5 Steps to accelerate your research

5 steps to accelerate your research

Version 6 | January 2019

Index 3 Fueling your inspiration

Our life sciences portfolio 4
Life science research in a cell
The Pluripotent Stem Cell Workflow Understanding the full extent of the research resources available can
Cell Engineering Workflow
Gene-Modified T Cell Manufacturing Workflow 5 sometimes prove a difficult prospect, diverting crucial time away from your
Gene Expression Workflow
Endpoint PCR Workflow research and frustrating even the most experienced of scientists among us.
DNA Variant Detection Workflow
Molecular Cloning Workflow 6 This guide is designed to help you easily discover the many ways that we can
Protein Expression Workflow
Protein Analysis Application 7 help you to achieve your research goals by clearly presenting our five-step
Cell Analysis Application workflow solutions in one place.
Useful Resources
8 Use the index to navigate to your research area to learn more and if you have
any questions, our full collection of useful resources can be found on page 15.

9 Don’t let searching for the solution get in the way of your inspiration.

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Our life sciences portfolio

Thermo Scientific™, Applied Biosystems™, Invitrogen™, Gibco™ and Ion Torrent™ are Thermo Fisher Scientific brands within the life sciences product portfolio.
Molecular Probes™, Novex™, eBioscience™ and Ambion™ are product line names under the Invitrogen brand.
Here’s an overview of how our life sciences brands and product lines come together:

Bioproduction, molecular biology, protein Integrated systems for sequencing, real- Cell, protein and molecular biology Media, supplements, cells and reagents. Sequencing technology powered by
biology, high-content analysis and time, digital and endpoint PCR. technologies. semiconductor chips.
laboratory essentials. Popular product lines
Popular product lines Popular product lines Popular product lines GlutaMAX™ Popular product lines
Phusion™ QuantStudio™ Molecular Probes™ B-27™ Plus Ion AmpliSeq™
FastDigest™ SeqStudio™ GeneArt™ TrypLE™ Ion Chef™
GeneRuler™ GeneStudio™ TOPO™ KnockOut™ Ion PGM™
SuperSignal™ MycoSEQ™ Countess™ Neurobasal™ Ion GeneStudio™ S5 Series
Slide-A-Lyzer™ MagMAX™ SuperScript™ Advanced™ Ion Proton™
HyPerforma™ ExoSAP-IT™ Lipofectamine™ FluorBrite™ Ion Reporter™
Tandem Mass Tag™ BigDye™ Dynabeads™ Stemflex™ Oncomine™
CellInsight™ TaqMan® EVOS™ DMEN Oncomine Knowledgebase Reporter™
Zeba™ TaqPath™ Attune™ Geltrex™ Ion Xpress™
KingFisher™ SYBR™ eBioscience™ CTS™ Ion TargetSeq™
DreamTaq™ BigDye™ ProcartaPlex™ ExpiCHO™
Maxima™ ProFlex™ QuantiGene™ Expi293™
Nunc™ SimpliAmp™ ProQuantum™ ExpiSf™
Nalgene™ Veriti™ Novex™
Multiskan™ MicroAmp™ Qubit™
Varioskan™ Alexa FLuor Plus™
Nunclon™ iBind™
MultiDrop™ iBright™
ClipTip™ TrueGuide™
Owl™ TrueCut™
Finnpipette™ PrimeFlow™
E-Gel™
Purelink™
Anza™
Alexa Fluor™
Platinum™

Where can you find all of these products? Visit: www.thermofisher.com

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Life science research in a cell

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The Pluripotent Stem Cell Workflow

Unrivalled solutions for generating and exploring disease models

Culture Reprogramme Expand Engineer Differentiate

Cell culture media and supplements Reprogramme adult cells such as iPSC expansion requires careful Engineer your induced pluripotent Once cells have been
are required to grow the cells before blood cells back to a pluripotent attention to culture conditions stem cells to analyse gene and reprogrammed they are then
reprogramming state to become iPSCs protein expression and study differentiated into specific cell types
differentiation and disease pathways

