THE Autoradiography - GE Healthcare Life Sciences

THE

Autoradiography

PRODUC T GUIDE

Hyperfilm

Kodak Film

Autoradiography
Accessories

18115258

Introduction

Amersham Biosciences is one of the world’s leading suppliers of
autoradiography and light detection products. Using its expertise in signal
generation, Amersham Biosciences has developed Hyperfilm™ and
accessory products specifically for use in life science applications, providing
quality results and capturing the maximum amount of information.
As well as our own well-established autoradiography range, the company is also
a worldwide distributor of Kodak™ Scientific Imaging Systems’ autoradiography
products. Amersham Biosciences is therefore able to advise customers
on the best solution for their imaging needs.
In addition to this catalogue, Review No.23, Efficient Detection of
Bio-molecules by Autoradiography, Fluorography or Chemiluminesence,
by R. Laskey, is also available. Review 23 is an independently written reference,
that describes in detail, the principles of indirect and direct autoradiography.
Review 23 is available on request, please contact your
local sales office. Alternatively you can download a
copy from the Web here.

1.

Contents 3–4

Life science application guide 5–6
7–8
Autoradiography films: 9 – 10
Hyperfilm MP
Hyperfilm ECL 11
Kodak BioMax MR 12
Kodak BioMax MS 13
Kodak BioMax Light 13
Kodak X-Omat AR (XAR)
Kodak X-Omat LS (XLS) 14
15
Autoradiography cassettes and screens: 16
Hyperscreen Intensifying Screens 17
Kodak BioMax MS Intensifying Screens
Kodak BioMax TranScreen Intensifying Screens 18
Hypercassette Autoradiography Cassettes 19
19
Autoradiography accessories: 20
Amplify Fluorographic Reagent 21
Darkroom Lamp 22
Hyper torch 23 – 24
ECL Mini-Camera 25
Hypercoat Nuclear Emulsions 26
Hyperprocessor Automatic Film Processor 27
Liquid Scintillation Cocktails 27
Microscale Autoradiography Standards
Sensitize Pre-flash Unit 28
Processing Hanger 29
TrackerTape

Other film products:
Kodak Duplicating Film
Kodak Micro-Autoradiography & TEM products

2.

Life science APPLICATION GUIDE

Autoradiography methods and materials for life science applications

Application Result required Label

Southern/Northern blots high speed and sensitivity 32P
Plaque/colony lifts accurate quantitation
maximum speed and sensitivity 32P
Slot blots optimum resolution 33P/35S
non-radioactive, quantification ECL™, Gene Images™
Dideoxy sequencing high speed and sensitivity 32P
accurate quantitation
Cycle sequencing maximum speed and sensitivity 32P
Protein synthesis, lysates non-radioactive, quantification ECL, Gene Images
Western blots high speed 32P
optimum resolution and maximum 35S
In situ hybridization speed for difficult sequences
Receptor studies good resolution and speed for 35S
routine work
Whole body autoradiography optimum resolution and maximum 33P
speed for difficult sequences
good resolution and speed for 33P
routine work
maximum speed 35S/14C/3H
optimum resolution 125I
maximum speed and sensitivity 125I
accurate quantification
optimum resolution 35S/14C
non-radioactive/maximum sensitivity ECL
macroscale in situ optimization 32P
of parameters
localization at cellular level: 33P/35S
– intermediate resolution 33P/35S
– high resolution
localization subcellular level: 125I/3H
– high resolution 35S/14C
high resolution

3.

Method Film Support products
Sensitize™, Hyperscreen™
Pre-flash and screens at -70ºC Hyperfilm MP BioMax TranScreen HE
Sensitize
Screens at -70ºC BioMax™ MS Sensitize, Hyperscreen
Direct autoradiography BioMax MR BioMax TranScreen HE
Pre-flash and direct detection Hyperfilm ECL Sensitize
Pre-flash and screens at -70ºC Hyperfilm MP
Amplify, Sensitize
Screens at -70ºC BioMax MS BioMax TranScreen HE
Pre-flash and direct detection Hyperfilm ECL Sensitize, Hyperscreen
Direct autoradiography Hyperfilm MP/BioMax MR BioMax TranScreen HE
Direct autoradiography BioMax MR Sensitize

Direct autoradiography Hyperfilm MP

Direct autoradiography BioMax MR

Direct autoradiography Hyperfilm MP

Pre-flash and fluorography at -70ºC Hyperfilm MP
Indirect autoradiography BioMax MS
Pre-flash and screens at -70ºC Hyperfilm MP
Screens at -70ºC BioMax MS
Direct autoradiography BioMax MR
Pre-flash and direct detection Hyperfilm ECL
Direct autoradiography Hyperfilm MP

Direct autoradiogaphy BioMax MR
Micro autoradiography Hypercoat™ emulsion LM-1

Micro autoradiography Hypercoat emulsion EM-1
Direct autoradiography BioMax MR

4.

