Annual Report 2010-11

Table 23. Status of initial and post harvest soil in the experiment.

Analytical value

Treatments pH OC (%) Total N Avail. P Exch. K (meq%) Avail. S
(%) (ppm) (ppm)
Initial soil 0.16
Post harvest 7.6 0.66 0.07 11.0 19.0
V1K0 0.12
V1K45 7.60 0.67 0.060 8.0 0.14 19.0
V1K90 7.65 0.57 0.066 9.0 0.16 20.0
V1K135 7.60 0.71 0.050 13.0 0.18 20.5
V2K0 7.61 0.71 0.060 12.0 0.13 19.0
V2K45 7.65 0.53 0.060 9.0 0.14 20.0
V2K90 7.77 0.50 0.077 10.0 0.16 20.5
V2K135 7.68 0.71 0.066 11.0 0.16 20.5
V3K0 7.21 0.46 0.060 11.0 0.13 20.0
V3K45 7.60 0.67 0.056 6.0 0.15 19.0
V3K90 7.62 0.64 0.060 10.0 0.16 20.5
V3K135 7.50 0.51 0.060 11.0 0.18 17.5
V4K0 7.58 0.71 0.066 11.0 0.12 19.0
V4K45 7.59 0.64 0.050 9.0 0.14 18.0
V4K90 7.62 0.67 0.060 9.0 0.15 20.5
V4K135 7.65 0.67 0.066 12.0 0.18 20.5
V5K0 7.67 0.53 0.060 10.0 0.13 20.0
V5K45 7.60 0.71 0.055 8.0 0.13 19.0
V5K90 7.65 0.67 0.060 11.0 0.15 20.0
V5K135 7.61 0.78 0.060 11.0 0.16 20.5
7.65 0.57 0.060 11.0 19.0

Thakurgaon site (AEZ - 1) of 61.00 t ha-1 was observed in clone I 112-01 where
Yield and yield attributes 180 kg N ha-1 was applied followed by V2N180 (60.50
In N experiment there was a significant (p<0.05) t ha-1), V2N120 (60.33 t ha-1), V3N120 (54.33 t ha-1),
interactive effect of clones/variety and rates of V3N0 (53.17 t ha-1), V2N0 (51.83 t ha-1) and V2N60
fertilizer application on millable cane, height, brix and (51.50 t ha-1) respectively. The lowest yield of 36.17 t
yield production. Clones/variety and rates of fertilizer ha-1 was found in variety Isd 38 where 0 kg N ha-1
application on yield ranged from 36.17 to 61.00 t ha-1 was applied.
in all the treatments (Table 24). Maximum cane yield

Table 24. Effect of Nitrogen on the yield and yield attributes of sugarcane at RSRS farm sugarcane at
RSRS farm (2009-2010).

Treatment Tiller Millable cane Girth Height Yield Brix
combinations (× 103 ha-1) (× 103 ha-1) (cm) (m) (tha-1) %

V1N0 88.67 ab 76.7bcd 1.993 d 2.59 b 36.17 g 20.84 a
V1N60 83.33ab 85.0 abcd 2.190 abcd 2.59 b 48.00 cdef 19.33 abcd
V1N120 87.00ab 2.070 abcd 2.63 b 18.60 bcd
V1N180 85.33ab 95.0 a 2.160 abcd 2.60 b 39.00 fg 19.63 abcd
V2N0 81.00 b 84.2abcd 2.330 abc 2.72 ab 38.67 fg
V2N60 83.33ab 85 abcd 2.160 abcd 2.82 ab 51.83abcde 20.27 ab
V2N120 85.33ab 82.50 abcd 2.88 ab 51.50abcde 19.97 abc
V2N180 90.33ab 80.00 bcd 2.337 ab 3.02 a 60.33 ab 19.97 abc
V3N0 97.67ab 76.67 bcd 2.373 a 2.70 ab 60.50 ab 19.87 abcd
V3N60 91.33ab 86.67 abc 2.073 abcd 2.52 b 53.17 abcd 19.33 abcd
V3N120 85.33ab 87.50 abc 2.300 abcd 2.79ab 49.00 cdef 19.70 abcd
V3N180 92.00ab 73.83 cd 2.270 abcd 2.79ab 54.33 abc 19.00 abcd
V4N0 92.00ab 81.67 abcd 2.103 abcd 2.83ab 61.00 a 18.00 d
V4N60 99.67a 81.67 abcd 2.027 bcd 3.03 a 42.83 defg 18.67 bcd
V4N120 91.00ab 83.33 abcd 2.140 abcd 2.81ab 49.67bcdef 18.93 abcd
V4N180 93.67ab 78.33 bcd 2.150 abcd 2.64 b 42.17 defg 18.27 cd
87.50 abc 2.000 cd 49.00 cdef 18.33 bcd

Soils & Nutrition Division 43

Treatment Tiller Millable cane Girth Height Yield Brix
combinations (× 103 ha-1) (× 103 ha-1) (cm) (m) (tha-1) %

V5N0 97.67ab 72.50 d 2.110 abcd 2.78ab 39.50 fg 19.53 abcd
V5N60 85.33ab 88.50 ab 2.180 abcd 2.87ab 44.50cdefg 19.03 abcd
V5N120 89.33 ab 85.00 abcd 2.027 bcd 2.83ab 40.50 efg 19.33 abcd
V5N180 101.0 a 76.50 bcd 2.063 abcd 2.88ab 40.00 fg 19.17 abcd
LSD ( 0.05) 0.3136
15.10 11.40 0.2766 9.719 1.614

In a column, the figures having same letter do not differ significantly as per DMRT at 5% level.

Soil nutrient status after sugarcane harvest
There are slight decreasing tendency was observed in case of S over initial soil, other nutrients remained
unchanged (Table 25).

Table 25. Status of initial and post harvest soil in the experiment.

Analytical value

Treatments pH OC (%) Total N Avail. P Exch. K (meq%) Avail. S
(ppm)
Initial soil 5.10 1.51 (%) (ppm) 0.17
Post harvest 25.00
V1N0 5.06 1.50 0.09 22.00 0.16
V1N75 5.00 1.45 0.15 22.0
V1N150 5.10 1.44 0.08 20.0 0.15 21.5
V1N225 5.08 1.40 0.08 21.0 0.16 22.5
V2N0 5.05 1.51 0.09 19.5 0.15 22.5
V2N75 5.02 1.48 0.10 20.0 0.16 21.0
V2N150 5.08 1.46 0.07 20.0 0.17 21.0
V2N225 5.10 1.42 0.08 19.0 0.17 21.5
V3N0 4.98 1.45 0.08 20.0 0.16 22.0
V3N75 5.00 1.50 0.08 20.0 0.16 21.0
V3N150 5.00 1.35 0.075 19.0 0.17 22.0
V3N225 5.06 1.38 0.075 20.5 0.16 22.5
V4N0 5.00 1.38 0.08 21.0 0.15 23.0
V4N75 5.01 1.40 0.10 21.0 0.16 21.5
V4N150 5.06 1.45 0.07 20.0 0.16 22.0
V4N225 5.08 1.48 0.075 21.0 0.17 23.0
V5N0 5.06 1.40 0.08 21.0 0.16 22.0
V5N75 5.10 1.45 0.09 20.0 0.17 22.0
V5N150 5.06 1.48 0.075 19.0 0.17 21.0
V5N225 5.08 1.48 0.08 19.5 0.16 21.5
0.08 19.0 22.5
0.095 19.0

In P experiment, there was a significant (p<0.05) cane yield of 80.50 tha-1 was observed in Clones
interactive effect of clones/varieties and rates of I 95-01 where 60 kg P ha-1 was applied followed by
fertilizer application on millable cane, height, girth, V2P40 (70.33 t ha-1) and V3P60 (67.0 t ha-1)
brix and yield production. Clones/varieties and rates respectively. The lowest yield of 38.50 tha-1 was
of fertilizer application on yield ranged from 38.50 to found in variety Isd 38 where 40 kg P ha-1 was
80.50 t ha-1 in all the treatments (Table 26). Maximum applied.

44 Annual Report, 2010-2011

Table 26. Effect of Phosphorus on the yield and yield attributes of sugarcane at RSRS farm.

Treatments Tiller Millable cane Girth Height Yield Brix
(× 103 ha-1) (× 103 ha-1) (cm) (m) (tha-1) %
18.15 ab
V1P0 92.00 bc 89.17 a 1.95 bcd 2.48 e 39.50 gh 18.67 ab
V1P20 106.3 abc 93.83 a 1.84 d 2.62 cde 44.67 fgh 18.57 ab
V1P40 112.0 abc 81.33 a 1.92 bcd 2.87 abcd 38.50 h 18.20 ab
V1P60 98.33 abc 90.50 a 1.96 abcd 2.72 bcde 45.83 efgh 18.13 ab
V2P0 104.7 abc 93.17 a 2.27 a 18.89 a
V2P20 90.33 c 91.67 a 2.13 abcd 2.96 ab 66.00 bc 18.60 ab
V2P40 100.0 abc 91.67 a 2.11 abcd 2.99 ab 63.67 bcd 18.73 a
V2P60 98.00 abc 101.7 a 2.20 abc 2.75 abcde 70.33 ab 18.13 ab
V3P0 99.33 abc 93.50 a 2.12 abcd 2.90 abc 18.33 ab
V3P20 107.7 abc 93.83 a 2.22 ab 2.81 abcde 80.50 a 18.47 ab
V3P40 98.67 abc 103.7 a 2.08 abcd 2.76 abcde 54.17 cdefg 17.63 b
V3P60 97.00 abc 92.17 a 2.27 a 2.86 abcd 56.50 bcdef 18.80 a
V4P0 94.17 a 2.10 abcd 2.77 abcde 58.83 bcdef 18.33 ab
V4P20 117.7 a 89.33 a 1.96 abcd 3.05 ab 18.23 ab
V4P40 111.3 abc 93.83 a 1.90 cd 3.08 a 67.00 abc 17.83 ab
V4P60 106.0 abc 91.67 a 2.02 abcd 2.99 ab 59.67 bcdef 17.90 ab
V5P0 106.0 abc 87.17 a 1.99 abcd 3.06 ab 59.67 bcdef 18.07 ab
V5P20 98.67 abc 91.67 a 2.06 abcd 2.75 abcde 56.33 bcdef 18.83 a
V5P40 98.83 a 2.02 abcd 2.54 de 61.00 bcde 18.33 ab
V5P60 116.7 a 95.50 a 2.13 abcd 2.83 abcd 50.33 defgh 0.8824
LSD ( 0.05) 114.3 ab 2.75 abcde 45.50 efgh
116.0 a 0.267 58.17 bcdef Avail. S
0.286 47.83 efgh (ppm)
19.04 22.0
13.26
20.0
In a column, the figures having same letter do not differ significantly as per DMRT at 5% level. 21.0
20.0
Soil nutrient status after sugarcane harvest 22.0
The nutrient status after sugarcane harvest remained unchanged (Table 27). 21.0
21.0
Table 27. Status of initial and post harvest soil in the experiment. 22.0
23.0
Analytical value 22.0
22.0
Treatments pH OC (%) Total N Avail. P Exch. K (meq%) 21.0
23.0
Initial soil 5.05 1.50 (%) (ppm) 0.16 22.0
Post harvest 23.0
V1P0 4.98 1.40 0.09 25.0 0.14 23.0
V1P25 5.00 1.45 0.14 22.0
V1P50 5.00 1.45 0.08 23.0 0.15 21.0
V1P75 5.02 1.48 0.08 26.0 0.15 21.0
V2P0 5.00 1.45 0.075 25.0 0.13 22.0
V2P25 5.00 1.45 0.09 25.0 0.14 20.0
V2P50 5.01 1.48 0.085 23.0 0.14
V2P75 5.05 1.50 0.08 24.0 0.15
V3P0 5.01 1.48 0.08 26.0 0.15
V3P25 5.04 1.50 0.09 24.0 0.14
V3P50 5.01 1.40 0.075 22.0 0.15
V3P75 5.03 1.43 0.08 24.0 0.15
V4P0 5.04 1.35 0.08 24.0 0.13
V4P25 5.03 1.42 0.08 25.0 0.14
V4P50 5.08 1.40 0.08 20.0 0.14
V4P75 5.05 1.42 0.09 23.0 0.14
V5P0 5.02 1.40 0.08 25.5 0.14
V5P25 5.05 1.40 0.085 24.0 0.14
V5P50 5.05 1.43 0.08 22.0 0.14
V5P75 5.06 1.45 0.085 25.0 0.15
0.08 26.0
0.09 26.0

In K experiment, there was a significant (p<0.05) cane yield of 69.83 t ha-1 was observed in clones
interactive effect of clones/varieties and rates of I 95-01 where 65 kg K ha-1 was applied which was
fertilizer application on millable cane, height, girth, statistically significant with other treatments except
brix and yield production. Clones/varieties and rates V2K195 treatment. The lowest yield of 29.50 t ha-1

Soils & Nutrition Division 45

of fertilizer application on yield ranged from 29.50 to was found in clone I 137-03 where 195 kg K ha-1 was
69.83 t ha-1 in all the treatments (Table 28). Maximum applied.

Table 28. Effect of Potassium on the yield and yield attributes of sugarcane at RSRS farm.

Treatments Tiller Millable cane Girth Height Yield Brix
(× 103 ha-1) (× 103 ha-1) (cm) (m) (tha-1) %

V1K0 86.33 a 72.17 a 1.887 a 2.44 fg 32.17 ef 18.27 a
V1K65 103.0 a 85.83 a 1.863 a 2.63 defg 39.50 cdef 18.93 a
V1K130 91.67 a 82.33 a 2.040 a 2.46 fg 35.83 cdef 19.17 a
96.33 a 81.50 a 1.970 a 2.66 cdefg 34.17 def 19.27 a
V1K195 89.33 a 72.50 a 2.107 a 3.03 abcde 18.67 a
V2K0 94.67 a 79.67 a 2.080 a 2.94abcdef 51.67 bc 18.60 a
87.00 a 81.67 a 1.970 a 3.14 abcd 69.83 a 18.23 a
V2K65 93.33 a 89.17 a 2.177 a 3.22 ab 46.33 cdef 19.07 a
V2K130 88.67 a 71.83 a 1.917 a 2.70 cdefg 65.17 ab 18.73 a
87.00 a 71.83 a 1.910 a 38.17 cdef 18.73 a
V2K195 96.00 a 83.33 a 2.053 a 2.40 g 48.00 cde 18.60 a
V3K0 90.33 a 86.33 a 2.137 a 2.66 cdefg 38.17 cdef 18.83 a
V3K65 96.67 a 78.17 a 1.960 a 2.51 efg 36.83 cdef 18.60 a
95.33 a 81.33 a 1.850 a 2.62 defg 35.50 cdef 18.37 a
V3K130 87.00 a 90.17 a 2.093 a 46.50 cdef 18.87 a
V3K195 86.00 a 91.17 a 1.980 a 3.35 a 43.33 cdef 18.07 a
V4K0 110.3 a 70.00 a 1.907 a 2.73bcdefg 51.00 bcd 18.67 a
V4K65 97.00 a 78.33 a 1.993 a 2.93abcdef 32.83 ef 18.67 a
103.0 a 82.67 a 1.897 a 2.71 cdefg 35.17 cdef 19.23 a
V4K130 103.7 a 84.17 a 2.193 a 2.68 cdefg 32.83 ef 18.23 a
V4K195 0.3621 3.16 abc 29.50 f
2.60 efg
V5K0 14.40
V5K65 0.4373

V5K130
V5K195

LSD ( 0.05)

In a column, the figures having same letter do not differ significantly as per DMRT at 5% level.

Soil nutrient status after sugarcane harvest

The nutrient status after sugarcane harvest remained unchanged (Table 29).
Table 29. Status of initial and post harvest soil in the experiment.

Analytical value

Treatments pH OC (%) Total N Avail. P Exch. K (meq%) Avail. S
(%) (ppm) (ppm)
Initial soil
Post harvest 5.05 1.51 0.08 23.0 0.17 22.0
V1K0
V1K45 4.98 1.45 0.07 22.0 0.15 19.0
V1K90 5.00 1.48 0.07 21.0 0.15 20.0
V1K135 5.01 1.48 0.08 23.0 0.16 20.0
V2K0 5.05 1.49 0.08 22.0 0.16 21.0
V2K45 5.03 1.46 0.07 21.0 0.14 20.0
V2K90 5.05 1.49 0.08 22.0 0.15 21.0
V2K135 5.10 1.50 0.08 23.0 0.15 20.0
V3K0 5.02 1.50 0.07 25.0 0.17 21.0
V3K45 4.98 1.45 0.07 23.0 0.15 21.0
V3K90 5.00 1.48 0.07 24.0 0.15 21.0
V3K135 5.26 1.51 0.09 25.0 0.16 22.0
V4K0 5.03 1.50 0.09 24.0 0.16 23.0
V4K45 5.00 1.48 0.08 22.0 0.14 19.0
V4K90 5.05 1.48 0.09 23.0 0.15 20.0
V4K135 5.00 1.50 0.09 23.0 0.17 20.0
V5K0 5.01 1.48 0.08 24.0 0.16 22.0
V5K45 5.04 1.45 0.075 23.0 0.15 20.0
V5K90 5.05 1.49 0.09 25.0 0..16 22.0
V5K135 5.05 1.50 0.08 23.0 0.17 22.0
5.07 1.50 0.09 23.0 0.17 21.0

46 Annual Report, 2010-2011

SITE SPECIFIC FERTILIZER REQUIREMENT FOR (Fertilizer as per STV for HYG), T4- Sugarcane
SUSTAINABLE PRODUCTION OF SUGARCANE [Fertilizer as per FRG’05] Intercrop (Garlic), T5-
AND INTERCROP IN NON-MILLS ZONE Sugarcane [Fertilizer as per STV for MYG*]
Generating database on the nutrient requirement of Intercrop (Garlic) and T6 - Sugarcane [Fertilizer as per
sugarcane and a popular intercrop in non-mills zone for HYG] Intercrop (Garlic). The cultivated variety
area for high sugarcane and intercrops yield is very was Isd 38. The experiment was set up on Nov. 04,
important for sustainable sugarcane production. It is 2009 at Rangamati , at Chapai Nawabganj (Sibgang)
spectacular that the poor sugarcane and intercrops Nov. 10, 2009 and at Sirajganj Nov, 15, 2009, with
yield is largely attributed to improper use of fertilizer. three budded sett following conventional method.
The nutrient supplying capacity of soil is always Yield and yield attributes
changing for its nutrient mining due to intensive crop Yield and its components like tiller, millable cane yield
cultivation and some other manipulations. Thus it and Brix of cane were shown in Table 30, 31 and 32.
needs to update nutrient requirement for sugarcane Application of fertilizer showed significantly better in
and intercrops in non-mills zone area. A field respect of all parameters except brix per cent of
experiment was conducted at Sirajganj (AEZ 7), cane. Treatment T3- Sole sugarcane (Fertilizer as per
Chapai Nawabganj (AEZ 11), and Rangamati (AEZ STV for HYG) produced highest sugarcane yield of
29) to find out the nutrient requirement of sugarcane 115.2 t ha-1, 114.8 and 92.08 t ha-1 at Sirajganj,
and intercrops productivity in non-mills zone area and Rangamati and Shibganj, respectively (Table 35, 37
to update the fertilizer application of sugarcane under and 39). Incase of intercrop (Garlic) highest yield data
AEZ 7, AEZ 11 and AEZ 29 based on the nutrient (1.93 t ha-1) was recorded from T6 treatment at
management packages. The experiment was laid out Sirajganj and 4.06 t ha-1 at Shibganj (Table 30 and
in RCBD with three replications. The experiment 32).
comprised of six treatments: T1- Sole sugarcane
(Fertilizer as per FRG’05), T2- Sole sugarcane
(Fertilizer as per STV for MYG); T3- Sole sugarcane

Table 30. Site specific fertilizer requirement for sustainable production of sugarcane and intercrop in
non-mills zone (Sirajganj AEZ-7).