Human Somatic Cells Viral Culture Media • Invitrogen™ GeneArt™ Precision TALs Gibco™ products:
• Blood (CD34+) • Invitrogen™ Trueguide™ and • Essential 6™ Medium (reprogramming
• Fibroblast •• Gibco™ CytoTune™ iPS Sendai H• Gibco™ StemFlex™ Medium
• Keratinocyte Reprogramming Kit HTrueCut™ CRISPR editing tools and differentiation)
• Epithelial (corneal, mammary) • Applied Biosystems™ TaqMan® •• Gibco™ Essential 8™ Medium
•• Gibco™ Essential 8™ Flex Medium Transfection H• CTS™ Essential 8™ Medium
Culture Media iPSC Sendai Detection Kit • Invitrogen™ Lipofectamine™
• Gibco™ Advanced™ DMEM H• Gibco™ CTS™ Essential 8 • PSC Neural Induction Medium
• Gibco™ DMEM/F12 DNA Medium HStem • PSC Dopaminergic Neuron
• Invitrogen™ Episomal Vectors • Gibco™ KnockOut™ Serum
H• Gibco™ One Shot™ FBS • Invitrogen™ Lipofectamine 3000 ™ • Invitrogen™ Lipofectamine™ HDifferentiation Kit
Replacement (mouse) CRISPRMAX™ H• CultureOne™ Supplement (neural)
Supplements Reagent H• B-27™ Plus Supplement (neural)
• Balanced salt solutions (PBS) Supplements • Invitrogen™ Neon™ Transfection
• Growth factors thermofisher.com/cellreprogram • Gibco™ RevitaCell™ Supplement System • PSC Cardiomyocyte Differentiation
• Recombinant proteins • Gibco™ PSC Medium
• Thermo Scientific™ Nunclon™ Laboratory Essentials: Genetic Analysis
• Biological Safety Cabinets Cryopreservation Kit • Applied Biosystems™ • PSC Definitive Endoderm Induction
Delta flasks, plates and dishes • Manual Pipettes
• Nunclon™ Delta flasks, plates Matrices QuantStudio™ Real-Time PCR HMedium Kit
• Gibco™ Geltrex™ hESC- Systems and Reagents
and dishes • Ion™ GeneStudio™ S5 Series Next- thermofisher.com/differentiation
qualified Ready-To-Use matrix Generation Sequencing Systems and
• Gibco™ Vitronectin Human Protein, Kits Laboratory Essentials:
• Applied Biosystems™ SeqStudio™ • Standard Cryotubes/2D Tubes
Natural Genetic Analyzer and Kits
• CO2 Incubators
thermofisher.com/ thermofisher.com/genomeedit • Biological Safety Cabinets
stemcellculture
Laboratory Essentials: • Benchtop Centrifuges
Laboratory Essentials: • Biological Safety Cabinets
• CO2 Incubators • Thermo Scientific™ ART™ Wide
• Biological Safety Cabinets
• Thermo Scientific™ ART™ Barrier Bore Tips

Pipette Tips • Benchtop Centrifuges
• Benchtop Centrifuges

Characterise

Multiple methods from basic to more advanced characterisation are required throughout as validation is critical in (iPSC) research

thermofisher.com/detectpsc

Invitrogen™ EVOS™ Cell Imaging Systems Thermo Scientific™ CellInsight™ CX7 LZR High Content Analysis (HCA) platformH
Invitrogen™ Attune™ NxT Flow CytometerH Immunocytochemistry and live staining kitsH
Applied Biosystems™ TaqMan® hPSC Scorecard™ Assay Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
Invitrogen™ Countess™ II and Countess™ II FL Automated Cell Counters• Invitrogen™ GeneArt™ Genomic Cleavage Selection Kit
Thermo Scientific™ Varioskan™ LUX Multimode Microplate Readers Invitrogen™ Antibodies for Stem Cell Research
Thermo Scientific™ Multiskan™ Sky Microplate SpectrophotometerH
Stem Cell Research Home Page thermofisher.com/stemcells
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Cell Engineering Workflow

An efficient and streamlined workflow for all applications

Cell culture and Design, build and Genome editing Clone validation Cell analysis and
characterisation deliver detection screening
Validate selected successfully
A complete genetic understanding Bioinformatically design and build Screen and enrich cell populations edited clones Cell analysis & screening using
of the starting cell population – your gene edit. Highly efficient for further analysis validated cell assays to determine
setting the baseline transfection and analysis the significance of the successful
genome edit

