Implementation_Manaul_XS_Series_MKT-30-1009

EXPLORER (Stored Data)

I. Finding a Sample

1. Click [EXPLORER] icon or press [F7]. Make sure you are in the 10,000
Stored Data File, and not Last 20.

2. Click [Record] from the Tool Bar and select [First] to move to the top of the
stored data.

3. Click [Edit] from Tool Bar and select [Find] or press and hold [Ctrl+F] on the
keyboard. The Find Input dialog box is displayed.

4. Enter the search criteria. (If you are using barcodes with leading zeros before
the numbers, these zeros must be entered also.)

5. “*” or “?” can be used as a wildcard for the following situation:
? – In place of a single digit for an unknown number or letter.
* – Use for multiple unknown letters or numbers and placed at the end of a string.

6. Press [Enter] on the keyboard or click [Next] to search below that sample
number in the list. The data will appear in the list with cursor on it. Click [Close].

II. Edit Sample ID# or Patient ID

1. Click [EXPLORER] icon or press [F7]. Make sure you are in the 10,000
Stored Data File, and not Last 20.

2. Click on the Sample number to be edited.
3. Click [Validate] or press [F11] to unvalidate the sample. (‘V’ disappears in

the far left column of the Sample Information List for this sample).
4. Click [Edit], then [Modify] from Tool Bar or press [F10] on the keyboard. The

Modify dialog box will appear with that specific Sample ID.
5. Overwrite the current information with the edited information.
6. Click [OK] and the new sample information will be stored. Screen displays

Stored Data List with cursor on that edited sample.
7. Click [Validate] or press [F11] to re-validate the sample and the corrected

results are printed and retransmitted to the LIS.

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XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 11 of 34

III. Reprint to Graphic printer or Retransmit data to Host

1. Click [EXPLORER] icon or press [F7]. Make sure you are in the 10,000
Stored Data file, and not Last 20.

2. Click on the Sample number to reprint or retransmit.

¾ To print multiple samples in a row, click on the 1st sample to highlight it
in blue, hold down the “Shift” key, and then click on the last sample to be
printed/transmitted.

¾ To print multiple samples that are not in a row, hold down the “Ctrl” key
and click on all that you want to reprint/retransmit.

3. Click [Report] from Tool Bar and select “Report (GP)(R)” to reprint or choose
“Host (HC)(H)” to retransmit to a host computer or press [F12] Output and
press #3 Report GP for a graphic printout or press #1 to retransmit this data
to a host computer.

Note: Cannot reprint nor retransmit with Last 20 selected or if data is unvalidated.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 12 of 34

XS-1000i without Sampler
Competency Assessment

Identify the parts indicated below:

1. The sample size for aspiration on the XS is ______________.

2. Reagents are stable for _________ days after opening.

3. When performing instrument start up, turn on the _____________ first
and then the ______________.

4. Processing samples in the capillary mode requires a ________ dilution.

5. You want to retransmit or reprint a result, but under “Report” it does not give you the
option – why?
a. Last 20 is selected
b. The GP printer is out of paper
c. The sample is not validated
d. Both a and c are correct

6. QC material should be brought to __________________ and hand mixed prior to
processing. Vials are good for _______________ days after opening.

7. CELLCLEAN™ is _________________________ and is used for the Monthly Rinse
Procedure and Rinse Flow Cell Procedure.

8. The Air Pump is changed every _______ cycles and is accessed from the ________
of the Main Unit.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 13 of 34

XS-1000i without Sampler Competency Assessment

Match the reagent with its function:

_____ EPK 1. Lysing reagent used for differential and optical WBC

_____ FFD 2. Diluent

_____ FFS 3. Cyanide free hemoglobin reagent

_____ SLS 4. Fluorescent dye used in differential reaction

Match the Menu Option with its function:

_____ Sample Explorer 1. Displays scattergrams & histograms with patient results

_____ Controller 2. Displays patient results in line format

_____ Data Browser 3. Contains such options as Maintenance, Reagent Log, Settings

_____ Auto Rinse 4. Cycles all reagents 3-5 times to obtain a background

Using the terms listed, label the X and Y-axis and cell populations
on the Diff scattergram below:

1. Basophils
2. Eosinophils
3. Fluorescence
4. Ghost Cells
5. Lymphocytes
6. Monocytes
7. Neutrophils
8. Side Scatter

Using the terms listed, label the flagging areas on the Diff scattergram below:

1. IG?
2. NRBC?
3. PC?
4. Blast/Atypical Lympho?
5. LS?
6. Abn/Lympho?

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 14 of 34

XS-1000i without Sampler
Competency Assessment Key

Identify the parts indicated below:

Ready LED

IPU Open/Close
Button
Start
Button Sample
Position Cover

1. The sample size for aspiration on the XS is 20 µl or 67 µl in capillary mode.

2. Reagents are stable for 60 days after opening.

3. When performing instrument start up, turn on the IPU first and then the Main Unit.

4. Processing samples in the capillary mode requires a 1:7 dilution.

5. You want to retransmit or reprint a result, but under “Report” it does not give you the
option – why?
a. Last 20 is selected
b. The GP printer is out of paper
c. The sample is not validated
d. Both a and c are correct

6. QC material should be brought to room temperature and hand mixed prior to
processing. Vials are good for 14 [(e-CHECK(XS)] or 7 (e-CHECK) days after
opening.

7. CELLCLEAN™ is 5% Sodium Hypochlorite solution and is used for the Monthly
Rinse Procedure and Rinse Flow Cell Procedure.

8. The Air Pump is changed every 30,000 cycles and is accessed from the top of the
Main Unit.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 15 of 34

XS-1000i without Sampler Competency Assessment Key

Match the reagent with its function:

2 EPK 1. Lysing reagent used for differential and optical WBC

1 FFD 2. Diluent

4 FFS 3. Cyanide free hemoglobin reagent

3 SLS 4. Fluorescent dye used in differential reaction

Match the Menu Option with its function:

2 Sample Explorer 1. Displays scattergrams & histograms with patient results

3 Controller 2. Displays patient results in line format

1 Data Browser 3. Contains such options as Maintenance, Reagent Log, Settings

4 Auto Rinse 4. Cycles all reagents 3-5 times to obtain a background

Using the terms listed, label the X and Y-axis and cell populations
on the Diff scattergram below

3 1. Basophils
6 2. Eosinophils
3. Fluorescence
5 7 2 4. Ghost Cells
8 5. Lymphocytes
1 6. Monocytes
4 7. Neutrophils
8. Side Scatter

Using the terms listed, label the flagging areas on the Diff scattergram below

6 1. IG?
2. NRBC?
1 3. PC?
4 4. Blast/Atypical Lympho?
5. LS?
5 6. Abn/Lympho?

23

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 16 of 34

XS-1000i with Sampler Training Outline

I. XS-1000i with Sampler System Configuration

• Information Processing Unit (IPU)

• Main Unit (MU)

• Laser Printer

II. XS-1000i with Sampler Basic Parts

A. Main Unit External Parts
1. Power Switch
2. Start/Stop Switch
3. Ready LED
4. Sample Tube Adaptors
5. Hand-held Barcode Reader
6. ID Barcode Reader
7. Locking Screw
8. Sampler

B. Main Unit Internal Parts Aspirates sample for testing

1 Piercer Needle Rinses manual aspiration tip (inside and out)
2 Rinse Cup
3 WBC/DIFF Reaction Prepares diluted samples for analyzing WBC
and DIFF. Samples are then sent to optical
Chamber detector block.