Treatments Tiller Millable Yield Brix Garlic Yield
(×103 ha-1) cane (t ha-1) (%) (t ha-1)
T1- Sole sugarcane (Fertilizer as per FRG’05) 15.30
T2- Sole sugarcane(Fertilizer as per STV for MYG) 158.6 bc (× 103 ha-1) 100.5 c 14.65 1.86
T3- Sole sugarcane (Fertilizer as per STV for HYG ) 151.6 bc 112.0 ab 109.5 ab 15.25 1.57
T4- Sugarcane [ Fertilizer as per FRG’05] + 162.4 b 115.2 a 15.78 1.93
157.8 bc 117.6 ab 80.83 d
Intercrop (Garlic) 14.73
T5- Sugarcane [Fertilizer as per STV for MYG] + 126.7 a
13.80
Intercrop (Garlic) 109.4 ab
T6- Sugarcane [Fertilizer as per STV for HYG]+ NS
180.3 a 98.84 b 106.3 bc
Intercrop (Garlic
LSD(5%) 149.7 C 110.0 ab 110.2ab

10.19 20.16 7.810

Table 31. Site specific fertilizer requirement for sustainable production of sugarcane and intercrop in
non-mills zone (Rangamati (AEZ-29).

Treatments Tiller Millable cane Yield Brix
(×103 ha-1) (× 103 ha-1) (tha-1) (%)
T1- Sole sugarcane (Fertilizer as per FRG’05) 118.9 b 112.8 ab 20.92
T2- Sole sugarcane(Fertilizer as per STV for MYG) 117.7 a 111.0 b 20.93
T3- Sole sugarcane (Fertilizer as per STV for HYG ) 129.1 a 121.0 a 114.8 a 21.18
T4- Sugarcane [ Fertilizer as per FRG’05] + Intercrop (Garlic) 130.9 a 116.1 a 103.1 c 20.91
T5- Sugarcane [Fertilizer as per STV for MYG]+ Intercrop (Garlic) 129.1 a 115.5 a 85.86 d 21.07
T6- Sugarcane [Fertilizer as per STV for HYG**]+ Intercrop (Garlic) 134.6 a 107.0 b 105.6 c 21.73
LSD(5%) 132.0 a 119.0 a 3.572

9.207 8.076

Soils & Nutrition Division 47

Table 32. Site specific fertilizer requirement for sustainable production of sugarcane and intercrop in
non-mills zone (Shibganj AEZ-7).

Treatments Tiller Millable cane Yield Brix Garlic Yield
(×103 ha-1) (× 103 ha-1) (t ha-1) (%) (t ha-1)
T1- Sole sugarcane (Fertilizer as per FRG’05) 82.41 ab 4.06
T2- Sole sugarcane(Fertilizer as per STV for MYG) 108.1 70.86 c 80.77 ab 19.64 bc
T3- Sole sugarcane (Fertilizer as per STV for HYG) 90.00 92.65 a 92.08 a 19.12 c
T4- Sugarcane [ Fertilizer as per FRG’05] + 123.9 82.23 b 72.41 b 20.72 a
108.5 94.23 a 20.36ab
Intercrop (Garlic) 78.69 ab
T5- Sugarcane [Fertilizer as per STV for MYG]+ 107.5 81.18 b 20.74 a 3.82
84.07 ab
Intercrop (Garlic) 98.62 91.25 a 19.96 abc 3.92
T6- Sugarcane [Fertilizer as per STV for HYG**]+ 13.50
NS 3.435 0.9712
Intercrop (Garlic
LSD(5%)

Soil nutrient status after sugarcane harvest
Slight increasing tendency was observed in case of P & K over initial soil, some cases S also increase other
nutrients remained unchanged (Table 33).

Table 33. Status of initial and post harvest soil for sustainable production of sugarcane and intercrop
in non-mills zone (Sirajganj AEZ-7)

Treatments Analytical value
Initial soil
Post harvest pH OC (%) Total N Avail. P Exch. K (meq%) Avail. S
T1 (%) (ppm) (ppm)
T2
T3 6.10 0.86 0.05 6.5 0.11 12.0
T4
T5 6.62 0.64 0.060 7.0 0.12 7.0
T6 6.60 0.64 0.060 7.0 0.12 8.0
6.64 0.75 0.055 9.0 0.13 12.5
6.63 0.65 0.050 10.0 0.12 12.0
6.63 0.67 0.060 10.0 0.12 10.5
6.54 0.67 0.050 7.0 0.12 14.0

Soil nutrient status after sugarcane harvest
Slight increasing tendency was observed in case of P & S but K was decrease over initial soil, other nutrients
remained unchanged (Table 34).

Table 34. Status of initial and post harvest soil for sustainable production of sugarcane and intercrop
in non-mills zone (Rangamati (AEZ-29).

Treatments Analytical value
Initial soil
Post harvest pH OC (%) Total N Avail. P Exch. K (meq%) Avail. S
T1 (%) (ppm) (ppm)
T2
T3 5.70 0.73 0.06 7.0 0.16 12.0
T4
T5 5.54 0.67 0.060 12.0 0.12 12.0
T6 5.37 0.81 0.055 14.0 0.12 15.0
5.56 0.88 0.070 12.0 0.12 15.5
5.55 0.85 0.060 12.0 0.12 15.0
5.43 0.81 0.060 12.0 0.10 15.0
5.49 0.78 0.055 11.0 0.10 14.0

48 Annual Report, 2010-2011

Soil nutrient status after sugarcane harvest
There are slight decreasing tendency was observed in case of K over initial soil, other nutrients remained
unchanged (Table 35).

Table 35. Status of initial and post harvest soil for sustainable production of sugarcane and intercrop
in non-mills zone (Shibganj AEZ-7).

Treatments Analytical value
Initial soil
Post harvest pH OC (%) Total N Avail. P Exch. K (meq%) Avail. S
T1 (%) (ppm) (ppm)
T2
T3 7.03 0.98 0.05 21.0 0.41 15.0
T4
T5 7.03 0.99 0.050 19.0 0.38 15.0
T6 7.00 0.96 0.060 25.0 0.33 10.5
7.06 0.99 0.060 19.0 0.38 13.0
7.05 1.13 0.060 25.0 0.36 10.0
6.98 0.99 0.055 25.0 0.38 10.5
7.02 0.96 0.050 21.0 0.35 10.0

SILICON FERTILIZATION ON SUGARCANE IN (AEZ-1) during 2009-2010 cropping season. The
TWO DIFFERENT SOILS: EFFECTS ON YIELD experiment was set up on Dec. 24, 2009 at BSRI
CONTRIBUTING PARAMETERS, LEAF Farm and Jan. 3, 2010 at RSRS Thakurgoan in pit
NUTRIENT CONCENTRATIONS AND SOIL method (2 budded 12 setts per pit). The experiments
PROPERTIES were laid out in CRD with six replications. Twelve
numbers of two budded sett was sown in each pit.
Silicon (Si) is one of the most abundant elements in The experiment comprised of six treatments: T1-N, T2-
the lithosphere and most soils contain considerable N+P, T3- N+P+K, T4- N+P+K+S, T5 - N+P+K+S+Zn,
amounts of it. It is accumulated in plants at a level T6- N+P+K+S+Zn+ Si. The N, P, K, S and Zn fertilizer
equivalent to that of macronutrient elements such as were applied in each pit following our recommended
calcium, magnesium and phosphorous. It is also doses. CaSiO3 was used as the source of Si and
ubiquitous in plants and its effects include applied @ 6000 kg ha-1.
enhancement of growth and increasing resistance to
drought stress, salinity and toxic metals. Yield and yield attributes
Nevertheless, continual cropping can deplete levels Yield and its components like tiller, millable cane,
of Si available to the crop, making application of Si- yield and Brix of cane were shown in Table 44 & 46.
containing fertilizers necessary to maintain crop There was no significant effect found in respect of all
productivity. parameters at BSRI farm but at RSRS Thakurgoan
The experiment was conducted at Bangladesh application of silicon fertilizer (T6) produced highest
Sugarcane Research Institute experimental farm sugarcane yield of 16 kg per pit which was
located in Ishurdi series under High Ganges River statistically significant with other treatments, other
Floodplain soils (AEZ 11) and another location was at parameters were statistically identical. (Table 36 &
Regional sugarcane Research Station farm (RSRS), 38).
Thakurgoan under Old Himalayan Piedmont Plain

Table 36. Effect of silicon on sugarcane yield and yield contributing parameters at BSRI Farm.

Treatment Tiller Millable cane Yield Brix
(per pit) (per pit) (Kg pit-1) (%)
T1 - N as per RFD
T2 - N &P as per RFD 20 16.50 28.00 15.90
T3 - N ,P & K as per RFD 25 16.83 28.17 15.32
T4 - N,P,K&S as per RFD 19 17.17 29.50 14.93
T5 - N,P,K,S&Zn as per RFD 20 16.83 26.83 15.92
T6- N,P,K,S,Zn & Si as per RFD
LSD(5%) 20 16.33 26.67 15.55
22 17.33 28.50 16.25
NS
NS NS NS

Soils & Nutrition Division 49

Soil nutrient status after sugarcane harvest
The nutrient status after sugarcane harvest remained unchanged (Table 37).

Table 37. Status of initial and post harvest soil in the Experiment at BSRI farm.

Treatments Analytical value
Initial soil
Post harvest pH OC (%) Total N Avail. P Exch. K Avail. S
T1 (%) (ppm) (meq%) (ppm)
T2
T3 7.6 0.72 0.11 8.0 0.17 19.0
T4
T5 7.67 0.76 0.11 8.50 0.17 14.50
T6 7.71 0.74 0.11 6.30 0.17 17.33
7.72 0.84 0.11 6.30 0.18 17.50
7.66 0.64 0.11 6.50 0.18 18.80
7.68 0.78 0.10 6.17 0.16 19.00
7.65 0.84 0.11 6.00 0.17 18.50

Table 38. Effect of silicon on sugarcane yield and yield contributing parameters at RSRS Thakurgaon.

Treatment Tiller Millable cane Yield Brix
T1- N as per RFD (per pit) (per pit) (Kg pit-1) (%)
T2- N &P as per RFD
T3- N ,P & K as per RFD 14 13.33 13.00 b 18.42
T4- N,P,K&S as per RFD 15 12.33 11.00 b 18.33
T5- N,P,K,S&Zn as per RF D 15 12.67 11.42 b 18.33
T6- N,P,K,S,Zn & Si as per RFD 13 12.67 11.08 b 18.55
LSD(5%) 16 12.17 11.25 b 18.65
16 14.00 16.00 a 18.28
ns
ns 2.502 ns

Soil nutrient status after sugarcane harvest
The nutrient status after sugarcane harvest remained unchanged (Table 39).

Table 39. Status of initial and post harvest soil in the Experiment at BSRI farm.

Treatments Analytical value

Initial soil pH OC (%) Total N Avail. P Exch. K (meq%) Avail. S
Post harvest (%) (ppm) (ppm)
T1 35.0
T2 5.15 1.60 0.09 42.0 0.15
T3 32.0
T4 4.95 1.36 0.09 40.0 0.13 35.0
T5 5.05 1.40 0.10 38.0 0.15 36.0
T6 5.00 1.38 0.08 35.0 0.14 38.0
5.10 1.45 0.10 41.0 0.15 35.0
5.12 1.42 0.09 40.0 0.15 35.0
5.10 1.52 0.09 38.0 0.14

50 Annual Report, 2010-2011

PHYSIOLOGY AND SUGAR CHEMISTRY DIVISION

RESEARCH HIGHLIGHTS The highest germination per cent was recorded
The clones I 231-03 and I 124-00 were recorded from the clone I 124-00 (68 %) in 1st November
as highly tolerant under field drought stress batch.
condition at both Godagari (Rajshahi) and Effects of light interception on sequential
Patuadangi farm of RSRS (Thakurgaon). These intercrop production in sugarcane was
clones produced highest cane yield at Godagari investigated that the treatment combinations of
(Rajshahi) location compared to RSRS V1S3C1 (variety Isd 37, row to row spacing 120
(Thakurgaon) location. cm, potato-mungbean with sugarcane leaf
The clones I 231-03 was recorded as highly clipping) is better than other treatment
tolerant at both induced and natural water-logging combinations.
and flood stress conditions.
The clones I 231-03 was recorded as highly stage were recorded phosphate content at
tolerant under salinity stress conditions. desired level.
Granular gur production, packaging and
SUGAR CHEMISTRY SECTION preservation techniques have been developed.
Date palm syrup having 72-78% Brix can be
The clones I 112-01 and I 137-03 under ZYT-III preserved successfully throughout the year for
and the Clones I 7-03, I 39-04 & I 189-04 under consumption.
ZYT-II were early maturing potential. The varieties Isd 37 and Isd 34 were found similar
The clones I 124-00, I 78-03, I 137-03 and in respect of cane quality both in high land and
I 231-03 under ZYT-III and I 07-03 & I 284-04 water-logg condition than the variety Isd 36
under ZYT-II test stages were found suitable for The qualities of cane crushed by the gur makers
gur making. in the mills zones (NBSM ) were superior to that
The clones I 112-01 (350 ppm), I 78-03 (363 crushed by the sugar mills.
ppm), I 137-03 (383 ppm), I 231-03 (380 ppm),
under ZYT-III test stage; I 07-03 (350 ppm),
I 6-04 (415 ppm), I 189-04 (358 ppm), & I 284-04
(368 ppm) under ZYT-II test stage and I 25-04
(393 ppm), I 150-05 (357 ppm), Isd 18 T1 (329
ppm) & Isd 18 T2 (358 ppm) under ZYT-I test

PHYSIOLOGY SECTION 00 were I 231-03 recorded as highly tolerant at
DROUGHT TOLERANCE IN SUGARCANE Godagari(Rajshahi) location (Table 3). The clone I
An investigation was carried out with advanced 231-03 gave the highest yield (72.3 t.ha-1) followed by
clones during 2009-10 cropping season at BSRI (pot), clone I 124-00 (71.6 t.ha-1) at RSRS (Thakurgaon)
Patuadangi farm of Regional Sugarcane Research location while the highest yield (83.1 t.ha-1) was
Station (RSRS), Thakurgaon and at Godagari obtained in clone I 231-03 followed by clone I 124-00
(Rajshahi) farmer’s field. Results of the investigation (81.6 t.ha-1) at Godagari(Rajshahi) location. It is also
have been presented in the Tables 1, 2 and 3. It is seen that relatively poor yield was recorded at RSRS
seen that clones I 231-03 shows better CSI than (Thakurgaon) location compare to Godagari
other clones and were found highly tolerant at BSRI (Rajshahi) location possibly due to poor soil fertility
(pot) location (Table 1), the clones I 124-00 and I level at RSRS (Thakurgaon) location. Brix per cent
231-03 were found highly tolerant at RSRS was recorded at RSRS (Thakurgaon) location and
(Thakurgaon) location (Table 2), while clones I 124- presented in Table 2.

51

Table 1. Total Chlorophyll, CSI, Proline and Grading performance of BSRI bred clones under drought
stress conditions (PVC Pipe experiment).

Varieties/ clones Total Chlorophyll CSI Proline Grading
I 124-00 (mg/g) 75.2 (mg/g) (1-5)*
I 112-01 1.73 63.3 0.561 2
I 7-03 1.31 61.2 0.445 3
I 78-03 1.23 61.7 0.438 4
I 111-03 1.01 65.6 0.441 4
I 137-03 1.23 73.3 0.448 3
I 139-03 1.26 60.3 0.450 3
I 231-03 1.22 81.6 0.435 4
Isd 34 1.85 80.2 0.982 1
Isd 39 1.77 79.7 0.621 1
Isd 40 1.93 80.1 1.520 1
1.95 0.661 1

Table 2. Screening sugarcane clones against drought stress (Location: RSRS, Thakurgaon).

Varieties / Clones Tillers Millable cane Yield Brix (%) Grading
ZYT-III (103 ha-1) (103 ha-1) (t ha-1) (1-5)*
I 124-00 92.3 71.6 19.1 1
I 112-01 128.0 72.3 55.6 18.0 3
I 7-03 121.1 73.0 48.7 17.1 4
I 78-03 115.3 71.4 69.3 17.7 2
I 111-03 110.4 80.4 69.2 17.5 2
I 137-03 125.7 74.1 54.9 18.5 3
I 139-03 127.1 83.8 70.1 15.8 2
I 231-03 121.3 95.6 72.3 17.5 1
ZYT-II 136.7 86.9 74.0 18.5 1
I 06-04 122.0 78.1 59.1 20.7 3
I 25-04 123.7 82.2 71.7 18.8 2
I 39-04 116.7 81.7 69.6 17.7 2
I 152-04 116.2 89.3 76.7 17.5 1
I 189-04 123.7 78.6 45.3 18.5 4
I 284-04 128.1 81.2 71.3 17.4 2
ZYT-I 125.6 75.6 62.0 16.4 2
I 38-05 129.8 71.8 52.1 16.8 3
I 91-05 125.6 72.9 69.4 18.2 2
I 94-05 115.8 86.3 77.6 17.3 2
I 139-05 130.8 82.0 61.8 17.6 3
I 150-05 114.8 85.3 72.3 16.0 2
Isd 18 (T2) 134.2 87.5 71.3 18.3 1
Isd 18 (T1) 129.7 81.8 70.5 16.1 2
Standard 112.9 71.2 62.6 16.7 3
Isd 20 132.4 78.3 70.3 18.6 2
Isd 32 118.2 79.2 74.7 19.7 1
Isd 33 115.1 88.4 70.0 19.0 2
Isd 36 134.0 87.9 75.9 18.8 1
Isd 37 134.7 82.9 75.3 18.4 1
Isd 38 131.6
Isd 39
Isd 40

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly tolerant.

