1 Table of Contents 1. A STUDY ON THE KNOWLEDGE, ATTITUDE, PRACTICE AND SELF- PERCEPTION ON PERIODONTAL DISEASE AMONG THE OLDER ADULT POPULATION IN MALAYSIA................. 2 2. ASSOCIATION OF PERCEIVED STRESS, XEROSTOMIA SEVERITY, AND PERIODONTAL STATUS AMONG UNDERGRADUATE DENTAL STUDENTS ..................................................... 3 3. THE ASSOCIATION BETWEEN SYSTEMIC DISEASES AND PERIODONTITIS AMONG PATIENTS REFERRED TO A GOVERNMENT PERIODONTAL SPECIALIST CLINIC IN MELAKA, MALAYSIA.......................................................................................................................... 4 4. EXPRESSION OF INFLAMMATORY AND HEALING BIOMARKERS FOLLOWING USE OF ANTIMICROBIAL PEPTIDE GEL AS AN ADJUNCT TO SCALING AND ROOT SURFACE DEBRIDEMENT: A PILOT STUDY.......................................................................................... 5 5. AWARENESS, KNOWLEDGE, ATTITUDE AND ORAL HYGIENE PRACTICES TOWARDS PERIODONTAL HEALTH AMONG HEALTHCARE OFFICERS IN PERLIS ..................................... 6 6. ANTIBACTERIAL EFFECT OF METHANOLIC EXTRACTION (GAMMA AND NON-GAMMA IRRADIATION) OF TUALANG AND MANUKA HONEY ON STREPTOCOCCUS MUTANS AND PORPHYROMONAS GINGIVALIS ......................................................................................... 7 7. PATIENTS’ SATISFACTION AND QUALITY OF LIFE AFTER DENTAL IMPLANT REHABILITATION: A CROSS-SECTIONAL STUDY.................................................................... 8 8. THE EFFICACY OF AIR POLISHING IN SUPPORTIVE PERIODONTAL THERAPY AND IMPLANT MAINTENANCE: A SYSTEMATIC REVIEW AND META ANALYSIS ........................................... 9 9. A CLINICAL CASE REPORT ON MANAGEMENT OF AMLODIPINE-INFLUENCED GINGIVAL ENLARGEMENT IN A HYPERTENSIVE PATIENT................................................................... 10 10. THE EFFECT OF TUALANG HONEY ON VIABILITY OF HUMAN GINGIVAL FIBROBLAST (HGFS)............................................................................................................................. 11 11. IMPACT OF NONSURGICAL PERIODONTAL THERAPY ON ORAL HEALTH RELATED QUALITY OF LIFE IN PRIMARY DENTAL CARE PATIENTS: A PROSPECTIVE COHORT STUDY.. 12 12. CLINICAL AND METAGENOMIC INVESTIGATION OF LOCALLY DELIVERED SYNTHETIC ANTIMICROBIAL PEPTIDE IN NON-SURGICAL PERIODONTAL TREATMENT: A PILOT STUDY 13 13. LONGITUDINAL CHANGES IN MASTICATORY FUNCTION FOLLOWING NON-SURGICAL TREATMENT OF STAGE III AND IV PERIODONTITIS: A LONGITUDINAL OBSERVATIONAL STUDY ............................................................................................................................. 14
2 1. A STUDY ON THE KNOWLEDGE, ATTITUDE, PRACTICE AND SELF- PERCEPTION ON PERIODONTAL DISEASE AMONG THE OLDER ADULT POPULATION IN MALAYSIA Annabel Thomas1, Haslina Taib2, Zurairah Berahim2, Siti Lailatul Akmar Zainuddin2, Munirah Mohd Adnan2 Aim: To assess the knowledge, attitude, practices and self-perception of periodontal disease and its relationship with demographic factors among the older adult population in Malaysia. Methodology: This is a cross-sectional study, a questionnaire based, among the elderly adult population in Malaysia. A validated questionnaire was distributed through various social media platforms to assess the level of knowledge, attitude, oral hygiene practices and self-perception of periodontal disease. Results: A total of 397 respondents with a mean age and standard deviation (SD) of 64.32 (8.0) who fulfilled the criteria took part in this study. Those in the age range of 60 to 69 years were the highest respondents (45.8%). Male respondents were higher than female respondents (53% and 47% respectively) and the majority of them were Malays (33.8%). About a quarter of adults reported ‘bleeding gums’ while brushing their teeth, with females (51.5%) being more than males (48.5%). However, significant correlations were observed with gender, ethnicity, education and occupation. Men (57%) and women (43%) showed more negative attitudes toward the bilateral relationship between systemic disease and gum disease. They acknowledged the fact that poor oral hygiene can affect general health. Analysis of the frequency of dental examinations showed a significant association (p <0.05) seen in relation to tertiary education (57.9%) and retirees (38.8%). In all variables, a more negative response was noted with the use of dental aids such as toothpicks, dental floss and