4 RBC/PLT Detector Impedance counting enhanced with
hydrodynamic focusing
5 Trap Chamber
6 Air Pump Check daily for fluid accumulation
7 Manual Start Switch
8 Mixing Arm and Replaced every 30000 cycles

Tube Sensor
9 Rack and Lid Close

Sensor

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 17 of 34

XS-1000i with Sampler Training Outline

II. XS-1000i Basic Parts (continued)

C. Graphic Display
1. Review Information

• POSITIVE/NEGATIVE
¾ Positive (backlit red) requires review
Diff – WBC differential count abnormality
Morp – Abnormal Cell Morphology (WBC or RBC)
Count – Abnormal Blood Cell Count
¾ Negative (backlit green) no abnormalities

• ERROR (backlit red) E
¾ Func. : Indicates analysis error not caused by sample.
¾ Result : Indicates error has been caused by the sample, such as
“Low Blood Volume”.

2. Flags

@ Result falls outside of instrument linearity limits

* Data may be unreliable due to abnormality in sample

+ /- Data outside reference interval

Note: Above indicators are in order of priority.

3. Action Display

• Double-click the “Action” button (in red) on Browser screen to display
the details of the action to be taken.

¾ Count DIFF-CH = to improve the accuracy of the white count,
add DIFF to the discrete testing, and perform the analysis again.

D. Linearity vs. Display Range

Parameter Linearity Display
WBC 0 - 400 x 103/µL 0.00-999.99 x 103/µL
RBC 0 - 8.0 x 106/µL 0.00-99.99 x 106/µL
HCT
HGB 0 - 60 % 0.0-100.0 %
PLT
0 - 25 g/dL 0-30.0 g/dL
0 - 5000 x 103/µL 0-9999 x 103/µL

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 18 of 34

XS-1000i with Sampler Training Outline

E. Function Keys
• F1 – Help (also used to silence alarm)
• F2 – Manual
• F4 – Main Menu
• F5 – QC Files
• F6 – Worklist
• F7 – Explorer
• F8 – Browser

III. Reagents

A. Message Displayed “Replace Container -------“
EPK CELLPACK™
FFD STROMATOLYSER-4DL™
FFS STROMATOLYSER-4DS™
SLS SULFOLYSER™

Note: Replace reagents only when Message is displayed. Open dating for all
reagents is 60 days.

B. Replacing a Reagent
1. When XS alarms, press [F1] to silence the alarm. The Help dialog box will
display the reagent that needs to be replaced.
2. Click [Execute] to open Reagent Replacement screen.
3. Verify which reagent to change.
4. Scan the barcode (EAN-128) on the reagent box with the hand-held
barcode reader. The updated information is displayed. If the barcode
cannot be read, the information can be entered manually.
5. Replace the reagent container using clean technique to avoid
contamination.
6. Click [Execute] on the reagent replacement screen to register the
reagent replacement onto the reagent log and start aspiration of the
reagent.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 19 of 34

XS-1000i with Sampler Training Outline

IV.Start Up XS-Series

Power Up Sequence:
1 – IPU, 2 – Main Unit, 3 – Printer (if turned off)

IPU: Power on IPU, when the log-on box for the XS-Series program
appears, type “XS” for the User Name and then press [Enter].
There is no password

Main Unit: 1. Do not turn on the Main unit until after the log-on screen on the
IPU displays. Switch is located on the upper right hand side of
the Main Unit.

2. Once the instrument is turned on it will perform several self
checks (microprocessor, mechanical, temperature and
background).

3. If the background check is successful, the “Analyzer Icon” at the
bottom of the IPU screen and the “Ready LED” on the analyzer
will turn green and the system is ready for analysis.

If the background exceeds limits, a Background Error message and the Blank
Check dialog box appear. Click [Accept] on the error dialog box to initiate
another Autorinse. Error disappears when background is lower than limits shown
in Acceptable Background Limits table.

Acceptable Background Limits

RBC <0.02 x 106/µL
Hgb
PLT <0.1 x g/dL
WBC-C <10 x 103/µL
WBC-D <0.10 x 103/µL
<0.10 x 103/µL

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 20 of 34

XS-1000i with Sampler Training Outline

V. Quality Control

• QC material should be warmed to room temperature for 15-20 minutes.

