E BOOK CSI102

E BOOK CSI 102

TISSUE
MICROTOMY

SHARIFAH AWFA BINTI
SYED ALI (2022487028)

(P2HS1111A1)
&

NURUL IZZATI BINTI MOHD
NOOR (2022623654)
(P2HS1111A1)

INTRODUCTION

DEFINITION :

Microtomy or se
ction cutting is
the technique of making the
very thin slices of tissue

specimens for the microscopic
examination to identify the
abnormalities or atypical
appearance in the tissue (if

present) and also for the study
of various components of the

cells or tissues.

IMPORTANCE

bridges the process
between specimen

collection and
microscopic analysis.

Microtomes will
advance an object for

a predetermined
distance then slide the
object to the cutting

tool.

PRINCIPLES

The rotary mirotome
uses a staged rotary
action where the
specimen is cut with
the circular motion of
the handwheel to
facilitate the studies
under the microscope.
The cutting procedure
is done with a
disposable blade within
the instrument.

PART OF
ROTARY
MICROTOME

PROCEDURES

STEP 1

WIND BACK THE COARSE

FEEDING
MECHANISM AND

LOCK THE HAND WHEEL

STEP 2

CLAMP CASSETTE IN POSITION ,
ORIENTATING THE TISSUE AS

DESIRED

STEP 3

ENSURING THE KNIFE HOLDER
IS A SAFE DISTANCE FROM THE

CASSETTE , CAREFULLY
PLACE A BLADE INTO

THE HOLDER AND
TIGHTEN SECURELY

STEP 4

LOOSEN BACK LEVER OF KNIFE
HOLDER AND ADVANCE TOWARDS
THE CASSETTE UNTIL THERE IS A
0.2 - 0.5 MM INTERVAL BETWEEN
BLADE EDGE AND CASETTE SURFACE.






STEP 5
TIGHTEN BACK THE

LEVER OF KNIFE HOLDER

STEP 6

UNLOCK WHEEL AND
TRIM AWAY SURPLUS

WAX FROM
TISSUE SURFACE BY ADVANCING THE

COARSE
FEED MECHANISM BEFORE
COMMENCING EACH TURN OF THE WHEEL

STEP 7

TRIM ALL TISSUE CAREFULLY
UNTIL THE DESIRED SURFACE

IS EXPOSED.

STEP 8

REDUCE SECTION
THCIKNESS

TO 3-5 MICRONS
USING ADJUSTMENT KNOB.

STEP 9

CUT TISSUE SECTIONS
AND PLACE THEM ON TISSUE

FLOATATION BATH TO
REMOVE WAX.





Straight • Block face uneven ; • Block holder
ribbon one edge is coarse must be
difficult to faced before the rest aligned

cut of the block.

Ribbons • Tissue block is too • Block must be
compress & warm. chilled well.
• Use
crumple • Slow & uneven consistent
rotations increase wheel rotation
to get even
compression. ribboning.
• Paraffin is too sticky;
• Need paraffin
need to be harder. with higher

melting point.

Smooth • Dull blade. • Change to a
ribbons do • Knife clearance is new blade.
not form
too small. • Increase knife
clearance.

Large holes, • Tissue not • Re-embed
missing tissue embedded flat; tissue.
will not show
during sectioning

Parts of tissue • Tissue not • Re-processing
not showing on processed tissue as
properly and will required.
slides not form a

section.

ADVANTAGES
AND

DISADVANTAGES

ADVANTAGE DISADVANTAGE

By having more The high price
weight, it has due to the
more precision,
complexity of the
it allows advance
obtaining very
thin serrated mechanism, which
also makes
sections.
repairs more
difficult and

expensive.

The advance The impossibility
mechanism is of cutting with it
more accurate. tissues included

in celloidin, in
gelatin and in
propylene glycol.

PRECAUTIONS
WHEN USING
MICROTOME

Placement of the blade

Always set the rotary mirotome in locked
positions when changing a blade.
Place a new blade in the blade holder &
clamp it before the rotary wheel lock is
released.

Removal of blade

Disposable blade must be removed
using forceps & placed directly into a
sharp container.
Always wear gloves when remove a
blade.

Cleaning of the mirotome

The rotary must be locked & blade is
removed from the holder before cleaning.
Use a disinfectant that is effective against
infectious agents.