NEW FORMULATION Hyperfilm MP

Product description In a multi-discipline laboratory, performing both blots and
sequencing experiments, new formulation Hyperfilm
The new, higher performance photographic emulsion MP is the recommended film for high performance in all
utilized in new formulation Hyperfilm MP not only applications.
offers up to 15% greater sensitivity over original
Hyperfilm MP, but also improved contrast and resolution. New formulation Hyperfilm MP is also available in an
envelope sealed format. Here, individual sheets of Hyperfilm
Like its predecessor, new formulation Hyperfilm MP MP are sealed in light tight envelopes giving maximum
is an excellent multipurpose film constructed from protection and ease of use in the life science laboratory.
a clear base coated on both sides with a photographic Each envelope is made from paper with an internal lining
emulsion that is then protected by an anti-scratch layer. of low-density polymer. A single sheet of paper interleaving
It may be processed using automatic processors or in between the film and envelope prevents static.
tanks and trays using most common laboratory X-ray
film developers and fixers.

anti- screen 1 Volume (AU) 3000
scratch
layer emulsion 2000
plastic base New Formulation
emulsion Hyperfilm

32P/125I 1000

radioactive emissions Current Formulation
0
light emission
1 3 5 10
FIG 1 pg of target DNA
Diagram of Hyperfilm MP being used with an intensifying screen
FIG 2
Measure made on Personal Densitometer™ SI, analysis using
ImageQuant™. λ Hind III dots hybridized at 65°C in Church Buffer
using 32P Megaprime™ labelled probe at approx 1 ng/ml in
hybridization buffer. Exposure time 24hrs at -70°C with
Hyperscreen™

5.

Applications Features and benefits

q 32P, 33P and 125I Northern, Southern and q New formulation film up to 15% more sensitive
Western blots than traditional Hyperfilm MP when utilised with
an intensifying screen
q 35S, 33P and 32P sequencing
q Fluorography of 3H, 14C and 35S q Coated with emulsion on both sides to ensure
optimum sensitivity

q Multipurpose film for the high speed detection
of 3H, 14C, 35S, 33P, 32P and 125I

q Clear base offers high contrast for easy reading
of images

q High resolution results at an economical price

q Designed for use in direct and indirect autoradio-
graphy, the film of choice for multipurpose work

A New Hyperfilm MP B Old formulation Hyperfilm MP FIG 3
Single copy gene detection using 32P. Southern blots
of human placental DNA Hind III digest (1, 0.5,
0.25 µg per lane). Detection with N-ras insert probe
(1.5kb) labelled with 32P-dCTP. Autoradiography at
–70°C with Hyperscreen™ for 36 hours.

Ordering information Code Size Quantity

Product RPN6K 18x24cm 25 sheets
Hyperfilm MP RPN1675K 18x24cm 75 sheets
RPN36K 18x43cm 25 sheets
Hyperfilm MP envelope RPN2115K 24x30cm 25 sheets
RPN7K 30x40cm 25 sheets
RPN8K 35x43cm 25 sheets
RPN30K 35x43cm 75 sheets
RPN1676K 5x7in 25 sheets
RPN1677K 8x10in 25 sheets
RPN1678K 8x10in 75 sheets
RPN34K 20cmx25m 1 roll

RPN6L 18x24cm 50 sheets
RPN8L 35x43cm 50 sheets
RPN1677L 8x10in 50 sheets

6.

NEW FORMULATION Hyperfilm ECL

Product description 2.0

The new, high performance emulsion in new Film CD 1.5
formulation Hyperfilm ECL delivers up to a 30%
improvement in sensitivity over traditional Hyperfilm New Film
ECL with both blue and green light chemiluminescent 1.0
systems. The emulsion of this double-coated film
provides improved clarity and sensitivity for the 0.5
detection of chemiluminescent-labelled samples, Old Film
making it suitable for ECL, ECL Plus™, CDP-Star™
and all other chemiluminescent systems. 0.0 100 1000 10000
10 Relative Blue LIght Emittance (Log Scale)
Applications
FIG 4
q Chemiluminescent Northern, Southern and Comparison of luminescent response between new
Western blots and old formulation Hyperfilm ECL

A New formulation Hyperfilm ECL B Old formulation Hyperfilm ECL

FIG 5
Western blots of post synaptic density preparation detected on new and old formulation Hyperfilm ECL for 30 second exposures.
Primary antibody 1/2000 mouse anti-synapsin 1a/1b monoclonal, secondary antibody 1/1000 rabbit anti-mouse.

Results supplied by Catherine Scott. Research School of Biosciences, University of Kent at Canterbury

7.

Features and benefits q Can be processed in automatic processors or
manually using most common X-ray film developers
q New formulation film up to 30% more sensitive and fixers
than traditional Hyperfilm ECL
q Single coated emulsion, making it the film of choice
q High speed detection of chemiluminescence signal for maximum resolution
from nucleic acid and protein blots
q Clear base offers high contrast against blackened
q System tested with ECL and Gene Images kits image for easy reading and scanning
to ensure optimum performance

q Clear base offers high contrast for easy reading
of images

Ordering information

Product Code Size Quantity
Hyperfilm ECL RPN2103K 18x24cm 25 sheets
RPN3103K 18x24cm 75 sheets
RPN2104K 30x40cm 25 sheets
RPN1674K 5x7in 25 sheets
RPN2114K 8x10in 25 sheets
RPN3114K 8x10in 75 sheets
RPN1681K 10x12in 25 sheets

8.

KODAK BioMax MR

Product description background clarity, making it the ideal choice for
sequencing. It can be processed manually or in an
Kodak BioMax MR (maximum resolution) film, automatic processor.
is a single emulsion film that utilizes T-Grain technology.
The single emulsion format increases resolution and

Standard emulsion

35S Sample

Globular silver halide crystals cover less surface area Tabular grain silver halide crystals are flat, covering more surface area increasing number of interactions
Large mass of crystals stop 35S beta particles, reducing efficiency per particle in exposing crystals between beta particles and crystals

FIG 6 Because crystals are flat each beta particle can expose multiple crystals
BioMax emulsion increases sensitivity to 35S, 33P and 14C

FIG 7 Applications
Thermo Sequenase™
cycle sequencing q 35S and 33P sequencing
using Redivue™
Nucleotides (33P).
2 hour exposure
on Kodak BioMax
MR film

9.