• Mix controls by gentle end to end inversion until re-suspended.

• Do Not place control material on mechanical mixer or rocker.

• Commercial control files must be established prior to analysis.

Note: Open vial expiration is 14 days for e-CHECK(XS) (1.5 ml vial) and 7 days
for e-CHECK (4.5 ml vial).

Processing QC

A. Sampler Mode (barcoded e-CHECK 4.5 ml vials only)

1. With Ready LED green, click the [Sampler] icon or press [F3].

2. Open Sampler Cover.

3. Place QC vials into a sample rack in positions 8, 9, and 10 and place the
rack into sampler position 1 or 2. Rack notch must be facing right side of
instrument.

4. Verify appropriate sample tube adaptor is in place.

5. Click on the rack and tube position number in the Sampler Sample No. dialog
box to initiate where the instrument is to begin processing. Click [OK].

6. Close Sampler Cover and press the [START/STOP] button on the Main Unit.

7. A dialog box will display when sample analysis has completed. Click [OK].

B. Manual Mode (4.5 ml or 1.5 ml vials)

1. From the Main Menu, with Ready LED green, click the [Manual] icon or
press [F2].

2. Enter the QC lot number into the “Sample No.” field by one of the
following methods:

¾ using the handheld barcode reader or

¾ manually type in the number using the keyboard or

¾ click [QC] button in the Manual Sample No. dialog box and highlight
the control level and lot number to be analyzed.

Note: If manually typing the lot number, “QC” must always be upper
case and followed with a dash.

3. Click [OK] or press [Enter] on the keyboard. The QC dialog box opens.

4. Verify appropriate lot and level is selected.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 21 of 34

XS-1000i with Sampler Training Outline

V. Quality Control (continued)

5. Select the appropriate sample tube adaptor and place it in the sample
position area of the XS-1000i with the red mark facing up. Turn the
adaptor clockwise until there is a click (about 45 degrees) to secure it.

6. Place the QC sample into the sample adaptor and press the [START]
button on the right above the sample position cover.

7. The QC results will display in the dialog box when sample measurement
has completed. Out of range results will display with a red background.
Press [Accept] for results to plot, or [Cancel] to abort.

8. Repeat steps 1-7 for the remaining levels of QC to be analyzed.

Reviewing QC

A. Viewing Radar Charts

1. On IPU, click [QC Files] icon or press [F5].

2. Click the appropriate file number (1-20 or X-barM).

3. Once the file is selected, the Radar Chart will display on the right side of
the screen.

4. The control data is displayed in blue.

5. Date and time of analysis are displayed to the left in the “Analysis Date”
list.

6. Parameters that exceed acceptable limits will have a red “X” displayed on
the Radar chart. The parameter name will be backlit in red and an error
message will be displayed to the left of the file number.

B. Viewing L-J Charts

1. On IPU, click on [QC Files] icon or press [F5].

2. Double-click on the appropriate file (1-20 or X-barM). L-J chart will open
when selected.

3. Parameters that exceed acceptable limits will have a red “X” displayed as
the plotted point. The parameter name as well as the result value will be
backlit in red.

4. Scroll down to view remaining parameters using the scroll bar on the right
side of the screen or the down arrow on the keyboard.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 22 of 34

XS-1000i with Sampler Training Outline

VI.Sample Processing

Sample Aspiration Volumes –

♦ Manual Mode = 20 µL minimum volume = 500 µL or 90 µL
for micro collection
♦ Sampler Mode = 20 µL
♦ Capillary Mode = 67 µL minimum volume = 1 ml

minimum volume = 140 µL

A. Manual Mode

1. With “Ready LED” green click [Manual] icon or press [F2].

2. Enter sample ID # using the handheld barcode reader or manually type in
the information using the keyboard.

3. Select Discrete Testing Mode ONLY if Host is not providing that
information.

4. Click [OK] or press [Enter] on the keyboard.

5. Open the Sampler Cover.

6. Select the appropriate sample tube adaptor and place it in the sample
position area of the XS-1000i with the red mark facing up. Turn the
adaptor clockwise until there is a click (about 45 degrees) to secure it.