interdental brushes. A similar response was noted with the use of mouthwash with a significant association noted with ethnicity (p = 0.027). When assessing the presence of gum disease, a more negative response was noted with a significant association in relation to higher education (52.4%). Conclusion: Therefore, knowledge, attitude, practices and self -perception of periodontal disease among older adults are at a relatively low level. Therefore, more dental health education is needed to improve periodontal health among this group. ___________________________________________________________________________________________ 1 Periodontics Unit, Klinik Pakar Pergigian Bandar Mentakab, Jalan Karak, 28400 Mentakab, Pahang. 2 Periodontics Unit, School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan. Corresponding Author: Annabel Shanta a/p Edwin Thomas, Periodontics Unit, Klinik Pakar Pergigian Bandar Mentakab, Jalan Karak, 28400 Mentakab, Pahang. Phone: 09-2771841. E-mail: [email protected] Status of research: Completed during postgraduate study at Universiti Sains Malaysia
3 2. ASSOCIATION OF PERCEIVED STRESS, XEROSTOMIA SEVERITY, AND PERIODONTAL STATUS AMONG UNDERGRADUATE DENTAL STUDENTS Engku Ahmad Muzhaffar1,2, Zurairah Berahim1, Norsila Abdul Wahab 3, and Norkhafizah Saddki4 Aim: To invesWgate the associaWon of perceived stress, xerostomia severity, and periodontal status among undergraduate dental students. Materials and Methods: This was a two-phase cross-secWonal study. A total of 245 UniversiW Sains Malaysia (USM) undergraduate dental students parWcipated in Phase 1 that involved compleWon of validated self-administered quesWonnaires. The Perceived Stress Scale (PSS-10) and Summated Xerostomia Inventory (SXI) were used to assess perceived stress and xerostomia severity, respecWvely. In Phase 2, a random sample of 150 students from Phase 1 were selected for periodontal screening to determine their periodontal health status using the Community Periodontal Index (CPI). The data were analysed using SPSS v24.0. Results: The mean PSS-10 and SXI scores were 19.6 (SD 5.47) and 7.9 (SD 2.04), respecWvely. Malay, female, students who were unsaWsfied with their decision of pursuing denWstry, and those who lived off campus had significantly higher perceived stress. Students who were currently taking medicaWon had significantly more severe xerostomia. Only 7.3% of students had healthy periodonWum while 17.3% had gingival bleeding, 65.3% had calculus, 6.7% had shallow pockets, and 3.3% had deep pockets. The associaWon of perceived stress, severity of xerostomia, and periodontal status was not significant. Nevertheless, a significant posiWve correlaWon was found between PSS-10 and SXI scores (r=0.318, p<0.01). Conclusions: Most USM undergraduate dental students were affected by some degree of periodontal disease, but it was not associated with perceived stress or severity of xerostomia. Students with higher perceived stress had more severe xerostomia. InformaWon from this study could be uWlised by the dental school in planning towards providing a stress-free training environment. 1 Periodontics Unit, School of Dental Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia 2 Periodontics Unit, Pusat Pakar Pergigian Seremban, Jalan Zaaba, 70100 Seremban, Negeri Sembilan, Malaysia 3 Basic and Medical Sciences Unit, School of Dental Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia 4 Dental Public Health Unit, School of Dental Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia Corresponding Author: Norsila Abdul Wahab, Basic and Medical Sciences Unit, School of Dental Sciences, UniversiX Sains Malaysia Health Campus, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia. Email: [email protected] Status of research: Presented at the 3rd Postgraduate Research Day, in 3-minute oral pitch presentaXon (clinical sciences) category, organised by USM School of Dental Sciences on 28 April 2019. Proceeding Xtled “Periodontal Status and Associated Factors among Dental Students”; presented at the 13th Asian Pacific Society of Periodontology (APSP) congress, in poster presentaXon category, organised by APSP on 28-29 September 2019. Proceeding Xtled “AssociaXon of Perceived Stress, Xerostomia Severity, And Periodontal Status in Undergraduate Dental Students” and manuscript accepted for publicaXon in Journal of DenXstry Indonesia. Currently in the process of minor correcXon.