• Gibco™ cell culture media and •• Gibco™ cell culture media •• Invitrogen™ GeneArt™ Genomic •• Applied Biosystems™ • Cell-based assays and services
reagents Cleavage Detection Kit SeqStudio™ Genetic Analyzer • Invitrogen™ EVOS™ Cell Imaging
• Transfection reagents for DNA and • Applied Biosystems™
• Thermo Scientific™ Nunc™ cell RNA (Invitrogen™ Lipofectamine™ H• Invitrogen™ SuperScript™ IV Systems
culture flasks, plates, dishes, conical 3000, MessengerMAX™, VILO™ Master Mix QuantStudio™ Real-Time PCR • Invitrogen™ Countess™
tubes and serological pipettes RNAiMAX and CRISPRMAX™ • Applied Biosystems™ TaqMan® Systems
reagents) II FL Automated Cell Counters
• Applied Biosystems™ MagMAX™ Real-Time PCR H• Invitrogen™ PrimeFlow™ RNA • Thermo Scientific™ CellInsight™
DNA and RNA Kits for purification • Invitrogen™ Neon™ Transfection • Ion Torrent™ Next-Generation Assays
System • Invitrogen™ QuantiGene™ Assays CX7 LZR HCA Platform
• Ion Torrent™ Next-Generation Sequencing Systems • Thermo Scientific™ Nunc™ cell-
Sequencing • Design tool utilising state-of- • Applied Biosystems™ Laboratory Essentials:
the-art algorithm built to search • Standard Cryotubes based assays microplates
• Applied Biosystems™ TaqMan® qPCR predesigned and design de novo HSeqStudio™ Genetic • CO2 Incubators • Applied Biosystems™
genotyping reagents and assays CRISPRs and TALs • Biological Safety Cabinets
Analyzer • Benchtop/Floor Model Centrifuge QuantStudio™ Sequencing
• Invitrogen™ Dynabeads™ Magnetic • Invitrogen™ TrueGuide™ • Thermo Scientific™ F1-ClipTip™ Systems
Beads for cell isolation and expansion Laboratory Essentials: • Ion Torrent™ Ion S5 and S5XL™
HSynthetic Guide RNA and • Standard Cryotubes Manual Pipette Sequencing Systems
• Thermo Scientific™ KingFisher™ • Reporter assays
Magnetic Particle Processors for TrueCut™ Cas9 v2.0 • Thermo Scientific™ Varioskan™ LUX
automated cell isolation Multimode Microplate Readers
Laboratory Essentials:
Laboratory Essentials: • Ultra-Low Temperature Freezers H• Invitrogen™ Attune™ NxT Flow
• Thermo Scientific™ Nalgene™ • Super/Ultra Centrifuges Cytometer
• Large Capacity Centrifuges • Invitrogen™ Molecular Probes™
Rapid-Flow™ Filters • Benchtop Centrifuges
• CO2 Incubators • Thermo Scientific™ E1-ClipTip™ reagents
• Water Baths • Invitrogen™ primary and secondary
• Water Purification Systems Electronic Pipette
• Biological Safety Cabinets • Vacuum Concentrators antibodies, eBioscience™ and
• Standard Cryotubes/2D Tubes functional reagents
• Thermo Fisher Scientific™ • Thermo Scientific™ Nunc™ cell-
based assays
Multidrop™ Reagent Dispensers • Western Blot analysis
• Microbiological Incubators
• Environmental Chambers Laboratory Essentials:
• pH Meters • Standard Cryotubes
• Biological Safety Cabinets
• Benchtop/Floor Model Centrifuge

thermofisher.com/geneedit l thermofisher.com/geneengineeringebook

Let us deliver your engineered cells with custom services thermofisher.com/customservices

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Gene-Modified T Cell Manufacturing Workflow

Streamline the path from discovery to cure - cell therapy solutions for every step

Cell isolation Cell engineering Cell expansion Characterise Supply chain
and activation and regulatory
Gene delivery, modification, The cell expansion process is critical to generating the highest- Multiple methods from
Isolation and activation vector integration and viral quality CAR T cell product basic to more advanced Resources to support
of T cells production and purification characterisation are required best practices when
technologies throughout the process of manufacturing gene-modified
manufacturing gene-modified T cells
T cells