Features and benefits q Single coated emulsion, making it the film of choice
for maximum resolution
q High speed direct autoradiography of samples labelled
with 14C, 35S and 33P q Clear base offers high contrast against blackened
image for easy reading and scanning
q T-Grain emulsion gives two-fold improvement in
sensitivity over Kodak X-Omat™ AR film for direct
exposure of 14C, 35S and 33P

Ordering information

BioMax MR-1 Film (non-interleaved packaging)

Code Size Quantity
V8701302 8x10in 50 sheets
V1314319 5x7in 25 sheets
V8715187 35x43cm 50 sheets
V8736936 18x24cm 50 sheets
V8756447 18x43cm 50 sheets
V8912560 24x30cm 50 sheets
V8929655 30x40cm 50 sheets
V8809360 33x61cm 50 sheets

BioMax MR-2 Film (Ready Pack – each sheet individually wrapped) Quantity
50 sheets
Code Size 25 sheets
V8952855 8x10in 50 sheets
V8202160 5x7in
V8567232 35x43cm

10 .

KODAK BioMax MS

Product description Applications

Kodak BioMax MS (maximum sensitivity) is a double q 32P Northern and Southern blots
sided film that utilizes Kodak T-Grain emulsion technology
to maximise interaction with beta particles (see Fig 6, Features and benefits
page 9) and is designed for maximum performance with
32P and 125I. When used with the BioMax MS or BioMax q State of the art technology, the film of choice for
TranScreen™ HE intensifying screens to detect 32P, maximum sensitivity
BioMax MS film forms one of the fastest conventional
autoradiography film and screen systems available.The q Reduces 4-day exposures to 1-day and overnight
emulsion is extremely light sensitive, in particular to the exposures to 4 hours
green light emissions of BioMax intensifying screens.
Note:This film should only be exposed to a red Kodak q Detects 1 dpm bands in 1 day
GBX-2 safelight filter using a 7.5 or 15 watt bulb.
It can be processed manually or in an automatic processor. q Can be processed in automatic processors or manually
(using most common X-ray film developers and fixers)

4.0

Transcreen Generic screen/ Net optical density (total density – gross fog) 3.5
HE screen/ X-Omat AR film 3.0 BioMax MS film/MS screen
BioMax MS film

13.8 2.5

6.9 2.0
XAR film/screen
BioMax Light and
1.5 XLS film/screen

3.4 1.0

0.5

1.7 0.0
-0.3 0
0.3 0.6 0.9 1.2 1.5 1.8 2.1 2.4 2.7 3.0 3.3

0.86 Relative log activity, 32P (dpm), 40 hour exposure

dpm/band FIG 8 FIG 9
100-hour exposure at -70ºC Response to 32P Comparison of film sensitivity to 32P

Ordering information

BioMax MS-1 Film (non-interleaved packaging)

Code Size Quantity
V8294985 8x10in 50 sheets
25 sheets
V1179258 5x7in 50 sheets
50 sheets
V1435726 35x43cm 50 sheets
V8222648 18x24cm 50 sheets
V8326886 24x30cm

V8220774 30x40cm

BioMax MS-2 Film (Ready Pack – each sheet individually wrapped)

Code Size Quantity
V8377616 8x10in 50 sheets
V1473941 5x7in 25 sheets
50 sheets
V1764505 35x43cm

11.

KODAK BioMax Light

Product description Features and benefits

A double coated emulsion film from Kodak for the Hyperscreen
detection of chemiluminescence.
q Suitable for the detection of chemiluminescence
Applications
q Can be processed in automatic processors or
q Chemiluminescent blots manually using most common X-ray film developers
and fixers

64 pg 64 pg 64 pg

32 pg 32 pg 32 pg

16 pg 16 pg 16 pg

8 pg 8 pg 8 pg

4 pg 4 pg 4 pg

A BC

FIG 10
Comparison of BioMax Film exposure to chemiluminescence.
Nucleic acid probe, Human β-Actin cDNA, exposure time 5 minutes.
A Kodak BioMax Light B Kodak X-Omat AR C Kodak BioMax MS

Ordering information Size Quantity
8x10in 50 sheets
BioMax Light-1 Film 5x7in 25 sheets
(non-interleaved packaging) 35x43cm 50 sheets
18x24cm 50 sheets
Code 24x30cm 50 sheets
V1788207 30x40cm Discontinued.xAlternativexproductxRPN2104K.
V8442907
V8625170 Quantity
V8194540 50 sheets
V1538966 25 sheets
V8122004

BioMax Light-2 Film
(Ready Pack – each sheet individually wrapped)

Code Size
V8761520 8x10in
V1139435 5x7in

12 .

X-OmatKODAK AR (XAR)

Product description Applications

Kodak X-Omat™ (XAR) is a general-purpose film for q Southern, Northern and Western blotting
use with all commonly used isotopes. Coated with
emulsion on both sides, it provides high sensitivity for Features and benefits
both direct exposure autoradiography and exposures
with the Hyperscreen™, BioMax MS or TranScreen q A popular general-purpose film, compatible with
Intensifying Screens. 35S, 14C, 33P, 32P and 125I

Ordering information Quantity
50 sheets
X-Omat AR-5 Film (alternate interleaved packaging) 25 sheets
50 sheets
Code Size 50 sheets
V1651454 8x10in 50 sheets
50 sheets
V8447195 5x7in 1 roll
1 roll
V1651512 35x43cm

V8532665 18x24cm

V1501451 24x30cm

V1506955 30x40cm

V1531680 20cmx25m

V1510478 14inx50ft x

X-Omat AR-2 Film (Ready Pack – each sheet individually wrapped)

Code Size Quantity
V1651579 8x10in 50 sheets
25 sheets
V1036037 5x7in 50 sheets

V1651678 35x43cm

X-OmatKODAK LS (XLS)