4 3. THE ASSOCIATION BETWEEN SYSTEMIC DISEASES AND PERIODONTITIS AMONG PATIENTS REFERRED TO A GOVERNMENT PERIODONTAL SPECIALIST CLINIC IN MELAKA, MALAYSIA Koh Carmen1,Arlene Khaw Bee Hong2 Aim: To identify the prevalence of systemic diseases among patients referred to a newly established government periodontal specialist clinic and to analyse the association between the severity (staging) and the rate of progression (grading) of periodontitis with the presence of these systemic diseases. Materials and Methods: Records of all new patients within the first five years of the clinic’s operation were reviewed and their periodontitis status were classified according to the 2017 World Workshop Classification system. The association between the severity of periodontitis and presence of systemic diseases was analysed using Chi square test, while Fisher’s Exact test was used to determine the association between the rate of progression and presence of systemic diseases. Results: A total of 489 dental records were reviewed. We found that 40.1% of our patients suffered from systemic diseases. The two most common systemic diseases were cardiovascular disease (11.4%) and diabetes mellitus (9.4%). No significant association was found between the severity of periodontitis and presence of systemic diseases (p=0.376). However, we found a significant association between the rate of progression of periodontitis and systemic diseases (p=0.004). There was also a significant association between the rate of progression of periodontitis and cardiovascular diseases (p=0.001). Conclusions: The rate of progression of periodontitis is significantly associated with the presence of systemic diseases and cardiovascular diseases. This highlights that periodontitis is not a stand-alone disease. Instead, it reaches across a spectrum of other non-communicable diseases. The collaboration between medical and dental practitioners can further improve the management of this group of patients. 1 Klinik Pergigian Seri Tanjung, Ministry of Health, Melaka, Malaysia 2 Periodontics Specialist Unit, Klinik Pergigian Seri Tanjung, Ministry of Health, Melaka, Malaysia Corresponding Author: Koh Carmen, Klinik Pergigian Seri Tanjung, Ministry of Health, 76400, Melaka, Malaysia. E-mail: [email protected] Status of research: Submieed for publicaXon in Malaysian Dental Journal, under reviewing process.
5 4. EXPRESSION OF INFLAMMATORY AND HEALING BIOMARKERS FOLLOWING USE OF ANTIMICROBIAL PEPTIDE GEL AS AN ADJUNCT TO SCALING AND ROOT SURFACE DEBRIDEMENT: A PILOT STUDY Muhammad Hadi Mohd Hanifah1,2 , Syarida Hasnur Safii2 , Wan Izlina Wan Ibrahim WI3 and Muhammad Zamri Hussin2 Aim: To invesWgate the effect of a locally delivered syntheWc AnWmicrobial PepWde (AMP) gel as an adjunct to scaling and root surface debridement in Stage III periodonWWs paWents in relaWon to proteins and cytokines expression. Materials and methods: This in vivo pilot study of a 3-months duraWon involved subjects with Stage III periodonWWs (n= 2) Split-mouth design was employed to assign periodontal sites to the test and control groups randomly. Periodontal parameters (periodontal pocket depth [PPD] and clinical aeachment level [CAL]) were measured at baseline, 6-weeks and 12-weeks. Gingival crevicular fluid (GCF) were sampled from teeth with PPD of >6 mm using paper points at the same Wme points. AdjuncWve AMP gel was applied to the test sites following scaling and root surface debridement. GCF samples (n= 24) were subjected to proteomic analysis using liquid chromatography-mass spectrometry (LC-MS Orbitrap). Results: A total of 741 and 886 proteins were idenWfied in the GCF samples represenWng test and control groups, respecWvely. At 12-weeks, a higher number of proteins related to biological processes was found in the test group as compared to control group but with no significant difference (p > 0.05). No significant increase of fibronecWn expression and decrease of annexin A1 expression (p > 0.05) were observed over Wme (p > 0.05) following applicaWon of AMP. Conclusions: IdenWficaWon of proteins in the GCF samples using LC-MS resulted in idenWficaWon of proteins that may be involved in the healing process following non-surgical periodontal treatment. 1 Oral Health Division, Ministry of Health, Putrajaya, Malaysia 2 Department of RestoraXve DenXstry, University of Malaya, Kuala Lumpur, Malaysia 3 Department of Oral & Craniofacial Sciences, University of Malaya, Kuala Lumpur, Malaysia Corresponding Author: Muhammad Hadi Mohd Hanifah, Unit PeriodonXk Bandar Raub, Klinik Pergigian Bandar Raub, Jalan Cheroh Lama, 27600 Raub, Pahang, Malaysia. E-mail: [email protected] Status of research: Oral presentaXon at 14th Dental Postgraduate Conference, UniversiX Malaya, Kuala Lumpur, 22-23 Sept 2021; E-poster research presentaXon at EuroPerio10 (2022) and published in Journal of Clinical Periodontology Volume: 49 issue 10, page(s): 1090-1090. heps://doi.org/10.1111/jcpe.13636
