Albumin Removal Kits

Albumin Removal Kits

Enrichment of Low Abundance Serum/Plasma Proteins

AlbuVoid™ AlbuSorb™

Selectively Voids Albumin, Binds Low Abundance Proteome Selectively Binds Albumin

 Albumin voids in flow- 2DE analysis of through,  Removes serum albumin
>95% AlbuVoid™ treated >90%
sheep serum. Samples
 <30 minute protocol were reduced, alkylated  Economical small ligand
and total protein surface architecture (not
 Low abundance normalized. The circled dye-based), bio-affinity
enrichment equivalent regions indicate the performance
or better than hexa- albumin zone. Gel 1:
peptides or antibodies Sheep serum sample.  Consumable, cost-effective,
Gel2: AlbuVoid™ no column regeneration or
 On-bead digestion Eluate. Gel 3: Same as cross-contamination
protocols, efficient 2 - AlbuVoid™ Eluate
LC-MS workflows but restained with  Species agnostic
SilverQuest
 Disposable, cost- (Invitrogen) silver  Compatible with
effective, no column stain. The differences o LC-MS
regeneration or between the gels o Microarrays
cross-contamination illustrate the efficiency o Functional assays
of albumin removal,
with no intrinsic pI or Non-reduced SDS-PAGE
MW bias. profiles

 Mild elution maintains Left lane: Normal human
native structure with pooled serum control
retained enzymatic,
functional & bio-activities Right lane:
Flow-through from
 Species agnostic AlbuSorb™

Product Size Item No. Typical Performance 100-200 µl Albumin Zone
AlbuVoid™ 5 Preps AVK-05 >95%*
AlbuVoid™ 10 Preps AVK-10 Serum Sample Volume 400-600
AlbuVoid™ 50 Preps AVK-50 Albumin Removal
3000-5000
LC-MS unique proteins >95%*
LC-MS unique peptides
Total Low Abundance Protein Recovery

Based on 100-200 µl serum preps * Estimates based on SDS-PAGE visualization combined with
Total Protein Assay.
Product Size Item No.

AlbuSorb™ 1 gm A185-1

AlbuVoid™ LC-MS On-Bead AlbuTrial™ Kit AlbuSorb™ 6 gm A185-6

1 gm processes 20 preps, 25 µl serum

 Seamless workflows samples

 Label, label free & glyco- compatible Don’t know which one to
try? Try both.
 Unique proteolytic efficiencies
4h O/N Product Item AlbuSorb™ PLUS
#
Total LC-MS/MS AlbuTrial™ Selectively Binds Albumin
Proteins identified at Kit AVS-05 & Immunoglobulin
2 digestion times: 4
hours (4h) & 114 217 154 The Kit includes: >400 µg total serum protein
overnight (O/N), 1 Gram AlbuSorb™ mass per prep (> 85% Albumin,
single 3 hour LC beads +
gradient. 5 Preps AlbuVoid™

>85% IgG depleted) from 25 µl

Human Serum: Total 485 serum

Product # Item No.

Product Size Item No. Preps
AlbuVoid™ 5 Preps AVB-MS05
LC-MS On-Bead AlbuSorb™ 20 APK285-

PLUS 20

AlbuVoid™ 10 Preps AVB-MS10 AlbuSorb™ 100 APK285-
LC-MS On-Bead
PLUS 100

Based on 50-100 µl serum preps

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