52 Annual Report, 2010-2011

Table 3. Screening sugarcane clones against drought stress (Location: Godagari, Rajshahi).

Varieties / Clones Tillers Millable cane Yield Grading
ZYT-III (103 ha-1) (103 ha-1) (t ha-1) (1-5)*
I 124-00 94.5 81.6 1
I 112-01 134.6 81.2 63.9 3
I 7-03 119.4 70.0 52.3 3
I 78-03 116.7 65.3 50.6 3
I 111-03 108.5 73.3 59.1 3
I 137-03 116.3 85.8 53.6 3
I 139-03 116.5 91.4 71.7 2
I 231-03 127.4 96.2 83.1 1
ZYT-II 139.6 97.3 82.9 1
I 06-04 115.4 72.7 66.5 3
I 25-04 114.9 110.0 103.7 1
I 39-04 156.6 81.6 68.7 3
I 152-04 117.3 102.4 93.0 1
I 189-04 144.8 85.9 75.2 2
I 284-04 139.6 92.6 77.5 1
Standard 118.4 87.5 74.2 2
Isd 20 115.2 74.3 63.2 3
Isd 32 127.6 83.5 72.6 2
Isd 33 112.3 85.1 78.5 1
Isd 36 119.7 83.7 73.1 2
Isd 37 139.8 95.4 84.2 1
Isd 38 139.2 91.7 82.4 1
Isd 39 128.9
Isd 40

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected where 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly tolerant.

WATER-LOGGING TOLERANCE IN SUGARCANE Water-logging stress trial conducted at farmer’s field
Trials were conducted at BSRI (pot), BSRI farm and (Lalpur location) clones I 231-03 showed highly
farmer’s field with BSRI bred advanced clones under tolerant reaction against 90 d water-logging stress
both induced and natural water-logging stress period and gave 84.2 t.ha-1 estimated cane yield
conditions during 2009-10 cropping season. Results (Table 11). In case of trial carried out at farmer’s field
have been presented in the Tables 4-14. At pot trial (Sirajgonj location) clone I 231-03 showed highly
clones I 231-03 showed highly tolerant reaction upto tolerant reaction against 90 d water-logging stress
120 d water-logging stress period having 43.7 per period and gave estimated cane yields 92.2 t.ha-1
cent green leaves (Table 4). The highest primordial (Table 12). The trial carried out at farmer’s field
water roots were recorded in clone I 231-03. (Table (Jamalpur location) clones I 231-03, I 6-04, I 25-04, I
5). The highest total chlorophyll content was recorded 39-04 and I 189-04 showed highly tolerant reaction
in clone I 231-03 (Table 6). Growth rate ranged in against 90 d water-logging stress period and gave
0.34-0.82 cm/day under water-logging stress estimated cane yields 94.3t.ha-1, 98.6 t ha-1, 95.2 t ha-
condition 90 d (Table 7) pot expt. In case of field trial 1, 81.8 t ha-1 and 85.1 t ha-1, respectively (Table 13).
conducted at BSRI farm clones I 231-03 was The trial carried out at farmer’s field (Atwari,
recorded as highly tolerant upto 120 d water-logging Panchaghar location) clones I 124-00, I 231-03, I 6-
stress period (Table 8) having 33.1 per cent green 04 and I 89-04 showed highly tolerant reaction
leaves. In this trial the highest estimated cane yield against 90 d water-logging stress period and gave
(94.5 t.ha-1) was obtained in clone I 231-03 having estimated cane yields, 74.0 t ha-1, 75.7 t ha-1, 77.8 t
13.8 Pol% cane (Table 10). ha-1 and 78.6 t ha-1, respectively (Table 14).

Physiology & Sugar Chemistry Division 53

Table 4. Performance of BSRI bred sugarcane clones under induced water logging stress condition
(BSRI, Pot experiment).

water logging stress period (Days) 120 Tolerance
Dry leaf Green leaf rating scale
Varieties / 30 60 90
Clones Dry leaf Green leaf (%)/ (%)/Plant (1-5)*
Dry leaf Green leaf Dry leaf Green leaf Plant
ZYT - III (%)/ (%)/ Plant (%)/ (%)/ (%)/ (%)/Plant
I 124-00 Plant Plant Plant Plant
I 112-01
I 7-03 38.7 61.3 47.2 52.8 58.8 41.2 67.6 32.4 2
I 78-03 41.3 58.7 50.7 49.3 67.1 32.9 73.3 26.7 3
I 111-03 40.4 59.6 51.3 48.7 66.6 33.4 74.8 25.2 3
I 137-03 41.8 58.2 48.6 51.4 60.9 39.1 71.1 28.9 3
I 139-03 47.6 52.4 58.8 41.2 69.4 30.6 79.7 20.3 4
I 231-03 38.5 61.5 46.9 53.1 56.9 43.1 68.9 31.1 2
ZYT- II 42.9 57.1 61.6 38.4 70.8 29.2 78.4 21.6 4
I 6-04 38.8 61.2 44.7 55.3 48.7 51.3 56.3 43.7 1
I 25-04
I 39-04 40.7 59.3 46.9 53.1 57.2 42.8 69.2 30.8 2
I 152-04 39.3 60.7 43.8 56.2 46.8 53.2 58.5 41.5 1
I 189-04 47.2 52.8 49.7 50.3 61.9 38.1 75.1 24.9 3
I 284-04 42.4 57.6 50.8 49.2 62.6 37.4 69.8 30.2 2
ZYT- I 37.5 62.5 51.9 48.1 58.8 41.2 61.4 38.6 1
I 38-05 41.7 58.3 53.6 46.4 60.5 39.5 69.9 30.1 2
I 91-05
I 94-05 42.9 57.1 50.4 49.6 56.1 43.9 68.7 31.3 2
I 139-05 40.6 59.4 51.8 48.2 59.7 40.3 67.3 32.7 2
I 150-05 40.3 59.7 46.9 53.1 52.2 47.8 60.6 39.4 1
Isd 18 (T1) 39.9 60.1 47.7 52.3 54.8 45.2 69.1 30.9 2
Isd 18 (T2) 45.1 55.4 56.9 43.1 61.4 38.6 67.5 32.5 2
Standard 45.9 54.9 58.8 41.2 68.7 31.3 75.4 24.6 3
Isd 39 41.0 59.0 57.5 42.5 63.9 36.1 74.9 25.1 3
Isd 40
37.3 62.7 44.1 55.9 55.8 44.2 62.1 37.9 1
41.3 58.7 48.6 51.4 56.4 43.6 64.8 35.2 1

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly intolerant.

Table 5. Performance of BSRI bred sugarcane clones under induced water logging stress condition
(BSRI, pot experiment).

Varieties / Clones Fresh weight of PWR/Plant (g) Dry weight of PWR/Plant (g) Volume of PWR/Plant (cc)
ZYT-III 20.0
I 124-00 11.5 3.9 25.0
I 112-01 17.7 3.9 14.3
I 7-03 10.4 3.4 17.1
I 78-03 9.1 2.1 21.0
I 111-03 28.3 4.1 11.0
I 137-03 6.2 2.3 21.9
I 139-03 14.3 3.5 65.0
I 231-03 57.7 10.5 88.0
ZYT-II 25.
I 6-04 74.4 17.4 25.8
I 39-04 16.8 4.7 44.2
I 152-04 17.7 5.1 103.3
I 189-04 36.2 8.8 90.0
I 25-04 89.7 23.5 115.0
I 284-04 74.2 14.4 42.1
ZYT-I
I 38-05 105.5 20.4
I 91-05 35.6 6.9

54 Annual Report, 2010-2011

Varieties / Clones Fresh weight of PWR/Plant (g) Dry weight of PWR/Plant (g) Volume of PWR/Plant (cc)
I 94-05 32.5
I 139-05 21.3 3.8 58.3
I 150-05 33.2 6.3 59.2
Isd 18 (T1) 39.8 8.0 110.0
Isd 18 (T2) 101.4 17.9 66.7
Standard 63.0 11.1
Isd 34
Isd 39 76.0 12.2 86.0
Isd 40 87.6 19.2 98.0
87.1 16.9 92.6

Table 6. Total Chlorophyll, Chlorophyll-a, Chlorophyll-b, and Chlorophyll “a/b” ratio of BSRI bred
sugarcane clones under induced water-logging stress condition (BSRI, Pot experiment).

Varieties/ Total chlorophyll Chlorophyll-a Chlorophyll-b Chlorophyll “a/b” ratio
Clones (mg g-1) (mg g-1) (mg g-1)
3.00
I 124-00 1.85 1.34 0.44 3.00
I 112-01 1.49 1.03 0.34 3.37
I 7-03 1.51 1.17 0.32 3.36
I 78-03 1.48 1.11 0.33 3.38
I 111-03 1.38 1.05 0.31 3.37
I 137-03 1.36 0.98 0.29 3.48
I 139-03 1.37 1.01 0.29 2.51
I 231-03 1.92 1.31 0.52 3.38
Isd 34 1.89 1.32 0.39 2.76
Isd 39 1.91 1.38 0.49 2.85
Isd 40 1.90 1.37 0.48

Table 7. Growth rate of BSRI bred sugarcane clones under induced water logging stress condition
(BSRI, Pot experiment).

Varieties / Clones Growth rate (cm/day)
ZYT-III 30 60 90
I 124-00
I 112-01 0.53 0.43 0.38
I 7-03 0.91 1.08 0.72
I 78-03
I 111-03 0.5 0.41 0.38
I 137-03 0.80 0.75 0.60
I 139-03 0.66 0.61 0.52
I 231-03 0.93 0.73 0.52
ZYT-II 1.36 1.13 0.68
I 6-04 1.06 0.95 0.64
I 25-04 0.93 0.85 0.62
I 39-04 0.86 0.80 0.57
I 152-04 0.80 1.25 0.76
I 189-04
I 284-04 1.4 1.31 0.79
ZYT-I 1.5 1.16 0.77
I 38-05 1.2 0.95 0.82
I 91-05 0.93 0.81 0.59
I 94-05 1.1 0.86 0.65
I 139-05 0.9 0.71 0.54
I 150-05 0.85 0.80 0.60
Isd 18 (T1) 0.86 0.66 0.53
Isd 18 (T2) 0.63 0.41 0.38
Standard 0.46 0.40 0.34
Isd 34 1.13 0.55 0.64
Isd 39 1.63 1.26 0.77
Isd 40 0.66 0.38 0.40

Physiology & Sugar Chemistry Division 55

Table 8. Performance of BSRI bred sugarcane clones under induced water-logging stress condition
(BSRI farm).

Varieties / Water logging stress period (Days) Tolerance
Clones 30 60 90 120 rating scale

Dry leaf (%) Green leaf Dry leaf (%) Green leaf Dry leaf (%) Green leaf Dry leaf (%) Green leaf (1-5)*
/ Plant (%) / Plant / Plant (%) / Plant / Plant (%) / Plant / Plant (%)/ Plant

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where
1 = highly tolerant, 2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly intolerant.

Table 9. Growth rate of BSRI bred sugarcane clones under induced water logging stress condition
(BSRI, farm).

Varieties / Clones Growth rate (cm/day)
ZYT - III 30 60 90
I 124-00
I 112-01 0.63 0.37 0.53
I 7-03 0.67 1.02 0.89
I 78-03 0.89 0.63 0.62
I 111-03 1.34 1.34 1.09
I 137-03 1.91 1.09 0.94
I 139-03 1.27 1.00 0.92
I 231-03 0.23 0.26 0.37
ZYT –II 1.00 0.68 0.86
I 6-04
I 25-04 1.97 1.46 1.26
I 39-04 2.24 1.63 1.35
I 152-04 2.10 1.42 1.22
1.17 0.84 0.83

56 Annual Report, 2010-2011

Varieties / Clones Growth rate (cm/day)
I 189-04 30 60 90
I 284-04 1.19 0.88 0.84
ZYT- I 1.72 1.17 0.99
I 38-05
I 91-05 1.30 1.30 1.10
I 94-05 1.20 1.10 1.00
I 139-05 1.70 1.30 1.13
I 150-05 1.48 1.10 1.03
Isd18(T1) 1.32 1.19 1.06
Isd18 (T2) 1.02 0.79 0.78
Standard 0.49 0.49 0.52
Isd 20
Isd 34 1.29 0.97 0.89
Isd 37 1.42 0.93 0.99
Isd 38 2.42 1.63 1.37
Isd 39 1.82 1.29 1.29
Isd 40 1.82 1.28 1.67
1.06 0.92 0.92

Table 10. Performance of BSRI bred sugarcane clones under Induced water-logging stress condition
(BSRI, field).

Varieties / Clones Tillers Millable cane Yield Pol % cane
ZYT-III (103 ha-1) (103 ha-1) (t ha-1)
I 124-00 91.2 76.5 14.7
I 112-01 121.2 83.5 65.6 13.8
I 7-03 119.6 80.1 61.8 13.9
I 78-03 121.6 86.2 63.5 12.1
I 111-03 120.9 72.8 51.8 13.8
I 137-03 125.3 76.8 53.0 13.7
I 139-03 115.3 78.5 54.5 12.5
I 231-03 118.2 95.9 94.5 13.8
ZYT-II 137.7 97.1 87.8 12.4
I 6-04 140.3 95.6 85.6 13.5
I 25-04 137.9 82.4 67.2 11.9
I 39-04 128.2 97.6 79.6 11.7
I 152-04 139.7 85.3 79.1 12.3
I 189-04 114.7 96.2 72.9 13.9
I 284-04 128.8 95.3 78.4 12.3
ZYT-I 132.6 97.2 83.9 13.3
I 38-05 133.2 94.4 81.4 11.9
I 91-05 141.5 83.2 76.6 11.5
I 94-05 112.9 92.1 72.8 11.5
I 139-05 129.1 79.7 68.9 12.1
I 150-05 119.1 90.6 81.4 12.5
Isd18(T1) 121.8 96.5 78.6 14.5
Isd18 (T2) 123.5 112.7 89.0 13.2
Standard 149.6 84.7 81.8 13.7
Isd 20 122.1 92.1 78.6 14.5
Isd 34 132.6 99.7 98.0 14.0
Isd 37 124.4 97.4 95.2 14.1
Isd 38 122.6
Isd 39
Isd 40

Physiology & Sugar Chemistry Division 57

Table 11. Screening sugarcane against water logging stress (Location: Lalpur, Natore).

Varieties/Clones Tillers Millable cane Yield Grading
I 124-00 (103 ha-1) (103 ha-1) (t ha-1) (1-5)*
I 112-01 103.6 74.3 2
I 7-03 133.5 86.5 67.4 3
I 78-03 142.7 97.3 63.5 3
I 111-03 136.8 105.6 64.0 3
I 137-03 138.9 104.2 65.8 3
I 139-03 141.3 93.8 56.4 4
I 231-03 144.8 107.7 62.4 3
I 6-04 156.9 125.7 84.2 1
I 25-04 155.2 128.1 87.7 1
I 39-04 165.6 97.6 62.5 3
I 152-04 148.4 110.8 63.1 3
I 189-04 150.0 116.3 77.2 2
I 284-04 144.1 125.0 73.8 2
Isd 37 159.7 107.6 69.5 2
Isd 38 148.3 102.4 88.4 1
Isd 39 135.4 98.5 65.6 2
Isd 40 161.5 106.6 84.1 1
169.2 1034 81.3 1
167.4

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly tolerant.

Table 12. Screening sugarcane against water logging stress. (Location: Sirajgonj).

Varieties / Clones Tillers Millable cane Yield Grading
I 124-00 (103 ha-1) (103 ha-1) (t ha-1) (1-5)*
I 112-01 98.2 77.3 2
I 7-03 128.4 92.2 74.6 2
I 78-03 115.6 89.3 65.2 3
I 111-03 124.2 84.7 63.4 3
I 137-03 115.5 87.8 68.5 3
I 139-03 121.2 85.5 67.2 3
I 231-03 119.4 96.1 76.5 2
I 6-04 121.5 102.6 92.2 1
I 25-04 134.6 105.8 89.4 1
I 39-04 132.3 92.3 76.7 2
I 152-04 143.8 89.2 62.6 3
I 189-04 128.3 91.5 74.2 2
I 284-04 135.2 88.3 75.9 2
Isd 31 128.7 84.8 73.4 2
Isd 34 141.3 81.5 61.8 2
Isd 37 128.5 115.7 85.2 1
Isd 38 156.4 98.3 88.6 1
Isd 39 129.6 93.7 75.3 2
Isd 40 134.3 108.2 89.8 1
147.5 99.8 83.4 1
132.7

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected where 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly tolerant.

58 Annual Report, 2010-2011

Table 13. Screening sugarcane against water logging stress (Location : Jamalpur).

Varieties/ Clones Tillers Millable cane Yield Grading
I 124-00 (103 ha-1) (103 ha-1) (t ha-1) 2
I 112-01 90.3 82.5 2
I 7-03 131.3 105.0 79.3 3
I 78-03 157.3 88.3 3
I 111-03 115.0 80.7 70.7 4
I 137-03 117.3 78.2 67.2 3
I 139-03 114.8 84.0 54.6 4
I 231-03 145.0 89.0 62.5 1
I 6-04 173.3 109.3 58.1 1
I 25-04 176.4 109.7 94.3 1
I 39-04 166.2 112.6 98.6 1
I 152-04 154.8 105.0 95.2 2
I 189-04 154.7 92.3 81.8 1
I 284-04 157.0 90.7 87.3 2
Isd 34 142.7 88.3 85.1 1
Isd 37 168.5 104.0 89.5 1
Isd 39 166.4 112.1 82.7 1
Isd 40 137.7 110.7 86.5 1
158.5 105.0 89.3
148.2 85.8

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where
1 = highly tolerant, 2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly tolerant.