• Gibco™ CTS™ (Cell Therapy Gibco™ products: Gibco™ products: Invitrogen™ products: • Manufacturing Support
Systems) Dynabeads™ CD3/ • LV-MAX™ Lentiviral • CTS™ OpTmizer™ T Cell Expansion SFM • Countess™ II FL Automated Documents, Regulatory
CD28 • CTS™ Immune Cell SR Support Files
Production System • CTS™ Growth Factors Cell Counter
• Invitrogen™ CTS™ • CTS™ Synth-a-Freeze™ Medium • USA Drug Master files
DynaMag™ H• CTS™ LV-MAX™ Lentivirus H• Attune™ NxT Flow and European Regulatory
Production System thermofisher.com/CTS Cytometer Support Files
• Invitrogen™ DynaMag™ • Ion Torrent™ Next-
Invitrogen™ products: Laboratory Essentials: thermofisher.com/support
Laboratory Essentials: • Neon™ Transfection System Generation Sequencing
• General Purpose Centrifuges • CRISPR-Cas9™ Technology • CO2 Incubators • Fisher Bioservices Cold chain
• Centrifuge Tubes • GeneArt® TAL™ Effectors • Biological Safety Cabinets Applied Biosystems™ logistics
• Thermo Scientific™ • Thermo Scientific™ S1 Pipette Fillers products:
Products and Services • MycoSEQ™ Mycoplasma fisherbioservices.com/
ClipTip™ Pipettes coldchainlogistics
Laboratory Essentials: Detection Kit, with
• Thermo Scientific™ Discriminatory Positive
Control
ART™ Wide Bore Tips • MycoSEQ™ Mycoplasma
• Benchtop Centrifuges Detection Kit, with
• Lipofectamine transfection Discriminatory Positive
Control & sample preparation
reagents
• Nunc™ Cell Factories Laberatory Essentials:
• Orbital Shakers • Immuno Plates
• CO2 Incubators • Storage Plates

Transition your cell therapy to the clinic with confidence. thermofisher.com/celltherapy

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Gene Expression Workflow

Integrated solutions and flexible formats for DNA and RNA analysis

Sample collection / RNA extraction and cDNA synthesis Real-Time PCR Data analysis
cell culture quantification
cDNA synthesis products designed From single tubes to qPCR array Remotely analyse your data sets,
Robust cell growth using DNA or RNA purification and for superior performnce in RT-PCR formats – a throughput for every securely store data, or share results with
proven media, combined with stabilisation from a variety of samples applications lab’s needs your colleagues online using Thermo
complementary culture formats. like tissue, cells, blood Fisher Connect (using Thermo Fisher
Invitrogen™ products: Connect is optional)
• Gibco™ cell culture media and Can be skipped using Direct PCR • SuperScript™ IV VILO Master
reagents products Applied Biosystems™ products: Applied Biosystems™ products:
HMix • TaqMan® Gene Expression • TaqMan® Genotyper Software
• Thermo Scientific™ Nunc™ cell Invitrogen™ products: • CopyCaller™ Software
culture flasks, plates, dishes, conical • PureLink™ Kits for RNA and • SuperScript™ IV VILO Master HAssays • AlleleTyper™ Software
tubes and serological pipettes • DataAssist™ Software
HDNA HMix with ezDNase Enzyme • TaqMan® Advanced miRNA • RealTime™ StatMiner Software
thermofisher.com/cellculture • Expression Suite Software
Laboratory Essentials: • Invitrogen Fast Advanced Cells thermofisher.com/4vilo HAssays
• GP Centrifuges Laboratory Essentials: thermofisher.com/connect
• CO2 Incubators Hto CT Kits • Biological Safety Cabinets • TaqMan® Array Cards
• Standard Cryotubes • TaqMan® OpenArray Plates
• Liquid Transfer Products thermofisher.com/ sampleprep • TaqMan® Fast Advanced Master
• Orbital Shaker • Qubit™ 4 Fluorometer for accurate
• Thermo Scientific™ Nalgene™ HMix
HDNA and RNA quantification
Rapid-Flow™ Filters • PowerUp™ SYBR™ Green Master Mix
• Thermo Scientific™ Nalgene™ • Thermo Scientific™ Multiskan™
Sky Microplate Spectrophotometer thermofisher.com/taqman
Syringe Filters for medium to high throughput
• Centrifuge Bottles H• QuantStudio™ 1 System
• Thermo Scientific™ S1 Pipette HDNA and RNA quantification
• QuantStudio™ 3 System
Filler thermofisher.com/qubit4 • QuantStudio™ 5 System
• Microbiological Incubators • Thermo Scientific™ KingFisher™ • QuantStudio™ 6 Flex system
• Environmental Chambers • QuantStudio™ 7 Flex system
Magnetic Particle Processors • QuantStudio™ 12k Flex system
• Applied Biosystems™ MagMAX™ • QuantStudio™ 5 Dx System
• QuantStudio™ 3D for digital PCR
HMirvana total RNA kit
thermofisher.com/qsfamily
Laboratory Essentials:
• Storage Plates Laboratory Essentials:
• Water Purification Systems • Vacuum Concentrators
• Super/Ultra Centrifuges • Thermo Scientific™ E1- ClipTip™
• Thermo Scientific™ Thermal Mixer
• Microcentrifuges Electronic Pipette
• 2D Tubes • Thermo Scientific™ ClipTip™
• Readers and Cappers/Decappers
• Thermo Scientific™ F1-ClipTip™ Extended Length Tips

