BIOLOGY PRE LAB QUESTIONS

DB014 Pre-Lab Module

EXPERIMENT 1: BASIC TECHNIQUE IN MICROSCOPY

Course Learning Outcome:
Solve problems related to cells, biomolecules, inheritance, genetics and biological development.
(C4, PLO 4, CTPS3, MQF LOD 6)

Learning Outcomes:
At the end of this lesson, students should be able to:
i. Identify parts and functions of light compound microscope
ii. Describe characteristics of image observed by microscope
iii. Calculate actual magnification

Student Learning Time:

Face-to-face Non face-to-face

1 hour 1 hour

Direction: Read over the lab manual and then answer the following questions.

Introduction

1. What is light compound microscope?
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2. How do we hold the microscope properly?
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3. When do we use the oil immersion objective lens?
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4. What is the highest magnification should you focus before using the oil immersion
objective lens?
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DB014 Pre-Lab Module
5. Based on FIGURE 1, identify the components of the light compound microscope.

FIGURE 1

FIGURE 1

Label Name Function
A
C
D
E
G
I
J
K
L
M

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DB014 Pre-Lab Module

L

‘L’ seen by naked eye Inverted letter ‘L’

FIGURE 2

1. Draw the orientation of ‘L’ observed by the light compound microscope in
FIGURE 2.

2. Based on FIGURE 3, state the relationship between magnification and the depth of
field.
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FIGURE 3 3
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DB014 Pre-Lab Module

3. Based on FIGURE 4, state the relationship between magnification and the field of
view.
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FIGURE 4

4. Actual Magnification = Magnification power x Magnification power

of objective lens of ocular lens

The above formula is used to determine the actual magnification of a specimen. You
are given a specimen of Escherichia coli to observe its shape and colour. Calculate
the actual magnification to observe the specimen?
(* Assume the magnification of the ocular lens is 10x)

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Updated: 03/04/2019 4

DB014 Pre-Lab Module

EXPERIMENT 2: ANIMAL TISSUE

Course Learning Outcome:
Solve problems related to cells, biomolecules, inheritance, genetics and biological
development.
(C3, PLO 4, CTPS3, MQF LOD 6)

Learning Outcomes:
At the end of this lesson, students should be able to:
i. Identify the major types of animal tissues.
ii. Identify the structure of epithelial tissue, muscle tissue and connective tissue.

Student Learning Time:

Face-to-face Non face-to-face

1 hour 1 hour

Direction: Read over the lab manual and then answer the following questions.

Introduction

1. Define tissue.
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2. How are tissues related to organs?
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3. The four basic types of animal tissues are epithelial tissue, muscle tissue, connective
tissue and nerve tissue. State the unique characteristics of epithelial tissue.
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DB014 Pre-Lab Module
4. FIGURE 1 shows three different types of muscle tissue. Name the tissues A, B and C.

Tissue A

Tissue B

Tissue C

FIGURE 1
Tissue A : ______________________________________________________________
Tissue B : ______________________________________________________________
Tissue C : ______________________________________________________________

Experiment
1. Give the structural differences between simple squamous epithelium, simple cuboidal

epithelium and simple columnar epithelium.
Simple squamous epitheliSuimmple cuboidal epithelium Simple columnar
epithelium

Cell shape
Shape of nucleus

2. What is the functional unit of nervous system?
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DB014 Pre-Lab Module
3. FIGURE 2 shows muscle M found in the stomach. Name muscle M.

Tissue M

FIGURE 2

Muscle M : ________________________________________________________

4. FIGURE 3 shows blood tissue. Identify D, E and F.

D
E

F

FIGURE 3
D : ______________________________________________________________
E : ______________________________________________________________
F : ______________________________________________________________

DB014 Pre-Lab Module

EXPERIMENT 3: PLANT TISSUE

Course Learning Outcome:
Solve problems related to cells, biomolecules, inheritance, genetics and biological development.
(P3, CLO 2, PLO2, MQF LOD 2)

Learning Outcomes:

At the end of this lesson, students should be able to:

i. To identify the plant tissue under the light compound miscroscope

ii. To identify different types of ground and vascular tissue

Student Learning Time:

Face-to-face Non face-to-face

1 hour 1 hour

Direction: Read over the lab manual and then answer the following question.