7. Place well-mixed sample into the appropriate size adaptor.

Note: If using non-pierceable microtainers, the cap must be removed
prior to processing.

8. Press the white [START] button on the right near the sample position
inside the Sample Cover.

B. Sampler Mode

♦ Label placement – Ensure labels are smooth with no loose edges.
1. When “Ready LED” is green, click [Sampler] icon or press [F3].
2. Open Sampler Cover.
3. Place patient sample(s) into rack(s) and position rack(s) in sampler. Rack

notch must be facing right side of instrument.
4. Verify appropriate sample tube adaptor is in place.
5. Click on the rack and tube position number in the Sampler No. dialog box

to initiate where the instrument is to begin processing. Click [OK].
6. Close Sampler Cover and press [START/STOP] button on the Main Unit.
7. A dialog box will display when sample analysis has completed. Click [OK].

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 23 of 34

XS-1000i with Sampler Training Outline

C. STAT Analysis in Sampler Mode with Sampler Interruption:

1. To interrupt Sampler operation to process a STAT in the Manual Mode,
press the [START/STOP] switch on the Main Unit. “Ready LED” will flash
orange. Processing will stop once the aspirated sample has been
completed.

Note: If interrupted before the sample is moved to the aspiration
position, the tube is placed back in the rack and STAT analysis
becomes “Ready”.

2. With “Ready LED” green, click the [Manual] icon or press [F2] on the
keyboard.

3. Enter the sample ID number in the “Sample Number” field using the
keyboard or handheld barcode reader.

4. Click [OK] or press [Enter] on the keyboard.

5. Open the Sampler Cover.

6. Select the appropriate sample tube adaptor. Place the adaptor into the
sample position area of the XS-1000i with the red mark facing up. Turn
the adaptor clockwise until there is a click (about 45 degrees) to secure it.

7. Place well-mixed sample into the appropriate size adaptor.

Note: If using non-pierceable microtainers, the cap must be removed
prior to processing.

8. Press the white [START] button on the right rear the sample position
inside the Sampler Cover.

9. Once completed, remove the sample from the sample position and verify
that the appropriate sample tube adaptor is in place.

10. Close the Sampler Cover.

11. When the “Ready LED” and the “Analyzer Icon” on the IPU turn green,
press the [START/STOP] button on the Main Unit to resume operation in
the Sampler mode.

12. A dialog box will display when sample analysis has completed. Click [OK].

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 24 of 34

XS-1000i with Sampler Training Outline

D. Capillary Mode
1. Prepare 1:7 dilution (20µL whole blood + 120 µL CELLPACK™).
2. With “Ready LED” green, click the [Manual] icon or press [F2] on the
keyboard.
3. Enter the sample ID number in the “Sample Number” field using the
keyboard or handheld barcode reader.
4. Select [YES] at the Capillary prompt for the mode of analysis.
5. Select discrete test order if this information is not being provided by the host.
6. Click [OK] or press [Enter] on the keyboard.
7. Open the Sampler Cover.
8. Select the appropriate sample tube adaptor and place it in the sample
position area of the XS-1000i with the red mark facing up. Turn the
adaptor clockwise until there is a click (about 45 degrees) to secure it.
9. Place the well mixed 1:7 dilution into the sample adaptor and press the
white [START] button on the right near the sample position inside the
Sampler Cover.
10. The XS will automatically multiply the results by 7.
11. Once testing is completed, reset the capillary mode radio button back to [NO].

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 25 of 34

XS-1000i with Sampler Training Outline

VII. Maintenance

A. Daily Shutdown – Performed Daily to clean detector and dilution lines

1. Click [Shutdown] icon on the Menu screen.

2. Click [Execute] to begin the process. The procedure will take
approximately 2 minutes to complete.

3. Once completed, the Shutdown dialog box will be replaced by the
Power off dialog box.

4. Click [Restart] to initiate an Autorinse and continue sample analysis
or perform a complete power off, if further testing is not going to be
done. (This is recommended at least once a week.)