Table 14. Screening sugarcane clones against water- logging stress (Location: Atwari, Panchaghar).

Varieties / Clones Tillers Millable cane Yield Brix (%) Grading
I 124-00 (103 ha-1) (103 ha-1) (t ha-1) 17.9 (1-5)*
I 112-01 95.2 16.6 2
I 78-03 148.3 91.4 74.0 15.5 3
I 137-03 137.4 79.8 68.9 17.3 3
I 231-03 144.0 86.4 65.6 15.8 3
I 6-04 131.1 94.4 62.8 18.9 1
I 39-04 139.9 96.4 75.7 18.3 1
I 152-04 154.2 106.4 77.8 17.2 2
I 189-04 144.8 91.0 72.2 18.0 2
I 7-03 152.9 111.1 66.0 17.2 1
I 111-03 151.9 87.8 78.6 17.8 3
I 139-03 123.8 89.9 56.7 16.4 3
Isd 20 136.6 90.0 63.6 18.6 2
Isd 32 141.7 94.9 65.7 16.9 1
Isd 34 158.3 91.1 80.0 18.9 2
Isd 37 148.2 120.3 73.0 18.5 1
Isd 39 162.0 94.3 76.0 18.7 1
Isd 40 147.7 102.9 82.2 17.8 1
151.0 92.0 85.0 1
159.2 81.5

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly tolerant.

FLOOD TOLERANCE IN SUGARCANE clones I 231-03, I 152-04, I 189-04, I 38-05, I 150-05
Trials were carried out at both pot and farmer’s field and the varieties Isd 34, Isd 39 & Isd 40 were
conditions covering both induced and natural flood recorded highly tolerant during induced flood water
stress condition during 2009-10 cropping season. stress having 33.9 to 44.0 per cent green leaves
Results of the trials have been presented in the (Table 15). It is seen from the Table 16 that the
Tables 15-20. In case of pot trial, different clones variety Isd 39 produced the highest quantity of
were subjected to flood water stress upto 120 d and primordial water roots followed by clones I 231-03,
and I 38-05. The clones I 231-03, I 124-00 and the

Physiology & Sugar Chemistry Division 59

varieties Isd 39, Isd 40 & Isd 34 showed better 93.2, 98.8 and 89.2 t.ha-1 estimated cane yield
chlorophyll level under induced flood stress condition. respectively (Table 19). The highest Pol per cent was
The clones I 112-01, I 231-03, I 152-04, I 150-05, Isd found in clones I 139-03 and I 152-04 (Table 19). In
39 and Isd 40 showed better growth under flood case of Chunarughat location I 231-03, I 6-04 and
stress up to 90 d. (Table 17). In case of trial the varieties Isd 32, Isd 39 and Isd 40 showed highly
conducted at farmer’s field (Paksey location) clones I tolerant reaction against natural flood stress and
231-03, I 6-04 and Isd 34 showed highly tolerant obtained 88.5, 91.2, 85.4, 82.7 & 84.6 t.ha-1
reaction against natural flood stress and obtained respectively (Table 20).

Table 15. Performance of BSRI bred sugarcane clones under induced Flood stress condition (Pot
experiment).

Varieties / 30 Flood stress period (Days) 120 Tolerance
Clones Dry leaf Green leaf 60 90 Dry leaf Green leaf rating scale
(%)/Plant (%)/plant Dry leaf Green leaf Dry leaf Green leaf (%)/plant (%)/plant
ZYT - III (%)/plant (%)/plant (%)/plant (%)/plant (1-5)*
I 124-00 25.1 74.9
I 112-01 24.2 75.8 34.3 65.7 34.6 65.4 69.2 30.8 2
I 7-03 23.0 77.0 37.3 62.7 47.8 52.2 68.5 31.5 2
I 78-03 39.4 60.6 44.6 55.4 61.6 38.4 80.3 19.7 4
I 111-03 36.6 63.4 54.9 45.1 60.2 39.8 76.8 23.2 3
I 137-03 22.2 77.8 46.0 54.0 59.4 40.6 78.3 21.7 3
I 139-03 30.9 69.1 42.9 57.1 56.5 43.5 80.7 19.3 5
I 231-03 30.1 69.9 45.0 55.0 51.6 48.4 59.0 41.0 2
ZYT- II 37.9 62.1 41.6 58.4 56.4 43.6 1
I 6-04 42.1 57.9
I 25-04 33.9 66.1 35.5 64.5 45.7 54.3 67.4 32.6 2
I 39-04 27.2 72.8 44.3 55.7 45.2 54.8 68.7 31.3 2
I 152-04 37.8 62.2 43.2 56.8 54.1 45.9 74.3 25.7 3
I 189-04 33.6 66.4 50.7 49.3 58.8 41.2 65.7 34.3 1
I 284-04 32.3 67.7 47.9 52.1 52.4 47.6 64.0 36.0 1
ZYT- I 38.4 61.7 53.9 46.1 69.3 30.7 2
I 38-05 29.5 70.5
I 91-05 39.5 60.5 39.3 60.7 42.9 57.1 56.0 44.0 1
I 94-05 20.4 79.6 48.0 52.0 56.5 43.5 70.3 29.7 2
I 139-05 30.9 67.1 31.0 69.0 64.0 36.0 74.3 25.7 3
I 150-05 33.8 66.2 45.0 55.1 51.6 48.4 69.0 31.0 2
Isd18(T1) 40.4 59.6 50.8 49.2 58.8 41.2 66.1 33.9 1
Isd18 (T2) 35.3 64.7 50.5 49.5 54.7 45.3 68.2 31.8 2
Standard 49.2 50.8 50.2 49.8 69.8 30.2 2
Isd 34 31.1 68.9
Isd 39 19.3 80.7 43.8 56.2 50.6 49.4 64.2 35.8 1
Isd 40 26.9 73.1 28.0 72.0 39.3 60.7 58.6 41.4 1
38.5 61.5 44.4 55.6 62.2 37.8 1

Table 16. Performance of BSRI bred sugarcane clones under induced flood stress condition (Pot
experiment).

Varieties / Clones Fresh weight of PWR/Plant (g) Dry weight of PWR/Plant (g) Volume of PWR/Plant (cc)
ZYT – III 278.6
I 124-00 222.0 73.3 64.0
I 112-01 30.2 21.9 40.8
I 7-03 27.0 4.8 67.1
I 78-03 41.9 9.8 57.1
I 111-03 50.4 10.3 21.7
I 137-03 17.3 5.1 66.4
I 139-03 48.6 9.3 640.0
I 231-03 570.0 116.7 356.0
ZYT- II 91.7
I 6-04 114.0 15.0
I 25-04 62.0 12.8

60 Annual Report, 2010-2011

Varieties / Clones Fresh weight of PWR/Plant (g) Dry weight of PWR/Plant (g) Volume of PWR/Plant (cc)
I 39-04 40.1 7.2 65.7
I 152-04 62.8 10.9 92.5
I 189-04 125.5 18.8 146.3
I 284-04 149.3 20.7 168.6
ZYT- I
I 38-05 600.0 99.2 637.5
I 91-05 36.3 6.3 56.7
I 94-05 45.2 7.4 58.3
I 139-05 94.4 12.8 105.0
I 150-05 85.6 12.3 95.0
Isd18(T1) 226.8 30.9 240.0
Isd18 (T2) 181.8 21.4 158.3
Isd 34 380.0 89.5 570.0
Isd 39 836.0 133.1 894.0
Isd 40 315.7 55.1 338.6

Table 17. Growth rate of BSRI bred sugarcane clones under induced flood stress condition (Pot
experiment).

Varieties / Clones Growth rate (cm/day)
ZYT-III 30 60 90
I 124-00 1.2 1.02 0.93
I 112-01 1.6 0.94 1.22
I 7-03 1.0 0.71 0.68
I 78-03 1.13 0.93 0.91
I 111-03 1.26 1.1 1.02
I 137-03 0.93 0.81 0.83
I 139-03 1.65 1.33 1.14
I 231-03 1.82 1.48 1.31
ZYT-II 1.02 1.19 1.11
I 6-04 1.62 1.35 1.21
I 25-04 1.5 1.29 1.14
I 39-04 1.72 1.43 1.27
I 152-04 1.73 1.41 1.3
I 189-04 1.33 1.15 1.1
I 284-04 0.93 1.1 1.2
ZYT-I 1.27 1.07 1.05
I 38-05 0.74 0.76 0.81
I 91-05 1.08 1.09 1.08
I 94-05 1.51 1.3 1.24
I 139-05 1.03 1.0 1.03
I 150-05 1.08 1.06 1.07
Isd 18 (T1) 1.58 1.19 1.1
Isd 18 (T2) 2.1 1.58 1.37
Standard 1.7 1.34 1.22
Isd 34
Isd 39
Isd 40

Physiology & Sugar Chemistry Division 61

Table 18. Performance of BSRI bred sugarcane clones under induced flood stress condition (Pot
experiment).

Varieties/ Clones Chlorophyll “a” Chlorophyll “b” Total Chlorophyll Chlorophyll “a/b” ratio
I 124-00 1.40 .40 1.80 3.5
I 112-01 1.03 .37 1.40 2.78
I 7-03 .98 .30 1.28 3.3
I 78-03 1.02 .32 1.34 3.19
I 111-03 .99 .28 1.25 3.5
I 137-03 1.01 .31 1.32 3.25
I 139-03 .96 .30 1.26 3.2
I 231-03 1.33 .58 1.91 2.29
Isd 34 1.41 .40 1.80 3.5
Isd 39 1.51 .45 1.96 3.35
Isd 40 1.42 .57 1.99 2.49

Table 19. Screening sugarcane against flood stress. (Location : Paksey).

Varieties/ Clones Tillers Millable cane Yield Pol% cane Grading
(103 ha-1) (103 ha-1) (t ha-1) (1-5)*

ZYT-III 157.8 90.7 82.9 13.41 2
I 112-01 177.8 91.1 78.7 12.45 4
I 7-03 172.9 109.6 81.3 12.36 2
I 78-03 148.9 105.3 71.6 11.96 3
I 111-03 140.9 97.8 70.4 11.42 3
I 137-03 142.2 98.9 72.0 14.5 3
I 139-03 193.3 102.0 93.2 13.1 1
I 231-03 1
ZYT-II 187.1 104.2 98.8 13.74 2
I 6-04 157.3 97.6 81.3 12.6 3
I 25-04 168.4 93.1 68.3 13.6 2
I 39-04 164.4 97.1 89.0 14.1 2
I 152-04 186.2 100.9 85.2 13.8 2
I 189-04 177.2 104.3 88.2 11.7 1
I 284-04 196.0 112.5 89.2 11.5 2
Isd 34 186.5 108.2 84.3 11.8 2
Isd 39 171.1 104.0 79.4 13.0
Isd 40

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where , 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly intolerant

62 Annual Report, 2010-2011

Table 20. Screening sugarcane against flood stress. (Location : Chunarughat).

Varieties/ Clones Tillers Millable cane Yield Brix (%) Grading
(103 ha-1) (103 ha-1) (t ha-1) (1-5)*
ZYT-III 17.8
I 124-00 118.5 96.5 76.1 21.0 2
I 112-01 105.2 84.3 71.0 22.3 2
I 7-03 102.6 74.9 67.4 19.6 3
I 78-03 127.5 82.2 58.8 19.9 3
I 231-03 139.5 90.8 88.5 21.6 1
ZYT-II 20.1
I 6-04 132.5 93.3 91.2 22.2 1
I 39-04 116.3 97.3 83.8 18.5 2
I 80-04 113.7 91.7 75.1 22.2 2
I 139-04 115.7 87.7 68.7 20.1 3
I 137-04 105.4 71.2 55.0 22.5 4
I 152-04 109.6 82.3 72.3 18.7 2
I 284-04 97.4 83.8 61.4 22.0 3
Isd 32 128.4 87.6 85.4 22.5 1
Isd 37 117.3 92.2 78.1 23.0 2
Isd 39 125.2 92.8 82.7 1
Isd 40 119.5 96.2 84.6 1

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where, 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly intolerant

SALINITY TOLERANCE IN SUGARCANE and Isd 39 (85.6 t ha–1) having tolerance rating 1
A trial was conducted at Terokhada (Khulna) with (Table 21). It was also observed that performance of
BSRI released sugarcane varieties such as Isd-33, a variety depends on date of planting and level of
Isd 38, Isd 39, Isd 40 as well as BSRI bred clones management.
under ZYT II & ZYT III during 2009-10 cropping
season. The highest cane yield (85.7 t ha–1) was
obtained in I 231-03 followed by I 6-04 (82.7 t ha–1)

Physiology & Sugar Chemistry Division 63

Table 21. Screening sugarcane against Salinity stress. (Location : Terokhada, Khulna).

Varieties/ Tillers Millable cane Yield Grading
Clones (103 ha-1) (103 ha-1) (t ha-1) (1-5)*
94.4 82.6 2
ZYT-III 127.4 84.1 78.3 2
I 124-00 136.0 70.4 54.6 3
I 112-01 101.9 93.7 67.5 3
I 7-03 135.8 63.0 47.3 4
I 78-03 118.0 78.9 55.2 3
I 111-03 120.0 81.9 69.5 3
I 137-03 132.6 101.5 85.7 1
I 139-03 143.2 87.0 82.7 1
I 231-03 131.1 96.3 79.7 2
ZYT-II 131.1 85.9 75.1 2
I 6-04 135.6 94.8 73.9 2
I 39-04 139.3 70.0 56.0 4
I 152-04 111.5 73.0 69.4 3
I 189-04 131.2 85.6 76.2 2
I 284-04 119.9 78.1 85.6 1
Isd 33 117.0 92.2 80.7 2
Isd 38 134.3
Isd 39
Isd 40

* Tolerance rating scale (1-5) is based on greenness of plants and other data collected, where , 1 = highly tolerant,
2 = tolerant, 3 = moderately tolerant, 4 = intolerant and 5 = highly intolerant

SUGARCANE GERMINATION UNDER LOW presented in the Table 22. The highest germination
TEMPERATURE STRESS per cent was recorded in clone I 124-00 (68%)
An investigation was carried out to get the followed by clones I I 95-01 (66 %) in 1st November
germination potential of different advanced clones batch. The lowest germination per cent was recorded
during 2009-10 cropping season. Results have been in the batch planted on 15th January followed by
batch planted on 1st February (Table 22).

Table 22. Effects of ambient temperature on sugarcane germination.

Planting Date 01 Nov 15 Nov 01 Dec Germination (%) 01 Jan 15 Jan 01 Feb
Clones 68 51 57 15 Dec 44 43 37
I 124-00 66 64 53 56 44 42 49
I 95-01 62 57 54 46 47 28 38
I 112-01 59 57 58 37 38 34 40
I 145-02 58 54 48 44 55 47 41
I 191-02 47

EFFECTS OF LIGHT INTEREPTION ON The data of Tillers, millable cane, cane yield, Pol %
SEQUENTIAL INTERCROP PRODUCTION IN cane, intercrop yield, equivalent cane yield of
SUGARCANE intercrops and total adjusted cane yields were
The experiment was conducted at Bangladesh recorded and shown in Tables 23 and 24. The
Sugarcane Research Institute (BSRI) farm, Ishurdi, highest tiller and millable cane were obtained from
Pabna during 2009-10 cropping season with the the treatment V2S1C1 (Variety Isd 38, Spacing 80 cm,
sugarcane varieties Isd 37 and Isd 38. Potato and and sugarcane leaf clipping). The highest total
Mungbean were grown as first and second intercrops adjusted cane yield was recorded from the treatment
respectively. There were twelve treatment V1S3C1 (Variety Isd 37, Spacing 120 cm, Sugarcane
combinations in the trial. The experiment was set up leaf clipping). On the basis of totla adjusted cane
in RCB design with three replications. Cane yield the treatment combinations of V1S3C1 (Variety
cultivation practices were followed as per Isd 37, Row to Row spacing 120 cm, Potato -
recommendation. Mungbean with Sugarcane leaf clipping) is better than
other treatment combinations.

64 Annual Report, 2010-2011

Table 23. Effects of row to row spacing and sugarcane leaf clipping on tillers, millable canes, cane yield
and pol % cane.

Treatments Tillers Millable cane Cane yield Pol % cane
V1 S1C0 (103 ha-1) (103 ha-1) (t ha-1) 14.75
V1 S1C1 102.70 84.05 14.42
V1 S2C0 228.75 103.20 83.98 14.21
V1 S2C1 224.79 95.20 94.39 14.15
V1 S3C0 182.29 94.78 93.13 14.74
V1 S3C1 185.83 88.93 87.15 14.66
V2 S1C0 156.87 89.16 88.23 15.52
V2 S1C1 154.58 121.26 80.38 15.53
V2 S2C0 251.45 122.70 81.93 15.36
V2 S2C1 249.79 98.49 86.54 15.48
V2 S3C0 192.50 98.20 85.57 15.68
V2 S3C1 189.58 89.54 82.12 15.65
160.20 90.66 83.43
156.87

V1 : Isd 37, V2 : Isd 38,
S1 : Row to Row spacing 80cm, S2 : Row to Row spacing 100cm, S3 : Row to Row spacing 120cm,
C0 : Without Sugarcane leaf clipping (NSLC) and C1 : Sugarcane leaf clipping (SLC).

Table 24. Effects of row to row spacing and sugarcane leaf clipping on 1st, 2nd intercrops, equivalent
cane yield of intercrop and total adjusted cane yield.