6 5. AWARENESS, KNOWLEDGE, ATTITUDE AND ORAL HYGIENE PRACTICES TOWARDS PERIODONTAL HEALTH AMONG HEALTHCARE OFFICERS IN PERLIS Mabel Mun Yen Chi1, Mior Azrizal B M.Ibrahim2 Aim: To determine the awareness, knowledge, ajtude, and oral hygiene pracWces towards periodontal health among healthcare officers in Perlis. Materials and Methods: A cross-secWonal and self-administered quesWonnaire that contained six quesWons of socio-demographic and 24 quesWons in four domains: awareness, knowledge, ajtude and oral hygiene pracWces. Total 209 parWcipants, with 69.4% non-dental related, 46.9% have serviced duraWons ≥ six to 10 years, 69.4% female and 93.3% age ranging 20-44. Results: Highlighted findings in awareness, 29.7% have bleeding gums following tooth brushing/ flossing/ eaWng; 11.0% aware have loose teeth; 10.0 % aware have gum disease. In knowledge, 62.2% informed dental plaque is hard deposit on teeth; 13.9% do not heard of periodontal disease/ gum disease; 19.6% do not know diabetes is risk factor for gum disease; 55.5% do not know pregnant women with periodontal disease could be more likely to have preterm low birth weight delivery. In ajtude, 41.1% visit the denWst whenever they have teeth problems; 20.0% last dental visit was 2 years ago; 52.6% do not visit denWst regularly due to have no Wme. In oral hygiene pracWce, 8.13% brush their teeth only once a day; 48.3% brush their teeth less than two minute; 49.8% do not change their toothbrush regularly; 31.1% inform never floss their teeth. Conclusions: Awareness, knowledge, ajtude and oral hygiene pracWces sWll need to improve to the opWmal level among healthcare officers. CollaboraWon in between medical fraternity and dental fraternity to enhance the periodontal health conscious, then contribute good general health. 1 Unit Pergigian, Hospital Bahagia Ulu Kinta, Perak 2 Unit Pakar PeriodonXk, Klinik Pergigian Pauh, Perlis Corresponding Author: Mabel Mun Yen Chi, Unit Pergigian, Hospital Bahagia Ulu Kinta, 31250, Tg Rambutan, Perak Darul Ridzuan. E-mail: [email protected] Status of research: Preliminary findings was oral presented at 22TH NIH ScienXfic Conference 2021, online plalorm, period of 21/09/21-23/09/21 and poster presentaXon at 6th Hospital Kuala Lumpur Research Day held on 3/08/23 at Auditorium Utama Hospital Kuala Lumpur.
7 6. ANTIBACTERIAL EFFECT OF METHANOLIC EXTRACTION (GAMMA AND NONGAMMA IRRADIATION) OF TUALANG AND MANUKA HONEY ON STREPTOCOCCUS MUTANS AND PORPHYROMONAS GINGIVALIS Zhi Ling Ong1, Zurairah Berahim2, Suharni Mohamad3, Wan Nazatul Shima Shahidan3, Haslina Taib2, Mohamad Ezany Yusoff4, Haswati Abdullah4 Aims: The aim of this study is to evaluate the antibacterial effects of methanolic extracts of non- and gamma irradiation of Tualang honey and Manuka honey on Streptococcus mutans and Porphyromonas gingivalis. Methodology: Agar well diffusion, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) assay were used to test the antibacterial activity of non- and methanol extraction, non- and gamma irradiation of Tualang and Manuka honey on Streptococcus mutans and Porphyromonas gingivalis at different concentration. Manuka honey was used as control. Amoxycilin was used as positive control while distilled water was used as negative control. Results: The results showed that the Tualang honey (non-methanol extraction, non-gamma irradiation) exhibit antibacterial effect on Streptococcus mutans at MIC and MBC of 25% compared to Manuka honey (non-methanol extraction, non-gamma irradiation) that has MIC and MBC of 50% respectively. The non-gamma irradiation, methanolic extracted Tualang and Manuka honey, gamma irradiation methanolic extracted Tualang and Manuka honey have MIC and MBC of 25% on Streptococcus mutans. All tested honey samples demonstrated MIC and MBC of 25% on Porphyromonas gingivalis. Conclusion: The antibacterial activity of non-and methanol extraction, non-and gamma irradiation of Tualang and Manuka honey work against Streptococcus mutans and Porphyromonas gingivalis with the exception of gamma irradiation of Tualang and Manuka honey on Streptococcus mutans. _________________________________________________________________________________________________________________ 1 PeriodonXcs Unit, Klinik Pergigian Labis, Labis, Segamat, Johor, Malaysia 2 PeriodonXcs Unit, School of Dental Sciences, UniversiX Sains Malaysia, Health Campus, Kubang Kerian, Kelantan Malaysia 3 Basic and Medical Science Unit, School of Dental Science, UniversiX Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia 4 Craniofacial Sciences Lanoratory, School of Dental Science, UniversiX Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia Corresponding author: Zhi Ling Ong, Periodontics Unit, Klinik Pergigian Labis, Labis, Segamat, Johor. Phone: 012- 4906922. E-mail: [email protected] Status of research: Completed