5. If performing a complete “Power Off”, power off the MU, and then on
the IPU, click [File], [Exit].

6. Click [YES] to exit the XS program.

7. Click [Start] at the bottom of the Windows desktop.

8. Click [Shutdown] in the [Start] Menu.

9. A window appears with the option to choose shutdown. Press [Enter].
This allows the system to write unsaved data to the hard drive and
power itself off.

B. Monthly Rinse – Every 1200 Cycles or Monthly

1. Click [Controller] icon on the Main Menu.

2. Click [Maintenance] icon.

3. Click [Monthly Rinse] icon a dialog box will appear.

4. Open the Sampler Cover.

5. Place a tube containing a minimum of 3 mls of CELLCLEAN™
(5% Sodium Hypochlorite Solution) into the sample position with the
appropriate sample tube adaptor.

6. Press the white [START] button on the right near the sample position
inside the Sampler Cover to initiate cleaning.

7. Close the Sampler Cover.

8. Once completed the Monthly Rinse dialog box is replaced by the
Power off dialog box.

9. Click [Restart] to initiate an Autorinse and continue sample analysis
or perform a complete power off if further testing is not going to be
done.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 26 of 34

XS-1000i with Sampler Training Outline

VII. Maintenance (continued)

C. Every 30,000 Cycles
1. Change Piercer
2. Replace Air Pump

D. Setting Cycle Counters
1. Click [Controller] icon on the Main Menu.
2. Click [Maintenance] icon on the Controller Menu.
3. Click [Counter] icon on the Maintenance Menu.
4. Click [Reset] on the Piercer or Air Pump box, then click [OK] to reset
the counters to zero.
5. Click [Menu] on the Toolbar or [F4] to return to the Main Menu Screen.

E. As Needed Maintenance
1. Clog Removal
2. Clean RBC Aperture
3. Remove Air Bubbles
4. Rinse Flow Cell

VIII. Principles and Technology

RBC Hydrodynamic focusing + impedance
Hgb Sodium Lauryl Sulfate
Hct Cumulative Pulse Height measurement
MCV Calculated from Hct and RBC
PLT-I Hydrodynamic focusing + impedance
Side Scatter (internal complexity) &
Diff Channel Fluorescence (RNA/DNA)
Forward Scatter (cell size)
WBC-C Sideways fluorescent light
WBC-D

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

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This Page Left Blank Intentionally.

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XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 28 of 34

EXPLORER (Stored Data)

I. Finding a Sample

1. Click [EXPLORER] icon or press [F7]. Make sure you are in the 10,000
Stored Data File, and not Last 20.

2. Click [Record] from the Tool Bar and select [First] to move to the top of the
stored data.

3. Click [Edit] from Tool Bar and select [Find] or press and hold [Ctrl+F] on the
keyboard. The Find Input dialog box is displayed.

4. Enter the search criteria. (If you are using barcodes with leading zeros before
the numbers, these zeros must be entered also.)

5. “*” or “?” can be used as a wildcard for the following situation:
? – In place of a single digit for an unknown number or letter.
* – Use for multiple unknown letters or numbers and placed at the end of a string.

6. Press [Enter] on the keyboard or click [Next] to search below that sample number
in the list. The data will appear in the list with cursor on it. Click [Close].