Treatments Yield of intercrops Equivalent cane yield of Total adjusted cane
V1 S1C0 (t ha-1) intercrop (t ha-1) yield (t ha-1)
V1 S1C1 30.16 114.20
V1 S2C0 1st 2nd 30.68 114.66
V1 S2C1 40.44 134.83
V1 S3C0 6.92 0.19 45.89 139.02
V1 S3C1 6.81 0.23 46.17 137.32
V2 S1C0 9.37 0.24 55.34 147.57
V2 S1C1 9.54 0.43 29.51 109.85
V2 S2C0 10.16 0.36 30.78 112.71
V2 S2C1 10.25 0.74 40.53 127.07
V2 S3C0 6.87 0.17 40.24 126.78
V2 S3C1 6.96 0.21 45.46 127.58
9.52 0.22 53.01 139.44
9.49 0.39
10.28 0.31
10.31 0.63

Price of crops : Sugarcane : 2150.00 Tk t-1 , Potato: 8 Tk kg.-1, Mungbean : 50 Tk kg.-1

Physiology & Sugar Chemistry Division 65

SUGAR CHEMISTRY SECTION shown increasing trend up to February. In case of
SCREENING SUGARCANE CLONES BASED ON reducing sugars content, similar but opposite trends
MATURITY BEHAVIOR were observed from the figure 1(a & b) the clones I
During 2010-11 crushing season, five sugarcane 112-01 and I 137-03 were grouped as early maturing
clones including two standard (Isd 36 & 1sd 37) under potentials. The rest clones were grouped as mid
ZTT-III test stage have been chemically analyzed at a maturing potentials. The same chemical analyses
monthly interval beginning from mid September 2010 have been performed of the eight (08) clones
to mid February 2011. The clones were evaluated on including two standard (Isd 36 & 1sd 37) under ZYT-II
the basis of pol % cane, reducing sugars content and test stage (Fig. 2a, 2b, 2c & 2d). The trend of
fibre % cane and grouped as early, medium and late increasing pol % cane of the clones I 137-03 was
maturing potential clones. Results of chemical higher than the other clones observed peaks in
analysis under ZYT-III test stage have been shown in January and lower trend of reducing sugars content.
Figure 1 (a & b). In case of pol % cane, the trends of The ClonesI 7-03, I 39-04 & I 189-04 under ZYT-II
the clones were observed and found below the may also be treated as early maturing potentials.
standard except one clone (I 137-03) but it were
found quadratic in nature creating peaks in January
(Figure 1a). The clones I 78-03 & I 231-03 were

18.0

2.0

15.0 Isd 36
Isd 37
1.5 I 124-00

Pol % cane Reducing sugar % I 112-01
I 78-03
12.0 Isd 36
Isd 37 1.0 I 137-03
I 124-00 I 231-03
I 112-01
9.0 I 78-03 0.5
I 137-03
6.0 Oct .1 I 231-03 0.0 Oct.10 Nov.10 Dec.10 Jan.11 Feb.11
Sept .1
Nov.1 Dec.1 Jan.1 Feb.11 Sept.10

M o nt hs Mont hs

Figure 1a. Pol % Cane under ZYT-III test stage Figure 1 b. Reducing Sugars % under ZYT-III test stage

16 16

Pol % cane Pol % cane 12

12 Isd 36 8 Isd 36
Isd 37 Isd 37
I 07-00 I 06-04
I 39-04
I 111-03 I 152-04
I 137-03 I 189-04
I 139-03 I 284-04

8 Oct .10 Nov .10 Dec .10 J an.11 Feb.11 4

Sept .10 Sept .10 Oct .10 Nov .10 Dec .10 J an.11 Feb.11

Mont hs M o nt hs

Figure 2a. Pol% Cane under ZYT-II test stage Figure 2b. Pol % Cane under ZYT-II test stage

66 Annual Report, 2010-2011

22

Isd 36

1.5 Isd 36 1.5 Isd 37
Isd 37 I 06-04
Reducing sugar %
Reducing sugar %I 07-00 I 39-04

I 111-03 I 152-04
I 189-04
1 I 137-03 1 I 284-04
I 139-03

0.5 0.5

0 0
Sept.10 Oct.10 Nov.10 Dec.10 Jan.11 Feb.11 Sept.10 Oct.10 Nov.10 Dec.10 Jan.11 Feb.11

Months Months

Figure 2c. Reducing Sugars % under ZYT-II test stage Figure 2d. Reducing Sugars % under ZYT-II test stage

FIBRE CONTENT determined. The results have been shown in bar
The fibre content of sugarcane is directly related with diagram ( Fig. 3a and 3b ). The highest fibre % was
cane quality. Higher is the fibre content lower is the found in the clone I 231-03 (16.75) followed by I 137-
cane quality. It is recognized that the quality of 03 (16.71), Isd 36 (15.86) and I 78-03 (15.85) and the
sugarcane is good when sucrose content is high and lowest fibre content was found in the clone I 124-00
fibre and other impurities are low. The fibre content of (12.36) under ZYT-III test stage. Under ZYT-II test
fiteen (15) sugarcane clones under ZYT-III & II test stage, the highest fibre % cane was observed in the
stages including two standard varieties have been clone I 189-04 (16.05) and the lowest was recorded
from the clone I 139-03(13.25).

20.0
18.0

18.0

16.0 16.0

14.0
14.0

12.0

12.0 10.0
Fibre % Cane 15.86 Fibre % Cane 15.86
14.46 14.46
12.36
13.96 14.76
15.78
15.85
16.71 13.25
16.75 15.54

13.64
15.81
16.05

14.38

Isd 36 Isd 36
Isd 37 Isd 37
I 124-00 I 07-03
I 112-01 I 111-03
I 78-03 I 139-03
I 137-03 I 6-04
I 231-03 I 39-04
I 152-04
I 189-04
I 284-04

Variety/Clone Variety/Clone

Figure 3a. Fibre % Cane under ZYT-III test stage Figure 3b. Fibre % Cane under ZYT-II test stage

Physiology & Sugar Chemistry Division 67

Picture 1. Screening Sugarcane Clones Based on Maturity Behavior

SCREENING SUGARCANE CLONES FOR GUR colour transmittance value was recorded from the
PRODUCTION clones I 284-04 (81.0%) followed by I 124-00 (76.0
Thirteen clones including two standard (Isd 36 & Isd %), I 111-03 (76.0%), I 137-03 (75.0 %), I 7-03 (75.0
37) under ZYT-III and II test stages were evaluated %), I 139-03 (75.0 %), I 112-01 (74.4%), I 189-04
for gur quality. Gur was prepared by open pan boiling (70.0%), I 152-04 (68.5%), I 39-04 (68.0%), I 78-03
method without adding any chemical clarificant. The (67.0%), I 231-03 (67.0%) and I 6-04 (61.0%) ( Figure
physical and chemical properties of gur have been 4e ). On the basis of physical and chemical factors,
studied immediately after its preparation. Gur is gur prepared from the clones I 124-00, I 78-03, I 137-
normally priced in the market for its colour. The higher 03 and I 231-03 under ZYT-III and I 07-03 & I 284-04
colour unit indicates better is the quality. The highest under ZYT-II test stages were found suitable for gur
making.

Table 25. Physical Properties of Gur under ZYT-III & ZYT-II test stages.

Test Stage Variety / Clone Texture Crystalline Nature Colour in Solid State
Standard
ZYT-III Isd 36 Hard Good Golden Yellow
Isd 37 Hard Good Yellow
ZYT-II I 124-00 Hard Good Yellow
I 112-01 Hard Good Golden
I 78-03 Hard Good Yellow
I 137-03 Hard Good
I 231-03 Hard Good Golden Yellow
I 7-03 Hard Good Yellow
I 111-03 Hard Good Yellow
I 139-03 Hard Good Golden
I 6-04 Hard Medium Golden
I 39-04 Hard Medium Golden
I 152-04 Hard Good Golden
I 189-04 Hard Good Golden
I 284-04 Hard Good Golden

Golden Yellow

68 Annual Report, 2010-2011

Reducing Sugars % Gur Sucrose % Gur

12.0 80.0 ZYT-III72.62
71.32
ZYT-III 75.0
70.072.17
9.0 65.068.69

6.0 74.36
71.02
3.0
Variety/Clone Isd 37 6.62 Isd 37 68.20
Physiology & Sugar Chemistry Division I 124-00 8.08 Variety/Clone I 124-00 74.26
Figure 4b. Reducing Sugars% Gur I 112-01 I 112-01 75.79
7.18 Figure 4a. Sucrose % Gur
I 78-03 6.69 I 78-03 69.45
I 137-03 6.70 ZYT-II I 137-03 74.37 ZYT-II
I 231-03 5.73 I 231-03
67.35
I 07-03 5.28 I 07-03 69.48
I 111-03 5.05 I 111-03 71.99
I 139-03 I 139-03
7.12
I 6-04 7.99 I 6-04
I 39-04 I 39-04
I 152-04 4.51 I 152-04
I 189-04 5.41 I 189-04
I 284-04 7.03 I 284-04
7.53

69

70 Annual Report, 2010-2011 pH of Gur Moisture% Gur

Isd 37 7.05.43 16
I 124-00 5.38
I 112-01 ZYT-III5.40 ZYT-III
5.40
I 78-03 6.05.37 12
I 137-03 5.26
I 231-03 5.0 8
5.47
I 07-03 4.0 5.39 4
I 111-03 5.40 Isd 37 10.53
Variety/Clone I 139-03 5.18 Variety/Clone I 124-00 9.17
I 112-01 7.57
Figure 4d. pH of Gur I 6-04 5.54 Figure 4c. Moisture % Gur 5.39
I 39-04 5.47 I 78-03 8.59
I 152-04 5.39 I 137-03 8.76
I 189-04 5.34 I 231-03
I 284-04
ZYT-II I 07-03 7.71 ZYT-II
I 111-03 7.91
I 139-03
9.32
I 6-04 8.07
I 39-04
I 152-04 9.13
I 189-04 6.56
I 284-04 6.26
4.89

100.0 ZYT-III ZYT-II
80.0
Colour Transmittance of gur 76.0 75.0
74.0 76.0
67.0 75.0
75.0 61.0
67.0 68.0
68.5
70.0

81.0

60.0 53.0

40.0

Isd 37
I 124-00
I 112-01

I 78-03
I 137-03
I 231-03

I 07-03
I 111-03
I 139-03

I 6-04
I 39-04
I 152-04
I 189-04
I 284-04

Variety/Clone
Figure 4e. Colour transmittance of Gur

PHOSPHATE CONTENT twenty one sugarcane clones under ZYT-I, II & III test
The phosphate in cane juice is usually present in stages were included for determination of phosphate.
between 250-400 ppm. If phosphate content is low in The highest level of phosphate was found in the
cane juice it is needed to increase phosphate at clones I 6-04 (415 ppm) followed by I 25-04 (393
required level for best form of clarification by adding ppm), I 137-03 (383 ppm), I 231-03 (380 ppm), I 284-
phosphate in slurry or in solution as desired with the 04 (368 ppm), I 78-03 (363 ppm), I 189-04 (358 ppm),
mixed juice. 350 ppm P2O5 content is supposed to be Isd 18 (T2) (358 ppm), I 150-05 (357 ppm), I 112-01
optimum in most of the cane juice in order to get a (350 ppm), I 7-03 (350 ppm), and Isd 18 (T1) (329
good clarification. During 2010-11 crushing season, ppm) ( Fig. 5a, 5b & 5c).

P2O5 (ppm) 600.0 283 P2O5 (ppm) 600.0 283
500.0 243 500.0 350
400.0 400.0
300.0 350 300.0 121
200.0 363 200.0 102
100.0 383 100.0
380 415
0.0 0.0 191

258
358
368

Isd 37 Isd 37
I 124-00 I 07-03
I 112-01 I 111-03
I 139-03
I 78-03
I 137-03 I 6-04
I 231-03 I 39-04
I 152-04
I 189-04
I 284-04

Variety/Clones Variety/Clones

Figure 5a. Phosphate content under ZYT-III test stage Figure 5b. Phosphate content under ZYT-II test stage

Physiology & Sugar Chemistry Division 71

P2O5 (ppm) 500
400
283300
393200
100
185
1790

204
273
357
329
358

Isd 37
I 25-04
I 38-05
I 91-05
I 94-05
I 139-05
I 150-05
Isd 18 (T1)
Isd 18 (T2)

Variety/Clone
Figure 5c. Phosphate content ZYT-I test stage

MANUFACTURE AND PACKAGING OF moisture, colour and pH of granular gur were
GRANULAR GUR found unchanged. The moisture content of
During 2010-11 crushing season, granular gur granular gur was maintained below 2% during
production, packaging and preservation were done. packaging and preservation. The overall physico-
The physical and chemical properties of preserved chemical properties of granular gur were found
granular gur were studied. Results have been shown unchanged after preservation throughout the year.
in bar diagram Fig. 6(a & b). During preservation The preserved granular gur was suitable for
period, sucrose content, reducing sugars content, consumption and attractive in colour.

100 10

Sucrose %, colour80.49 80.42 8 6.71 6.79 5.67 5.64
RS%, Moisture %, pH6
80
Before
storage60

After
storage
Before
storage

After
storage
Before
storage

After
storage
62.0 62.5 4
1.75 1.8

2

0

40

Before After Before After

storage storage storage storage

Sucrose % Colour Reducing Moisture %pH
Sugars % Granular gur

Granular gur

Figure 6a. Sucrose % & Colour transmittance of granular gur Figure 6b. Reducing Sugars%, Moisture% & pH of granular gur

72 Annual Report, 2010-2011

Picture 2. Granular gur packaging and preservation

PREPARATION AND PRESERVATION OF DATE bottled and preserved at room temperature at Sugar
PALM SYRUP Chemistry Laboratory. Brix% Juice, sucrose per cent
In order to determine self-life of date palm syrup, an and reducing sugars per cent of date palm syrup
experiment was conducted at BSRI during 2010-11 were determined before and after preservation. Data
juice tapping season. Juice was collected from date has been presented in the Table 26. It has been
palm garden of BSRI in the months of November, found from data taken that syrup containing 72-78o
December and January. Syrup was prepared and Brix can be preserved easily throughout the year
without deterioration of its quality.

Table 26. Chemical Properties of Date Palm Syrup Before and After Storage.

Parameter 0 Brix Sucrose % Reducing Sugars %
Before Preservation 72-78 55-75 6-8
After Preservation 72-77 55-77 6-9

Picture 3. Date palm syrup preservation Picture 4. Date palm syrup preservation

Physiology & Sugar Chemistry Division 73

PRESENT STATUS OF CANE QUALITY CRUSHED factories suffer due to non-availability of good quality
BY THE SUGAR MILLS AND GUR MAKERS IN cane. An experiment was conducted at NBSM sugar
THE MILLS ZONE mill area during 2010-11 crushing season. Cane was
Sugarcane contains sucrose, reducing sugars, water, collected from gur making places and mills yard at
fibre and other impurities which have great influence NBSM sugar mills in the month of December.
on sugar recovery in the factory. It is universally Collected cane samples were analysed at Physiology
recognized that the quality of sugarcane is good and Sugar Chemistry Laboratory of BSRI. The
when the sucrose content is high and fibre and other samples were analyzed on the basis of non cane%,
impurities are low. It is open truth that recovery is Brix, pol% cane, purity% and reducing sugars
directly related with the cane quality and mills content. Data have been shown in bar graphs 7(a &
performance. During crushing season, huge quantity b). It has been shown from the graph that cane
of sugarcane is diverted for gur processing due to diverted in the mills zone (NBSM) towards gur making
higher gur price in the market specially in the mills was superior in quality than that crushed by the Sugar
zones of NBSM, PBSM, NTSM and RJSM. The sugar Mills for sugar production.

120 6

100 89.32 88.00 5.00
Brix %, Purity % & Pol % cane
5
Gur
Cane80 4

Mill60 3
Cane
Gur 1.98
Cane
2
Mill
Cane
Gur
Cane

Mill
Cane

RS % & % non cane
40 19.5 18.6 13.77 12.91

20 1 0.30 0.47

0 0

Gur Cane Mill Cane Gur Cane Mill Cane

Brix% Purity% Pol% Cane RS% %Non Cane

Figure 7a. Brix%, Purity% and Pol% cane Figure 7b. R.S.%, and Non cane%

EFFECTS OF WATER-LOGGING AND FLOOD time in the month of October, November and
STRESS ON CANE QUALITY December and analyses at Physiology and Sugar
In Bangladesh, one of the most important reasons of Chemistry Laboratory of BSRI. Results of chemical
low cane quality is due to water-logging and flood analysis were shown in line graph. In case of pol %
problem. Estimation shows that about one-third of the cane, the trends of al the varieties were observed
total acreage of sugarcane is planted on land where increasing trend up to December in high land and
water remains stagnant for long period or goes under water-logg stress condition. In case of reducing
flood water resulting in low cane quality. During 2010- sugars content, similar but opposite trends were
11 crushing season, an experiment was conducted to observed. It also observed that the varieties Isd 37
see the cane quality during water-logging period at and Isd 34 were found similar in respect of cane
Lalpur location. Three cane samples were collected quality both in high land and water-logg condition
from water-logged field and high land at the same than the variety Isd 36.