Introduction

1. State THREE main characteristics of plant cell.
________________________________________________________________________

________________________________________________________________________

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2. Plant tissues are divided into two groups which are meristem and permanent tissue. State
the main difference between meristem and permanent tissues.
________________________________________________________________________

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3. FIGURE 1 shows a dicotyledonous plant. Identify the permanent and meristem tissues.

FIGURE 1

DB014 Pre-Lab Module
4. Based on FIGURE 2, identify the ground tissues and vascular tissues with their

corresponding distribution in plant.

FIGURE 2
5. State the main tissues of vascular bundle in plant.

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Experiment
1. a) What is the purpose of using iodine in this experiment?

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b) Why onion is used in this experiment?
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2. List TWO precautions of the experiments.
i. ______________________________________________________________________
ii. ______________________________________________________________________

DB014 Pre-Lab Module

EXPERIMENT 4: CELL DIVISION- MITOSIS

Course learning outcome:
Conduct basic biology laboratory work on microscopy, tissues, genetics information, pollen
germination and plant growth by applying manipulative skills.
(P3, CLO 2, PLO 2, MQF LOD 2)

Learning outcomes :
At the end of this lesson, students will be able to:
(i) Identify stages in mitosis

Student Learning Time:

Face-to-face Non face-face-face

1 hour 1 hour

Direction: Read over the lab manual and then answer the following questions.

Introduction

1. What is mitosis?

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2. (a) What kind of cell go through mitosis?

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(b) State ONE location of the tissue in 2(a) above.

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3. State ONE importance of cell division

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4. List the FOUR stages of mitosis

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DB014 Pre-Lab Module

Experiment
1. Name the cell structure that might be able to observe under light microscope.

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2. How to determine the stages in mitosis under microscope?

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3. The following cells is in which of mitosis stages? Give your reason based on the

chromosomal behavior.

Stage : ___________ Stage : ___________ Stage : _________ Stage : _________

Reason : Reason : Reason : Reason :

___________________ _________________ _______________ _______________

___________________ _________________ _______________ _______________

4. Answer the following questions based on the diagram
(i) Name the stage.
(ii) State the current number of chromosome.
(iii)Predict the number of chromosome at the end of mitosis.

(i) _______________ (i) _______________
(ii) _______________ (ii) _______________
(iii)_______________ (iii)_______________

_ _

DB014 Pre-Lab Module

EXPERIMENT 5: POLLEN GERMINATION

Course Learning Outcome:
Solve problems related to cells, biomolecules, inheritance, genetics and biological
development.
(C3, PLO 4, CTPS3, MQF LOD 6)

Learning Outcomes:
At the end of this lesson, students should be able to:
i. Define double fertilization.
ii. State reproductive organs in plant.
iii. Sketches and label a parts of flower.
iv. Explain the function of generative nucleus and pollen tube nucleus.

Student Learning Time: Non face-to-face
Face-to-face 1 hour
1 hour

Direction: Read over the lab manual and then answer the following questions.

Introduction

1. Define double fertilization
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2. (a) List the female reproductive organ in plant.
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(b) List the male reproductive organ in plant.
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3. Sketches and label a parts of flower.

4.

FIGURE 1 below shows the mature pollen grain in a flower.

DB014 Pre-Lab Module

C
A

B

FIGURE 1
(a) Based on FIGURE 1, label a parts of one mature Pollen Grain:

(i) A: _______________________________
(ii) B: ________________________________
(iii) C: ________________________________

(b) Based on FIGURE 1, explain the function of generative nucleus and pollen
tube nucleus.
(i) Generative nucleus:
_______________________________________________________________
_______________________________________________________________
(ii) Pollen tube nucleus
_______________________________________________________________
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DB014 Pre-Lab Module

EXPERIMENT 6: PLANT GROWTH
Course Learning Objective: Conduct basic biology laboratory work on microscopy, tissues,
genetics information, pollen germination and plant growth by applying manipulative skills.
(P3, CLO 2, PLO 2, MQF LOD 2)

Learning Outcomes:

At the end of this lesson, students should be able to:

i. To observe and record the growth of a bean sprout.
ii. To measure growth parameter (height of plant, length of root and length of leaf).

Student Learning Time (SLT):

Face-to-face Non face-to-face

2 hour 0

Direction: Read over the lab manual and then answer the following questions.

Introduction

1. What is growth?

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2. Name the phases of growth

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3. What is the growth parameter used to measure the growth of bean sprout?

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4. State the growth pattern for most of the organism.
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