II. Edit Sample ID# or Patient ID

1. Click [EXPLORER] icon or press [F7]. Make sure you are in the 10,000
Stored Data File, and not Last 20.

2. Click on the Sample number to be edited.
3. Click [Validate] or press [F11] to unvalidate the sample. (‘V’ disappears in the

far left column of the Sample Information List for this sample).
4. Click [Edit], then [Modify] from Tool Bar or press [F10] on the keyboard. The

Modify dialog box will appear with that specific Sample ID.
5. Overwrite the current information with the edited information.
6. Click [OK] and the new sample information will be stored. Screen displays

Stored Data List with cursor on that edited sample.
7. Click [Validate] or press [F11] to re-validate the sample and the corrected

results are printed and retransmitted to the LIS.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 29 of 34

III. Reprint to Graphic printer or Retransmit data to Host

1. Click [EXPLORER] icon or press [F7]. Make sure you are in the 10,000
Stored Data file, and not Last 20.

2. Click on the Sample number to reprint or retransmit.

¾ To print multiple samples in a row, click on the 1st sample to highlight it
in blue, hold down the “Shift” key, and then click on the last sample to be
printed/transmitted.

¾ To print multiple samples that are not in a row, hold down the “Ctrl” key
and click on all that you want to reprint/retransmit.

3. Click [Report] from Tool Bar and select “Report (GP)(R)” to reprint or choose
“Host (HC)(H)” to retransmit to a host computer or press [F12] Output and
press #3 Report GP for a graphic printout or press #1 to retransmit this data to
a host computer.

Note: Cannot reprint nor retransmit with Last 20 selected or if data is unvalidated.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 30 of 34

XS-1000i with Sampler Competency Assessment

Identify the parts selected below:

1. The sample size for aspiration on the XS is ______________.

2. Reagents are stable for _________ days after opening.

3. When performing instrument start up, turn on the _______________ first
and then the _____________.

4. Processing samples in the capillary mode requires a ________ dilution.

5. You want to retransmit or reprint a result, but under “Report” it does not give you the
option – why?
e. Last 20 is selected
f. The GP printer is out of paper
g. The sample is not validated
h. Both a and c are correct

6. QC material should be brought to _____________ and hand mixed prior to
processing. Vials are good for __________ days after opening.

7. CELLCLEAN™ is _____________ and is used for the Monthly Rinse Procedure and
Rinse Flow Cell Procedure.

8. The Air Pump is changed every _______ cycles and is accessed from the ______ of
the Main Unit.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 31 of 34

XS-1000i with Sampler Competency Assessment

Match the reagent with its function:

_____ EPK 1. Lysing reagent used for differential and optical WBC

_____ FFD 2. Diluent

_____ FFS 3. Cyanide free hemoglobin reagent

_____ SLS 4. Fluorescent dye used in differential reaction

Match the Menu Option with its function:

_____ Sample Explorer 1. Displays scattergrams & histograms with patient results
_____ Controller 2. Displays patient results in line format
_____ Data Browser 3. Contains such options as Maintenance, Reagent Log, Settings
_____ Auto Rinse 4. Cycles all reagents 3-5 times to obtain a background

Using the terms listed, label the X and Y-axis and cell populations
on the Diff scattergram below:

1. Basophils
2. Eosinophils
3. Fluorescence
4. Ghost Cells
5. Lymphocytes
6. Monocytes
7. Neutrophils
8. Side Scatter

Using the terms listed, label the flagging areas on the Diff scattergram below:

1. IG?
2. NRBC?
3. PC?
4. Blast/Atypical Lympho?
5. LS?
6. Abn/Lympho?

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 32 of 34

XS-1000i with Sampler Competency
Assessment Key

Identify the parts selected below:

Ready LED Power
Switch
Sampler Start
Button Mixing
Arm

Manual Start
Button

1. The sample size for aspiration on the XS is 20 µl or 67 µl in capillary mode.

2. Reagents are stable for 60 days after opening.

3. When performing instrument start up, turn on the IPU first and then the Main Unit.

4. Processing samples in the capillary mode requires a 1:7 dilution.

5. You want to retransmit or reprint a result, but under “Report” it does not give you the
option – why?

i. Last 20 is selected

j. The GP printer is out of paper

k. The sample is not validated

l. Both a and c are correct

6. QC material should be brought to room temperature and hand mixed prior to
processing. Vials are good for 14 [(e-CHECK(XS)] or 7 (e-CHECK) days after
opening.