Manual Pipettes

For transcriptomic solutions in microarrays, next-generation sequencing and qPCR visit thermofisher.com/idealmatch

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Endpoint PCR Workflow

Trusted solutions for your entire nucleic acid detection and analysis workflow

Extraction Quantification cDNA synthesis Amplification Electrophoresis

DNA or RNA purification and Specific fluorescence-based Reverse transcription products Wide range of PCR enzymes, Nucleic acid electrophoresis
stabilisation from a variety of detection of nucleic acids available in variety of formats to suit plastic consumables, instruments, gels, stains, and
samples like tissue, cells, blood any application oligonucleotides, and instruments ladders
Can be skipped using Direct PCR
products

Invitrogen™ products: Invitrogen™ products: Invitrogen™ products: Invitrogen™ products: Invitrogen™ products:
• Qubit 4™ Fluorometer and Starter • SuperScript™ IV Reverse • Platinum™ II Taq Hot-Start DNA • E-Gel™ Power Snap
H• PureLink™ Kits for RNA and DNA
HKits HTranscriptase HPolymerase HElectrophoresis System
• Fast Advanced Cells to CT H• Qubit™ 4 RNA IQ Kit
• SuperScript™ IV First-Strand • Platinum™ SuperFi™ DNA • E-Gel™ CloneWell™ II,
HKits • Qubit™ 4 RNA Kits SizeSelect™ II, and EX Agarose
• Qubit™ 4 DNA Kits HSynthesis System HPolymerase Gels
thermofisher.com/ sampleprep • Quant-iT™ Kits
• SuperScript™ IV One-Step RT- thermofisher.com/platinum • TrackIt™ Ladders
• KingFisher™ Magnetic Particle Thermo Scientific™ products: • UltraPure™ Agarose
Processors • Fluoroskan™ MicroPlate Reader HPCR System Applied Biosystems™ products: • SYBR™ Safe and SYBR™ Gold
thermofisher.com/qubit4 • SimpliAmp™ Thermal Cycler
• MagMAX™ mirVana™ Total RNA thermofisher.com/superscript • Veriti™ Thermal Cycler Dyes
• ProFlex™ PCR System
HIsolation Kit Laboratory Essentials: • MicroAmp™ plastic consumables thermofisher.com/electrophoresis
• Standard Cryotubes Laboratory Essentials:
Laboratory Essentials: thermofisher.com/thermalcyclers • Thermo Scientific™ E1-ClipTip™
• Benchtop Centrifuges
• Standard Cryotubes • Custom DNA Oligos Electronic Pipette
• Storage Plates • Random Primers • Thermo Scientific™ Owl™ Gel
• Multi-Tube Rotators • OligodT Primers
• Dry Bath Systems and Power Supplies
• Heating Cooling Drybath thermofisher.com/oligos
• Heating Shaking Drybath
Laboratory Essentials:
• Themo Scientific™ F1-ClipTip™

Manual Pipette

• Thermo Scientific™ ClipTip™
Extended Length Tips

• Vacuum Concentrators

Molecular biology educational resources thermofisher.com/mbschool

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DNA Variant Detection Workflow

Sequencing solutions from sample preparation to data

Sample collection / DNA extraction and Library preparation Sequence analysis DNA fragment
cell culture quantification analysis
Perform whole genome sequencing or targeted sequencing by Analyse DNA sequences
Robust cell growth using These kits deliver proven selecting your library preparation method across species to find genetic Analyse DNA fragment sizes
proven media, combined high quality DNA samples for variants of all types for genotyping, bacteria
with complementary culture sequence analysis identification; plant screening
formats or gene expression profiling