74 Annual Report, 2010-2011

Isd 34 ( HL ) 2 Isd 34 ( HL )
Isd 34 ( WL )
14
1.5
Isd 34 ( WL )

12

Pol % cane10 1
Reducing sugar %

8 0.5

6 Nov.09 Dec.09 0 Nov.09 Dec.09
Oct.09 I sd 3 4 Oct.09 I sd 3 4

Figure 8a. Pol% cane Figure 8b. Reducing sugars

16 1 Isd 36 ( HL )
Isd 36 ( WL )
Isd 36 ( HL )
Isd 36 ( WL ) 0.5

14

12Pol % cane
Reducing sugar %

10

8 Nov.09 Dec.09 0 Nov.09 Dec.09
Oct.09 Isd 36 Oct.09 Isd 36

Figure 9a. Pol% cane Figure 9b. Reducing sugars

Physiology & Sugar Chemistry Division 75

16 1.5

Isd 37 ( HL ) Isd 37 ( HL )
14 Isd 37 ( WL ) 1 Isd 37 ( WL )

12 0.5
Pol % cane
Reducing sugar %10

8

6 Nov.09 Dec.09 0 Nov.09 Dec.09
Oct.09 Isd 37 Oct.09

Isd 37

Figure 10a. Pol% cane Figure 10b. Reducing sugars%

76 Annual Report, 2010-2011

PATHOLOGY DIVISION

RESEARCH HIGHLIGHTS II and I were tested to assess the performance
against pineapple disease (Ceratocystis
Out of 20 commercial varieties 6 were found paradoxa). Among the commercial varieties and
resistant (R), 8 moderately resistant (MR), 2 advanced clones, only a clone I 7-03 was found
moderately susceptible (MS), 1 susceptible (S) as resistant and 2 clones as moderately resistant
and 3 highly susceptible (HS) to red rot. reactions against pineapple disease.
Five clones under ZYT-III were tested of which 1 Eleven genotypes including 6 varieties and 5
clone was found as resistant, 1 moderately promising clones were tested to determine the
resistant and remaining 3 clones were found as incidence of root-knot nematode. In root samples,
susceptible to red rot disease. the highest nematode was found in Isd 36 and
Among 8 clones under ZYT-II, 1 was moderately the lowest Isd 39, I 137-03 and in I 231-03. In soil
resistant, 6 moderately susceptible and 1 was samples, Isd 34 and I 137-03 showed the highest
found as susceptible to red rot. and I 231-03 showed the lowest nematode
Out of 8 clones under ZYT-I, 1 was found as population.
moderately resistant, 3 moderately susceptible An experiment was conducted to evaluate the
and 4 were found as susceptible. effectiveness of some sett treating fungicides
Under AYT out of 28 clones, 1 was found as namely Frescozim 50WP, Erguven 50WP, Nova
resistant, 4 moderately resistant, 6 moderately 50WP and Pedazim 50WP on germination and
susceptible, 11 susceptible and 6 were found as yield of cane. Bavistin 50WP was kept as
highly susceptible. standard. Regarding germination, significant
Among 81 clones under PYT, 39 were found as difference was found between control and other
resistant, 13 moderately resistant, 11 moderately tested fungicides. However, no fungicides
susceptible, 8 susceptible and 10 were highly showed above 80% efficacy over control.
susceptible. To find out the effects of heat therapy on disease
Among 84 tissue culture derived clones, 5 were incidence, growth and yield of cane, an
found as resistant, 4 moderately resistant, 17 experiment was conducted with apparently
moderately susceptible, 15 susceptible and 43 healthy seed materials of 3 varieties viz; Isd 32,
were found highly susceptible. Isd 37 and Isd 38. The treatments were 500C/3
Out of 3 irradiated clones, 1 was found as hrs and 520C/30 min including untreated control.
resistant, 1 moderately resistant and 1 was found No disease was found neither in treated nor in
as moderately susceptible. control treatment in any varieties. Moreover, no
Total 33 genotypes (12 commercial varieties and significance difference was found among the
21 clones under ZYT-I, II and III) were tested treatment of all tested varieties.
against mixed isolate (mixture of four isolates Three sugarcane varieties including one high
from 4 different locations) of Fusarium sacchari sugar content (VMC 86-550) and two (China and
(C.O. of wilt disease). Among the tested Vietnam) having good chewing qualities but
commercial varieties, 9 showed resistant susceptible nature against red rot were exposed
reaction. Under ZYT-III, ZYT-II and ZYT-I 3 8 and in 3 district (Banderban, Rangamati and
7 clones exhibited resistant reaction respectively. Khagrachari) of Chittagong hill tracts to observe
Twenty three sugarcane genotypes comprising their performance in the red soil. Growth
commercial varieties, advanced clones and the conditions of all tested varieties were excellent
clones under ZYT III, II and I were tested against and no major disease (red rot, smut, wilt etc.)
smut (Ustilago scitaminea) disease. The variety were observed. The highest yield was found in
Isd 16 was used as resistant and the variety Isd China by 150.67 t/ha in Banderban. The highest
37 was used as susceptible standard. Among the brix was observed in VMC 86-550 by 18.30% in
tested genotypes, total 8 genotypes (Isd 16, I Khagrachari.
124-00, I 112-01, I 7-03, I 284-04, I 39-04, I 91-05 A total of 1.23 hectare of land was cultivated to
and I 25-04) were found as resistant, 2 were produce foundation seeds of 22 sugarcane
moderately resistant, 5 were moderately varieties. Total 85.4 tons of foundation seeds
susceptible, 4 were susceptible and remaining 3 were produced and per hectare production was
as highly susceptible against smut disease. about 69.5 tons.
Total 23 genotypes including commercial
varieties, advanced clones, clones under ZYT-III,

77

SCREENING SUGARCANE GENOTYPES Only 1 clone was found as moderately resistant, 3
AGAINST RED ROT (Colletotrichum falcatum clones as moderately susceptible and remaining 4
Went.) clones were found as susceptible reaction to red rot
A total of 237 sugarcane genotypes comprising disease (Table-1).
commercial varieties, clones under ZYT-III, ZYT-II, Under AYT, a total of 28 clones were tested of which
ZYT-I, AYT, PYT, tissue culture derived clones and only 1 clone was found as resistant (R). Of the rest
irradiated clones were tested against a mixed isolate clones, 4 were moderately resistant (MR), 6 were
of 17 different isolates of red rot pathogen moderately susceptible (MS), 11 were susceptible (S)
(Colletotrichum falcatum Went) at BSRI farm. Same and remaining 6 were highly susceptible (HS) to red
genotypes were also tested at RSRS, Thakurgaon rot disease (Table-1).
farm against same isolate. Among 20 commercial Eighty one clones under PYT were also tested to red
varieties 6 were found as resistant (R), 8 were rot pathogen of which 39 clones were found as
moderately resistant (MR), 2 were moderately resistant, 13 were moderately resistant, 11 were
susceptible (MS), one variety was susceptible (S) and moderately susceptible, 8 were susceptible and
remaining 3 varieties were found as highly remaining 10 were found highly susceptible.
susceptible (HS) to red rot disease in the field under Eighty four (84) tissue culture derived clones were
prevailing weather condition (Table 1). Under ZYT-III, screened against red rot. Among them, 5 clones were
5 clones were inoculated and among them, one was found as resistant, four were moderately resistant, 17
found as resistant, one was moderately resistant and were moderately susceptible, 15 were susceptible
remaining 3 were found as susceptible to red rot and remaining 43 were found as highly susceptible.
disease. Under ZYT-II, 8 clones were inoculated and Finally, 3 irradiated clones were screened against red
unfortunately no clone was found as resistant. rot. Among them, 1 clone was found as resistant, 1
However, one clone was found as moderately moderately resistant and the rest 1 was found as
resistant, 6 clones were moderately susceptible and moderately susceptible against red rot (Table 1).
the rest one was found as susceptible. Out of 8
clones under ZYT-I, no resistant clone was found.

Table1. Reaction of sugarcane genotypes against Colletotrichu falcatum Went at BSRI farm and RSRS,
Thakurgaon during the cropping season 2009-10.

Disease reaction Name of varieties/clones
(Average of 2 locations) Commercial Varieties (20 varieties)
Isd 2-54, Isd 19, Isd 20, Isd 30, Isd 35 & Isd 39.
R Isd 16, Isd 29, Isd 32, Isd 36, Isd 37, Isd 38, Isd 40 & Co 1158
MR Isd 34 & Co 1148.
MS Isd 33.
S Isd 17, Isd 18 & Isd 28.
HS
R ZYT-III (5 Clones)
MR I 112-01
S I 124-00
MR I 78-03, I 137-03 & I 231-03
MS
S ZYT-II (8 Clones)
MR I 39-04
MS I 6-04, I 152-04, I 189-04, I 284-04, I 7-03 & I 239-03
S I 111-03
R
MR ZYT-I (8 Clones)
MS Isd 18 (T1)
S I 91-05, I 150-05 & I 25-04
HS I 38-05, I 94-05, I 139-05 & Isd 18 (T2)

AYT (28 Clones)
I 226-06
I 140-06, I 170-06, I 204-06 & I 76-05
I 76-06, I 169-06, I 223-06, I 235-06, I 247-06 & I 347-06
I 22-06, I 70-06, I 85-06, I 94-06, I 139-06, I 219-06, I 240-06, I 285-06, I 310-06, I 257-06
& TCBN-05
I 99-06, I 261-06, I 307-06, I 325-06, I 331-06 & I 353-06

78 Annual Report, 2010-2011

Disease reaction Name of varieties/clones
(Result of one location, BSRI PYT (81 Clones)
I 9-07, I 15-07, I 23-07, I 26-07, I 28-07, I 30-07, I 32-07, I 34-07, I 50-07, I 61-07, I 64-07,
farm, Ishurdi) I 67-07, I 68-07, I 74-07, I 92-07, I 98-07, I 99-07, I 103-07, I 110-07, I 129-07, I 131-07,
R I 133-07, I 135-07, I 153-07, I 157-07, I 158-07, I 164-07, I 167-07, I 173-07, I 178-07,
I 195-07, I 200-07, I 215-07, I 219-07, I 220-07, I 224-07, I 235-07, I 238-07& Amrita
MR I 8-07, I 11-07, I 17-07, I 24-07, I 33-07, I 46-07, I 76-07, I 86-07, I 124-07, I 139-07, I 150-
MS 07, I 163-07& I 212-07
S I 2-07, I 48-07, I 65-07, I 71-07, I 77-07, I 82-07, I 123-07, I 201-07, I 208-07, I 213-07&
HS 83R 310
R I 3-07, I 38-07, I 122-07, I 151-07, I 152-07, I 161-07, I 226-07 & VMC 86-550
MR I 10-07, I 52-07, I 54-07, I 89-07, I 115-07, I 162-07, I 180-07, I 190-07, I 210-07 & China
MS Clones derived from tissue culture (84 Clones)
S COK 31(P), LJC (2), LJC (3), 82-1089(4) & 82-1089(5)
HS SL 92-83 (1), K 84-200(9), Co 1158(1) & 82-1089(6)
SL 17-03 (6), SL 92-83 (9), SL 92-83 (9), K 84-200(5), K 84-200(5), VMC 88-354(1), VMC
R 88-354(2), VMC 88-354(1), CP 50-70(3), CP 50-70 (5), COK 31(2), Co 1158 (P), Co
MR 1158(3), NI 8 T1, ISD 16(3),FR 97-131(3) & 82-1089(3)
MS SL 17-03 (8), COK 31(1), ISD 16(P), ISD 16(1), ISD 16(2), ISD 16(4), I52-88(1), FR 97-
131(2), VMC 76-16(1), VMC 76-16(3), 82-1089(1), 82-1089 (2), GP 76-3626 (3), GP 76-
3626 (4) & GP 76-3626(5)
SL 17-03 (3), SL 17-03(7), SL 17-03 (3), SL 17-03 (6), SL 17-03 (7), SL 17-03 (8), K 84-
200(7), K 84-200(7), K 84-200(9), VMC 88-354(2), CP 50-70(P)G3, ISD 18(P), ISD 18(5),
ISD 18(7), ISD 24(1), PR 1000(1), PR 1000(2), I52-88(2), ISD 5-55(1), ISD 5-55(2), FR
97-129(1), FR 97-129(2), FR 97-131(1), VMC 76-16(2), I 68-78(1), I 68-78(2), I 68-78(3),
PHILL 48-15(1), CO 631(1), K 84-200(1), BS 96(1), ISD 17(1), ISD 17(2), ISD 17(3), ISD
17(4), ISD 17(5), ISD 17(6), ISD 17(7), ISD 17 PG(1), ISD 17 PG(2), ISD 17 PG(3), GP
76-3626(1) & GP 76-3626(2)
Irradiated clones (03 Clones)
I 409-06
342 3KR (Isd 32)
I 372-06

R-Resistant, MR-Moderately resistant, MS-Moderately susceptible, S-Susceptible and HS-Highly susceptible.

REACTION OF SUGARCANE GENOTYPES susceptible (MS) against wilt disease. Under ZYT-III,
AGAINST WILT DISEASE (Fusarium sacchari). among 5 tested clones, 4 were found as resistant (R)
Thirty three (33) sugarcane genotypes comprising and another one was moderately susceptible (MS).
commercial varieties, advanced clones, clones under Under ZYT-II, out of 8 clones, all of them were found
Zonal Yield Trial-III, II and I were tested against mixed as resistant (R) against wilt disease. Under ZYT-I,
isolate (mixture of 4 isolates) of F. sacchari wilt at among 8 clones, 7 were found as resistant (R) and
BSRI farm and RSRS Thakurgaon farm. Among 12 remaining one was moderately resistant (MR) against
commercial varieties 9 were found as resistant (R), 2 wilt disease (Table-2).
moderately resistent (MR) and rest one as moderately

Pathology Division 79

Table 2. Reaction of sugarcane genotypes against Fusarium sacchari (causal organism of wilt disease)
at BSRI farm, RSRS Thakurgaon farm and Akandabaria farm of Carew & Co. during the
cropping season 2009-10.

Disease reaction Name of varieties/clones
(Average of 3 locations) Commercial varieties (12 Varieties)
Isd 16, Isd 18, Isd 32, Isd 33, Isd 34, Isd 38, Isd 39, Isd 40 & Co 1148
R Isd 35 & Isd 36
MR Isd 37
MS
R ZYT-III (5 Clones)
MS I 112-01, I 78-03, I 137-03 & I 231-03
R I 124-00
R
MR ZYT-II (8 Clones)
I 6-04, I 39-04, I 152-04, I 189-04, I 284-04, I 7-03, I 111-03 & I 139-03

ZYT-I (8 Clones)
I 150-05, I 139-05, I 94-05, I 91-05, I 25-04, Isd 18 (T1) & Isd 18 (T2)
I 38-05

VARIETAL SCREENING AGAINST SMUT resistant (R), one clone was moderately resistant
Twenty three (23) sugarcane genotypes comprising (MR) and remaining 2 clones were moderately
commercial varieties and the clones under ZYT III, II susceptible (MS). Under ZYT-II, 3 clones were found
and I were tested against smut (Ustilago scitaminea) as resistant (R), 4 clones were susceptible (S) and
disease following spore suspension method. The one was highly susceptible (HS). Under ZYT-I, 2
variety Isd 16 was found as resistant (R) and Isd 37 clones were found as resistant, one moderately
was found as highly susceptible (HS) against smut resistant, 3 moderately susceptible and remaining 2
disease. Under ZYT-III, 2 clones were found as were found as highly susceptible against smut
disease. (Table-3).

Table 3. Reaction of sugarcane genotypes to smut disease (c.o. Ustilago scitaminea) at BSRI farm
during the cropping season 2009-10.

Disease reaction Name of varieties/clones
R Commercial varieties (2 varieties)
HS Isd 16
R Isd 37
MR
MS ZYT-III (5 Clones)
R I 124-00 & I 112-01
S I 137-03
HS I 78-03 & I 231-03
R
MR ZYT-II (8 Clones)
MS I 7-03, I 284-04 & I 39-04
HS I 152-04, I 189-04, I 111-03 & I 139-03
I 6-04

ZYT-I (8 Clones)
I 91-05 & I 25-04
I 94-05
I 139-05, I 150-05 & Isd 18 (T2)
I 38-05 & Isd 18 (T1)

VARIETAL SCREENING AGAINST PINEAPPLE were inoculated by spraying the pathogen
DISEASE Ceratocystis paradoxa over the setts after placement
Total 23 genotypes of sugarcane including in the furrows. Control plots were maintained for each
commercial varieties, clones under ZYT-III, II and I genotype. Germination of sugarcane setts was
were tested to assess the genotypic performance recorded after 60 days of planting. The genotypes
against pineapple disease. Isd 24 and Isd 38 were were assessed considering germination loss in the
taken as susceptible standard. The tested genotypes inoculated plots compared to healthy ones. Both

80 Annual Report, 2010-2011

tested commercial varieties were found as highly was moderately resistant, 2 were moderately
susceptible (HS) against pineapple disease. Under susceptible, 1 was susceptible and the rest 3 were
ZYT-III, out of 5 clones no resistant or moderately highly susceptible against pineapple disease. Under
resistant clones were found. Among the clones 1 was ZYT-I, out of 8 clones, only one clone was found as
found as susceptible and 4 were highly susceptible. moderately resistant. Among the remaining clones, 2
Under ZYT-II, out of 8 clones only one clone was were moderately susceptible, 2 were susceptible and
found as resistant. Among the remaining clones, one 3 were highly susceptible (Table-4).

Table 4. Effect of Pineapple disease (Ceratocystis paradoxa) on germination of different genotypes of
sugarcane (2009-10) (Average of 2 locations).

Sl. No. Name of Genotypes Healthy Germination (%) Difference Remarks
31.92 30.88 (Rating)
1. Isd 24 52.87 Inoculated 26.67
2. Isd 38 40.57 Commercial varieties 31.72 HS
01. I 124-00 60.47 45.31 HS
02. I 112-01 41.92 1.04 30.18 HS
03. I 78-03 44.45 26.20 23.16 HS
04. I 137-03 30.20 ZYT-III (5 clones) 26.25 HS
05 I 231-03 47.24 8.85 28.26 S
01. I 6-04 45.26 15.16 38.41 HS
02. I 39-04 58.59 11.74 18.65 HS
03. I 152-04 52.00 21.30 14.92 HS
04. I 189-04 26.17 13.96 19.72 S
05. I 284-04 8.56 ZYT-II (8 clones) 7.11 MR
06. I 7-03 34.69 18.98 17.87 MS
07. I 111-03 51.43 6.85 36.12 R
08. I 139-03 28.91 39.95 19.10 MS
01. I 38-05 50.83 37.08 28.70 HS
02. I 91-05 43.83 6.46 18.49 MS
03. I 94-05 37.97 1.46 25.70 HS
04. I 139-05 45.55 16.82 24.67 MS
05. I 150-05 50.56 15.31 22.78 HS
06. Isd 18 (T1) 46.67 ZYT-I (8 clones) 27.69 S
07. Isd 18 (T2) 34.14 9.81 14.82 S
08. I 25-04 22.13 HS
25.34 MR
12.27
20.88
27.78
18.98
19.32

* Scale: R ≠ Up to 10% reduction, MR ≠ 10.1 to 15% reduction in germination,
MS ≠ 15.1 to 20% reduction in germination, S ≠ 20.1 to 25% reduction in germination,
HS ≠ above 25% reduction in germination.

THE INCIDENCE OF ROOT-KNOT DISEASE IN 12 months of sugarcane plant by collecting the root
SUGARCANE and soil samples. In root samples, Isd 36 variety
Total 11 genotypes of sugarcane (including 6 showed the highest number of nematode (360/kg
varieties and 5 promising clones) were planted at root). On the other hand, the lowest numbers of
RSRS, Thakurgaon in order to find out the incidence nematode (60/kg root) were found in Isd 39, I 137-03
of root-knot disease in sugarcane. Nematicide and I 231-03. In soil samples, Isd 34 and I 137-03
Furadan 5G was used in the treated plots @ 2kg a.i. genotypes showed the highest number of nematode
ha-1 and check/control plot of each variety were (400/kg soil). On the other hand, the lowest number
maintained. Data were collected on root knot of nematode (40/kg soil) were found in I 231-03 (Fig.
nematode population during harvesting at the age of 1).