8 7. PATIENTS’ SATISFACTION AND QUALITY OF LIFE AFTER DENTAL IMPLANT REHABILITATION: A CROSS-SECTIONAL STUDY Noor Asyikin Saad1, Nuzul Izwan2, Yuhaniz Ahmad Yaziz2, Enny Esdayanty Abdul Manab3 Aim: Perceiving patients’ satisfaction and improving patients’ quality of life is challenging. This study assessed the patient’s quality of life and satisfaction after receiving a dental implant rehabilitation. Materials and Methods: All patients with dental implants placed between 2015 and 2019 were included. A self-administered questionnaire consisted of a demographic, patient satisfaction assessment, and the short version of the Malaysian Oral Health Impact Profile, S-OHIP (M) questionnaire. The patients indicated their rate on a 5-point Likert scale from 0 to 4. Statistical analyses were made using the SPSS version 26, statistical significance was considered for p<0.05. Results: A total of 58 patients with 91 implants completed the questionnaire were included. In general, 56 (96.6%) participants were satisfied with the dental implant mainly with the speech function and maintenance procedure. The Cronbach’s Alpha coefficient for the nine items of satisfaction was 0.798. The mean OHIP-14 score was 2.1 (SD: 3.11; range from 0 to 12) with the highest score for psychological discomfort (mean: 0.92, SD: 1.35) and the lowest score for psychological disability (mean: 0.00, SD: 0.00). All domains of OHIP-14 had Coefficient of Variance, CV > 1 (ranged from 1.47 to 8.77) which considered being high variance. Conclusions: Overall, all participants had OHIP-14 scores ranging from 0 to 12 which can be considered a low score that indicated higher Oral Health-Related Quality of Life (OHRQoL). The correlation between patient satisfaction and quality of life is significant (P<0.05). Keywords: Oral Health Impact Profile (OHIP), Oral Health-Related Quality of Life (OHRQoL), dental implant satisfaction, dental implant rehabilitation. 1 Faculty of Dentistry, Universiti Teknologi Mara (UiTM), Selangor, Malaysia 2 Klinik Pergigian Pakar Periodontik Mak Mandin, Pulau Pinang, Malaysia 3 Pejabat Timbalan Pengarah Kesihatan (Pergigian) Pulau Pinang, Pulau Pinang, Malaysia Corresponding Author: Noor Asyikin Saad, Center of Periodontic Studies, Faculty of Dentistry, Universiti Teknologi Mara (UiTM), Sungai Buloh, Gombak 47000, Malaysia. E-mail: [email protected] Status of research: Published in the IIUM Journal of Orofacial and Health Sciences Volume: 4 Issue: 1, (February 2023) page(s): 47-58. https://doi.org/10.31436/ijohs.v4i1
9 8. THE EFFICACY OF AIR POLISHING IN SUPPORTIVE PERIODONTAL THERAPY AND IMPLANT MAINTENANCE: A SYSTEMATIC REVIEW AND META ANALYSIS Tan Shiuan Lee1, Nor Adinar Baharuddin2, Nor Shafina Mohamed Nazari2, TuN Ningseh Mohd-Dom3 Aim: PaWents who had undergone periodontal therapy and implant placement require long-term professional monitoring and periodic subgingival mechanical debridement during recall intervals. This systemaWc review aims to assess the clinical outcomes, paWents’ percepWon and cost effecWveness of repeated periodontal therapy with air polishing devices (APDs) in comparison with hand instruments and/or power-driven instruments in SupporWve Periodontal Therapy (SPT) and implant maintenance. Materials and Methods: Electronic and hand searches were performed to idenWfy clinical human trials with at least 6 months follow-up in SPT or implant maintenance published in English from 01 January 2000 to 30 April 2020. Results: Repeated intervenWons using APDs in SPT paWents resulted in beeer clinical outcome for probing pocket depth (PPD) reducWon. There was staWsWcally significant more PPD reducWon in SPT paWents following the use of APDs (weighted mean difference 0.14, 95% confidence interval -0.25 to - 0.02, p=0.02, I2 =6%). For reducWon of bleeding on probing (BOP) and gain in clinical aeachment level (CAL), the results were comparable with hand instruments and/or power-driven instruments. In terms of implant