Product description Applications

Kodak X-Omat LS (XLS) is an economical, blue tinted q Southern and Western blotting, and sequences giving
film for use with all commonly used isotopes. It provides strong signals
significantly lower detection sensitivity than BioMax or
XAR films resulting in longer exposure times. Features and benefits

q Reduces costs where sensitivity is not required

q Similar performance to other ‘blue’ tinted films

Ordering information

X-Omat LS-1 Film (non-interleaved packaging)

Code Size Quantity
50 sheets
V8646770 8x10in (20.3x25.4cm) 50 sheets
25 sheets
V8926024 35x43cm (14x17in) 50 sheets

V1036227 5x7in (13x18cm)

13 . V8688681 18x24cm (7x9.5in)

Hyperscreen INTENSIFYING SCREENS

Product description Features and benefits

Hyperscreen Intensifying Screens are economical high Hyperscreen
performance calcium tungstate screens for use with 32P q For rapid results and high sensitivity when detecting
and 125I.They can be used with pre-flashed film to give an
increase in sensitivity while retaining a linear response that 32P and 125I, in Southern, Northern and Western blots
allows quantitation of results.
q Light emission from calcium tungstate phosphor,
Applications fully compatible with Hyperfilm MP

q Southern, Northern and Western blots using 32P and 125I q Use with Sensitize Pre-Flash Unit for even higher
sensitivity and accurate quantification
– + ++
q Fine grain screens give clear band resolution
– minus
+ plus Hyperscreen q Available in a full range of sizes to match
++ plus Hyperscreen Hypercassette and Hyperfilm MP

and pre-flashed Kodak Intensifying Screen Cleaner
with Sensitize q Specially formulated solution for dust and dirt

removal and static reduction, recommended for all
Kodak and Hyperscreen Intensifying Screens

FIG 11 FIG 12
125I-labelled IgG slot Intensifying Screen
blots – doubling dilutions Cleaner and
(100nCi to 1.56nCi) Antistatic Solution
2 hour exposure to
Hyperfilm MP Quantity
1 pair
Ordering information Size 1 pair
18x24cm 1 pair
Hyperscreen 24x30cm 1 pair
30x40cm 1 pair
Code 35x43cm 1 pair
RPN1662 18x43cm 1 pair
RPN1663 20x40cm 1 pair
RPN1664 5x7in 1 pair
RPN1665 8x10in
RPN1666 10x12in Quantity
RPN1667 1 bottle
RPN1668
RPN1669
RPN1670

Kodak Intensifying Screen Cleaner and Antistatic Solution

Code Size 14 .
V1064930 16oz

KODAK BIOMAX MS

Intensifying Screens

Product description Features and benefits

Kodak BioMax MS Intensifying Screens are special q Doubles the speed of conventional film
screens that can be used with any autoradiography film
to increase sensitivity. Maximum sensitivity is achieved q Uses conventional intensifying screen format
when used with BioMax MS film.
q When used with BioMax MS Film it will increase speed
Applications of detection four fold (compared to normal film
screen combinations)
q Southern, Northern and Western blots using 32P and 125I
X-Omat AR BioMax MS
film/screen film/screen

BioMax MR Film BioMax MS Film and MS Screen
35S 33P
3H 14C 32P 32P & 125I

Sample

Overcoat 7 mil Emulsion FIG 14
(Includes Estar Base Layer BioMax film and
Protective Dye) intensifying screen:
Emulsion maximum speed
Layer for 32P blotting
applications
Intensifying
Screen

FIG 13 Before – NOW –
Principle of direct autoradiography and normal intensifying screens 72 hours 16 hours

32P labelled slot blots exposed at -70°C

Ordering information

Kodak BioMax MS Intensifying Screen

Code Size Quantity
V8142358 5x7in 1 screen
V8518706 8x10in 1 screen
V8757270 18x24cm 1 screen
V8981706 35x43cm 1 screen
V8967754 24x30cm 1 screen
V8770331 30x40cm 1 screen

BioMax MS-1 Film and Screen Combination Pack (non-interleaved, 50 sheets per box with BioMax MS Intensifying Screen)

Code Size Quantity
V1165893 8x10in 1 each
V1188077 35x43cm 1 each

15 .

KO DAK BIOMAX

TranScreen INTENSIFYING SCREENS

Product description Features and benefits

BioMax TranScreen Intensifying Screens incorporate a TranScreen LE
new, innovative technology and unlike conventional
screens they capture the beta particles prior to their q When used with BioMax MS Film it allows images of
being exposed to the film.The BioMax TranScreen then 35S, 14C, 33P, 45Ca, 59Fe and even 3H to be detected at
converts the energy from the beta particles to photons least 5 times faster than conventional film screen systems
without attenuation and directs the photons to the film.
q Offers two-fold better resolution for 32P and 125I
TranScreen LE images (compared to normal intensifying screens)

This is the first and only intensifying screen for 35S, 14C, TranScreen HE
33P, 45Ca, 59Fe and even 3H labelled samples. Improves the
resolution of 32P samples compared to traditional screens. q Increases sensitivity at least four-fold (compared
to normal intensifying screens)
TranScreen HE
q Improved handling features and image quality
Innovative intensifying screen technology for 32P, 125I and (compared to BioMax MS Screens)
high energy emitting radiolabels.
Low Energy TranScreen LE High Energy TranScreen HE

3H 14C 35S 33P 32P 32P & 125I Sample

Phosphor

Base

7 mil BioMax MS Film
Estar Base

Reflector

Hypercassette Active side FIG 16
of blot Principle of TranScreen Intensifying Screens. Note better
{Phoshor resolution of TranScreen LE
BioMax MS film
TranScreen Surface
Folder Applications
Reflector surface
q 3H and 14C thin layer chromatography
FIG 15 q Southern, Northern and Western blots
Configuration of film, screen and sample when in use. q Sequencing with 35S and 33P

Ordering information Size Quantity
8x10in 1 screen
Kodak BioMax TranScreen LE 35x43cm 1 screen
Code
V1622034 Quantity
V8660789 1 screen
1 screen
Kodak BioMax TranScreen HE Size
8x10in
Code 35x43cm
V8563959
V8811457

16 .