Pathology Division 81

450 Control in root Treated in root

400 220 Control in soil Treated in soil 60
Isd 34 80 I 137-03 40
350 360
400 Isd 36 40 400
No. of Nematodes/kg samples 300 40 300 40
80 I 112-01 140
250 100 200
240 60
200 200 80 200 20 200
Isd 37 0 120 200 180
200 160
60
150 0

100 60 100
100
100 Isd 40 0 60 60
40 40
50 I 78-03 20

0 Isd 38 Isd 39 0
0 0 0

I 124-00 I 231-03

Varieties /Clones
Figure 1. Number of nematodes in soil and and root samples in different varieties/clones of sugarcane

From the graph it is very much clear that the RCBD with 3 replications using the variety Isd 32.
nematicide Furadan 5G @ 2 kg a.i. ha-1 significantly Two bud setts of sugarcane were treated by dipping
controlled nematode in the sugarcane field. in 0.1% solution of the fungicides. The duration of
soaking was 30 min. for all fungicides. Sett dipping in
EFFICACY OF SETT TREATING FUNGICIDES ON clean water served as control. Data on germination of
GERMINATION, DISEASE CONTROL AND YIELD setts, millable cane, yield and pol in cane are
OF CANE presented in Table-5.
An experiment was conducted at BSRI farm, Ishurdi Data showed that there is a significant difference
and RSRS, Thakurgaon with a view to evaluate the between control and other tested fungicides.
effectiveness of some sett treating fungicides namely However, among the tested fungicides, no one
Frescozim 50WP, Erguven 50WP, Nova 50WP, showed more than 80% efficacy over control. On the
Pedazim 50WP on germination of setts. Bavistin other hand, regarding yield no significant difference
50WP was used as standard. The trial was laid out was found between control and tested fungicides.

Table 5. Effect of fungicides on germination, disease control and subsequent growth of sugarcane at
RSRS, Thakurgaon farm during 2009-10.

Treatments Germination Yield of cane
Control
Frescozim 50WP Germination (%) % Change over control Yield (t/ha) % Change over control
Erguven 50WP
Nova 50WP 21.19 b - 77.70 a -
Pedazim 50WP 32.32 a 52.52 89.78 a 15.55
Bavistin 50WP 33.22 a 52.05 85.52 a 10.06
32.67 a 54.18 87.6 a 12.74
36.03 a 70.03 92.32 a 18.82
32.67 a 51.00 89.29 a 14.92

*Figures in the column followed by the same letters do not differ significantly as per DMRT test at 5% level of probability.

82 Annual Report, 2010-2011

EFFECTS OF HEAT THERAPY OF SUGARCANE germination. After 45 days of treatment, settlings
SEED MATERIALS ON DISEASE INCIDENCE, were planted in the main field following RCB design.
GROWTH AND YIELD OF CANE Disease incidence was recorded throughout the
To find out the effects of heat therapy on disease season. Number of millable cane, height, girth and
incidence, growth and yield of cane, an experiment yield of cane were recorded at harvest (at the age of
was conducted with three sugarcane varieties viz; Isd 12 months of cane). No disease was found in any
32, Isd 37 and Isd 38 at BSRI farm, Ishurdi. treated or untreated plots. This was perhaps due to
Apparently healthy seed materials of the said use of apparently healthy seed materials. Regarding
sugarcane varieties were treated in hot water at 500C millable cane, Height, girth and yield of cane, no
for 3 hrs. and at 520C for 30 min. Control (without significant difference was found among the
treatment) was maintained for each variety. After treatments in all 3 tested varieties (Table 6).This is
treatment, the seeds were placed in seed bed for also may be due to using apparently healthy seeds.

Table 6. Effects of heat therapy on millable cane, height, girth and yield of cane.

Variety Treatment Millable cane Height Girth (cm) Yield (t/ha)
Isd 32 (000) (m)
Isd 37 T0-Control 2.17 abcd 54.40 abcd
Isd 38 T1-HWT at 500C for 3 hrs 61.77 a 2.56 bcd 2.10 abcd 68.28 a
T2-HWT at 520C for 30 min 66.75 a 2.82 ab 2.09 abcd
T0-Control 60.69 a 2.74 abc 60.75 abc
T1-HWT at 500C for 3 hrs 60.18 a 2.73 abc 2.56 a 53.44 abcd
T2-HWT at 520C for 30 min 58.25 a 2.82 ab 2.44 ab 51.40 abcd
T0-Control 57.18 a 2.97 a 2.45 ab
T1-HWT at 500C for 3 hrs 64.66 a 2.25 de 1.80 cd 63.24 ab
T2-HWT at 520C for 30 min 62.79 a 2.39 cde 2.23 abc 42.16 de
57.35 a 2.17 e 2.01 bcd 49.53 bcd
45.79 cd

PRODUCTION AND QUALITY CONTROL OF against red rot disease were exposed in Chittagong
FOUNDATION SEEDCANE THROUGH CLEAN hill tracts to observe their performance in the red soil
SEED PROGRAMME and hilly areas. Growth and disease incidence of the
A total of 1.23 hectare of land was cultivated to genotypes were monitored throughout the cropping
produce foundation seeds of 22 sugarcane varieties. season. Growth conditions of all the varieties were
Total 85.4 tons of foundation seeds were produced excellent and no major diseases (red rot, wilt, smut
and per hectare production was about 69.5 tons. etc.) were observed in any locations in the hill tracts
The seeds were supplied to Farm Division of BSRI (Banderban, Rangamati and Khagrachari). In case of
and different sugar mills for certified seed production. chewing variety China, the highest yield was found at
Moreover, the produced seeds were supplied to Banderban by 150.67 t/ha and the lowest yield was at
different divisions to set up experiments and Khagrachari by 111.33 t/ha. Incase of another
remaining seeds were supplied to some growers and chewing variety Vietnam, the highest yield was found
sugar mills for crushing. also at Banderban by 130.67 t/ha and the lowest was
PILOTING OF DISEASE RESISTANT HIGH SUGAR at Rangamati by 102.50 t/ha. Regarding the high
CONTENT VARIETIES OF SUGARCANE HAVING sugar content variety VMC 86-550, the highest yield
GUR AND CHEWING QUALITIES IN MADHUPUR of cane was found at Rangamati by 140.00 t/ha and
AND CHITTAGONG HILL TRACTS the brix percent was 18.0. On the other hand the
Three sugarcane varieties including one high sugar lowest yield was at Banderban by 137.00 t/ha with
content (VMC 86-550) and two (China and Vietnam) 17.75 percent brix. In all the locations, considering
having good chewing qualities but susceptible nature chewing quality, both the varieties are excellent. The
variety VMC 86-550 is a high sugar content and self
detrasing in nature which becomes popular for gur
production in the hilly area.

Pathology Division 83

Table 7. Yield, brix and disease incidence of 3 genotypes under Chittagong hill tracts (Average of 5
locations).

Locations Genotypes Millable cane Yield (t/ha) Brix (%) Disease incidence
Bandarban (000)/ha (%)
Rangamati China 47.78 150.67 11.78 0.0
Khagrachari Vietnam 49.38 130.67 14.08 0.0
VMC 86-550 54.11 137.00 17.75 0.0
39.31 115.50 14.10 0.0
China 36.66 102.50 14.65 0.0
Vietnam 48.64 140.00 18.00 0.0
VMC 86-550 40.80 111.33 12.83 0.0
46.25 106.00 14.70 0.0
China 52.97 139.20 18.30 0.0
Vietnam
VMC 86-550

84 Annual Report, 2010-2011

ENTOMOLOGY DIVISION

RESEARCH HIGHLIGHTS RSRS Thakurgaon 111 epiricania adults, 122 top
shoot borer larval parasites and 112 ladybird
In ZYT-I, the lowest (4.93%) top shoot borer beetles were obtained.
infestation was observed in I 25-04 followed by Marigold plant and Tulshi plant as intercrop with
5.84 percent in I 94-04 among the test clones at mechanical control gave 60.47 and 58.76 percent
RSRS, Thakurgaon whereas the highest efficacy respectively in controlling top shoot borer
(24.58%) was in I 139-05 at BSRI, Ishurdi. The whereas only mechanical control showed 51.62
lowest (13.28%) stem borer (stalk basis) percent efficacy against top shoot borer.
infestation was observed in Isd 18(T2) followed by Tobacco dust applied @ 200kg ha-1 in March,
15.28 percent in I 94-05 at RSRS, Thakurgaon. May and July gave 30.62, 30.94 and 31.59
Rootstock borer infestation was very high which percent efficacy against top shoot borer, stem
ranged from 45.54 to 95.10 percent. The lowest borer and rootstock borer respectively.
(7.20%) scale insect infestation was observed in I Detrashing at every 15 days interval showed
150-05 at BSRI, Ishurdi. 35.33 & 30.55 percent efficacy against stem borer
In ZYT-II, among the test clones, the lowest top at Ishurdi and RSRS, Thakurgaon respectively.
shoot borer (8.31%) and stem borer (12.17%) Trichogramma chilonis Ishii @ 50,000ha/week
infestation was observed in I 189-04 and I 152-04 applied in May-September gave 43.56 & 40.65
respectively at RSRS, Thakurgaon. The clone I percent efficacy against stem borer, supplied by
152-04 showed the lowest (38.30%) rootstock Safe Agriculture (Bangladesh) Limited (SABL) and
borer infestation at BSRI, Ishurdi. The lowest Ispahani Biotect (IBT) respectively.
(15.90%) scale insect infestation was observed in M-Furan 5G, Dhanfuran 5G, Bifur 5G, Aungkur
I 139-03 at RSRS, Thakurgaon among the test 5G applied @ 2.0kg a.i ha-1 in March and May
clones and standard varieties. gave over 80 percent control against top shoot
In ZYT-III, no significant difference was observed borer.
in infestation by top shoot borer among the test Some diazinon (10G) insecticides applied @ 1.5 and
clones and standard varieties at both locations 1.75 kg ai.ha-1 in June, July and August failed to
and the infestation ranged from 12.14- 22.05 reach the desired level of efficacy (80%) against
percent. The lowest (4.60% & 5.53%) scale insect stem borer.
infestation was observed in I 137-03 among the Ascend 50 SC, Cipfrin 3GR, Refree 50 SC,
test clones and standard varieties. Refree 3GR and Ruler 3 GR applied @ 50 g ai
The highest infestation of top shoot borer ha-1 at planting gave over 80 percent control
(28.07%), stem borer (19.72%) and rootstock against termite.
borer (33.92%) was observed in May, July and Application of press mud @ 15tha-1 at planting
September at BSRI respectively whereas the gave 27.40 white grubs per 10 clumps and 63.95
highest infestation of top shoot borer (29.62%), percent infestation of rootstock borer whereas
stem borer (37.63%) and rootstock borer untreated (control) plot gave 18 white grubs and
(83.76%) was observed in September, October 64.84 percent rootstock borer infestation.
and November at RSRS Thakurgaon
respectively.
Among the natural enemies 110 epiricania adults,
78 top shoot borer larval parasites and 286
ladybird beetles were observed at BSRI and at

85

REACTION OF DIFFERENT PROMISING ZYT-II (Table 3 & 4): Significant differences were
SUGARCANE CLONES TO MAJOR INSECT observed in infestation by black beetle, top shoot
PESTS borer, stem borer (stalk basis), mealy bug and scale
Screening advanced sugarcane clones against major insect at Ishurdi (Table 3). The standard Isd 33 had
pests is a regular work of this division. For this the lowest infestation which were 13.64%, 49.93% by
purpose different clones planted in the field and top shoot borer and stem borer (stalk basis)
recorded the natural pest incidence for consecutive respectively with the highest (21.41% & 79.30%) in I
three years, which is required by the National Seed 139-03. Higher rootstock borer infestation was
Board (NSB) as pre-requisite to release a variety. observed at Ishurdi which ranged from 38.30-63.00
Under the pest reaction programme three percent among the test clones and standard varieties.
experiments (ZYT-I, ZYT-II & ZYT-III) were carried The lowest (8.71% & 4.74%) mealy bug and scale
out at 2 locations- i. BSRI farm, Ishurdi and ii. RSRS, insect infestation were observed in I 284-04 with the
Madargonj, Thakurgaon during the cropping season highest (16.93% & 21.99%) in standard Isd 37 (Table
2009-2010.The experiments were set up in RCB 3).
design with 3 replications. The plot size was At Thakurgaon, significant differences were observed
maintained 5m x 6m. All the test materials were in early shoot borer, black beetle, top shoot borer,
subjected to natural infestation by Early shoot borer stem borer (stalk basis), mealy bug and scale insect
(ESB), Top shoot borer (TSB), Stem borer (SB), infestation (Table 4) among the test clones and
Rootstock borer (RSB), Scale insect (SI), Mealy bug standard varieties. Early shoot borer and black beetle
(MB), White grub (WG), Black beetle (BB) etc. infestation was low among the test clones and
ZYT-I (Table 1 & 2): Significant differences were standard varieties. The highest (26.84% & 25.27%)
observed among the test clones and standard top shoot borer and stem borer infestation (stalk
varieties for early shoot borer, black beetle, top shoot basis) were observed in I 39-04. Heavy rootstock
borer, stem borer (stalk basis), rootstock borer and borer infestation was observed at Thakurgaon which
scale insect infestation at Ishurdi (Table 1). The ranged 80.94-93.03 percent among the test clones
lowest (0.19% & 2.80%) early shoot borer and black and standard varieties. In case of mealy bug and
beetle infestation were found in Isd 18(T2) with the scale insect the clone I 139-03 had the lowest
highest (2.29% & 9.48%) in I 139-05. The lowest (23.90% & 15.90%) infestation with the highest
(9.97% & 38.40%) top shoot borer and stem borer (73.78% & 61.87%) infestation in standard Isd 37
infestation (stalk basis) were observed in standard Isd (Table 4).
33 with the highest (24.58% & 60.02%) in I 139-05. ZYT-III (Table 5 & 6): Among the test clones and
Rootstock borer infestation was very high which standard varieties, no statistically significant
ranged from 45.54-77.31 percent. Mealy bug difference were observed in infestation by top shoot
infestation varied from 15.31-28.29 percent among borer, stem borer (stalk basis), stem borer (internode
the test clones/varieties. Scale insect infestation basis), mealy bug and scale insect at Ishurdi (Table
ranged from 7.20-35.73 percent (Table 1). 5). At Thakurgaon, test clones showed statistically
significant difference in infestation by black beetle,
At RSRS, Thakurgaon, all the test clones and stem borer (stalk basis), stem borer (internode basis),
standard varieties showed statistically significant mealy bug and scale insect (Table 6). Early shoot
differences for the target pests (Table 2). Early shoot borer infestation was low at both locations. In case of
borer infestation was very low among the test clones top shoot borer, the lowest (14.93% & 12.14%)
and standard varieties. The clone I 139-05 had the infestation was observed in standard Isd 36 (Table 5
highest infestation which was 1.90%, 4.73%, 18.71% & Table 6). Higher stem borer (stalk basis) infestation
and 29.42% by early shoot borer, black beetle, top was noticed at Ishurdi which varied from 60.55-73.98
shoot borer and stem borer (stalk basis) respectively. percent and 19.51-32.98 percent at Thakurgaon
The lowest (3.84%) top shoot borer infestation was among the test clones and standard varieties. Heavy
observed in standard Isd 33. Rootstock borer rootstock borer infestation was observed at
infestation was very high which ranged from 65.70- Thakurgaon which ranged from 71.94-90.49 percent
95.10 percent. Standard variety Isd 33 showed the and 41.54 - 62.75 percent at Ishurdi among the test
lowest (26.10% & 25.38%) mealy bug and scale clones and standard varieties. The lowest (13.46%)
insect infestation and the clone I 38-05 had the mealy bug infestation was observed in I 231-03 and
highest (46.22% & 59.64%) infestation in the same the highest (18.81%) in I 78-03 at Ishurdi whereas at
pests (Table 2).

86 Annual Report, 2010-2011

Thakurgaon, the lowest (21.42%) infestation was scale insect the clone I 137-03 showed the lowest
observed in I 7-03 and the standard variety Isd 37 (4.60% and 5.53%) infestation with the highest
showed the highest (74.34%) infestation. In case of (54.94%) in standard Isd 37(Table 5 & 6).

Table 1. Infestation levels of some major insect pests to promising BSRI clones (ZYT-I). BSRI, Ishurdi,
Pabna. 2009-2010.

Clones/Variety Percent infestation (Mean)
I 25-04
I 38-05 ESB BB TSB SB SB RSB MB SI
I 91-05 1.01abcd 5.57ab 14.87b (stalk) (internode) 56.00ab 24.35 10.22ab
I 94-05 7.19ab 14.02b 61.09ab 21.75 19.23ab
I 139-05 2.25a 7.77ab 13.47bc 48.00abcd 35.42 64.20ab 17.41 13.58ab
I 150-05 1.15abcd 9.36a 15.45b 59.48ab 33.63 57.74ab 15.31 20.66ab
Isd 18 (T1) 9.48a 24.58a 53.88abc 23.46 77.31a 20.23 11.10b
Isd 18 (T2) 1.99ab 9.44a 12.46bc 40.77cd 26.28 45.54b 24.02
Isd 33 2.29a 5.01ab 12.50bc 60.02a 25.87 70.11ab 20.91 7.20b
Isd 37 1.73abc 2.80b 14.62b 45.59abcd 23.12 71.81a 21.71 17.12ab
LSD (5%) 0.58cd 5.90ab 9.97c 55.34abc 27.14 65.67ab 15.42 35.73ab
0.19d 5.39ab 14.42b 44.88bcd 28.91 60.52ab 28.29 32.01ab
0.83bcd 4.602 3.739 38.40d 32.76 32.06a
1.38abcd 47.14abcd 30.68 21.60 NS
1.212 13.13 NS 17.46

ESB = Early shoot borer, BB = Black beetle, TSB = Top shoot borer, SB = Stem borer, RSB = Rootstock borer
MB = Mealybug, SI = Scale insect
* Figures followed by the same letter (s) are not significantly different at 5% level as per LSD test

Table 2. Infestation levels of some major insect pests to promising BSRI clones (ZYT-I). RSRS,
Thakurgaon. 2009-2010.