• Gibco™ cell culture media • Ivitrogen™ PureLink™ Ion Torrent™ next generation sequencing Next-generation sequencing Capillary electrophoresis
and reagents Genomic DNA Kits for viral • Ion Xpress™ Plus Fragment Library Kit Ion Xpress™ Barcode Adapters • Ion Torrent™ Ion fragment analysis
DNA Applied Biosystems™
• Thermo Scientific™ Nunc™ 1-96 Kit •GeneStudio™ S5 products:
cell culture flasks, plates, • Applied Biosystems™ • SeqStudio™ Genetic
dishes, conical tubes and MagMAX™ DNA extraction Ion Torrent™ kits for targeted sequencing, any species: Sequencing Systems
serological pipettes Kits, human DNA • Ion Ampliseq™ Library Kit Plus, HD Library Kit, HD Dual Barcode Kit 1-24 HAnalyzer
• Ion AmpliSeq™ Kit for Chef DL8 for automated gene panel library preparation Capillary electrophoresis
Laboratory Essentials: • Thermo Scientific™ Sanger sequencing • 3500 Series Genetic
• GP Centrifuges GeneJET™ kits for plasmid Ion Torrent™ kits and panels for target amplification Applied Biosystems™ Analyzers (8,24 capillaries)
• CO2 Incubators DNA preparation • Ion TargetSeq™ Exome Kit for whole exome sequencing products:
• Standard • Ion AmpliSeq™ Exome Kits for coding region exome sequencing • SeqStudio™ Genetic • 3730xl Genetic Analyser
Thermo Scientific™ products: • Ion AmpliSeq™ gene panel portfolio for targeted gene sequencing (48 or 96 capillaries)
Cryotubes/2DTubes • KingFisher™ Magnetic • Ion AmpliSeq™ HD gene panels for high sensitivity gene panel sequencing HAnalyzer
• Liquid Transfer Fragment analysis kits for
• Orbital Shakers Particle Processors Automated library preparation systems • 3500 Series Genetic CE
• Water Baths for automated sample • Ion Torrent™ Ion Chef™ System for automated sequencing library, Analyzers Applied Biosystems™
• Thermo Scientific™ preparation products:
sequencing preparation • 3730xl Genetic Analyzer • GeneScan Size Standards
Nalgene™ Rapid-Flow™ Laboratory Essentials: • Applied Biosystems™ Next-Generation Sequencing library builder system • SNaPshot™ Multiplex
Filters • SuperSpeed/Ultra/Micro • Applied Biosystems™ capillary electrophoresis Sanger Sequencing
• Biological Safety Cabinets • Primer Designer Tool for target amplification System for Multiplex SNP
• Manual Pipettes Centrifuges • Applied Biosystems™ Sanger Sequencing Kit analysis
• Microbiological Incubator • Standard Cryotubes/2D Tubes • Applied Biosystems™ Exo-SAP-IT™ Express PCR Product Cleanup Reagents
• Environmental Chamber • GP Centrifuges • Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kits, Laboratory Essentials:
• Storage Plates • Themo Scientific™ Owl™
• Thermo Scientific™ Terminator v3.1 Cycle Sequencing Kits, Terminator v1.1 Cycle
Sequencing Kits, and XTerminator Purification Kit Large Gel Systems
Multidrop™ Reagent • Capillary and capillary arrays
Dispenser • Polymers
• Thermo Scientific™ E1- • Collibri™ Library Preparation Products for Illumina™ Systems
ClipTip™ Equalizer Pipette
• Multi-Tube Rotators H• Collibri™ Library Quantification Kit
• Dry Bath H• Platinum™ SuperFi™ Library Amplification Master Mix
H• Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems

Whole transcriptome analysis
• Applied Biosystems™ Clariom™ Microarrays

Help your lab realise its full sequencing potential thermofisher.com/sequencing

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Molecular Cloning Workflow

A complete toolset for your cloning workflow – from restriction enzymes to gene
synthesis products

DNA preparation Gene synthesis Construct creation Transformation Colony screening

Can be avoid if using E-Gel Power Synthetic gene fragments and fully Efficient tools to create accurate Multiple choices for efficient Quickly identify correct construct-
Snap with the E-gel Clonwell II sequenced and cloned plasmid constructs in less time transformation containing clones
Agarose gel constructs