maintenance, APDs resulted in promising clinical outcomes for PPD and BOP reducWon whereas CAL gain was comparable to the convenWonal treatment. Treatment with APDs was associated with lower pain score based on the Visual Analogue Scale (VAS) and higher paWent acceptance in SPT studies. None of the implant maintenance studies recorded paWent reported outcome. No studies assessed and reported on economic evaluaWon of the intervenWon. Conclusions: There is evidence to support that repeated subgingival debridement using APDs in SPT has slight superiority in clinical parameter with reference to PPD reducWon and beeer paWents’ comfort, when compared to convenWonal treatments. There is limited evidence to show that repeated applicaWon of APDs has higher potenWal in improving clinical outcomes compared to convenWonal treatments in implant maintenance paWents. 1 Oral Health Division, Ministry of Health, Putrajaya, Malaysia 2 Faculty of DenXstry, UniversiX Malaya, Kuala Lumpur, Malaysia 3 Faculty of DenXstry, UniversiX Kebangsaan Malaysia, Kuala Lumpur, Malaysia Corresponding Author: Tan Shiuan Lee, Unit PeriodonXk, Klinik Pergigian Gunung Rapat, Jalan Gopeng, Ipoh, 31350, Perak, Malaysia. E-mail: [email protected], [email protected] Status of research: Oral presentaXon in 14th Dental Postgraduate Conference, UniversiX Malaya, Kuala Lumpur, 22-23 September 2021; E-poster research presentaXon in EuroPerio10 (2022); published in Journal of Clinical Periodontology, Volume 49, Issue 10, page(s): 1090-1090. heps://doi.org/10.1111/jcpe.13636 and published in the BMC Oral Health 22, 85 (2022). heps://doi.org/10.1186/s12903-022-02120-6
10 9. A CLINICAL CASE REPORT ON MANAGEMENT OF AMLODIPINE-INFLUENCED GINGIVAL ENLARGEMENT IN A HYPERTENSIVE PATIENT Pathmaprasath PathmamorNe1, Norjehan bt LaNb2 Introduc8on: Amlodipine, a commonly prescribed 3rd generaWon Calcium Channel Blockers(CCB) for hypertension & angina has been associated with Gingival Enlargement (GE) to some paWents. Gingival Enlargements (GEs) refer to an excessive growth of gingival sov Wssues. It is frequently observed as adverse effects with the use 3 major groups of drugs namely anWconvulsants, calcium channel blockers, and immunosuppressants. Clinical case: A 53-year-old paWent was referred to the PeriodonWcs Unit KP Kota Damansara for the management of swollen gums and mild pain for the past 1 month. PaWent has an underlying Hypertension, Diabetes Mellitus and under Amlodipine medicaWon. She presented with poor oral hygiene, gingival overgrowth over her upper anterior and lower anterior region and generalised bleeding gums. She received non-surgical periodontal therapy which included oral hygiene coaching and moWvaWon, full mouth scaling and root surface debridement. This followed by surgical open flap debridement and gingivectomy with biopsy done at upper anterior gingiva. Conclusions: Medical and Dental Health pracWWoners have to know the adverse effects of CCB which can be a aeWological factor for GE and able to pracWce care while prescribing the drugs and idenWfy changes in the oral cavity of such paWents. PaWents must be informed about the tendency of these drugs to cause GE and pracWce meWculous oral hygiene maintenance. Early diagnosis and treatment is important as it can prevent periodonWum damage and risk of loss of denWWon. __________________________________________________________________________________________ 1 Klinik Pergigian Branang, Hulu Langat, Selangor, Malaysia 2 PeriodonXcs Unit, Klinik Pergigian Kota Damansara, Selangor, Malaysia Corresponding Author: Pathmaprasath PathmamorXe, Klinik Pergigian Beranang, Jalan Sesapan Batu Rembau ,43700 Beranang, Hulu Langat, Selangor. Phone: 012-6911164. E-mail: [email protected] Status of research: Completed
11 10. THE EFFECT OF TUALANG HONEY ON VIABILITY OF HUMAN GINGIVAL FIBROBLAST (HGFS) Nor Azira Zahadi1,2 SiN Lailatul Akmar Zainuddin SLA2, Zurairah Berahim2 Aims: To determine the cytotoxicity of Tualang honey towards human gingival fibroblast (HGFs) in cell culture. Materials and methods: HGFs were cultured in standard medium in a 5% CO2 incubator at 37°C in a humidified atmosphere. The cells were seeded at a cell density of 5x103 cells/well in a 96-well culture plate for 24 hours. Tualang honey were diluted in 10%, 5%, 2.5%, 1.25%, 0.62%, 0.31% and 0.16% diluWons. Each concentraWon was tested for its toxicity using MTT assay on the seeded cells and incubated in a CO2 incubator. Aver the incubaWon period, 10 μL of MTT soluWon was added into each well and further incubated for 4 hours. The medium with excess MTT was