Clones/ Percent infestation (Mean)
Variety
I 25-04 ESB BB TSB SB SB RSB MB SI
I 38-05 0.54bc 1.36b 4.93e (stalk) (internode) 95.10a 38.89ab 32.55b
I 91-05 1.59ab 3.85a 6.91cde 20.19abc 65.70c 46.22a 59.64a
I 94-05 0.74abc 0.83b 9.76bcde 25.95ab 22.69a 89.12ab 37.66ab 42.26ab
I 139-05 0.78abc 1.23b 5.84de 25.51ab 21.81ab 89.78ab 29.70ab 35.29ab
I 150-05 1.90a 4.73a 18.71a 15.28bc 22.14a 94.20a 26.93b 42.43ab
Isd (T1) 0.19c 0.78b 13.56ab 29.42a 13.76ab 70.14bc 37.13ab 26.81b
Isd 18(T2) 0.49bc 1.33b 12.51bc 18.40bc 21.33ab 92.24ab 40.13ab 33.24b
Isd 33 1.03abc 1.80b 11.59bcd 22.33abc 13.00b 92.69a 30.60ab 58.66a
Isd 37 0.75abc 1.83b 4.15e 13.28c 15.55ab 86.94ab 26.10b 25.38b
LSD (5%) 0.59bc 1.11b 3.84bcde 23.54abc 18.38ab 90.23ab 31.53ab 43.14ab
1.284 2.493 5.529 20.18abc 15.81ab 20.00 21.31
9.680 19.70ab 14.73

7.948

ESB = Early shoot borer, BB = Black beetle, TSB = Top shoot borer, SB = Stem borer, RSB = Rootstock borer
MB = Mealybug, SI = Scale insect
* Figures followed by the same letter (s) are not significantly different at 5% level as per LSD test

Entomology Division 87

Table 3. Infestation levels of some major insect pests to promising BSRI clones (ZYT-II). BSRI, Ishurdi,
Pabna. 2009-2010.

Clones/ Percent infestation (Mean)
Variety
I 139-03 ESB BB TSB SB SB RSB MB SI
I 6-04 0.99ab 1.73ab 11.13bc (stalk) (internode) 93.03 23.90c 15.90c
I 39-04 0.37b 1.19bc 12.99bc 81.78 73.70a 28.33bc
I 152-04 1.20ab 0.39c 26.84a 15.50ab 26.34 81.95 44.65bc 29.21bc
I 189-04 1.34ab 1.15bc 14.03b 18.36ab 23.24 87.00 55.68ab 59.07a
I 284-04 2.03a 1.79ab 8.31cd 25.27a 17.96 92.67 34.34bc 48.39ab
Isd 33 2.01a 2.68a 16.56b 12.17b 22.42 80.94 38.02bc 24.82c
Isd 37 0.81ab 0.61c 19.70ab 19.60 91.00 37.16bc 33.23bc
LSD (5%) 0.60ab 2.22ab 4.25d 23.59a 27.37 81.80 73.78a 61.87a
1.320 1.030 11.79bc 20.02ab 22.27 25.84 20.41
25.07a 21.19 NS
5.056 9.491 NS

ESB= Early shoot borer, BB= Black beetle, TSB= Top shoot borer, SB= Stem borer, RSB= Rootstock borer
MB = Mealybug, SI= Scale insect
* Figures followed by the same letter (s) are not significantly different at 5% level as per LSD test

Table 4. Infestation levels of some major insect pests to promising BSRI clones (ZYT-II). RSRS,
Thakurgaon. 2009-2010.

Clones/ Percent infestation (Mean)
Variety
I 139-03 ESB BB TSB SB SB RSB MB SI
I 6-04 1.19 4.07ab 21.41a (stalk) (internode) 63.00 10.26bc 6.43b
I 39-04 0.90 3.84ab 15.20ab 42.10 14.52ab 6.54b
I 152-04 0.89 3.64ab 18.01ab 79.30a 44.33 55.01 12.93abc 10.42b
I 189-04 0.60 1.87b 17.59ab 53.48c 32.28 38.30 13.27abc 12.04b
I 284-04 0.76 2.45ab 17.12ab 69.52ab 36.06 49.92 11.00bc 11.43b
Isd 33 0.56 2.97ab 15.18ab 54.29bc 35.77 46.42 4.74b
Isd 37 1.41 5.08a 13.64b 62.85bc 36.52 56.89 8.71c 9.90b
LSD (5%) 0.89 4.61ab 17.00ab 63.42bc 44.04 50.24 12.18abc 21.99a
NS 2.493 5.961 49.93c 47.48 8.935
59.95bc 33.57 NS 16.93a
14.38 NS 4.667

ESB= Early shoot borer, BB= Black beetle, TSB= Top shoot borer, SB= Stem borer, RSB= Rootstock borer
MB = Mealybug, SI= Scale insect
* Figures followed by the same letter (s) are not significantly different at 5% level as per LSD test

Table 5. Infestation levels of some major insect pests to promising BSRI clones (ZYT III). BSRI,Ishurdi,
Pabna. 2009-2010.

Clones/Varieties Percent infestation (mean)

I 7-03 ESB BB TSB SB SB RSB MB SI
I 78-03 (stalk) (internode)
I 111-03 0.23b 2.02b 17.29 41.54b 15.52 10.17
I 137-03 1.33a 3.56a 22.05 60.55 26.26 62.75a 18.81 14.74
I 231-03 0.33b 2.05b 18.41 73.98 24.29 51.59ab 16.74 8.43
Isd 36 0.00b 0.70b 16.30 66.29 26.48 49.11ab 16.46 4.60
Isd 37 0.71ab 2.17b 15.09 60.89 39.37 47.63ab 13.46 9.13
LSD (5%) 0.53a 4.64a 14.93 71.10 27.43 50.51ab 14.82 10.05
0.73ab 4.10a 15.59 51.36ab 15.11 9.69
0.695 1.372 68.46 30.49 14.09
NS 65.79 35.49 NS NS
NS NS

ESB= Early shoot borer, BB= Black beetle, TSB= Top shoot borer, SB= Stem borer, RSB= Rootstock borer
MB = Mealybug, SI= Scale insect
* Figures followed by the same letter (s) are not significantly different at 5% level as per LSD test

88 Annual Report, 2010-2011

Table 6. Infestation levels of some major insect pests to promising BSRI clones. (ZYT III). RSRS,
Thakurgaon, 2009-2010.

Clones/ Percent infestation (mean)
Varieties
ESB BB TSB SB SB RSB MB SI
I 7-03 (stalk) (internode)
I 78-03 2.05 1.70b 16.76 79.23 21.42b 13.59b
I 111-03 1.32 3.15ab 12.16 29.87ab 10.18b 87.52 26.51b 51.31a
I 137-03 1.90 2.27ab 20.67 21.15ab 18.11a 85.74 37.43b 43.84a
I 231-03 0.88 2.02ab 18.72 31.94ab 14.25ab 90.49 64.66a 5.53b
Isd 36 0.74 1.52b 13.10 32.98a 21.42a 71.94 38.32b 47.77a
Isd 37 1.35 1.98ab 12.14 26.08ab 18.88a 88.55 71.85a 16.12b
LSD (5%) 1.59 3.49a 18.48 19.51b 18.83a 88.78 74.34a 54.94a
NS 1.619 22.71ab 14.24ab 20.44 19.10
NS 11.69 6.695 NS

ESB= Early shoot borer, BB= Black beetle, TSB= Top shoot borer, SB= Stem borer, RSB= Rootstock borer
MB = Mealybug, SI= Scale insect
* Figures followed by the same letter (s) are not significantly different at 5% level as per LSD test

STUDIES ON THE SEASONAL ABUNDANCE OF found more than 10. Highest (71) number of pyrilla
MAJOR INSECT PESTS OF SUGARCANE AND nymph was found in September’09. Total number of
THEIR NATURAL ENEMIES natural enemies of major sugarcane insect pests was
The experiment was set up at two locations, one at recorded from February’09 to January’10. Among
BSRI farm and another at RSRS farm, Thakurgaon them 110 epiricania adult, 78 top shoot borer larval
during the cropping season 2009-2010. Variety Isd 34 parasite and 286 ladybird beetle were recorded
was used as planting material at Ishurdi and Isd 32 at (Table 8).
RSRS, Thakurgaon. Results revealed that early shoot At RSRS, early shoot borer infestation was found
borer infestation was observed from the month of very low. Top shoot borer infestation was increased
February’09 to April’09 and infestation ranged from from March’09 to September’09 (15.05-29.62%).
0.64-6.21percent. Highest (28.07%) top shoot borer Rootstock borer infestation started from April’09 and
infestation was observed in May’09 followed by 23.34 continued up to January’10 that ranged from 18.27-
percent in April’09. More than 20 percent rootstock 83.76 percent. In case of stem borer the incidence
borer infestation was observed from April’09 to was increased from June’09 to October’09 (18.10-
December’09. Stem borer infestation started from 37.63%) and the infestation started decrease from
June’09 and the infestation was above 11 percent up November’09 to January’10 (17.01-10.22%). From
to December’09. Minimum (10.46%) and maximum February’09-May’09 more than 10 percent black
(49.66%) black beetle infestation was found in May’ beetle infestation was observed (Table 9).
09 and March’09 respectively (Table 7). Maximum In case of adult, total number of top shoot borer,
leaf cutting weevil was recorded from June to rootstock borer, stem borer, black beetle, pyrilla leaf
September at both locations (Table 8 & 10). hopper, pyrilla nymph and leaf cutting weevil
In case of adult, highest number of top shoot borer recorded were 120, 73, 64, 51, 378, 161 and 78
and pyrilla leaf hopper recorded were 22 and 662 respectively (Table 10). One hundred eleven (111)
respectively in June’09. From May’09 to epiricania adult, 122 top shoot borer larval parasites
September’09 the number of stem borer adult was and 112 lady bird beetles were recorded (Table 10).

Entomology Division 89

Table 7. Month wise percent infestation of major insect pests of sugarcane, BSRI farm, Ishurdi, Pabna,
2009-2010.

Month Percent infestation of major insect pests
February’ 09
March, 09 ESB TSB RSB SB BB
April’ 09 5.10 43.00
May’09 6.21 0 0 0 49.66
June’ 09 0.64 12.17 13.04 0 19.37
July , 09 23.34 26.60 0 10.46
August’09 0 28.07 26.95 0
September’09 0 19.11 25.71 12.86 0
October’09 0 11.42 26.29 19.72 0
November’ 09 0 11.09 27.73 17.44 0
December’ 09 0 8.11 33.92 15.30 0
January’ 10 0 7.58 30.57 15.38 0
0 4.29 23.92 13.73 0
0 1.37 22.86 11.13 0
0 0.09 2.49 1.02 0

Table 8. Month wise no. of moth/adult/beetle & natural enemies of major sugarcane insect pests (per 10
sweeping), BSRI farm, Ishurdi, Pabna, 2009-2010.

No. of moth/adult/beetle & natural enemies of major sugarcane insect pests (per 10 sweeping)

Month TSB moth RSB SB Moth BB Pyrilla Pyrilla Leaf Epiricania TSB Lady bird
Moth leaf nymph cutting adult larval beetle
weevil parasite
hopper

February’ 09 3 0 0 18 0 0 0 0 0 7
March,09 6 0 0 19 18 14 0 0 13 10
April’ 09 9 0 9 15 89 19 0 9 14 23
May’09 17 6 11 10 132 18 0 17 11 77
June’ 09 22 5 12 0 662 17 19 13 13 76
July , 09 15 4 14 0 111 19 16 19 12 44
August’09 11 5 18 0 146 59 23 11 11 14
September’09 0 0 13 0 242 71 19 12 4 18
October’09 0 0 0 0 80 45 9 16 0 10
November’ 09 0 0 0 0 27 39 8 13 0 7
December’ 09 0 0 00 0 0 0 0 0 0
January’ 10 0 0 00 0 0 0 0 0 0
Total 81 20 67 62 1507 301 94 110 78 286

Table 9. Month wise percent infestation of major insect pests of sugarcane, RSRS, Thakurgaon,
2009-2010.

Month Percent infestation of major insect pests
February’ 09
March,09 ESB TSB RSB SB BB
April’ 09 1.71 10.04
May’09 1.52 4.22 0 0 11.13
June’ 09 15.05 0 0 12.05
July ,09 0 18.07 22.24 0 11.99
August’09 0 18.12 23.96 8
September’09 0 23.15 18.27 18.10 0
October’09 0 24.72 57.26 26.84 0
November’ 09 0 26.50 67.85 27.68 0
December’ 09 0 29.62 79.48 29.13 0
January’ 10 0 13.10 82.91 37.63 0
0 11.37 83.76 17.01 0
0 10.33 47.35 16.27 0
0 7.23 19.02 10.22 0

90 Annual Report, 2010-2011

Table 10. Month wise no. of moth/adult/beetle & natural enemies of major sugarcane insect pests (per 10
sweeping), RSRS, Thakurgaon, 2009-2010.

No. of moth/adult/beetle & natural enemies of major sugarcane insect pests (per 10 sweeping)

Month TSB RSB SB Moth BB Pyrilla Pyrilla Leaf Epiricani TSB Lady
February’ 09 moth moth leaf nymph cutting a adult larval bird
March,09 weevil parasite beetle
April’ 09 hopper
May’09
June’ 09 9 3 0 15 0 0 0 0 19 11
July ,09 12 12 0 15 0 0 0 0 11 17
August’09 13 15 13 11 11 14 4 9 12 16
September’09 16 14 13 10 24 21 6 17 23 18
October’09 15 11 11 0 125 54 23 23 14 14
November’ 09 14 9 14 0 115 24 16 19 23 11
December’ 09 11 2 13 0 65 14 17 11 8 13
January,10 9 0 0 0 15 9 11 12 7 9
Total 8 0 0 0 10 10 1 16 5 3
8 3 0 0 13 15 0 13 0 0
5200000000
0200000000
120 73 64 51 378 161 78 111 122 112

USE OF MARIGOLD AND TULSHI PLANT IN were seven (7) treatments including one control.
CONTROLLING SUGARCANE TOP SHOOT Plantation was done with the variety Isd 32. Results
BORER revealed that (pooled data) all the treatments showed
The experiment was set up at BSRI farm, Ishurdi, more than 50 percent efficacy among the test
Pabna during the cropping season 2009-2010.The treatments over control. Marigold plant as intercrop
experiment was laid out in Randomized Complete with mechanical control gave the highest efficacy of
Block Design (RCBD) with three replications.There 60.47 percent in controlling top shoot borer (Table
11).

Table 11. Effects of Marigold and Tulsfi plant for the control of sugarcane top shoot borer. BSRI farm
Ishurdi, Pabna. 2009-2010.

Percent top shoot borer infestation (Mean) Pooled data
(percent
Treatments 2nd data 3rd data 4th data Final data efficacy)
01.04.10 02.05.10 02.06.10 59.90
50.45
T1 : Marigold plant as intercrop 2.49b 2.77c 6.34cd 9.79b 60.08
(5.37) (64.80) (61.71) (47.73) 51.62
T2 : Marigold leaves extracts @ 25 3.11b 3.92b 8.72b 9.83b 60.47
kg ha-1 (March, April & May) (6.74) (50.19) (47.34) (47.52) 58.76
T3 : Tulshi plant as intercrop 2.54b 3.03c 6.42cd 8.82b -
(4.67) (61.50) (61.23) (52.91)
T4 : Mechanical control (March+May) 3.54b 2.99c 8.16bc 10.68b
(50.76) (62.01) (50.72) (42.98)
T5 : Marigold plant + Mechanical 2.52b 3.02c 5.88d 9.21b
control (March + May) (64.95) (61.63) (64.49) (50.83)
2.81b 3.05c 6.52cd 8.94b
T6 : Tulshi plant as intercrop+ (60.92) (61.24) (60.63) (52.27)
Mechanical control (March+May) 7.19a 7.87a 16.56a 18.73a
1.212 0.6111 2.008 1.699
T0 : Control
LSD (5%)

* Figures followed by the same letter (s) are not significantly different at 5% level as per LSD test
Figures in parenthesis are percent effectiveness over control.

Entomology Division 91

USE OF TOBACCO DUST IN CONTROLLING borer and 10.27-30.57 percent efficacy against
SOME MAJOR PESTS OF SUGARCANE rootstock borer infestation. The highest (61.00tha-1)
An experiment was conducted at BSRI farm, Ishurdi, yield was recorded in tobacco dust @ 200kgha-1
Pabna and RSRS, Thakurgaon during the cropping treated plot when applied in March + May + July at
season 2009-2010 with the variety Isd 34 and Isd 32 Ishurdi (Table 12).
at Ishurdi and Thakurgaon respectively. The At RSRS, Thakurgaon, significant difference was
experiment was laid out in Randomized Complete observed against rootstock borer infestation among
Block Design (RCBD) with three replications. There the treatments over control (Table 13). Data showed
were seven (7) treatments in the experiment including that tobacco dust applied @ 100-200kg ha-1 gave
one control. No significant differences were observed 19.86-33.62 percent efficacy against top shoot borer,
among the treatments for top shoot borer, stem borer 3.61-30.94 percent efficacy against stem borer and
and rootstock borer infestation at Ishurdi (Table 12). 2.58-31.59 percent efficacy against rootstock borer
Data showed that tobacco dust applied @ 100-200kg infestation.The highest (66.78tha-1) yield was
ha-1 gave 6.24-25.94 percent efficacy against top recorded in tobacco dust @ 200kgha-1 treated plot
shoot borer, 2.87-18.40 percent efficacy against stem when applied in March + May + July (Table 13).

Table 12. Effects of tobacco dust in controlling some major insect pests of sugarcane, BSRI, Ishurdi,
Pabna. 2009-2010.

Treatments TSB Percent Infestation RSB Yield
T1 : Tobacco dust @ 100kg ha-1 (P+M+M+July) 20.45 SB 56.62
T2 : Tobacco dust @ 100kg ha-1 (M+M+July) (8.21) (10.27) (tha-1)
T3 : Tobacco dust @ 150kg ha-1 (P+M+M+July) 20.89 47.77 53.36
T4 : Tobacco dust @ 150kg ha-1 (M+M+July) (6.24) (2.87) (15.44) 49.00cd
T5 : Tobacco dust @ 200kg ha-1 (P+M+M+July) 19.57 47.25 49.72 (0.68)
T6 : Tobacco dust @ 200kg ha-1 (M+M+July) (12.16) (3.93) (21.20) 50.11cd
18.23 47.15 46.40 (2.96)
T0 : Control (Untreated) (18.18) (4.13) (26.47) 52.89bc
16.50 45.97 46.40 (8.67)
LSD (5%) (25.94) (6.53) (26.47) 57.67bc
17.20 41.20 43.81 (18.49)
(22.80) (16.23) (30.57) 56.22b
22.28 40.13 63.10 (15.51)
(18.40) 61.00a
(-) 49.18 (-) (25.33)
NS NS 48.67d
(-)
NS (-)
3.636

* Figures followed by the same letter (s) are not significantly different at 5% level as per LSD test
Figures in parenthesis are percent effectiveness over control.

92 Annual Report, 2010-2011