Hypercassette AUTORADIOGRAPHY
CASSETTES

Product description Features and benefits

Hypercassette™ Autoradiography Cassettes have been q Light tight exposures at temperatures as low as –70°C
designed for the toughest demands of life science
research. Deep cassettes are 1.5cm in depth allowing q Long life, chip proof surfaces
them to accommodate thicker samples.
q Optimum resolution from a design that ensures
maximum, even contact between film and sample

q Minimal contamination as the non-porous pad gives
a wipe clean interior

q Colour coding of work possible, choice of
Hypercassette colours from red, blue or neutral

q Record details on polycarbonate label and wipe clean
after use

FIG 17
Hypercassette Autoradiography Cassettes

Ordering information

Hypercassette, neutral (standard)

Code Size Quantity Hypercassette, blue (standard)
RPN11642 18x24cm 1 cassette
RPN11643 24x30cm 1 cassette Code Size Quantity
RPN11644 30x40cm 1 cassette RPN13642 18x24cm 1 cassette
RPN11645 35x43cm 1 cassette RPN13643 24x30cm 1 cassette
RPN11646 18x43cm 1 cassette RPN13645 35x43cm 1 cassette
RPN11647 20x40cm 1 cassette RPN13649 8x10in 1 cassette
RPN11648 5x7in 1 cassette
RPN11649 8x10in 1 cassette
RPN11650 10x12in 1 cassette

Hypercassette, red (standard)

Code Size Quantity Hypercassette, neutral (deep)
RPN12642 18x24cm 1 cassette
RPN12643 24x30cm 1 cassette Code Size Quantity
RPN12645 35x43cm 1 cassette RPN11628 18x24cm 1 cassette
RPN12649 8x10in 1 cassette 1 cassette
RPN11627 30x40cm 1 cassette

RPN11629 8x10in

17 .

Amplify FLUOROGRAPHIC REAGENT

Product description Features and benefits

Amplify™ is a fast, convenient fluorographic reagent q Increased detection efficiency
for 35S, 14C and 3H labelled samples in gels. Increased • 1000 fold for 3H • 15 fold for 35S and 14C
sensitivity can be achieved in addition to considerably
reducing exposure times of these weak beta emitters. q Less than 2 hours total preparation time

35S – 3H – q Odourless, does not contain DMSO or other
+ + organic solvents

q Versatile, can be used with acrylamide or agarose
gels with no change in protocol

q Fully compatible with Sensitize and Hyperfilm MP

FIG 18
Autoradiographs showing the detection of
35S- and 3H-labelled proteins in acrylamide
gels with (+) and without (–) Amplify. Samples
exposed to pre-flashed Hyperfilm MP

emulsion plastic base
emulsion
anti-scratch
layer

gel

3H/Auger 3H/Auger

AB

FIG 19
Internal absorption of weak β-emissions within a sample (A) compared to gel impregnated with Amplify (B). With Amplify β-emissions
are converted to light and efficiently recorded on film

Ordering information Size Quantity
1 litre 1 pack
Amplify Fluorographic Reagent
Code
NAMP100

18 .

lam pDARKROOM

Product description Features and benefits

The Darkroom Lamp is a socket type metal lamp that q Socket type metal lamp that provides direct illumination
provides direct illumination. It screws into either a wall
or drop-cord socket and holds a 5.5 inch diameter filter FIG 20
and a 7.5 watt standard incandescent bulb.The GBX-2 Darkroom Lamp
Safelight Filter is compatible with all autoradiography
films including the highly light sensitive BioMax MS film. Quantity
A 7.5 or 15 watt bulb is recommended. 1 lamp

Ordering information Quantity
1 filter
Darkroom Lamp

Code Size
V1521178 14cm (51/2 in) diameter filter

Note: Bulb and filter not included with Darkroom Lamp

Safelight Filter, GBX-2 Size
14cm (51/2 in) diameter
Code
V1416627

Hypertorch Features and benefits

Product description q Local illumination, minimizes film background
q Red LED, compatible with most autoradiography films
Hypertorch™ is a red light darkroom torch. A red q Portable, allows all areas of darkroom to be illuminated
LED produces a safe wavelength of light for short q Spring switch prevents accidental exposures
exposures to film. It allows visibility in areas of
the darkroom, where fixed safe lights cannot reach
e.g. cupboards. Ideal for use with Hyperfilm, Kodak
autoradiography films and emulsions.

Ordering information Size FIG 21
3 torches Hypertorch – battery-powered, darkroom pen torch
Hypertorch
Code Quantity
RPN1620 1 pack
(includes 2 AAA batteries)

19 .

ECL Mini-Camera

Product description Features and benefits

The ECL™ Mini- Camera is a camera luminometer q Instant results at the bench on Polaroid film
specifically designed for use with ECL Systems, in q Compact and portable
particular Western blots from mini gels. It allows the q Handles blots up to a size of 52x77mm
chemiluminescent signal to be captured on Polaroid™
film without the need of a darkroom.

Polaroid film 667 is recommended for routine work
while 612 can be used if greater sensitivity is needed.
For work that requires the highest sensitivity and resolution
use Hyperfilm ECL and conventional methodology.

