diabetes management such as carbohydrate
digestion inhibition in the gastrointestinal tract. However, the antidiabetic properties of RS Sago
from Sarawak associated with hepatic glucose production has not been reported elsewhere and
the mechanisms are still uncertain especially at the molecular level. Furthermore, an understanding
of these gene expression profiles is valuable for potential effective and affordable health
supplements. Thus, the objective of this study is to identify the genes associated with glucose
lowering effects by RS Sago using samples from diabetic rats Goto-Kakizaki liver tissues that
have been administered with water (control), Hi-Maize (positive control), sago RS2 and RS4 using
Rat Glucose Metabolism RT² Profiler PCR Array which consist of 84 genes. The results showed
several significantly up and downregulated genes in Sago treated diabetic rats. RS2 displayed a
significantly upregulated Taldo1 gene whereas G6pc, Sdhb and Rplp1 genes were significantly
downregulated. As for RS4 treated diabetic rats, Idh3g gene was significantly upregulated
whereas G6pc, Pdk3, Eno3, Sdhb, Galm and Tkt genes were significantly downregulated. These
genes involve in the glucose and glycogen metabolisms which associated with blood glucose
homeostasis. In conclusion, our local sago is highly potential in managing diabetes as the genes
identified might be useful as therapeutic targets in reducing hepatic glucose output

KEYWORDS: Type 2 diabetes; Goto-Kakizaki; sago resistant starch; gene expression array; hepatic glucose
regulation

THE RELATI O N S H I P B E T W E E N LUNG
F U N C T I O N STATUS A N D S Y M P TO M S ,
A C T I V I T Y A N D S O C I A L L I F E I M PA C T S
OF WELDERS IN BANGI, SELANGOR

Siti Nur Syahirah Jasni, Fauzana Mustaffa, Erna Haryani Amaludin

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

One of the common process in the industries such as construction, mining, petrochemical and
shipping is welding. A small quantity of metals is vaporized by heat and welding fumes are emitted
into the air, which can affect the health of the welders. Inhalation exposure were involved in
welding process and they resulted in chronic respiratory disease. In Malaysia, chemicals
identified as hazardous to health with their allowable emission limits (PEL) have been listed
under the Occupational Safety and Health Act 1994, Use and Standards of Exposure of Chemical
Hazardous to Health Regulation (USECHH) and must be complied with by the employer. This
study is conducted to determine the correlation between lung function status and symptoms,
activity and social-life impacts on welders. This study has taken place in grille fabrication shops
in Bangi, Selangor. A non-probability sampling method were used for 35 respondents in this
study which involves method of measuring lung function status and distribution of St George
Respiratory Questionnaires. Based on the data, the result had showed that there is were significant
correlation between lung function and symptoms p = 0.010, activity, p = 0.186 and social-life
impacts, p = 0.60. In order to reduce the exposure of the symptoms in breathing difficulties it has
to eliminate fumes from the area and guarantee that there is enough clean breathing air which is
use an exhaust hood. In conclusion, there is significant relationship between lung function and
symptoms, activity and social-life impacts on welders.

KEYWORDS: lung function status, symptoms, activity, social-life impacts, welders, welding, Spirometer
(Hi-101 chestgraph), St George Respiratory Questionnaire (SGRQ)

