Functional Catalog Website page

Functional, Chemical & Top-down Proteomics

Functional & Top-down Proteomics NuGel™ NRicher™ Mx

Building sequence/structure/function relationships Low Abundance Proteome Enrichment
 Enriches & normalizes sub-proteomes
The continuum of protein conformations attributable to  Compress proteome concentrations
post-translational modification and non-covalent  Species and tissue agnostic
interactions produces characteristic functions. Thus,  Composite of the NRicher™ 6 mixed mode beads
functional annotation complements sequence  Suitable for small volume mg scale preps
annotation, but relies in part, on the functional or
structural features of intact, non-denatured proteins. Non-reduced SDS-PAGE Mouse Human
BSG has developed a new method for proteome profiles show that major Plasma Serum
separations based on chemically derived weak affinity high abundance bands are
or imperfect fit interactions. Without the use of greatly reduced. The low
antibodies, progressive displacement allows the beads abundance bands are
to bias for or against certain proteins, without enriched relative to their
compromising protein integrity. high abundance bands,
visually estimated to be 5-
The related NRicher™ products (6 & Mx) are 10X for most bands.
adaptable to any measurable protein function from any Because of its
sample type, to aid in the discovery of new biomarkers. heterogeneity, the IgG
They support all functional, activity-probe, chemical variants co-localize to the
and top-down proteomic applications. So functional same region.
protein attributes, as when the same or similar
underlying sequence can have multiple conformations NRicher™ Mx
and functions, or when different sequences cross-over Eluates
in function, are now open to investigation.

NuGel™ NRicher™ 6 Nricher™ 6

Functional proteomics and enrichment kit NRicher™ 6 Beads A, B, C, L, N, R Functional Activity Profile
Normal Serum (upper panel) vs.
 12 differentiated subproteomes, 6 flow-through Colon Cancer Serum (Lower Panel)
fractions, and 6 elution fractions
AB CL N R

 Uncompromised functional and structural
attributes

 Enrich low abundance functional biomarkers for
sequence and structural annotation

- In a rigorous examination of protein

complexes, about twice the number of

observations were made possible through

sub-proteome bias characteristics of

NRicher™ 6 { doi:10.1038/nature14877 } PEP Functional Activity Analysis, courtesy of ArrayBridge (St. Louis, MO), was
- employed for size separation and electro-eluted into the wells. Each well was

 Kit includes 6 mixed mode bead chemistries monitored for Hexokinase activity using beef extract for cascade enzymes;
NADP reduction being the final reporting measurement. The circled regions are
per prep activities up-regulated after subtraction of background. The observed activities

 All Top-down proteomic applications show a different pattern for each of the 6 NRicher™ beads – designated
A,B,C,L,N,R, and generally a feature pattern distinguishing the normal (upper

panel) from the colon cancer (lower panel) sera.

NuGel™

Polymer Coated Silica Affinity Matrices for Ligand Immobilization & Chemical Proteomics

Product Name Ligand Immobilization
NuGELTM Poly-Epoxy Reactive Group Conditions

Amino Direct Coupling

NuGELTM Poly-Amine Carboxylic Acid, Carbodiiamide reaction, or NaIO4 derived Aldehyde
NuGELTM Poly-Aldehyde Carbohydrate Direct Coupling
Amino

NuGELTM Poly-Hydroxy Amino Carbodiimidazole mediated reaction

NuGELTM Poly-Carboxy Amino Carbodiiamide mediated reaction

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