7. CELLCLEAN™ is 5% Sodium Hypochlorite solution and is used for the Monthly
Rinse Procedure and Rinse Flow Cell Procedure.

8. The Air Pump is changed every 30,000 cycles and is accessed from the top of the
Main Unit.

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 33 of 34

XS-1000i with Sampler Competency Assessment Key

Match the reagent with its function:

2 EPK 1. Lysing reagent used for differential and optical WBC

1 FFD 2. Diluent

4 FFS 3. Cyanide free hemoglobin reagent

3 SLS 4. Fluorescent dye used in differential reaction

Match the Menu Option with its function:

2 Sample Explorer 1. Displays scattergrams & histograms with patient results

3 Controller 2. Displays patient results in line format

1 Data Browser 3. Contains such options as Maintenance, Reagent Log, Settings

4 Auto Rinse 4. Cycles all reagents 3-5 times to obtain a background

Using the terms listed, label the X and Y-axis and cell populations
on the Diff scattergram below:

3 1. Basophils
6 2. Eosinophils
3. Fluorescence
5 7 2 4. Ghost Cells
8 5. Lymphocytes
1 6. Monocytes
4 7. Neutrophils
8. Side Scatter

Using the terms listed, label the flagging areas on the Diff scattergram below:

6 1. IG?
2. NRBC?
1 3. PC?
4 4. Blast/Atypical Lympho?
5. LS?
5 6. Abn/Lympho?

23

One Nelson C. White Parkway, Mundelein, IL 60060 Phone 847-996-4500 · 1-800-3SYSMEX (1-800-379-7639) www.sysmex.com

XS-Series Implementation Manual Section 8: Training Tools & Checklist

Document Number: MKT-30-1009 November 2008 Page 34 of 34

XS-1000i Training Checklist

TRAINEE:

DATE TRAINEE TRAINER

1. Instrument Overview
a. Main Unit - Basic parts and function
b. IPU
c. Reagents

2. Start Up Procedure
a. Power On sequence
b. Log on
c. Check Trap Chamber
d. Background Count

3. Quality Control
a. Processing QC
b. QC Files - F5
c. Input - F9
d. QC File Maintenance

4. IPU
a. Explorer - Find; Validate; Modify; Output
b. Browser - Main; Q-flag; Research
c. Controller - Reagent Log, Remaining Reagent
d. GP Customization
e. Auto Rinse

5. Sample Analysis
a. Manual Mode - F2
b. Sampler Mode - F3 (XS-1000i w/sampler only)
c. Capillary Mode - 1:7 dilution

6. Worklist - if applicable
a. IPU and Controller Setting Change
b. Ward Master
c. Doctor Master
d. Patient Master
e. Building Worklist

7. Maintenance >>Explained and shown in OPS Manual Only<<
a. Daily - Shutdown
b. Monthly Rinse - (1200 cycles)
c. Piercer - Air Pump (30,000 cycles)
d. Reagent replacement

8. Troubleshooting
a. Help - F1
b. Clog Removal
c. Rinse Flow Cell
d. Alarm Reset

9. Principles and Technology
a. CBC Parameters
b. Diff Parameters
c. Flagging - Abnormal vs Suspect

November 2008



SYSMEX XS-1000i MAINTENANCE LOG

Hospital Name - City/State
Serial Number #XXXXXX

XS-1000i DAILY MAINTENANCE Month Year

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Perform Shutdown
Verify Background
Perform Quality Control

Initials

Weekly Maintenance date / initials date / initials date / initials date / initials Monthly Maintenance date / initials
date / initials date / initials date / initials date / initials date / initials
Power Down IPU Perform Monthly Rinse (1200 cycles)

As Needed Maintenance Replacements

Replace Air Pump (30000 cycles)
Replace Piercer (30000 cycles)
Replace fuses

Reviewed by Supervsior Name Date

Sysmex America Inc. XS-1000i v. 1.01 November 2008