CHEMOTHERAPHY EFFECTS
O N TUMOR M A R K E R CA19.9,
HEMOGLOBIN, WHITE CELL COUNT
A N D P LATEL ET

Vevianpreet Kaur Hullon Baldev Singh, Mohd Jaamia Qaadir Mohd
Badrin

Faculty of Health Sciences, Universiti Selangor

ABSTRACT

Chemotherapies are well-known for their impact on haematological profiles, including the gradual
depletion of haematopoietic stem cells in bone marrow. Despite evidence that chemotherapy
has significant side effects, particularly on the haematological profile, there has been little study
on the changes in haematological parameters in pancreatic cancer patients before and after
chemotherapy. The intent of this study is to evaluate the effects of chemotherapy on the tumour
marker CA19-9, haemoglobin, white cell count, and platelet count. Serum CA 19-9 levels have
been investigated in a number of clinical situations to see whether they may be utilised as a
surrogate measure of chemotherapy response. There is a substantial variation in the ca19.9,
haemoglobin, white cell count, and platelet findings of patients with Ca19.9 of all ages and sexes
following chemotherapy sessions that are lower, normal, or greater white cell count and platelets
from the prior count. All cancer patients with detailed demographic information such as age,
gender, diagnosis, and treatment, as well as medical data such as haematological profiles in pre-
and post-chemotherapy treatments. Cancer is also significantly associated to age, as per the study
findings. Chemotherapies can have an effect on a person’s Red Blood Cells, and with a lower
RBC count, the amount of oxygen moving through the body decreases. Following chemotherapy,
bone marrow cells are unable to make RBCs at a regular pace. In terms of white blood cell level,
the results show that the difference in mean score between post and pre chemotherapy patients
is not statistically significant. In terms of platelet level, the results show that the difference in
mean platelet levels between post and pre chemotherapy patients is not statistically significant.
Despite its flaws, chemotherapy remains a significant therapeutic approach in oncology and will
likely stay so for a long time. As a consequence, the major purpose of the study, which was to
determine the levels of CA19-9, white blood cell, and platelet count in pancreatic cancer patients
before and after therapy, was met.

KEYWORDS: Chemotherapy, cancer patients, white cell count, platelet, haemoglobin, CA19.9

CORE COMMITTEE LIST

PATRON:

Mohd Sharikh Dali bin Mahad
(Dean, Faculty of Health Sciences)

Madam Izreen bt Supaat
(Deputy Dean – Academic)

Ts. Sawal bin Marsait
(Deputy Dean, Student Development and Resources)

Ts. Mohd Jaamia Qaadir bin Mohd Badrin
(Head of Program, Bachelor in Medical Laboratory Science (Hons)

Madam Noormaliza bt Hamdan
(Head of Program, Bachelor of Occupational Safety and Health (Hons)

Advisor : Dr. Yusmi bin Mohd Yunus
(Lecturer - Scientific Communication MLS 3243)
Chairperson
Co-Chairperson : Muhammad Nadzwan Aizad bin Ismail
Secretary : Vevianpreet Kaur Hullon A/P Baldev Singh
Co-Secretary : Aqilah Nabilah binti Suliman
Treasurer : Nurul Ashikin binti Mohamed Shahrehan
: Nurulain Aqilah binti Dharmawi

WORKING COMMITTEE LIST

Chairperson : Muhammad Nadzwan Aizad bin Ismail
Co-Chairperson : Vevianpreet Kaur Hullon A/P Baldev Singh
: Aqilah Nabilah binti Suliman
Secretary : Nurul Ashikin binti Mohamed Shahrehan
: Nurulain Aqilah binti Dharmawi
Co-Secretary

Treasurer

SPECIAL BUREAU : Muhammad Shahir bin Mohd Redzuan
Registration Bureau : Ismadi bin Surry Lisius

Publicity Bureau : Rozihatullaily binti Ahmad Radzi
: Nurshafiqah binti Mohd Sabri
: Thipanithran A/L Subramaniam
: Kishaanthan A/L Balkrishnan
: Asvini A/P Manikian

Blogspot Bureau : Edmond Ubil bin Zulkifli
: Nurul Izyan bte Mohamad Anuar
: Muhammad Nur Hidayat bin Mohd Janny
: Mohd Faiz Shazwan bin Mohd Pauzi
: Indriani binti Indar
: Azreen Zariza binti Alias

Facebook Page : Shiqah binti Mohd Razali
Bureau : Rosyida Nabila binti Rozali
: Nor Azi binti Kamarudin
Protocol Bureau : Nur Farahiyah binti Che Mohd Nor
: Raja Nor Arfanizam binti Raja Idris
Certificate Bureau : Eric Sharvin A/L David

: Muhammad Ammar bin Abdul Rahman
: Nur Feriza binti Mohamad Fozi
: Siti Nur Syahirah binti Jasni
: Jayaram A/L Sakaraj
: Christine Ricky
: Siti Mastura binti Abdul Aziz

: Sheila Koh Siau Yee
: Angelyn Ann Yeo