EXPERIMENT 3

BIOLOGY DB014

EXPERIMENT
3

PLANT TISSUES

DB014 Lab Manual

EXPERIMENT 3: PLANT TISSUES

Course Learning Objective: Conduct basic biology laboratory work on
microscopy, tissues, genetics information, pollen germination and plant
growth by applying manipulative skills.
(P3, CLO 2, PLO 2, MQF LOD 2)

Learning Outcomes:
At the end of this lesson, students should be able to:
i. Identify the plant tissue under the light compound microscope
ii. Identify different types of ground and vascular tissue.

Student Learning Time (SLT):

Face-to-face Non face-to-face

2 hours 0

Introduction

Permanent tissues consist of mature cells that have specialized
structure and functions. Permanent tissues can be divided into three:
dermal tissues (epidermal and peridermal), ground tissues
(parenchyma, sclerenchyma, collenchyma and endodermis) and
vascular tissues (xylem and phloem).

Apparatus

Compound microscope

Materials

Prepared slides of cross sections of monocot/dicot stem and root

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Experiment 3.1: Structures of plant tissues in plants

1. Examine the prepared slides of any types of plant tissues
(root/ stem)

2. Identify the distribution of the tissues (parenchyma,
collenchyma, sclerenchyma, phloem and xylem).

3. Draw the arrangement of the tissues. Note the differences in the
following characteristics: cell size, shape, wall thickness and
stained parts.

4. Select a section and observe it under 40x objective lens. Draw
and label the section showing the different tissues (parenchyma,
collenchyma, sclerenchyma, phloem and xylem). Use the figures
provided to assist you in your tissue investigation.

Figure 3.1 Structure and distribution of vascular bundles
incross section of monocot root. (Adapted from
www.sci.waikato.ac.nz)

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Figure 3.2 Cross section of monocot root

Figure 3.3 Structure and distribution of vascular bundles in
cross section of dicot root

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Figure 3.4 Cross section of dicot root.
(Adapted from www.sci.waikato.ac.nz)

Figure 3.5 Structure and distribution of vascular bundles
in cross section of monocot stem.

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Figure 3.6 Structure and distribution of vascular bundles in
cross section of dicot stem.
(Adapted from www.bio.miami.edu)

Monocot Dicot

Figure 3.7 Structure of different plant tissues in cross
section of monocot and dicot stem

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Questions:

1. Describe the types, functions and distributions of tissues below:

a) Ground Tissues
- Parenchyma cells
- Collenchyma cells
- Sclerenchyma cells

b) Vascular Tissues
- Xylem tissue
- Phloem tissue

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