removed from the wells, and 100 μL of dimethyl sulfoxide (DMSO) was added. The plate was shaken using a shaker at 300 rpm for 15 minutes. The result was read using spectral scanning mulWmode reader at 570nm wavelength. The enWre trial was set in triplicate and three independent experiments were done. The average of the triplicates from control and treatment wells was calculated. StaWsWcal analysis was carried out using SPSS version 26. A p value <0.05 was considered as staWsWcally significant. The cytotoxic effect of Tualang honey was expressed as IC50 value, where the concentraWon of Tualang honey was transformed in log10 and analysed with GraphPad Prism 9 sovware. Results: Cell viability greater than 65% was observed at doses of 0.16% to 5% Tualang honey. Cytotoxic effect of Tualang honey was at IC50 value of 5.478. Conclusions: Tualang honey can be considered as non-cytotoxic on HGFs at 5% concentraXon. Thus, at this percentage it is relaXvely safe for the growth of HGF cells. __________________________________________________________________________________________ 1 Periodontic Unit, Klinik Pergigian Jalan Sultan Mahmud, Kuala Terengganu, Terengganu, Malaysia 2 School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia Corresponding Author: Nor Azira binX Zahadi, PeriodonXc Unit, Klinik Pergigian Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia. E-mail: [email protected] Status of research: Completed
12 11. IMPACT OF NONSURGICAL PERIODONTAL THERAPY ON ORAL HEALTH RELATED QUALITY OF LIFE IN PRIMARY DENTAL CARE PATIENTS: A PROSPECTIVE COHORT STUDY Vanaja Planisamy1, Ee Ai Yin2 Aim: To determine the impact of nonsurgical periodontal therapy (NSPT) on Oral Health Related Quality of Life (OHRQoL) of a selected group of Malaysian populaWon at the primary dental care sejng. Materials and Methods: This study was conducted from year 2021-2022. PaWents aeending government dental clinics in Kinta district were included in the study aver basic periodontal examinaWon (BPE) was conducted. The sample size was calculated using the NCSS PASS 11 Power Analysis and Sample size calculator and a final sample size of 94 was used. A short version of the Malaysian Oral Health Impact Profile, S-OHIP(M) quesWonnaire was given as a baseline prior to the intervenWon. Nonsurgical periodontal therapy was provided in a single session or by quadrant approach. PaWents were then called for review aver 6 weeks during when they were required to complete the S-OHIP(M) quesWonnaire again. All data were entered and analysed using SPSS version 26.0. Results: The prevalence of impact decreased significantly post treatment [n (%) pre vs post: 91 (96.8%) vs 70 (74.5%); p<0.001]. A significant decrease was observed in the severity of impact (ADD score) [median (IQR) pre vs post: 8.5 (5.0, 15.0) vs 2.0 (0.0, 6.0); p< 0.001] and extend of impact (SC score) [median (IQR) pre vs post: 0.5 (0.0, 2.0) vs 0.0 (0.0, 0.0); p< 0.001] post treatment compared to pretreatment too. Conclusion: The OHRQoL status among gingiviWs and mild to moderate periodonWWs paWents in primary care has improved aver treatment. __________________________________________________________________________________________ 1 Klinik Pergigian Ipoh, Pejabat Kesihatan Pergigian Daerah Kinta 2 Klinik Pergigian Buntong, Pejabat Kesihatan Pergigian Daerah Kinta Corresponding Author: Vanaja A/P Planisamy, Klinik Pergigian Ipoh,Jalan Panglima Bukit Gantang Wahab,30590 Ipoh,Perak. Phone: 05-2456341. E-mail: [email protected] Status of research: Completed
13 12. CLINICAL AND METAGENOMIC INVESTIGATION OF LOCALLY DELIVERED SYNTHETIC ANTIMICROBIAL PEPTIDE IN NON-SURGICAL PERIODONTAL TREATMENT: A PILOT STUDY Tan Hooi Shan₁ , Mohd Zamri Bin Hussin2, Syarida Hasnur BinN Safii2, Syafinaz BinN Amin Nordin3 Background and aim: Nonsurgical periodontal treatment with subgingival instrumentaWon alone may not be as effecWve in some clinical situaWons. Thus, adjuncWve therapy should be considered to enhance periodontal treatment outcomes. This study aimed to evaluate the effect of a commercially available locally delivered syntheWc anWmicrobial pepWde (AMP) gel as an adjunct in nonsurgical periodontal treatment. Materials and Methods: Four stage III periodonWWs-affected subjects were recruited in a split-mouth study of 12-week follow-up. Aver full mouth professional mechanical plaque removal and subgingival instrumentaWon, all sites with probing pocket depth 6-7 mm were randomly assigned to test (AMP) or control (normal saline) group. Clinical examinaWon and subgingival plaque sampling were performed at