Invitrogen™ products: • Ready to clone Invitrogen™ Invitrogen™ products: Invitrogen™ products: Invitrogen™ products:
• E-Gel™ Power Snap Electrophoresis • TOPO™ cloning system, TA • Chemical and electrocompetent E.coli • E-Gel™ Power Snap Electrophoresis
•GeneArt™ Strings™ DNA
HSystem Cloning™ Kits, Gateway™ System cells HSystem
Fragments • Anza™ Restriction and Cloning
• E-Gel™ CloneWell™ II Agarose • Invitrogen™ GeneArt™ Gene H• Multishot™ Competent Cells • E-Gel™ CloneWell™ II, SizeSelect™ II,
Enzymes and EX Agarose Gels
HGel •Synthesis service: Optimised, ready- • Platinum™ SuperFi™ DNA thermofisher.com/cloning
• Platinum™ II Taq Hot-Start DNA
Laboratory Essentials: to-use and sequenced plasmids HPolymerase
• Microcentrifuges thermofisher.com/genesynthesis HPolymerase
• Standard Cryotubes thermofisher.com/cloning
• Thermo Scientific™ Finnpipette™ Laboratory Essentials: • Applied Biosystems™ SeqStudio™ Laboratory Essentials:
• Standard Cryotubes • Standard Cryotubes
F2 Manual Pipette HGenetic Analyzer
• ART™ Barrier tips
• Dry Bath
• Vacuum Concentrators
• Heating Cooling Drybath
• Heating Shaking Drybath

Select the cloning technology that the best meets your needs at thermofisher.com/choosecloningmethod

• Restriction Enzyme Digestion & Ligation Cloning— • Gateway™ Cloning Technology—for the greatest flexibility in vector • Type IIs Assembly—for simultaneous cloning of up to 8 DNA
an industry standard for molecular biologists choices and downstream applications fragments without scars

• TOPO™ Cloning—for simple, fast, and convenient cloning • Seamless Cloning and Assembly—for simultaneous cloning of up to 4 • GeneArt™ Gene Synthesis and Strings™ DNA Fragments —for the
DNA fragments highest convenience and 100% accuracy

• HBest seller New product 11

Protein Expression Workflow

Cloning Plasmid purification Delivery Expression Protein analysis

Getting a 100% in frame sequence Isolation of high quality plasmid Maximise transfection efficiency Protein expression systems for the Determine protein quality and
verified plasmid vector that can DNA that can be used for delivery with these tailored solutions best yield and quality quanitity
express the selected protein and transfection into the chosen
effectively in your cell expression cellular expression system Gibco™ products: •• Gibco™ ExpiCHO™ Kit • Invitrogen™ iBind™ Western
system (to see the full workflow refer Systems
to page 11) • Transfection grade: Invitrogen™ •• ExpiCHO™ Kit. • Gibco™ Expi293™ System
• Invitrogen™ Alexa Fluor™ Plus
• Ready to clone Invitrogen™ •PureLink™ HiPure and Fast • Expi293™ System •• Gibco™ ExpiSF™ System Secondary Antibodies
GeneArt™ Strings DNA Fragments • Invitrogen™ iBright™ Imagers
Hplasmid purification Kits •• ExpiSF™ System • Invitrogen™ MembranePro™ Kit • Thermo Scientific™SuperSignal™
• Invitrogen™ GeneArt™ Gene • Invitrogen™ PichiaPink™ System
Synthesis service: Optimised, ready- • Endotoxin free: Invitrogen™ Expi Invitrogen™ products: • Invitrogen™ Baculovirus System Substrates
to-use 100% sequence verified • Lipofectamine™ 3000 Reagent • Thermo Scientific™Multiskan™
HPureLink™ Kits • MembranePro™ Kit Laboratory Essentials:
thermofisher.com/genesynthesis • Champion™ pET System • CO2 Incubators Sky Microplate
Laboratory Essentials: • PichiaPink™ System • Orbital Shakers Spectrophotometer for medium
• Invitrogen™ TOPO™ Cloning • SuperSpeed/Ultra Centrifuges • Baculovirus System to high throughput protein
System, TA Cloning Kits, • Standard Cryotubes • Neon™ Transfection System
Invitrogen™ Gateway™ System • Thermo Scientific™ Nalgene™ Laboratory Essentials: Hquantitation
• Plate Readers and Microplates
• Invitrogen™ Anza™ Restriction and Rapid-Flow Filters • Liquid Handling Products Laboratory Essentials:
Cloning Enzymes • Water Baths • Biological Safety Cabinets • 2D Tubes
• Incubators & CO2 Shakers • Barcode Readers and Cappers/
• Invitrogen™ Platinum™ SuperFi • GP Centrifuges
• Storage Plates Decappers
HDNA Polymerase • Thermo Scientific™ F1 ClipTip™