Applications

q Chemiluminescent blots from mini gels

FIG 23
ECL Mini-Camera

FIG 22 Quantity
Doubling dilution (31, 15.5, 7.8, 3.9 ng) of mouse 1 camera
IgG detected with anti-mouse Ig HRP and ECL Plus.
Exposed for 1 minute Quantity
50 boats
Ordering information

ECL Mini-Camera
Code
RPN2069
(Supplied with 5 sample boats)
Polaroid film is not supplied with the ECL Mini-Camera

Replacement Sample Boats
Code
RPN2079

20.

Hypercoat NUCLEAR EMULSIONS

Product description Features and benefits

Hypercoat™ EM-1 and Hypercoat LM-1 are ready- q Ready-to-use, simply melt before dipping and coating
to-use nuclear emulsions that can be used in the
microautoradiography of radiolabels in tissues or cells. q High resolution, uniform crystal size and aggregate free
When using 3H the low energy of this isotope gives results
of the highest resolution best appreciated using Hypercoat q Maximum sensitivity, high silver content allows reduced
EM-1 which produces grains of a fine size. Isotopes exposure times (0.09g Ag/g emulsion)
such as 14C, 35S, 33P, 32P and 125I are best detected with
Hypercoat LM-1 which has a slightly larger grain size q Crystal sizes to suit all applications
optimized for use with higher energy emissions. Both – 0.2µm for general microautoradiography
emulsions come ready-to-use and do not require diluting, (Hypercoat LM-1)
a process that can increase backgrounds. Store in the dark – 0.13µm for high resolution light or electron
at 2-8°C. Compatible with Hypercoat dipping vessels, microscopy of 3H (Hypercoat EM-1)
specially designed glass vessels that facilitate maximum
slide coverage from small volumes of emulsions.

Applications

q Microautoradiography of tissues and cells

FIG 24 FIG 25
Binding of [125I]endothelin-1 Hypercoat Nuclear Emulsions and Hypercoat Dipping Vessel
to artery wall of patient
with ischaemic heart disease,
visualized with Hypercoat
LM-1

Photograph courtesy of Dr M
Dashwood, Department of Physiology,
Royal Free Hospital School of
Medicine, London

Ordering information Code Size Quantity
RPN40 2x20ml 1 pack
Product RPN41 2x20ml 1 pack
Hypercoat LM-1 RPN39 1 pack 3 vessels
Hypercoat EM-1
Hypercoat Dipping Vessels

21.

Hyperprocessor AUTOMATIC
FILM PROCESSOR

Product description Features and benefits

Hyperprocessor™ is an advanced automatic film q Simple to operate. Develops, fixes, washes and dries
processor of compact bench top design. It is designed automatically in 21/2 mins
to give consistent high quality autoradiographs simply
and quickly with Hyperfilm MP, Hyperfilm ECL, BioMax, q Reproducible results. Internal control systems ensure
XAR, XLS and most standard X-ray films. consistent high performance
Sensors measure the film throughput and replenish
chemicals as needed. Chemicals are maintained at q Compact design, requires less than 0.2m2 floor space
optimum temperatures for quality results.
q Versatile. Can handle film sizes from 10x10cm up to
Applications 35x43cm

q Automatic rapid processing of autoradiography films q High throughput. Can process 70 films, size 24x30cm,
per hour

FIG 26
Hyperprocessor

Ordering information Quantity
1
Contents 2
1
Component 1
Trolley 1
Reservoirs
Small spares kit Size
Full operator manual 220–240v
Full installation manual 110–120v

Product Code Quantity
Hyperprocessor RPN1700 1 processor
Hyperprocessor RPN1700A 1 processor

ADDITIONAL INFORMATION Note: Hyperprocessor is not available in 22.
certain countries. Please contact your local office for further details.

CocktailsLIQUID SCINTILLATION

Product description – PCS - Wide sample range phase-combining scintillant.
Efficiencies with 3H of 30-50%. Flashpoint 27.2°C.
A comprehensive range of liquid scintillation cocktails
compatible with most sample types. q For non-aqueous samples:
– BCS-NA - Biodegradable scintillant. Efficiencies with 3H
Features and benefits of up to 45%. Low odour, low toxicity and a high
flashpoint (>150°C) makes it less hazardous to the user
q For aqueous samples: and the environment.
– ACS - Organic scintillant. Efficiencies with 3H of up to 50%, – OCS – High efficiency xylene-based scintillant. Efficiency
provided sample added is less than 6% (v/v). Flashpoint 27.2°C. with 3H of >50%. Flashpoint 26.7°C.
– ACSII - Organic scintillant. Efficiencies with 3H of up to 50%,
provided sample added is less than 4.5% (v/v). q Tissue solubilizer:
A flashpoint of 48.9°C reduces handling hazards. – NCS – Rapid and efficient solubilizer for biological materials
– BCS - Biodegradable scintillant. Efficiencies with 3H of and polyacrylamide gels prior to liquid scintillation counting.
30-40%. Low odour, low toxicity and a high flashpoint (>150°C) Compatible with BCS-NA and OCS scintillation cocktails
makes it less hazardous to the user and the environment.