baseline, 6 weeks, and 12 weeks. Clinical data was analysed with SPSS sovware. Subgingival plaque samples were analysed by 16S rRNA-gene sequencing, QIIME pipeline, and R sovware. Results: Clinical parameters were significantly improved in both groups at 6 weeks, and 12 weeks follow-up. There was no difference in PPD between groups, although greater reduction was observed in the test group. Adjunctive application of AMP demonstrated greater CAL gain (p <0.05) at 12 weeks as compared to control. Using the Shannon index, alpha diversity decreased after treatments and was significantly lower in the control group at 6 weeks. Principle coordinate analysis demonstrated a clear separation between pre-and post-treatment samples in the test group. At the genus level, a decrease in diseased-associated bacteria and an increase in health-associated bacteria were observed after both treatments. The genus Tannerella was significantly less abundant at 12 weeks compared to baseline in the test group. Conclusion: Local applicaWon of AMP gel in conjuncWon with subgingival instrumentaWon resulted in modest beneficial changes in clinical outcome and subgingival bacterial composiWon, supporWng its potenWal as an adjunct to nonsurgical periodontal therapy. __________________________________________________________________________________________ 1 Unit Pakar PeriodonXk, Klinik Pergigian Batu Pahat 2 Department of RestoraXve DenXstry, Faculty of DenXstry, UniversiX Malaya 3 Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Corresponding Author: Tan Hooi Shan, Unit Pakar Periodontik, Klinik Pergigian Batu Pahat. Phone: 012-5786363. E-mail: [email protected] Status of research: Presented as oral presentation in 14th Dental postgraduate research conference of Universiti Malaya via zoom on 22-23 September 2021
14 13. LONGITUDINAL CHANGES IN MASTICATORY FUNCTION FOLLOWING NONSURGICAL TREATMENT OF STAGE III AND IV PERIODONTITIS: A LONGITUDINAL OBSERVATIONAL STUDY Sand Ming Lee1, Chris Tat Cheun Fok2, Georgios Pelekos2, Wk Leung2, Maurizio ToneW3 Aim: To observe the changes in masWcatory funcWon along with oral health related quality of life of Stage III and Stage IV periodonWWs paWents aver Step 1 and Step 2 periodontal therapy. Material and Methods: A pilot longitudinal observaWonal study on 28 parWcipants, diagnosed with Stage III and IV periodonWWs and currently receiving periodontal therapy in IAD-MSC or Prince Phillip Dental Hospital. Subjects were selected via convenience sampling. MasWcatory efficiency was objecWvely measured by the variance of hue in a colour mixing chewing gum test using colorimetric sovware analysis, while subjecWve measurement was via visual categorizaWon. Oral health related quality of life was assessed via 2 quesWonnaires i.e., Oral Health Impact – 14 S (OHIP-14S) and CondiWon-Specific Oral Impact on Daily Performances for PeriodonWWs (CS-OIDP). StaWsWcal analysis was performed to test the differences on periodontal, masWcatory and oral health related quality of life parameters before and aver Step 1 and Step 2 therapy. Results: 16 Stage III and 12 Stage IV periodonWWs paWents completed the study. No staWsWcally significant changes were observed in changes in variance of hue and gum mixing subjecWve analysis aver Step 1 and Step 2 therapy. However, changes in the overall CS- OIDP and 3 OHIP-14S domain scores were posiWve and staWsWcally significant. This posiWve relaWonship was also found between the changes in total teeth with increased mobility and changes in variance of hue. Conclusions: Within the limitaWons of the study, masWcatory funcWon did not show significant improvement aver Step 1 and Step 2 periodontal therapy. However, certain aspects of quality of life did show significant improvement. No conclusion thus could be drawn on masWcatory funcWon improvement in Stage III and IV periodonWWs aver Step 1 and Step 2 periodontal therapy. __________________________________________________________________________________________ 1 Oral Health Division, Ministry of Health, Putrajaya, Malaysia 2 Faculty of Dentistry, University of Hong Kong, Hong Kong, China SAR 3 Shanghai PerioImplant Innovation Center, Department of Oral and Maxillo-facial Implantology, Shanghai Key Laboratory of Stomatology, National Clinical Research Centre for Oral Diseases, Shanghai Ninth People Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Corresponding Author: Lee Sand Ming. Unit PeriodonXk, Klinik Pergigian Luyang, Klinik Kesihatan Luyang, Off Jalan Lintas, 88300 Kota Kinabalu, Sabah. E-mail: [email protected] Status of research: Completed