• Chemical and electrocompetent Manual Pipette
E.coli cells • Heating Cooling Drybath
• Hybridization Ovens
thermofisher.com/cloning • Vacuum Concentrators

• Invitrogen™ Champion™ pET System

Laboratory Essentials:
• Standard Cryotubes
• Water Purification Systems
• Open Air Shakers
• Pipettes
• Compact Digital Rocker

Invitrogen GeneArt Gene-to-Protein service thermofisher.com/genetoprotein

• The most convenient way to receive the desired protein in a short • The optimised gene in a suitable expression vector for further in-house • Dedicated and experienced project management office team with very
amount of time work close communication in all project phases

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Protein Analysis Application

Combined tool set with optimised, validated systems

Isolation Separation Transfer Detect Quantify
and clean-up
Pour-your-own protein gel system, Buffers, membranes and blotting Western blotting reagents, primary Assay, deplete, calibrate and label
Chromatography media, dialysis, precast protein gels, standards, systems and secondary antibodies,
desalting, concentrations devices tanks and staining systems chemiluminescent substrates,
and magnetic beads imaging systems and cell counters

Thermo Scientific™ products: Invitrogen™ products: Thermo Scientific™ products: Invitrogen™ products: Thermo Scientific™ products:
• LC-MS • SureCast™ Gel Handcast System • Nitrocellulose Membranes • iBind™ Western Systems • Pierce™ Rapid Gold BCA and
• PVDF Membranes • Alexa Fluor™ Plus Secondary
•• Poros™ Chromatography Media •• Novex™ WedgeWell™, Bolt™ and Coomassie Protein Assays
NuPAGE™ precast gels Invitrogen™ products: HAntibodies • Varioskan™ LUX and Fluoroskan™
• Pierce™ Cell Lysis Solutions • iBlot™2 Blotting System H• iBright™ Imagers
•• Mini Gel Tank • Power Blotter and Power Blotter Microplate Readers
•• Slide-A-Lyzer™ Dialysis Products • EVOS™ Cell Imaging Systems • Thermo Scientific™
•• Zeba™ Desalting Columns Thermo Scientific™ products: XL H • Countess™ II FL Automated Cell
Multiskan™ Sky Microplate
• PES Concentrators Tubes •• PageRuler™ and Spectra™ Protein thermofisher.com/transfer Counters Spectrophotometer for medium
Ladders Thermo Scientific™ products: to high throughput protein
Applied Biosystems™ products: Laboratory Essentials: • SuperSignal™ Substrates
• KingFisher™ Magnetic Particle thermofisher.com/separate Hquantitation
• Buffers thermofisher.com/antibodies
Processors Laboratory Essentials: thermofisher.com/detect •• TMT™ Isobaric Labeling ,
• Vacuum Concentrators • Blotting Systems Proteases, Peptide enrichment,
Invitrogen products: • Thermo Scientific™ Owl™ Gel Laboratory Essentials: Calibration Solutions, Standards for
• Hybridisation Ovens mass spectrometry
•• Novex™ Dynabeads™ Systems and Power Supplies
Immunoprecipitation Invitrogen™ products:

thermofisher.com/transfer H• Qubit™ 4.0 Fluorometer

Laboratory Essentials: • ELISA Kits and ProcartaPlex™
• SuperSpeed/Ultra Centrifuges multiplex assays
• Standard Cryotubes
• Thermo Scientific™ Nalgene™ • ProQuantum™ High-sensitivity

Syringe Filters Himmunoassay
• Thermo Scientific™ Nalgene™
thermofisher.com/proteinassays
Rapid-Flow Filters
• Thermo Scientific™ E1-ClipTip™

Equalizer Pipette
• Thermo Scientific™ Multidrop™

Reagent Dispenser

Uncover your full protein potential thermofisher.com/protein

• HBest seller New product 13

Cell Analysis Application

A complete toolset for your cell analysis workflow

Observe Count Visualise Analyse – Analyse –
Flow Cytometry Quantitative Imaging
All-in-one transmitted-light systems Accurate and automated counting, Designed for multi-colour
that easily fit in the cell culture hood monitoring of fluorescent cell fluorescence detection of cells and Run multi-colour samples faster Integrate high content analysis
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Invitrogen™ products: sample loss due to clogging
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