Ordering information Code FIG 27
NACS104 Liquid Scintillation
Product* NACS204 Cocktail specifications
ACS Scintillation Cocktail NBCS104
ACS-II Scintillation Cocktail NPCS104 Pack Size
BCS Scintillation Cocktail NBCS204 4x4litres
PCS Scintillation Cocktail NOCS104 4x4litres
BCS-NA Scintillation Cocktail NNCS502 4x4litres
OCS Scintillation Cocktail 4x4litres
NCS Tissue Solubilizer Code 4x4litres
CFR6 4x4litres
Product CFR8 500ml
[14C] Scintillation Standard, N-hexadecane TRR6
[14C] Scintillation Standard,Toluene, TRR8 Pack Size
[3H] Scintillation Standard, N-hexadecane, TRR9 75kBq, 2.0mCi, 1x106dpm/g
[3H] Scintillation Standard,Toluene CFR101 75kBq, 2.0mCi, 1x106dpm/g
[3H] Scintillation Standard,Water TRR101 260kBq, 7.0mCi, 4x106dpm/g
[14C] Sample Oxidiser Standard 260kBq, 7.0mCi, 4x106dpm/g
[3H] Sample Oxidiser Standard 333kBq, 9.0mCi, 4x106dpm/g
5000 dpm
10000 dpm

23. *ADDITIONAL INFORMATION Scintillation cocktails are not available in
certain countries, please contact your local office for availability.

Selection guide

24.

Microscale AUTORADIOGRAPHY
S TA N DA R D S

Product description Features and benefits

Microscale™ Standards are reference sources for q Safe and easy to use as the radioisotope exists as
autoradiography.The 3H, 14C or 125I activity has been a stable polymer
arranged in eight or ten layers of polymer, to give
a good working range. q Activity is uniformly incorporated into the polymer

Applications q Arranged in 8 to 10 layers of increasing specific activity,
to provide a good working range
q Whole body autoradiography
q Receptor localization and receptor binding

[3H]Microscales RPA507 [14C]Microscales RPA511 [125I]Microscales
RPA506 Bq/mg nCi/mg RPA504 Bq/g nCi/g RPA523
Bq/mg nCi/mg kBq/g nCi/g 3635.0 98.26 kBq/mg nCi/mg
1793.0 48.46 23.9 646
4048.0 109.4 588.0 15.9 31.89 862 11.9 321
25.90 700 888.7 24.02
2468.0 66.7 290.0 7.84 352.6 9.53 5.85 158
177.2 4.790 3.0 81
1487.0 40.2 180.0 4.87 19.39 524 87.21 2.357 1.33 36
33.63 0.909 0.74 20
872.8 23.6 74.4 2.01 12.91 349 16.02 0.433 0.37 10
0.21 5.6
525.4 33.5 37.0 1.00 8.55 231 9.29 0.251 0.095 2.56
3.70 0.100 0.044 1.20
320.4 8.7 17.8 0.48 4.44 120

175.0 4.7 9.62 0.26 2.18 59

110.6 3.0 3.74 0.10 1.11 30

FIG 28
Typical polymer activity

Ordering information

Product Code Size Quantity
Autoradiographic RPA506 0.111–4.07kBq/mg, 3–110nCi/mg 20 strips (19mmx3.5mmx50µm)
[3H] Microscale RPA507 3.7–592Bq/mg, 0.1–16nCi/mg 20 strips (19mmx3.5mmx50µm)
RPA510 10 strips RPA506 + 10 strips RPA 507 10 + 10 strips
Autoradiographic RPA504 1.15–32.7kBq/g, 31–883nCi/g 3 strips (19mmx3.5mmx120µm)
[14C] Microscale RPA504L 1.15–32.7kBq/g, 31–883nCi/g 20 strips (19mmx3.5mmx120µm)
RPA511 3.7–3700Bq/g, 0.1–100nCi/g 3 strips (21mmx3.5mmx120µm)
Autoradiographic RPA511L 3.7–3700Bq/g, 0.1–100nCi/g 20 strips (21mmx3.5mmx120µm)
[125I] Microscale RPA522 0.046-23.7kBq/mg, 1.25-640nCi/mg 1 block (25mmx4.5mmx25µm)
RPA523 0.046-23.7kBq/mg, 1.25-640nCi/mg 3 strips (25mmx4.5mmx20µm)
RPA523L 0.046-23.7kBq/mg, 1.25-640nCi/mg 20 strips (25mmx4.5mmx20µm)

25.

Sensitize PREFLASH UNIT

Product description Features and benefits

Sensitize™ is a specially designed flashgun for use with q Most effective when detecting small amounts
Hyperfilm ECL or Hyperfilm MP in conjunction with of radioactivity and chemiluminescent signal
Hyperscreen Intensifying Screens to improve sensitivity
and quantitation. It improves the linear range and q Calibration protocol ensures reproducibility
increases film sensitivity.
q High quality orange filter for optimum wavelength

q Sealed unit guards against white light leakage,
often a problem with a conventional flashgun

+–

0.56kb FIG 30
Sensitize Pre-f lash Unit
FIG 29
λ Hind III digests (10-fold dilutions) ECL detected and exposed to
Hyperfilm ECL. Note the presence of 0.56kb fragment after pre-
flashing

Ordering information Quantity
1 unit
Sensitize Preflash Unit
Code
RPN2051

26.

PROCESSING Hanger

Product description Features and benefits

Clip-type metal film hanger for manual deep tank q Design ensures optimum film tension
development of sheet films.
q Accurate alignment of pin hole clamps permits
easier washing near clips

Ordering information FIG 31 Quantity
Processing 1 hanger
Processing Hangers Hanger 1 hanger
Code 1 hanger
V1502723 Size
V1502764 5x7in
V1502822 8x10in
35x43cm

TrackerTape

Product description Features and benefits

TrackerTape™ is a phosphorescent tape which, when q Produces a permanent, negative image on
written on, can be used to label samples and corresponding autoradiographs
autoradiographs.
q Avoids confusion by allowing samples to be
Applications matched to results

q Southern, Western and Northern blots q Can be used with ordinary marker pen
q Quick to use, image is produced in minutes

Ordering information Size FIG 32
10x15cm Western blot identified
TrackerTape
Code by TrackerTape
VRPN2050
Quantity
10 sheets

2 7. ADDITIONAL INFORMATION Note: Not available in the USA.

KODAK Duplicating FILM

Product description Features and benefits

A range of duplicating products which facilitate the RA Duplicating Film
generation of direct positive film transparencies q Slower film for enhanced image quality and film stability
of electrophoresis gels.

Applications

q RA Duplicating Film – single-emulsion film on a
clear base that enables same-size reproductions of
autoradiographs

FIG 33
32P labelled blot
reproduced on
RA Duplicating Film

Ordering information

Product Code Size Quantity
RA Duplicating Film V1429760 8x10in 100 sheets
V8165938 28x35cm 100 sheets
V1389923 35x43cm 100 sheets

28.

KODAK micro-autoradiography

& TEM PRODUCTS

Product description Features and benefits

A specially engineered range of micro-autoradiography q Suitable for medium energy electrons (40-100kV)
products that facilitate high-quality images in Transmission q Range offers flexibility to meet most user needs
Electron Micrography (TEM) and electron diffraction q Films have gelatin overcoat to minimise abrasion
studies. Each of these products offers considerable
latitude in performance, but they are best suited for and a clean gelatin backing to reduce curl
medium-energy electrons (40 to 100kV), although
acceptable results can also be obtained at higher FIG 34
electron-energy levels. The Kodak TEM range

Kodak Electron Microscope Film 4489
This film has approximately half the speed of SO-163 film but
exhibits less curl and shorter pump-down times.

Kodak Electron Image Film SO-163
Approximately two times faster than 4489 film, however
it requires longer pump-down times and shows greater
film curl. When using SO-163 film for the first time,
recalibration of the microscope exposure device is
recommended.

Ordering information Size Quantity
3.25 x 4in 100 sheets
Electron Microscope Film 4489 3.25 x 4in 250 sheets
6.5 x 9cm 100 sheets
Code 6.5 x 9cm 250 sheets
V1613108 8.3 x 11.9cm 100 sheets
V1662238 7.0 x 7.0cm 100 sheets
V1615228
V8971160 Size Quantity
V1725852 3.25 x 4in 100 sheets
V1608124 3.25 x 4in 250 sheets
6.5 x 9.0cm 100 sheets
Electron Image Film SO-163
Code
V1679257
V8010100
V1679273

29.

Amplify, ECL, ECL Plus, GeneImages, Hypercassette,
Hypercoat, Hyperfilm, Hyperfilm device, Hyperprocessor,
Hyperscreen, Hypertorch, ImageQuant, Megaprime,
Microscale, Personal Densitometer, Redivue, Sensitize,
Thermo Sequenase and TrackerTape are trademarks of
Amersham Biosciences Limited or its subsidiaries.

Kodak, BioMax, TranScreen and X-Omat
are trademarks of Eastman Kodak Company. Polaroid is
a trademark of Polaroid (UK) Limited. CDP-Star is a
trademark of Tropix Inc and protected under one or more
US patents numbers 5,851,771; 5,538,847; 5,326,882;
5,145,772; 4,978,614; and 4,931,569. All goods and services
are sold subject to the terms and conditions of sale of the
company within the Amersham Biosciences group
which supplies them. A copy of these terms and conditions
is available on request.

Amersham Biosciences UK Limited
Amersham Place Little Chalfont Buckinghamshire England
HP7 9NA

Amersham Biosciences AB
SE-75184 Uppsala Sweden

Amersham Biosciences Inc
800 Centennial Avenue PO Box 1327 Piscataway NJ
08855 USA

Amersham Biosciences Europe GmbH
Munzinger Strasse 9 D-79111 Freiburg Germany

© Amersham Biosciences UK Limited. 2001
– All rights reserved.

For more information visit our website:

www.amershambiosciences.com

Asia Pacific Denmark Latin America South East Asia
Tel: +852 2811 8693 Tel: 45 16 24 00 Tel: +55 11 3667 5700 Tel: 60 3 8024 2080
Fax: +852 2811 5251 Fax: 45 16 24 24 Fax: +55 11 3667 8799 Fax: 60 3 8024 2090

Australasia Finland & Baltics Middle East and Africa Spain
Tel: +61 2 9894 5152 Tel: +358-(0)9-512 39 40 Tel: +30 (1) 96 00 687 Tel: 935 944 950
Fax: +61 2 9899 7511 Fax: +358-(0)9-512 17 10 Fax: +30 (1) 96 00 693 Fax: 935 944 955

Austria France Netherlands Sweden
Tel: 01 57 606 1623 Tel: 01.69.35.67.00 Tel: 0165 580 410 Tel: 018 612 1900
Fax: 01 57 606 1627 Fax: 01.69.41.96.77 Fax: 0165 580 401 Fax: 018 612 1910

Belgium Germany Norway Switzerland
Tel: 0800 73888 Tel: 0761 49 03 403 Tel: 23 18 58 00 Tel: 01 802 81 50
Fax: 03 272 16 37 Fax: 0761 49 03 405 Fax: 23 18 68 00 Fax: 01 802 81 51

Canada Italy Portugal UK
Tel: 1 800 463 5800 Tel: 02 27322 1 Tel: 21 417 70 35 Tel: 0800 616 928
Fax: 1800 567 1008 Fax: 02 27302 212 Fax: 21 417 31 84 Fax: 0800 616 927

Central East & S.E. Europe Japan Russia & other C.I.S./N.I.S. USA
Tel: +43 1 982 3826 Tel: 81 3 5331 9336 Tel: +7 (095) 232 0250 Tel: +1 800 526 3593
Fax: +43 1 985 8327 Fax: 81 3 5331 9370 Fax: +7 (095) 230 6377 Fax: +1 877 295 8102